Journal of Veterinary Science最新文献

{"title":"Genomic insights of <i>S. aureus</i> associated with bovine mastitis in a high livestock activity region of Mexico.","authors":"José Roberto Aguirre-Sánchez, Nohemí Castro-Del Campo, José Andrés Medrano-Félix, Alex Omar Martínez-Torres, Cristóbal Chaidez, Jordi Querol-Audi, Nohelia Castro-Del Campo","doi":"10.4142/jvs.23286","DOIUrl":"10.4142/jvs.23286","url":null,"abstract":"<p><strong>Importance: </strong>Bovine mastitis, predominantly associated with gram-positive <i>Staphylococcus aureus</i>, poses a significant threat to dairy cows, leading to a decline in milk quality and volume with substantial economic implications.</p><p><strong>Objective: </strong>This study investigated the incidence, virulence, and antibiotic resistance of <i>S. aureus</i> associated with mastitis in dairy cows.</p><p><strong>Methods: </strong>Fifty milk-productive cows underwent a subclinical mastitis diagnosis, and the <i>S. aureus</i> strains were isolated. Genomic DNA extraction, sequencing, and bioinformatic analysis were performed, supplemented by including 124 <i>S. aureus</i> genomes from cows with subclinical mastitis to enhance the overall analysis.</p><p><strong>Results: </strong>The results revealed a 42% prevalence of subclinical mastitis among the cows tested. Genomic analysis identified 26 sequence types (STs) for all isolates, with Mexican STs belonging primarily to CC1 and CC97. The analyzed genomes exhibited multidrug resistance to phenicol, fluoroquinolone, tetracycline, and cephalosporine, which are commonly used as the first line of treatment. Furthermore, a similar genomic virulence repertoire was observed across the genomes, encompassing the genes related to invasion, survival, pathogenesis, and iron uptake. In particular, the toxic shock syndrome toxin (<i>tss-1</i>) was found predominantly in the genomes isolated in this study, posing potential health risks, particularly in children.</p><p><strong>Conclusion and relevance: </strong>These findings underscore the broad capacity for antibiotic resistance and pathogenicity by <i>S. aureus</i>, compromising the integrity of milk and dairy products. The study emphasizes the need to evaluate the effectiveness of antibiotics in combating <i>S. aureus</i> infections.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11291432/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141442970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determining genetic diversity of prevalent G and P genotype of <i>Bovine Rotavirus A</i> from neonatal calves of Gujarat, India.","authors":"Akash Golaviya, Rafiyuddin Mathakiya, Subhash Jakhesara, Prakash Koringa","doi":"10.4142/jvs.24124","DOIUrl":"10.4142/jvs.24124","url":null,"abstract":"<p><strong>Importance: </strong>Neonatal calf diarrhea is a major cause of mortality in newborn calves worldwide, posing a significant challenge in bovine herds. <i>Group A Bovine Rotaviruses</i> (<i>BRVA</i>) are the primary contributors to severe gastroenteritis in calves under two months old.</p><p><strong>Objectives: </strong>This study examined the prevalence and molecular characterization of <i>BRVA</i> in neonatal calves in Gujarat, India.</p><p><strong>Methods: </strong>Sixty-nine diarrheic fecal samples were collected and subjected to various molecular methods of <i>BRVA</i> detection, isolation, and characterization.</p><p><strong>Results: </strong>The latex agglutination test (LAT), electropherotyping (RNA-PAGE), and reverse transcription polymerase chain reaction revealed positivity rates of 39.13%, 20.30%, and 37.70%, respectively. RNA-PAGE identified 11 bands with a 4:2:3:2 migration pattern, indicative of the segmented genome of <i>BRVA</i>. <i>BRVA</i> was successfully isolated from LAT-positive samples, with 26 samples exhibiting clear cytopathic effects upon passage in MA-104 cell lines. Genotyping identified G10 as the predominant G genotype, with P[11] genotypes comprising 76.92% of the isolates. The most common G/P combination was G10P[11], highlighting its zoonotic potential.</p><p><strong>Conclusions and relevance: </strong>These findings underscore the importance of molecular detection and genotyping for effective vaccine development. This study provides crucial insights into the prevalent G and P genotypes of <i>BRVA</i> in Gujarat, India, aiding in the development of targeted control measures.