Journal of Veterinary Science最新文献_第10页

Elution profiles of metronidazole from calcium sulfate beads. 甲硝唑从硫酸钙珠中的洗脱特性。

IF 1.8 3区农林科学

Journal of Veterinary Science Pub Date : 2023-11-01 Epub Date: 2023-09-18 DOI: 10.4142/jvs.23166

Burasarin Ithisariyanont, Saranya Poapolathep, Amnart Poapolathep, Pareeya Udomkusonsri

{"title":"Elution profiles of metronidazole from calcium sulfate beads.","authors":"Burasarin Ithisariyanont, Saranya Poapolathep, Amnart Poapolathep, Pareeya Udomkusonsri","doi":"10.4142/jvs.23166","DOIUrl":"10.4142/jvs.23166","url":null,"abstract":"Background Antibiotic beads are used to treat local bacterial infections by delivering high drug concentrations to infected tissue. Objectives This study examined the elution characteristics of metronidazole from metronidazole-calcium sulfate (MCa) and metronidazole-calcium-potassium sulfate (MCaK) beads over 20 days and the antibacterial efficacy of the beads after storage. Methods The MCa and MCaK beads were prepared by mixing 250 mg of metronidazole and 10 g of calcium sulfate hemihydrate with water and a 3% potassium sulfate solution, respectively. The beads were placed in phosphate-buffered saline for the elution study. The metronidazole eluents were determined using high-performance liquid chromatography. The microstructures were examined by scanning electron microscopy (SEM), and the antimicrobial activity was evaluated by a microbioassay. Results For the 20-day study, the total amount of metronidazole released was greater in the MCa beads than in the MCaK beads by 6.61 ± 0.48 mg (89.11% ± 3.04%) and 4.65 ± 0.36 mg (73.11% ± 4.38%), respectively. The amounts of eluted drugs from the MCa and MCaK beads were higher than the minimum inhibitory concentration at 0.5 µg/mL against anaerobic bacteria at both 20 days and 14 days. SEM showed that calcium crystals on the outer surface had dissolved after elution, and thinner calcium crystals were prominent in the MCaK beads. The MCa and MCaK beads exhibited antibacterial activity after setting, followed by storage at room temperature or 4°C for 21 days. Conclusions The MCa beads could release more drug than the MCaK beads, but all eluted metronidazole amounts were effective in controlling bacterial infections. Both metronidazole beads could be stored at ambient temperature or in a refrigerator.","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":" ","pages":"e74"},"PeriodicalIF":1.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10694377/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71412733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of caspase-1-dependent apoptosis suppresses peste des petits ruminants virus replication. 胱天蛋白酶-1依赖性细胞凋亡的抑制抑制小反刍动物病毒的复制。

IF 1.8 3区农林科学

Journal of Veterinary Science Pub Date : 2023-09-01 Epub Date: 2023-07-07 DOI: 10.4142/jvs.22288

Lingxia Li, Shengqing Li, Shengyi Han, Pengfei Li, Guoyu Du, Jinyan Wu, Xiaoan Cao, Youjun Shang

{"title":"Inhibition of caspase-1-dependent apoptosis suppresses peste des petits ruminants virus replication.","authors":"Lingxia Li, Shengqing Li, Shengyi Han, Pengfei Li, Guoyu Du, Jinyan Wu, Xiaoan Cao, Youjun Shang","doi":"10.4142/jvs.22288","DOIUrl":"10.4142/jvs.22288","url":null,"abstract":"Background: Peste des petits ruminants (PPR), caused by the PPR virus (PPRV), is an acute and fatal contagious disease that mainly infects goats, sheep, and other artiodactyls. Peripheral blood mononuclear cells (PBMCs) are considered the primary innate immune cells.Objectives: PBMCs derived from goats were infected with PPRV and analyzed to detect the relationship between PPRV replication and apoptosis or the inflammatory response.Methods: Quantitative real-time polymerase chain reaction was used to identify PPRV replication and cytokines expression. Flow cytometry was conducted to detect apoptosis and the differentiation of CD4+ and CD8+ T cells after PPRV infection.Results: PPRV stimulated the differentiation of CD4+ and CD8+ T cells. In addition, PPRV induced apoptosis in goat PBMCs. Furthermore, apoptosis and the inflammatory response induced by PPRV could be suppressed by Z-VAD-FMK and Z-YVAD-FMK, respectively. Moreover, the virus titer of PPRV was attenuated by inhibiting caspase-1-dependent apoptosis and inflammation.Conclusions: This study showed that apoptosis and the inflammatory response play an essential role in PPR viral replication in vitro, providing a new mechanism related to the cell host response.","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":" ","pages":"e55"},"PeriodicalIF":1.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6d/f0/jvs-24-e55.PMC10556287.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10085897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protection provided by a commercial modified-live porcine reproductive and respiratory syndrome virus (PRRSV) 1 vaccine (PRRSV1-MLV) against a Japanese PRRSV2 field strain. 由商业改良的猪繁殖和呼吸综合征活病毒（PRRSV）1疫苗（PRRSV1-MLV）对日本PRRSV2现场株提供的保护。

