Journal of Veterinary Diagnostic Investigation最新文献_第6页

Campylobacter jejuni hepatitis in a horse: case report and literature review. 一匹马的空肠弯曲菌肝炎：病例报告和文献综述。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-05-01 Epub Date: 2025-02-18 DOI: 10.1177/10406387251320604

Melissa Macías-Rioseco, Jennine Ochoa, Eunju April Choi, Patricia Blanchard, Robert B Moeller, Francisco A Uzal

{"title":"Campylobacter jejuni hepatitis in a horse: case report and literature review.","authors":"Melissa Macías-Rioseco, Jennine Ochoa, Eunju April Choi, Patricia Blanchard, Robert B Moeller, Francisco A Uzal","doi":"10.1177/10406387251320604","DOIUrl":"10.1177/10406387251320604","url":null,"abstract":"Campylobacter spp. can cause gastroenteritis, hepatitis, bacteremia, and abortions in domestic animals and humans. Some Campylobacter spp. are zoonotic. To our knowledge, hepatitis caused by Campylobacter jejuni has not been reported in horses. Here we present a case of acute necrosuppurative hepatitis caused by C. jejuni infection in a 3-y-old gelding, and we review the literature on C. jejuni infections in various animal species. The horse had a one-week history of weight loss and weakness before becoming recumbent and dying. Grossly, the liver had rounded edges and was mottled. There were ecchymoses on the gastric serosa, and a large amount of mucoid, pale, green-to-yellow content adhered to the mucosa of the small and large intestines. Microscopically, random areas in the liver were necrotic and infiltrated by large numbers of neutrophils, and fewer lymphocytes and plasma cells. Other changes in the liver included neutrophilic cholangitis with bile duct hyperplasia in portal areas, canalicular cholestasis, and mild portal fibrosis. The lamina propria, and occasionally the submucosa, of the small intestine and colon was infiltrated by large numbers of lymphocytes and plasma cells, and there was multifocal crypt necrosis. C. jejuni was recovered in pure culture from the liver.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"463-466"},"PeriodicalIF":1.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11836962/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143449260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reproductive loss attributed to Lactococcus petauri infection in a black-and-white ruffed lemur. 黑白狐猴因感染 Petauri 乳球菌而丧失生殖能力。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-05-01 Epub Date: 2025-02-28 DOI: 10.1177/10406387251323565

Agustín Rebollada-Merino, Ana Isabel Vela, Rocío Canales, Umberto Romani-Cremaschi, María Ugarte-Ruiz, Arantxa Buendía, Marta Pérez-Sancho, Lucas Domínguez, José Francisco Fernández-Garayzabal, Antonio Rodríguez-Bertos

{"title":"Reproductive loss attributed to Lactococcus petauri infection in a black-and-white ruffed lemur.","authors":"Agustín Rebollada-Merino, Ana Isabel Vela, Rocío Canales, Umberto Romani-Cremaschi, María Ugarte-Ruiz, Arantxa Buendía, Marta Pérez-Sancho, Lucas Domínguez, José Francisco Fernández-Garayzabal, Antonio Rodríguez-Bertos","doi":"10.1177/10406387251323565","DOIUrl":"10.1177/10406387251323565","url":null,"abstract":"Lactococci have been associated with fetal and neonatal infections in humans and cattle. Here we describe a case of reproductive loss attributed to Lactococcus petauri in a lemur. A full-term black-and-white ruffed lemur (Varecia variegata) was found dead in the indoor area of a zoologic exhibit. Classification as a late-term abortion or stillbirth was unclear as the precise gestational time was unknown. A medical checkup of the dam revealed fever and neutrophilic leukocytosis; recovery followed treatment with enrofloxacin. The main histologic findings were placental edema and hemorrhage, hepatic necrosis, desquamated amniotic epithelial cells in alveoli, and subendocardial and myocardial hemorrhages. Tissue Gram stain revealed abundant gram-positive cocci arranged in short chains in the placenta and liver. Toxoplasma gondii was not detected by immunohistochemistry. Bacterial isolates from the placenta and fetal liver were identified as Lactococcus garvieae by MALDI-TOF MS. However, the isolates were found to be L. petauri by determining their in-silico DNA-DNA hybridization and average nucleotide identity values using pairwise comparisons of their whole-genome sequences and the genomes of the type strains. The antimicrobial susceptibility of isolates by the disk diffusion method revealed resistance to tylosin, gentamicin, apramycin, neomycin, amikacin, ampicillin, and florfenicol. We attributed the reproductive loss in this lemur to placental and fetal infection by L. petauri.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"471-474"},"PeriodicalIF":1.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11871578/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Mycoplasmopsis (Mycoplasma) bovis in formalin-fixed, paraffin-embedded bovine lung sections by immunohistochemistry and in situ hybridization. 免疫组织化学和原位杂交技术检测福尔马林固定、石蜡包埋牛肺切片中的牛支原体。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-05-01 Epub Date: 2025-02-24 DOI: 10.1177/10406387251322463

