在模拟装运环境条件下评估毛滴虫胎儿在各种培养基中的稳定性，用于RT-qPCR检测。

IF 1.2 3区农林科学 Q3 VETERINARY SCIENCES

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-04-16 DOI:10.1177/10406387251330297

SaraBeth Boggan, Ryan B Williams, Jennifer H Koziol

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引用次数: 0

摘要

胎儿毛滴虫是牛中一种重要的生殖病原体，样品采集、处理、运输和检测过程对监测行动构成了相当大的挑战。进展包括利用反转录实时定量PCR直接检测胎儿弓形虫。在0°C、20°C、37°C和54.5°C的储存温度下，我们比较了样品在3种不同介质（PBS、无菌生理盐水和乳酸林格溶液）中24-120 h的稳定性，以模拟样品在不同环境温度下的运输。

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Evaluating the stability of Tritrichomonas foetus in various media under simulated environmental conditions of shipment for RT-qPCR assays.

Tritrichomonas foetus is a notable reproductive pathogen in cattle, and the processes of sample collection, handling, transportation, and testing present considerable challenges for surveillance initiatives. Advancements include the development of a direct detection method for T. foetus using reverse-transcription quantitative real-time PCR. We compared the stability of samples in 3 different media: PBS, sterile saline, and lactated Ringer solution over periods of 24-120 h, with storage temperatures of 0°C, 20°C, 37°C, and 54.5°C to mimic shipping of samples in various environmental temperatures.

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来源期刊

Journal of Veterinary Diagnostic Investigation 农林科学-兽医学

CiteScore

3.00

自引率

6.70%

发文量

127

审稿时长

6-16 weeks

期刊介绍： The Journal of Veterinary Diagnostic Investigation (J Vet Diagn Invest) is an international peer-reviewed journal published bimonthly in English by the American Association of Veterinary Laboratory Diagnosticians (AAVLD). JVDI is devoted to all aspects of veterinary laboratory diagnostic science including the major disciplines of anatomic pathology, bacteriology/mycology, clinical pathology, epidemiology, immunology, laboratory information management, molecular biology, parasitology, public health, toxicology, and virology.