Journal of Veterinary Diagnostic Investigation最新文献_第7页

Emergence of hemorrhagic septicemia caused by MLST type ST64 Pasteurella multocida in a European fallow deer population in Hungary.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-02-14 DOI: 10.1177/10406387251320602

Károly Erdélyi, Krisztina Pintér, Boglárka Pollák, Erika Bakcsa, Levente Szeredi, Pál Lehotzky, Tibor Magyar

{"title":"Emergence of hemorrhagic septicemia caused by MLST type ST64 Pasteurella multocida in a European fallow deer population in Hungary.","authors":"Károly Erdélyi, Krisztina Pintér, Boglárka Pollák, Erika Bakcsa, Levente Szeredi, Pál Lehotzky, Tibor Magyar","doi":"10.1177/10406387251320602","DOIUrl":"10.1177/10406387251320602","url":null,"abstract":"A sudden mass mortality involving 44 fallow deer (Dama dama) occurred in September 2020 at a game management area near Budapest, Hungary. The die-off affected both sexes and all age classes equally. On postmortem examination of 2 subadults and 1 fawn, we found average body condition, diffuse subcutaneous edema, swelling of peripheral lymph nodes, marked hemorrhages in lymph nodes along the esophagus and trachea, and left ventricular dilation. We isolated Pasteurella-like colonies from spleen, liver, small intestine, lung, and brain samples of 7 animals, and identified them as P. multocida serotype B:2 by amplifying kmt1, toxA, and hyaC-hyaD genes, capsular typing by PCR, and serotyping by agar gel diffusion precipitation test, which established the diagnosis of hemorrhagic septicemia as the cause of the outbreak. By further analysis, we determined that all of our P. multocida isolates encoded ptfA, fimA, hsf-2, nanH, hgbA, and pfhA, but we did not detect genes encoding toxA, hsf-1, tbpA, or tadD. We identified our strains by RIRDC MLST as sequence type (ST)122, characteristic for strains causing hemorrhagic septicemia, while using multi-host MLST analysis we assigned these strains to ST64 clustering with P. multocida strains from the 2015 hemorrhagic septicemia outbreak of saiga antelopes in Kazakhstan. We concluded that increased population density and aggregation due to limited drinking water availability played a role in initiating the outbreak.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251320602"},"PeriodicalIF":1.2,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830153/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143425533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pyovagina and sepsis due to Streptococcus didelphis in a Virginia opossum.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-02-12 DOI: 10.1177/10406387251319266

Agustín Rebollada-Merino, Taylor C Chan, Cassandra Guarino, Ryan P Taylor, Gavin R Hitchener

{"title":"Pyovagina and sepsis due to Streptococcus didelphis in a Virginia opossum.","authors":"Agustín Rebollada-Merino, Taylor C Chan, Cassandra Guarino, Ryan P Taylor, Gavin R Hitchener","doi":"10.1177/10406387251319266","DOIUrl":"10.1177/10406387251319266","url":null,"abstract":"Virginia opossums (Didelphis virginiana) are the only native marsupials in the United States of America and Canada. Females have a reproductive tract consisting of a complex duplex (bifurcated) reproductive system, with 2 ovaries, 2 uteri and uterine tubes, and a vaginal complex composed of 2 lateral vaginae, vaginal sinuses, and a urogenital sinus. Few conditions of the reproductive tract have been described in these marsupials. A 2-y-old, female Virginia opossum was presented because of hematuria and was found dead shortly after admission. The autopsy revealed left lateral vaginal torsion and pyovagina. Streptococcus didelphis was isolated and identified from the lateral vaginal exudate, blood, and liver using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Histologic examination confirmed suppurative and lymphoplasmacytic vaginitis, endometritis, cystitis, pyelonephritis and interstitial nephritis, portal hepatitis with fibrosis, myocarditis, and adrenalitis with intralesional gram-positive cocci. These results suggest ascending S. didelphis infection of the lateral vagina with subsequent torsion, bacterial translocation, bacteremia, and sepsis as the cause of death.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251319266"},"PeriodicalIF":1.2,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11822774/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143408634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-grade astrocytoma in a sheep: clinical, pathology, and genetic investigations.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-02-12 DOI: 10.1177/10406387251318694

Sebastian A Mignacca, Timm Konold, Elena Gallisai, Caterina Maestrale, Ana Gomez-Vitores, Alejandro Nunez

