Journal of Veterinary Science最新文献

{"title":"Morphology of subclavian artery branching patterns in cats (<i>Felis catus</i>).","authors":"Young-Jin Jang, Pill Soon Jeong, Hyun-Jin Tae, Byung-Yong Park, In-Shik Kim, Dongchoon Ahn","doi":"10.4142/jvs.25014","DOIUrl":"10.4142/jvs.25014","url":null,"abstract":"<p><strong>Importance: </strong>The subclavian artery (SB) is a crucial vascular structure that supplies the neck, thoracic wall, and forelimb. In carnivores, SB branching patterns exhibit considerable interspecific and intraspecific variation. Despite the increasing recognition of cats as companion animals, detailed studies of feline SB branching patterns remain limited.</p><p><strong>Objective: </strong>We aimed to investigate the SB branching patterns in cats, analyze intraspecific variations, and compare these findings with those observed in other carnivores.</p><p><strong>Methods: </strong>Silicone casts of the SBs were prepared from 35 cats (15 males and 20 females). Branching locations of arterial branches were measured as the distance from the aortic arch to each branching point. The SB branching patterns were analyzed based on the branching relationships among the four major branches: the vertebral artery (VT), costocervical trunk (CCT), superficial cervical artery (SC), and internal thoracic artery (IT).</p><p><strong>Results: </strong>Various branching patterns were observed in the SB with a total of 33 branching types. The main branching patterns were determined to be VT, followed by IT, CCT, and SC. The right SB branches exhibited more distal branching locations from the aortic arch compared to the left SB branches. Across individuals, the VT, CCT, and SC followed a consistent order, whereas the IT varied in position.</p><p><strong>Conclusions and relevance: </strong>This study offers a detailed analysis of the SB branching patterns in cats, highlighting significant intraspecific variation and distinctive branching order. These findings enhance our understanding of vascular diversity among carnivores and provide a strong foundation for future comparative anatomical and developmental investigations.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 3","pages":"e35"},"PeriodicalIF":1.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146025/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological investigation and fiber gene genetic analysis of fowl adenovirus serotype 4 from 2017 to 2021 in Yunnan Province, China.","authors":"Xiaoyan Xue, Zhenxing Zhang, Qinhong Yang, Wei Wang, Jianling Song","doi":"10.4142/jvs.24121","DOIUrl":"10.4142/jvs.24121","url":null,"abstract":"<p><strong>Importance: </strong>Through the epidemiological survey of fowl adenovirus serotype 4 (FAdV-4) in poultry and wild birds in Yunnan Province from 2017 to 2021, this study provides a scientific basis for the prevention and control of FAdV-4 in the region. Currently, there is a lack of monitoring and epidemiological research on FAdV-4 in wild birds in China, offering valuable insights for future studies on the virus in wild populations. Additionally, the analysis of the fiber gene sequence of FAdV-4 identified the prevalent strains in Yunnan Province, which lays the groundwork for future treatment and prevention strategies.</p><p><strong>Objective: </strong>This study aimed to understand the epidemic situation of FAdV-4 in Yunnan Province and provide a reference for the prevention and control of the infection.</p><p><strong>Methods: </strong>Suspected samples of FAdV-4 were collected with the wild birds from overwintering habitat and naturally dying poultry from poultry farms in Yunnan Province, and polymerase chain reaction (PCR) detection of FAdV-4 and related co-infection pathogens, pathogen isolation, and genetic evolution analysis of the <i>fiber</i> gene were carried out.</p><p><strong>Results: </strong>The results showed that the FAdV-4 positive rate in poultry and migratory birds was 11.3% and 0.3%, respectively. The infection rate of the poultry tissue samples was high in central and western Yunnan Province. The detection of common poultry infectious diseases prone to co-infection, it was found that the birds were mainly co-infected with pathogens such as infectious laryngotracheitis, chicken infectious anemia virus, and avian influenza virus. Five poultry-source strains and one wild bird-source strain were isolated, and phylogenetic analysis showed that they all had high homology with the early Chinese highly pathogenic strains and clustered with the same genetic and evolutionary clade as the Chinese reference strain. The fiber protein is characterized by loci that indicate highly pathogenic strains (G219D, P307A, V319I, and A308T).