Journal of Veterinary Diagnostic Investigation最新文献

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An overview of mycotoxicoses in rabbits. 兔霉菌毒素概述
IF 1.2 3区 农林科学
Journal of Veterinary Diagnostic Investigation Pub Date : 2024-09-01 Epub Date: 2024-05-28 DOI: 10.1177/10406387241255945
Theodora K Tsouloufi
{"title":"An overview of mycotoxicoses in rabbits.","authors":"Theodora K Tsouloufi","doi":"10.1177/10406387241255945","DOIUrl":"10.1177/10406387241255945","url":null,"abstract":"<p><p>Mycotoxicoses are usually a consideration in large animal species but can affect companion animals as well. Due to increasing interest and the ease of using rabbits as laboratory models, a growing number of published experimental studies discuss the effects of various mycotoxins on this species. However, the available evidence is fragmented and heterogeneous, and has not recently been collated in a review, to my knowledge. Although mycotoxicoses in rabbits are typically subclinical, clinical signs can include weight loss, anorexia, gastrointestinal disorders, stunted growth, reproductive abnormalities, and susceptibility to infections. An antemortem diagnosis typically relies on a comprehensive clinical history, and assessment of clinical signs and relevant laboratory findings, with confirmation of exposure achieved through the measurement of mycotoxin concentrations in feed or target organs. My review focuses on the clinicopathologic and histopathologic effects of the mycotoxins most important in rabbits, including fumonisins, ochratoxins, aflatoxins, trichothecenes, and zearalenone. This review offers a thorough overview of the effects of mycotoxins in rabbits, serving as a one-stop resource for veterinary practitioners, diagnosticians, and researchers.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11457744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141159365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Wild lagomorphs as potential sources of Francisella tularensis in an urban zoo: a case study. 城市动物园中作为土拉弗氏菌潜在传染源的野生袋鼠:一项案例研究。
IF 1.2 3区 农林科学
Journal of Veterinary Diagnostic Investigation Pub Date : 2024-09-01 Epub Date: 2024-04-23 DOI: 10.1177/10406387241248608
Kelli J Maddock, Caitlin V Rickey, Heidi L Pecoraro
{"title":"Wild lagomorphs as potential sources of <i>Francisella tularensis</i> in an urban zoo: a case study.","authors":"Kelli J Maddock, Caitlin V Rickey, Heidi L Pecoraro","doi":"10.1177/10406387241248608","DOIUrl":"10.1177/10406387241248608","url":null,"abstract":"<p><p>A 9-y-old captive male Pallas' cat (<i>Otocolobus manul</i>) had a 1-mo history of worsening lameness and was euthanized. The animal was submitted to the North Dakota State University-Veterinary Diagnostic Laboratory for autopsy with differential diagnoses of suspected degenerative joint disease or neoplasia. Autopsy revealed icteric tissues and pinpoint foci in the liver, spleen, and all lung lobes. PCR testing was positive for <i>Francisella tularensis</i>, the causative agent of tularemia. Additional cases of tularemia were later identified in wild eastern cottontail rabbits found dead at the same urban zoo. Tularemia has been reported in captive non-human primates and rodent populations with one case linked to wild lagomorph exposure, which was likely the route of exposure in our Pallas' cat case. Tularemia is an occupational risk for zoo staff and laboratorians. Pest management and disease surveillance of wild lagomorph populations in zoos are important preventive measures.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11457743/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140851236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Retrospective review of the pathology of American pikas. 美洲鼠兔病理学回顾。
IF 1.2 3区 农林科学
Journal of Veterinary Diagnostic Investigation Pub Date : 2024-09-01 Epub Date: 2024-05-28 DOI: 10.1177/10406387241256907
Adrienne Barrett, Kali Holder, Susan Knowles, Elise E B LaDouceur
