一种新的疱疹病毒在蓝企鹅中被认为与心肌变性和坏死有关。

IF 1.2 3区 农林科学 Q3 VETERINARY SCIENCES
Tracie A Seimon, Batya R Nightingale, Martha A Delaney, Robert Moore, Charles E Alex, James F X Wellehan, Denise McAloose
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引用次数: 0

摘要

我们在2只死亡的圈养蓝企鹅(小蓝企鹅)中发现了一种新型疱疹病毒。1只鸟心肌细胞出现中重度心肌细胞萎缩、坏死和嗜酸性核内包涵体(inib);两只鸟均可见网状内皮细胞(RE) INIBs和多灶性RE细胞坏死。组织学结果提示病毒感染。在心肌退行性变的鸟的心肌组织和两只鸟的脾组织中,疱疹病毒PCR检测呈阳性。测序和系统发育分析表明,该病毒作为SpAHV2 (spheniscid alphaherpesvirus 2)加入到Alphaherpesvirinae亚家族中,并在包含Mardivirus、Simplexvirus和variclolovirus属成员的亚分支中形成一个独特的分支点。对另外8例蓝企鹅尸检病例(7例脾脏,1例肝脏)的组织和13只活企鹅结膜-输卵管-直肠联合拭子样本进行疱疹病毒筛查,发现另外5只死企鹅和7只活企鹅的SpAHV2阳性。在7只SpAHV2阳性的死亡企鹅中,有6只存在SpAHV2,并且没有明显的疱疹病毒相关病变导致死亡,这表明该病毒可能是蓝企鹅中的一种地方性病毒,并且复发可能导致疾病和死亡。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A novel herpesvirus in blue penguins putatively associated with myocardial degeneration and necrosis.

We identified a novel herpesvirus in 2 deceased captive blue penguins (Eudyptula minor). Moderate-to-severe myocardiocyte atrophy and necrosis, and eosinophilic intranuclear inclusion bodies (INIBs), were seen in myocardiocytes in one bird; reticuloendothelial (RE) cell INIBs and multifocal RE cell necrosis were seen in both birds. The histologic findings were suggestive of viral infection. A herpesvirus PCR assay was positive in myocardial tissue from the bird with myocardial degeneration and in splenic tissue from both birds. Sequencing and phylogenetic analysis showed that the virus, accessioned as spheniscid alphaherpesvirus 2 (SpAHV2), groups within the Alphaherpesvirinae subfamily and forms a unique branch point in a subclade containing members of the Mardivirus, Simplexvirus, and Varicellovirus genera. Herpesvirus screening of tissues from 8 additional blue penguin postmortem examination cases (7 spleen, 1 liver) and combined conjunctival-choanal-cloacal swab samples from 13 live penguins revealed 5 additional dead and 7 live penguins that were positive for SpAHV2. The presence of SpAHV2 in healthy live animals and lack of significant herpesvirus-associated lesions as the cause of death in 6 of 7 SpAHV2-positive dead penguins suggests that this virus may be an endemic in blue penguins, and that recrudescence may cause disease and death.

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来源期刊
CiteScore
3.00
自引率
6.70%
发文量
127
审稿时长
6-16 weeks
期刊介绍: The Journal of Veterinary Diagnostic Investigation (J Vet Diagn Invest) is an international peer-reviewed journal published bimonthly in English by the American Association of Veterinary Laboratory Diagnosticians (AAVLD). JVDI is devoted to all aspects of veterinary laboratory diagnostic science including the major disciplines of anatomic pathology, bacteriology/mycology, clinical pathology, epidemiology, immunology, laboratory information management, molecular biology, parasitology, public health, toxicology, and virology.
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