Journal of Veterinary Science最新文献

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Radioprotective role of amifostine on osteointegration of titanium implants in the tibia of rats. 氨磷汀对大鼠胫骨钛植入物骨整合的辐射防护作用。
IF 1.8 3区 农林科学
Journal of Veterinary Science Pub Date : 2023-05-01 DOI: 10.4142/jvs.23023
Nevra Aydemir Celep, Hülya Kara, Elif Erbaş, Elif Doğan
{"title":"Radioprotective role of amifostine on osteointegration of titanium implants in the tibia of rats.","authors":"Nevra Aydemir Celep,&nbsp;Hülya Kara,&nbsp;Elif Erbaş,&nbsp;Elif Doğan","doi":"10.4142/jvs.23023","DOIUrl":"https://doi.org/10.4142/jvs.23023","url":null,"abstract":"<p><strong>Background: </strong>Titanium is the most widely used metal for bone integration, especially for cancer patients receiving ionizing radiation. This study aimed to investigate the amifostine administration that would reduce the effects of radiation on bone healing and osseointegration in rat models.</p><p><strong>Objectives: </strong>It is aimed that the application of amifostine in rats receiving radiotherapy treatment will reduce the negative effects of ionizing radiation on the bone.</p><p><strong>Methods: </strong>Thirty-five adult male Wistar rats were randomly divided into one healthy and four experimental groups. In three consecutive days, two experimental groups of rats (AMF-RT-IMP and RT-IMP) were exposed to radiation (15 Gy/3 fractions of 5 Gy each). Then the titanium implants were inserted into the left tibia. Before the radiotherapy process, a 200 mg/kg dose of amifostine (AMF) was administered to the rats in the AMF-IMP and AMF-RT-IMP groups. Twenty-eight days after the screw implant, all rats were sacrificed, and their blood samples and tibia bones were collected for analysis.</p><p><strong>Results: </strong>The results indicated an accelerated bone formation and a more rapid healing process in the screw implants in the AMF-IMP, AMF-RT-IMP, and AMF-RT groups than in the RT-IMP group. Also, bone-implant contact area measurement and inflammation decreased with amifostine treatment in the implants subjected to irradiation (<i>p</i> < 0.05).</p><p><strong>Conclusions: </strong>The results obtained in the present study suggested that amifostine prevents the losses of bone minerals, bone integrity, and implant position from ionizing-radiation when given before exposure.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"24 3","pages":"e35"},"PeriodicalIF":1.8,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b0/3b/jvs-24-e35.PMC10244126.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9651838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Osteochondral allograft transplantation for treating medial femoral condyle subchondral bone cyst in a 14-year-old standardbred horse: a case report. 同种异体骨软骨移植治疗14岁标准种马股骨内侧髁软骨下骨囊肿1例。
IF 1.8 3区 农林科学
Journal of Veterinary Science Pub Date : 2023-05-01 DOI: 10.4142/jvs.22239
Zsófia Pál, Gábor Bodó
{"title":"Osteochondral allograft transplantation for treating medial femoral condyle subchondral bone cyst in a 14-year-old standardbred horse: a case report.","authors":"Zsófia Pál,&nbsp;Gábor Bodó","doi":"10.4142/jvs.22239","DOIUrl":"https://doi.org/10.4142/jvs.22239","url":null,"abstract":"<p><p>Allograft arthroplasty is a promising cartilage-resurfacing technique. A 14-year-old horse was diagnosed with a medial femoral condyle subchondral bone cyst. Allografts were harvested from a young donor animal and implanted to fill the cyst cavity. A visual assessment of the surgical site was possible during follow-up arthroscopy. In addition to a desirable gliding surface and a good adaptation of the grafts, fibrillation of the cranial ligament of the medial meniscus was noted and debrided. The recipient horse became sound four months after follow-up surgery with a high level of owner satisfaction. In conclusion allograft transplantation is a promising technique for treating subchondral bone cysts.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"24 3","pages":"e31"},"PeriodicalIF":1.8,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ad/69/jvs-24-e31.PMC10244131.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9651836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lumpy skin disease as an emerging infectious disease. 结节性皮肤病是一种新兴的传染病。
IF 1.8 3区 农林科学
Journal of Veterinary Science Pub Date : 2023-05-01 DOI: 10.4142/jvs.23016
