Journal of Veterinary Science最新文献

{"title":"Choosing the right reporting guideline in veterinary research: an editorial for authors and editors.","authors":"Pan Dong Ryu","doi":"10.4142/jvs.26903","DOIUrl":"10.4142/jvs.26903","url":null,"abstract":"","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"27 5","pages":"e76"},"PeriodicalIF":1.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13655553/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149136824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Measurement-based dosimetric comparison of kilovoltage and megavoltage X-ray systems for static-field 3D conformal radiotherapy in companion animals.","authors":"Yunji Lim, Geon Oh, Sun-Young Kim, Dongho Shin, Jaehwan Lee, Tae Hyun Kim, Sung Ho Moon, YoungMin Yun, Dong-Seok Shin","doi":"10.4142/jvs.26032","DOIUrl":"10.4142/jvs.26032","url":null,"abstract":"<p><strong>Importance: </strong>Kilovoltage (kV) X-ray radiotherapy systems have been introduced as a potential treatment option for companion animal radiotherapy, particularly for superficial targets. On the other hand, the lack of experimental validation of dose delivery accuracy has limited their broader clinical adoption.</p><p><strong>Objective: </strong>A measurement-based dosimetric comparison was made between kV and megavoltage (MV) X-ray systems.</p><p><strong>Methods: </strong>A 300 kV X-ray radiotherapy system and a 6 MV linear accelerator were evaluated for percent depth dose, penetration depth, beam profiles, and field size output factors. Eight static-field plans were generated for targets at varying depths and tissue densities in an anthropomorphic head phantom. Point doses were measured with thermoluminescent dosimeters and compared with treatment planning system-calculated doses. The calculated-to-measured absolute percentage differences were compared between the kV and MV systems using the Wilcoxon signed-rank test with a bootstrap 95% confidence interval.</p><p><strong>Results: </strong>Compared with the MV beam, the kV X-ray beam exhibited a negligible buildup distance, higher field-size dependence, a pronounced heel effect, and substantially lower penetration depth. Despite these dissimilarities, the absolute differences between the calculated and measured point doses were 0.1%-4.0% and 3.1%-6.5% for the kV and MV systems, respectively, across different target depths and tissue densities. The differences were significantly smaller for kV (median paired difference, -1.9 percentage points; 95% CI, -3.8 to -0.7; <i>p</i> = 0.0078).</p><p><strong>Conclusions and relevance: </strong>Under the evaluated static-field phantom conditions, the commissioned kV treatment-planning model showed calculated-to-measured differences of 0.1%-4.0%. Although these values were lower than those for MV, this finding does not establish higher dosimetric accuracy for the kV system because measurement-related uncertainties may have contributed.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":" ","pages":"e60"},"PeriodicalIF":1.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13655565/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148865514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sudden death with pseudomembranous fibrinonecrotic enteritis in a zoo-housed hippopotamus (<i>Hippopotamus amphibius</i>): predominant isolation and genomic characterization of <i>Clostridium perfringens</i> type A.","authors":"Thach Phan Van, Byungkwan Oh, Hadeer Khaled, Hyun Jong Park, Jaime C Cabarles, Chae Woong Lim, Bumseok Kim, John Hwa Lee, Sang-Ik Oh","doi":"10.4142/jvs.26036","DOIUrl":"10.4142/jvs.26036","url":null,"abstract":"<p><strong>Importance: </strong><i>Clostridium perfringens</i> type A (CPA) is a common intestinal commensal, but it has been implicated in peracute fatal enteritis under predisposing conditions. Although clostridial enteric disease is well characterized in domestic species, reports in captive megaherbivores are rare. In addition, genomic data for CPA isolates from zoo-housed wildlife remain scarce, limiting comparative epidemiologic investigations.