Journal of Veterinary Diagnostic Investigation最新文献_第6页

Pancreatitis, panniculitis, and polyarthritis syndrome in a dog with hyalinizing pancreatic adenocarcinoma. 一只患有透明化胰腺腺癌的狗出现了胰腺炎、泛发性胰腺炎和多关节炎综合征。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2024-11-01 Epub Date: 2024-09-04 DOI: 10.1177/10406387241267870

Abby Hagelskamp, Amelia G White, Jeremy Gallman, Cierra Starbird, Rachel L A L T Neto

{"title":"Pancreatitis, panniculitis, and polyarthritis syndrome in a dog with hyalinizing pancreatic adenocarcinoma.","authors":"Abby Hagelskamp, Amelia G White, Jeremy Gallman, Cierra Starbird, Rachel L A L T Neto","doi":"10.1177/10406387241267870","DOIUrl":"10.1177/10406387241267870","url":null,"abstract":"A 10-y-old spayed female Cavalier King Charles Spaniel dog was presented to the Veterinary Teaching Hospital because of recurrent chronic abscesses on the distal pelvic limbs, fever, lethargy, lameness of unknown etiology, and chronic pancreatitis. Sterile nodular panniculitis was diagnosed after an extensive workup, and the dog initially responded to immunosuppressive therapy, but relapse and spread of cutaneous lesions and acute lameness occurred after 11 mo, and euthanasia was elected. Postmortem examination confirmed hyalinizing pancreatic adenocarcinoma with pancreatitis, panniculitis, polyarthritis (PPP), and osteomyelitis. Histopathology and bacterial and fungal cultures were supportive of a sterile process, specifically the PPP syndrome, which is a rare, potentially life-threatening, systemic manifestation of pancreatic disease in both people and animals. To our knowledge, a clinicopathologic description of a hyalinizing pancreatic adenocarcinoma associated with this rare syndrome has not been reported previously in a dog.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"886-890"},"PeriodicalIF":1.2,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11529078/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel genotyping assay for a 212-kb deletion from the BBS9 gene, and frequency of the allele in pig populations in Vietnam. 针对 BBS9 基因 212-kb 缺失的新型基因分型测定法，以及该等位基因在越南猪群中的频率。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2024-11-01 Epub Date: 2024-09-26 DOI: 10.1177/10406387241282082

Nguyen H Tinh, Nguyen V Hop, Pham T Phuong, Trinh L H Tam, Nguyen B Quoc, Trinh H Son, Anh P N Bui

{"title":"Novel genotyping assay for a 212-kb deletion from the BBS9 gene, and frequency of the allele in pig populations in Vietnam.","authors":"Nguyen H Tinh, Nguyen V Hop, Pham T Phuong, Trinh L H Tam, Nguyen B Quoc, Trinh H Son, Anh P N Bui","doi":"10.1177/10406387241282082","DOIUrl":"10.1177/10406387241282082","url":null,"abstract":"Piglet lethality is one of the major concerns in pig breeding programs. Deletion of a 212-kb region within the Bardet-Biedl syndrome 9 (BBS9) gene has been linked to a reduction in the number of piglets born alive per litter. The BBS9 mutant gene carrier-by-carrier mating scheme could result in mummification of piglets carrying 2 copies of the BBS9 mutant allele, which ultimately affects the reproductive performance of the sow. Our aim was to develop a simple, rapid, and cost-efficient method that could be applied in a BBS9 mutant gene carrier screening program in low- and middle-income countries within basic laboratory settings. Here, we report an optimized multiplex PCR assay that we have established successfully for detection of a 212-kb deletion within the BBS9 genomic sequence. We genotyped 420 animals from Yorkshire, Duroc, and Landrace purebred populations in Vietnam. We found that while the BBS9 mutant allele was not identified in Duroc pigs, the frequency of BBS9 carriers was 10% in both Yorkshire and Landrace populations. We subsequently validated our results using Sanger sequencing. Our multiplex PCR method could be utilized as a BBS9 screening test in pig breeding programs.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"847-851"},"PeriodicalIF":1.2,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11512462/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mass spectrometry in animal health laboratories: recent history, current applications, and future directions. 动物保健实验室中的质谱分析：近代历史、当前应用和未来方向。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2024-11-01 Epub Date: 2024-08-22 DOI: 10.1177/10406387241270071

