Journal of Veterinary Science最新文献

Gut microbial assessment among Hylobatidae at the National Wildlife Rescue Centre, Peninsular Malaysia. 马来西亚半岛国家野生动物救护中心对虹彩蝠科动物的肠道微生物进行评估。

IF 1.5 3区农林科学

Journal of Veterinary Science Pub Date : 2024-08-16 DOI: 10.4142/jvs.23312

Roberta Chaya Tawie Tingga, Millawati Gani, Abd Rahman Mohd-Ridwan, Nor Rahman Aifat, Ikki Matsuda, Badrul Munir Md-Zain

{"title":"Gut microbial assessment among Hylobatidae at the National Wildlife Rescue Centre, Peninsular Malaysia.","authors":"Roberta Chaya Tawie Tingga, Millawati Gani, Abd Rahman Mohd-Ridwan, Nor Rahman Aifat, Ikki Matsuda, Badrul Munir Md-Zain","doi":"10.4142/jvs.23312","DOIUrl":"https://doi.org/10.4142/jvs.23312","url":null,"abstract":"Importance: Recent developments in genetic analytical techniques have enabled the comprehensive analysis of gastrointestinal symbiotic bacteria as a screening tool for animal health conditions, especially the endangered gibbons at the National Wildlife Rescue Centre (NWRC).Objective: High-throughput sequencing based on 16S ribosomal RNA genes was used to determine the baseline gut bacterial composition and identify potential pathogenic bacteria among three endangered gibbons housed in the NWRC.Methods: Feces were collected from 14 individuals (Hylobates lar, n = 9; Hylobates agilis, n = 4; and Symphalangus syndactylus, n = 1) from March to November 2022. Amplicon sequencing were conducted by targeting V3-V4 region.Results: The fecal microbial community of the study gibbons was dominated by Bacteroidetes and Firmicutes (phylum level), Prevotellaceae and Lachnospiraceae/Muribaculaceae (family level), and Prevotella (and its subgroups) (genera level). This trend suggests that the microbial community composition of the study gibbons differed insignificantly from previously reported conspecific or closely related gibbon species.Conclusions and relevance: This study showed no serious health problems that require immediate attention. However, relatively low alpha diversity and few potential bacteria related to gastrointestinal diseases and streptococcal infections were detected. Information on microbial composition is essential as a guideline to sustain a healthy gut condition of captive gibbons in NWRC, especially before releasing this primate back into the wild or semi-wild environment. Further enhanced husbandry environments in the NWRC are expected through continuous health monitoring and increase diversity of the gut microbiota through diet diversification.","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxidative hemolytic crises in a dog due to fragrance products: clinical insights and treatment approaches. 香料产品导致的犬氧化性溶血危机：临床见解和治疗方法。

IF 1.5 3区农林科学

Journal of Veterinary Science Pub Date : 2024-08-14 DOI: 10.4142/jvs.24160

Sully Lee, Kyoung-Won Seo, Urs Giger, Min-Ok Ryu

{"title":"Oxidative hemolytic crises in a dog due to fragrance products: clinical insights and treatment approaches.","authors":"Sully Lee, Kyoung-Won Seo, Urs Giger, Min-Ok Ryu","doi":"10.4142/jvs.24160","DOIUrl":"https://doi.org/10.4142/jvs.24160","url":null,"abstract":"Importance: This is the first reported case of fragrance products-induced recurrent oxidative hemolytic anemia in a dog, detailing the successful therapeutic approach employed.Case presentation: A 4-year-old intact female Pomeranian dog presented with brown tongue, pigmenturia, peripheral edema, and vomiting. Blood smears revealed a high count of eccentrocytes and Heinz bodies, along with a precipitous decline in packed cell volume and an increase in blood methemoglobin levels, suggesting an oxidative hemolytic crisis. This clinicopathological pattern recurred several times after the patient returned home. Antioxidants, methylene blue, hyperbaric oxygen (HBO) therapy, and blood transfusion were successfully employed to address recurrent hemolytic anemia; however, oxidative hemolytic crises recurred. After the owner removed exposure to various home remedies and fragrances, the clinical signs and hemolytic crises did not recur.Conclusion and relevance: Recurring oxidative hemolytic crises should raise suspicions of environmental toxicity, which, although harmless in small quantities to humans, can be devastating to small-breed dogs. In addition to removing the causative agents, methylene blue and other antioxidants, along with HBO, may be beneficial in the acute management of oxidative hemolytic anemia.","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Different development patterns of reward behaviors induced by ketamine and JWH-018 in striatal GAD67 knockdown mice. 氯胺酮和JWH-018诱导纹状体GAD67基因敲除小鼠奖赏行为的不同发展模式