IF 1.8 3区农林科学

Journal of Veterinary Science Pub Date : 2023-09-01 Epub Date: 2023-07-07 DOI: 10.4142/jvs.23025

Joel Miranda, Salvador Romero, Lidia de Lucas, Fumitoshi Saito, Mar Fenech, Ivan Díaz

{"title":"Protection provided by a commercial modified-live porcine reproductive and respiratory syndrome virus (PRRSV) 1 vaccine (PRRSV1-MLV) against a Japanese PRRSV2 field strain.","authors":"Joel Miranda, Salvador Romero, Lidia de Lucas, Fumitoshi Saito, Mar Fenech, Ivan Díaz","doi":"10.4142/jvs.23025","DOIUrl":"10.4142/jvs.23025","url":null,"abstract":"Background: Porcine reproductive and respiratory syndrome virus (PRRSV) vaccines do not provide full cross-protection, mainly due to the virus genetic variability. Despite this, vaccines based on modified-live PRRSV (PRRSV-MLV) reduce the disease impact.Objectives: To assess the efficacy of two commercial vaccines-one based on PRRSV1 (PRRSV1-MLV) and another on PRRSV2 (PRRSV2-MLV)-against a Japanese PRRSV2 field strain.Methods: Two groups of three-week-old piglets were vaccinated (G1: PRRSV1-MLV; G2: PRRSV2-MLV) and two were kept as non-vaccinated (INF and CTRL). One month later, G1, G2, and INF were challenged with a PRRSV2 field strain.Results: After the challenge, clinical signs were only observed in INF. Moreover, the highest rectal temperatures and values for the area under the curve (AUC) were observed in INF. Regarding viral detection, both AUC and the proportion of positive samples in blood were higher in INF. In G1, viremic animals never reached 100%. At necropsy (21 d after the challenge), differences for titers among groups were only found in tonsils (G1 < G2 and INF). One animal (belonging to G1) was negative in all tissues. Regarding humoral responses, G1 and G2 seroconverted after vaccination, as detected in the corresponding enzyme-linked immunosorbent assay. Specific neutralizing antibodies (NA) against PRRSV1-MLV were already detected at 14 d after vaccination in G1, showing a significant booster after the challenge, while PRRSV2-MLV NA were detected in G2 at the end of the experiment.Conclusions: Despite genetic differences, PRRSV1-MLV has been demonstrated to confer partial protection against a Japanese PRRSV2 strain, at least as good as PRRSV2-MLV.","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":" ","pages":"e54"},"PeriodicalIF":1.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6c/af/jvs-24-e54.PMC10556292.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10085900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nutritional evaluation of new alternative types of dog foods including raw and cooked homemade-style diets. 新的替代类型狗粮的营养评估，包括生的和煮熟的自制风格的饮食。

IF 1.8 3区农林科学

Journal of Veterinary Science Pub Date : 2023-09-01 Epub Date: 2023-08-07 DOI: 10.4142/jvs.23037

