Detection and genetic evolution analysis of porcine reproductive and respiratory syndrome virus in Chengdu from January 2021 to December 2022.

IF 1.5 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Veterinary Science Pub Date : 2025-05-08 DOI:10.4142/jvs.24277

Hongyu Zhong, Kerong Li, Min Li, Kun Zhang, Yi Zhang, Luqi Liang, Haifeng Liu, Zhijun Zhong, Guangneng Peng, Xiaoxiao Zhou, Ziyao Zhou

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引用次数: 0

Abstract

Importance: Pandemics involving the porcine reproductive and respiratory syndrome virus (PRRSV) impact swine health and productivity, causing economic devastation, especially in large-scale feeding areas.

Objective: This study examined the prevalence and genetic variations of PRRSV in Chengdu, China, a vital transportation hub and the main area of pig breeding in southwest China, between January 2021 and December 2022.

Methods: The samples were collected from 13 key pig-feeding areas in Chengdu. The positive PRRSV samples were detected using a real-time quantitative polymerase chain reaction. The genetic variations of the PRRSV were analyzed by amplifying the open reading frame 5 (ORF5) gene and comparing it with relevant reference sequences.

Results: The PRRSV positive rate in Chengdu was 11.42% (276/2,416), including sublines 8.7, 5.1, 3.0, and 1.8. The ORF5 gene sequences in the samples had no insertion or deletion of amino acids but widespread variations, including the 13th and 151st positions, which were associated with viral virulence.

Conclusions and relevance: The PRRSV pandemic in Chengdu was relevant, and recombination between vaccine strains and field strains can contribute to vaccine immune failure.

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2021年1月- 2022年12月成都市猪繁殖与呼吸综合征病毒检测及遗传进化分析

重要性：涉及猪繁殖与呼吸综合征病毒（PRRSV）的大流行影响猪的健康和生产力，造成经济破坏，特别是在大规模饲养地区。目的：研究2021年1月至2022年12月，中国西南地区重要的交通枢纽和养猪主要地区成都PRRSV的流行情况和遗传变异。方法：在成都市13个重点养猪区采集样本。采用实时定量聚合酶链反应检测PRRSV阳性样本。通过扩增开放阅读框5 （ORF5）基因，并与相关参考序列进行比较，分析PRRSV的遗传变异。结果：成都市PRRSV阳性率为11.42%(276/ 2416)，分别为8.7、5.1、3.0、1.8亚型。样品中的ORF5基因序列没有插入或删除氨基酸，但存在广泛的变异，包括与病毒毒力相关的第13位和第151位。结论及相关性：此次成都PRRSV大流行具有相关性，疫苗毒株与田间毒株的重组可能导致疫苗免疫失败。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Veterinary Science 农林科学-兽医学

CiteScore

3.10

自引率

5.60%

发文量

审稿时长

1.3 months

期刊介绍： The Journal of Veterinary Science (J Vet Sci) is devoted to the advancement and dissemination of scientific knowledge concerning veterinary sciences and related academic disciplines. It is an international journal indexed in the Thomson Scientific Web of Science, SCI-EXPANDED, Sci Search, BIOSIS Previews, Biological Abstracts, Focus on: Veterinary Science & Medicine, Zoological Record, PubMed /MEDLINE, Index Medicus, Pubmed Central, CAB Abstracts / Index Veterinarius, EBSCO, AGRIS and AGRICOLA. This journal published in English by the Korean Society of Veterinary Science (KSVS) being distributed worldwide.