Daniel Felipe Barrantes Murillo, Russell C Cattley, John M Cullen, Cornelius Withers, Jordan Towns, Rachel Pfeifle, Anne Wooldridge, Rachel L A L T Neto
{"title":"Intrahepatic mucinous cholangiocarcinoma with recurrent colic in a horse case report and literature review of cholangiocarcinoma in horses.","authors":"Daniel Felipe Barrantes Murillo, Russell C Cattley, John M Cullen, Cornelius Withers, Jordan Towns, Rachel Pfeifle, Anne Wooldridge, Rachel L A L T Neto","doi":"10.1177/10406387241245775","DOIUrl":"10.1177/10406387241245775","url":null,"abstract":"<p><p>A 17-y-old Arabian mare was presented to the Auburn Large Animal Veterinary Teaching Hospital with a long-term history of intermittent mild recurrent colic that responded to medical treatment. CBC revealed mild lymphopenia; serum biochemistry findings were of increased gamma-glutamyl transferase and creatine kinase activities, hyperferremia, hyperglycemia, hypomagnesemia, and hypokalemia. Abdominocentesis was compatible with low-protein transudate. Due to the progression and duration of clinical signs, the owner elected euthanasia. Postmortem examination and histopathology confirmed a cholangiocarcinoma. The neoplastic cells were arranged in large cysts containing lakes of mucin that comprised 90% of the tumor volume; thus, a mucinous variant was determined. The neoplastic cells had strong cytoplasmic immunolabeling for cytokeratin 19 and lacked immunolabeling for hepatocyte paraffin 1, supporting bile duct origin. Cholangiocarcinomas are infrequent tumors in horses with nonspecific and slow progressive clinical signs, including recurrent colic. Mucinous cholangiocarcinomas are seldom reported in veterinary medicine and, to our knowledge, have not been reported previously in horses.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11185110/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140856505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Agustín Rebollada-Merino, Carmen Bárcena, Néstor Porras, Lucas Domínguez, Antonio Rodríguez-Bertos
{"title":"Forensic findings in urban red fox mortality in the metropolitan area of Madrid, 2014-2022.","authors":"Agustín Rebollada-Merino, Carmen Bárcena, Néstor Porras, Lucas Domínguez, Antonio Rodríguez-Bertos","doi":"10.1177/10406387241239921","DOIUrl":"10.1177/10406387241239921","url":null,"abstract":"<p><p>The expanding presence of red foxes (<i>Vulpes vulpes</i>) in urban and suburban regions could potentially lead to increased instances of human aggression towards this species. We studied 10 deceased red foxes that were submitted by law enforcement agencies in the metropolitan area of Madrid in 2014-2022 because of suspected abuse. Forensic autopsies were performed to establish the cause and manner of death. In 4 of the 10 cases, the cause of death was deemed unnatural, involving blunt-force trauma (<i>n</i> = 2), asphyxia resulting from hanging (<i>n</i> = 1), and firearm injury (<i>n</i> = 1). Among the remaining cases, most had succumbed to natural causes (<i>n</i> = 4), often marked by severe emaciation and a high burden of parasites, primarily <i>Sarcoptes scabiei</i>. In 2 cases, death was undetermined given the poor preservation of the carcass. The growing prevalence of wildlife species in urban areas, particularly red foxes, may require forensic veterinary investigation of deaths potentially related to abuse.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11185108/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140859629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Congenital localized cutaneous Langerhans cell histiocytosis in a Holstein calf.","authors":"Jørgen S Agerholm, Gary Mason, David Steffen","doi":"10.1177/10406387241245778","DOIUrl":"10.1177/10406387241245778","url":null,"abstract":"<p><p>Distinct solitary dermal nodules, either covered by an alopecic, or sometimes ulcerated, epidermis, were noticed on the head of a stillborn Holstein calf. The head was submitted for autopsy, and the nodules were found to consist of homogeneous, diffuse pale-yellow, soft-tissue masses with distinct margins that elevated the epidermis above the adjacent skin. Histologically, the dermal nodules were well-delineated on the deep margin approaching the cutaneous muscle and consisted of perivascular neoplastic infiltrates of round cells that in some places coalesced into sheets that extended into the dermis and subcutis. Neoplastic cells separated adnexa and collagen. Immunohistochemistry revealed intense tumor cell expression of vimentin, Iba1, E-cadherin, and CD204; expression of CD18 was faint. The masses were diagnosed as Langerhans cell histiocytosis. Congenital cutaneous Langerhans cell histiocytosis has not been reported previously in cattle, to our knowledge, and should be included in the differential diagnosis of congenital nodular skin lesions.