Journal of Veterinary Diagnostic Investigation最新文献_第7页

Comparison of digital and optical microscopy for the evaluation of veterinary blood films: a preliminary study. 数字显微镜与光学显微镜用于兽医血膜评价的比较：初步研究。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-09-01 Epub Date: 2026-03-21 DOI: 10.1177/10406387261432653

Stephanie Anderson, Madhumitha Sivakumaran, Trey L DeJong, Mara S Varvil

{"title":"Comparison of digital and optical microscopy for the evaluation of veterinary blood films: a preliminary study.","authors":"Stephanie Anderson, Madhumitha Sivakumaran, Trey L DeJong, Mara S Varvil","doi":"10.1177/10406387261432653","DOIUrl":"10.1177/10406387261432653","url":null,"abstract":"Digital microscopy is increasingly used in veterinary diagnostic pathology. However, limited independent research has been published on its use, especially for the purpose of evaluating blood films. Hence, determining the potential limits of blood film assessments obtained via digital microscopy is needed. We compared the agreement of digital and optical cytology for the detection of common cellular morphology changes and abnormalities in veterinary blood films. Twenty-two veterinary clinical pathologists and residents evaluated canine, feline, and equine blood films on glass slides via optical microscopy and digitized blood film slides, with a ≥8-wk washout period between evaluations. One of the equine cases was a patient experimentally infected with Theileria haneyi. Using a standardized rubric, 16 erythrocyte features, 2 platelet features, and 2 leukocyte features were scored from absent to 4+. Additional comments at pathologist discretion were recorded. Changes in erythrocyte shape, platelets, and leukocytes were readily identified on both digital and glass slides. T. haneyi organisms were identified on significantly fewer digitized blood film slides than glass slides. Additionally, intra-observer consistency was low between digitized blood film slide and glass slide evaluation. Relative to glass slides, digitized blood film slides appear generally adequate for identifying erythrocyte, leukocyte, and platelet morphology changes, but may be inadequate for identifying intracellular T. haneyi organisms; however, more studies are needed. Clinicians should exercise caution when interpreting results from digitized blood film slides in which blood-borne infectious disease may be present.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"948-955"},"PeriodicalIF":1.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13005758/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147491307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Angiocentric T-cell lymphoma in a cat. 猫血管中心性t细胞淋巴瘤。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-09-01 Epub Date: 2026-04-06 DOI: 10.1177/10406387261438148

Kaitlyn H Krebs, Natalia J Strandberg, Francisco R Carvallo-Chaigneau, Mark D Freeman

引用次数: 0

Assessment of the association of ESBL-producing Escherichia coli with clinical severity of chronic enteropathy in dogs in Brazil. 评估产esbl大肠杆菌与巴西犬慢性肠病临床严重程度的关系

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-09-01 Epub Date: 2026-05-14 DOI: 10.1177/10406387261447252

Giovanna Casagrande, Rafael Sartori Flores, Melissa Bossardi, André Felipe Streck, Anelise Bonilla Trindade-Gerardi, Heloísa Theodoro

{"title":"Assessment of the association of ESBL-producing Escherichia coli with clinical severity of chronic enteropathy in dogs in Brazil.","authors":"Giovanna Casagrande, Rafael Sartori Flores, Melissa Bossardi, André Felipe Streck, Anelise Bonilla Trindade-Gerardi, Heloísa Theodoro","doi":"10.1177/10406387261447252","DOIUrl":"10.1177/10406387261447252","url":null,"abstract":"Canine chronic enteropathy (CCE) includes persistent gastrointestinal disorders that may be associated with antimicrobial-resistant bacteria. We evaluated the correlation between clinical severity of CCE and the presence of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in dogs with CCE. We assessed 64 dogs using the canine chronic enteropathy clinical activity index (CCECAI) and analyzed rectal swabs for E. coli and ESBL detection. E. coli was isolated from 28 dogs (44%), with ESBL-positive strains identified in 8 (12%). All ESBL-positive cases had severe clinical scores (CCECAI ≥ 9), with a strong association between ESBL phenotype and disease severity (p < 0.001). These multidrug-resistant isolates remained susceptible to imipenem. Histologic findings confirmed marked intestinal inflammation in ESBL-positive cases. No age or sex association was observed. Our results emphasize the clinical relevance of ESBL-producing E. coli in severe CCE and support the inclusion of microbial profiling in diagnostic and therapeutic strategies.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"1028-1032"},"PeriodicalIF":1.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13179285/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147942215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paramesonephric (Müllerian) tumor in a rabbit initially misidentified as male: clinical, histologic, and molecular findings. 最初被误诊为男性的兔肾旁腺瘤：临床、组织学和分子的发现。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-09-01 Epub Date: 2026-04-27 DOI: 10.1177/10406387261440495

