Journal of Veterinary Diagnostic Investigation最新文献_第9页

Streptococcus hillyeri isolated from septic pleuritis in a horse. 从马脓毒性胸膜炎中分离出希利氏链球菌。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-01-07 DOI: 10.1177/10406387241306724

Ervin Albert, Imre Biksi, Levente Laczkó, László Miló, Karolina Cseri, Renáta Bőkényné Tóth, Dalma Papp, Dóra Halmay, Emese Bódai, Zoltán Bakos

{"title":"Streptococcus hillyeri isolated from septic pleuritis in a horse.","authors":"Ervin Albert, Imre Biksi, Levente Laczkó, László Miló, Karolina Cseri, Renáta Bőkényné Tóth, Dalma Papp, Dóra Halmay, Emese Bódai, Zoltán Bakos","doi":"10.1177/10406387241306724","DOIUrl":"10.1177/10406387241306724","url":null,"abstract":"Here we report the isolation of Streptococcus hillyeri from a thoracic sample from a horse. A 17-y-old Hungarian Sport Horse mare was referred to the equine clinic of the University of Veterinary Medicine Budapest, Hungary, with suspected pleuritis. Upon arrival, the horse was febrile and had tachycardia, severe inspiratory dyspnea, and tachypnea. Thoracic ultrasonography revealed severe bilateral pleural effusion, and a large area of lung consolidation. After sampling of both hemithoraces, 66 L of turbid exudate were drained. Based on these findings, a tentative diagnosis of septic pleuritis was made, and the horse was immediately started on a course of broad-spectrum antibiotics, a NSAID, an anticoagulant, and intravenous fluids. Despite intensive care, the clinical parameters deteriorated, and the horse was euthanized 6 d later. Cytology confirmed septic pleuritis, with short chains or groups of coccoid bacteria. Anaerobic culture yielded gram-positive cocci from both hemithoraces in almost pure culture, which we identified as S. hillyeri by 16S rDNA and whole-genome analysis. Additionally, we identified 4 previously unassigned Streptococcus sp. sequences as S. hillyeri. Of these, 3 were obtained from aborted equine fetuses and a fourth from a donkey mastitis case, supporting the pathogenic nature of S. hillyeri in these host species.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"393-397"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11707762/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of a semiquantitative scoring system for mild-to-moderate gill lesions in Atlantic salmon reared in recirculating aquaculture systems in Norway. 挪威循循环水产养殖系统中大西洋鲑鱼轻度至中度鳃损伤的半定量评分系统评估。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-01-28 DOI: 10.1177/10406387241310900

Thomas Amlie, Alf Dalum, Marit Stormoen, Øystein Evensen

{"title":"Assessment of a semiquantitative scoring system for mild-to-moderate gill lesions in Atlantic salmon reared in recirculating aquaculture systems in Norway.","authors":"Thomas Amlie, Alf Dalum, Marit Stormoen, Øystein Evensen","doi":"10.1177/10406387241310900","DOIUrl":"10.1177/10406387241310900","url":null,"abstract":"Compromised gill health is a critical cause of forfeited welfare in Atlantic salmon farming. Detecting and quantifying the early onset of gill disease is important to reveal initial inflicting stimuli. We collected gill samples of 45 Atlantic salmon from 2 commercial recirculating aquaculture systems (RASs) spanning fry-to-market-size fish with no clinical signs of gill disease. Gill samples were assessed histologically by 3 independent raters with different levels of experience. Semiquantitative scoring for 7 types of gill changes was carried out for 10 filaments per gill (450 filaments total) over 3 rounds on anonymized samples. Scores were summarized for each type of gill change. The assumed clinical relevance for each change was transformed into a category score, followed by an assessment of agreement within (intra) and between (inter) raters. A generalized linear model estimated the difference in score levels between raters. For each rater, intra-rater agreement was high for 6 gill changes and moderate for 1 gill change. Inter-rater agreement was moderate to almost-perfect, except for 2 gill changes; generalized linear model regression revealed systematic differences in score usage between the raters. Our scoring protocol worked satisfactorily for mucous cell amount, lamellar clubbing, lamellar hypertrophy and/or hyperplasia, and aneurysms, despite different levels of expertise in histologic evaluation. Intra-rater agreement was consistent, but differences existed for interlamellar hypercellularity, lamellar inflammation, and degeneration. Scoring subclinical gill changes is a challenge, and our scoring system for mild-to-moderate lesions may enable early intervention to limit the detrimental effects of poor gill health in RAS farming.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"252-262"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11775946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peliosis hepatis and hepatic fibrosis in a dog infected with multiple Bartonella species. 犬感染多种巴尔通体并发肝纤维化。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-01-07 DOI: 10.1177/10406387241310868