Matthew Brown, Zackary Laney, Laila Tabatabai, Anna Hassebroek, Kevin Lahmers, Tessa LeCuyer, Francisco A Uzal, Francisco R Carvallo

{"title":"Detection of Mycoplasmopsis (Mycoplasma) bovis in formalin-fixed, paraffin-embedded bovine lung sections by immunohistochemistry and in situ hybridization.","authors":"Matthew Brown, Zackary Laney, Laila Tabatabai, Anna Hassebroek, Kevin Lahmers, Tessa LeCuyer, Francisco A Uzal, Francisco R Carvallo","doi":"10.1177/10406387251322463","DOIUrl":"10.1177/10406387251322463","url":null,"abstract":"The bovine respiratory disease complex (BRDC) is a multifactorial disease of economic importance in cattle involving viral and bacterial agents and several environmental and host-associated predisposing factors. Mycoplasmopsis (Mycoplasma) bovis is frequently detected in BRDC cases, but the role of this bacterium in the pathogenesis of BRDC is not completely understood. We explored the utility of routine histopathology and compared immunohistochemistry (IHC) and in situ hybridization (ISH) in pneumonic bovine lung tissue samples for the detection of M. bovis infection. Samples were analyzed for M. bovis using mycoplasma bacterial culture (screening test), H&E staining, IHC, and ISH. We found that \"compatible histologic lesions\" are not entirely predictive of the presence of M. bovis on culture, IHC, or ISH, and also that there was no statistical difference between IHC and ISH for detecting M. bovis. We conclude that IHC and ISH can be used interchangeably to detect M. bovis infections in pneumonic bovine lung.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"448-454"},"PeriodicalIF":1.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11851585/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fatal tracheal collapse and ossification in an osteopenic captive Indian blue peafowl in California. 加利福尼亚一只骨质减少的圈养印第安蓝孔雀的致命气管塌陷和骨化。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-05-01 Epub Date: 2025-03-02 DOI: 10.1177/10406387251323870

Carlos Daniel Gornatti-Churria, Javier Asin, Aníbal G Armién, Veronica Nguyen, Simone T Stoute

引用次数: 0

Ex situ and in situ demonstration of amyloid fibrils for confirmation of amyloidosis using transmission electron microscopy. 利用透射电子显微镜原位和非原位显示淀粉样纤维，以确认淀粉样变性。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-05-01 Epub Date: 2025-02-20 DOI: 10.1177/10406387251321415

Robert Polon, Christina R Heard, Omar Gonzales-Viera, Melissa Macías-Rioseco, Aslı Mete, Katherine Watson, Leslie W Woods, Aníbal G Armién

{"title":"Ex situ and in situ demonstration of amyloid fibrils for confirmation of amyloidosis using transmission electron microscopy.","authors":"Robert Polon, Christina R Heard, Omar Gonzales-Viera, Melissa Macías-Rioseco, Aslı Mete, Katherine Watson, Leslie W Woods, Aníbal G Armién","doi":"10.1177/10406387251321415","DOIUrl":"10.1177/10406387251321415","url":null,"abstract":"Confirmation of extracellular amyloid deposition across various animal species and tissue types has been a long-standing challenge in veterinary diagnostic pathology. Transmission electron microscopy (TEM) has historically been used to advance the understanding of amyloid fibril morphology and confirm amyloid fibril deposition when histologic methods provide unclear results. We assessed the feasibility of utilizing TEM for routine confirmation of amyloidosis as an addition to histology. We analyzed ex situ amyloid fibrils with direct, negative-contrast TEM and in situ amyloid fibrils with aldehyde-fixed, plastic-embedding TEM to confirm amyloidosis in a variety of cases in which amorphous extracellular amyloid deposits had been identified by H&E and Congo red staining. We compared the 2 TEM methods and documented amyloid fibril morphology and morphometry in 7 species (goat, guinea pig, kudu, fox, sheep, flamingo, and duck). Ex situ fibrils had helical morphology and widths of 15-18 nm across all species. Fibril crossover distances had more interspecies variation of 60-130 nm, and species could be grouped based on pitch (twist size). Twisting patterns of in situ fibrils could not be visualized, but in situ widths of 10 nm were measured across all species. In 4 different chicken cases, fibrils differing morphologically from amyloid were consistently detected via both TEM methods, suggesting the possibility of a non-amyloid deposit that is commonly diagnosed as amyloidosis based on its histologic appearance. When available, we recommend routine confirmation of amyloid fibril deposition by TEM.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"429-438"},"PeriodicalIF":1.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11843569/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pyovagina and sepsis due to Streptococcus didelphis in a Virginia opossum. 弗吉尼亚负鼠的脓毒症和脓毒症。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-05-01 Epub Date: 2025-02-12 DOI: 10.1177/10406387251319266