{"title":"Retrospective review of the pathology of American pikas.","authors":"Adrienne Barrett, Kali Holder, Susan Knowles, Elise E B LaDouceur","doi":"10.1177/10406387241256907","DOIUrl":"10.1177/10406387241256907","url":null,"abstract":"<p><p>American pikas (<i>Ochotona princeps</i>) are small lagomorphs that live in mountainous talus areas of western North America. Studies on the histopathology of American pikas are limited. We summarize here the clinical histories, and gross and histologic findings of 12 American pikas, including 9 captive (wild-caught) and 3 wild animals. Death was often attributed to stress (transport, handling, anesthesia) with few-to-no premonitory clinical signs. Infection was the cause of death in 2 cases: 1 had bacterial pyogranulomatous dermatitis, cellulitis, and lymphadenitis with sepsis; the other case had oomycete-induced necrotizing colitis. Incidental parasitic infections included sarcocystosis, nematodosis (oxyurids), and ectoparasitism. Most animals with adequate nutritional status had periportal hepatic lipidosis; this finding was absent in all animals with adipose atrophy, and it is possible that periportal hepatic lipidosis is non-pathologic in American pikas. Three cases had myocardial necrosis that was considered the cause of death; the cause of necrosis was not determined, but it may have been caused by stress or vitamin E-selenium deficiency. Esophageal hyperkeratosis was noted in animals with a history of anorexia and negative energy balance; accumulation of esophageal keratin can result from lack of mucosal abrasion by ingesta. Several histologic findings that are likely normal in American pikas include splenic extramedullary hematopoiesis, thymic tissue in adults, and <i>Clostridium</i> sp. in the enteric lumen.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11529067/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141158426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Retrospective analysis of antimicrobial resistance in bacterial pathogens from pet rabbits in Hong Kong, 2019-2022. 2019-2022年香港宠物兔细菌病原体抗菌药耐药性回顾性分析。
IF 1.2 3区 农林科学
Journal of Veterinary Diagnostic Investigation Pub Date : 2024-09-01 Epub Date: 2024-02-27 DOI: 10.1177/10406387241233546
Chen Xin, Fraser Hill, Ibrahim Elsohaby
{"title":"Retrospective analysis of antimicrobial resistance in bacterial pathogens from pet rabbits in Hong Kong, 2019-2022.","authors":"Chen Xin, Fraser Hill, Ibrahim Elsohaby","doi":"10.1177/10406387241233546","DOIUrl":"10.1177/10406387241233546","url":null,"abstract":"<p><p>Antimicrobial resistance (AMR) is a growing threat to human and animal health. Data are limited on the prevalence of resistant bacteria in pet rabbits. Therefore, we aimed to identify prevalent bacterial infections and AMR profiles among pet rabbits in Hong Kong (HK). Our search of the CityU Veterinary Diagnostic Laboratory (VDL) database found 301 cases of pet rabbits submitted for bacteriologic and antimicrobial susceptibility testing by veterinarians at 20 exotic veterinary clinics across HK between 2019 and 2022. The rabbits were of 8 different breeds and had a median age of 6.5 y, with 54.8% males, 40.2% females, and 5% unspecified. Of the 301 samples received, 168 (55.8%) had positive bacterial growth; 125 (74.4%) had single bacterial isolates, and 43 (25.6%) had mixed cultures. Cultures included <i>Enterococcus faecalis</i> (21.3%) as the most frequently isolated gram-positive bacterium, followed by <i>Streptococcus intermedius</i> (12.5%), and <i>Staphylococcus aureus</i> (11.3%). The most frequently isolated gram-negative bacteria were <i>Pseudomonas aeruginosa</i> (18.1%), followed by <i>Escherichia coli</i> (8.3%), <i>Pasteurella multocida</i> (6.9%), and <i>Klebsiella pneumoniae</i> (4.2%). Approximately 83% of the isolates had acquired resistance to at least one antimicrobial agent, and 49.4% were multidrug-resistant. The isolated bacteria had high levels of resistance to penicillin (69.8%), clindamycin (47.4%), and doxycycline (46.9%). Our findings highlight the high levels of AMR in bacteria isolated from pet rabbit clinical samples in HK; many of these bacteria are zoonotic and pose a public health threat.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11457785/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139983219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Special issue on lagomorph disease investigations. 长尾鼬疾病调查特刊。
