Journal of Veterinary Diagnostic Investigation最新文献

{"title":"Development of an indirect ELISA based on the major outer membrane protein for detection of antibodies against <i>Chlamydia felis</i>.","authors":"Haodong Zhang, Rang Wang, Shanshan Dong, Rui Xue, Fengjun Xiang, Xiaolong Li, Dongmei Pan, Xiaoli Yang, Huabo Zhou, Yifeng Qin, Kang Ouyang, Yeshi Yin, Zuzhang Wei, Weijian Huang, Jianming Long, Ying Chen","doi":"10.1177/10406387261485570","DOIUrl":"10.1177/10406387261485570","url":null,"abstract":"<p><p><i>Chlamydia felis</i> is a primary cause of feline conjunctivitis and upper respiratory tract disease. Detection of <i>C. felis</i> largely relies on molecular tests; however, these tests cannot assess prior exposure or herd immunity. In China, no standardized commercial serologic assay for <i>C. felis</i> is available. We developed an indirect ELISA based on the major outer membrane protein (MOMP) of a locally isolated <i>C. felis</i> strain (GXNN36). A truncated <i>ompA</i> gene (encoding MOMP, amino acids 22-392, which retains the major antigenic domains, including the 4 variable domains VD1-4) was expressed in <i>Escherichia coli</i>, and the recombinant protein was purified. After optimizing reaction conditions, the iELISA had high specificity (no cross-reactivity with feline calicivirus, panleukopenia virus, or feline coronavirus), high sensitivity (detection limit up to 1:20,480 dilution), and good reproducibility (intra- and inter-assay CVs <10%). The cutoff optical density (OD450) was 0.630. Assessment against western blotting using 20 clinical sera had 95% concordance. Testing of 144 feline serum samples from Guangxi, China revealed a seroprevalence of 20.1%. Our MOMP-based iELISA using a Chinese <i>C. felis</i> isolate offers a practical and regionally relevant tool for serosurveillance.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387261485570"},"PeriodicalIF":1.4,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13619983/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149069861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PD-L1 immunoreactivity and its association with tumor grade in canine mammary carcinomas.","authors":"Gina P Duarte-Isaza, Gina M Mendez-Callejas, Cruz E Enríquez-Valencia, Darwin A Moreno-Pérez, Carolina Gallego López","doi":"10.1177/10406387261479705","DOIUrl":"10.1177/10406387261479705","url":null,"abstract":"<p><p>Programmed death ligand 1 (PD-L1) is a transmembrane protein involved in tumor immune evasion. Although widely studied in human oncology, its role in canine mammary carcinoma remains poorly characterized. We quantified <i>pd-l1</i> gene mRNA levels by reverse-transcription quantitative real-time PCR (RT-qPCR), evaluated PD-L1 immunoreactivity by immunohistochemistry (IHC) in canine mammary carcinomas, and tested their association with tumor grade and epidemiologic factors using 96 formalin-fixed, paraffin-embedded tissue samples. The mean age of affected dogs was 9.6 y, with Miniature Poodles and mixed-breed dogs most frequently represented. We found no statistically significant associations between <i>pd-l1</i> mRNA levels with histologic subtype or epidemiologic factors. However, PD-L1 immunoreactivity was significantly associated with tumor grade in the bivariate analysis (<i>p =</i> 0.017; Cramer V = 0.3), supporting the diagnostic value of PD-L1 IHC in canine mammary carcinomas and suggesting its utility as a marker of tumor aggressiveness.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387261479705"},"PeriodicalIF":1.4,"publicationDate":"2026-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13612757/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149030544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic and prognostic value of hematology measurands and lactate dehydrogenase isoenzyme activities in canine immune thrombocytopenia at a primary care hospital.","authors":"Shunya Yokota, Takashi Hirano, Takanori Inden, Hiroto Taira, Momoko Narita, Masashi Yuki","doi":"10.1177/10406387261481448","DOIUrl":"10.1177/10406387261481448","url":null,"abstract":"<p><p>Immune thrombocytopenia (ITP) is a common hematologic disorder in dogs for which reliable diagnostic and prognostic markers are limited. We evaluated retrospectively the diagnostic and prognostic value of hematology measurands and lactate dehydrogenase (LDH) isoenzyme activities in a primary care setting. Dogs with thrombocytopenia were classified into ITP (<i>n</i> = 14) and non-ITP (<i>n</i> = 27) groups, and platelet count, plateletcrit, mean platelet volume (MPV), and LDH isoenzyme activity profiles were compared between groups. Platelet count and plateletcrit were significantly lower in the ITP group, with sensitivities and specificities of 92.3% and 100% and 85.7% and 100% at cutoffs of 8 × 10⁹/L and 0.0001 L/L, respectively. LDH1-3 activities were significantly higher, whereas LDH5 activities were significantly lower in the ITP group, although specificity was limited. Within the ITP group, dogs with bleeding (<i>n</i> = 8) had significantly lower MPVs. Dogs responding to prednisolone monotherapy (<i>n</i> = 7) had higher platelet counts and MPVs. The outcome predictive performance of a platelet count ≥3 × 10⁹/L or MPV ≥15.8 fL was excellent (100% sensitivity and specificity in this cohort). Platelet count and plateletcrit were reliable diagnostic indicators for canine ITP in primary care. LDH isoenzyme activities offer limited standalone diagnostic value but may provide supplementary information. MPV may serve as an indicator of bleeding risk and a predictor of treatment response.