Agustín Rebollada-Merino, Taylor C Chan, Cassandra Guarino, Ryan P Taylor, Gavin R Hitchener

{"title":"Pyovagina and sepsis due to Streptococcus didelphis in a Virginia opossum.","authors":"Agustín Rebollada-Merino, Taylor C Chan, Cassandra Guarino, Ryan P Taylor, Gavin R Hitchener","doi":"10.1177/10406387251319266","DOIUrl":"10.1177/10406387251319266","url":null,"abstract":"Virginia opossums (Didelphis virginiana) are the only native marsupials in the United States of America and Canada. Females have a reproductive tract consisting of a complex duplex (bifurcated) reproductive system, with 2 ovaries, 2 uteri and uterine tubes, and a vaginal complex composed of 2 lateral vaginae, vaginal sinuses, and a urogenital sinus. Few conditions of the reproductive tract have been described in these marsupials. A 2-y-old, female Virginia opossum was presented because of hematuria and was found dead shortly after admission. The autopsy revealed left lateral vaginal torsion and pyovagina. Streptococcus didelphis was isolated and identified from the lateral vaginal exudate, blood, and liver using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Histologic examination confirmed suppurative and lymphoplasmacytic vaginitis, endometritis, cystitis, pyelonephritis and interstitial nephritis, portal hepatitis with fibrosis, myocarditis, and adrenalitis with intralesional gram-positive cocci. These results suggest ascending S. didelphis infection of the lateral vagina with subsequent torsion, bacterial translocation, bacteremia, and sepsis as the cause of death.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"479-481"},"PeriodicalIF":1.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11822774/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143408634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cardiac osteochondrolipoma in a cheetah. 猎豹心脏骨软骨脂肪瘤。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-05-01 Epub Date: 2025-02-28 DOI: 10.1177/10406387251322763

Jeong-Seop Oh, Na-Young Lee, Eun Jung, Ki-Yong Shin, Dong-Hee Chung, Dae-Yong Kim, Soong-Hee Youn

引用次数: 0

Detection of equid alphaherpesvirus 1 in serum samples collected from infected horses. 感染马血清中马甲疱疹病毒1型的检测。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-05-01 Epub Date: 2025-02-28 DOI: 10.1177/10406387251323272

Koji Tsujimura, Hiroshi Bannai, Yoshinori Kambayashi, Manabu Nemoto, Minoru Ohta

{"title":"Detection of equid alphaherpesvirus 1 in serum samples collected from infected horses.","authors":"Koji Tsujimura, Hiroshi Bannai, Yoshinori Kambayashi, Manabu Nemoto, Minoru Ohta","doi":"10.1177/10406387251323272","DOIUrl":"10.1177/10406387251323272","url":null,"abstract":"Equid alphaherpesvirus 1 (EqAHV1; Orthoherpesviridae, Varicellovirus equidalpha1) spreads by viremia to susceptible organs. Because EqAHV1 circulates in the bloodstream in a cell-associated manner, serum samples are not considered valuable for detecting EqAHV1 and have therefore not been tested by highly sensitive detection methods such as real-time PCR (rtPCR). We investigated whether EqAHV1 could be detected by this method in equine serum samples. We performed rtPCR on archived sera and peripheral blood mononuclear cells (PBMCs) collected from 3 horses experimentally inoculated with EqAHV1. Acute-phase field sera from 40 febrile horses, including 11 positive for EqAHV1 on antibody ELISA, were also tested by both standard rtPCR and direct rtPCR without nucleic acid purification. EqAHV1 was detected by standard rtPCR in the PBMCs of the experimentally infected horses for 3-6 d and in the serum of these horses for 5-7 d. Six of the 11 ELISA-positive acute-phase field sera were positive on standard rtPCR, whereas the remaining were negative. All 6 of these samples were positive on direct rtPCR without nucleic acid purification. These results suggest that serum samples can be used to detect EqAHV1; however, false-negatives may result from low viral gene copy numbers.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"495-498"},"PeriodicalIF":1.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11871572/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinicopathologic, molecular, and ultrastructural features of Sarcocystis pinnipedi infection in 2 California sea lions with fatal necrotizing hepatitis. 2例加利福尼亚海狮致死性坏死性肝炎的鳍足肌囊虫感染的临床病理、分子和超微结构特征。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-05-01 Epub Date: 2025-02-20 DOI: 10.1177/10406387251321832