Michael S Filigenzi

引用次数: 0

Evaluation of an in-house indirect immunoperoxidase test for detection of antibodies against African swine fever virus. 评估用于检测非洲猪瘟病毒抗体的内部间接免疫过氧化物酶试验。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2024-11-01 Epub Date: 2024-09-06 DOI: 10.1177/10406387241267883

Ping Wu, Aric J McDaniel, Yelitza Y Rodríguez, Leslie Blakemore, Kate R Schumann, Chungwon J Chung, Wei Jia

{"title":"Evaluation of an in-house indirect immunoperoxidase test for detection of antibodies against African swine fever virus.","authors":"Ping Wu, Aric J McDaniel, Yelitza Y Rodríguez, Leslie Blakemore, Kate R Schumann, Chungwon J Chung, Wei Jia","doi":"10.1177/10406387241267883","DOIUrl":"10.1177/10406387241267883","url":null,"abstract":"African swine fever (ASF) is a high-consequence transboundary animal disease caused by African swine fever virus (ASFV). Given that vaccines are not widely available, ASFV detection, including by molecular and serologic assays, is paramount to efficacious control and mitigation of ASF. ASFV-specific antibodies can be detected as early as 7-10 d postinfection in infected animals and may persist for several months or longer. Accurate detection of ASFV-specific antibody is critical for the identification of chronically infected, subclinically infected, or recovered animals. ELISAs are commonly used for the rapid screening of large numbers of animals for ASFV antibodies. The World Organisation for Animal Health recommends that ELISA-positive results should be confirmed with a second serologic method, such as an indirect immunofluorescent assay, indirect immunoperoxidase test (IPT), or immunoblot test. Commercial kits are not available for those tests. We developed and validated an in-house IPT by using a currently circulating genotype II ASFV strain as antigen. The sensitivity and specificity of the in-house IPT are comparable to the reference IPT developed by an international ASFV reference laboratory and superior to a commercial blocking ELISA.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"870-873"},"PeriodicalIF":1.2,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11529064/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142145909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Congenital pulmonary airway malformation in a cat. 一只猫的先天性肺气道畸形。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2024-10-29 DOI: 10.1177/10406387241293134

Deborah L A Chong, Christiane V Löhr, Pun Sriboonyapirat, Kurt J Williams

引用次数: 0

Systemic Acanthamoeba T17 infection in a free-ranging two-toed sloth: case report and literature review of infections by free-living amebas in mammals. 自由活动的双趾树懒全身感染阿卡阿米巴 T17：病例报告和哺乳动物自由生活阿米巴感染的文献综述。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2024-10-29 DOI: 10.1177/10406387241292346

Daniel Felipe Barrantes Murillo, Alexis Berrocal, Ibne Karim M Ali, Francisco A Uzal

{"title":"Systemic Acanthamoeba T17 infection in a free-ranging two-toed sloth: case report and literature review of infections by free-living amebas in mammals.","authors":"Daniel Felipe Barrantes Murillo, Alexis Berrocal, Ibne Karim M Ali, Francisco A Uzal","doi":"10.1177/10406387241292346","DOIUrl":"10.1177/10406387241292346","url":null,"abstract":"A free-ranging, adult female two-toed sloth (Choloepus hoffmanni) was brought to a wildlife rescue center in Costa Rica with ocular and auricular myiasis and numerous skin lesions. After one month of unsuccessful systemic and topical antimicrobial treatment, the patient died. A postmortem examination was performed, and tissues were examined histologically, confirming disseminated amebic infection with intralesional trophozoites and cysts in the lungs, liver, eye, heart, spleen, and stomach. Immunohistochemistry identified the ameba as Acanthamoeba sp. A multiplex real-time PCR assay, 18S ribosomal DNA PCR, and sequencing performed on formalin-fixed, paraffin-embedded lung tissue confirmed the Acanthamoeba T17 genotype. The Acanthamoeba genus is in the group of free-living amebas that cause infection in humans and animals, and it is ubiquitous in the environment. Acanthamoeba T17 has been isolated from water and soil, but to our knowledge, this genotype has not been implicated in infections of animals previously and has not been reported from Costa Rica. Systemic Acanthamoeba infection has not been described in sloths previously. We provide a comprehensive literature review describing infections by free-living amebas of the genus Acanthamoeba spp., Balamuthia spp., and Naegleria spp. in domestic, zoo, and wild mammals.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387241292346"},"PeriodicalIF":1.2,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11559879/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of blood meals from stable flies to evaluate the bovine leukemia virus infection status in cattle herds: a pilot study. 利用厩蝇血餐评估牛群的牛白血病病毒感染状况：一项试点研究。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2024-10-27 DOI: 10.1177/10406387241293081