Cynthia Robveille, Cody Atkinson, Jerry Cowart, Ricardo G Maggi, Neelesh Narurkar, Edward B Breitschwerdt

{"title":"Peliosis hepatis and hepatic fibrosis in a dog infected with multiple Bartonella species.","authors":"Cynthia Robveille, Cody Atkinson, Jerry Cowart, Ricardo G Maggi, Neelesh Narurkar, Edward B Breitschwerdt","doi":"10.1177/10406387241310868","DOIUrl":"10.1177/10406387241310868","url":null,"abstract":"A 13-y-old, spayed female dog had regenerative anemia, lymphopenia, hypoalbuminemia, and elevated hepatic biochemical parameters. Liver biopsy revealed hepatic peliosis (hepatic sinusoidal angiectasis), frequently associated with perisinusoidal fibrosis. The dog was seroreactive to Bartonella antigens by indirect fluorescent antibody assays, and quantitative PCR from blood identified Bartonella vinsonii subsp. berkhoffii genotype II. The dog was euthanized 9 mo later because of acute decompensation. Autopsy revealed icteric adipose tissues, end-stage liver, and abdominal effusion. Microscopically, there was marked mixed-cell chronic hepatitis with hepatocellular loss, nodular hepatocellular regeneration, and capillary proliferation. Retrospective molecular testing documented B. koehlerae and B. rochalimae DNA in the dog's blood at 2 or more times during liver disease progression. B. koehlerae DNA was also amplified and sequenced from the autopsy sample of liver. Our case emphasizes that Bartonella infection may be associated with hepatic peliosis and end-stage liver in dogs and expands the spectrum of Bartonella species that potentially play a role in canine hepatic diseases.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"358-362"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11707757/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Frequency and antimicrobial susceptibility of Staphylococcus aureus isolated from clinical bovine mastitis cases in British Columbia, Canada. 加拿大不列颠哥伦比亚省临床牛乳腺炎病例分离的金黄色葡萄球菌的频率和抗菌药物敏感性。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2024-12-26 DOI: 10.1177/10406387241306096

Melissa Leon, Joseph Rubin, Stephen Raverty, Kazal Ghosh

{"title":"Frequency and antimicrobial susceptibility of Staphylococcus aureus isolated from clinical bovine mastitis cases in British Columbia, Canada.","authors":"Melissa Leon, Joseph Rubin, Stephen Raverty, Kazal Ghosh","doi":"10.1177/10406387241306096","DOIUrl":"10.1177/10406387241306096","url":null,"abstract":"Staphylococcus aureus is one of the most important bacteria responsible for clinical bovine mastitis globally, leading to significant economic losses in the dairy industry. Antimicrobials used to treat and prevent mastitis can lead to antimicrobial resistance (AMR) in S. aureus. We retrospectively evaluated AMR of S. aureus isolates from clinical bovine mastitis cases submitted to the Animal Health Centre in British Columbia from 2013 to May 2024. S. aureus was isolated from 15.0% of submitted bovine milk samples. Antimicrobial susceptibility testing was done on 611 of 1,347 S. aureus isolates. No methicillin-resistant S. aureus isolates were detected based on cefoxitin susceptibility test results, which is encouraging. The highest frequencies of resistance were found for penicillin (46.6%) and ampicillin (42.1%). The lowest frequencies of resistance were to ceftiofur and sulfamethoxazole-trimethoprim (0.2% each) and cephalothin (0.3%). AMR trends over our study period were generally stable, except for penicillin and ampicillin; penicillin resistance increased from 15.2% to 71.1%, and ampicillin resistance increased from 18.2% to 70.1%. The information in our study could help guide clinicians when choosing antimicrobial treatments to treat mastitis caused by S. aureus, particularly in the province of British Columbia. Because S. aureus has a broad host range and is of importance to both human and veterinary medicine, continued monitoring to detect the emergence of resistance is warranted.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"371-374"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11672364/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mixed histiocytic sarcoma in a Bernese Mountain Dog. 伯尔尼山犬混合性组织细胞肉瘤。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-01-27 DOI: 10.1177/10406387241312308

