Journal of Veterinary Science最新文献

{"title":"Casein kinase 1 epsilon (CK1ε) as a potential therapeutic target in chronic liver disease.","authors":"Mwense Leya, Thach Phan Van, Jong-Won Kim, Bumseok Kim","doi":"10.4142/jvs.24321","DOIUrl":"10.4142/jvs.24321","url":null,"abstract":"<p><strong>Importance: </strong>Chronic liver disease (CLD) is a significant global health concern, often progressing to hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma in both humans and animals. Despite substantial research efforts, effective CLD treatments remain scarce. Casein kinase 1 epsilon (CK1ε), a serine/threonine kinase, plays a pivotal role in several critical signaling pathways, including the Wingless/Integrated (Wnt)/β-catenin, HIPPO, and mitogen-activated protein kinase (MAPK) pathways, all of which contribute to liver disease progression.</p><p><strong>Observations: </strong>CK1ε regulates key pathways that drive liver fibrosis, inflammation, and cancer. Its involvement in lipid metabolism and adipogenesis links CK1ε to metabolic dysfunctional-associated steatotic liver disease. Elevated CK1ε levels are observed in disease models beyond CLD, underscoring its broad role in pathological conditions. Moreover, CK1ε phosphorylates critical proteins such as Wnt/β-catenin, RAS/MAPK, phosphoinositide 3-kinase/protein kinase B, transcription coactivators yes-associated protein 1 and the PDZ-binding motif, and Sprouty homolog 2, suggesting potential influence on liver cell function and fibrosis development. Preclinical models demonstrate that CK1ε inhibitors, including PF-4800567, PF-670462, and IC261, effectively reduce tumor growth and fibrosis of variable etiologies.</p><p><strong>Conclusions and relevance: </strong>CK1ε's central role in liver disease progression makes it a compelling target for therapeutic strategies. Targeting CK1ε with small molecules or gene therapies could offer novel treatment avenues for CLD. However, challenges related to target specificity and safety must be addressed. Further research and translational studies could pave the way for precision medicine approaches, enhancing treatment outcomes for both animals and humans with CLD.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 3","pages":"e30"},"PeriodicalIF":1.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146023/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First case report of urothelial cyst in a dog: diagnostic approach.","authors":"Soobin Kim, Minjung Kang, Jihee Hong, Youngsuk Kim, Jaehwan Kim, Kidong Eom","doi":"10.4142/jvs.24338","DOIUrl":"10.4142/jvs.24338","url":null,"abstract":"<p><strong>Importance: </strong>Cystic masses in the abdominal cavity near the vaginal area can have a range of origins, including urothelial cysts, which have never been reported in dogs. This paper reports the first known occurrence of a urothelial cyst in a dog.</p><p><strong>Case presentation: </strong>An 18-year-old, 2.05 kg intact female Maltese dog presented with a mammary nodule with no systemic clinical signs. The radiographs and ultrasound revealed a large cystic mass in the abdominal cavity. Computed tomography imaging showed that the mass was in contact with the bladder, urethra, and vagina but had no direct connections to these organs. Surgical removal was performed. Histopathology analysis confirmed a urothelial cyst lined with transitional and stratified squamous epithelium.</p><p><strong>Conclusions and relevance: </strong>This case highlights the need to include urothelial cysts in a differential diagnosis of cystic masses near the vaginal region in dogs. Early imaging-based detection is crucial to preventing misdiagnoses and ensuring appropriate surgical intervention. Veterinarians must carefully differentiate this condition from other reproductive disorders, including pyometra and uterine tumors, to avoid complications and ensure appropriate management.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 3","pages":"e40"},"PeriodicalIF":1.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146022/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence, distribution, and risk factors of <i>Cryptosporidium</i> spp. infection among calves in the Far-North Region of Cameroon.","authors":"Augustin Siama, Justin Kalmobe, Rodrigue Simonet Poueme Namegni, Samuel Abah, Alexandre Michel Njan Nlôga","doi":"10.4142/jvs.24040","DOIUrl":"10.4142/jvs.24040","url":null,"abstract":"<p><strong>Importance: </strong>Cryptosporidiosis is a ubiquitous gastrointestinal zoonotic disease of young calves and children under 5 years of age, but it is relatively unknown in Cameroon.