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11291431/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacokinetics of oxytetracycline in hybrid catfish (<i>Clarias macrocephalus</i> x <i>C. gariepinus</i>) after intravascular and oral administrations.","authors":"Amnart Poapolathep, Kednapat Sriphairoj, Sittichai Hatachote, Kannika Wongpanit, Duangkamol Saensawath, Narumol Klangkaew, Napasorn Phaochoosak, Mario Giorgi, Saranya Poapolathep","doi":"10.4142/jvs.24118","DOIUrl":"10.4142/jvs.24118","url":null,"abstract":"<p><strong>Importance: </strong>Over the past decade, catfish farming has increased in Southeast Asia. However, there has been no existing for pharmacokinetic data in the hybrid catfish (<i>Clarias macrocephalus</i> x <i>C. gariepinus</i>).</p><p><strong>Objective: </strong>This study was designed to evaluate the pharmacokinetic characteristics of oxytetracycline (OTC) in the hybrid catfish, following single intravascular (IV) or oral (PO) administration at a single dosage of 50 mg/kg body weight (BW).</p><p><strong>Methods: </strong>In total, 140 catfish (each about 100-120 g BW) were divided into two groups (n = 70). Blood samples (0.6-0.8 mL) were collected from ventral caudal vein at pre-assigned times up to 144 h (sparse samples design). OTC plasma concentrations were analyzed using high-performance liquid chromatography-photodiode array detector.</p><p><strong>Results: </strong>The pharmacokinetic parameter of OTC was evaluated using a non-compartment model. OTC plasma concentrations were detectable for up to 144 and 120 h after IV and PO, respectively. The elimination half-life value of OTC was long with slow clearance after IV administration in hybrid catfish. The average maximum concentration value of OTC was 2.72 µg/mL with a time at the maximum concentration of 8 h. The absolute PO bioavailability was low (2.47%).</p><p><strong>Conclusions and relevance: </strong>These results showed that PO administration of OTC at a dosage of 50 mg/kg BW was unlikely to be effective for clinical use in catfish. The pharmacodynamic properties and clinical efficacy of OTC after multiple medicated feed are warranted.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11291427/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Minimally traumatic extraction of fractured bilateral maxillary canine teeth using a piezoelectric surgical unit in an African lion (<i>Panthera leo</i>).","authors":"Se Eun Kim, Yoocheol Jeong","doi":"10.4142/jvs.24099","DOIUrl":"10.4142/jvs.24099","url":null,"abstract":"<p><strong>Importance: </strong>Canine extraction of large carnivores can pose significant risk due to extensive tissue damage during aggressive bone reduction. This report highlights a rare instance in which the use of a piezoelectric surgical unit (PSU) for maxillary canine extraction in a large carnivore resulted in successful outcomes with minimal tissue damage.</p><p><strong>Case presentation: </strong>A 10-year-old male African lion presented with decreased appetite because of bilateral maxillary canine fractures. Intraoral radiographs revealed enlarged root canals and periapical radiolucency of the fractured canines, leading to a diagnosis of periapical periodontitis and pulpitis. To extract the right maxillary canine, conventional method using hand instrument failed to achieve adequate luxation, necessitating the use of the flat blade of the PSU to sever the periodontal ligament. The left maxillary canine was extracted using PSU from the beginning, and the extraction time was markedly shortened by using PSU without additional alveolar bone damage or bleeding.</p><p><strong>Conclusion and relevance: </strong>This case demonstrated that utilizing PSU for canine extraction in a lion resulted in periodontal ligament separation, reducing damage to the alveolar bone and shortening surgical time. It suggests the promising application of PSU in tooth extraction for large wild animals, indicating its potential significance in veterinary dentistry.