Jillian Hickey, R Adam Harris, Stacy D Meola, Samuel Jennings, Peter F Moore, William Vernau, Kayla Harding, Douglas H Thamm, Lisa J Schlein

{"title":"Mixed histiocytic sarcoma in a Bernese Mountain Dog.","authors":"Jillian Hickey, R Adam Harris, Stacy D Meola, Samuel Jennings, Peter F Moore, William Vernau, Kayla Harding, Douglas H Thamm, Lisa J Schlein","doi":"10.1177/10406387241312308","DOIUrl":"10.1177/10406387241312308","url":null,"abstract":"An 8-y-old, spayed female Bernese Mountain Dog was presented to a referral center for evaluation of right thoracic limb lameness and previously suspected Evans syndrome that had been poorly responsive to immunosuppressive therapy. Based on review of examination findings and laboratory data, Evans syndrome was deemed unlikely and hemophagocytic histiocytic sarcoma (HHS) was strongly suspected. On blood smear evaluation, atypical, histiocytic cells were noted, some of which exhibited siderophagia. Considering that circulating cells are not typically observed in dogs with HHS, additional diagnostic investigation was performed. Autopsy and histopathology revealed that the dog had a mixed form of HS (dendritic-cell origin HS in the lung, and HHS in the spleen, liver, and bone marrow), and immunocytochemical characterization of cultured cells derived from blood suggested that the cells were of dendritic HS origin, rather than HHS origin, as originally suspected. Whole-exome sequencing revealed genetic similarity between cell lines derived from lung tissue and blood, providing additional evidence of the relatedness of these 2 cell populations. Our case highlights the rare entity of mixed HS and typifies the inherent challenges in classifying rare, atypical, circulating neoplastic cells.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"317-323"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773501/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of bovine respiratory disease complex-related pathogens in nasopharynx-associated lymphoid tissue. 牛呼吸道疾病复合体相关病原体在鼻咽相关淋巴组织中的检测。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-02-17 DOI: 10.1177/10406387251318415

Asmaa A Hegazy, Mutsumi Nakai, Naoyuki Fuke, Amaal E Hussein, Hiroaki Kondo, Takuya Hirai

{"title":"Detection of bovine respiratory disease complex-related pathogens in nasopharynx-associated lymphoid tissue.","authors":"Asmaa A Hegazy, Mutsumi Nakai, Naoyuki Fuke, Amaal E Hussein, Hiroaki Kondo, Takuya Hirai","doi":"10.1177/10406387251318415","DOIUrl":"10.1177/10406387251318415","url":null,"abstract":"Bovine respiratory disease complex (BRDC) is a multi-etiologic disease that significantly impacts the cattle industry. Nasopharynx-associated lymphoid tissue (NALT) is the nasal mucosal immune system that protects against various pathogens; however, reports on its pathology are limited. We compared the pathologic impact of BRDC-related viruses on the NALT and lungs of 10 pneumonic and 3 negative control Japanese black (JB) calves. Three bovine viral diarrhea virus (BVDV) persistently infected Holstein calves were examined as positive control; all 3 calves had BVDV in situ hybridization (ISH)-positive signals in NALT follicular macrophages and lymphocytes, suggesting viral replication in these cells. NALT and lungs of pneumonic JB calves had weak-positive BVDV results in PCR and negative results in ISH, indicating late-stage transient BVDV infection. The finding of BVDV in unvaccinated pneumonic JB calves confirmed the involvement of a BVDV field strain. PCR detection of bovine coronavirus (BCoV) and bovine respiratory syncytial virus (BRSV) in NALT along with ISH BCoV-positive signals in NALT epithelial cells confirms infection by those viruses. Pneumonic JB calves had nasopharyngitis and pneumonia, with the same bacteria present in nasopharyngeal swabs and lungs, indicating that, in these animals, the bacteria proliferating in the nasopharynx probably migrated to the lungs via inhalation. BVDV transient infection in the NALT may induce local immunosuppression; BCoV and BRSV infections are thought to damage epithelial cells, facilitating bacterial infection of the NALT and lungs. Our results confirm that bovine NALT is a location for viral replication and may be associated with BRDC development in cattle.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"284-297"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11833804/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143441086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ovarian neuroglial choristoma in a dog. 犬卵巢神经胶质瘤。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-02-02 DOI: 10.1177/10406387251316831

