Journal of Veterinary Diagnostic Investigation最新文献_第6页

Acute fatal Leptospira kirschneri infection in suckling lambs and calves in Uruguay. 乌拉圭哺乳羔羊和小牛急性致死性克氏钩端螺旋体感染。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-05-12 DOI: 10.1177/10406387251341234

Mizael Machado, Camila Ciuffo, Federico Giannitti, Caroline da Silva Silveira, Yisell Perdomo, Cintia R R Queiroz-Machado, Rafael Carriquiry, Leticia Zarantonelli, Alejo Menchaca

{"title":"Acute fatal Leptospira kirschneri infection in suckling lambs and calves in Uruguay.","authors":"Mizael Machado, Camila Ciuffo, Federico Giannitti, Caroline da Silva Silveira, Yisell Perdomo, Cintia R R Queiroz-Machado, Rafael Carriquiry, Leticia Zarantonelli, Alejo Menchaca","doi":"10.1177/10406387251341234","DOIUrl":"10.1177/10406387251341234","url":null,"abstract":"Leptospira kirschneri is an emerging human and animal pathogen. Here we describe 2 unrelated outbreaks of acute fatal leptospirosis caused by L. kirschneri in suckling lambs and calves diagnosed following a flooding event in the spring of 2023 in northern Uruguay. In outbreak 1, the sheep flock was raised in a low-lying area previously used for rice cultivation; 20 lambs died after developing apathy, jaundice, and hemoglobinuria. In outbreak 2, 2 calves were found dead. At autopsy, 4 lambs and 2 calves had marked jaundice, hemoglobinuria, dark-red kidneys, and hepatomegaly. Microscopically, we found marked hemoglobinuric nephrosis, lymphohistiocytic tubulointerstitial nephritis, hepatocellular dissociation, and random hepatocellular necrosis with periportal lymphohistiocytic hepatitis and canalicular cholestasis. Positive immunostaining for Leptospira sp. was found in the liver (lamb C), both the liver and kidney (calf B), and the liver (calf A). Leptospira kirschneri was PCR-confirmed using kidney and liver samples from the autopsied animals. Acute leptospirosis caused by L. kirschneri has not been reported previously in sheep and cattle, to our knowledge. Our findings highlight that L. kirschneri infection can cause acute leptospirosis in unvaccinated lambs and calves.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"679-683"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075170/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144007532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of fibrinogen Aα-chain as an amyloid precursor protein in a case of canine coronary artery amyloidosis. 纤维蛋白原a α-链在犬冠状动脉淀粉样变性中作为淀粉样前体蛋白的鉴定。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-04-23 DOI: 10.1177/10406387251331933

Susumu Iwaide, Garrett L Wachoski-Dark, Tomoaki Murakami, Niki Sedghi Masoud, Yoshiyuki Itoh, Miki Hisada, Kumiko Kimura, Tomoyuki Shibahara, Makoto Haritani, Jeff L Caswell

{"title":"Identification of fibrinogen Aα-chain as an amyloid precursor protein in a case of canine coronary artery amyloidosis.","authors":"Susumu Iwaide, Garrett L Wachoski-Dark, Tomoaki Murakami, Niki Sedghi Masoud, Yoshiyuki Itoh, Miki Hisada, Kumiko Kimura, Tomoyuki Shibahara, Makoto Haritani, Jeff L Caswell","doi":"10.1177/10406387251331933","DOIUrl":"10.1177/10406387251331933","url":null,"abstract":"Spontaneous amyloid deposition occurs in the coronary arteries in older dogs, but the pathogenesis, including the precursor protein, is not known. We conducted proteome analysis using liquid chromatography-tandem mass spectrometry to identify the amyloid precursor protein in a 13-y-old male mixed-breed dog with amyloid deposition in intramural coronary arteries, and identified fibrinogen Aα-chain. This form of amyloidosis is characterized by the deposition of amyloid derived from fibrinogen Aα-chains, which has been reported previously only in humans and Japanese squirrels. Within amyloid deposits in the intramural coronary arteries of our canine case, the αC connector region was identified in abundance, whereas in humans and Japanese squirrels, the amyloid arises from the αC domain. Thus, even if amyloid is formed by the same protein, the amyloid-forming peptide and the anatomic site of deposition in our case differed from that in humans and Japanese squirrels.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"689-693"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12018354/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144032864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bilateral intraocular choristoma in a 2-day-old foal. 2日龄马驹双侧眼内脉络膜瘤。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-03-11 DOI: 10.1177/10406387251324997

Jasmine Morgan, Gillian Curtis Shaw, Jaime Weisman, Thomas Cecere, Francisco R Carvallo-Chaigneau

引用次数: 0

Canine polyostotic B-cell lymphoma: a case with clinical, immunohistochemical, and flow cytometric characterization, and review of the literature. 犬多骨增生b细胞淋巴瘤：一例临床、免疫组织化学和流式细胞术特征，并复习文献。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-03-21 DOI: 10.1177/10406387251329020

Matthew Kornya, Connor Bryant, Brandon Lillie, Sebastien Sanz, Kristiina Ruotsalo, Dorothee Bienzle