Kenichi Maeda, Kenki Kusumoto, Masamine Takanosu, Yumiko Kagawa, Naomi Kawasaki, Takehiko Kakizaki, Satomi Iwai, Shozo Okano

{"title":"Paramesonephric (Müllerian) tumor in a rabbit initially misidentified as male: clinical, histologic, and molecular findings.","authors":"Kenichi Maeda, Kenki Kusumoto, Masamine Takanosu, Yumiko Kagawa, Naomi Kawasaki, Takehiko Kakizaki, Satomi Iwai, Shozo Okano","doi":"10.1177/10406387261440495","DOIUrl":"10.1177/10406387261440495","url":null,"abstract":"A 5-y-old Holland Lop rabbit, initially presumed male based on a small penis-like structure and pet store information, became anorexic and lethargic. Imaging revealed a large caudal abdominal mass distinct from the urinary bladder and rectum. Exploratory laparotomy revealed the mass was associated with uterine-like structures, which were excised. Histopathology and immunohistochemistry were consistent with a malignant paramesonephric (Müllerian) tumor. PCR analysis of Y-linked genes (SRY, ZFY, RBMY) found none of these genetic markers, suggesting the rabbit was genetically female. No gonadal structures were identified on imaging or at surgery. Despite initial postoperative improvement, the rabbit died 2 wk later as a result of severe rhinitis. This rare case highlights the challenges of identifying reproductive tumors in rabbits, especially when sex is misidentified, and emphasizes the importance of accurate clinical and genetic sex determination for early detection, intervention, and preventive care.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"1111-1114"},"PeriodicalIF":1.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13121224/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147775451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Outbreak of feline tuberculosis caused by Mycobacterium bovis in a German household, a possible domestic zoonosis. 德国一户家庭暴发由牛分枝杆菌引起的猫结核，可能是一种家养人畜共患病。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-09-01 Epub Date: 2026-05-14 DOI: 10.1177/10406387261447251

Hande Peters, Luise Kaspers, Hanka Brangsch, Elisabeth M Liebler-Tenorio, Claudia Bunzenthal, Ann-Kathrin Kühling, Annette Kuczka, Miriam Golestan, Sandra Seifert, Stefanie A Barth