Kaori Shimizu, Chikahiro Mori, Ayaka Okada, Yasuo Inoshima

{"title":"Use of blood meals from stable flies to evaluate the bovine leukemia virus infection status in cattle herds: a pilot study.","authors":"Kaori Shimizu, Chikahiro Mori, Ayaka Okada, Yasuo Inoshima","doi":"10.1177/10406387241293081","DOIUrl":"10.1177/10406387241293081","url":null,"abstract":"The incidence of enzootic bovine leukosis (EBL), a type of B-cell lymphoma, is increasing in Japan. EBL is caused by bovine leukemia virus (BLV; Retroviridae, Deltaretrovirus bovleu) infection and is diagnosed by detecting antibodies against BLV in milk and blood or BLV DNA in blood. We assessed the feasibility of using stable flies (Stomoxys calcitrans) as a sampling tool to assess BLV infection status in cattle herds. First, we collected blood from 3 cattle herds and, based on the measurement of BLV-proviral load (PVL) by quantitative real-time PCR (qPCR), identified 1) a BLV-free herd, 2) a herd with a low prevalence of BLV-infected cattle and low PVL, and 3) a herd wherein half of the cattle were BLV-infected with low-to-high PVLs. Next, we collected stable flies from the 3 herds, extracted DNA from their blood meals, analyzed it for BLV DNA, and measured the BLV PVL. Cattle DNA and BLV DNA, but not other mammalian DNA, were successfully detected by digestion of the flies. Based on fly blood meal qPCR, we identified one herd as BLV-free and the other 2 herds as having <50% prevalence of BLV-infected cattle with low PVLs. Our fly results were not consistent with preliminary BLV-PVL measurements on cattle blood. Our pilot study indicated that, to assess the feasibility of a stable fly blood meal test as an alternative technique for evaluating BLV infection status in dairy and beef cattle, additional investigations involving more cattle herds and stable flies are needed.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387241293081"},"PeriodicalIF":1.2,"publicationDate":"2024-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11559776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determining diagnostic sensitivity loss limits for sample pooling in duplex rtPCR surveillance testing: Theileria orientalis and Anaplasma marginale. 确定双重 rtPCR 监测测试中样本池的诊断灵敏度损失限度：东方壁虱和边缘疟原虫。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2024-10-26 DOI: 10.1177/10406387241287516

Catharine Burgess, S Michelle Todd, Laura Hungerford, Kevin Lahmers

{"title":"Determining diagnostic sensitivity loss limits for sample pooling in duplex rtPCR surveillance testing: Theileria orientalis and Anaplasma marginale.","authors":"Catharine Burgess, S Michelle Todd, Laura Hungerford, Kevin Lahmers","doi":"10.1177/10406387241287516","DOIUrl":"10.1177/10406387241287516","url":null,"abstract":"To expand surveillance testing capacity through sample pooling, a thorough understanding is needed of how sample dilution through pooling affects the sensitivity of candidate assays. We validated a robust and representative framework for assessing the dilution effect of sample pooling using duplex rtPCR surveillance of Theileria orientalis and Anaplasma marginale, both of which are causative agents of severe anemia in cattle and a serious threat to the cattle industry in Virginia and many other states. We used 200 known-positive samples with Ct values representative of typical surveillance results in a series of pools in which we re-tested each sample individually, followed by each sample diluted in equal volumes with negative samples to make pools of 2, 4, 6, 8, and 10 total samples. We compared the Ct values of the individual positives with the Ct values of each pool size to determine if Ct values increase past the limit of detection in the 45-cycle assay. We observed a maximum of 2% sensitivity loss (no more than 2 of 100 samples returned a false-negative result) for both T. orientalis and A. marginale during the pooling series, with lower-than-expected average Ct increase and sensitivity loss. We conclude that pooling up to 10 samples would be acceptable for regional surveillance of T. orientalis and A. marginale using our rtPCR assay. The described strategy is applicable to validate pooling for a wide range of single and duplex rtPCR assays, which could expand efficient disease surveillance.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387241287516"},"PeriodicalIF":1.2,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11559834/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histopathologic and ultrastructural findings in oak (Quercus spp.) toxicity on 2 beef cattle farms in Colorado. 科罗拉多州两个肉牛养殖场橡树（栎属）中毒的组织病理学和超微结构研究结果。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2024-10-15 DOI: 10.1177/10406387241288224