Hye Jin Eom, Eun-Seo Lee, Han Sang Yoo
{"title":"Lumpy skin disease as an emerging infectious disease.","authors":"Hye Jin Eom,&nbsp;Eun-Seo Lee,&nbsp;Han Sang Yoo","doi":"10.4142/jvs.23016","DOIUrl":"https://doi.org/10.4142/jvs.23016","url":null,"abstract":"<p><p>Lumpy skin disease (LSD) is one of the most important emerging transboundary diseases. Recently, LSD has emerged in many countries in the northern hemisphere. The LSD virus has a huge genome and is highly resistant to environmental conditions. The virus is also host-specific and large ruminants, such as cattle and domestic water buffalo, are particularly susceptible. In addition, wild ruminants can serve as potential reservoirs for spreading the LSD virus. The emergence might be related to climate change in various regions because LSD is an arthropod-borne infectious disease. This disease causes enormous economic losses, such as leather damage, decreased milk production, abortion, and death in infected ruminants. The economic importance of LSD in the bovine industry has forced countries to develop and implement control strategies against the disease. With the recent global spread and the economic impact, LSD will be discussed intensively. In addition, effective preventive measures are suggested based on the presence or absence of LSD outbreaks.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"24 3","pages":"e42"},"PeriodicalIF":1.8,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b7/0c/jvs-24-e42.PMC10244130.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9651843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Novel pan-lineage VP1 specific degenerate primers for precise genetic characterization of serotype O foot and mouth disease virus circulating in India. 新型泛谱系VP1特异性退化引物用于印度流行的血清O型口蹄疫病毒的精确遗传表征
IF 1.8 3区 农林科学
Journal of Veterinary Science Pub Date : 2023-05-01 DOI: 10.4142/jvs.22292
Sagar Ashok Khulape, Jitendra Kumar Biswal, Chandrakanta Jana, Saravanan Subramaniam, Rabindra Prasad Singh
{"title":"Novel pan-lineage VP1 specific degenerate primers for precise genetic characterization of serotype O foot and mouth disease virus circulating in India.","authors":"Sagar Ashok Khulape,&nbsp;Jitendra Kumar Biswal,&nbsp;Chandrakanta Jana,&nbsp;Saravanan Subramaniam,&nbsp;Rabindra Prasad Singh","doi":"10.4142/jvs.22292","DOIUrl":"https://doi.org/10.4142/jvs.22292","url":null,"abstract":"<p><p>Analysis of the VP1 gene sequence of the foot and mouth disease virus (FMDV) is critical to understanding viral evolution and disease epidemiology. A standard set of primers have been used for the detection and sequence analysis of the VP1 gene of FMDV directly from suspected clinical samples with limited success. The study validated VP1-specific degenerate primer-based reverse transcription polymerase chain reaction (RT-PCR) for the qualitative detection and sequencing of serotype O FMDV lineages circulating in India. The novel degenerate primer-based RT-PCR amplifying the VP1 gene can circumvent the genetic heterogeneity observed in viruses after cell culture adaptation and facilitate precise viral gene sequence analysis from clinical samples.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"24 3","pages":"e40"},"PeriodicalIF":1.8,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3f/69/jvs-24-e40.PMC10244134.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9651840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ectopic insulinoma in a dog with insulin-induced hypoglycemia: a case report. 犬异位胰岛素瘤伴胰岛素性低血糖:1例报告。
IF 1.8 3区 农林科学
Journal of Veterinary Science Pub Date : 2023-05-01 DOI: 10.4142/jvs.22328
Jiwon Kim, Insun Hwang, Danbee Kwon, Kanghyo Park, Hakyoung Yoon