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11185123/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140864473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cholangiocarcinoma with carcinomatosis in a sugar glider.","authors":"Alyssa L Williams, Fabio Brum Rosa","doi":"10.1177/10406387241252815","DOIUrl":"10.1177/10406387241252815","url":null,"abstract":"<p><p>A 7-y-old, castrated male, leucistic sugar glider (<i>Petaurus breviceps</i>) was presented because of a progressive history of lethargy, ataxia, diarrhea, and anorexia. Abdominal ultrasound revealed fluid in the abdomen and an infiltrative mass in the liver. Due to a poor prognosis, euthanasia was performed. Postmortem examination revealed a focally extensive, infiltrative, off-white, firm mass in the liver with adhesion to the omentum, mesentery, gastric serosa, and diaphragm. The remaining hepatic parenchyma was diffusely yellow. Histologically, the hepatic mass was consistent with metastatic cholangiocarcinoma (cholangiocellular carcinoma) with proliferation of neoplastic epithelial cells surrounded by marked desmoplasia. Neoplastic cells expanded and infiltrated the adjacent omentum, mesentery, and the serosal surfaces of the stomach, kidney, and small and large intestines. To our knowledge, cholangiocarcinoma has not been reported previously in a sugar glider.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11185111/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140898928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Catherine Wilson, Steven J Philp, Katherine Hughes
{"title":"Torsion of the caudate lobe of the liver and concurrent necrohemorrhagic typhlocolitis in a zoo-housed Patagonian mara.","authors":"Catherine Wilson, Steven J Philp, Katherine Hughes","doi":"10.1177/10406387241248594","DOIUrl":"10.1177/10406387241248594","url":null,"abstract":"<p><p>Liver lobe torsion has been reported in many species, with frequent reports in rabbits. Here we describe caudate liver lobe torsion and concurrent necrohemorrhagic typhlocolitis in a Patagonian mara (syn: Patagonian cavy, Patagonian hare, <i>Dolichotis patagonum</i>). Following acute death, postmortem examination findings included torsion of the hepatic caudate process, which had fibrous adhesions to the pancreas indicating chronicity. The cecal apex and proximal 30 cm of colon had regionally reddened serosa and diffusely roughened and reddened mucosa with brown-red and granular luminal contents. Key histologic findings included massive necrosis of the torsed hepatic caudate lobe, consistent with infarction, necrotizing hepatitis in remaining areas of liver, necrohemorrhagic typhlocolitis, adrenocortical necrosis and hemorrhage, and renal tubular degeneration and necrosis with tubular casts. Bacterial culture of cecal contents yielded pure growth of <i>Salmonella</i> spp. Death was attributed to toxemia or bacteremia resulting from <i>Salmonella</i> spp. infection, as the hepatic lobe torsion appeared chronic. It was undetermined if the liver lobe torsion predisposed to gastrointestinal compromise and infection. Patagonian maras have some anatomical similarities to rabbits and are highly cursorial, not dissimilar to hares, <i>Lepus</i> spp. We speculate that these characteristics may increase the likelihood of hepatic caudate lobe torsion in this species.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11185122/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140870514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cutaneous plasmacytoma with Mott cell differentiation in a dog.","authors":"Cheryl L Auch, Alyona Michael","doi":"10.1177/10406387241251840","DOIUrl":"10.1177/10406387241251840","url":null,"abstract":"<p><p>Cytologic evaluation of aspirate slides from a small, <1-cm, interdigital mass on a 9-y-old, spayed female Yorkshire Terrier revealed a proliferation of discrete, round cells containing few-to-many, variably sized, round, eosinophilic, cytoplasmic inclusions. The top differentials based on the cytologic findings were either a plasma cell tumor or a B-cell lymphoma with Mott cell differentiation. The unencapsulated, well-demarcated, multilobulated round-cell neoplasm was completely excised. Immunohistochemical stains were performed to further characterize the neoplasm, which had immunolabeling for multiple myeloma oncogene 1 and vimentin, but did not react with CD3, CD20, melan A, or ionized calcium-binding adapter molecule 1, nor with a Giemsa special stain. Ultrastructurally, the cytoplasmic granules had Russell body-like morphology. A solitary, cutaneous plasmacytoma with Mott cell differentiation has not been described previously in veterinary medicine, to our knowledge.