{"title":"Canine polyostotic B-cell lymphoma: a case with clinical, immunohistochemical, and flow cytometric characterization, and review of the literature.","authors":"Matthew Kornya, Connor Bryant, Brandon Lillie, Sebastien Sanz, Kristiina Ruotsalo, Dorothee Bienzle","doi":"10.1177/10406387251329020","DOIUrl":"10.1177/10406387251329020","url":null,"abstract":"An 8-mo-old Mastiff-cross dog with bone pain and lytic-proliferative lesions in the radius, ulna, femur, vertebral spinous processes, and ribs, was diagnosed with lymphoma. The dog also had anemia and thrombocytopenia, and atypical circulating lymphocytes were identified as B cells by flow cytometry. Due to the multicentric, rapidly progressive disease, the dog was euthanized. Postmortem examination confirmed extensive bone replacement by lymphoma, and infiltration of lymph nodes, spleen, and liver. Histomorphology and immunohistochemistry showed a diffuse large B-cell lymphoma that was immunopositive for PAX5 and CD20, and immunonegative for CD3. Lymphoma of bone is rare in dogs and humans, and is most frequently reported in pediatric individuals. Including our case, 7 of 14 reported cases occurred in dogs <2-y-old, and all but 1 had polyostotic disease. Long bones, ribs, and vertebrae were affected most often, and the distal metaphyseal region was targeted in long bones. Visceral and nodal tissue infiltration was common, and all tumors had a diffuse architecture. Most dogs with polyostotic lymphoma were euthanized at the time of diagnosis, and survival was <6 wk in dogs that were treated with chemotherapy or surgery.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"697-704"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11948266/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143676964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiple spinal nerve sheath tumors in a dog resembling schwannomatosis in humans. 狗的多发性脊髓神经鞘肿瘤类似于人类的神经鞘瘤病。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-05-07 DOI: 10.1177/10406387251329049

Jun Lai Tse, Agustín Rebollada-Merino, Martí Pumarola, Ana Liza Ortiz, Cristina Toni

引用次数: 0

Concurrent pancreatic exocrine hyperplasia and adenomas in a Eurasian otter with pleuropneumonia and urolithiasis. 并发胰腺外分泌增生和腺瘤的欧亚水獭胸膜肺炎和尿石症。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-05-13 DOI: 10.1177/10406387251338327

Jeong-Eun Yeom, Min-Gyeong Seo, Kook-Young Han, Sang-Hyun Kim, Eun-Joo Lee, Kyu-Shik Jeong, Il-Hwa Hong

引用次数: 0

Characterization of amyloid deposits in canine mammary neoplasms. 犬乳腺肿瘤中淀粉样蛋白沉积的特征。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-04-01 DOI: 10.1177/10406387251328323

Yuka Kato, Natsumi Kobayashi, Susumu Iwaide, Shumpei Hisamoto, Satoshi Koyama, Yoshiyuki Itoh, Miki Hisada, Noboru Machida, Kohji Nomura, Tomoaki Murakami

{"title":"Characterization of amyloid deposits in canine mammary neoplasms.","authors":"Yuka Kato, Natsumi Kobayashi, Susumu Iwaide, Shumpei Hisamoto, Satoshi Koyama, Yoshiyuki Itoh, Miki Hisada, Noboru Machida, Kohji Nomura, Tomoaki Murakami","doi":"10.1177/10406387251328323","DOIUrl":"10.1177/10406387251328323","url":null,"abstract":"Mammary tumor-associated amyloidosis in dogs has been reported in several studies, but its incidence and predisposing conditions have not been investigated. We examined 252 mammary masses from 183 dogs to determine the prevalence of amyloid deposition and elucidate etiologic factors. Histologically, amyloid deposition was observed in 88 of 252 (35%) mammary masses. Amyloid deposition patterns were categorized into 3 types: stromal deposition (ST), deposition in corpora amylacea (CA), and precipitation within luminal secretions (PR). Of the 88 amyloid deposition cases, 49 were intra-tumoral, especially for ST-type amyloid deposits, which were all intra-tumoral. Immunohistochemistry and mass spectrometry revealed that ST-type amyloid deposits were positive for either serum amyloid A or α-S1-casein, depending on the sample; all CA- and PR-type amyloid deposits were positive for α-S1-casein. Statistically, intra-tumoral ST-type amyloid deposition was significantly more frequent in histologic grade II (p = 0.009) or III (p < 0.001) mammary tumors. In contrast, amyloid deposition in extra-tumoral tissues is significantly associated with aging (p = 0.002). Our findings suggest that the disruption of proteostasis in the canine mammary gland associated with tumorigenesis and aging can readily induce amyloid formation.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"574-583"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11966624/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143763646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytology and histology of a high-grade oligodendroglioma with embryonal features in a dog. 犬胚胎特征的高级别少突胶质细胞瘤的细胞学和组织学研究。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-04-16 DOI: 10.1177/10406387251331553