IF 1.2 3区 农林科学
Journal of Veterinary Diagnostic Investigation Pub Date : 2024-09-01 Epub Date: 2024-09-12 DOI: 10.1177/10406387241267942
Javier Asin, Fábio Abade Dos Santos, Denise M Imai
{"title":"Special issue on lagomorph disease investigations.","authors":"Javier Asin, Fábio Abade Dos Santos, Denise M Imai","doi":"10.1177/10406387241267942","DOIUrl":"10.1177/10406387241267942","url":null,"abstract":"","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11468601/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142290176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Neoplastic and non-neoplastic lesions in biopsy samples from pet rabbits in Hong Kong: a retrospective analysis, 2019-2022. 2019-2022年香港宠物兔活检样本中的肿瘤性和非肿瘤性病变:回顾性分析。
IF 1.2 3区 农林科学
Journal of Veterinary Diagnostic Investigation Pub Date : 2024-09-01 Epub Date: 2024-07-23 DOI: 10.1177/10406387241261066
Fraser I Hill, May P Y Tse, Andrew D Ferguson, Steve W Mills, Jeanine R Sandy, Charan K Ganta, Ada G Cino-Ozuna, Ibrahim Elsohaby
{"title":"Neoplastic and non-neoplastic lesions in biopsy samples from pet rabbits in Hong Kong: a retrospective analysis, 2019-2022.","authors":"Fraser I Hill, May P Y Tse, Andrew D Ferguson, Steve W Mills, Jeanine R Sandy, Charan K Ganta, Ada G Cino-Ozuna, Ibrahim Elsohaby","doi":"10.1177/10406387241261066","DOIUrl":"10.1177/10406387241261066","url":null,"abstract":"<p><p>Rabbits are popular pets in the urban environment of Hong Kong, ranking third behind cats and dogs. Here we describe the frequency of neoplastic and non-neoplastic lesions in biopsies from pet rabbits submitted to the CityU Veterinary Diagnostic Laboratory between 2019 and 2022, comprising 247 tissue samples from 243 rabbits collected by veterinarians in 19 veterinary clinics. Among the 243 rabbits, there were 128 females (65 spayed), 114 males (54 castrated); sex information was not provided for 1 rabbit. The rabbit breeds included 45 Lionhead, 35 Dwarf, 14 Lop, 11 Dwarf Lop, 5 French Lop, 3 Angora, 2 Dutch, 2 Holland Lop, and 1 each of Netherland Dwarf, Velveteen, Mini Lop, and New Zealand White. The mean ages of rabbits with neoplastic and non-neoplastic lesions were 7.1 and 5.7 y, respectively. The most common neoplastic lesions were adenocarcinoma (26.4%), trichoblastoma (21.4%), sarcoma (9.4%), and thymoma (8.2%). The most common non-neoplastic lesion was uterine cystic endometrial hyperplasia (14.8%), followed by dermal abscess formation in the ventral abdomen or skin of the head (12.5%). Although a broad spectrum of other lesions was described, our findings in biopsies from pet rabbits in Hong Kong are consistent with those in other jurisdictions.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11504112/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141748495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of a peptide-generated antibody to rabbit hemorrhagic disease virus 2 VP60 and its immunohistochemical application in natural cases. 兔出血性疾病病毒 2 VP60 多肽抗体的开发及其在自然病例中的免疫组化应用。
IF 1.2 3区 农林科学
Journal of Veterinary Diagnostic Investigation Pub Date : 2024-09-01 Epub Date: 2024-08-06 DOI: 10.1177/10406387241265370
Alisia A W Weyna, Natalie K Stilwell, Rachel Anders, Brittany McHale, Rita McManamon, Elizabeth W Howerth
{"title":"Development of a peptide-generated antibody to rabbit hemorrhagic disease virus 2 VP60 and its immunohistochemical application in natural cases.","authors":"Alisia A W Weyna, Natalie K Stilwell, Rachel Anders, Brittany McHale, Rita McManamon, Elizabeth W Howerth","doi":"10.1177/10406387241265370","DOIUrl":"10.1177/10406387241265370","url":null,"abstract":"<p><p>Rabbit hemorrhagic disease virus 2 (RHDV2) has spread across the United States infecting and causing death in domestic and wild rabbits. Immunohistochemistry (IHC) would be a useful tool for the detection of RHDV2 antigen in tissues as it is inexpensive and readily achievable in most diagnostic laboratories. However, there is no readily available antibody for this purpose. To fill this void, we generated an RHDV2 capsid