{"title":"Ectopic insulinoma in a dog with insulin-induced hypoglycemia: a case report.","authors":"Jiwon Kim,&nbsp;Insun Hwang,&nbsp;Danbee Kwon,&nbsp;Kanghyo Park,&nbsp;Hakyoung Yoon","doi":"10.4142/jvs.22328","DOIUrl":"https://doi.org/10.4142/jvs.22328","url":null,"abstract":"<p><p>A 7-year-old spayed female Shih Tzu dog was presented for evaluation of recurrent hypoglycemia. Serum insulin levels during hypoglycemia were 35.3 μIU/mL. Ultrasonography and computed tomography showed a mesenteric nodule between the kidney and the portal vein, but no pancreatic mass was observed. During surgery, the nodule had neither anatomical adhesions nor vascular connections to the pancreas. Pancreatic inspection and palpation revealed no abnormalities. Hypoglycemia improved after resection of the nodule. Histopathological examination confirmed the nodule to be an islet cell carcinoma. Although extremely rare, ectopic insulinoma should be considered as a possible cause of insulin-induced hypoglycemia in dogs.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"24 3","pages":"e39"},"PeriodicalIF":1.8,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/65/23/jvs-24-e39.PMC10244139.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9651842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Application of tenotomy on Korean native cattle (Hanwoo) with spastic paresis symptoms in the field. 肌腱切断术在有痉挛性轻瘫症状的韩牛中的应用。
IF 1.8 3区 农林科学
Journal of Veterinary Science Pub Date : 2023-05-01 DOI: 10.4142/jvs.23018
Younghye Ro, Woojae Choi, Leegon Hong, Kyunghyun Min, Inkwang Ryu, Danil Kim
{"title":"Application of tenotomy on Korean native cattle (Hanwoo) with spastic paresis symptoms in the field.","authors":"Younghye Ro,&nbsp;Woojae Choi,&nbsp;Leegon Hong,&nbsp;Kyunghyun Min,&nbsp;Inkwang Ryu,&nbsp;Danil Kim","doi":"10.4142/jvs.23018","DOIUrl":"https://doi.org/10.4142/jvs.23018","url":null,"abstract":"<p><p>Bovine spastic paresis (BSP) is a neuromuscular disorder characterized by hypertension and stiffness of hindlimb. Two Korean native cattle (Hanwoo) calves developed BSP or BSP-like symptoms, and a tenotomy of superficial tendon of medial head and deep tendon of lateral head of gastrocnemius muscle was performed for treatment. A cast was applied postoperatively to prevent muscle rupture and was removed three weeks later. The prognosis was evaluated at 3 weeks, 6 and 18 months postoperatively. Neither calf showed any other postoperative sequelae. This is the first case study to report the diagnosis, treatment, and prognosis of BSP in Hanwoo.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"24 3","pages":"e45"},"PeriodicalIF":1.8,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0d/bc/jvs-24-e45.PMC10244129.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9596257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Therapeutic effects of paeoniflorin on irritable bowel syndrome in rats. 芍药苷对大鼠肠易激综合征的治疗作用
IF 1.8 3区 农林科学
Journal of Veterinary Science Pub Date : 2023-05-01 DOI: 10.4142/jvs.22083
Lei Wang, Jinyan Lei, Zeyu Zhao, Jianwei Jia, Li Wang
{"title":"Therapeutic effects of paeoniflorin on irritable bowel syndrome in rats.","authors":"Lei Wang, Jinyan Lei, Zeyu Zhao, Jianwei Jia, Li Wang","doi":"10.4142/jvs.22083","DOIUrl":"10.4142/jvs.22083","url":null,"abstract":"<p><strong>Background: </strong>Irritable bowel syndrome (IBS) is a functional bowel disorder (FBD).</p><p><strong>Objectives: </strong>To assess the therapeutic effects of paeoniflorin (PF) on IBS in rats.</p><p><strong>Method: </strong>Sixty male Sprague-Dawley rats were randomly divided into normal, model, positive drug, low-dose PF, medium-dose PF and high-dose PF groups (n = 10). After gavage for 2 consecutive weeks, the effect of PF on abdominal pain symptoms was assessed based on the abdominal withdrawal reflex (AWR) score, fecal water content and pathological changes in colon tissues. D-lactate, interleukin-1β (IL-1β), transforming growth factor-β (TGF-β) and tumor necrosis factor-α (TNF-α) were detected by enzyme-linked immunosorbent assay, and phosphorylated nuclear factor kappa B (p-NF-κB) p65 was detected by Western blotting. The abundance and diversity changes of intestinal flora were explored using 16S ribosomal RNA sequencing.</p><p><strong>Result: </strong>In PF groups, the mucosal morphology of colon tissues was intact, and the glands were arranged neatly and structured clearly, without obvious inflammatory cell infiltration. Compared with the model group, PF groups had significantly elevated pain threshold, and mRNA and protein levels of zonula occludens-1 (ZO-1) and occludin, decreased AWR score at 20 mmHg pressure, fecal water content, mRNA levels of IL-1β, TGF-β, and TNF-α, protein level of p-NF-κB p65 and level of serum D-lactate, and reduced levels of serum IL-1β, TGF-β, and TNF-α (<i>p</i> < 0.05, <i>p</i> < 0.01). PF groups had higher abundance of <i>Lactobacillus</i>, <i>Akkermansia</i>, <i>Alistipes</i>, and <i>Bacteroides</i>, but lower abundance of <i>Desulfovibrio</i>, <i>Parasutterella</i>, and <i>Enterococcus</i> than those of the model group.