</p><p><strong>Conclusions and relevance: </strong>The results of our study demonstrate that FAdV-4 continues to exhibit a high prevalence rate among poultry populations in Yunnan Province, China. Notably, we report the first detection of FAdV-4 in the feces of black-necked cranes.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 3","pages":"e27"},"PeriodicalIF":1.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146020/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel thrombospondin-1 variant as a potential diagnostic biomarker and therapeutic target in canine mammary tumor and osteosarcoma cells.","authors":"Yukyung Hong, Heyeon Baik, Jeongmin Lee, Won Seok Oh, Junsun Ryu, Seung Joon Baek","doi":"10.4142/jvs.24165","DOIUrl":"10.4142/jvs.24165","url":null,"abstract":"<p><strong>Importance: </strong>Thrombospondin-1 (TSP1) is a vital glycoprotein that plays a key role in critical biological functions, including cell migration, invasion, angiogenesis, and proliferation. Understanding the roles of its variants, particularly TSP1 variant (TSP1V), is critical for cancer biology and therapy.</p><p><strong>Objective: </strong>This study examined the expression of the transcriptional variant TSP1V, focusing on canine TSP1 sequences.</p><p><strong>Methods: </strong>The expression of canine TSP1 sequences was analyzed using a reverse transcription polymerase chain reaction (RT-PCR) to identify the <i>TSP1</i> wild-type (<i>dTSP1W</i>) and novel canine <i>TSP1V</i> transcripts (<i>dTSP1V</i>). The effects of damnacanthal and genistein, anticancer compounds, on the viability of canine mammary and osteosarcoma cell lines were assessed by modulating the expression ratios of <i>TSP1V</i> to <i>TSP1W</i> at the transcriptional level. RT-PCR analysis compared the relative <i>dTSP1V</i> and <i>dTSP1W</i> concentrations in normal and tumor canine mammary tissues.</p><p><strong>Results: </strong>Two <i>dTSP1V</i> were identified. Treatment with damnacanthal and genistein decreased cell proliferation in canine mammary and osteosarcoma cell lines, associated with changes in the <i>TSP1V</i> to <i>TSP1W</i> expression ratio. RT-PCR analysis revealed increased <i>dTSP1V</i> expression in normal tissues, while <i>dTSP1W</i> expression was elevated in tumor tissues.</p><p><strong>Conclusions and relevance: </strong>TSP1V may be a potential diagnostic biomarker and therapeutic target for mammary tumors and osteosarcoma in dogs. The differential expression of <i>dTSP1V</i> and <i>dTSP1W</i> in normal versus tumor tissues underscores the importance of TSPIV in cancer biology, expanding the understanding of its role beyond human thyroid cancer and laying the groundwork for future research in other cancers and species.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 2","pages":"e22"},"PeriodicalIF":1.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11972944/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of acaricidal effect of terpinolene and γ-terpinene on <i>Hyalomma anatolicum</i> and <i>in silico</i> screening of herbs.","authors":"Caishan Li, Licui Wen, Wenyu Shi, Yuqian Deng, Na Zhou, Xueqing Zhao, Ercha Hu, Bayinchahan Gailike","doi":"10.4142/jvs.24196","DOIUrl":"10.4142/jvs.24196","url":null,"abstract":"<p><strong>Importance: </strong>Terpenoids of a plant origin are a class of alternatives to chemical acaricides for tick control. Despite this, few studies have evaluated the efficacy of terpenoid-containing herbal extracts or individual terpenoids in <i>Hyalomma anatolicum</i>.</p><p><strong>Objective: </strong>This study evaluated the killing efficacy of terpinolene and γ-terpinene against <i>H. anatolicum</i> to identify herbs rich in these compounds as potential candidates for future materials in the control of <i>H. anatolicum</i>.</p><p><strong>Methods: </strong>Terpinolene and γ-terpinene were subjected to adult tick immersion experiments to evaluate their killing effect on <i>H. anatolicum</i>, following their identification post-collection. The herbs were screened for the two terpenoids using the HERB database, and the resulting data were categorized and analyzed.</p><p><strong>Results: </strong>The acaricidal effect of terpinolene and γ-terpinene against <i>H. anatolicum</i> showed a clear dose-response relationship, with effective doses LC₅₀ of 6.60 mg/mL and 4.86 mg/mL, respectively. <i>In silico</i> analysis revealed the presence of terpinolene and γ-terpinene in 52 and 36 herbs, respectively, with 16 herbs containing both compounds. These herbs could be grouped into 35 plant families. In particular, Lamiaceae and Apiaceae emerged as the most represented families, each accounting for 9.23% of the total herbs identified, followed by Zingiberaceae (7.69%) and Asteraceae and Rutaceae (6.15% each).