protein VP60-specific antibody in chicken eggs and validated the antibody using formalin-fixed tissues from 5 domestic rabbits naturally infected with RHDV2. Viral antigen was detected immunohistochemically in various tissues, most prominently in hepatocytes and macrophages in liver, and in macrophages in spleen and cecal lymphoid tissue. Intravascular mononuclear cells in lung and renal tubular and biliary epithelium also were immunolabeled. Both nuclear and cytoplasmic immunolabeling were observed. This peptide-generated antibody is a potentially useful tool as an adjunct to reverse-transcription PCR or in situ hybridization for detection of RHDV2 in tissues.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11483667/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141897740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Neurodegeneration in a domestic rabbit with severe malnourishment and low hepatic copper. 严重营养不良和低肝铜家兔的神经变性。
IF 1.2 3区 农林科学
Journal of Veterinary Diagnostic Investigation Pub Date : 2024-09-01 Epub Date: 2024-08-05 DOI: 10.1177/10406387241258461
Katherine D Watson, Verena K Affolter, Sara Gardhouse, David Sanchez-Migallon Guzman, Robert H Poppenga, Chai-Fei Li, Kathryn Phillips, Amir Kol, Kevin D Woolard
{"title":"Neurodegeneration in a domestic rabbit with severe malnourishment and low hepatic copper.","authors":"Katherine D Watson, Verena K Affolter, Sara Gardhouse, David Sanchez-Migallon Guzman, Robert H Poppenga, Chai-Fei Li, Kathryn Phillips, Amir Kol, Kevin D Woolard","doi":"10.1177/10406387241258461","DOIUrl":"10.1177/10406387241258461","url":null,"abstract":"<p><p>Copper is a trace element that plays an essential role in neurodevelopment and neurologic function. Acquired copper deficiency has a range of neurologic manifestations, with myelopathy being the most common association. We describe here the clinical, radiologic, histopathologic, and toxicologic findings of a rabbit with malnutrition, neurodegeneration, and suspected copper deficiency. A stray, adult female dwarf rabbit cross (<i>Oryctolagus cuniculus</i>) in poor body condition developed ataxia and pelvic limb weakness progressing to lateral recumbency and urine retention. The clinical findings suggested multifocal brainstem disease with right-sided central vestibular involvement; however, microscopic examination identified thoracic and lumbosacral spinal cord myelopathy. Differentials for the spinal cord changes included neurodegenerative disease, nutritional deficiency, neurotoxin, trauma to the lumbosacral region, and ischemia. Hepatic copper levels were suboptimal at 18 ppm dry weight (RI: 24-150 ppm dry weight). While speculative, copper-deficiency myelopathy is a treatable cause of non-compressive myelopathy that may occur in this species.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11457774/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Orofacial masses in domestic rabbits: a retrospective review of 120 cases from 2 institutions, 2000-2023. 家兔口腔肿块:2000-2023 年两家机构 120 个病例的回顾性研究。
IF 1.2 3区 农林科学
Journal of Veterinary Diagnostic Investigation Pub Date : 2024-09-01 Epub Date: 2024-02-22 DOI: 10.1177/10406387241234326
Emily Rätsep, Latasha Ludwig, Melanie Dobromylskyj
{"title":"Orofacial masses in domestic rabbits: a retrospective review of 120 cases from 2 institutions, 2000-2023.","authors":"Emily Rätsep, Latasha Ludwig, Melanie Dobromylskyj","doi":"10.1177/10406387241234326","DOIUrl":"10.1177/10406387241234326","url":null,"abstract":"<p><p>Orofacial masses or swellings are a common presenting complaint in lagomorphs. Similar gross appearances of the masses can complicate clinical interpretation, and histologic review often provides the final diagnosis. Underlying causes vary from infectious to neoplastic. Although inflammatory changes are most commonly reported, various neoplasms occur, although the prevalence of specific tumor types is relatively unknown. We reviewed retrospectively 120 cases (87.5% biopsy, 12.5% autopsy) of neoplastic and non-neoplastic orofacial masses received from January 2000-February 2023 at 2 institutions: University of Guelph, Canada (Animal Health