Journal of Veterinary Science最新文献

{"title":"Multimodal MRI findings in a dog with suspected global brain ischemia and peri-anesthetic blindness.","authors":"Hye-Won Lee, Yoonhoi Koo, Kija Lee, Sang-Kwon Lee","doi":"10.4142/jvs.26132","DOIUrl":"https://doi.org/10.4142/jvs.26132","url":null,"abstract":"<p><strong>Importance: </strong>Global brain ischemia (GBI) in dogs is diagnosed based on acute neurologic signs, temporal association with a hypoxic-ischemic or anesthetic event, and supportive magnetic resonance imaging (MRI) findings. Cortical T2-weighted (T2W) and fluid-attenuated inversion recovery (FLAIR) hyperintensity and diffusion restriction are described, but diagnosis may be challenging when these findings are absent. We describe a dog with peri-anesthetic acute bilateral blindness in which conventional MRI was equivocal, but advanced MRI supported suspected GBI by demonstrating global hypoperfusion, reduced arterial conspicuity, and microstructural alterations.</p><p><strong>Case presentation: </strong>A 10-year-old intact male Welsh Corgi was noted to have acute bilateral blindness at discharge after overnight hospitalization following general anesthesia at a local hospital and later deteriorated neurologically. On examination, mentation was depressed; menace responses were absent bilaterally with intact pupillary light reflexes. Brain MRI revealed diffuse cerebral swelling with sulcal effacement and no conspicuous supratentorial T2W or FLAIR hyperintensity or clear diffusion restriction. Advanced MRI supported suspected GBI: arterial spin labeling showed markedly reduced cerebral blood flow (CBF) in the cortex and thalamus; dynamic susceptibility contrast showed reduced relative CBF, increased relative cerebral blood volume, and prolonged transit metrics; time-of-flight magnetic resonance angiography showed poor intracranial arterial conspicuity; and diffusion tensor imaging demonstrated cortical alterations in fractional anisotropy and diffusivity metrics. Mannitol and supportive care were administered; blindness persisted and the dog was discharged with poor neurologic recovery.</p><p><strong>Conclusions and relevance: </strong>Multimodal MRI may support the diagnosis of canine GBI when conventional MRI is equivocal and may characterize global hemodynamic and microstructural injury.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149111605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic-guided precision oncology in canine cancer: from comparative genomics to liquid biopsy and molecular monitoring.","authors":"Minjeong Kang, Jaemin Kim, Jinho Park, DoHyeon Yu","doi":"10.4142/jvs.25370","DOIUrl":"10.4142/jvs.25370","url":null,"abstract":"<p><strong>Importance: </strong>Traditional cancer diagnostics in veterinary medicine are limited by invasiveness and insufficient sensitivity for real-time molecular monitoring. This review critically evaluates the clinical applicability of somatic mutation profiling and liquid biopsy technologies to establish an evidence-based, tiered framework for precision oncology in canine patients, with emphasis on veterinary-specific constraints and technology selection.</p><p><strong>Observations: </strong>Key somatic biomarkers demonstrate strong clinical utility: BRAF V595E achieves > 80% diagnostic sensitivity in canine urothelial carcinoma and is detectable non-invasively in urine via droplet digital polymerase chain reaction (ddPCR). The multicenter cancer detection in dogs (CANDiD) study demonstrated circulating tumor DNA-based liquid biopsy achieves 54.7% overall sensitivity and 98.5% specificity, though stage I detection remains critically limited at 16.1%, underscoring the current gap for early-stage screening. Minimal residual disease assessment via allele-specific oligonucleotide-PCR and next-generation sequencing enables detection of molecular relapse 42-168 days before clinical manifestation in canine lymphoma. However, analytical variability across platforms, lack of standardized pipelines, and high cost represent significant barriers to routine veterinary application.