</p><p><strong>Conclusions and relevance: </strong>Terpinolene and γ-terpinolene have good killing effects against <i>H. anatolicum</i>. Herbs rich in the two terpenoids are promising candidates as plant-derived materials for managing <i>H. anatolicum</i>.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 2","pages":"e19"},"PeriodicalIF":1.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11972941/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protumor effect of CXCL10/CXCR3 axis in canine mammary gland tumor.","authors":"Soyeon Cho, Na-Yon Kim, Yeseul Yang, Jae-Ha Jung, Dansong Seo, Soyun Jin, Sung-Hyun Hwang, Yongbaek Kim","doi":"10.4142/jvs.24278","DOIUrl":"10.4142/jvs.24278","url":null,"abstract":"<p><strong>Importance: </strong>Chemokines and their receptors play integral roles in carcinogenesis. CXC motif chemokine receptor 3 (CXCR3) in T cells mediates anti-tumor effects, whereas CXCR3 in malignant cells promotes proliferation and metastasis. Although the role of CXCR3 has been well-documented in human cancers, including breast cancer, its function in canine tumors remains largely unexplored.</p><p><strong>Objective: </strong>This study aimed to investigate the effects of CXCR3 and its ligand interaction in canine mammary gland tumor (cMGT) cells.</p><p><strong>Methods: </strong>CXCR3 expression in two cMGT cell lines, CIPp and CIPm, was evaluated using real-time quantitative polymerase chain reaction, western blotting, and flow cytometry. CXC motif chemokine ligand 10 (CXCL10)-induced changes in CXCR3 protein expression in cMGT cells were assessed using membrane fractionation assays. Cell proliferation and migration in response to CXCL10 treatment were analyzed using Cell Counting Kit-8 and wound-healing assays, respectively. Additionally, the downstream molecular mechanisms of the CXCL10/CXCR3 axis were examined.</p><p><strong>Results: </strong>CXCR3 expression was significantly higher in CIPm than in CIPp cells. In both the cMGT cell lines, CXCL10 treatment reduced CXCR3 expression on the cell membrane in a dose- and time-dependent manner. The CXCR10/CXCR3 axis promoted cell proliferation and migration in cMGT cells. CXCL10/CXCR3 interaction upregulated the phosphorylation of AKT1 and ERK.</p><p><strong>Conclusions and relevance: </strong>This study demonstrates that the CXCL10/CXCR3 axis may contribute to the pathogenesis of cMGTs by promoting tumor cell proliferation and migration. CXCR3 signaling represents a potential therapeutic target for cMGTs.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 2","pages":"e18"},"PeriodicalIF":1.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11972938/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of florfenicol on body weight, intestinal inflammatory response, intestinal fluid metabolism and microorganisms in broilers.","authors":"Weiqi Shen, Keyao Wang, Yufeng Han, Huimin Wang, Zhibian Duan","doi":"10.4142/jvs.24194","DOIUrl":"10.4142/jvs.24194","url":null,"abstract":"<p><strong>Importance: </strong>Although antibiotics can prevent and treat diseases, their overuse can be harmful. Despite this, the effects of orally administered florfenicol on intestinal health in broilers are unclear.</p><p><strong>Objective: </strong>This study examined oral florfenicol's effects on intestinal function in broilers to guide its clinical use.</p><p><strong>Methods: </strong>One hundred and twenty Arbor Acres broilers (8-d-old) were divided randomly into the control (N) and florfenicol groups (F). The broilers in group F were fed 100 mg/kg body weight florfenicol for seven days. On day 15, the broilers were euthanized and sampled to analyze fluid metabolism-related genes and proteins, jejunal morphological and microbiota.</p><p><strong>Results: </strong>The results revealed a decrease in body weight and an increase in diarrhea rate in broilers in group F compared to group N. The villus length and villus length/crypt depth (V/C) of the jejunum were lower in group F than in group N, whereas the crypt depth was higher. The levels of tumor necrosis factor α, Toll-like receptor 4, lipopolysaccharide, nuclear factor kappa-B, interleukin (IL)-6 and IL-10 in group F were higher in group F than in group N, whereas the levels of cyclic adenosine monophosphate and aquaporins (AQP) 4 and AQP5 were lower. <i>Proteobacteria</i> were more abundant in group F than in group N, whereas <i>Firmicutes</i>, <i>Bacteroidetes</i>, and <i>Actinobacteria</i> were less abundant.