Journal of Veterinary Diagnostic Investigation最新文献_第6页

Enterotoxemia in a 2-day-old lamb produced by a Clostridium perfringens type D lambda toxin-positive strain.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-02-27 DOI: 10.1177/10406387251320943

Kaylin Moctezuma, Hernando D Acevedo, Eileen E Henderson, Javier Asin, John M Adaska, Francisco A Uzal

{"title":"Enterotoxemia in a 2-day-old lamb produced by a Clostridium perfringens type D lambda toxin-positive strain.","authors":"Kaylin Moctezuma, Hernando D Acevedo, Eileen E Henderson, Javier Asin, John M Adaska, Francisco A Uzal","doi":"10.1177/10406387251320943","DOIUrl":"10.1177/10406387251320943","url":null,"abstract":"Clostridium perfringens type D is a gram-positive bacterium that causes enterotoxemia in sheep, goats, and, less frequently, other animals. This microorganism encodes 2 major toxins, alpha (CPA) and epsilon (ETX). Enterotoxemia occurs when epsilon prototoxin (pETX) is produced in the intestine and is activated by one or more proteases before being absorbed into the general circulation. Traditionally, it was believed that neonatal animals were not susceptible to type D enterotoxemia due to the trypsin-inhibitory action of colostrum in the intestinal tract and the lack of protease activation of pETX, although cases of enterotoxemia have been reported in 2 neonatal goat kids. A 2-d-old lamb, with a history of frailty, hunched posture, shallow breathing, and diarrhea followed by death, was submitted for postmortem examination and diagnostic workup. Autopsy revealed hydropericardium, pulmonary edema, and congested intestines. Histologically, there was pulmonary congestion and edema, and intestinal congestion. C. perfringens type D carrying the gene for lambda toxin was isolated from the small intestine and colon. Both CPA and ETX were detected in the small intestine and colon contents. We speculate that lambda toxin (a protease) activated pETX, and that ETX, in turn, produced enterotoxemia in this lamb.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251320943"},"PeriodicalIF":1.2,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11869215/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Mycoplasmopsis (Mycoplasma) bovis in formalin-fixed, paraffin-embedded bovine lung sections by immunohistochemistry and in situ hybridization.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-02-24 DOI: 10.1177/10406387251322463

Matthew Brown, Zackary Laney, Laila Tabatabai, Anna Hassebroek, Kevin Lahmers, Tessa LeCuyer, Francisco A Uzal, Francisco R Carvallo

{"title":"Detection of Mycoplasmopsis (Mycoplasma) bovis in formalin-fixed, paraffin-embedded bovine lung sections by immunohistochemistry and in situ hybridization.","authors":"Matthew Brown, Zackary Laney, Laila Tabatabai, Anna Hassebroek, Kevin Lahmers, Tessa LeCuyer, Francisco A Uzal, Francisco R Carvallo","doi":"10.1177/10406387251322463","DOIUrl":"10.1177/10406387251322463","url":null,"abstract":"The bovine respiratory disease complex (BRDC) is a multifactorial disease of economic importance in cattle involving viral and bacterial agents and several environmental and host-associated predisposing factors. Mycoplasmopsis (Mycoplasma) bovis is frequently detected in BRDC cases, but the role of this bacterium in the pathogenesis of BRDC is not completely understood. We explored the utility of routine histopathology and compared immunohistochemistry (IHC) and in situ hybridization (ISH) in pneumonic bovine lung tissue samples for the detection of M. bovis infection. Samples were analyzed for M. bovis using mycoplasma bacterial culture (screening test), H&E staining, IHC, and ISH. We found that \"compatible histologic lesions\" are not entirely predictive of the presence of M. bovis on culture, IHC, or ISH, and also that there was no statistical difference between IHC and ISH for detecting M. bovis. We conclude that IHC and ISH can be used interchangeably to detect M. bovis infections in pneumonic bovine lung.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251322463"},"PeriodicalIF":1.2,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11851585/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High prevalence and broad geographic distribution of urine shedding of bovine polyomavirus 1 (Epsilonpolyomavirus bovis) by cattle in Uruguay. 乌拉圭牛尿中牛多瘤病毒 1（Epsilonpolyomavirus bovis）的高流行率和广泛地理分布。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-02-20 DOI: 10.1177/10406387251321421