IF 1.5 3区农林科学

Journal of Veterinary Science Pub Date : 2024-08-12 DOI: 10.4142/jvs.23325

Sun Mi Gu, Eunchong Hong, Sowoon Seo, Sanghyeon Kim, Seong Shoon Yoon, Hye Jin Cha, Jaesuk Yun

{"title":"Different development patterns of reward behaviors induced by ketamine and JWH-018 in striatal GAD67 knockdown mice.","authors":"Sun Mi Gu, Eunchong Hong, Sowoon Seo, Sanghyeon Kim, Seong Shoon Yoon, Hye Jin Cha, Jaesuk Yun","doi":"10.4142/jvs.23325","DOIUrl":"https://doi.org/10.4142/jvs.23325","url":null,"abstract":"Importance: Glutamic acid decarboxylase 67 (GAD67) is a gamma-aminobutyric acid (GABA) synthesis enzyme associated with the function of other neurotransmitter receptors, such as the N-methyl-D-aspartate (NMDA) receptor and cannabinoid receptor 1. However, the role of GAD67 in the development of different abused drug-induced reward behaviors remains unknown. In order to elucidate the mechanisms of substance use disorder, it is crucial to study changes in biomarkers within the brain's reward circuit induced by drug use.Objective: The study was designed to examine the effects of the downregulation of GAD67 expression in the dorsal striatum on reward behavior development.Methods: We evaluated the effects of GAD67 knockdown on depression-like behavior and anxiety using the forced swim test and elevated plus maze test in a mouse model. We further determined the effects of GAD67 knockdown on ketamine- and JWH-018-induced conditioned place preference (CPP).Results: Knockdown of GAD67 in the dorsal striatum of mice increased depression-like behavior, but it decreased anxiety. Moreover, the CPP score on the NMDA receptor antagonist ketamine was increased by GAD67 knockdown, whereas the administration of JWH-018, a cannabinoid receptor agonist, did not affect the CPP score in the GAD67 knockdown mice group compared with the control group.Conclusions and relevance: These results suggest that striatal GAD67 reduces GABAergic neuronal activity and may cause ketamine-induced NMDA receptor inhibition. Consequently, GAD67 downregulation induces vulnerability to the drug reward behavior of ketamine.","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Meat ducks as carriers of antimicrobial-resistant Escherichia coli harboring transferable R plasmids. 肉鸭是携带可转移 R 质粒的耐抗菌大肠杆菌的载体。