</p><p><strong>Conclusions and relevance: </strong>Oral florfenicol might adversely affect the intestinal mucosa, intestinal mucosal immune system, intestinal microbiota balance, and water metabolism in broilers. This study provides a theoretical basis for the rational use of florfenicol.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 2","pages":"e25"},"PeriodicalIF":1.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11972935/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of formulation and immunogenicity of porcine circovirus type 2d (PCV2d) vaccine for needle-free intradermal route injection.","authors":"Minna Shin, Se-Hui Jung, Kiju Kim, Tae-Wook Hahn","doi":"10.4142/jvs.24323","DOIUrl":"10.4142/jvs.24323","url":null,"abstract":"<p><strong>Importance: </strong>Porcine circovirus type 2 (PCV2) is a major pathogen responsible for the porcine circovirus-associated disease, causing significant economic losses in the swine industry worldwide. PCV2d has become the dominant, following a sequential shift from the previously predominant PCV2a and PCV2b genotypes. Although an effective vaccine based on virus-like particles (VLPs) of recombinant PCV2d-based capsid protein has been developed, intramuscular inoculation of the vaccine still induces a wide variety of side effects. In contrast, intradermal vaccination offers benefits, including enhanced immune activation due to the abundance of dendritic cells in the dermal layer, while also reducing side effects.</p><p><strong>Objective: </strong>Developing a novel vaccine combined with a needle-free inoculation technique is required to elicit protection against PCV2d infection with fewer side effects and higher effectiveness. This study aimed to develop a VLP-based vaccine targeting PCV2d and assess its efficacy when administered intradermally using a needle-free system.</p><p><strong>Methods: </strong>To optimize the intradermal vaccine formulation, we evaluated humoral immunity and neutralizing activity following intradermal administration of test vaccines prepared with varying adjuvant types, adjuvant ratios, and antigen doses.</p><p><strong>Results: </strong>IMS1313 adjuvant provided the best induction of total IgG and neutralizing antibody titers. A dose-dependent evaluation indicated that 20 μg of antigen combined with 40% IMS1313 achieved optimal immune responses. Compared to intramuscular injection, intradermal injection using this formulation induced 1.3-fold higher neutralizing antibody titers, demonstrating higher efficacy.</p><p><strong>Conclusions and relevance: </strong>Intradermal vaccination using a PCV2d VLP-based vaccine improves immunogenicity and cost-effectiveness, providing a promising strategy for controlling PCV2d infections in swine.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 2","pages":"e24"},"PeriodicalIF":1.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11972937/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immortalized porcine mesangial cell line competent for the cultivation of Japanese encephalitis virus.","authors":"Shucheng Zong, Xinze Liu, Wen Pan, Fucheng Guo, Jinyu Fu, Zhongyin Liu, Jin Xiao, Shengkui Xu","doi":"10.4142/jvs.24222","DOIUrl":"10.4142/jvs.24222","url":null,"abstract":"<p><strong>Importance: </strong>The Japanese encephalitis virus (JEV) is a massive threat to the pig-raising industry and attenuated-live vaccines are used widely to prevent JEV infections. Primary hamster kidney cells and Vero cells are mainly used to propagate JEV-attenuated vaccines.</p><p><strong>Objective: </strong>This study aimed to construct porcine mesangial cells (PMCs) for JEV cultivation and study its characteristics.</p><p><strong>Methods: </strong>An immunofluorescence assay was used to analyze the homogeneity of PMCs. Karyotypic analysis and flow cytometry were used to detect the cell chromosome numbers and cell cycle, respectively. TCID₅₀ was used to measure the viral titers.</p><p><strong>Results: </strong>The prepared PMCs were uniform in shape and without variation in chromosome numbers. The PMCs exhibit strong division and proliferation ability, and the JEV could replicate efficiently, having a similar cytopathic effect to that in Vero cells. The viral titer results showed that the JEV grows faster in PMCs than in Vero cells.</p><p><strong>Conclusions and relevance: </strong>This study generated an immortalized PMC cell line suitable for cultivating the JEV, which has significance for producing pig JEV-attenuated vaccines.