Journal of Veterinary Science最新文献

{"title":"PCR-based detection of hereditary mutations in <i>SLC2A9</i>, <i>BTBD17</i>, and <i>NECAP1</i> among native Korean dog breeds.","authors":"Mingue Kang, Byeongyong Ahn, Jaeyeol Shin, Chankyu Park","doi":"10.4142/jvs.25047","DOIUrl":"10.4142/jvs.25047","url":null,"abstract":"<p><strong>Importance: </strong>Understanding the susceptibility of native Korean dogs to genetic diseases is important for animal welfare and breeding. However, little is known regarding the segregation of genetic variations associated with genetic diseases among native Korean dog breeds, highlighting the need for further research in this area.</p><p><strong>Objective: </strong>A genome-wide analysis was conducted using the available whole genome sequencing (WGS) results of DongGyeongi, Sapsaree, and Jindo dogs to evaluate the presence of disease-associated mutations and develop polymerase chain reaction (PCR)-based typing methods.</p><p><strong>Methods: </strong>Using WGS data from 32 individuals of DongGyeongi, Sapsaree, and Jindo dogs, variant calling was used to identify variants associated with canine genetic diseases in Online Mendelian Inheritance in Animals. Additionally, allele-specific PCR-based typing methods for the identified mutations were developed.</p><p><strong>Results: </strong>Four different variants were identified: NC_006583.3:g.54192143C>G in the <i>T</i> gene associated with the bobtail phenotype, NC_006585.3:g.69456869G>T in <i>SLC2A9</i> associated with urolithiasis, NC_006591.3:g.6048201_6048202insG in <i>BTBD17</i> associated with abortion, and NC_006607.3:g.48121642G>A in <i>MLPH</i> associated with coat color dilution. The <i>BTBD17</i> mutant allele was present in all breeds (frequency = 0.33), whereas the <i>MLPH</i> mutant allele was found only in DongGyeongi (0.18) and Sapsaree breeds (0.20). The <i>T</i> and <i>SLC2A9</i> mutant alleles were specific to DongGyeongi (0.20 and 0.18, respectively).</p><p><strong>Conclusions and relevance: </strong>Genome-wide analysis was conducted to identify the causative mutations of genetic diseases in native Korean dogs. The observed frequencies of the identified variants differed among native Korean dog breeds. The typing methods used to detect variant alleles in this study will contribute to improving the genetic robustness of native Korean dog breeds.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 4","pages":"e48"},"PeriodicalIF":1.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12329054/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blood pressure agreement between ideal and loose-fitting cuffs in anesthetized dogs.","authors":"Jennifer Pelchat, Anthony Carr, Shannon G Beazley","doi":"10.4142/jvs.25081","DOIUrl":"10.4142/jvs.25081","url":null,"abstract":"<p><strong>Importance: </strong>Indirect blood pressure monitoring is used frequently in veterinary medicine. Blood pressure cuff looseness has not been investigated as a cause of erroneous measurements.</p><p><strong>Objective: </strong>To determine if cuff looseness affects blood pressure measurements in healthy anesthetized dogs.</p><p><strong>Methods: </strong>Between December 2020 and May 2022 at an institutional practice, 62 anesthetized healthy dogs were separated into two groups: ≤ 20 kg and > 20 kg. Tail base circumference of each dog was measured, and baseline was defined as ideal (0% looseness factor). The cuff was manually loosened sequentially from 0% to 2%, 5%, 8%, 10% and 15% looseness factors. High definition oscillometry was used to measure systolic (SAP), mean (MAP) and diastolic (DAP) arterial blood pressures. Bland and Altman for repeated measures was used to analyze SAP, MAP and DAP measurements of baseline and each looseness factor. Acceptable bias and limits of agreement (LoA) were set using American College of Veterinary Internal Medicine guidelines.</p><p><strong>Results: </strong>All biases were acceptable. In dogs ≤ 20 kg, LoA for all SAP looseness factors and MAP looseness factors of 10% and 15% did not fall within the guidelines. In dogs > 20 kg, LoA for all measurements except SAP 5%, 8% and 10% looseness factors fell within the guidelines.</p><p><strong>Conclusions and relevance: </strong>Loosening a cuff up to 15% did not result in significant changes to blood pressure measurements of healthy anesthetized dogs using high definition oscillometry.