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11291438/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141442971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brain invasion of bovine coronavirus: molecular analysis of bovine coronavirus infection in calves with severe pneumonia and neurological signs.","authors":"Semaha Gul Yilmaz, Ozge Aydin, Hasan Emre Tali, Gizem Karadag, Kivilcim Sonmez, Erhan Bayraktar, Aysun Yilmaz, Nuri Turan, Zihni Mutlu, Munir Iqbal, Jurgen A Richt, Huseyin Yilmaz","doi":"10.4142/jvs.23261","DOIUrl":"10.4142/jvs.23261","url":null,"abstract":"<p><strong>Importance: </strong>Although the role of bovine coronavirus (BCoV) in calf diarrhea and respiratory disorders is well documented, its contribution to neurological diseases is unclear.</p><p><strong>Objective: </strong>This study conducted virological investigations of calves showing diarrhea and respiratory and neurological signs.</p><p><strong>Methods: </strong>An outbreak of diarrhea, respiratory, and neurological disorders occurred among the 12 calves in July 2022 in Istanbul, Türkiye. Two of these calves exhibited neurological signs and died a few days after the appearance of symptoms. One of these calves was necropsied and analyzed using molecular and histopathological tests.</p><p><strong>Results: </strong>BCoV RNA was detected in the brain, lung, spleen, liver, and intestine of the calf that had neurological signs by real-time reverse transcription polymerase chain reaction. Immunostaining was also observed in the intestine and brain. A 622 bp S1 gene product was noted on gel electrophoresis only in the brain. Phylogenetic analysis indicated that the BCoV detected in this study had a high proximity to the BCoV strain GIb with 99.19% nucleotide sequence homology to the strains detected in Poland, Israel, Türkiye, and France. No distinct genetic lineages were observed when the brain isolate was compared with the respiratory and enteric strains reported to GenBank. In addition, the highest identity (98,72%) was obtained with the HECV 4408 and L07748 strains of human coronaviruses.</p><p><strong>Conclusions and relevance: </strong>The strain detected in a calf brain belongs to the GIb-European lineage and shares high sequence homology with BCoV strains detected in Europe and Israel. In addition, the similarity between the human coronaviruses (4408 and L07748) raises questions about the zoonotic potential of the strains detected in this study.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11291437/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141442969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative proteomic analysis of PK-15 cells infected with wild-type strain and its EP0 gene-deleted mutant strain of pseudorabies virus.","authors":"Di Wang, Dongjie Chen, Shengkui Xu, Fang Wei, Hongyuan Zhao","doi":"10.4142/jvs.24069","DOIUrl":"10.4142/jvs.24069","url":null,"abstract":"<p><strong>Importance: </strong>As one of the main etiologic agents of infectious diseases in pigs, pseudorabies virus (PRV) infections have caused enormous economic losses worldwide. EP0, one of the PRV early proteins (EP) plays a vital role in PRV infections, but the mechanisms are unclear.</p><p><strong>Objective: </strong>This study examined the function of EP0 to provide a direction for its in-depth analysis.</p><p><strong>Methods: </strong>In this study, the EP0-deleted PRV mutant was obtained, and Tandem Mass Tag-based proteomic analysis was used to screen the differentially expressed proteins (DEPs) quantitatively in EP0-deleted PRV- or wild-type PRV-infected porcine kidney 15 cells.</p><p><strong>Results: </strong>This study identified 7,391 DEPs, including 120 and 21 up-regulated and down-regulated DEPs, respectively. Western blot analysis confirmed the changes in the expression of the selected proteins, such as speckled protein 100. Comprehensive analysis revealed 141 DEPs involved in various biological processes and molecular functions, such as transcription regulator activity, biological regulation, and localization.</p><p><strong>Conclusions and relevance: </strong>These results holistically outlined the functions of EP0 during a PRV infection and might provide a direction for more detailed function studies of EP0 and the stimulation of lytic PRV infections.