</p><p><strong>Conclusions and relevance: </strong>Genomic-guided oncology offers substantial clinical value for canine cancer management yet requires veterinary-specific validation before broad adoption. Targeted sequencing panels currently represent the optimal balance of cost-effectiveness and clinical utility for most veterinary practitioners, while ddPCR is preferred for longitudinal tracking of known mutations. Liquid biopsy cannot serve as a standalone screening tool at present, but functions as a valuable complementary monitoring approach. Continued canine-specific biomarker research and multicenter data sharing are essential prerequisites for integrating precision medicine into routine veterinary care.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"27 5","pages":"e53"},"PeriodicalIF":1.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13655552/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149136895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arthroscopy in specific joint pathologies of dogs and potential for application in wildlife species.","authors":"Young Deok Suh, Soeun Ryu, Do Na Lee, Seong Chan Yeon","doi":"10.4142/jvs.26015","DOIUrl":"10.4142/jvs.26015","url":null,"abstract":"<p><strong>Importance: </strong>Arthroscopy has become an essential minimally invasive technique for both diagnostic and surgical applications in veterinary medicine. However, despite its established clinical utility in domestic small animals, particularly for the evaluation and management of arthropathies in major joints of dogs, its application in wildlife species remains limited. Expanding the use of arthroscopy in wildlife species may address important gaps in the diagnosis and management of arthropathies across diverse taxa.</p><p><strong>Observations: </strong>This narrative review demonstrates that arthroscopy is extensively utilized in small animals, especially dogs, for both diagnostic and therapeutic management of major joint disorders, supported by continuous developments in instrumentation and procedural techniques. In contrast, arthroscopic applications in wildlife species are largely confined to isolated case reports, with considerable variability in anatomical feasibility, surgical approaches, and clinical outcomes, leading to a limited understanding of disease pathophysiology, treatment options, and clinical outcomes in non-domestic species. This disparity highlights a substantial gap in the evidence base regarding the use of arthroscopy across non-domestic species.</p><p><strong>Conclusions and relevance: </strong>Arthroscopy provides a versatile and effective modality in veterinary orthopedics, with well-established applications in canine patients. However, its use in wildlife medicine remains limited and inconsistent. Continued refinement of arthroscopic equipment and techniques, along with the accumulation of clinical experience, may facilitate broader adoption in wildlife species. This review provides a timely overview of current practices and highlights clinically relevant gaps, supporting future research and potential clinical application in non-domestic species.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":" ","pages":"e52"},"PeriodicalIF":1.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13655556/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148865535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Catecholamine-associated left ventricular hypertrophy in dogs with hyperadrenocorticism and myxomatous mitral valve disease: a prospective pilot study.","authors":"Hwi Park, Jung-Hyun Kim","doi":"10.4142/jvs.26013","DOIUrl":"10.4142/jvs.26013","url":null,"abstract":"<p><strong>Importance: </strong>Endogenous Cushing's syndrome increases the risk of cardiac hypertrophy, dysfunction, and cardiovascular mortality. Given the high prevalence of hyperadrenocorticism (HAC) and myxomatous mitral valve disease (MMVD) in dogs, endocrine-cardiac interactions may influence disease progression but remain poorly characterized.</p><p><strong>Objective: </strong>To evaluate the effects of concurrent HAC on cardiac remodeling and function in dogs with subclinical MMVD and identify metabolic and neurohormonal markers, particularly catecholamine metabolites, associated with left ventricular hypertrophy and dysfunction.