</p><p><strong>Objective: </strong>This study examined the prevalence, distribution, and risk factors of cryptosporidiosis in calves of the Far North Region of Cameroon.</p><p><strong>Methods: </strong>Fecal samples were collected and examined using the modified Ziehl-Neelsen staining technique for <i>Cryptosporidium</i> spp. oocysts.</p><p><strong>Results: </strong>A total of 261/629 calves tested positive for infection, giving an overall 41.5% prevalence. The prevalence was significantly higher in calves younger than 3 months (59.68%) (<i>p</i> < 0.000), with a body condition score (BCS) 2 (69.56%) (p<0.000), with diarrheic stools (74.72%), during the rainy season (74.72%) (<i>p</i> < 0.000), and in large herds (48.16%) (<i>p</i> = 0.046). This disease was distributed widely in all divisions and localities of the study. The risk of infection was higher in calves aged < 3 months (<i>p</i> < 0.000), with diarrheic stools (<i>p</i> < 0.000), BCS 2 (<i>p</i> < 0.000), during the rainy season (<i>p</i> < 0.000), and in large herds (<i>p</i> < 0.000). The prevalence of cryptosporidiosis was distributed widely in diarrhoeic calves (<i>p</i> < 0.000).</p><p><strong>Conclusions and relevance: </strong>This study provides the first evidence of <i>Cryptosporidium</i> spp. infection of calves in the Far North region of Cameroon with the associated risk factors such as age, BCS, season, herd size, diarrheal status, feed source, barn hygiene, and water sources. Further molecular investigation in this region will be needed to implement control strategies to limit zoonotic transmission to humans.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 3","pages":"e41"},"PeriodicalIF":1.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146028/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of visual dye-based loop-mediated isothermal amplification assays for <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> detection in ruminants.","authors":"Sudu Hakuruge Madusha Pramud Wimalasena, Sang Young Seo, Han Gyu Lee, Young Hun Jung, Yoon Jung Do, Ara Cho, Changyong Choe, Are Sun Yoo, Seogjin Kang, Jun Ho Lee, Han Sang Yoo, Eun-Yeong Bok","doi":"10.4142/jvs.24201","DOIUrl":"10.4142/jvs.24201","url":null,"abstract":"<p><strong>Importance: </strong><i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> (MAP), the causative agent of Johne's disease (JD) in ruminants, is a potential zoogenic pathogen. At the asymptomatic subclinical stage, infected animals periodically shed the pathogen through feces and milk, serving as silent carriers for disease transmission. In Republic of Korea, where treatment for JD is not pursued and culling is the primary management strategy for infected animals, early detection is crucial for effective disease control.</p><p><strong>Objective: </strong>In this study, we aimed to develop a new primer set for the loop-mediated isothermal amplification (LAMP) method for facilitating visual-based detection of MAP.</p><p><strong>Methods: </strong>We designed LAMP primers for the MAP-specific gene IS<i>1311</i> and optimized the reaction conditions to enhance diagnostic sensitivity and specificity. Practical application was evaluated using 170 field samples of feces and intestinal tissues from cattle and goats.</p><p><strong>Results: </strong>The LAMP method demonstrated sensitivity superior to that of the conventional polymerase chain reaction (PCR) method and equal to that of real-time-PCR targeting the F<i>57</i> gene, detecting as low as 0.1 pg MAP DNA per reaction. Notably, we integrated a mixed dye composed of calcein and hydroxynaphthol blue at an optimal ratio, enabling clear visual distinction of results under natural light. In practical application, the LAMP assay demonstrated a true positive rate of 100% and true-negative rate of 92.22%.</p><p><strong>Conclusions and relevance: </strong>The developed mixed dye-LAMP method facilitates immediate, sensitive diagnosis by providing a user-friendly readout. By reducing diagnostic time and enhancing visual clarity without specialized equipment, it significantly improves JD control in the livestock industry.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 3","pages":"e31"},"PeriodicalIF":1.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146026/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of tissue tropism of chicken anaemia virus on chicken embryos: a molecular insight.","authors":"Sedeneinuo Suohu, Gurusamipalayam Amirthalingam Balasubramaniam, Ayyasamy Arulmozhi, Thippichettipalayam Ramasamy Gopalakrishnamurthy, Angamuthu Raja","doi":"10.4142/jvs.24186","DOIUrl":"10.4142/jvs.24186","url":null,"abstract":"<p><strong>Importance: </strong>Few studies have examined the interactions of the chicken anemia virus (CAV) with the immune system of embryos.