</p><p><strong>Case presentation: </strong>A 40-year-old female zoo-housed hippopotamus (<i>Hippopotamus amphibius</i>) was found dead without prior clinical signs. Necropsy revealed diffuse pseudomembranous fibrinonecrotic and hemorrhagic enteritis. Histopathology showed acute mucosal necrosis with an overlying fibrinonecrotic pseudomembrane and abundant intralesional bacilli. Aerobic culture yielded <i>Escherichia coli</i> without major virulence genes, whereas anaerobic culture yielded abundant β-hemolytic colonies. Multiplex toxinotyping polymerase chain reaction identified the predominant anaerobe as CPA (<i>plc</i>-positive only). Whole-genome sequencing yielded a 3.25-Mb draft assembly comprising 37 putative chromosomal contigs (3.20 Mb total) and one 48-kb putative plasmid contig. <i>In silico</i> screening detected <i>plc</i>, <i>pfoA</i>, <i>colA</i>, <i>cloSI</i>, multiple sialidase/hexosaminidase genes, and predicted antimicrobial resistance determinants [<i>mprF</i> and <i>tetA(P</i>)/<i>tetB(P)</i>].</p><p><strong>Conclusions and relevance: </strong>To our knowledge, this is the first genomic characterization of a CPA isolate from an adult hippopotamus, providing a reference for zoo-based surveillance and outbreak investigations. Clostridial-type fibrinonecrotic-hemorrhagic enteritis should be considered in the differential diagnosis of sudden death in adult zoo-housed hippopotami.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":" ","pages":"e62"},"PeriodicalIF":1.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13655572/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148864964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-body computed tomography patterns of dystrophic and metastatic soft-tissue calcification in dogs: a multicenter retrospective study.","authors":"Hearang Lim, Sunghoon Jeon, Jinhwa Chang, Kwangyong Yoon, Byungjin Kim, Ji-Young Ban, Dongwoo Chang, Hee-Jung Jee, Namsoon Lee","doi":"10.4142/jvs.25284","DOIUrl":"10.4142/jvs.25284","url":null,"abstract":"<p><strong>Importance: </strong>Differentiating between dystrophic and metastatic soft tissue calcifications in dogs is clinically essential but remains diagnostically challenging.</p><p><strong>Objective: </strong>This retrospective study aimed to categorize canine soft tissue calcifications on whole-body computed tomography (CT) based on anatomical location and pattern, determine if CT can differentiate between metastatic and dystrophic types based on these factors, and identify pattern tendencies within dystrophic subtypes.</p><p><strong>Methods: </strong>Calcifications were classified into group 1 (dystrophic) and group 2 (metastatic). Multivariable generalized estimating equation (GEE) logistic regression identified variables independently associated with metastatic calcification. Group 1 was subdivided into 1a (with mass) and 1b (without mass) to assess pattern tendencies across organs.</p><p><strong>Results: </strong>Organ location alone was not independently associated with calcification type. Instead, similar organ-specific patterns were observed across etiologic groups, indicating substantial topographic overlap; both groups 1b and 2 predominantly exhibited round/oval multifocal patterns in kidneys, and curvilinear/rim diffuse patterns in the vasculature. Conversely, group 1a showed a higher propensity for amorphous/irregular patterns within masses. In multivariable GEE analysis, focal distribution was significantly associated with metastatic calcification (adjusted odds ratio [OR], 1.18; 95% confidence interval [CI], 1.02-1.37; <i>p</i> = 0.029) compared to multifocal distribution, while curvilinear/rim shapes showed a curvilinear/rim shape was not significantly associated with metastatic calcification after adjustment (OR, 1.26; 95% CI, 0.98-1.63; <i>p</i> = 0.072) compared to round/oval shapes.</p><p><strong>Conclusions and relevance: </strong>Whole-body CT effectively categorizes the locations and patterns of canine soft tissue calcifications and identifies distinct tendencies within dystrophic subtypes. However, due to significant organ-specific topographic overlaps, CT imaging alone is insufficient to definitively differentiate metastatic from dystrophic calcifications, necessitating comprehensive clinical and biochemical correlation.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":" ","pages":"e64"},"PeriodicalIF":1.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13655570/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148865069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico characterization of ferritin 2 conserved motifs and B-cell epitopes in <i>Hyalomma anatolicum</i>.","authors":"Xueqing Zhao, Zeyun Cui, Na Zhou, Yuqian Deng, Licui Wen, Wenying Dang, Caishan Li, Bayinchahan Gailike","doi":"10.4142/jvs.25152","DOIUrl":"10.4142/jvs.25152","url":null,"abstract":"<p><strong>Importance: </strong>Ferritin 2 (Fer2) is a well-established candidate antigen for anti-tick vaccines, but few studies have investigated the potential of <i>Hyalomma anatolicum</i> Fer2 as an antigenic peptide.