{"title":"Outbreak of feline tuberculosis caused by Mycobacterium bovis in a German household, a possible domestic zoonosis.","authors":"Hande Peters, Luise Kaspers, Hanka Brangsch, Elisabeth M Liebler-Tenorio, Claudia Bunzenthal, Ann-Kathrin Kühling, Annette Kuczka, Miriam Golestan, Sandra Seifert, Stefanie A Barth","doi":"10.1177/10406387261447251","DOIUrl":"10.1177/10406387261447251","url":null,"abstract":"Infections with Mycobacterium bovis can lead to clinical tuberculosis in many mammals. Here, we describe M. bovis infections in 4 of 7 cats from 1 household in Germany. These cats had respiratory disorders at intervals of several months. Two of 4 euthanized animals (cats 3 and 4) were submitted for postmortem examination; lungs failed to collapse and were firm in both cats. In cat 4, a severely enlarged pulmonary lymph node was found, as well as small white granulomas in the spleen, liver, and kidney. Bacterial cultivation identified M. bovis spoligotype SB0120 as the causative pathogen in cats 3 and 4. Molecular genetic fine typing revealed that this genotype had not been reported previously in animals or humans in Germany. Serum from cat 4 shortly before euthanasia, and from cats 5-7, were tested for TB-specific antibodies by ELISA. Cat 4 was strongly positive. In contrast, cats 5-7 were negative and remained negative 4 mo later. Based on our case series, tuberculosis should be considered as a differential diagnosis in pet animals, even in countries that are officially free of the disease. A lack of awareness about tuberculosis could increase the risk of zoonotic infections with M. bovis-for both owners and other animals living in affected households.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"980-990"},"PeriodicalIF":1.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13179299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147942195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Putative congenital bilateral lens anomaly in a free-ranging American black bear cub from North Carolina, USA. 美国北卡罗莱纳自由放养的美国黑熊幼崽的先天性双侧晶状体异常。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-09-01 Epub Date: 2026-03-31 DOI: 10.1177/10406387261418616

C Robert Stilz, K Paige Carmichael, Elizabeth W Howerth, Clinton Barden, Nicole M Nemeth

{"title":"Putative congenital bilateral lens anomaly in a free-ranging American black bear cub from North Carolina, USA.","authors":"C Robert Stilz, K Paige Carmichael, Elizabeth W Howerth, Clinton Barden, Nicole M Nemeth","doi":"10.1177/10406387261418616","DOIUrl":"10.1177/10406387261418616","url":null,"abstract":"An ~6-mo-old, free-ranging, male, American black bear (Ursus americanus) cub that was observed stumbling, acting disoriented, and falling, was dispatched and sent for postmortem evaluation. Bilaterally, round concretions were present in both the anterior and posterior aspects of the lens, disrupting the lens epithelium. These concretions were continuous with and extended from the inner surface of the lens capsule, as seen with a periodic acid-Schiff-hematoxylin reaction. Immunohistochemistry for alpha A crystallin was inconsistent, with weakly positive immunolabeling indicative of lens fibers. Other findings in this cub included emaciation and ursicoptic mange. Based on histopathologic findings, in conjunction with the clinical signs observed prior to death, we suspect that the bilateral lens lesions in this cub caused visual impairment. Congenital ocular defects are rarely reported in free-ranging wildlife. Developmental anomalies affecting the lens have not been previously described in Ursidae, to our knowledge.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"1038-1041"},"PeriodicalIF":1.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13038473/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147581566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occurrence of Trichinella spp. infestations in black bears in Quebec, Canada. 加拿大魁北克省黑熊中旋毛虫感染的发生。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-09-01 Epub Date: 2026-04-09 DOI: 10.1177/10406387261422686