IF 1.5 3区农林科学

Journal of Veterinary Science Pub Date : 2024-08-12 DOI: 10.4142/jvs.24074

Zulqarnain Baqar, Nuananong Sinwat, Rangsiya Prathan, Rungtip Chuanchuen

{"title":"Meat ducks as carriers of antimicrobial-resistant Escherichia coli harboring transferable R plasmids.","authors":"Zulqarnain Baqar, Nuananong Sinwat, Rangsiya Prathan, Rungtip Chuanchuen","doi":"10.4142/jvs.24074","DOIUrl":"https://doi.org/10.4142/jvs.24074","url":null,"abstract":"Importance: Antimicrobial resistance (AMR) is a serious public health threat. AMR bacteria and their resistance determinants in food can be transmitted to humans through the food chain and by direct contact and disseminate directly to the environment.Objective: This study examined the AMR characteristics and transferable R plasmids in Escherichia coli isolated from meat ducks raised in an open-house system.Methods: One hundred seventy-seven (n = 177) commensal E. coli were examined for their antimicrobial susceptibilities and horizontal resistance transfer. The plasmids were examined by PCR-based plasmid replicon typing (PBRT) and plasmid multi-locus sequence typing (pMLST).Results: The highest resistance rate was found against ampicillin (AMP, 83.0%) and tetracycline (TET, 81.9%), and most isolates exhibited multidrug resistance (MDR) (86.4%). The R plasmids were conjugally transferred when TET (n = 4), AMP (n = 3), and chloramphenicol (n = 3) were used as a selective pressure. The three isolates transferred resistance genes either in AMP or TET. The blaCTX-M1 gene resided on conjugative plasmids. Five replicon types were identified, of which Inc FrepB was most common in the donors (n = 13, 38.4%) and transconjugants (n = 16, 31.2%). Subtyping F plasmids revealed five distinct replicons combinations, including F47:A-:B- (n = 2), F29:A-:B23 (n = 1), F29:A-:B- (n = 1), F18:A-B:- (n = 1), and F4:A-:B- (n = 1). The chloramphenicol resistance was significantly correlated with the other AMR phenotypes (p < 0.05).Conclusions and relevance: The meat ducks harbored MDR E. coli and played an important role in the environmental dissemination of AMR bacteria and its determinants. This confirms AMR as a health issue, highlighting the need for routine AMR monitoring and surveillance of meat ducks.","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of concurrent infection with Jaagsiekte sheep retrovirus and maedi-visna virus in China. 确定中国同时感染了Jaagsiekte羊逆转录病毒和maedi-visna病毒。

IF 1.5 3区农林科学

Journal of Veterinary Science Pub Date : 2024-08-08 DOI: 10.4142/jvs.24158

Xujie Duan, Xiaona Shi, Pei Zhang, Xiaoyue Du, Sixu Chen, Liang Zhang, Huiping Li, Yufei Zhang, Jinling Wang, Yulin Ding, Shuying Liu

{"title":"Identification of concurrent infection with Jaagsiekte sheep retrovirus and maedi-visna virus in China.","authors":"Xujie Duan, Xiaona Shi, Pei Zhang, Xiaoyue Du, Sixu Chen, Liang Zhang, Huiping Li, Yufei Zhang, Jinling Wang, Yulin Ding, Shuying Liu","doi":"10.4142/jvs.24158","DOIUrl":"https://doi.org/10.4142/jvs.24158","url":null,"abstract":"Importance: Ovine pulmonary adenomatosis (OPA) and maedi-visna disease (MVD) are chronic and progressive infectious diseases in sheep caused by Jaagsiekte sheep retrovirus (JSRV) and maedi-visna virus (MVV), respectively.Objective: To investigate the pathological changes and conduct viral gene analysis of OPA and MVD co-occurrence in Inner Mongolia, China.Methods: Using gross pathology, histopathology, immunohistochemistry, ultrastructural pathology, PCR, and sequence analysis, we investigated the concurrent infection of JSRV and MVV in 319 Dorper rams slaughtered in a private slaughterhouse in Inner Mongolia, in 2022.Results: Of the 319 rams included, 3 showed concurrent JSRV and MVV infection. Gross lung pathology showed diffuse enlargement, consolidation, and greyish-white miliary nodules on the lung surface; the trachea was filled with a white foamy fluid; hilar and mediastinal lymph nodes were significantly enlarged. Histopathology results revealed typical OPA and MVD lesions in the lung tissue. Immunohistochemical results were positive for JSRV envelope protein (Env) in the tumor cells and MVV CA in alveolar macrophages. Transmission electron microscopy showed several virions and autophagosomes in the lung tissue, severely damaged mitochondria, and the induced mitophagy. Nucleotide sequences obtained for JSRV env and MVV gag showed the highest homology with the Inner Mongolian strains of JSRV env (JQ837489) and MVV gag (MW248464).Conclusions and relevance: Our study confirmed that OPA and MVD co-occurrence and identified the pathological changes in Inner Mongolia, China, thereby providing references for the identification of concurrent JSRV and MVV infections.","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alzheimer disease-like neuropathologic changes in a geriatric baboon (Papio hamadryas). 老年狒狒（Papio hamadryas）的阿尔茨海默病样神经病理变化。