</p><p><strong>Objective: </strong>To study the pathogenicity of the CAV in chicken embryos with respect to its tissue tropism and viral load in different tissues.</p><p><strong>Method: </strong>The pathogenicity of the CAV was examined in about-to-hatch embryos through cytology, histopathological examinations, molecular detection, immunocytochemistry (ICC), immunofluorescent test, and immunohistochemistry techniques.</p><p><strong>Results: </strong>The experimental study using a polymerase chain reaction (PCR) assay revealed the presence of CAV in the liver, bone marrow, and thymus tissues of embryos where embryos were inoculated with CAV-positive bone marrow specimens obtained from chicken anemia-suspect cases. Intracytoplasmic and intranuclear inclusion bodies were detected cytologically in the bone marrow of the embryos, whereas no inclusion body was detected in the thymus. On the other hand, both kinds of inclusion bodies were observed in bone marrow and thymus in the histopathology. In addition, various organs of PCR-positive CAV embryos revealed the depletion of lymphocytes and hematopoietic cells in the thymus and bone marrow, respectively. The CAV antigen was confirmed in the bone marrow and thymus cytological smears using ICC and immunofluorescence techniques.</p><p><strong>Conclusions and relevance: </strong>Viral antigen exhibited more tropism towards the bone marrow of embryos than the thymus in ICC and immunofluorescence techniques. The presence of CD44, a marker for progenitor cells and an antigen in the serial sections of bone marrow, confirmed the relevance of viral tropism for the hemocytoblasts of the bone marrow as the main target cells in embryos and subsequently in chicks for viral replication.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 3","pages":"e17"},"PeriodicalIF":1.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146021/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Baicalin protects neurons from oxidative stress and apoptosis induced by glutamate excitotoxicity in HT-22 cells.","authors":"Hyun-Kyoung Son, Phil-Ok Koh","doi":"10.4142/jvs.24334","DOIUrl":"10.4142/jvs.24334","url":null,"abstract":"<p><strong>Importance: </strong>Baicalin is a flavonoid compound with various functions, including neuroprotective effects. Glutamate is an essential excitatory neurotransmitter involved in synaptic transmission, but it can also induce neuronal damage through excitotoxicity.</p><p><strong>Objective: </strong>To analyze the anti-oxidant and anti-apoptotic effects of baicalin on glutamate-exposed neuronal cells.</p><p><strong>Methods: </strong>Mouse neuronal hippocampal HT-22 cells were cultured and treated with glutamate (5 mM) and/or baicalin (10, 30, 50 µM). Baicalin was administered 1 h before glutamate treatment, and cells were collected 24 h following glutamate exposure. Reactive oxygen species (ROS) and lipid peroxidation (LPO) analyses were performed to determine the oxidative stress. Western blot and immunocytochemical staining were performed to investigate the expressions of bcl-2, bax, and caspase-3.</p><p><strong>Results: </strong>Glutamate induced severe neuronal damage, including cell morphological condensation, which was attenuated by baicalin treatment. Baicalin treatment ameliorated the decrease in cell viability due to glutamate toxicity. Baicalin mitigated glutamate-induced increase of ROS and LPO in a dose-dependent manner. Glutamate exposure induced the downregulation of bcl-2 and the upregulation of bax, thereby reducing the bcl-2 to bax ratio, while administration of baicalin prevented these changes. Baicalin treatment ameliorated the glutamate toxicity-induced increase in caspase-3.</p><p><strong>Conclusions and relevance: </strong>Baicalin exerts antioxidant and anti-apoptotic functions against glutamate toxicity in neurons by preventing oxidative stress and inhibiting the apoptotic pathway.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 3","pages":"e29"},"PeriodicalIF":1.