Matías Castells, Leticia Zarantonelli, Caroline da Silva Silveira, Rodney Colina, Federico Giannitti

{"title":"High prevalence and broad geographic distribution of urine shedding of bovine polyomavirus 1 (Epsilonpolyomavirus bovis) by cattle in Uruguay.","authors":"Matías Castells, Leticia Zarantonelli, Caroline da Silva Silveira, Rodney Colina, Federico Giannitti","doi":"10.1177/10406387251321421","DOIUrl":"10.1177/10406387251321421","url":null,"abstract":"In 2021, bovine polyomavirus 1 (BoPyV1; Polyomaviridae, Epsilonpolyomavirus bovis) was associated with nephritis in an aborted bovine fetus in Uruguay, with renal lesions resembling those typical of polyomavirus-associated nephropathy of humans. Given that little is known about the epidemiology of BoPyV1 infection in cattle, we screened for BoPyV1 in urine samples collected in 2015-2017 from beef and dairy herds from 12 of the 19 departments in Uruguay. We tested for BoPyV1 by PCR in 156 urine pools and 249 individual urine samples from 42 herds. We detected BoPyV1 in 33 of 42 (79%) farms across 100% of the departments studied, in similar proportions in beef (22 of 30; 73%) and dairy (11 of 12; 92%) herds. At the animal level, BoPyV1 was detected in 80 of 249 (32%) animals; this frequency was significantly higher in dairy (51 of 119; 43%) than beef (29 of 130; 22%) cattle, and in cows (36 of 81; 44%) than heifers (32 of 121; 26%). BoPyV1 strains circulating in Uruguay have a high degree (98.7-100%) of sequence identity at the major capsid protein VP1, which is slightly lower (96.2-99.7%) than for strains from other countries. We conclude that shedding of BoPyV1 in the urine of dairy and beef cattle is prevalent and geographically widespread in Uruguay.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251321421"},"PeriodicalIF":1.2,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11843559/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ex situ and in situ demonstration of amyloid fibrils for confirmation of amyloidosis using transmission electron microscopy. 利用透射电子显微镜原位和非原位显示淀粉样纤维，以确认淀粉样变性。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-02-20 DOI: 10.1177/10406387251321415

Robert Polon, Christina R Heard, Omar Gonzales-Viera, Melissa Macías-Rioseco, Aslı Mete, Katherine Watson, Leslie W Woods, Aníbal G Armién

{"title":"Ex situ and in situ demonstration of amyloid fibrils for confirmation of amyloidosis using transmission electron microscopy.","authors":"Robert Polon, Christina R Heard, Omar Gonzales-Viera, Melissa Macías-Rioseco, Aslı Mete, Katherine Watson, Leslie W Woods, Aníbal G Armién","doi":"10.1177/10406387251321415","DOIUrl":"10.1177/10406387251321415","url":null,"abstract":"Confirmation of extracellular amyloid deposition across various animal species and tissue types has been a long-standing challenge in veterinary diagnostic pathology. Transmission electron microscopy (TEM) has historically been used to advance the understanding of amyloid fibril morphology and confirm amyloid fibril deposition when histologic methods provide unclear results. We assessed the feasibility of utilizing TEM for routine confirmation of amyloidosis as an addition to histology. We analyzed ex situ amyloid fibrils with direct, negative-contrast TEM and in situ amyloid fibrils with aldehyde-fixed, plastic-embedding TEM to confirm amyloidosis in a variety of cases in which amorphous extracellular amyloid deposits had been identified by H&E and Congo red staining. We compared the 2 TEM methods and documented amyloid fibril morphology and morphometry in 7 species (goat, guinea pig, kudu, fox, sheep, flamingo, and duck). Ex situ fibrils had helical morphology and widths of 15-18 nm across all species. Fibril crossover distances had more interspecies variation of 60-130 nm, and species could be grouped based on pitch (twist size). Twisting patterns of in situ fibrils could not be visualized, but in situ widths of 10 nm were measured across all species. In 4 different chicken cases, fibrils differing morphologically from amyloid were consistently detected via both TEM methods, suggesting the possibility of a non-amyloid deposit that is commonly diagnosed as amyloidosis based on its histologic appearance. When available, we recommend routine confirmation of amyloid fibril deposition by TEM.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251321415"},"PeriodicalIF":1.2,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11843569/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinicopathologic, molecular, and ultrastructural features of Sarcocystis pinnipedi infection in 2 California sea lions with fatal necrotizing hepatitis.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-02-20 DOI: 10.1177/10406387251321832