</p><p><strong>Methods: </strong>Twenty-one client-owned dogs (HAC + MMVD, MMVD-only controls, and healthy controls [each n = 7]) were prospectively enrolled. Echocardiographic variables, including speckle-tracking echocardiography, hematologic and biochemical parameters, and urinary normetanephrine: creatinine ratios, were evaluated at baseline and follow-up.</p><p><strong>Results: </strong>The HAC + MMVD group showed significantly increased urinary normetanephrine: creatinine ratios than both healthy control group and MMVD-only control group (<i>p</i> = 0.011 and <i>p</i> = 0.007, respectively). Urinary normetanephrine: creatinine ratio was independently associated with interventricular septum wall thickness in diastole (IVSdN, B = 0.000222, 95% confidence interval [CI], 0.000050 to 0.000393, <i>p</i> = 0.026). Δ Urinary normetanephrine: creatinine was positively associated with Δ IVSdN after adjusting for Δ days (B = 0.000308, 95% CI, 0.000108 to 0.000507, <i>p</i> = 0.016). Global longitudinal strain (GLS) was lower in the HAC + MMVD group than both the healthy control group and the MMVD-only control group (<i>p</i> = 0.001 and <i>p</i> = 0.002, respectively). However, GLS was not associated with normetanephrine levels (r = 0.179, 95% CI, -0.698 to 1.000, <i>p</i> = 0.720).</p><p><strong>Conclusions and relevance: </strong>This exploratory pilot study is the first suggesting a potential neuroendocrine mechanism underlying endocrine-cardiac remodeling in dogs with concurrent HAC and MMVD, whereby increased catecholamine activity may be associated with left ventricular hypertrophy.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":" ","pages":"e67"},"PeriodicalIF":1.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13655568/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148865511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of an open-access canine breed-associated neurologic disease literature library.","authors":"Kathryn Dalzell, Avinash Bevoor, Rebecca Wilson, Rodney S Bagley","doi":"10.4142/jvs.25316","DOIUrl":"10.4142/jvs.25316","url":null,"abstract":"<p><strong>Importance: </strong>The Veterinary Neurologic Disease Library has been created as an educational resource library for veterinary professionals related to breed-associated neurologic diseases in dogs.</p><p><strong>Objective: </strong>To develop an open-access library that serves as a curated collection repository of peer-reviewed literature pertaining to dog breeds and neurologic diseases.</p><p><strong>Methods: </strong>Dog breeds were identified using the American and United Kennel Club registered breed lists. Disease terms were collected from published texts discussing veterinary neurologic diseases. Publicly available resources were searched by inputting keywords, including dog breeds, specific neurologic diseases, clinical signs, and additional related terms. Retrieved information was stored in a Zotero web-library, and automated searches continue to be conducted monthly. Original peer-reviewed manuscripts (in English) were categorized for inclusion with an individual unique breed was identified with a neurologic disease.</p><p><strong>Results: </strong>The library is currently active and accessible online. Four hundred breeds have been included for peer-reviewed citations of neurologic diseases. At the time of this writing, over 1,900 scholarly citations have been identified and categorized.</p><p><strong>Conclusions and relevance: </strong>This centralized, open-access, repository of breed-associated neurologic diseases can be accessed to search breed and neurologic disease to assist primary care clinicians in the identification breed-associated neurologic disease in dogs and locating original source publications.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":" ","pages":"e59"},"PeriodicalIF":1.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13655558/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148865558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-informed structural analysis of polymerase and glycoprotein adaptation in H5N1 clade 2.3.4.4b.","authors":"Sangyi Lee, Seunghye Cho, Hyunbeen Kim, Woojin Shin, Soyoung Lee, Atanas V Demirev, Jin Il Kim","doi":"10.4142/jvs.26088","DOIUrl":"10.4142/jvs.26088","url":null,"abstract":"<p><strong>Importance: </strong>Understanding the molecular mechanisms driving H5N1 clade 2.3.4.4b is critical for pandemic preparedness.