IF 1.5 3区农林科学

Journal of Veterinary Science Pub Date : 2024-08-07 DOI: 10.4142/jvs.24080

William T Harrison, J Mark Cline, David L Caudell, Hillary F Huber, Carol A Shively, Thomas C Register, Suzanne Craft, Jason D Struthers

{"title":"Alzheimer disease-like neuropathologic changes in a geriatric baboon (Papio hamadryas).","authors":"William T Harrison, J Mark Cline, David L Caudell, Hillary F Huber, Carol A Shively, Thomas C Register, Suzanne Craft, Jason D Struthers","doi":"10.4142/jvs.24080","DOIUrl":"https://doi.org/10.4142/jvs.24080","url":null,"abstract":"Importance: Alzheimer's disease (AD) is the most common cause of dementia in the elderly with the incidence rising exponentially after the age of 65 years. Unfortunately, effective treatments are extremely limited and definite diagnosis can only be made at autopsy. This is in part due to our limited understanding of the complex pathophysiology, including the various genetic, environmental, and metabolic contributing factors. In an effort to better understand this complex disease, researchers have employed nonhuman primates as translational models.Case presentation: This report aims to describe the AD-like neuropathology in the brain of a 37-year-old female baboon (Papio hamadryas), which at the time of her death made her the oldest hamadryas baboon at any member institution of the Association of Zoos and Aquariums. A diagnostic necropsy was performed, and the brain was evaluated for neurodegenerative disease. Frequent amyloid-β deposits were identified, consistent with what has been described in other geriatric nonhuman primates. Phospho-tau pathology, including neurofibrillary tangles, a feature not well-described in other primate models, was also abundant.Conclusions and relevance: Our results suggest that more detailed, prospective, longitudinal studies are warranted utilizing this particular species to see if they represent a viable model for human brain aging.","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of a new bovine picornavirus (Boosepivirus) in the Republic of Korea. 在大韩民国鉴定出一种新的牛皮卡病毒（Boosepivirus）。

IF 1.5 3区农林科学

Journal of Veterinary Science Pub Date : 2024-08-06 DOI: 10.4142/jvs.24148

Jeong-Byoung Chae, Seung-Uk Shin, Serim Kim, Hansong Chae, Won Gyeong Kim, Joon-Seok Chae, Hyuk Song, Jung-Won Kang

{"title":"Identification of a new bovine picornavirus (Boosepivirus) in the Republic of Korea.","authors":"Jeong-Byoung Chae, Seung-Uk Shin, Serim Kim, Hansong Chae, Won Gyeong Kim, Joon-Seok Chae, Hyuk Song, Jung-Won Kang","doi":"10.4142/jvs.24148","DOIUrl":"https://doi.org/10.4142/jvs.24148","url":null,"abstract":"Importance: Despite advancements in herd management, feeding, and pharmaceutical interventions, neonatal calf diarrhea (NCD) remains a major global health concern. Bacteria, viruses, and parasites are the major contributors to NCD. Although several pathogens have been identified in the Republic of Korea (ROK), the etiological agents of numerous NCD cases have not been identified.Objectives: To identify, for the first time, the prevalence and impact of Boosepivirus (BooV) on calf diarrhea in the ROK.Methods: Here, the unknown cause of calf diarrhea was determined using metagenomics We then explored the prevalence of certain pathogens, including BooV, that cause NCD. Seventy diarrheal fecal samples from Hanwoo (Bos taurus coreanae) calves were analyzed using reverse transcriptase and quantitative real-time polymerase chain reaction for pathogen detection and BooV isolate sequencing.Results: The complete genome of BooV was detected from unknown causes of calf diarrhea. And also, BooV was the most frequently detected pathogen (35.7%) among 8 pathogens in 70 diarrheic feces from Hanwoo calves. Co-infection analyses indicated that most BooV-positive samples were solely infected with BooV, indicating its significance in NCD in the ROK. All isolates were classified as BooV B in phylogenetic analysis.Conclusions and relevance: This is the first study to determine the prevalence and molecular characteristics of BooV in calf diarrhea in the ROK, highlighting the potential importance of BooV as a causative agent of calf diarrhea and highlighting the need for further research on its epidemiology and pathogenicity.","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142140456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of symmetric dimethylarginine as a serologic marker for kidney function in striped skunks (Mephitis mephitis). 将对称二甲基精氨酸作为条纹鼬（Mephitis mephitis）肾功能血清学标记的研究。