{"title":"High-grade astrocytoma in a sheep: clinical, pathology, and genetic investigations.","authors":"Sebastian A Mignacca, Timm Konold, Elena Gallisai, Caterina Maestrale, Ana Gomez-Vitores, Alejandro Nunez","doi":"10.1177/10406387251318694","DOIUrl":"10.1177/10406387251318694","url":null,"abstract":"Primary neoplastic diseases of the CNS in livestock are rare compared to companion animals. Here we describe the clinical, pathologic, and genetic findings in an adult sheep with a high-grade cerebral astrocytoma. Clinically, apathy, ataxia, bilateral amaurosis, and horizontal resting nystagmus were observed. The signs were suggestive of a space-occupying lesion affecting the vestibular and ascending reticular activating systems. The CSF had high total protein, hypercellularity, and pleocytosis. Macroscopically, a friable, gray-pink, ovoid mass (6 × 3 × 4 cm) was observed within most of the right parietal, occipital, and temporal cerebral subcortical areas, extended into the thalamus, hippocampus, and the cerebral peduncles, and reached the ipsilateral choroid plexus and the lateral ventricles. Histologically, the well-delimited, unencapsulated lesion consisted of proliferating neoplastic spindloid-to-polygonal cells mainly arranged in streams and bundles supported by minimal fibrous stroma, and random areas of coagulative necrosis and hemorrhages without pseudopalisading in the center of the mass. Immunohistochemistry revealed strong immunolabeling of spindle cells for S100, vimentin, and glial fibrillary acidic protein. Immunolabeling for neuron-specific enolase was restricted to trapped neurons; oligodendrocyte transcription factor 2 (OLIG2), pan-cytokeratins, and SOX10 were immunonegative. TP53 gene sequencing of the neoplasm did not demonstrate any point mutations involving exons 2-11.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251318694"},"PeriodicalIF":1.2,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11822775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143408618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryptosporidium andersoni-associated proliferative abomasitis in a roan antelope. 一只大羚羊患上了与隐孢子虫-安德森相关的增殖性腹膜炎。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-01-01 Epub Date: 2024-09-25 DOI: 10.1177/10406387241283191

Sai Fingerhood, Justine Shotton, Cecilia Gola, Martha Betson

引用次数: 0

Intra-abdominal nocardiosis and scedosporiosis in a dog: case report and literature review. 一只狗的腹腔线虫病和头孢子虫病：病例报告和文献综述。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-01-01 Epub Date: 2024-10-14 DOI: 10.1177/10406387241287799

Jessica Rose Lambert, Arthur Colombari Cheng, Laura M Lee, Donna Raiford, Emily Zuber, Erin Kilbane, Eric J Fish, Ewa Królak, Katelyn C Hlusko, Maureen McMichael, Rebecca P Wilkes, Nathan P Wiederhold, Connie F Cañete-Gibas, Daniel Felipe Barrantes Murillo

{"title":"Intra-abdominal nocardiosis and scedosporiosis in a dog: case report and literature review.","authors":"Jessica Rose Lambert, Arthur Colombari Cheng, Laura M Lee, Donna Raiford, Emily Zuber, Erin Kilbane, Eric J Fish, Ewa Królak, Katelyn C Hlusko, Maureen McMichael, Rebecca P Wilkes, Nathan P Wiederhold, Connie F Cañete-Gibas, Daniel Felipe Barrantes Murillo","doi":"10.1177/10406387241287799","DOIUrl":"10.1177/10406387241287799","url":null,"abstract":"A 2-y-old, intact female, mixed-breed dog was presented to the veterinary hospital with abdominal distension, anemia, and lethargy following a chronic history of nonspecific gastrointestinal signs. CBC and serum biochemistry revealed moderate nonregenerative anemia with neutrophilia, hypoalbuminemia, hyperglobulinemia, hypoglycemia, decreased urea and creatinine, and hypercholesterolemia. Abdominal radiographs and ultrasound revealed a large heterogeneous mesenteric mass and ascites. Abdominocentesis confirmed septic peritonitis with filamentous bacteria. Fine-needle aspiration of the mass yielded pyogranulomatous inflammation and hyphae. An exploratory laparotomy revealed a large cranial abdominal mass with granulomas present throughout the abdominal cavity. Due to the poor prognosis and disseminated disease, the owner elected euthanasia. Postmortem and histologic examinations detected intralesional mycetomas and bacterial colonies within the mesenteric masses. 16S ribosomal RNA gene PCR and sequencing using formalin-fixed, paraffin-embedded sections identified Nocardia yamanashiensis, Nocardioides cavernae, and Nocardioides zeicaulis. Fungal culture, PCR, and sequencing confirmed Scedosporium apiospermum. Our report highlights the importance of molecular methods in conjunction with culture and histologic findings for diagnosing coinfections caused by infrequent etiologic agents. Additionally, we provide a comprehensive literature review of Scedosporium apiospermum infections in dogs.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"189-198"},"PeriodicalIF":1.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11559917/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142468872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of blood meals from stable flies to evaluate the bovine leukemia virus infection status in cattle herds: a pilot study. 利用厩蝇血餐评估牛群的牛白血病病毒感染状况：一项试点研究。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-01-01 Epub Date: 2024-10-27 DOI: 10.1177/10406387241293081