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11291433/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological, and molecular investigation of <i>Canine parvovirus-2</i> infection in Egypt.","authors":"Eman Farag Ammar, Yamen Mohammed Hegazy, Magdy Al-Gaabary, Samah M Mosad, Mohamed Salem, Mohamed Marzok, Fadhel Housawi, Mohamed Al-Ali, Abdulrahman Alhaider, Amin Tahoun","doi":"10.4142/jvs.23270","DOIUrl":"10.4142/jvs.23270","url":null,"abstract":"<p><strong>Importance: </strong>Canine parvovirus enteritis (CPE) is a contagious viral disease of dogs caused by the canine parvovirus-2 (CPV-2) associated with high morbidity and mortality rates. CPV-2 has a high global evolutionary rate. Molecular characterization of CPV-2 and understanding its epidemiology are essential for controlling CPV-2 infections.</p><p><strong>Objective: </strong>This study examined the risk factors and survival outcomes of dogs infected with CPV-2. Molecular characterization of CPV-2 genotypes circulating in Egypt was performed to determine the evolution of CPV-2 nationally and globally.</p><p><strong>Methods: </strong>An age-matched case-control study was conducted on 47 control and 47 CPV-infected dogs. Conditional logistic regression analysis examined the association between the potential risk factors and CPE in dogs. Survival analysis was performed to determine the survival pattern of the infected dogs. Thirteen fecal samples from infected dogs were collected to confirm the CPV genotype by CPV-2 VP2 gene sequencing, assembly of nucleotide sequences, and phylogenic analysis.</p><p><strong>Results: </strong>Unvaccinated and roamer dogs had eight and 2.3 times higher risks of CPV infection than vaccinated dogs and non-roamer dogs, respectively. The risk of death from CPE was high among dogs without routine visits to veterinary clinics and among non-roamer dogs. Molecular characterization of CPV-2 confirmed its genotype identity and relationship with the CPV-2 c and b clade types.</p><p><strong>Conclusions and relevance: </strong>This study highlights the potential factors for CPE control, especially vaccination and preventing dogs from roaming freely outside houses. Isolated CPV genotypes are closely related to southern Asian genotypes, suggesting a substantial opportunity for global transmission.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11291436/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Imaging guided adrenalectomy with Indocyanine green fluorescence in a dog with a pheochromocytoma.","authors":"Seung-Yeon Yu, Sungin Lee","doi":"10.4142/jvs.24146","DOIUrl":"10.4142/jvs.24146","url":null,"abstract":"<p><strong>Importance: </strong>This case report presents the successful surgical removal of a pheochromocytoma in a dog using indocyanine green (ICG) fluorescence imaging.</p><p><strong>Case presentation: </strong>A 10-year-old, 6.87 kg, spayed female poodle was referred for an abdominal mass and eight episodes of vomiting. Computed tomography revealed masses in the spleen and left adrenal gland. Based on the plasma normetanephrine levels, the patient was diagnosed with pheochromocytoma. Subsequently, a total splenectomy and left adrenalectomy were performed. A dose of 5 mg/kg ICG was administered intravenously 24 h prior to surgery. Using ICG allowed visualization of tumor margins, aiding in complete resection and minimizing anesthesia-related risks.</p><p><strong>Conclusions and relevance: </strong>No evidence of recurrence or metastasis has been observed for 12 months. To our knowledge, this is the first report of the surgical removal of a pheochromocytoma using intraoperative ICG imaging in veterinary medicine. This case highlights the efficacy of ICG fluorescence imaging as a valuable tool for the surgical management of adrenal tumors in veterinary medicine.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11291434/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence and risk factors of sheep and goat pox virus in selected districts of Wolaita Zone, Southern Ethiopia.","authors":"Fentaye Kassa, Haben Fesseha, Mesfin Mathewos, Selenat Getachew, Nato Hundessa, Saliman Aliye, Isayas Asefa Kebede","doi":"10.4142/jvs.24086","DOIUrl":"10.4142/jvs.24086","url":null,"abstract":"<p><strong>Importance: </strong>Sheep and goat pox (SGP) virus infection is a highly fatal viral infection of small ruminants that causes major production losses in sheep and goats in Ethiopia while also limiting international trade.