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 4","pages":"e51"},"PeriodicalIF":1.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12329061/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of pharmacokinetic and pharmacodynamic properties of allopurinol and benzbromarone in a quail model of hyperuricemia.","authors":"ShaoJun Zheng, YaQin Bu, Sheng Li, NaiDong Chen","doi":"10.4142/jvs.24313","DOIUrl":"10.4142/jvs.24313","url":null,"abstract":"<p><strong>Importance: </strong>Hyperuricemia (HUA) is a major metabolic disorder in poultry, leading to gout and kidney damage, which affects farm productivity. Accordingly, understanding the pharmacodynamics (PD) and pharmacokinetics (PK) of uric acid-lowering drugs is essential for improving the treatment strategies in poultry.</p><p><strong>Objective: </strong>This study examined the PD and PK characteristics of allopurinol and benzbromarone, two uric acid-lowering drugs, in a quail model of HUA.</p><p><strong>Methods: </strong>A hyperuricemic quail model was established using a high-purine diet. Allopurinol and benzbromarone were administered orally. Blood samples were taken and analyzed for the drug concentrations using high-performance liquid chromatography. Pathological examinations were conducted to assess kidney damage.</p><p><strong>Results: </strong>Both drugs lowered the serum uric acid levels. On the other hand, the allopurinol treatment exhibited lower urea and creatinine levels than benzbromarone, indicating potential advantages in reducing kidney damage. Consistent with these findings, the pathological examinations revealed more pronounced kidney damage in the benzbromarone-treated group than in the allopurinol group. PK analysis showed that allopurinol exhibited faster absorption and elimination kinetics than benzbromarone. Both drugs showed a wide distribution in various tissues, with allopurinol and its active metabolite displaying higher excretion levels.</p><p><strong>Conclusions and relevance: </strong>This paper reports the absorption, distribution, metabolism, and excretion processes of allopurinol and benzbromarone in a quail model of HUA. These findings help better understand the PK characteristics of these drugs and promote the use of high uric acid therapy in poultry. In addition, this quail model is a valuable tool for future research on HUA and drug interventions.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 4","pages":"e34"},"PeriodicalIF":1.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12329059/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection and genetic evolution analysis of porcine reproductive and respiratory syndrome virus in Chengdu from January 2021 to December 2022.","authors":"Hongyu Zhong, Kerong Li, Min Li, Kun Zhang, Yi Zhang, Luqi Liang, Haifeng Liu, Zhijun Zhong, Guangneng Peng, Xiaoxiao Zhou, Ziyao Zhou","doi":"10.4142/jvs.24277","DOIUrl":"10.4142/jvs.24277","url":null,"abstract":"<p><strong>Importance: </strong>Pandemics involving the porcine reproductive and respiratory syndrome virus (PRRSV) impact swine health and productivity, causing economic devastation, especially in large-scale feeding areas.</p><p><strong>Objective: </strong>This study examined the prevalence and genetic variations of PRRSV in Chengdu, China, a vital transportation hub and the main area of pig breeding in southwest China, between January 2021 and December 2022.</p><p><strong>Methods: </strong>The samples were collected from 13 key pig-feeding areas in Chengdu. The positive PRRSV samples were detected using a real-time quantitative polymerase chain reaction. The genetic variations of the PRRSV were analyzed by amplifying the open reading frame 5 (ORF5) gene and comparing it with relevant reference sequences.</p><p><strong>Results: </strong>The PRRSV positive rate in Chengdu was 11.42% (276/2,416), including sublines 8.7, 5.1, 3.0, and 1.8. The ORF5 gene sequences in the samples had no insertion or deletion of amino acids but widespread variations, including the 13th and 151st positions, which were associated with viral virulence.</p><p><strong>Conclusions and relevance: </strong>The PRRSV pandemic in Chengdu was relevant, and recombination between vaccine strains and field strains can contribute to vaccine immune failure.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":" ","pages":"e36"},"PeriodicalIF":1.