Emergence of hemorrhagic septicemia caused by MLST type ST64 Pasteurella multocida in a European fallow deer population in Hungary.

IF 1.2 3区 农林科学 Q3 VETERINARY SCIENCES
Károly Erdélyi, Krisztina Pintér, Boglárka Pollák, Erika Bakcsa, Levente Szeredi, Pál Lehotzky, Tibor Magyar
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引用次数: 0

Abstract

A sudden mass mortality involving 44 fallow deer (Dama dama) occurred in September 2020 at a game management area near Budapest, Hungary. The die-off affected both sexes and all age classes equally. On postmortem examination of 2 subadults and 1 fawn, we found average body condition, diffuse subcutaneous edema, swelling of peripheral lymph nodes, marked hemorrhages in lymph nodes along the esophagus and trachea, and left ventricular dilation. We isolated Pasteurella-like colonies from spleen, liver, small intestine, lung, and brain samples of 7 animals, and identified them as P. multocida serotype B:2 by amplifying kmt1, toxA, and hyaC-hyaD genes, capsular typing by PCR, and serotyping by agar gel diffusion precipitation test, which established the diagnosis of hemorrhagic septicemia as the cause of the outbreak. By further analysis, we determined that all of our P. multocida isolates encoded ptfA, fimA, hsf-2, nanH, hgbA, and pfhA, but we did not detect genes encoding toxA, hsf-1, tbpA, or tadD. We identified our strains by RIRDC MLST as sequence type (ST)122, characteristic for strains causing hemorrhagic septicemia, while using multi-host MLST analysis we assigned these strains to ST64 clustering with P. multocida strains from the 2015 hemorrhagic septicemia outbreak of saiga antelopes in Kazakhstan. We concluded that increased population density and aggregation due to limited drinking water availability played a role in initiating the outbreak.

由ST64型多杀性巴氏杆菌引起的出血性败血症在匈牙利的欧洲鹿群中出现。
2020年9月,在匈牙利布达佩斯附近的一个狩猎管理区发生了44头黇鹿(Dama Dama)的突然大规模死亡。这种死亡对男女和各年龄段的人都有影响。在2例亚成虫和1例小鹿的尸检中,我们发现身体状况一般,弥漫性皮下水肿,周围淋巴结肿胀,食管和气管淋巴结明显出血,左心室扩张。我们从7只动物的脾脏、肝脏、小肠、肺和脑样本中分离到巴氏杆菌样菌落,通过kmt1、toxA和hyaC-hyaD基因扩增、PCR荚膜分型和琼脂凝胶扩散沉淀法分型,鉴定为多杀性巴氏杆菌血清型B:2,从而确定出血性败血症是此次暴发的病因。通过进一步分析,我们确定所有的多杀p.a分离株都编码ptfA、fimA、hsf-2、nanH、hgbA和pfhA,但我们没有检测到编码toxA、hsf-1、tbpA或tadD的基因。我们通过RIRDC MLST鉴定菌株为序列型(ST)122,这是引起出血性败血症的菌株的特征,而通过多宿主MLST分析,我们将这些菌株与2015年哈萨克斯坦赛加羚羊出血性败血症暴发的多杀p.a ltocida菌株归为ST64聚类。我们的结论是,由于饮用水供应有限,人口密度和聚集性增加在引发疫情方面发挥了作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
3.00
自引率
6.70%
发文量
127
审稿时长
6-16 weeks
期刊介绍: The Journal of Veterinary Diagnostic Investigation (J Vet Diagn Invest) is an international peer-reviewed journal published bimonthly in English by the American Association of Veterinary Laboratory Diagnosticians (AAVLD). JVDI is devoted to all aspects of veterinary laboratory diagnostic science including the major disciplines of anatomic pathology, bacteriology/mycology, clinical pathology, epidemiology, immunology, laboratory information management, molecular biology, parasitology, public health, toxicology, and virology.
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