Laboratory and Department of Pathobiology), and Finn Pathologists, United Kingdom. All final diagnoses were achieved through histologic assessment. We included masses or mass-like swellings from the oral cavity, including the mandible and maxilla, and surrounding skin and soft tissues of the oral cavity and jaw. Submissions included pet and commercial (meat and fur) rabbits. Neoplastic lesions were most common (60%), including trichoblastomas, papillomas, melanocytic neoplasms, sarcomas, round-cell tumors, carcinomas (including squamous cell carcinoma), lipomas, odontogenic neoplasms, polyps, osteoma, neuroma, peripheral keratinizing ameloblastoma, and apocrine adenoma. Inflammatory diagnoses (30%) included abscesses, osteomyelitis, dermatitis, and sialadenitis. Other diagnoses (7%) included cysts, as well as hyperplastic skin and proliferative bone lesions. Three cases had no definitive diagnosis. The importance of histologic assessment in diagnosing orofacial \"masses\" in rabbits is highlighted, given that the most common diagnostic category overall was neoplasia.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11462579/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139931728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antibody response of endangered riparian brush rabbits to vaccination against rabbit hemorrhagic disease virus 2. 濒危河岸刷毛兔对兔出血性疾病病毒 2 疫苗的抗体反应。
IF 1.2 3区 农林科学
Journal of Veterinary Diagnostic Investigation Pub Date : 2024-09-01 Epub Date: 2024-08-23 DOI: 10.1177/10406387241267850
Megan E Moriarty, Jaime L Rudd, Fumika Takahashi, Eric Hopson, Colleen Kinzley, Darren Minier, Alex Herman, Mary Lou Berninger, Fawzi Mohamed, Muzafar Makhdoomi, Leslie W Woods, Hon S Ip, Deana L Clifford
{"title":"Antibody response of endangered riparian brush rabbits to vaccination against rabbit hemorrhagic disease virus 2.","authors":"Megan E Moriarty, Jaime L Rudd, Fumika Takahashi, Eric Hopson, Colleen Kinzley, Darren Minier, Alex Herman, Mary Lou Berninger, Fawzi Mohamed, Muzafar Makhdoomi, Leslie W Woods, Hon S Ip, Deana L Clifford","doi":"10.1177/10406387241267850","DOIUrl":"10.1177/10406387241267850","url":null,"abstract":"<p><p>Rabbit hemorrhagic disease virus 2 (RHDV2; <i>Caliciviridae</i>, <i>Lagovirus europaeus</i>), the cause of a highly transmissible and fatal lagomorph disease, has spread rapidly through the western United States and Mexico, resulting in substantial mortality in domestic and wild rabbits. The disease was first detected in California in May 2020, prompting an interagency/zoo/academia/nonprofit team to implement emergency conservation actions to protect endangered riparian brush rabbits (<i>Sylvilagus bachmani riparius</i>) from RHDV2. Prior to vaccinating wild rabbits, we conducted a vaccine safety trial by giving a single SC dose of Filavac VHD K C+V (Filavie) vaccine to 19 adult wild riparian brush rabbits captured and temporarily held in captivity. Rabbits were monitored for adverse effects, and serum was collected before vaccination, and at 7-10, 14-20, and 60 d post-vaccination. Sera were tested using an ELISA to determine antibody response and timing of seroconversion. Reverse-transcription quantitative real-time PCR (RT-qPCR) was performed on rectal swabs to evaluate infection status. No adverse effects from the vaccine were observed. Before vaccination, 18 of 19 rabbits were seronegative, and RHDV2 was not detected by RT-qPCR on any rectal swabs. After vaccination, all rabbits developed an antibody response, with titers of 1:10-1:160. Seroconversion generally occurred at 7-10 d. The duration of antibody response was ≥60 d in 12 of 13 rabbits. Sixteen animals were released and 4 were recaptured several months later, offering a glimpse into longer duration immune response. Our study has informed vaccination strategies for this species and serves as a model for protecting other vulnerable lagomorphs against RHDV2.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11457773/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142036200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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