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12329057/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical utility of two-dimensional shear wave elastography in two dogs with presumptive toxic hepatopathy.","authors":"Junyoung Kim, Dai Jung Chung, Soyoung Lee, Jihye Choi, Junghee Yoon","doi":"10.4142/jvs.25083","DOIUrl":"10.4142/jvs.25083","url":null,"abstract":"<p><strong>Importance: </strong>Shear wave elastography (SWE) is a non-invasive ultrasound technique for assessing tissue stiffness. This study examined the clinical utility of two-dimensional SWE (2D-SWE) in two dogs diagnosed with presumptive toxic hepatopathy.</p><p><strong>Case presentation: </strong>The first case involved a 9-year-old Pompitz that presented with anorexia, vomiting, and diarrhea following garlic and onion ingestion. Laboratory tests revealed anemia, elevated liver enzyme levels, and diabetic ketoacidosis. Ultrasonography demonstrated increased hepatic echogenicity and elevated SWE values. Despite intensive treatment, the dog's condition deteriorated, with progressive increases in liver enzyme levels and SWE values, resulting in euthanasia. The second case involved a 1-year-old Maltipoo that presented vomiting, depression, and abdominal pain, with no reported history. Laboratory findings indicated elevated liver enzyme and bile acid levels. Ultrasonography revealed increased hepatic echogenicity and SWE values. Following treatment for acute toxic hepatopathy, the dog exhibited significant improvement within 3 days, with normalization of hepatic echogenicity, SWE values, and liver enzyme levels.</p><p><strong>Conclusions and relevance: </strong>These cases demonstrate the clinical relevance of 2D-SWE in assessing hepatic damage, tracking therapeutic response, and informing prognosis in presumptive toxic hepatopathy.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 4","pages":"e45"},"PeriodicalIF":1.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12329062/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial Cluster Analysis and Key Influencing Factors of African Swine Fever in China (2018-2021).","authors":"Liyuan Wang, Latiffah Binti Hassan, Ooi Peck Toung, Xia Li","doi":"10.4142/jvs.24307","DOIUrl":"10.4142/jvs.24307","url":null,"abstract":"<p><strong>Importance: </strong>African swine fever (ASF) is a significant transboundary disease with profound global economic impacts. ASF, caused by the <i>Asfarviridae</i> virus, is highly resilient and has caused substantial economic losses in affected regions, with no available vaccination or treatment. We conducted a comprehensive analysis of the spatial clusters and potential factors contributing to the ASF epidemic in China from August 2018 to April 2021.</p><p><strong>Objective: </strong>The study aimed to trace ASF transmission, map the spatial distribution and spread dynamics, and model the spread to evaluate the potential control strategies.</p><p><strong>Methods: </strong>Standardized deviational ellipse analysis was used to identify the directionality and distribution of ASF outbreaks. Ordinary least squares regression and geographically weighted regression were applied to account for spatial heterogeneity and explore the localized relationships within the data set.</p><p><strong>Results: </strong>The ASF transmission in China was delineated across three phases, characterized by an initial rapid spread, a subsequent westward expansion, and a final plateau phase with a southwestward trend. The key factors influencing ASF transmission included the pig population density, wind speeds, altitude, and the presence of high-risk roads, with seasonality also playing a significant role in the disease incidence.</p><p><strong>Conclusions and relevance: </strong>This research highlights the significant impact of small-scale farming practices and the live pig transportation network on ASF spread. In addition, it identifies key epidemiological factors, including the pig density and seasonal variations, which are crucial for developing effective disease control strategies.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 4","pages":"e44"},"PeriodicalIF":1.