Na-Young Lee, Jeong-Seop Oh, Dae-Yong Kim, Tae-Kyun Im, Dae Young Kim

引用次数: 0

Aspergillosis in 41 wild bird species in the eastern United States: a 22-year retrospective review. 美国东部 41 种野生鸟类的曲霉菌病：22 年的回顾性研究。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-01-27 DOI: 10.1177/10406387241313484

C Robert Stilz, Melanie R Kunkel, M Kevin Keel, Heather Fenton, Alisia A W Weyna, Kevin D Niedringhaus, Victoria A Andreasen, Amy S McKinney, Grazieli Maboni, Nicole M Nemeth

{"title":"Aspergillosis in 41 wild bird species in the eastern United States: a 22-year retrospective review.","authors":"C Robert Stilz, Melanie R Kunkel, M Kevin Keel, Heather Fenton, Alisia A W Weyna, Kevin D Niedringhaus, Victoria A Andreasen, Amy S McKinney, Grazieli Maboni, Nicole M Nemeth","doi":"10.1177/10406387241313484","DOIUrl":"10.1177/10406387241313484","url":null,"abstract":"Aspergillosis is the most commonly and widely reported fungal infection in birds. Disease development is often secondary to stressors that cause immunocompromise, and it is typically regarded as a disease of captivity. We retrospectively evaluated data from 133 birds diagnosed with aspergillosis at the Southeastern Cooperative Wildlife Disease Study from 2001-2023 to assess diversity and relative frequency across avian taxa, gross and histologic lesion patterns, and comorbidities. Of 10 taxonomic orders represented, Charadriiformes (shorebirds; n = 35) and Accipitriformes (raptors; n = 32) were most common. Among them, the laughing gull (Leucophaeus atricilla; n = 20) and bald eagle (Haliaeetus leucocephalus; n = 14) were infected most commonly. Gross lesions were most frequent in lung (n = 80), air sac (n = 71), or celomic cavity lining (n = 42). Four distinct gross lesion patterns were identified: 1) tan caseous plaques (n = 106), 2) hollow masses lined with mold (n = 26), 3) red pulmonary nodules (n = 15), and 4) necrotic brown plaques (n = 3). Histologically, fungal hyphae were most common in lung (n = 107) and air sac (n = 49). Comorbidities were diagnosed in 67 birds with a spectrum of viral (n = 19), bacterial (n = 11), parasitic (n = 6), other fungal (n = 4), and non-infectious (n = 50) causes. Six birds each were diagnosed with highly pathogenic avian influenza or salmonellosis. Twenty-two birds were emaciated. Free-ranging birds are susceptible to myriad stressors that can predispose them to the development of aspergillosis.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"305-316"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic characterization of diagnostic epitopes of cardiac troponin I in African rhinoceros. 非洲犀牛心肌肌钙蛋白I诊断表位的遗传特征。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2024-12-26 DOI: 10.1177/10406387241305323

Yolandi Rautenbach, Sven D C Parsons, Angelika K Loots, Amelia Goddard, Leith C R Meyer, Peter E Buss, Emma H Hooijberg