</p><p><strong>Conclusions: </strong>PF exerts therapeutic effects on IBS in rats probably by regulating the intestinal flora, and then up-regulating the expressions of ZO-1 and occludin in colon tissue while down-regulating the levels of IL-1β, TGF-β, TNF-α, D-lactate and p-NF-κB p65.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"24 3","pages":"e23"},"PeriodicalIF":1.8,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f2/0c/jvs-24-e23.PMC10244138.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9651837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Establishing veterinary graduation competencies and its impact on veterinary medical education in Korea. 建立兽医毕业能力及其对韩国兽医教育的影响。
IF 1.8 3区 农林科学
Journal of Veterinary Science Pub Date : 2023-05-01 DOI: 10.4142/jvs.22258
Sang-Soep Nahm, Kichang Lee, Myung Sun Chun, Jongil Kang, Seungjoon Kim, Seong Mok Jeong, Jin Young Chung, Pan Dong Ryu
{"title":"Establishing veterinary graduation competencies and its impact on veterinary medical education in Korea.","authors":"Sang-Soep Nahm,&nbsp;Kichang Lee,&nbsp;Myung Sun Chun,&nbsp;Jongil Kang,&nbsp;Seungjoon Kim,&nbsp;Seong Mok Jeong,&nbsp;Jin Young Chung,&nbsp;Pan Dong Ryu","doi":"10.4142/jvs.22258","DOIUrl":"https://doi.org/10.4142/jvs.22258","url":null,"abstract":"<p><p>Competencies are defined as an observable and assessable set of knowledge, skills, and attitudes. Graduation competencies, which are more comprehensive, refer to the required abilities of students to perform on-site work immediately after graduation. As graduation competencies set the goal of education, various countries and institutions have introduced them for new veterinary graduates. The Korean Association of Veterinary Medical Colleges has recently established such competencies to standardize veterinary education and enhance quality levels thereof. The purpose of this study is to describe the process of establishing graduation competencies as well as their implication for veterinary education in Korea. Graduation competencies for veterinary education in Korea comprise 5 domains (animal health care and disease management, one health expertise, communication and collaboration, research and learning, and veterinary professionalism). These are further divided into 11 core competencies, and 33 achievement standards, which were carefully chosen from previous case analyses and nation-wide surveys. Currently, graduation competencies are used as a standard for setting clear educational purposes for both instructors and students. Establishing these competencies further initiated the development of detailed learning outcomes, and of a list of basic veterinary clinical performances and skills, which is useful for assessing knowledge and skills. The establishment of graduation competencies is expected to contribute to the continuous development of Korean veterinary education in many ways. These include curriculum standardization and licensing examination reform, which will eventually improve the competencies of new veterinary graduates.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"24 3","pages":"e41"},"PeriodicalIF":1.8,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/76/0b/jvs-24-e41.PMC10244128.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9651841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Prevalence of Senecavirus A in pigs from 2014 to 2020: a global systematic review and meta-analysis. 2014年至2020年猪中塞尼加病毒A的流行:一项全球系统综述和荟萃分析
IF 1.8 3区 农林科学
Journal of Veterinary Science Pub Date : 2023-05-01 DOI: 10.4142/jvs.22307
Xuhua Ran, Zhenru Hu, Jun Wang, Zhiyuan Yang, Zhongle Li, Xiaobo Wen
{"title":"Prevalence of Senecavirus A in pigs from 2014 to 2020: a global systematic review and meta-analysis.","authors":"Xuhua Ran,&nbsp;Zhenru Hu,&nbsp;Jun Wang,&nbsp;Zhiyuan Yang,&nbsp;Zhongle Li,&nbsp;Xiaobo Wen","doi":"10.4142/jvs.22307","DOIUrl":"https://doi.org/10.4142/jvs.22307","url":null,"abstract":"<p><strong>Background: </strong>Senecavirus A (SVA), a member of the family <i>Picornaviridae</i>, is newly discovered, which causes vesicular lesions, lameness in swine, and even death in neonatal piglets. SVA has rapidly spread worldwide in recent years, especially in Asia.