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12329065/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flufenamic acid reduces alveolar bone loss and pyrazole 3 enhances alveolar bone recovery in periodontitis mice.","authors":"Ae Ri Kim, Aeryun Kim, Yu-Mi Yang, Yun-Jung Yoo, Eun-Jung Bak","doi":"10.4142/jvs.24344","DOIUrl":"10.4142/jvs.24344","url":null,"abstract":"<p><strong>Importance: </strong>Transient receptor potential canonical 6 (TRPC6) and TRPC3 are involved in bone remodeling and other biological processes.</p><p><strong>Objective: </strong>To investigate the effects of TRPC6 activator, flufenamic acid (FFA), and TRPC3 inhibitor, pyrazole 3 (PYR), in human periodontal ligament (hPDL) cells and periodontitis mice.</p><p><strong>Methods: </strong>The effects of FFA and PYR on osteoblastogenesis were evaluated in hPDL cells. To investigate periodontitis induction, mice were randomized to control (C), periodontitis (P), and FFA-treated periodontitis (P+FFA) groups. To investigate periodontitis recovery, mice were randomized to day 0 C (D0C), D0P, D3P, D3P+PYR, D7P, and D7P+PYR groups. Alveolar bone (AB) area, osteoclast formation, osteoid area, and Runt-related transcription factor 2 (RUNX2) and receptor activator of nuclear factor-κB ligand (RANKL) expression were evaluated.</p><p><strong>Results: </strong>AB area was greater in the P+FFA group than in the P group, whereas the number of osteoclasts and RANKL expression were lower. AB and osteoid areas were larger in the D7P+PYR group than in the D7P group. RUNX2-positive osteoblasts were elevated in the D3P+PYR group compared to the D0C and D0P groups. Osteocalcin expression was significantly greater on D28 of osteoblast differentiation in hPDL cells in the PYR group compared to the differentiation group.</p><p><strong>Conclusions and relevance: </strong>These results suggest that FFA attenuates AB loss by inhibiting RANKL expression and osteoclast formation and that PYR contributes to AB recovery by enhancing new bone formation.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 4","pages":"e47"},"PeriodicalIF":1.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12329063/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNA sequencing of <i>Mycoplasma bovis</i> infecting bovine mammary epithelial cells and bovine mononuclear cells.","authors":"Noriko Imaizumi, Satoshi Gondaira, Tomochika Sugiura, Ayako Eguchi, Koji Nishi, Jumpei Fujiki, Hidetomo Iwano, Hidetoshi Higuchi","doi":"10.4142/jvs.24347","DOIUrl":"10.4142/jvs.24347","url":null,"abstract":"<p><strong>Importance: </strong><i>Mycoplasma bovis</i> is the major pathogen of bovine mycoplasmosis, such as mastitis, pneumonia, otitis media, and arthritis. Understanding the pathophysiology of mycoplasmal infections from the pathogen-side is as important as elucidating the host-side effects; however, few studies have focused on the variations in the expression of <i>M. bovis</i> genes.</p><p><strong>Objective: </strong>In this study, we performed RNA sequencing of <i>M. bovis</i> infecting bovine mammary epithelial cells (bMECs) and mononuclear cells (MNCs) to elucidate pathogen-side factors involved in the pathophysiology of <i>M. bovis</i> mastitis.</p><p><strong>Methods: </strong><i>M. bovis</i> was cocultured with bMEC and MNC using a transwell system, and total RNA was extracted for RNA sequencing analysis.</p><p><strong>Results: </strong>We identified 162 differentially expressed genes (DEGs), 104 upregulated and 58 downregulated, in <i>M. bovis</i> infecting bMEC, whereas 220 DEGs, 154 upregulated and 66 downregulated, in <i>M. bovis</i> infecting MNC. Enrichment analysis of upregulated DEGs in <i>M. bovis</i> infecting bMEC revealed multiple pathways with overlapping genes involved in lipid metabolism. The expression of six virulence genes were significantly upregulated in <i>M. bovis</i> infecting bMEC and MNC, whereas the expression of three virulence genes were significantly downregulated in <i>M. bovis</i> infecting MNC.</p><p><strong>Conclusions and relevance: </strong>Our findings indicate that <i>M. bovis</i> exhibits infecting cell-specific gene expression, and its virulence varies with the infecting cell type.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":" ","pages":"e42"},"PeriodicalIF":1.