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11185116/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141260566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"RNAscope: a novel method for the detection of <i>Heterobilharzia americana</i> ova in canine liver.","authors":"Priscilla A Devora, Andrea N Johnston","doi":"10.1177/10406387241241329","DOIUrl":"10.1177/10406387241241329","url":null,"abstract":"<p><p>Canine schistosomiasis caused by <i>Heterobilharzia americana</i> can lead to severe morbidity and eventual mortality, in part due to the deposition of fluke ova in the liver and gastrointestinal tract, which promotes an influx of peri-ova inflammatory cells. Although fluke eggs can be identified in H&E-stained histologic sections, cases exist in which only fragments of the ova persist, or the egg is obscured by inflammatory infiltrates, which can confound definitive histologic diagnosis. Unfortunately, antibodies specific to <i>Heterobilharzia</i> are not commercially available for immunohistochemical labeling. Therefore, we aimed to use an RNA in situ hybridization strategy to fluorescently label <i>Heterobilharzia</i> ova. Using the <i>H. americana</i> 18S rRNA sequence, we developed an RNA probe and validated its performance on archival formalin-fixed, paraffin-embedded canine tissue. A positive signal was observed for all identifiable ova, fragmented and whole. Use of this methodology could aid understanding of the pathogenesis of <i>H. americana</i> infection in dogs. This technique augments standard diagnostic methodology, enabling spatial colocalization of fluke ova and inflammatory infiltrates when using fluorescent techniques.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11185124/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140194083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marcia R S Ilha, Katie A Dawson, Emily L Atkinson, Erin A Graham, Moges W Woldemeskel, Yung-Yi C Mosley, Michele D Coarsey, Hemant K Naikare
{"title":"Retrospective study of laboratory-based surveillance of rabies in wild and domestic animals in the southern United States, 2010-2021.","authors":"Marcia R S Ilha, Katie A Dawson, Emily L Atkinson, Erin A Graham, Moges W Woldemeskel, Yung-Yi C Mosley, Michele D Coarsey, Hemant K Naikare","doi":"10.1177/10406387241247283","DOIUrl":"10.1177/10406387241247283","url":null,"abstract":"<p><p>We performed a retrospective study of all case submissions for the rabies virus (RABV) direct fluorescent antibody test (DFAT) requested of the Tifton Veterinary Diagnostic and Investigational Laboratory (Tifton, GA, USA) between July 2010 and June 2021. Submitted were 792 samples from 23 animal species from 89 counties in Georgia, and 4 neighboring counties in Florida, 1 in South Carolina, and 1 in Alabama. In 13 (1.6%) cases, the DFAT result was inconclusive; 779 (98.4%) cases had a conclusive (positive or negative) test result. Of these 779 cases, 79 (10.1%) tested positive across 10 species. The remaining 700 (89.9%) cases were negative. The main reason for submission for RABV testing was human exposure to a potentially rabid animal in 414 (52.3%) cases. Among the 79 positive cases, 74 (93.7%) involved wildlife; raccoons (51 cases; 68.9%) were the primary host confirmed with RABV infection, followed by skunk and fox (8 cases each; 10.8%), bobcat (5 cases; 6.8%), and bats (2 cases; 2.7%). Only 5 domestic animals (6.3% of the positive cases) tested positive during our study period; one from each of the bovine, canine, caprine, equine, and feline species. Hence, the sylvatic cycle plays the predominant role in circulating RABV infection in our study area.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11185117/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140922404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}