Flavio H Alonso, Aria Phan, Kelly O'Connell, Demitria M Vasilatis

引用次数: 0

Comparison of the determination of cortisol in canine saliva by chemiluminescence immunoassay and liquid chromatography-tandem mass spectrometry. 化学发光免疫分析法与液相色谱-串联质谱法测定犬唾液中皮质醇的比较。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-05-14 DOI: 10.1177/10406387251327413

Maike Schroers, Yuri Zablotski, Andrea Meyer-Lindenberg

{"title":"Comparison of the determination of cortisol in canine saliva by chemiluminescence immunoassay and liquid chromatography-tandem mass spectrometry.","authors":"Maike Schroers, Yuri Zablotski, Andrea Meyer-Lindenberg","doi":"10.1177/10406387251327413","DOIUrl":"10.1177/10406387251327413","url":null,"abstract":"We determined saliva cortisol concentrations (SCCs) in samples from 36 dogs using a chemiluminescence immunoassay (CLIA) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) before and after a 10-min veterinary visit. The median cortisol concentration of the 77 samples analyzed with CLIA was 1.34 nmol/L, and the concentration of samples analyzed with LC-MS/MS was 1.30 nmol/L. With an intercept (a) of -0.06 (95% CI: -0.16 to 0.02) and a slope (b) of 0.97 (95% CI: 0.90-0.97) the Passing-Bablok regression revealed that there were no significant systematic and proportional differences between the 2 methods. In the Bland-Altman analysis, the bias was 0.08 nmol/L (95% CI: -0.38 to 0.54 nmol/L) between the 2 methods. Comparing the hormone levels in the saliva of 36 dogs before and after a veterinary visit, the CLIA method had a mean difference of 0.03 nmol/L (95% CI: -1.72 to 4.75 nmol/L; p = 0.35) and the LC-MS/MS method a mean difference of 0.12 nmol/L (95% CI 95%: -2.43 to 4.40 nmol/L; p = 0.62). Comparison of cortisol changes before (p = 0.86) and after (p = 0.79) the veterinary visit revealed no differences in using either the CLIA or LC-MS/MS methods. There was also no difference in the assessment of hormone increase or decrease between CLIA (p = 0.72) and LC-MS/MS (p = 0.17). Overall, the measurement of SCC in dogs by either CLIA or LC-MS/MS provided comparable results.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"539-544"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12081392/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laboratory assessment of recovery of porcine circovirus 2 and porcine reproductive and respiratory syndrome virus using 2 commercial hollow-fiber ultrafilters. 使用两种商用中空纤维超滤器回收猪圆环病毒 2 和猪繁殖与呼吸综合征病毒的实验室评估。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-05-01 Epub Date: 2025-02-28 DOI: 10.1177/10406387251322506

Aaron D Firnstahl, Gabrielle E Doughan, Sarah A Opelt, Rachel M Cook, Joseph A Heffron, Karen M Krueger, Mark A Borchardt, Locke A Karriker, Joel P Stokdyk, Tucker R Burch

{"title":"Laboratory assessment of recovery of porcine circovirus 2 and porcine reproductive and respiratory syndrome virus using 2 commercial hollow-fiber ultrafilters.","authors":"Aaron D Firnstahl, Gabrielle E Doughan, Sarah A Opelt, Rachel M Cook, Joseph A Heffron, Karen M Krueger, Mark A Borchardt, Locke A Karriker, Joel P Stokdyk, Tucker R Burch","doi":"10.1177/10406387251322506","DOIUrl":"10.1177/10406387251322506","url":null,"abstract":"Groundwater near swine farms is an uninvestigated reservoir for porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circoviruses (PCVs). Enteric microorganisms are often collected from groundwater via dead-end ultrafiltration, but recovery of PRRSV and PCV with this method has not been assessed. We recovered PRRSV2 and PCV2 by dead-end ultrafiltration followed by polyethylene glycol (PEG) precipitation, nucleic acid extraction, and reverse-transcription quantitative real-time PCR. We also compared 2 commercial hemodialysis ultrafilters (Asahi Kasei Rexeed-25A, Nipro Elisio-25H) and compared PRRSV2 recovery in these filters to other waterborne microorganisms. On average, 8 ± 1% of PRRSV2 was recovered by dead-end ultrafiltration and PEG precipitation, compared to 25 ± 6% for adenovirus 41. Full-process recovery of bacteria in the same filters was 5-15%; Cryptosporidium parvum recovery was 42 ± 12%. PCV2 was detected in 4 of 12 replicate filters, but low stock concentrations precluded quantitative recovery estimates. Elisio-25H ultrafilters performed similarly to Rexeed-25A filters for all organisms tested and is an effective replacement for the Rexeed-25A, which is no longer available in the United States. Our recovery of PRRSV2 and PCV2 by dead-end ultrafiltration in the laboratory suggests that PRRSV2 detection limits are as low as 3-50 genomic copies/L in sample volumes of 100-1,500 L. Based on quantitative microbial risk assessment, these concentrations are relevant to PRRSV2 infection rates in the U.S. swine herd.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"439-447"},"PeriodicalIF":1.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11871577/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0