Jorge Mendieta-Calle, Kelly L Hughes, Elizabeth W Howerth, Paula A Schaffer

{"title":"Histopathologic and ultrastructural findings in oak (Quercus spp.) toxicity on 2 beef cattle farms in Colorado.","authors":"Jorge Mendieta-Calle, Kelly L Hughes, Elizabeth W Howerth, Paula A Schaffer","doi":"10.1177/10406387241288224","DOIUrl":"10.1177/10406387241288224","url":null,"abstract":"Natural oak toxicity, a phenomenon sporadically reported in the United States, is due to consumption of any part of most oak trees (Quercus spp.). Ruminants, mainly cattle, are disproportionately susceptible to oak toxicity. Toxicity is attributed to degradation of the oak plant hydrolysable tannins by rumen microbes and enzymes into absorbable low-molecular-weight metabolites, which are postulated to bind and damage endothelial cells by unknown mechanisms. The clinical manifestations of acute toxicosis are nonspecific or broadly suggestive of renal disease due to acute tubular injury. Here we document the clinical, gross, histopathologic, and novel ultrastructural features of natural acute oak nephrotoxicity in 3 beef calves on 2 farms in Colorado, USA. Gross postmortem findings included perirenal edema with renomegaly and hemorrhagic gastroenteritis. Histologically, renal tubular epithelial necrosis was severe, with hemorrhage and intratubular hyaline casts. Transmission electron microscopy revealed extensive involvement of proximal and distal convoluted tubules, with predominantly intact basement membranes, and glomerular and interstitial endothelial injury and necrosis. The ultrastructural details of toxic nephropathy and vasculopathy induced by oak metabolites in natural cases of bovine oak toxicosis have not been described previously, to our knowledge.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387241288224"},"PeriodicalIF":1.2,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11559815/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142468870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intra-abdominal nocardiosis and scedosporiosis in a dog: case report and literature review. 一只狗的腹腔线虫病和头孢子虫病：病例报告和文献综述。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2024-10-14 DOI: 10.1177/10406387241287799

Jessica Rose Lambert, Arthur Colombari Cheng, Laura M Lee, Donna Raiford, Emily Zuber, Erin Kilbane, Eric J Fish, Ewa Królak, Katelyn C Hlusko, Maureen McMichael, Rebecca P Wilkes, Nathan P Wiederhold, Connie F Cañete-Gibas, Daniel Felipe Barrantes Murillo

{"title":"Intra-abdominal nocardiosis and scedosporiosis in a dog: case report and literature review.","authors":"Jessica Rose Lambert, Arthur Colombari Cheng, Laura M Lee, Donna Raiford, Emily Zuber, Erin Kilbane, Eric J Fish, Ewa Królak, Katelyn C Hlusko, Maureen McMichael, Rebecca P Wilkes, Nathan P Wiederhold, Connie F Cañete-Gibas, Daniel Felipe Barrantes Murillo","doi":"10.1177/10406387241287799","DOIUrl":"10.1177/10406387241287799","url":null,"abstract":"A 2-y-old, intact female, mixed-breed dog was presented to the veterinary hospital with abdominal distension, anemia, and lethargy following a chronic history of nonspecific gastrointestinal signs. CBC and serum biochemistry revealed moderate nonregenerative anemia with neutrophilia, hypoalbuminemia, hyperglobulinemia, hypoglycemia, decreased urea and creatinine, and hypercholesterolemia. Abdominal radiographs and ultrasound revealed a large heterogeneous mesenteric mass and ascites. Abdominocentesis confirmed septic peritonitis with filamentous bacteria. Fine-needle aspiration of the mass yielded pyogranulomatous inflammation and hyphae. An exploratory laparotomy revealed a large cranial abdominal mass with granulomas present throughout the abdominal cavity. Due to the poor prognosis and disseminated disease, the owner elected euthanasia. Postmortem and histologic examinations detected intralesional mycetomas and bacterial colonies within the mesenteric masses. 16S ribosomal RNA gene PCR and sequencing using formalin-fixed, paraffin-embedded sections identified Nocardia yamanashiensis, Nocardioides cavernae, and Nocardioides zeicaulis. Fungal culture, PCR, and sequencing confirmed Scedosporium apiospermum. Our report highlights the importance of molecular methods in conjunction with culture and histologic findings for diagnosing coinfections caused by infrequent etiologic agents. Additionally, we provide a comprehensive literature review of Scedosporium apiospermum infections in dogs.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387241287799"},"PeriodicalIF":1.2,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11559917/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142468872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0