Kaori Shimizu, Chikahiro Mori, Ayaka Okada, Yasuo Inoshima

{"title":"Use of blood meals from stable flies to evaluate the bovine leukemia virus infection status in cattle herds: a pilot study.","authors":"Kaori Shimizu, Chikahiro Mori, Ayaka Okada, Yasuo Inoshima","doi":"10.1177/10406387241293081","DOIUrl":"10.1177/10406387241293081","url":null,"abstract":"The incidence of enzootic bovine leukosis (EBL), a type of B-cell lymphoma, is increasing in Japan. EBL is caused by bovine leukemia virus (BLV; Retroviridae, Deltaretrovirus bovleu) infection and is diagnosed by detecting antibodies against BLV in milk and blood or BLV DNA in blood. We assessed the feasibility of using stable flies (Stomoxys calcitrans) as a sampling tool to assess BLV infection status in cattle herds. First, we collected blood from 3 cattle herds and, based on the measurement of BLV-proviral load (PVL) by quantitative real-time PCR (qPCR), identified 1) a BLV-free herd, 2) a herd with a low prevalence of BLV-infected cattle and low PVL, and 3) a herd wherein half of the cattle were BLV-infected with low-to-high PVLs. Next, we collected stable flies from the 3 herds, extracted DNA from their blood meals, analyzed it for BLV DNA, and measured the BLV PVL. Cattle DNA and BLV DNA, but not other mammalian DNA, were successfully detected by digestion of the flies. Based on fly blood meal qPCR, we identified one herd as BLV-free and the other 2 herds as having <50% prevalence of BLV-infected cattle with low PVLs. Our fly results were not consistent with preliminary BLV-PVL measurements on cattle blood. Our pilot study indicated that, to assess the feasibility of a stable fly blood meal test as an alternative technique for evaluating BLV infection status in dairy and beef cattle, additional investigations involving more cattle herds and stable flies are needed.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"148-154"},"PeriodicalIF":1.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11559776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Response to the letter to the editor: Coelomitis? 回应致编辑的信：肠炎？

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-01-01 Epub Date: 2024-09-30 DOI: 10.1177/10406387241287515

Daniela Denk

引用次数: 0

Sodium toxicosis in chickens: case series (2014-2023) and literature review. 鸡钠中毒：病例系列（2014-2023）和文献综述。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-01-01 Epub Date: 2024-12-19 DOI: 10.1177/10406387241300476

Carlos Daniel Gornatti-Churria, Robert Poppenga, Carmen F Jerry, Simone T Stoute

{"title":"Sodium toxicosis in chickens: case series (2014-2023) and literature review.","authors":"Carlos Daniel Gornatti-Churria, Robert Poppenga, Carmen F Jerry, Simone T Stoute","doi":"10.1177/10406387241300476","DOIUrl":"10.1177/10406387241300476","url":null,"abstract":"Cases of sodium toxicosis (ST), although reported infrequently, can result in acute morbidity and mortality and extensive losses in affected poultry. We analyzed the clinical, pathologic, and toxicologic findings of 7 diagnosed cases of ST in chicken autopsy submissions at the California Animal Health & Food Safety Laboratory System (CAHFS), University of California-Davis, from 2014 to 2023. We also evaluated the brain sodium concentrations in 10 clinically normal broiler chickens to elucidate potential differences with salt-intoxicated chickens, and reviewed the literature of field cases of ST in chickens and turkeys. Lesions of anasarca described in the 7 ST cases (66 chickens) identified from the CAHFS database included: ascites (62 of 66; 6 of 7 cases); hydropericardium and cardiomegaly (54; 6 of 7); edematous, congested lungs (24; 6 of 7); enlarged, pale kidneys (24; 6 of 7); subcutaneous edema (17; 4 of 7); cystic testes (14; 6 of 7); and cerebral edema (7; 4 of 7). Brain sodium concentrations exceeded 1,800 ppm in only 4 of 24 brains analyzed in our case series. In the feed samples analyzed from 5 ST cases, sodium concentrations exceeded the recommended 2,000 ppm; concentrations detected were 2,500-12,000 ppm. In brains from the 10 clinically normal chickens evaluated, brain sodium concentrations were 1,500-1,700 ppm.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"114-118"},"PeriodicalIF":1.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11656456/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142854678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Performance of 5 commercial ELISA kits for the detection of antibody to bovine leukemia virus. 检测牛白血病病毒抗体的 5 种商业 ELISA 试剂盒的性能。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-01-01 Epub Date: 2024-10-14 DOI: 10.1177/10406387241280629

