Journal of Veterinary Diagnostic Investigation最新文献_第10页

Corrigendum to: Development of a reverse-transcription recombinase polymerase amplification assay with a lateral flow assay for rapid detection of avian orthoavulavirus.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-02-09 DOI: 10.1177/10406387251316943

引用次数: 0

Development of a SYBR Green-based RT-qPCR assay for the rapid detection of peste-des-petits-ruminants virus. 开发基于 SYBR Green 的 RT-qPCR 分析法，用于快速检测反刍兽疫病毒。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-01-27 DOI: 10.1177/10406387241311514

Paravalappil Muraleedharan Arun, Ravindran Rajasekhar, Chintu Ravishankar, Hamza Palekkodan, Sumod Kanjirakkuzhiyil, Shashank Somasekhar

{"title":"Development of a SYBR Green-based RT-qPCR assay for the rapid detection of peste-des-petits-ruminants virus.","authors":"Paravalappil Muraleedharan Arun, Ravindran Rajasekhar, Chintu Ravishankar, Hamza Palekkodan, Sumod Kanjirakkuzhiyil, Shashank Somasekhar","doi":"10.1177/10406387241311514","DOIUrl":"10.1177/10406387241311514","url":null,"abstract":"Peste-des-petits-ruminants (PPR) is primarily a disease of small ruminants caused by peste-des-petits-ruminants virus (PPRV; Paramyxoviridae, Morbillivirus caprinae), formerly the small ruminant morbillivirus. PPRV can cause significant morbidity and mortality in small ruminants and a significant economic impact. Conventional reverse-transcription PCR (RT-PCR), and probe-based and SYBR Green-based RT quantitative real-time PCR (RT-qPCR), are employed for the molecular detection of PPRV. Here we describe a SYBR Green-based RT-qPCR for rapid and sensitive detection of PPRV. We designed the specific primers from the conserved region of the fusion gene (F) of PPRV. The standard curve of the established RT-qPCR assay had a good linear relationship. The developed assay was also 3 log units more sensitive than the conventional RT-PCR, with a detection limit of 13.6 copies and an efficiency of 98.2%. There was no cross-reactivity with other caprine respiratory viruses, namely bluetongue virus, goatpox virus, and orf virus. The positive detection rate of clinical samples was 11 of 64 (17.2%) versus 10 of 64 (15.6%) by conventional RT-PCR. We confirmed our results by sequencing the full F and N genes of the isolates. Our SYBR Green RT-qPCR can be used as a fast, economical, and sensitive alternative to RT-PCR for the detection of PPRV.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"278-283"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773506/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mandibular ossifying fibroma in 2 cattle in Brazil. 巴西2头牛的下颌骨化纤维瘤。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-01-27 DOI: 10.1177/10406387241313449

João P da S Cavasani, Ícaro G Dos Santos, Daniela M Garcia, Camilo E B Olaya, Adriele A de Queiroz, Pedro E B Néspoli, Marcos de A Souza, Edson M Colodel

{"title":"Mandibular ossifying fibroma in 2 cattle in Brazil.","authors":"João P da S Cavasani, Ícaro G Dos Santos, Daniela M Garcia, Camilo E B Olaya, Adriele A de Queiroz, Pedro E B Néspoli, Marcos de A Souza, Edson M Colodel","doi":"10.1177/10406387241313449","DOIUrl":"10.1177/10406387241313449","url":null,"abstract":"Ossifying fibroma (OF) is a slow-growing, expansive, and benign fibro-osseous neoplasm that is rare in cattle. It mainly affects the craniofacial bones, especially the mandible. Here, we report 2 cases of mandibular OF in Nelore and mixed-breed steers with enlarged masses in the rostral portion of the mandible. Radiographic analysis of case 1 revealed an oval, lobed mass with the radiopacity of bone tissue that displaced the incisors laterodorsally. Histologically, both masses were composed of a proliferation of spindloid-to-stellate cells, supported by a dense fibrovascular stroma, with bony trabeculae surrounded by stroma and covered by a single layer of osteoblasts. Cellular pleomorphism was low, anisocytosis and anisokaryosis were negligible, and mitotic figures were not observed. The clinical, radiologic, gross, and histologic changes are compatible with OF. The primary differential diagnoses of OF are fibrous dysplasia and osteoma; their differentiation can be difficult. Fibroma, low-grade osteosarcoma, and multilobular sarcoma of bone are also differential diagnoses, with striking features that facilitate their exclusion.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"367-370"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773502/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of blood meals from stable flies to evaluate the bovine leukemia virus infection status in cattle herds: a pilot study. 利用厩蝇血餐评估牛群的牛白血病病毒感染状况：一项试点研究。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-01-01 Epub Date: 2024-10-27 DOI: 10.1177/10406387241293081