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387261481448"},"PeriodicalIF":1.4,"publicationDate":"2026-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13612762/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149030876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomarkers for monitoring racehorse musculoskeletal injuries and injury risk.","authors":"Anna M Rauber-Ramos, Emma N Adam, Allen E Page","doi":"10.1177/10406387261478084","DOIUrl":"10.1177/10406387261478084","url":null,"abstract":"<p><p>In most catastrophic racehorse fractures, postmortem examinations have found evidence of chronic accumulation of microdamage attributed to high-stress loading during high-speed exercise. Biological responses to this microdamage accumulation may be an avenue to identify biomarkers indicative of this damage, offering insight into musculoskeletal adaptation and dysregulation. Since the first description of an equine bone degradation biomarker in 1975, our understanding of various biological molecules associated with bone modeling, remodeling, and damage has greatly improved, although none has become a mainstream, clinical screening tool. Here, we describe the history and current state of research on biomarkers in racehorse musculoskeletal health. Although not yet in clinical use, biomarkers present a promising area of research for screening and monitoring racehorses at risk for musculoskeletal injury.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387261478084"},"PeriodicalIF":1.4,"publicationDate":"2026-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13570956/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148956044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methomyl poisoning in dogs: report of 5 cases and literature review.","authors":"Juan Diego Hidalgo-Dittel, Paula Peña-Álvarez, Shirley Calderón-Morera, Steeven Paniagua-Mora, Diego Arias-Alfaro, Diego S Zúñiga-Cortés, Javier Asin, Francisco A Uzal, Nicolas Streitenberger, Laura G Bass, Roberto W I Olivares","doi":"10.1177/10406387261484039","DOIUrl":"10.1177/10406387261484039","url":null,"abstract":"<p><p>Methomyl is a widely available carbamate pesticide that acts as an acetylcholinesterase inhibitor. Here, we describe 5 cases of methomyl poisoning in dogs. The main gross and microscopic findings included pulmonary edema and multisystemic congestion and hemorrhages, which were most severe in the respiratory tract. The diagnosis of poisoning was confirmed in all cases by detecting methomyl in gastric contents using gas chromatography-mass spectrometry. Differential diagnoses include poisoning by organophosphates, coumarin rodenticides, and sodium fluoroacetate, which share clinical and pathology features but differ in pathogenesis. Additionally, we conducted a review of the literature on methomyl poisoning in animals focused on pathogenesis, clinical signs, lesions, and diagnosis.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387261484039"},"PeriodicalIF":1.4,"publicationDate":"2026-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13570966/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148944018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute hepatic necrosis associated with <i>Trema micranthum</i> poisoning in cattle in Brazil.","authors":"Wuglenya D M Silva, Larissa Sabino, Loriany M Gottardi, Jenyffer A O P Rondon, Marcos de A Souza, Marlon Ribeiro, Edson M Colodel, Fabricio Andrade Gomes, Caroline Argenta Pescador, Fernando H Furlan","doi":"10.1177/10406387261484902","DOIUrl":"10.1177/10406387261484902","url":null,"abstract":"<p><p><i>Trema micranthum</i> (L.) Blume (Jamaican nettletree) is a hepatotoxic tree that is widely distributed throughout the Americas and has been associated with poisoning in several domestic animal species. To our knowledge, spontaneous intoxication in cattle has not been reported previously. Here, we describe 2 spontaneous outbreaks of <i>T. micranthum</i> poisoning in Nelore cows in the state of Mato Grosso, Brazil. Affected cattle developed apathy, ataxia, and recumbency, followed by death within hours. Autopsy revealed jaundice and enlarged livers with a diffuse mottled red-and-white appearance. Histologically, severe centrilobular-to-massive hepatocellular coagulative necrosis was observed. Epidemiologic investigation identified recent access to paddocks containing vigorously sprouting <i>T. micranthum</i> following deforestation or tree fall, with clear evidence of grazing. The combination of pathology findings and field investigation established the diagnosis of acute toxic hepatopathy caused by <i>T. micranthum</i>.