Elliott S Chiu, Devinn M Sinnott, Martha A Delaney, Michael M Garner, Lance Adams, Bill Van Bonn, Kathleen M Colegrove, Katie Haman, Anibal G Armién, Karen Shapiro

{"title":"Clinicopathologic, molecular, and ultrastructural features of Sarcocystis pinnipedi infection in 2 California sea lions with fatal necrotizing hepatitis.","authors":"Elliott S Chiu, Devinn M Sinnott, Martha A Delaney, Michael M Garner, Lance Adams, Bill Van Bonn, Kathleen M Colegrove, Katie Haman, Anibal G Armién, Karen Shapiro","doi":"10.1177/10406387251321832","DOIUrl":"10.1177/10406387251321832","url":null,"abstract":"Sarcocystis pinnipedi is an apicomplexan protozoal parasite that was first recognized during a mass mortality event in juvenile grey seals (Halichoerus grypus) in the northwest Atlantic Ocean. Since its identification, this parasite has been reported in various pinniped species and has been associated with fatal necrotizing hepatitis. Little is known of the host range of S. pinnipedi. Here we report 2 cases of California sea lions (Zalophus californianus) in managed care that died following an 8-d history of inappetence, vomiting, diarrhea, and progressive lethargy with elevated hepatic enzyme activities. Postmortem examination identified hepatitis and icterus. Sarcocystis schizonts and zoites were identified in regions of necrosis. Molecular and ultrastructural findings demonstrated the close relatedness of this Sarcocystis to S. canis, which produces a similar lesion in bears.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"482-488"},"PeriodicalIF":1.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11843571/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-grade astrocytoma in a sheep: clinical, pathology, and genetic investigations. 绵羊的高级别星形细胞瘤：临床、病理和遗传调查。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-05-01 Epub Date: 2025-02-12 DOI: 10.1177/10406387251318694

Sebastian A Mignacca, Timm Konold, Elena Gallisai, Caterina Maestrale, Ana Gomez-Vitores, Alejandro Nunez

{"title":"High-grade astrocytoma in a sheep: clinical, pathology, and genetic investigations.","authors":"Sebastian A Mignacca, Timm Konold, Elena Gallisai, Caterina Maestrale, Ana Gomez-Vitores, Alejandro Nunez","doi":"10.1177/10406387251318694","DOIUrl":"10.1177/10406387251318694","url":null,"abstract":"Primary neoplastic diseases of the CNS in livestock are rare compared to companion animals. Here we describe the clinical, pathologic, and genetic findings in an adult sheep with a high-grade cerebral astrocytoma. Clinically, apathy, ataxia, bilateral amaurosis, and horizontal resting nystagmus were observed. The signs were suggestive of a space-occupying lesion affecting the vestibular and ascending reticular activating systems. The CSF had high total protein, hypercellularity, and pleocytosis. Macroscopically, a friable, gray-pink, ovoid mass (6 × 3 × 4 cm) was observed within most of the right parietal, occipital, and temporal cerebral subcortical areas, extended into the thalamus, hippocampus, and the cerebral peduncles, and reached the ipsilateral choroid plexus and the lateral ventricles. Histologically, the well-delimited, unencapsulated lesion consisted of proliferating neoplastic spindloid-to-polygonal cells mainly arranged in streams and bundles supported by minimal fibrous stroma, and random areas of coagulative necrosis and hemorrhages without pseudopalisading in the center of the mass. Immunohistochemistry revealed strong immunolabeling of spindle cells for S100, vimentin, and glial fibrillary acidic protein. Immunolabeling for neuron-specific enolase was restricted to trapped neurons; oligodendrocyte transcription factor 2 (OLIG2), pan-cytokeratins, and SOX10 were immunonegative. TP53 gene sequencing of the neoplasm did not demonstrate any point mutations involving exons 2-11.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"499-503"},"PeriodicalIF":1.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11822775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143408618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0