Kaori Shimizu, Chikahiro Mori, Ayaka Okada, Yasuo Inoshima

{"title":"Use of blood meals from stable flies to evaluate the bovine leukemia virus infection status in cattle herds: a pilot study.","authors":"Kaori Shimizu, Chikahiro Mori, Ayaka Okada, Yasuo Inoshima","doi":"10.1177/10406387241293081","DOIUrl":"10.1177/10406387241293081","url":null,"abstract":"The incidence of enzootic bovine leukosis (EBL), a type of B-cell lymphoma, is increasing in Japan. EBL is caused by bovine leukemia virus (BLV; Retroviridae, Deltaretrovirus bovleu) infection and is diagnosed by detecting antibodies against BLV in milk and blood or BLV DNA in blood. We assessed the feasibility of using stable flies (Stomoxys calcitrans) as a sampling tool to assess BLV infection status in cattle herds. First, we collected blood from 3 cattle herds and, based on the measurement of BLV-proviral load (PVL) by quantitative real-time PCR (qPCR), identified 1) a BLV-free herd, 2) a herd with a low prevalence of BLV-infected cattle and low PVL, and 3) a herd wherein half of the cattle were BLV-infected with low-to-high PVLs. Next, we collected stable flies from the 3 herds, extracted DNA from their blood meals, analyzed it for BLV DNA, and measured the BLV PVL. Cattle DNA and BLV DNA, but not other mammalian DNA, were successfully detected by digestion of the flies. Based on fly blood meal qPCR, we identified one herd as BLV-free and the other 2 herds as having <50% prevalence of BLV-infected cattle with low PVLs. Our fly results were not consistent with preliminary BLV-PVL measurements on cattle blood. Our pilot study indicated that, to assess the feasibility of a stable fly blood meal test as an alternative technique for evaluating BLV infection status in dairy and beef cattle, additional investigations involving more cattle herds and stable flies are needed.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"148-154"},"PeriodicalIF":1.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11559776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intra-abdominal nocardiosis and scedosporiosis in a dog: case report and literature review. 一只狗的腹腔线虫病和头孢子虫病：病例报告和文献综述。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-01-01 Epub Date: 2024-10-14 DOI: 10.1177/10406387241287799

Jessica Rose Lambert, Arthur Colombari Cheng, Laura M Lee, Donna Raiford, Emily Zuber, Erin Kilbane, Eric J Fish, Ewa Królak, Katelyn C Hlusko, Maureen McMichael, Rebecca P Wilkes, Nathan P Wiederhold, Connie F Cañete-Gibas, Daniel Felipe Barrantes Murillo