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387261484902"},"PeriodicalIF":1.4,"publicationDate":"2026-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13570947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148945164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative multiplex detection of 9 feline respiratory pathogens with enhanced sensitivity: a cost-effective integrated platform combining RT-PCR/PCR and capillary electrophoresis.","authors":"Chun-An Chen, Han-Tso Lin, Shi-Wen Goh, John Han-You Lin, Lih-Chiann Wang","doi":"10.1177/10406387261484040","DOIUrl":"10.1177/10406387261484040","url":null,"abstract":"<p><p>Pathogen infections and coinfections are critical determinants of feline upper respiratory tract disease; although the etiologic agents are diverse, their clinical manifestations often overlap. Established detection panels for feline upper respiratory tract infections (FURTIs) primarily focus on core pathogens-feline calicivirus (FCV), felid alphaherpesvirus 1 (FeAHV1), <i>Mycoplasmopsis felis</i> (MF), <i>Chlamydia felis</i> (CF), <i>Bordetella bronchiseptica</i> (BB), and influenza A virus (IAV)-and frequently do not incorporate emerging zoonotic agents (e.g., SARS-CoV-2) and overlooked streptococcal species (<i>Streptococcus equi</i> subsp. <i>zooepidemicus</i> [SEZ] and <i>S. canis</i> [SC]) in a multiplex assay. Furthermore, simultaneous quantification remains technically challenging. We developed an integrated multiplex reverse-transcription PCR (RT-PCR)/PCR-capillary electrophoresis (CE) platform for simultaneous quantitative detection of 9 feline respiratory pathogens (FCV, FeAHV1, MF, CF, BB, IAV, SARS-CoV-2, SEZ, SC). Requiring only a standard thermal cycler and a basic CE instrument, our platform is highly accessible to resource-limited laboratories, offering high specificity and a 1.6-h turnaround time. With a limit of detection of 1 copy/µL across all targets, our multiplex assay had exceptional sensitivity, surpassing conventional singleplex RT-PCR/PCR on both agarose gel and real-time PCR platforms. Notably, we achieved a 2.2-fold higher detection rate than the conventional gold standard (singleplex RT-PCR/PCR with agarose gel). This enhanced sensitivity enabled us to identify even low-pathogen-load samples, often missed by conventional assays. When used with clinical specimens, the platform offered comprehensive coverage of major and emerging zoonotic FURTI pathogens, confirming its potential as a cost-effective, high-efficiency tool for differential diagnosis, targeted therapy, and epidemiologic surveillance.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387261484040"},"PeriodicalIF":1.4,"publicationDate":"2026-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13570967/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148956017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spontaneous metastatic mammary carcinomas in 2 capuchin monkeys: pathology and immunohistochemical findings with a brief literature review.","authors":"Isabel L Macêdo, Liz A Cerqueira, Yasmin N G Fonseca, Antonizete R Souza, Ana L G L Chisté, Márcio B Castro","doi":"10.1177/10406387261479233","DOIUrl":"10.1177/10406387261479233","url":null,"abstract":"<p><p>Spontaneous mammary neoplasms are uncommon in nonhuman primates (NHPs), particularly in neotropical species, and their pathologic aspects and biological behavior remain poorly characterized. Here, we describe the pathology and immunohistochemical features of spontaneous metastatic mammary carcinomas in 2 elderly captive capuchin monkeys (<i>Sapajus libidinosus</i>). A 26-y-old female and a 29-y-old male had unilateral mammary masses and died as a result of complications associated with advanced neoplastic disease. Grossly, both tumors had marked local invasion and systemic metastases involving lymph nodes, lungs, liver, and pericardium. Histologically, both carcinomas were poorly demarcated, infiltrative malignant epithelial proliferations arranged predominantly in solid nests and trabeculae, with central necrosis, marked anisocytosis and anisokaryosis, high mitotic activity, and vascular invasion. Immunohistochemically, both tumors expressed pancytokeratin, CK7, mammaglobin, HER2, and Ki67. The female had a triple-positive phenotype (ER+, PR+, HER2 2+), whereas the male had an HER2-enriched profile (ER low or negative, PR-, HER2 3+). Ki67 indices were high in both cases (31% and 35%, respectively), supporting elevated proliferative activity. The immunophenotypic and pathology findings resembled aggressive high-grade human breast carcinomas associated with poor prognosis and metastatic potential. A literature review confirmed the rarity of spontaneous mammary tumors in neotropical NHPs and highlighted the scarcity of cases with metastases. Collectively, our findings expand the current knowledge of mammary neoplasia in NHPs and reinforce the comparative relevance of these species for understanding similar human breast cancer.