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12329052/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of localized cryotherapy on interdigital pruritus of dogs with canine atopic dermatitis.","authors":"Jae-Il Han, Dongkuk Yoo, Eunjoo Kim, Eun-Soo Lee, Yoonseok Jang, Aryung Nam, Jae-Eun Hyun","doi":"10.4142/jvs.25049","DOIUrl":"10.4142/jvs.25049","url":null,"abstract":"<p><strong>Importance: </strong>Interdigital dermatitis represents a significant clinical manifestation in dogs with atopic dermatitis. Given the refractory nature of these lesions to conventional therapies, there is an increasing demand for novel treatment modalities for atopic interdigital dermatitis.</p><p><strong>Objective: </strong>This study aimed to evaluate the efficacy and safety of a novel focal cryotherapy device in improving lesions and clinical symptoms in dogs with atopic interdigital dermatitis.</p><p><strong>Methods: </strong>Twenty dogs diagnosed with atopic interdigital dermatitis were enrolled in this study. Cryotherapy was applied at -5°C for 10 sec to the interdigital spaces. The treatment protocol consisted of three sessions, with 9 dogs receiving weekly treatments and 11 dogs receiving treatments twice weekly. At each visit, a modified Canine Atopic Dermatitis Extent and Severity Index (m-CADESI) and a modified Pruritus Visual Analog Scale (m-PVAS) were employed. Moreover, owner satisfaction and potential adverse effects were evaluated.</p><p><strong>Results: </strong>Both treatment groups showed improvement in erythema and m-CADESI scores post-treatment. However, a statistically significant reduction in m-PVAS was only observed in treatment group 2. Remarkably, patients with baseline m-CADESI scores ≤ 12 showed significantly greater improvement in m-PVAS compared to those with scores > 12. No significant adverse effects were observed in any of the patients, and owner satisfaction was reported as good to excellent.</p><p><strong>Conclusions and relevance: </strong>Focal cryotherapy for atopic interdigital dermatitis, when properly indicated, may be considered a novel, safe, and effective treatment option for reducing lesions and pruritus in affected dogs.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":" ","pages":"e43"},"PeriodicalIF":1.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12329056/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Baicalin confers neuroprotection in animal models of stroke through its antioxidant and anti-apoptotic effects.","authors":"Hyun-Kyoung Son, Dong-Ju Park, Ju-Bin Kang, Phil-Ok Koh","doi":"10.4142/jvs.25055","DOIUrl":"10.4142/jvs.25055","url":null,"abstract":"<p><strong>Importance: </strong>Ischemic stroke leads to neuronal cell death due to a lack of oxygen and glucose. Baicalin is a flavonoid that has antioxidant and anti-inflammatory properties.</p><p><strong>Objective: </strong>The aim of this study is to elucidate the anti-oxidant and anti-apoptotic effects of baicalin in animal models of stroke.</p><p><strong>Methods: </strong>Vehicle or baicalin (100 mg/kg) was administered intraperitoneally immediately after the middle cerebral artery occlusion (MCAO) surgery. Neurobehavioral tests were conducted 24 h post-MCAO and brain tissue was isolated to assess histopathological changes and apoptosis-associated protein expression. Additionally, reactive oxygen species (ROS) and lipid peroxidation (LPO) assays were performed to evaluate oxidative stress.</p><p><strong>Results: </strong>MCAO animals exhibited severe neurological deficits, which were significantly alleviated by baicalin treatment. Baicalin mitigated the up-regulation in ROS and LPO levels induced by surgery. MCAO damage led to severe histopathological lesions and an increase in terminal deoxynucleotidyl transferase dUTP nick end labeling-positive reactions, these alterations were alleviated by baicalin treatment. MCAO damage decreases the expression of Bcl-2 and increases the expression of Bax, baicalin alleviates these changes. Baicalin also attenuated the upregulation of caspase-3 expression caused by MCAO injury.</p><p><strong>Conclusions and relevance: </strong>These results can suggest evidence that baicalin exerts neuroprotective effects by preventing apoptosis during cerebral ischemia. In conclusion, baicalin acts as a potent neuroprotective agent through its antioxidant and anti-apoptotic effects on neuronal cell damage.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":" ","pages":"e37"},"PeriodicalIF":1.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12329058/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}