Elliott S Chiu, Devinn M Sinnott, Martha A Delaney, Michael M Garner, Lance Adams, Bill Van Bonn, Kathleen M Colegrove, Katie Haman, Anibal G Armién, Karen Shapiro

{"title":"Clinicopathologic, molecular, and ultrastructural features of Sarcocystis pinnipedi infection in 2 California sea lions with fatal necrotizing hepatitis.","authors":"Elliott S Chiu, Devinn M Sinnott, Martha A Delaney, Michael M Garner, Lance Adams, Bill Van Bonn, Kathleen M Colegrove, Katie Haman, Anibal G Armién, Karen Shapiro","doi":"10.1177/10406387251321832","DOIUrl":"10.1177/10406387251321832","url":null,"abstract":"Sarcocystis pinnipedi is an apicomplexan protozoal parasite that was first recognized during a mass mortality event in juvenile grey seals (Halichoerus grypus) in the northwest Atlantic Ocean. Since its identification, this parasite has been reported in various pinniped species and has been associated with fatal necrotizing hepatitis. Little is known of the host range of S. pinnipedi. Here we report 2 cases of California sea lions (Zalophus californianus) in managed care that died following an 8-d history of inappetence, vomiting, diarrhea, and progressive lethargy with elevated hepatic enzyme activities. Postmortem examination identified hepatitis and icterus. Sarcocystis schizonts and zoites were identified in regions of necrosis. Molecular and ultrastructural findings demonstrated the close relatedness of this Sarcocystis to S. canis, which produces a similar lesion in bears.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251321832"},"PeriodicalIF":1.2,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11843571/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bovine herpesviral meningoencephalitis: large case study and literature review.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-02-18 DOI: 10.1177/10406387251319804

Bethânia S Santos, Ricardo A A Lemos, Raquel R Rech, Claudio S L Barros, Daniel R Rissi

{"title":"Bovine herpesviral meningoencephalitis: large case study and literature review.","authors":"Bethânia S Santos, Ricardo A A Lemos, Raquel R Rech, Claudio S L Barros, Daniel R Rissi","doi":"10.1177/10406387251319804","DOIUrl":"10.1177/10406387251319804","url":null,"abstract":"Bovine herpesviral meningoencephalitis (BHM) is an infectious disease of cattle caused by bovine alphaherpesvirus 1 (BoAHV1; Orthoherpesviridae, Varicellovirus bovinealpha1) or 5 (BoAHV5; Orthoherpesviridae, Varicellovirus bovinealpha5). Cases are frequent in South America, but the disease is sporadic in other countries. Infection typically leads to fatal necrotizing meningoencephalitis that most often affects calves <1-y-old and yearlings under stressful situations, including weaning, large concentrations of animals, transport, and introduction of cattle from other locations into a herd. Clinical disease lasts from 1-10 d and often leads to typical gross and histologic lesions that are more prominent in the frontal lobes of the brain. This particular neuroanatomic distribution of lesions results from retrograde viral infection from the nasal mucosa along the olfactory nerves and bulbs with subsequent spread to the rostral cerebrum. Because of its particular geographic distribution, many aspects of BHM are still poorly documented in the English language veterinary literature, and thus a comprehensive review of the disease is warranted. Here we review the main aspects of BHM, including its historical timeline, etiology, pathogenesis, epidemiology, clinical and pathologic findings, diagnosis, and control and prophylaxis.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251319804"},"PeriodicalIF":1.2,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11836966/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143449192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Campylobacter jejuni hepatitis in a horse: case report and literature review. 一匹马的空肠弯曲菌肝炎：病例报告和文献综述。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-02-18 DOI: 10.1177/10406387251320604