Boyeon Choi, San Kim, Goo Jang

{"title":"Nutritional evaluation of new alternative types of dog foods including raw and cooked homemade-style diets.","authors":"Boyeon Choi, San Kim, Goo Jang","doi":"10.4142/jvs.23037","DOIUrl":"10.4142/jvs.23037","url":null,"abstract":"Background: New alternative types of pet foods such as raw and cooked homemade-style diets containing human food ingredients have been introduced due to a trend of pet humanization and diversification of consumer needs.Objectives: To evaluate nutritional adequacy of new alternative types of dog foods containing human food ingredients as maintenance diets for dogs.Methods: Eleven homemade-style foods for adult dogs were purchased from online channel in Korea and analyzed to evaluate nutritional adequacy for adult dogs. Nutrients analyzed included crude protein, amino acids, crude fat, fatty acids, and minerals.Results: Crude protein and amino acids in all products satisfied Association of American Feed Control Officials (AAFCO) requirements. Crude fat in one of 11 products did not meet AAFCO requirements. The most deficient minerals were selenium (10 of 11, 90.9%), copper (five of 11, 45.5%), zinc (five of 11, 45.5%), potassium (three of 11, 27.3%), calcium (three of 11, 27.3%), iron (two of 11, 18.2%), and magnesium (one of 11, 9.1%). Six products were not in the range of the recommended Ca:P ratio in AAFCO dog food maintenance nutrient profiles.Conclusions: This study performed nutritional evaluation of raw and cooked homemade-style foods as maintenance diets for adult dogs. Some nutritional inadequacies were observed including some minerals, Ca:P ratio, and omega-6:omega-3 fatty acid ratio, although three products (26.2%) satisfied the AAFCO standard except selenium. Overall, the data suggest a need for accurate nutritional adequacy statement for consumers based on proper methods to validate the formula.","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":" ","pages":"e63"},"PeriodicalIF":1.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/cf/f7/jvs-24-e63.PMC10556285.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10085902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metastatic intestinal adenocarcinoma with osseous metaplasia in two Domestic Korean Shorthair cats. 两只韩国短毛猫的转移性肠腺癌伴骨化生。

IF 1.8 3区农林科学

Journal of Veterinary Science Pub Date : 2023-09-01 Epub Date: 2023-08-08 DOI: 10.4142/jvs.23124

Jae-Ha Jung, Na-Yon Kim, Yeseul Yang, Dansong Seo, Goeun Choi, Hyunki Hong, Taeseong Moon, Hyeong-Mok Kim, Jihee Han, Jihee Hong, Yongbaek Kim

引用次数: 0

Review of outcomes of using lower ethanol concentration (83%) in percutaneous ultrasound-guided renal cyst sclerotherapy in dogs. 低乙醇浓度（83%）经皮超声引导下犬肾囊肿硬化治疗的疗效评价。

IF 1.8 3区农林科学

Journal of Veterinary Science Pub Date : 2023-09-01 Epub Date: 2023-08-07 DOI: 10.4142/jvs.23045

Sanghyeon Yoon, Jungmin Kwak, Deokho Im, Hakyoung Yoon

{"title":"Review of outcomes of using lower ethanol concentration (83%) in percutaneous ultrasound-guided renal cyst sclerotherapy in dogs.","authors":"Sanghyeon Yoon, Jungmin Kwak, Deokho Im, Hakyoung Yoon","doi":"10.4142/jvs.23045","DOIUrl":"10.4142/jvs.23045","url":null,"abstract":"Background: Percutaneous renal cyst sclerotherapy (PRCS) as a treatment for renal cysts is usually performed with a high concentration of ethanol (≥ 90%). This study reviewed cases in which a lower concentration of ethanol (83%) was used for the procedure in dogs.Methods: Records of cases of renal cysts treated by sclerotherapy using 83% ethanol in dogs were reviewed. Outcomes of the treatment were evaluated by comparing volumes of renal cysts before the procedure and the volumes after treatment, using ultrasound images with the volume reduction rates classified as follows: < 50% of initial volume (failed); ≥ 50% but < 80% of initial volume (partial success); ≥ 80% but < 95% of initial volume (great success); ≥ 95% of initial volume (complete success).Results: Out of nine dog kidneys, renal cysts sclerotherapy with 83% ethanol achieved partial success in one kidney, great success in four, and complete success in the other four. No side effect was observed. The mean of the volume-reduction rates was 90.00 ± 11.00 while the minimum and maximum reduction rates were 65% and 100%, respectively.Conclusions: The lower ethanol concentration (83%) is good for disinfecting kidneys in PRCS.","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":" ","pages":"e61"},"PeriodicalIF":1.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f1/79/jvs-24-e61.PMC10556289.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10085898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hematological differentiation of bladder rupture and complete/partial urethral obstruction in castrated Hanwoo (Korean indigenous cattle) with urolithiasis. 患有尿石症的阉割韩屋（韩国土著牛）膀胱破裂和完全/部分尿道梗阻的血液学鉴别。

IF 1.8 3区农林科学

Journal of Veterinary Science Pub Date : 2023-09-01 Epub Date: 2023-08-07 DOI: 10.4142/jvs.23010

Young-Jun Kim, Seung-Min Ha, Ji-Yeong Ku, Ji-Seon Yoon, Jinho Park

引用次数: 0

Cross-sectional and skeletal anatomy of long-tailed gorals (Naemorhedus caudatus) using imaging evaluations. 使用成像评估的长尾鼬横切面和骨骼解剖。

IF 1.8 3区农林科学

Journal of Veterinary Science Pub Date : 2023-07-01 DOI: 10.4142/jvs.23076

Sangjin Ahn, Woojin Shin, Yujin Han, Sohwon Bae, Cheaun Cho, Sooyoung Choi, Jong-Taek Kim