Vesna Milićević, Dimitrije Glišić, Ljubiša Veljović, Zorana Zurovac Sapundžić, Sofija Šolaja, Nemanja Jezdimirović

{"title":"Performance of 5 commercial ELISA kits for the detection of antibody to bovine leukemia virus.","authors":"Vesna Milićević, Dimitrije Glišić, Ljubiša Veljović, Zorana Zurovac Sapundžić, Sofija Šolaja, Nemanja Jezdimirović","doi":"10.1177/10406387241280629","DOIUrl":"10.1177/10406387241280629","url":null,"abstract":"To prevent significant economic losses, some countries have successfully eradicated enzootic bovine leukosis (EBL), which is caused by bovine leukemia virus (BLV) infection. In Serbia, efforts to eliminate EBL commenced in the late 1990s. Recognizing the disparities in test selection among laboratories and variations in quality, we evaluated the diagnostic sensitivity and specificity of commercial ELISAs using field samples in Serbia. Using 5 commercial ELISA kits, we tested 138 cattle serum samples, submitted for confirmatory testing between 2020 and 2023, along with 100 serum samples from BLV-negative herds. We found 100% agreement of the ID Screen BLV Competition (IDvet), Svanovir BLV gp51-Ab (Svanova), and INgezim BLV Compac 2.0 (Ingenasa) ELISAs. We observed 93% agreement comparing these 3 kits to the Bovine Leukemia Virus Antibody test kit (VMRD). Agreements of 92% and 88.4% were determined between Idexx and IDvet, Svanova, and Ingenasa kits, and between Idexx and VMRD kits, respectively.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"145-147"},"PeriodicalIF":1.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11559717/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142468874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determining diagnostic sensitivity loss limits for sample pooling in duplex rtPCR surveillance testing: Theileria orientalis and Anaplasma marginale. 确定双重 rtPCR 监测测试中样本池的诊断灵敏度损失限度：东方壁虱和边缘疟原虫。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-01-01 Epub Date: 2024-10-26 DOI: 10.1177/10406387241287516

Catharine Burgess, S Michelle Todd, Laura Hungerford, Kevin Lahmers

{"title":"Determining diagnostic sensitivity loss limits for sample pooling in duplex rtPCR surveillance testing: Theileria orientalis and Anaplasma marginale.","authors":"Catharine Burgess, S Michelle Todd, Laura Hungerford, Kevin Lahmers","doi":"10.1177/10406387241287516","DOIUrl":"10.1177/10406387241287516","url":null,"abstract":"To expand surveillance testing capacity through sample pooling, a thorough understanding is needed of how sample dilution through pooling affects the sensitivity of candidate assays. We validated a robust and representative framework for assessing the dilution effect of sample pooling using duplex rtPCR surveillance of Theileria orientalis and Anaplasma marginale, both of which are causative agents of severe anemia in cattle and a serious threat to the cattle industry in Virginia and many other states. We used 200 known-positive samples with Ct values representative of typical surveillance results in a series of pools in which we re-tested each sample individually, followed by each sample diluted in equal volumes with negative samples to make pools of 2, 4, 6, 8, and 10 total samples. We compared the Ct values of the individual positives with the Ct values of each pool size to determine if Ct values increase past the limit of detection in the 45-cycle assay. We observed a maximum of 2% sensitivity loss (no more than 2 of 100 samples returned a false-negative result) for both T. orientalis and A. marginale during the pooling series, with lower-than-expected average Ct increase and sensitivity loss. We conclude that pooling up to 10 samples would be acceptable for regional surveillance of T. orientalis and A. marginale using our rtPCR assay. The described strategy is applicable to validate pooling for a wide range of single and duplex rtPCR assays, which could expand efficient disease surveillance.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"71-78"},"PeriodicalIF":1.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11559834/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0