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146029/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Imported case of canine viscerocutaneous leishmaniasis in South Korea: clinical presentation and diagnostic approach in a Labrador Retriever.","authors":"Ki-Yeon Son, Gyeong-Gook Park, Joong-Hyun Song","doi":"10.4142/jvs.24296","DOIUrl":"10.4142/jvs.24296","url":null,"abstract":"<p><strong>Importance: </strong>Leishmaniasis, a sandfly-borne disease, can infect both humans and dogs, with dogs acting as key reservoirs. This report documents the first case of leishmaniasis in South Korea, identified in a dog imported from Spain. It highlights the importance for early detection and careful monitoring of dogs imported from endemic regions to prevent the introduction and spread of leishmaniasis to regions such as South Korea, where the disease is rare.</p><p><strong>Case presentation: </strong>A 2-year-old male Labrador Retriever dog, imported from Spain at 12 months old, was presented with a 5-month history of generalized cutaneous lesions and a 1-month history of chronic diarrhea. Microscopic examination of peripheral blood smears revealed amastigote-infected macrophages and whole-blood polymerase chain reaction confirmed a diagnosis of viscerocutaneous leishmaniasis. The dog was treated with allopurinol, the only available treatment for leishmaniasis in South Korea, alongside supportive management. However, the patient showed a poor response to treatment.</p><p><strong>Conclusions and relevance: </strong>This case of canine leishmaniasis in South Korea highlights the growing risk of imported infections in non-endemic areas and underscores the need for greater awareness and understanding of the clinical features of Leishmaniasis for timely diagnosis and management in dogs with a history of travel or adoption from endemic regions.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 3","pages":"e33"},"PeriodicalIF":1.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146019/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safety and efficacy of a <i>Bordetella bronchiseptica</i> outer membrane proteins (OMPs) subunit vaccine in a murine model.","authors":"Ji Young Jang, Byung Joon Park, Huiyeon Song, Seungshik Rha, Hyoung Joon Moon, Han Sang Yoo","doi":"10.4142/jvs.25018","DOIUrl":"10.4142/jvs.25018","url":null,"abstract":"<p><strong>Importance: </strong><i>Bordetella bronchiseptica</i> is a major pathogen responsible for respiratory infections in dogs, posing significant challenges to companion animal health. The whole-cell vaccine currently available has various adverse effects, and concerns persist regarding the efficacy of acellular vaccines. Therefore, developing a safer and more effective vaccine is critical for addressing these issues.</p><p><strong>Objective: </strong>This study evaluated the safety and efficacy of <i>B. bronchiseptica</i> outer membrane proteins (OMPs) and dermonecrotic toxin (DNT) as subunit vaccine candidates, individually and in combination, compared to conventional vaccine formulations in murine models.</p><p><strong>Methods: </strong>The immune responses and side effects were assessed after vaccination with OMPs and DNT as subunit vaccine candidates, individually and in combination. The safety evaluations included monitoring the body temperature, weight, and vitality scores, while the efficacy was measured using immunoglobulin quantification, hemagglutination-inhibition (HI) assays, and challenge tests. The vaccine candidates were compared with conventional vaccine formulations.</p><p><strong>Results: </strong>The OMP-based vaccine induced a T helper 1-biased immune response, providing robust protection against <i>B. bronchiseptica</i> with minimal adverse effects. More than 90% disease protection was achieved at OMP concentrations of 25-50 µg/mL, with immunoglobulin G levels exceeding 2¹³ (8,192) and HI titers surpassing 2^7.5 (181). In contrast, commercially available Bordetella vaccine formulations, which contain cellular wall components and toxins, showed reduced efficacy and heightened side effects.</p><p><strong>Conclusions and relevance: </strong>OMPs are a promising, cost-effective component for <i>B. bronchiseptica</i> vaccine development, providing a balance between safety and efficacy. These insights could promote the development of improved Bordetella vaccines for dogs and potentially humans, addressing the longstanding trade-offs in current vaccine formulations and contributing to better animal health.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 3","pages":"e39"},"PeriodicalIF":1.