</p><p><strong>Objective: </strong>To characterize the molecular drivers of viral fitness and mammalian adaptability in recent H5N1 viruses by integrating evolutionary dynamics with structural simulations.</p><p><strong>Methods: </strong>This study analyzed 2,398 H5Nx genomes (2000-2024) through phylogenetic and selective pressure analyses. HA/NA structures were predicted with AlphaFold 3 and evaluated by AutoDock4 docking, whereas polymerase-ANP32A/B complexes were modeled using template-based methods and their binding free energies were estimated using MM/GBSA. Polymerase-ANP32E complexes were predicted with AlphaFold 3 and similarly evaluated by MM/GBSA. The binding affinities (ΔG) for the sialic acid (SA) receptors and human ANP32 proteins were quantified through molecular mechanics/generalized born surface area calculations.</p><p><strong>Results: </strong>Clade 2.3.4.4b showed significant antigenic drift in the HA receptor binding site, reducing affinity for <i>α</i>2,3-SA and <i>α</i>2,6-SA receptors. On the other hand, the emergence of a full-length stalk N1 NA with second sialic acid-binding site mutations (e.g., N366S) compensated for reduced HA affinity by enhancing the NA binding stability. In the polymerase complex, both the PB2-627E/631L variant (-144.00 kcal/mol; unadjusted <i>p</i> = 0.0058) and the known mammalian-adaptive 627K/631M variant (-144.67 kcal/mol; unadjusted <i>p</i> = 0.0165) showed more favorable predicted human ANP32B binding free energies than the ancestral 627E/631M state (-136.46 kcal/mol).</p><p><strong>Conclusions and relevance: </strong>The co-occurrence of HA, NA, PB1, and PB2 signatures was associated with clade expansion and produced structural predictions consistent with altered receptor or ANP32 interactions; experimental validation is required before inferring effects on fitness or zoonotic risk.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":" ","pages":"e65"},"PeriodicalIF":1.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13655574/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148865505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Milbemycin oxime and lotilaner for heartworm prevention in dogs in Korea: a randomized controlled field trial.","authors":"Jinyeong Park, Subin Lee, Chongho Kim, Kyung-Duk Min, Hakhyun Kim, Seung-Hun Lee","doi":"10.4142/jvs.26072","DOIUrl":"10.4142/jvs.26072","url":null,"abstract":"<p><strong>Importance: </strong>Heartworm disease can lead to chronic cardiopulmonary complications in dogs, and treatment is often prolonged and costly. In Korea, <i>Dirofilaria immitis</i>, the causative agent of canine heartworm disease, has been reported in both dogs and mosquitoes and is generally considered endemic.</p><p><strong>Objective: </strong>This field study evaluated the effectiveness and safety of a combination of milbemycin oxime and lotilaner (Credelio Plus) for the prevention of heartworm disease in client-owned dogs in the Republic of Korea.</p><p><strong>Design/setting/patients: </strong>This randomized, single-blind, controlled field trial was conducted between June 2023 and February 2024. Client-owned dogs that were at least 8 weeks old, weighed more than 2.0 kg, were walked more than 3 times per week, and tested negative for heartworm at baseline were enrolled.</p><p><strong>Interventions: </strong>The dogs were allocated in a 1:1 ratio to receive either the investigational veterinary product (milbemycin oxime/lotilaner) or the control veterinary product (milbemycin oxime/afoxolaner) for six consecutive monthly treatments (days 0, 30, 60, 90, 120, and 150).</p><p><strong>Main outcomes and measures: </strong>Prevention of <i>D. immitis</i> infection was assessed monthly from days 0 to 180 using a commercial antigen test kit and conventional polymerase chain reaction. Safety was evaluated using body weight, physical examination findings, hematology and serum chemistry analyses, and adverse event monitoring to assess general health status and identify potential treatment-related abnormalities during the study period.