</p><p><strong>Objective: </strong>To characterize the <i>H. anatolicum</i> fer 2 coding sequence, conserved motifs, predicted structure, and linear B-cell epitopes.</p><p><strong>Methods: </strong>The complete coding sequence region of the <i>H. anatolicum</i> Fer2 gene was amplified by polymerase chain reaction. The biological properties and effective antigenic peptide of <i>H. anatolicum</i> Fer2 protein were analyzed.</p><p><strong>Results: </strong>The Fer2 sequence of <i>H. anatolicum</i> collected showed the highest similarity to that of <i>H. anatolicum</i> from Turpan, Xinjiang (accession No. ALJ92580.1), and the phylogenetic analysis accurately classified Fer2 among the different tick species. The biological property results showed that <i>H. anatolicum</i> Fer2 is a non-transmembrane protein with a signal peptide and a typical Fer protein structure. Motif analysis showed that different tick 5 motifs were in random combinations, but motifs 1, 2, and 4 were contained in all tick Fer2 proteins. Correspondence existed between motifs and antigens, with motif 1 containing the highest-scoring antigenic epitope.</p><p><strong>Conclusions and relevance: </strong><i>H. anatolicum</i> fer 2 motif 1 contains a conserved, high-scoring predicted linear B-cell epitope and warrants experimental immunogenicity and protection studies before consideration as an anti-tick vaccine peptide.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"27 5","pages":"e56"},"PeriodicalIF":1.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13655566/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149137187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological study of nasal diverticulum in Grant's zebra (<i>Equus quagga boehmi</i> Matschie, 1892).","authors":"Young-Jin Jang, Dongchoon Ahn","doi":"10.4142/jvs.25372","DOIUrl":"10.4142/jvs.25372","url":null,"abstract":"<p><strong>Importance: </strong>The nasal diverticulum (ND), a blind cutaneous pouch extending from the nasal vestibule (NV), is unique to Equidae. Although anatomical and histological descriptions of the ND are available for horses and donkeys, detailed studies in zebras remain limited.</p><p><strong>Objective: </strong>To investigate and characterize the anatomical and histological features of the ND in zebras and compare these findings with those observed in other species.</p><p><strong>Methods: </strong>Silicone casts of the NDs were prepared from two of three female Grant's zebra carcasses for anatomical measurements, followed by dissection for gross morphological examination. Three-dimensional reconstructions generated from computed tomography images of the casts were used to obtain morphological measurements. In addition, paraffin-embedded sections stained with hematoxylin and eosin were prepared for histological evaluation and morphometric analysis.</p><p><strong>Results: </strong>The NDs of zebras were curved blind pouches, slightly compressed mediolaterally, and extended over the nasal process of the incisive bone. Based on the impression of the nasal process, the ND was divided into cranial and caudoventral regions. The nasolacrimal orifice was located on the lateral aspect of the nostril. An undulating fold was observed on the medial wall of the cranial region of the ND. The total volume of the ND was approximately 36 mL. The surface was covered with fine hairs associated with secondary hair follicles. Compared with the NV, the ND exhibited significantly reduced epidermal thickness and fewer sebaceous glands, sweat glands, and secondary hair follicles.</p><p><strong>Conclusions and relevance: </strong>This study provides novel anatomical and histological data on the ND in zebras, highlighting species-specific differences compared with other animals.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":" ","pages":"e50"},"PeriodicalIF":1.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13655569/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148865530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unilateral congenital testicular seminoma in a neonatal Holstein calf: gross, histopathological, and immunohistochemical characterization.","authors":"Seongmo Yang, Sanggu Kim, Moochang Park, Soochong Kim","doi":"10.4142/jvs.26112","DOIUrl":"https://doi.org/10.4142/jvs.26112","url":null,"abstract":"<p><strong>Importance: </strong>This case report presents a rare case of unilateral congenital testicular seminoma in a neonatal Holstein calf.