Simon Krückemeier, Christopher Fernandez-Prada, Marie-Odile Benoit-Biancamano

{"title":"Occurrence of Trichinella spp. infestations in black bears in Quebec, Canada.","authors":"Simon Krückemeier, Christopher Fernandez-Prada, Marie-Odile Benoit-Biancamano","doi":"10.1177/10406387261422686","DOIUrl":"10.1177/10406387261422686","url":null,"abstract":"Trichinella spp. are parasitic nematodes with a worldwide distribution that infect mammals, birds, and reptiles. Trichinella is composed of at least 13 taxa; the latest taxon, T13, was identified in wolverines in northwestern Canada in 2020. Trichinella is of public health importance because trichinellosis (trichinosis) is a zoonosis transmitted by eating undercooked meat from wild or domestic animals. In Canada, wild game species are the main source of infection; outbreaks in people originate almost exclusively from consumption of bear and walrus meat. Black bears (Ursus americanus), with a population of ~70,000 individuals, are widespread in Quebec; they are a commonly hunted species and a potential source of infection. To estimate the incidence of this parasite in the black bear population, we collected 250 tongue samples from 14 different Quebec regions. A single sample from a female black bear from the Côte-Nord region was positive, resulting in an estimated prevalence of 0.4% (95% CI [0.01, 2.21]). The larval content of >5 larvae/g in this sample was 5 times the minimal infectious dose for humans. Hence, although the prevalence of Trichinella spp. in our survey of black bears in Quebec was relatively low, clinical manifestation in humans is likely to occur when eating meat from such an infected animal, underscoring the need to follow the recommended cooking practices before consuming bear meat.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"856-862"},"PeriodicalIF":1.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13065637/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147639075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a fluorescence in situ hybridization protocol for use with formalin-fixed paraffin-embedded samples of feline heart, liver, and kidney. 用于福尔马林固定石蜡包埋猫心脏、肝脏和肾脏样品的荧光原位杂交方案的开发。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-09-01 Epub Date: 2026-03-21 DOI: 10.1177/10406387261432648

Matthew J Rolph, Pompei Bolfa, Sarah M Cavanaugh, David Hilchie, Kerry E Rolph

{"title":"Development of a fluorescence in situ hybridization protocol for use with formalin-fixed paraffin-embedded samples of feline heart, liver, and kidney.","authors":"Matthew J Rolph, Pompei Bolfa, Sarah M Cavanaugh, David Hilchie, Kerry E Rolph","doi":"10.1177/10406387261432648","DOIUrl":"10.1177/10406387261432648","url":null,"abstract":"Fluorescence in situ hybridization (FISH) can be used to detect intracellular bacteria in formalin-fixed paraffin-embedded (FFPE) tissues. However, this technique has rarely been applied to samples of feline heart, liver, or kidney to investigate the relationship between intracellular bacteria and chronic inflammation within these organs. Our objective was to develop a robust FISH protocol to detect intracellular bacteria within medium-density FFPE feline tissue samples. Cy3-labeled eubacterial and non-eubacterial probes were applied to samples of monkey intestine, feline intestine, and feline liver, in which bacteria had been visualized on routine histopathology. Although initial tests failed, the addition of pepsin pre-digestion to the test protocol and the adjustment of stringency ensured consistent results. All positive results were confirmed using Gram staining and non-eubacterial probes applied to sequential samples of the 3 tissues. Our FISH protocol using Cy3-labeled eubacterial probes reliably and consistently detected bacteria within FFPE samples of the 3 tissues. The addition of 20-min pepsin pre-digestion and the adjustment of the formamide concentration within the hybridization buffer to 40% were pivotal to the successful use of the protocol. Species-specific probes, along with PCR, culture, and special staining, could be considered to increase sensitivity and specificity of FISH when used for the detection of organisms within tissues.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"873-884"},"PeriodicalIF":1.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13005757/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147491372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A noninferiority trial comparing the sensitivity and specificity of Tritrichomonas foetus RT-rtPCR using 0.9% sterile saline versus PBS as transport medium. 一项非劣效性试验，比较0.9%无菌生理盐水和PBS作为运输介质对毛毛单胞菌胎儿RT-rtPCR的敏感性和特异性。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-09-01 Epub Date: 2026-03-28 DOI: 10.1177/10406387261429063

Tyler M Jumper, Merrilee Thoresen, E Heath King, Duan S Loy, John Dustin Loy, David R Smith