</p><p><strong>Objectives: </strong>We conducted a global meta-analysis and systematic review to determine the status of SVA infection in pigs.</p><p><strong>Methods: </strong>Through PubMed, VIP Chinese Journals Database, China National Knowledge Infrastructure, and Wanfang Data search data from 2014 to July 26, 2020, a total of 34 articles were included in this analysis based on our inclusion criteria. We estimated the pooled prevalence of SVA in pigs by the random effects model. A risk of bias assessment of the studies and subgroup analysis to explain heterogeneity was undertaken.</p><p><strong>Results: </strong>We estimated the SVA prevalence to be 15.90% (1,564/9,839; 95% confidence interval [CI], 44.75-65.89) globally. The prevalence decreased to 11.06% (945/8,542; 95% CI, 28.25-50.64) after 2016. The highest SVA prevalence with the VP1-based RT-PCR and immunohistochemistry assay was 58.52% (594/1,015; 95% CI, 59.90-83.96) and 85.54% (71/83; 95% CI, 76.68-100.00), respectively. Besides, the SVA prevalence in piglet herds was the highest at 71.69% (119/166; 95% CI, 68.61-98.43) (<i>p</i> < 0.05). Moreover, our analysis confirmed that the subgroups, including country, sampling year, sampling position, detected gene, detection method, season, age, and climate, could be the heterogeneous factors associated with SVA prevalence.</p><p><strong>Conclusions: </strong>The results indicated that SVA widely exists in various countries currently. Therefore, more prevention and control policies should be proposed to enhance the management of pig farms and improve breeding conditions and the environment to reduce the spread of SVA.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"24 3","pages":"e48"},"PeriodicalIF":1.8,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9e/b1/jvs-24-e48.PMC10244137.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9599486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In vitro effects of monophosphoryl lipid A and Poly I:C combination on equine cells. 单磷酰脂A和聚I:C联合对马细胞的体外影响。
IF 1.8 3区 农林科学
Journal of Veterinary Science Pub Date : 2023-05-01 DOI: 10.4142/jvs.23007
Dong-Ha Lee, Eun-Bee Lee, Jong-Pil Seo, Eun-Ju Ko
{"title":"<i>In vitro</i> effects of monophosphoryl lipid A and Poly I:C combination on equine cells.","authors":"Dong-Ha Lee,&nbsp;Eun-Bee Lee,&nbsp;Jong-Pil Seo,&nbsp;Eun-Ju Ko","doi":"10.4142/jvs.23007","DOIUrl":"https://doi.org/10.4142/jvs.23007","url":null,"abstract":"<p><strong>Background: </strong>Toll-like receptor (TLR) agonists have been used as adjuvants to modulate immune responses in both animals and humans.</p><p><strong>Objectives: </strong>The objective of this study was to evaluate the combined effects of the TLR 4 agonist monophosphoryl lipid A (MPL) and the TLR 3 agonist polyinosinic:polycytidylic acid (Poly I:C) on equine peripheral blood mononuclear cells (PBMCs), monocyte-derived dendritic cells (MoDCs), and bone marrow-derived mesenchymal stromal cells (BM-MSCs).</p><p><strong>Methods: </strong>The PBMCs, MoDCs, and BM-MSCs collected from three mixed breed horses were treated with MPL, Poly I:C, and their combination. The mRNA expression of interferon gamma (IFN-γ), interleukin (IL)-1β, IL-4, IL-6, IL-8, IL-12p40, tumor necrosis factor alpha (TNF-α), vascular endothelial growth factor (VEGF), and monocyte chemoattractant protein-1 (MCP-1) was determined using real-time polymerase chain reaction.</p><p><strong>Results: </strong>The combination of MPL and Poly I:C significantly upregulated immunomodulatory responses in equine cells/ without cytotoxicity. The combination induced greater mRNA expression of pro-inflammatory cytokines IFN-γ and IL-6 than MPL or Poly I:C stimulation alone in PBMCs. In addition, the combination induced significantly higher mRNA expression of IL-1β, IL-6, and IL-12p40 in MoDCs, and IL-8, MCP-1, and VEGF in BM-MSCs compared to stimulation with a single TLR agonist.</p><p><strong>Conclusions: </strong>The combination of MPL and Poly I:C can be used as a potential adjuvant candidate for vaccines to aid in preventing infectious diseases in horses.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"24 3","pages":"e37"},"PeriodicalIF":1.8,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/40/28/jvs-24-e37.PMC10244135.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9599487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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