IF 1.5 3区农林科学

Journal of Veterinary Science Pub Date : 2024-07-01 DOI: 10.4142/jvs.24057

Eun Jung, Soong-Hee Youn, Ki-Yong Shin, Hyeon-Joo Shin, Joon-Young Yang, Yeseul Yang, Jae-Ha Jung, Yongbaek Kim

{"title":"Investigation of symmetric dimethylarginine as a serologic marker for kidney function in striped skunks (Mephitis mephitis).","authors":"Eun Jung, Soong-Hee Youn, Ki-Yong Shin, Hyeon-Joo Shin, Joon-Young Yang, Yeseul Yang, Jae-Ha Jung, Yongbaek Kim","doi":"10.4142/jvs.24057","DOIUrl":"10.4142/jvs.24057","url":null,"abstract":"Importance: Kidney disease is prevalent among veterinary species, including zoo animals; however, investigations into this condition in striped skunks (Mephitis mephitis) are scarce. Diagnostic tools for kidney diseases in this species also remain limited.Objective: This study aimed to assess the utility of symmetric dimethylarginine as a biomarker for kidney disease in captive striped skunks in Korea.Methods: This retrospective study analysed 11 striped skunks housed at the Everland Zoo between 2017 and 2021. Blood samples were collected during health checks. Kidney function was assessed through blood analysis and diagnostic ultrasound, with necropsies conducted on deceased animals. Symmetric dimethylarginine levels were measured in 27 plasma samples collected from 11 skunks.Results: Over the study period, seven skunks were diagnosed with kidney disease. Analysis of 27 blood samples revealed a concurrent increase in SDMA levels with concentrations of blood urea nitrogen and blood creatinine. In 3 of the 7 skunks with kidney disease, symmetric dimethylarginine exceeded 14 µg/dL prior to the elevation of blood urea nitrogen and blood creatinine above the upper reference limit.Conclusions and relevance: To our knowledge, this is the first study investigating symmetric dimethylarginine in captive striped skunks in Korea. Our findings suggest that symmetric dimethylarginine may serve as an early and consistent biomarker for renal dysfunction in striped skunks. Further studies with larger clinical sample size from striped skunks are needed to validate the clinical utility of blood symmetric dimethylarginine concentration.","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11291430/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NOX4 and its association with myeloperoxidase and osteopontin in regulating endochondral ossification. NOX4 及其与髓过氧化物酶和骨素在调节软骨内骨化过程中的关联。

IF 1.5 3区农林科学

Journal of Veterinary Science Pub Date : 2024-07-01 Epub Date: 2024-05-24 DOI: 10.4142/jvs.24076

Kayoung Ko, Seohee Choi, Miri Jo, Chaeyoung Kim, Napissara Boonpraman, Jihyun Youm, Sun Shin Yi

