Effect of Silencing subolesin and enolase impairs gene expression, engorgement and reproduction in Haemaphysalis longicornis (Acari: Ixodidae) ticks.

IF 1.5 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Veterinary Science Pub Date : 2024-05-01 DOI:10.4142/jvs.24039

Md Samiul Haque, Mohammad Saiful Islam, Myung-Jo You

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引用次数: 0

Abstract

Importance: Haemaphysalis longicornis is an obligate blood-sucking ectoparasite that has gained attention due its role of transmitting medically and veterinary significant pathogens and it is the most common tick species in Republic of Korea. The preferred strategy for controlling ticks is a multi-antigenic vaccination. Testing the efficiency of a combination antigen is a promising method for creating a tick vaccine.

Objective: The aim of the current research was to analyze the role of subolesin and enolase in feeding and reproduction of H. longicornis by gene silencing.

Methods: In this study, we used RNA interference to silence salivary enolase and subolesin in H. longicornis. Unfed female ticks injected with double-stranded RNA targeting subolesin and enolase were attached and fed normally on the rabbit's ear. Real-time polymerase chain reaction was used to confirm the extent of knockdown.

Results: Ticks in the subolesin or enolase dsRNA groups showed knockdown rates of 80% and 60% respectively. Ticks in the combination dsRNA (subolesin and enolase) group showed an 80% knockdown. Knockdown of subolesin and enolase resulted in significant depletion in feeding, blood engorgement weight, attachment rate, and egg laying. Silencing of both resulted in a significant (p < 0.05) reduction in tick engorgement, egg laying, egg hatching (15%), and reproduction.

Conclusions and relevance: Our results suggest that subolesin and enolase are an exciting target for future tick control strategies.

查看原文本刊更多论文

沉默亚叶绿素和烯醇化酶的影响会损害 Haemaphysalis longicornis (Acari: Ixodidae) 蜱的基因表达、吞食和繁殖。

重要性：长角蜱（Haemaphysalis longicornis）是一种强制性吸血外寄生虫，因其传播医学和兽医学上重要的病原体而备受关注，是大韩民国最常见的蜱虫物种。控制蜱虫的首选策略是接种多种抗原疫苗。测试组合抗原的效率是制造蜱疫苗的一种有前途的方法：本研究旨在通过基因沉默分析亚olesin 和烯醇化酶在长角蜱摄食和繁殖中的作用：方法：在本研究中，我们使用 RNA 干扰来沉默长角蜱的唾液烯醇化酶和亚olesin。将注射了针对亚olesin和烯醇化酶的双链 RNA 的未进食雌蜱附着在兔子耳朵上并正常进食。使用实时聚合酶链反应确认基因敲除的程度：结果：亚olesin或烯醇化酶dsRNA组的蜱基因敲除率分别为80%和60%。组合 dsRNA（亚olesin 和烯醇化酶）组的蜱基因敲除率为 80%。亚叶绿素和烯醇化酶被敲除后，蜱的摄食量、吸血重量、附着率和产卵量都显著下降。沉默亚olesin和烯醇化酶会导致蜱啮合、产卵、卵孵化（15%）和繁殖显著减少（p < 0.05）：我们的研究结果表明，亚叶绿素和烯醇化酶是未来蜱虫控制策略的一个令人兴奋的目标。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Veterinary Science 农林科学-兽医学

CiteScore

3.10

自引率

5.60%

发文量

审稿时长

1.3 months

期刊介绍： The Journal of Veterinary Science (J Vet Sci) is devoted to the advancement and dissemination of scientific knowledge concerning veterinary sciences and related academic disciplines. It is an international journal indexed in the Thomson Scientific Web of Science, SCI-EXPANDED, Sci Search, BIOSIS Previews, Biological Abstracts, Focus on: Veterinary Science & Medicine, Zoological Record, PubMed /MEDLINE, Index Medicus, Pubmed Central, CAB Abstracts / Index Veterinarius, EBSCO, AGRIS and AGRICOLA. This journal published in English by the Korean Society of Veterinary Science (KSVS) being distributed worldwide.