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387261479233"},"PeriodicalIF":1.4,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13547549/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148892195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neutrophil myeloperoxidase index in juvenile dogs with parvoviral enteritis.","authors":"Louis C Loubser, Willem J Botha, Yolandi Rautenbach, Anri Celliers","doi":"10.1177/10406387261436360","DOIUrl":"10.1177/10406387261436360","url":null,"abstract":"<p><p>Canine parvovirus is one of the most common causes of infectious enteritis in puppies worldwide. Although various biomarkers have been evaluated to predict prognosis in dogs with parvoviral enteritis (CPE), many are not feasible in routine practice. The neutrophil myeloperoxidase index (MPXI; derived from the Advia 2120 hematology analyzer, Siemens), has shown promise as a marker of inflammation and disease severity in other species. We compared the MPXI in 47 client-owned dogs with established prognostic indicators, including total WBC, neutrophil and lymphocyte count, and serum concentrations of total thyroxine, cortisol, and C-reactive protein (CRP). The MPXI in dogs with CPE did not differ significantly from healthy controls when measured at admission (<i>p</i> = 0.444), at 24 h (<i>p</i> = 0.332), or at 48 h (<i>p</i> = 0.279) after admission. At 24 h after admission, MPXI had a strong positive correlation with serum cortisol (<i>r</i> = 0.87; <i>p</i> < 0.001) and CRP (<i>r</i> = 0.71; <i>p</i> = 0.003) concentrations and a strong negative correlation with WBC (<i>r</i> = -0.82; <i>p</i> < 0.001), neutrophil (<i>r</i> = -0.77; <i>p</i> < 0.001), and lymphocyte (<i>r</i> = -0.90; <i>p</i> < 0.001) counts, as well as serum thyroxine concentration (<i>r</i> = -0.78; <i>p</i> < 0.001). MPXI did not distinguish between diseased and healthy animals. However, increased MPXI in dogs with parvoviral enteritis may indicate the presence of immunoparalysis and be associated with a worse prognosis. Larger prospective studies, including mortality data, are warranted to evaluate MPXI as an accessible and cost-effective prognostic biomarker in CPE.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"936-947"},"PeriodicalIF":1.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13050367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147618999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid identification of African swine fever virus in diagnostic samples using CRISPR-Cas.","authors":"Sekhar Kambakam, Julia Thomas, Suelee Robbe-Austerman, Karthik Shanmuganatham, Rachel Palinski","doi":"10.1177/10406387261432985","DOIUrl":"10.1177/10406387261432985","url":null,"abstract":"<p><p>African swine fever virus (ASFV) is a highly transmissible pathogen affecting swine, causing a devastating disease with high mortality rates in naive populations. Given the likelihood of significant economic impacts associated with an ASF outbreak, considerable resources have been allocated in the United States to safeguard the swine industry against this threat. Ongoing outbreaks of ASF in the Dominican Republic and Haiti further threaten the U.S. swine industry, given their proximity and involvement in movement to and from North America. Although surveillance programs are ongoing, limited point-of-care (POC) tests are available during outbreaks with the sensitivity and specificity standards of laboratory testing (e.g., real-time PCR [rtPCR]). However, the recently developed CRISPR-Cas-based testing systems may offer comparable high-quality results. We sought to develop a low-cost visual detection method for ASFV by employing a recombinase polymerase amplification (RPA)-dependent CRISPR-Cas12a technique that can be utilized in the field as a POC assay. Our CRISPR-Cas12a assay had comparable sensitivity and specificity to rtPCR, both visually and when quantified using a fluorescence reader. In whole blood samples from ASFV-suspect or ASFV-negative cases, our CRISPR assay achieved a sensitivity of 98.3% (10² DNA copies) and a specificity of 100%. Test results of our RPA-CRISPR assay can be visualized in as few as 7 min, with peak fluorescence at 40 min (RPA and CRISPR steps). Our results lay the groundwork for a large-scale POC assay assessment for ASFV detection and offer a robust workflow that works with commonly submitted diagnostic samples.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"926-935"},"PeriodicalIF":1.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13021537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147512871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}