{"title":"Intra-abdominal nocardiosis and scedosporiosis in a dog: case report and literature review.","authors":"Jessica Rose Lambert, Arthur Colombari Cheng, Laura M Lee, Donna Raiford, Emily Zuber, Erin Kilbane, Eric J Fish, Ewa Królak, Katelyn C Hlusko, Maureen McMichael, Rebecca P Wilkes, Nathan P Wiederhold, Connie F Cañete-Gibas, Daniel Felipe Barrantes Murillo","doi":"10.1177/10406387241287799","DOIUrl":"10.1177/10406387241287799","url":null,"abstract":"A 2-y-old, intact female, mixed-breed dog was presented to the veterinary hospital with abdominal distension, anemia, and lethargy following a chronic history of nonspecific gastrointestinal signs. CBC and serum biochemistry revealed moderate nonregenerative anemia with neutrophilia, hypoalbuminemia, hyperglobulinemia, hypoglycemia, decreased urea and creatinine, and hypercholesterolemia. Abdominal radiographs and ultrasound revealed a large heterogeneous mesenteric mass and ascites. Abdominocentesis confirmed septic peritonitis with filamentous bacteria. Fine-needle aspiration of the mass yielded pyogranulomatous inflammation and hyphae. An exploratory laparotomy revealed a large cranial abdominal mass with granulomas present throughout the abdominal cavity. Due to the poor prognosis and disseminated disease, the owner elected euthanasia. Postmortem and histologic examinations detected intralesional mycetomas and bacterial colonies within the mesenteric masses. 16S ribosomal RNA gene PCR and sequencing using formalin-fixed, paraffin-embedded sections identified Nocardia yamanashiensis, Nocardioides cavernae, and Nocardioides zeicaulis. Fungal culture, PCR, and sequencing confirmed Scedosporium apiospermum. Our report highlights the importance of molecular methods in conjunction with culture and histologic findings for diagnosing coinfections caused by infrequent etiologic agents. Additionally, we provide a comprehensive literature review of Scedosporium apiospermum infections in dogs.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"189-198"},"PeriodicalIF":1.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11559917/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142468872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryptosporidium andersoni-associated proliferative abomasitis in a roan antelope. 一只大羚羊患上了与隐孢子虫-安德森相关的增殖性腹膜炎。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-01-01 Epub Date: 2024-09-25 DOI: 10.1177/10406387241283191

Sai Fingerhood, Justine Shotton, Cecilia Gola, Martha Betson

引用次数: 0

Response to the letter to the editor: Coelomitis? 回应致编辑的信：肠炎？

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-01-01 Epub Date: 2024-09-30 DOI: 10.1177/10406387241287515

Daniela Denk

引用次数: 0

Sodium toxicosis in chickens: case series (2014-2023) and literature review. 鸡钠中毒：病例系列（2014-2023）和文献综述。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-01-01 Epub Date: 2024-12-19 DOI: 10.1177/10406387241300476

Carlos Daniel Gornatti-Churria, Robert Poppenga, Carmen F Jerry, Simone T Stoute

{"title":"Sodium toxicosis in chickens: case series (2014-2023) and literature review.","authors":"Carlos Daniel Gornatti-Churria, Robert Poppenga, Carmen F Jerry, Simone T Stoute","doi":"10.1177/10406387241300476","DOIUrl":"10.1177/10406387241300476","url":null,"abstract":"Cases of sodium toxicosis (ST), although reported infrequently, can result in acute morbidity and mortality and extensive losses in affected poultry. We analyzed the clinical, pathologic, and toxicologic findings of 7 diagnosed cases of ST in chicken autopsy submissions at the California Animal Health & Food Safety Laboratory System (CAHFS), University of California-Davis, from 2014 to 2023. We also evaluated the brain sodium concentrations in 10 clinically normal broiler chickens to elucidate potential differences with salt-intoxicated chickens, and reviewed the literature of field cases of ST in chickens and turkeys. Lesions of anasarca described in the 7 ST cases (66 chickens) identified from the CAHFS database included: ascites (62 of 66; 6 of 7 cases); hydropericardium and cardiomegaly (54; 6 of 7); edematous, congested lungs (24; 6 of 7); enlarged, pale kidneys (24; 6 of 7); subcutaneous edema (17; 4 of 7); cystic testes (14; 6 of 7); and cerebral edema (7; 4 of 7). Brain sodium concentrations exceeded 1,800 ppm in only 4 of 24 brains analyzed in our case series. In the feed samples analyzed from 5 ST cases, sodium concentrations exceeded the recommended 2,000 ppm; concentrations detected were 2,500-12,000 ppm. In brains from the 10 clinically normal chickens evaluated, brain sodium concentrations were 1,500-1,700 ppm.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"114-118"},"PeriodicalIF":1.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11656456/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142854678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Performance of 5 commercial ELISA kits for the detection of antibody to bovine leukemia virus. 检测牛白血病病毒抗体的 5 种商业 ELISA 试剂盒的性能。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-01-01 Epub Date: 2024-10-14 DOI: 10.1177/10406387241280629