</p><p><strong>Objective: </strong>This study aimed to estimate the seroprevalence of SGP infection and assess related risk variables.</p><p><strong>Methods: </strong>A cross-sectional study was conducted from February to August 2023 on 384 serum samples taken from sheep and goats. A serum neutralization test was conducted to detect the presence of antibodies against the SGP virus in Wolaita Sodo Regional Laboratory.</p><p><strong>Results: </strong>The overall seroprevalence rate of SGP was 4.95%. Factors such as sheep (8.26%), female sheep and goats (7.45%), older sheep and goats (8.33%), larger flock size of sheep and goats (10.47%), poorly conditioned sheep and goats (31.58%), sheep and goats with a tick on their skin (10.38%), and animals that had not been vaccinated (5.17%) were found to have higher seroprevalence. Furthermore, the seropositivity in sheep was five times greater than in goats (adjusted odds ratio [AOR], 4.73; 95% confidence interval [CI], 1.39-15.99). Additionally, large-sized flocks of sheep and goats were more likely to be seropositive to pox disease than small-sized flocks (AOR, 6.73; 95% CI, 1.58-28.67).</p><p><strong>Conclusions and relevance: </strong>Thus, the study revealed the prevalence of SGP in the Wolaita zone. Additional research should be conducted to estimate the extent of the disease at the regional level, and management measures should be implemented to reduce the economic losses associated with this condition.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11291429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Salmonella species from poultry slaughterhouses in South Korea: carry-over transmission of <i>Salmonella</i> Thompson ST292 in slaughtering process.","authors":"Yewon Cheong, Jun Bong Lee, Se Kye Kim, Jang Won Yoon","doi":"10.4142/jvs.24053","DOIUrl":"10.4142/jvs.24053","url":null,"abstract":"<p><strong>Importance: </strong><i>Salmonella</i> outbreaks linked to poultry meat have been reported continuously worldwide. Therefore, <i>Salmonella</i> contamination of poultry meats in slaughterhouses is one of the critical control points for reducing disease outbreaks in humans.</p><p><strong>Objective: </strong>This study examined the carry-over contamination of <i>Salmonella</i> species through the entire slaughtering process in South Korea.</p><p><strong>Methods: </strong>From 2018 to 2019, 1,097 samples were collected from the nine slaughterhouses distributed nationwide. One hundred and seventeen isolates of <i>Salmonella</i> species were identified using the <i>invA</i> gene-specific polymerase chain reaction, as described previously. The serotype, phylogeny, and antimicrobial resistance of isolates were examined.</p><p><strong>Results: </strong>Among the 117 isolates, 93 were serotyped into <i>Salmonella</i> Mbandaka (n = 36 isolates, 30.8%), <i>Salmonella</i> Thompson (n = 33, 28.2%), and <i>Salmonella</i> Infantis (n = 24, 20.5%). Interestingly, allelic profiling showed that all <i>S</i>. Mbandaka isolates belonged to the lineage of the sequence type (ST) 413, whereas all <i>S</i>. Thompson isolates were ST292. Moreover, almost all <i>S</i>. Thompson isolates (97.0%, 32/33 isolates) belonging to ST292 were multidrug-resistant and possessed the major virulence genes whose products are required for full virulence. Both serotypes were distributed widely throughout the slaughtering process. Pulsed-field gel electrophoretic analysis demonstrated that seven <i>S</i>. Infantis showed 100% identities in their phylogenetic relatedness, indicating that they were sequentially transmitted along the slaughtering processes.</p><p><strong>Conclusions and relevance: </strong>This study provides more evidence of the carry-over transmission of <i>Salmonella</i> species during the slaughtering processes. ST292 <i>S</i>. Thompson is a potential pathogenic clone of <i>Salmonella</i> species possibly associated with foodborne outbreaks in South Korea.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11156591/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141247505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}