Melissa Macías-Rioseco, Jennine Ochoa, Eunju April Choi, Patricia Blanchard, Robert B Moeller, Francisco A Uzal

{"title":"Campylobacter jejuni hepatitis in a horse: case report and literature review.","authors":"Melissa Macías-Rioseco, Jennine Ochoa, Eunju April Choi, Patricia Blanchard, Robert B Moeller, Francisco A Uzal","doi":"10.1177/10406387251320604","DOIUrl":"10.1177/10406387251320604","url":null,"abstract":"Campylobacter spp. can cause gastroenteritis, hepatitis, bacteremia, and abortions in domestic animals and humans. Some Campylobacter spp. are zoonotic. To our knowledge, hepatitis caused by Campylobacter jejuni has not been reported in horses. Here we present a case of acute necrosuppurative hepatitis caused by C. jejuni infection in a 3-y-old gelding, and we review the literature on C. jejuni infections in various animal species. The horse had a one-week history of weight loss and weakness before becoming recumbent and dying. Grossly, the liver had rounded edges and was mottled. There were ecchymoses on the gastric serosa, and a large amount of mucoid, pale, green-to-yellow content adhered to the mucosa of the small and large intestines. Microscopically, random areas in the liver were necrotic and infiltrated by large numbers of neutrophils, and fewer lymphocytes and plasma cells. Other changes in the liver included neutrophilic cholangitis with bile duct hyperplasia in portal areas, canalicular cholestasis, and mild portal fibrosis. The lamina propria, and occasionally the submucosa, of the small intestine and colon was infiltrated by large numbers of lymphocytes and plasma cells, and there was multifocal crypt necrosis. C. jejuni was recovered in pure culture from the liver.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251320604"},"PeriodicalIF":1.2,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11836962/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143449260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emergence of hemorrhagic septicemia caused by MLST type ST64 Pasteurella multocida in a European fallow deer population in Hungary.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-02-14 DOI: 10.1177/10406387251320602

Károly Erdélyi, Krisztina Pintér, Boglárka Pollák, Erika Bakcsa, Levente Szeredi, Pál Lehotzky, Tibor Magyar

{"title":"Emergence of hemorrhagic septicemia caused by MLST type ST64 Pasteurella multocida in a European fallow deer population in Hungary.","authors":"Károly Erdélyi, Krisztina Pintér, Boglárka Pollák, Erika Bakcsa, Levente Szeredi, Pál Lehotzky, Tibor Magyar","doi":"10.1177/10406387251320602","DOIUrl":"10.1177/10406387251320602","url":null,"abstract":"A sudden mass mortality involving 44 fallow deer (Dama dama) occurred in September 2020 at a game management area near Budapest, Hungary. The die-off affected both sexes and all age classes equally. On postmortem examination of 2 subadults and 1 fawn, we found average body condition, diffuse subcutaneous edema, swelling of peripheral lymph nodes, marked hemorrhages in lymph nodes along the esophagus and trachea, and left ventricular dilation. We isolated Pasteurella-like colonies from spleen, liver, small intestine, lung, and brain samples of 7 animals, and identified them as P. multocida serotype B:2 by amplifying kmt1, toxA, and hyaC-hyaD genes, capsular typing by PCR, and serotyping by agar gel diffusion precipitation test, which established the diagnosis of hemorrhagic septicemia as the cause of the outbreak. By further analysis, we determined that all of our P. multocida isolates encoded ptfA, fimA, hsf-2, nanH, hgbA, and pfhA, but we did not detect genes encoding toxA, hsf-1, tbpA, or tadD. We identified our strains by RIRDC MLST as sequence type (ST)122, characteristic for strains causing hemorrhagic septicemia, while using multi-host MLST analysis we assigned these strains to ST64 clustering with P. multocida strains from the 2015 hemorrhagic septicemia outbreak of saiga antelopes in Kazakhstan. We concluded that increased population density and aggregation due to limited drinking water availability played a role in initiating the outbreak.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251320602"},"PeriodicalIF":1.2,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830153/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143425533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pyovagina and sepsis due to Streptococcus didelphis in a Virginia opossum.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-02-12 DOI: 10.1177/10406387251319266