</p><p><strong>Case presentation: </strong>A neonatal male Holstein calf was presented with a unilaterally enlarged inguinal mass, which was identified immediately after birth. The calf was euthanized at the farmer's request, and both testes were collected for pathological examination. Gross examination of the excised testes revealed a right-sided unilateral testicular neoplasm with a normal contralateral testis. The cut surface was gray to pink with multifocal hemorrhage, congestion, and cystic degeneration. Histologically, the mass was lobulated by fibrous trabeculae, and neoplastic cells were round to oval with scant clear cytoplasm. Notable features included prominent nucleoli, clumped chromatin, severe anisocytosis and anisokaryosis, and a total of 18 mitotic figures counted across 10 high-power fields (HPFs) (18 mitoses/10HPFs; mean, 1.8 mitoses/HPF). Histological findings suggested seminoma as an initial diagnosis. However, the tumor also showed distinct microcystic patterns, which prompted the consideration of yolk sac tumor as a differential diagnosis. Immunohistochemically, the neoplastic cells showed cytoplasmic positivity for CD117, whereas pancytokeratin was negative, confirming the diagnosis of seminoma.</p><p><strong>Conclusions and relevance: </strong>To the best of our knowledge, this is the first case of unilateral congenital seminoma in a neonatal Holstein calf.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149111591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological identification of <i>Dendritobilharzia</i> sp. (Schistosomatidae) from a Whooper Swan in the Republic of Korea.","authors":"Heon Woo Lee, Hyeon-Cheol Kim, Sangjin Ahn, Seongjun Choe, Bae-Keun Park","doi":"10.4142/jvs.26070","DOIUrl":"https://doi.org/10.4142/jvs.26070","url":null,"abstract":"<p><strong>Importance: </strong>Avian schistosomes are important wildlife parasites, but their presence in the Republic of Korea has not been documented in the peer-reviewed literature. Documenting new host and geographic records is essential for understanding the parasite distribution and host-parasite interactions.</p><p><strong>Objective: </strong>To describe the morphology of schistosomes recovered from a Whooper Swan (<i>Cygnus cygnus</i>) and determine their taxonomic identity.</p><p><strong>Methods: </strong>Two male schistosomes were recovered from the aorta of a Whooper Swan during a necropsy. The specimens were fixed, stained with Semichon's acetocarmine, and examined using optical microscopy. Identification was based on the established taxonomic keys. No statistical analysis was performed; all measurements are reported as the observed ranges from the two specimens examined.</p><p><strong>Results: </strong>The specimens showed the diagnostic features of <i>Dendritobilharzia</i>, including the absence of oral and ventral suckers, a flattened body, dendritic intestinal caeca, and numerous tests. Species-level identification was not possible because of the overlapping morphology and the absence of female, larval, and molecular data. The specimens were identified as <i>Dendritobilharzia</i> sp.</p><p><strong>Conclusions and relevance: </strong>This paper reports the first record of <i>Dendritobilharzia</i> from an avian host in the Republic of Korea and provides baseline data on the avian schistosome distribution. These findings improve the understanding of parasite diversity and host-parasite relationships and support future studies on wildlife health and transmission.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149111514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activation of the unfolded protein response via PERK-ATF4 signalling in deoxynivalenol- and PRRSV-co-exposed MARC-145 cells.","authors":"Shaosan Zhang, Jiahui Mi, Meng Li, Xinke Wang, Jiaxing Li, Ding Zhang, Bo Yang, Zilong Sun, Ruiyan Niu","doi":"10.4142/jvs.25161","DOIUrl":"https://doi.org/10.4142/jvs.25161","url":null,"abstract":"<p><strong>Importance: </strong>Deoxynivalenol (DON) is a prevalent mycotoxin that is highly toxic to animals, particularly pigs. Porcine reproductive and respiratory syndrome virus (PRRSV) is a major swine pathogen with high infectivity and severe clinical outcomes, but the mechanisms underlying the host cell responses to co-exposure of DON and PRRSV remain unclear.