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 2","pages":"e20"},"PeriodicalIF":1.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11972940/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of five preservation methods for fascia allograft.","authors":"Jiyoung Park, Mu-Young Kim, Hun-Young Yoon","doi":"10.4142/jvs.24276","DOIUrl":"10.4142/jvs.24276","url":null,"abstract":"<p><strong>Importance: </strong>Research on tissue preservation, including cortical bone, skin, nerves, and vessels in glycerol and cortical bone in honey, has shown positive results. On the other hand, relatively few studies have been performed on fascia preservation, and comparisons between different fascia preservation methods remain scarce.</p><p><strong>Objective: </strong>This <i>in vitro</i> study compared the biomechanical properties of five different methods of preserving fascia lata.</p><p><strong>Methods: </strong>The control group underwent biomechanical testing immediately after decellularization, while the other five groups were stored in glycerol, honey, deep freezer, lyophilizer, and liquid nitrogen for 30 days. The ultimate load, elongation at failure, and stiffness for each group were determined from a load-elongation curve.</p><p><strong>Results: </strong>A comparison of the ultimate load showed that the control group had the highest value, followed by the glycerol group. The glycerol group was the only group that did not show a significant difference from the control group, while all the other groups showed a significantly lower ultimate load. A comparison of elongation at failure revealed the glycerol group to have the highest value at failure among all groups and was significantly higher than the deep freeze, honey, and cryopreservation groups.</p><p><strong>Conclusions and relevance: </strong>Glycerol can be used as an effective method for preserving fascia allografts because the resulting allografts show a similar ultimate load to the control group and the highest mean elongation at failure.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 2","pages":"e13"},"PeriodicalIF":1.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11972942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular epidemiology and genetic characteristics of pseudorabies virus between 2021 and 2023 in Henan Province of China.","authors":"Yawei Sun, Mengmeng Shi, Han Yang, Xianfeng Zhang, Yingshuo Zhang, Ruiwu Liu, Leiyin Li, Shuangshuang Li, Xin Zhou, Yongtao Li, Xia Yang, Linyang Yu, Lu Chen","doi":"10.4142/jvs.24243","DOIUrl":"10.4142/jvs.24243","url":null,"abstract":"<p><strong>Importance: </strong>Pseudorabies (PR) is a significant disease that adversely affects the healthy growth of the pig industry in China. However, data on molecular epidemiology and genetic characterization of PRV is sparse.</p><p><strong>Objective: </strong>This study examined the epidemic trends and variation characteristics of the PR virus (PRV) in Henan Province of China.</p><p><strong>Methods: </strong>Six hundred eighty-eight clinically infected pig tissue samples were collected from 18 cities in Henan Province from January 2021 to March 2023 and tested for the PRV. The PRV was isolated from positive samples and identified by a polymerase chain reaction and immunofluorescence assay. The <i>gB</i>, <i>gC</i>, <i>gD</i>, and <i>gE</i> genes of the isolates were then amplified and sequenced for phylogenetic analysis. The <i>gC</i> and <i>gE</i> gene sequences of the isolates in this study were deduced to the respective amino acids for sequence analysis. Selected isolates were evaluated for pathogenicity in KM mice.</p><p><strong>Results: </strong>The positive rate of PRV nucleic acid was 7.99% (55/688) in Henan Province, China. This study isolated eight PRV strains, all of which were genotype II variants. Seven isolates displayed unique amino acid mutations in the gC or gE proteins. The isolates of G22, HN4, QX, and QX2 can cause acute neurological symptoms in KM mice, with 50% lethal dose values of 10^4.2, 10^4.5, 10^5.2, and 10^4.8 50% tissue culture infectious doses, respectively.</p><p><strong>Conclusions and relevance: </strong>This study revealed a high prevalence of the PRV in Henan Province, with genotype II variants being the predominant strains. These findings provide essential data for the prevention and control of the PRV.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 2","pages":"e26"},"PeriodicalIF":1.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11972943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}