{"title":"Genetic characterization of diagnostic epitopes of cardiac troponin I in African rhinoceros.","authors":"Yolandi Rautenbach, Sven D C Parsons, Angelika K Loots, Amelia Goddard, Leith C R Meyer, Peter E Buss, Emma H Hooijberg","doi":"10.1177/10406387241305323","DOIUrl":"10.1177/10406387241305323","url":null,"abstract":"African rhinoceros undergo chemical immobilization and prolonged transport during translocations for conservation purposes and, hence, experience several pathophysiologic changes, including skeletal muscle injury. Potential concurrent myocardial injury has not been investigated due to a lack of validated immunoassays. We aimed to use inferred cardiac troponin I (cTnI) amino acid sequences of southern white (Ceratotherium simum simum) and southern-central black (Diceros bicornis minor) rhinoceros to assess the potential usefulness of several commercial cTnI immunoassays for detecting cTnI in African rhinoceros. We extracted RNA from the myocardium of deceased rhinoceros (2 white, 1 black rhinoceros) followed by primer design, cDNA synthesis via RT-PCR, and Sanger sequencing. The inferred cTnI amino acid sequences were obtained from the mRNA transcript sequences. The homology of epitope binding sites recognized by capture and detection antibodies in 6 human immunoassays was visually evaluated using aligned inferred rhinoceros cTnI amino acid sequences. Percentage identity between white and black rhinoceros cDNA nucleotide sequences was 99%; inferred amino acid sequences were identical. There were 5 amino acid differences between humans and rhinoceros in the epitope binding sites of immunoassay antibodies; 5 assays contained antibodies against epitopes that were not conserved. For one assay, the single capture antibody targeted a short heterologous epitope (residue 87-91), and cross-reactivity with rhinoceros cTnI was deemed unlikely. For the other 5 assays, complete antibody-epitope homology, or the inclusion of multiple detection or capture antibodies, or targeting of long epitopes, indicated that these assays could be suitable for further investigation of cTnI measurement in African rhinoceros.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"263-271"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11672365/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of the frequency and selected prevalence factors of equid alphaherpesvirus 4 viremia in horses with acute onset of fever and respiratory signs. 有急性发热和呼吸体征的马患马甲疱疹病毒4型病毒血症的频率和选定流行因素的调查。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-01-28 DOI: 10.1177/10406387241308080

Nicola Pusterla, Samantha Barnum, Kaila Lawton, Bryant Craig, Kaitlyn James

{"title":"Investigation of the frequency and selected prevalence factors of equid alphaherpesvirus 4 viremia in horses with acute onset of fever and respiratory signs.","authors":"Nicola Pusterla, Samantha Barnum, Kaila Lawton, Bryant Craig, Kaitlyn James","doi":"10.1177/10406387241308080","DOIUrl":"10.1177/10406387241308080","url":null,"abstract":"Equid alphaherpesvirus 4 (EqAHV4; Orthoherpesviridae, Varicellovirus equidalpha4; equine rhinopneumonitis virus) has seldom been associated with complications such as abortion and myeloencephalopathy, given the low tendency of this virus to induce viremia. We investigated the frequency of EqAHV4 viremia in horses with fever and respiratory signs. Case selection included all equids with EqAHV4 quantitative real-time PCR (qPCR)-positive nasal secretions (defined as EqAHV4 qPCR-positive cases) submitted to a diagnostic laboratory. Controls consisted of each case submitted before and after each EqAHV4 qPCR-positive case. Purified nucleic acid from blood samples collected from EqAHV4 qPCR-positive horses and control cases was tested for EqAHV4 by qPCR. We selected 183 EqAHV4 qPCR-positive horses and 376 EqAHV4 qPCR-negative horses. In general, EqAHV4 qPCR-positive horses were younger, and had a lower rate of anorexia and a higher rate of nasal discharge compared to the EqAHV4 qPCR-negative horses. A total of 25 of 183 (13.7%) horses with EqAHV4 qPCR-positive nasal secretions tested qPCR-positive for EqAHV4 in blood. EqAHV4 viremic horses were significantly younger (p < 0.015 for group <1-y-old) and had a significantly higher occurrence of distal limb edema (p < 0.05) than EqAHV4 non-viremic horses. Our data support the observation that EqAHV4 viremia is rarely detected in EqAHV4-infected horses, which explains the low level of reported complications, such as abortion and myeloencephalopathy.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"349-353"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11775911/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0