Agustín Rebollada-Merino, Taylor C Chan, Cassandra Guarino, Ryan P Taylor, Gavin R Hitchener

{"title":"Pyovagina and sepsis due to Streptococcus didelphis in a Virginia opossum.","authors":"Agustín Rebollada-Merino, Taylor C Chan, Cassandra Guarino, Ryan P Taylor, Gavin R Hitchener","doi":"10.1177/10406387251319266","DOIUrl":"10.1177/10406387251319266","url":null,"abstract":"Virginia opossums (Didelphis virginiana) are the only native marsupials in the United States of America and Canada. Females have a reproductive tract consisting of a complex duplex (bifurcated) reproductive system, with 2 ovaries, 2 uteri and uterine tubes, and a vaginal complex composed of 2 lateral vaginae, vaginal sinuses, and a urogenital sinus. Few conditions of the reproductive tract have been described in these marsupials. A 2-y-old, female Virginia opossum was presented because of hematuria and was found dead shortly after admission. The autopsy revealed left lateral vaginal torsion and pyovagina. Streptococcus didelphis was isolated and identified from the lateral vaginal exudate, blood, and liver using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Histologic examination confirmed suppurative and lymphoplasmacytic vaginitis, endometritis, cystitis, pyelonephritis and interstitial nephritis, portal hepatitis with fibrosis, myocarditis, and adrenalitis with intralesional gram-positive cocci. These results suggest ascending S. didelphis infection of the lateral vagina with subsequent torsion, bacterial translocation, bacteremia, and sepsis as the cause of death.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251319266"},"PeriodicalIF":1.2,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11822774/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143408634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-grade astrocytoma in a sheep: clinical, pathology, and genetic investigations.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-02-12 DOI: 10.1177/10406387251318694

Sebastian A Mignacca, Timm Konold, Elena Gallisai, Caterina Maestrale, Ana Gomez-Vitores, Alejandro Nunez

{"title":"High-grade astrocytoma in a sheep: clinical, pathology, and genetic investigations.","authors":"Sebastian A Mignacca, Timm Konold, Elena Gallisai, Caterina Maestrale, Ana Gomez-Vitores, Alejandro Nunez","doi":"10.1177/10406387251318694","DOIUrl":"10.1177/10406387251318694","url":null,"abstract":"Primary neoplastic diseases of the CNS in livestock are rare compared to companion animals. Here we describe the clinical, pathologic, and genetic findings in an adult sheep with a high-grade cerebral astrocytoma. Clinically, apathy, ataxia, bilateral amaurosis, and horizontal resting nystagmus were observed. The signs were suggestive of a space-occupying lesion affecting the vestibular and ascending reticular activating systems. The CSF had high total protein, hypercellularity, and pleocytosis. Macroscopically, a friable, gray-pink, ovoid mass (6 × 3 × 4 cm) was observed within most of the right parietal, occipital, and temporal cerebral subcortical areas, extended into the thalamus, hippocampus, and the cerebral peduncles, and reached the ipsilateral choroid plexus and the lateral ventricles. Histologically, the well-delimited, unencapsulated lesion consisted of proliferating neoplastic spindloid-to-polygonal cells mainly arranged in streams and bundles supported by minimal fibrous stroma, and random areas of coagulative necrosis and hemorrhages without pseudopalisading in the center of the mass. Immunohistochemistry revealed strong immunolabeling of spindle cells for S100, vimentin, and glial fibrillary acidic protein. Immunolabeling for neuron-specific enolase was restricted to trapped neurons; oligodendrocyte transcription factor 2 (OLIG2), pan-cytokeratins, and SOX10 were immunonegative. TP53 gene sequencing of the neoplasm did not demonstrate any point mutations involving exons 2-11.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251318694"},"PeriodicalIF":1.2,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11822775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143408618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0