</p><p><strong>Objective: </strong>This study examined the effects of the co-exposure of Marc-145 cells with DON and PRRSV.</p><p><strong>Methods: </strong>A DON- and PRRSV-co-exposed MARC-145 cells model was established, and transcriptomic/proteomic sequencing combined with Western blot and immunofluorescence assays explored the mechanism disrupting microenvironmental homeostasis.</p><p><strong>Results: </strong>Integrated transcriptomic and proteomic analyses showed that the PRRSV infection and DON co-exposure enriched four molecular function categories in Gene Ontology and one Kyoto Encyclopedia of Genes and Genomes pathway. Protein-protein interaction network analysis identified key hub proteins that were central to the unfolded protein response (UPR) and endoplasmic reticulum stress: glucose-regulated protein 78 (GRP78), activating transcription factor 6 beta (ATF6b), inositol-requiring enzyme 1α (IRE1α), activating transcription factor 4 (ATF4), and protein kinase R-like endoplasmic reticulum kinase (PERK). Experimental validation showed that co-exposure elevated the ATF6b, phosphorylated PERK, and phosphorylated eukaryotic initiation factor-2α levels, while reducing GRP78, IRE1α, ATF6b nuclear form, ATF4, and growth arrest and DNA damage-inducible protein 34, thereby activating the UPR.</p><p><strong>Conclusions and relevance: </strong>Ultimately, co-exposure to DON and PRRSV disrupted the intracellular microenvironment in Marc-145 cells by modulating the PERK-ATF4 signaling pathway, accompanied by reducing the ability of host cells to replicate the virus.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149111597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic sequencing reveals critical role of STING pathway in PPRV-infected goat endometrial epithelial cells.","authors":"Mengyuan Liu, Yaqi Chen, Yixian Guo, Xiaoge Wang, Minrui Wu, Xiaozhu Yang, Chenyang Wang, Hanwei Yin, Bo Wen, Hongnuan Wang, Zilong Sun, Ding Zhang, Jingyu Wang, Shaopeng Gu, Bo Yang, Wei Li","doi":"10.4142/jvs.25348","DOIUrl":"https://doi.org/10.4142/jvs.25348","url":null,"abstract":"<p><strong>Importance: </strong>Peste des petits ruminants virus (PPRV) is the causative agent of peste des petits ruminants (PPR), a highly contagious disease in small ruminants that causes massive economic losses worldwide. Despite its impact, the mechanisms underlying PPRV infections remain elusive, hindering its control and prevention.</p><p><strong>Objective: </strong>This study examined the molecular mechanisms underlying PPRV infections by investigating the transcriptomic changes in PPRV-infected goat endometrial epithelial cells (EECs) and clarifying the regulatory role of the stimulator of interferon genes (STING) signaling pathway in PPRV replication.</p><p><strong>Methods: </strong>Transcriptome sequencing was performed on EECs at 72 h after a PPRV infection. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were conducted on the identified differentially expressed genes (DEGs). Western blot analysis was used to detect STING and p-TBK1 expression in various cell lines at different time points after the PPRV infection. The effects of STING on PPRV replication were evaluated by STING overexpression and knockdown using quantitative real-time polymerase chain reaction, immunofluorescence, and 50% tissue culture infectious dose (TCID₅₀) assay.</p><p><strong>Results: </strong>A total of 1946 DEGs (1,078 up-regulated and 868 down-regulated) were identified in PPRV-infected EECs. GO and KEGG enrichment analysis showed that the DEGs were significantly enriched in terms related to immune response. Western blot analysis showed that PPRV promotes STING and p-TBK1 expression. Specifically, overexpression of STING significantly enhanced PPRV-N protein levels (<i>p</i> < 0.05) and viral titers (<i>p</i> < 0.05) in Vero CCL-81 cells and glomerular microvascular endothelial cells, whereas knockdown of STING decreased viral protein expression (<i>p</i> < 0.05) and viral titers (<i>p</i> < 0.05) in these cells.</p><p><strong>Conclusions and relevance: </strong>A PPRV infection activates the STING pathway, which facilitates viral replication. This finding helps reveal the pathogenesis of PPRV and provides a theoretical basis for the prevention and control of PPR.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149111573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}