</p><p><strong>Results: </strong>A total of 53 dogs were enrolled in the study, of which 52 were included in effectiveness and safety analyses. All dogs remained negative for <i>D. immitis</i> during the study period. In the safety evaluation, no statistically significant changes were observed in body weight, physical examination findings, or hematology and serum chemistry analyses following milbemycin oxime/lotilaner administration (<i>p</i> > 0.05). No adverse events were observed.</p><p><strong>Conclusions and relevance: </strong>The results of this study support the use of milbemycin oxime/lotilaner combination as a safe and effective option for the prevention of canine heartworm infections under field conditions in Korea. These findings indicate that this combination product may be a useful option in veterinary parasite control.</p><p><strong>Trial registration: </strong>Animal and Plant Quarantine Agency, Korea (clinical trial number: A221512).</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":" ","pages":"e61"},"PeriodicalIF":1.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13655557/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148865517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylodynamic analysis provides insights into genotype displacement of duck hepatitis A virus in China.","authors":"Chenyi Xie, Baoyan Wang, Ying Wang, Guangrong Zhao, Yang Liu, Jianlong Zhang, Xin Yu, Linlin Jiang, Jiayu Yu, Jiuyu Zhan, Hongwei Zhu, Xingxiao Zhang","doi":"10.4142/jvs.25210","DOIUrl":"10.4142/jvs.25210","url":null,"abstract":"<p><strong>Importance: </strong>Duck viral hepatitis, caused by the duck hepatitis A virus (DHAV), poses a significant threat to the global duck industry, particularly in China.</p><p><strong>Objective: </strong>This study examined the evolutionary mechanisms driving the genotype displacement of DHAV-1 by DHAV-3 in China using phylodynamic analysis.</p><p><strong>Methods: </strong>The <i>vp1</i> genes of 9 DHAV-1 and 17 DHAV-3 strains were sequenced and combined with 82 DHAV-1 and 86 DHAV-3 sequences from GenBank. Bayesian phylogenetic, demographic, and phylogeographic analyses were performed using BEAST v1.10.</p><p><strong>Results: </strong>The substitution rate for DHAV-1 <i>vp1</i> was 4.89 × 10⁻⁴ substitutions/site/year (95% highest posterior density [HPD]: 3.072 × 10⁻⁴ to 6.83 × 10⁻⁴), and for DHAV-3 was 2.125 × 10⁻⁴ substitutions/site/year (95% HPD: 1.686 × 10⁻⁴ to 2.567 × 10⁻⁴). The time to the most recent common ancestor for DHAV-1 was estimated at ~1911 and for DHAV-3 at ~1992. The demographic trends showed a decline in DHAV-1 after 2003 and a resurgence of DHAV-3 in ~2010. Phylogeography identified China as the hub for DHAV-3 spread to South Korea, Vietnam, and Egypt.</p><p><strong>Conclusions and relevance: </strong>DHAV-3 has a higher evolutionary rate, and adaptive mutations drive its dominance, informing vaccine design and control strategies.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"27 5","pages":"e69"},"PeriodicalIF":1.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13655562/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149137180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antiplatelet effect of spironolactone via modulation of cPLA₂-mediated thromboxane A₂ generation in murine platelets.","authors":"Sanggu Kim, Preeti Kumari Chaudhary, Seongmo Yang, Soochong Kim","doi":"10.4142/jvs.26033","DOIUrl":"10.4142/jvs.26033","url":null,"abstract":"<p><strong>Importance: </strong>Spironolactone, a steroidal aldosterone antagonist, is widely used as a diuretic and antihypertensive agent. Steroids have been known to promote thrombosis by directly increasing platelet count and activation and indirectly by increasing oxidative stress and suppressing nitric oxide synthesis. However, the effects of spironolactone on platelet activation remain unclear.</p><p><strong>Objective: </strong>To investigate the effects of spironolactone on platelet function and underlying signaling mechanisms.</p><p><strong>Methods: </strong>Platelet aggregation, dense granule secretion, thromboxane A₂ (TxA₂) generation, and protein phosphorylation were evaluated in platelets treated with spironolactone.