Vesna Milićević, Dimitrije Glišić, Ljubiša Veljović, Zorana Zurovac Sapundžić, Sofija Šolaja, Nemanja Jezdimirović

{"title":"Performance of 5 commercial ELISA kits for the detection of antibody to bovine leukemia virus.","authors":"Vesna Milićević, Dimitrije Glišić, Ljubiša Veljović, Zorana Zurovac Sapundžić, Sofija Šolaja, Nemanja Jezdimirović","doi":"10.1177/10406387241280629","DOIUrl":"10.1177/10406387241280629","url":null,"abstract":"To prevent significant economic losses, some countries have successfully eradicated enzootic bovine leukosis (EBL), which is caused by bovine leukemia virus (BLV) infection. In Serbia, efforts to eliminate EBL commenced in the late 1990s. Recognizing the disparities in test selection among laboratories and variations in quality, we evaluated the diagnostic sensitivity and specificity of commercial ELISAs using field samples in Serbia. Using 5 commercial ELISA kits, we tested 138 cattle serum samples, submitted for confirmatory testing between 2020 and 2023, along with 100 serum samples from BLV-negative herds. We found 100% agreement of the ID Screen BLV Competition (IDvet), Svanovir BLV gp51-Ab (Svanova), and INgezim BLV Compac 2.0 (Ingenasa) ELISAs. We observed 93% agreement comparing these 3 kits to the Bovine Leukemia Virus Antibody test kit (VMRD). Agreements of 92% and 88.4% were determined between Idexx and IDvet, Svanova, and Ingenasa kits, and between Idexx and VMRD kits, respectively.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"145-147"},"PeriodicalIF":1.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11559717/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142468874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A retrospective study of lingual lesions in 793 dogs and 406 cats at the Athens Veterinary Diagnostic Laboratory, 2010-2020. 2010-2020 年雅典兽医诊断实验室对 793 只狗和 406 只猫的舌病变进行的回顾性研究。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-01-01 Epub Date: 2024-09-12 DOI: 10.1177/10406387241278888

Jesse Riker, Daniel R Rissi

{"title":"A retrospective study of lingual lesions in 793 dogs and 406 cats at the Athens Veterinary Diagnostic Laboratory, 2010-2020.","authors":"Jesse Riker, Daniel R Rissi","doi":"10.1177/10406387241278888","DOIUrl":"10.1177/10406387241278888","url":null,"abstract":"Lingual biopsies are a common type of sample submission at the Athens Veterinary Diagnostic Laboratory (AVDL). Here we describe the pathology diagnoses of 793 canine and 406 feline lingual biopsies submitted to the AVDL in a 10-y period. Non-neoplastic lesions accounted for 450 diagnoses (57%) in dogs and 239 diagnoses (59%) in cats. Canine non-neoplastic lesions consisted of inflammatory lesions (286 cases; 64% of non-neoplastic lesions) and tumor-like proliferative lesions (164 cases; 36% of non-neoplastic lesions). Feline non-neoplastic lesions consisted of inflammatory lesions (228 cases; 95% of non-neoplastic lesions) and tumor-like proliferative lesions (11 cases; 5% of non-neoplastic lesions). The most common canine neoplasms were melanocytic neoplasms (103 cases; 30% of neoplasms) and epithelial neoplasms (102 cases; 30% of neoplasms), followed by mesenchymal neoplasms (90 cases; 26% of neoplasms) and round cell neoplasms (48 cases; 14% of neoplasms). Approximately 43% of melanocytic neoplasms affected Chow Chows and Labrador Retrievers, and 20% of epithelial neoplasms affected Labrador Retrievers. In cats, most tumors were epithelial (158 cases; 94% of neoplasms), followed by mesenchymal (8 cases; 5% of neoplasms) and round cell neoplasms (1 case; 1% of neoplasms). Over 50% of neoplasms of cats affected domestic shorthair cats. Although the percentage of lingual biopsies that had a neoplastic diagnosis was roughly the same between species, the diversity of neoplasms was much greater in dogs than in cats.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"176-183"},"PeriodicalIF":1.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11559754/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142290169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0