{"title":"A noninferiority trial comparing the sensitivity and specificity of Tritrichomonas foetus RT-rtPCR using 0.9% sterile saline versus PBS as transport medium.","authors":"Tyler M Jumper, Merrilee Thoresen, E Heath King, Duan S Loy, John Dustin Loy, David R Smith","doi":"10.1177/10406387261429063","DOIUrl":"10.1177/10406387261429063","url":null,"abstract":"The reported limit of detection (LOD) for Tritrichomonas foetus reverse-transcription real-time PCR (RT-rtPCR) is one organism/extraction in PBS without prior culture. Our objective was to determine if 0.9% sterile saline (saline) was noninferior to PBS as a transport medium for T. foetus RT-rtPCR testing at the LOD. Each week for 10 wk, preputial washing samples were collected from known T. foetus-negative bulls, centrifuged, and combined into a single pellet that was evenly distributed into stock solutions for both media. The stock solutions were used to prepare sixty 1.3-mL samples of each medium, with 30 samples inoculated with T. foetus to a concentration of 1 organism/100 μL to evaluate sensitivity (Sn) and 30 left uninoculated to evaluate specificity (Sp). We tested 1,200 (SnPBS = 300, Snsaline = 300, SpPBS = 300, Spsaline = 300) samples by RT-rtPCR. Differences in Sn and Sp between media were tested using logistic regression models. The Farrington-Manning test was used to evaluate the difference between parameters with ∆ = 2.5%. For PBS, Sn = 70.7% (95% CI [65.2, 75.8%]) and Sp = 99.7% (95% CI [98.2, 100%]); for saline, Sn = 73.3% (95% CI [68.0, 78.3%]) and Sp = 100% (95% CI [98.8, 100%]). The Spsaline was noninferior to SpPBS (p = 0.002); however, noninferiority of Snsaline to SnPBS was inconclusive (p = 0.08). The Sn for samples inoculated near the LOD in saline was similar to, and Sp was noninferior to, PBS, suggesting that either would be acceptable as a transport medium for T. foetus detection using RT-rtPCR.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"991-996"},"PeriodicalIF":1.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13033042/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147530172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathology of Lankesterella sp. infection in a cliff swallow from California, USA. 美国加州崖燕Lankesterella sp.感染病理。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-09-01 Epub Date: 2026-03-19 DOI: 10.1177/10406387261431781

Connor T Mesler, Michael J Yabsley, Whitney M Kistler, Dan Famini, Francisco A Uzal, Aníbal G Armién, Barbara C Shock, Federico Giannitti

{"title":"Pathology of Lankesterella sp. infection in a cliff swallow from California, USA.","authors":"Connor T Mesler, Michael J Yabsley, Whitney M Kistler, Dan Famini, Francisco A Uzal, Aníbal G Armién, Barbara C Shock, Federico Giannitti","doi":"10.1177/10406387261431781","DOIUrl":"10.1177/10406387261431781","url":null,"abstract":"Lankesterella spp., apicomplexan coccidian protozoa historically associated with amphibians, are increasingly reported in birds. Here, we describe lesions associated with infection from, and depict phylogenetic relationships of, a Lankesterella sp. in a cliff swallow (Petrochelidon pyrrhonota) from California, USA. An 8-wk-old male, hand-raised, cliff swallow housed in an outdoor aviary had progressive nonspecific clinical signs for 12 d. The bird was euthanized because of poor prognosis. Histologically, histiocytic, lymphocytic, and plasmacytic myocarditis and necrotizing pectoral myositis were noted, with intralesional protozoa in macrophages, myocytes, and the interstitium. Mononuclear mural arteritis in the ascending aorta had similar intralesional protozoa. Ovoid-to-spherical, pale-eosinophilic structures, presumed to be meronts, were found within the endothelial cells of the ascending aorta. Sequence analysis of the 18S rRNA gene identified the parasite as a Lankesterella sp., similar to those reported from other Hirundinidae species in Europe. Comorbidities included upper respiratory and conjunctival cryptosporidiosis and vitamin E deficiency, likely contributing to the clinical deterioration. Transmission electron microscopy of the heart revealed intra- and extracellular zoites with ultrastructural features compatible with Lankesterella spp. Most Lankesterella surveys have included healthy birds in Europe; disease is rarely reported. Additional research is needed on the pathogenic potential of Lankesterella infections in birds.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"1048-1054"},"PeriodicalIF":1.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13002475/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147486431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0