{"title":"NOX4 and its association with myeloperoxidase and osteopontin in regulating endochondral ossification.","authors":"Kayoung Ko, Seohee Choi, Miri Jo, Chaeyoung Kim, Napissara Boonpraman, Jihyun Youm, Sun Shin Yi","doi":"10.4142/jvs.24076","DOIUrl":"10.4142/jvs.24076","url":null,"abstract":"Importance: Endochondral ossification plays an important role in skeletal development. Recent studies have suggested a link between increased intracellular reactive oxygen species (ROS) and skeletal disorders. Moreover, previous studies have revealed that increasing the levels of myeloperoxidase (MPO) and osteopontin (OPN) while inhibiting NADPH oxidase 4 (NOX4) can enhance bone growth. This investigation provides further evidence by showing a direct link between NOX4 and MPO, OPN in bone function.Objective: This study investigates NOX4, an enzyme producing hydrogen peroxide, in endochondral ossification and bone remodeling. NOX4's role in osteoblast formation and osteogenic signaling pathways is explored.Methods: Using NOX4-deficient (NOX4-/-) and ovariectomized (OVX) mice, we identify NOX4's potential mediators in bone maturation.Results: NOX4-/- mice displayed significant differences in bone mass and structure. Compared to the normal Control and OVX groups. Hematoxylin and eosin staining showed NOX4-/- mice had the highest trabecular bone volume, while OVX had the lowest. Proteomic analysis revealed significantly elevated MPO and OPN levels in bone marrow-derived cells in NOX4-/- mice. Immunohistochemistry confirmed increased MPO, OPN, and collagen II (COLII) near the epiphyseal plate. Collagen and chondrogenesis analysis supported enhanced bone development in NOX4-/- mice.Conclusions and relevance: Our results emphasize NOX4's significance in bone morphology, mesenchymal stem cell proteomics, immunohistochemistry, collagen levels, and chondrogenesis. NOX4 deficiency enhances bone development and endochondral ossification, potentially through increased MPO, OPN, and COLII expression. These findings suggest therapeutic implications for skeletal disorders.","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11291435/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141442972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Outbreaks of nosocomial feline calicivirus-associated virulent systemic disease in Korea. 韩国爆发的与猫卡里科病毒相关的传染性全身性疾病。

IF 1.5 3区农林科学

Journal of Veterinary Science Pub Date : 2024-07-01 DOI: 10.4142/jvs.24030

Junghoon Park, Dohyun Lee, Yeon-Jung Hong, Cheol-Yong Hwang, Jae-Eun Hyun

{"title":"Outbreaks of nosocomial feline calicivirus-associated virulent systemic disease in Korea.","authors":"Junghoon Park, Dohyun Lee, Yeon-Jung Hong, Cheol-Yong Hwang, Jae-Eun Hyun","doi":"10.4142/jvs.24030","DOIUrl":"10.4142/jvs.24030","url":null,"abstract":"Importance: Feline calicivirus (FCV)-associated viral systemic disease (VSD) is a severe systemic disease caused by virulent FCV strains and has a very poor prognosis.Objective: To evaluate the clinical characteristics of a nosocomial FCV-VSD outbreak involving 18 cats in Korea.Methods: Medical records of cats diagnosed with FCV-VSD from March to September 2018 at a referral veterinary hospital were reviewed. The patient's signalment, history, clinical features, diagnosis, treatment, and prognosis were evaluated.Results: Two outbreaks involving 18 cats diagnosed with FCV-VSD occurred over a 6-month period at a referral hospital in Korea. Anorexia, lethargy, fever, and limb edema were the most commonly observed clinical symptoms. Lymphopenia and macrothrombocytopenia were the most common hematological findings, and hyperbilirubinemia and increased levels of aspartate aminotransferase, creatine kinase, and serum amyloid A were the most frequent results of serum biochemistry. FCV was detected by reverse transcription polymerase chain reaction in 11 patients and the remaining 7 were suspected with FCV-VSD. The overall mortality rate was 72.2%. The hospital was closed and disinfected twice, and no additional outbreaks have occurred since the last patient.Conclusions and relevance: The clinical and diagnostic characteristics and outcomes of FCV-VSD described in this study can be used to recognize and contain infectious diseases through quick action. To the best of the authors' knowledge, this is the first report of a nosocomial outbreak of FCV-VSD in Asia.","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11291428/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0