</p><p><strong>Results: </strong>Spironolactone significantly inhibited the secondary wave of platelet aggregation and dense granule secretion induced by 2-methylthioadenosine diphosphate (2-MeSADP). It is known that 2-MeSADP-induced secretion and the resultant secondary wave of aggregation depend on TxA₂-mediated positive feedback, suggesting a role for spironolactone in regulating TxA₂ generation in platelets. Additionally, spironolactone did not affect 2-MeSADP-induced platelet aggregation in aspirin-treated platelets in which TxA₂ generation was blocked. Furthermore, platelet aggregation and secretion induced by low concentrations of thrombin, which are also affected by TxA₂-mediated positive feedback, were partially inhibited by spironolactone. Notably, spironolactone significantly suppressed TxA₂ generation induced by both 2-MeSADP and thrombin. Finally, spironolactone markedly inhibited the 2-MeSADP- and thrombin-induced phosphorylation of cytosolic phospholipase A₂ (cPLA₂), extracellular signal-regulated kinase, and protein kinase B in non-aspirin-treated platelets, whereas only cPLA₂ phosphorylation was significantly inhibited in aspirin-treated platelets.</p><p><strong>Conclusions and relevance: </strong>Spironolactone inhibits platelet activation by suppressing TxA₂ generation, primarily by modulating cPLA₂ phosphorylation, providing mechanistic insight into its effects on platelet signaling <i>in vitro</i>.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"27 5","pages":"e72"},"PeriodicalIF":1.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13655564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149137369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive analysis of the prevalence and influencing factors of felid alphaherpesvirus-1 (FHV-1) in mainland China from 2017 to 2024.","authors":"Jiayu Shi, Huiling Zhang, Xinyuan Liu, Xueping Zhang, Hongyu Zhou, Tangjie Zhang","doi":"10.4142/jvs.25184","DOIUrl":"10.4142/jvs.25184","url":null,"abstract":"<p><strong>Importance: </strong>Felid alphaherpesvirus 1 (FHV-1) is a common cause of feline upper respiratory disease, but prevalence estimates from mainland China are fragmented.</p><p><strong>Objectives: </strong>To estimate pooled FHV-1 prevalence and evaluate factors associated with between-study variation in mainland China from 2017 through 2024.</p><p><strong>Data resources: </strong>PubMed, Web of Science, China National Knowledge Infrastructure, Wanfang Data, and the Chinese Journal Service Platform were searched; reference lists were also screened. Add the final search date.</p><p><strong>Study selection: </strong>Cross-sectional studies from mainland China that reported a diagnostic method and sufficient numerator and denominator data were eligible. Two reviewers screened studies independently, with disagreements resolved by discussion or a third reviewer.</p><p><strong>Data extraction and synthesis: </strong>Thirty studies comprising 17,108 samples were included. Pooled prevalence was estimated with a random-effects model after Freeman-Tukey double-arcsine transformation. Heterogeneity, subgroup differences, publication bias, and leave-one-out sensitivity were assessed.</p><p><strong>Main outcomes and measures: </strong>Pooled FHV-1 prevalence with 95% confidence intervals (CIs), heterogeneity, and subgroup estimates by age, sex, breed, season, publication period, and region.</p><p><strong>Results: </strong>Overall pooled prevalence was 23% (95% CI, 18%-29%; I² = 98.6%; heterogeneity <i>p</i> < 0.001). Prevalence was highest in cats aged 6 months or younger (51%; 95% CI, 26%-75%; subgroup <i>p</i> = 0.046) and in Exotic Shorthair cats (63%; 95% CI, 52%-74%; subgroup <i>p</i> = 0.003). Estimates differed by region (<i>p</i> = 0.021) but not by publication period (<i>p</i> = 0.742).</p><p><strong>Conclusions and relevance: </strong>FHV-1 prevalence in mainland China was substantial and highly heterogeneous. Age, breed, and region were associated with variation across studies. Targeted prevention and surveillance may be warranted, but the very high heterogeneity and study-level subgroup analyses limit causal inference.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"27 5","pages":"e46"},"PeriodicalIF":1.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13655567/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149137411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}