{"title":"Cross-sectional and skeletal anatomy of long-tailed gorals (Naemorhedus caudatus) using imaging evaluations.","authors":"Sangjin Ahn, Woojin Shin, Yujin Han, Sohwon Bae, Cheaun Cho, Sooyoung Choi, Jong-Taek Kim","doi":"10.4142/jvs.23076","DOIUrl":"https://doi.org/10.4142/jvs.23076","url":null,"abstract":"Background: Accurate diagnosis of diseases in animals is crucial for their treatment, and imaging evaluations such as radiographs, computed tomography (CT), and magnetic resonance imaging (MRI) are important tools for this purpose. However, a cross-sectional anatomical atlas of normal skeletal and internal organs of long-tailed gorals (Naemorhedus caudatus) has not yet been prepared for diagnosing their diseases.Objectives: The objective of this study was to create an anatomical atlas of gorals using CT and MRI, which are imaging techniques that have not been extensively studied in this type of wild animal in Korea.Methods: The researchers used CT and MRI to create an anatomical atlas of gorals, and selected 37 cross-sections from the head, thoracic, lumbar, and sacrum parts of gorals to produce an average cross-sectional anatomy atlas.Results: This study successfully created an anatomical atlas of gorals using CT and MRI.Conclusions: The atlas provides valuable information for the diagnosis of diseases in gorals, which can improve their treatment and welfare. The study highlights the importance of developing cross-sectional anatomical atlases of gorals to diagnose and treat their diseases effectively.","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"24 4","pages":"e60"},"PeriodicalIF":1.8,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6b/a1/jvs-24-e60.PMC10404708.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9950024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of cold atmospheric microwave plasma as an adjunct therapy for wound healing in dogs and cats. 低温大气微波等离子体辅助治疗犬、猫伤口愈合的应用。

IF 1.8 3区农林科学

Journal of Veterinary Science Pub Date : 2023-07-01 DOI: 10.4142/jvs.23067

Jisu Yoo, Yeong-Hun Kang, Seung Joon Baek, Cheol-Yong Hwang

{"title":"Application of cold atmospheric microwave plasma as an adjunct therapy for wound healing in dogs and cats.","authors":"Jisu Yoo, Yeong-Hun Kang, Seung Joon Baek, Cheol-Yong Hwang","doi":"10.4142/jvs.23067","DOIUrl":"https://doi.org/10.4142/jvs.23067","url":null,"abstract":"Background: Cold atmospheric plasma is a novel innovative approach for wound care, and it is currently underrepresented in veterinary medicine.Objectives: To investigate the efficacy and safety of using cold atmospheric microwave plasma (CAMP) as an adjunct therapy for wound healing in dogs and cats.Methods: Wound healing outcomes were retrospectively analyzed using clinical records of client-owned dogs and cats who were first managed through standard wound care alone (pre-CAMP period) and subsequently via CAMP therapy (CAMP period). The degree of wound healing was estimated based on wound size and a modified wound scoring system.Results: Of the 27 acute and chronic wounds included in the analysis, 81.48% showed complete healing after the administration of CAMP as an adjunct therapy to standard care. Most wounds achieved complete healing in < 5 weeks. Compared with the pre-CAMP period, the rate of wound healing significantly increased every week in the CAMP period in terms of in wound size (first week, p < 0.001; second week, p = 0.012; third week, p < 0.001) and wound score (first week, p < 0.001; second week, p < 0.001; third week, p = 0.001). No adverse events were noted except for mild discomfort and transient erythema.Conclusions: CAMP is a well-tolerated therapeutic option with immense potential to support the treatment of wounds of diverse etiology in small animal practice. Further research is warranted to establish specific criteria for CAMP treatment according to wound characteristics.","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"24 4","pages":"e56"},"PeriodicalIF":1.8,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/64/be/jvs-24-e56.PMC10404707.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9956290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vascular ring anomaly with a right patent ductus arteriosus and a left aortic arch in a juvenile cat. 幼猫血管环异常伴右侧动脉导管未闭和左侧主动脉弓。

IF 1.8 3区农林科学

Journal of Veterinary Science Pub Date : 2023-07-01 DOI: 10.4142/jvs.22290

Aki Takeuchi, Kazumi Shimada, Lina Hamabe, Tomohiko Yoshida, Yusuke Ozai, Miki Hirose, Aimi Yokoi, Momoko Watanabe, Ikki Mitsui, Ryou Tanaka

引用次数: 0