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146018/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methodological evaluation of ultrasound-guided modified traumatic catheterization in dogs with suspected urothelial carcinoma.","authors":"Jisu Lee, Ul Soo Choi, Minji Kwon, Hakyoung Yoon","doi":"10.4142/jvs.24308","DOIUrl":"10.4142/jvs.24308","url":null,"abstract":"<p><strong>Importance: </strong>Bladder tumors in dogs, particularly urothelial carcinoma (UC), pose a diagnostic challenge due to overlapping imaging features with benign conditions. Accurate diagnosis is crucial for appropriate treatment and prognosis.</p><p><strong>Objective: </strong>To assess the effectiveness of a modified traumatic catheterization technique using a 4-hole catheter for cytological sampling in dogs with bladder masses.</p><p><strong>Methods: </strong>Fifteen dogs with hematuria and mass-like bladder lesions identified using ultrasonography were included in the study. 15 dogs underwent modified traumatic catheterization using a four-hole catheter created by adding two extra holes to a 6 or 8 Fr feeding tube. The catheter was inserted into the bladder, and cells were aspirated from the lesion under ultrasound guidance. The procedure's efficacy was evaluated both quantitatively and qualitatively.</p><p><strong>Results: </strong>All 15 cases achieved a 100% cell acquisition rate with high cellularity. The median visualization score was 3/3, the manipulation score was 3/3, and the cellularity score was 2/2. The average procedure time was 8.8 min. Diagnoses included 12 cases of UC and three cases of polypoid cystitis/urinary bladder polyps. No significant adverse effects, including bladder perforation or urethral trauma, were observed. Mild bleeding occurred in some cases but resolved spontaneously.</p><p><strong>Conclusions and relevance: </strong>Modified traumatic catheterization using a 4-hole catheter is a safe, efficient, and minimally invasive technique for diagnosing UC and other bladder conditions. It is particularly useful for small-sized dogs and those unsuitable for invasive procedures. The enhanced catheter design improves lesion contact and sampling efficiency by offering a valuable tool for veterinary practitioners.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 3","pages":"e32"},"PeriodicalIF":1.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146027/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perioperative use of flash glucose monitoring system in dogs undergoing general surgery: a clinical feasibility study.","authors":"Seung-Yeon Yu, Hakhyun Kim, Min Jang, Sungin Lee","doi":"10.4142/jvs.24271","DOIUrl":"10.4142/jvs.24271","url":null,"abstract":"<p><strong>Importance: </strong>Flash Glucose Monitoring System (FGMS; FreeStyle Libre) enables non-invasive, continuous glucose monitoring. However, its perioperative utility in canine surgeries has not been evaluated. This study highlights the feasibility of FGMS in perioperative glucose management for veterinary patients.</p><p><strong>Objective: </strong>This study aimed to evaluate the clinical applicability and accuracy of FGMS in perioperative glucose monitoring in dogs.</p><p><strong>Methods: </strong>Thirty-two dogs that underwent surgery were monitored during the preoperative, intraoperative, and postoperative phases using FGMS and portable blood glucose meter (PBGM).</p><p><strong>Results: </strong>A total of 224 paired glucose measurements were analyzed for correlation and accuracy using ISO 15197:2013 standards. Spearman's correlation analysis revealed positive correlation between FGMS and PBGM (ρ = 0.894, ρ = 0.823, and ρ = 0.795, respectively) during the preoperative, intraoperative, and postoperative phases, respectively (<i>p</i> < 0.001). The Bland-Altman plot of the difference between FGMS and PBGM showed a positive bias during the intraoperative phase (23.3 mg/dL), despite a negative bias in the preoperative (-16.0 mg/dL) and postoperative (-4.58 mg/dL) phases. Parkes consensus error grid analysis demonstrated that 100% of the data pairs fell within clinically acceptable zones (zones A and B) throughout the operation.</p><p><strong>Conclusions and relevance: </strong>FGMS provides continuous and noninvasive glucose monitoring in dogs with clinical feasibility throughout the perioperative period, although its accuracy decreases during the intraoperative phase. For patients with relatively stable blood glucose levels, the Libre device is clinically effective for perioperative glucose monitoring and provides a simpler and more accessible alternative to traditional methods.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 3","pages":"e23"},"PeriodicalIF":1.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146024/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}