A fast molecular tool for sexing Gibber Italicus, song thrush, and redwing birds.

IF 1.2 3区 农林科学 Q3 VETERINARY SCIENCES
Emanuele D'Anza, Sara Albarella, Ilaria Cascone, Mariagiulia Pugliano, Francesca Ciotola, Vincenzo Peretti
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引用次数: 0

Abstract

Gibber Italicus, song thrush (Turdus philomelus), and redwing (Turdus iliacus) are 3 bird species in which sexual dimorphism is not readily apparent. Therefore, molecular sexing is a valuable tool for breeding, selection, and conservation purposes. We compared DNA extraction by commercial kit and an alkaline method from feathers, then developed a molecular method for sexing these species using the P2/P8 and CHD1F/CHD1R primer pairs. Both protocols were successful in extracting DNA; the alkaline method is more cost-effective, whereas the commercial kit protocol is easier to standardize. All DNA samples were successfully amplified using both the P2/P8 and CHD1F/1R primer pairs. The use of the CHD1F/1R primer pair is notably advantageous as it produces fragments of different lengths of the sex chromosomes, making them easily distinguishable via electrophoresis. The sequence analysis of the amplicons obtained with the CHD1F/1R primer pair revealed specific single-nucleotide polymorphisms for song thrush and redwing, which could be used as markers to differentiate between the 2 species if required.

一个快速的分子工具,对意大利吉布,画眉和红翼鸟进行性别鉴定。
意大利长臂猿、鸣鸫(Turdus philomelus)和红翅鸫(Turdus iliacus)是三种性别二态性不明显的鸟类。因此,分子性别是一种有价值的工具,用于育种,选择和保护目的。我们比较了商业试剂盒和碱法提取羽毛的DNA,然后利用P2/P8和CHD1F/CHD1R引物对建立了一种分子方法来鉴定这些物种的性别。两种方法都成功地提取了DNA;碱性方法更具成本效益,而商业试剂盒协议更容易标准化。使用P2/P8和CHD1F/1R引物对成功扩增所有DNA样本。使用CHD1F/1R引物对是非常有利的,因为它产生不同长度的性染色体片段,使它们很容易通过电泳区分。用CHD1F/1R引物对扩增子进行序列分析,发现了鸫和红翅的特异性单核苷酸多态性,可以作为区分两种鸟类的标记。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
3.00
自引率
6.70%
发文量
127
审稿时长
6-16 weeks
期刊介绍: The Journal of Veterinary Diagnostic Investigation (J Vet Diagn Invest) is an international peer-reviewed journal published bimonthly in English by the American Association of Veterinary Laboratory Diagnosticians (AAVLD). JVDI is devoted to all aspects of veterinary laboratory diagnostic science including the major disciplines of anatomic pathology, bacteriology/mycology, clinical pathology, epidemiology, immunology, laboratory information management, molecular biology, parasitology, public health, toxicology, and virology.
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