Journal of Veterinary Science最新文献

{"title":"Minimally traumatic extraction of fractured bilateral maxillary canine teeth using a piezoelectric surgical unit in an African lion (<i>Panthera leo</i>).","authors":"Se Eun Kim, Yoocheol Jeong","doi":"10.4142/jvs.24099","DOIUrl":"10.4142/jvs.24099","url":null,"abstract":"<p><strong>Importance: </strong>Canine extraction of large carnivores can pose significant risk due to extensive tissue damage during aggressive bone reduction. This report highlights a rare instance in which the use of a piezoelectric surgical unit (PSU) for maxillary canine extraction in a large carnivore resulted in successful outcomes with minimal tissue damage.</p><p><strong>Case presentation: </strong>A 10-year-old male African lion presented with decreased appetite because of bilateral maxillary canine fractures. Intraoral radiographs revealed enlarged root canals and periapical radiolucency of the fractured canines, leading to a diagnosis of periapical periodontitis and pulpitis. To extract the right maxillary canine, conventional method using hand instrument failed to achieve adequate luxation, necessitating the use of the flat blade of the PSU to sever the periodontal ligament. The left maxillary canine was extracted using PSU from the beginning, and the extraction time was markedly shortened by using PSU without additional alveolar bone damage or bleeding.</p><p><strong>Conclusion and relevance: </strong>This case demonstrated that utilizing PSU for canine extraction in a lion resulted in periodontal ligament separation, reducing damage to the alveolar bone and shortening surgical time. It suggests the promising application of PSU in tooth extraction for large wild animals, indicating its potential significance in veterinary dentistry.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11291438/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141442971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brain invasion of bovine coronavirus: molecular analysis of bovine coronavirus infection in calves with severe pneumonia and neurological signs.","authors":"Semaha Gul Yilmaz, Ozge Aydin, Hasan Emre Tali, Gizem Karadag, Kivilcim Sonmez, Erhan Bayraktar, Aysun Yilmaz, Nuri Turan, Zihni Mutlu, Munir Iqbal, Jurgen A Richt, Huseyin Yilmaz","doi":"10.4142/jvs.23261","DOIUrl":"10.4142/jvs.23261","url":null,"abstract":"<p><strong>Importance: </strong>Although the role of bovine coronavirus (BCoV) in calf diarrhea and respiratory disorders is well documented, its contribution to neurological diseases is unclear.</p><p><strong>Objective: </strong>This study conducted virological investigations of calves showing diarrhea and respiratory and neurological signs.</p><p><strong>Methods: </strong>An outbreak of diarrhea, respiratory, and neurological disorders occurred among the 12 calves in July 2022 in Istanbul, Türkiye. Two of these calves exhibited neurological signs and died a few days after the appearance of symptoms. One of these calves was necropsied and analyzed using molecular and histopathological tests.</p><p><strong>Results: </strong>BCoV RNA was detected in the brain, lung, spleen, liver, and intestine of the calf that had neurological signs by real-time reverse transcription polymerase chain reaction. Immunostaining was also observed in the intestine and brain. A 622 bp S1 gene product was noted on gel electrophoresis only in the brain. Phylogenetic analysis indicated that the BCoV detected in this study had a high proximity to the BCoV strain GIb with 99.19% nucleotide sequence homology to the strains detected in Poland, Israel, Türkiye, and France. No distinct genetic lineages were observed when the brain isolate was compared with the respiratory and enteric strains reported to GenBank. In addition, the highest identity (98,72%) was obtained with the HECV 4408 and L07748 strains of human coronaviruses.</p><p><strong>Conclusions and relevance: </strong>The strain detected in a calf brain belongs to the GIb-European lineage and shares high sequence homology with BCoV strains detected in Europe and Israel. In addition, the similarity between the human coronaviruses (4408 and L07748) raises questions about the zoonotic potential of the strains detected in this study.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11291437/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141442969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative proteomic analysis of PK-15 cells infected with wild-type strain and its EP0 gene-deleted mutant strain of pseudorabies virus.","authors":"Di Wang, Dongjie Chen, Shengkui Xu, Fang Wei, Hongyuan Zhao","doi":"10.4142/jvs.24069","DOIUrl":"10.4142/jvs.24069","url":null,"abstract":"<p><strong>Importance: </strong>As one of the main etiologic agents of infectious diseases in pigs, pseudorabies virus (PRV) infections have caused enormous economic losses worldwide. EP0, one of the PRV early proteins (EP) plays a vital role in PRV infections, but the mechanisms are unclear.</p><p><strong>Objective: </strong>This study examined the function of EP0 to provide a direction for its in-depth analysis.</p><p><strong>Methods: </strong>In this study, the EP0-deleted PRV mutant was obtained, and Tandem Mass Tag-based proteomic analysis was used to screen the differentially expressed proteins (DEPs) quantitatively in EP0-deleted PRV- or wild-type PRV-infected porcine kidney 15 cells.</p><p><strong>Results: </strong>This study identified 7,391 DEPs, including 120 and 21 up-regulated and down-regulated DEPs, respectively. Western blot analysis confirmed the changes in the expression of the selected proteins, such as speckled protein 100. Comprehensive analysis revealed 141 DEPs involved in various biological processes and molecular functions, such as transcription regulator activity, biological regulation, and localization.</p><p><strong>Conclusions and relevance: </strong>These results holistically outlined the functions of EP0 during a PRV infection and might provide a direction for more detailed function studies of EP0 and the stimulation of lytic PRV infections.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11291433/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological, and molecular investigation of <i>Canine parvovirus-2</i> infection in Egypt.","authors":"Eman Farag Ammar, Yamen Mohammed Hegazy, Magdy Al-Gaabary, Samah M Mosad, Mohamed Salem, Mohamed Marzok, Fadhel Housawi, Mohamed Al-Ali, Abdulrahman Alhaider, Amin Tahoun","doi":"10.4142/jvs.23270","DOIUrl":"10.4142/jvs.23270","url":null,"abstract":"<p><strong>Importance: </strong>Canine parvovirus enteritis (CPE) is a contagious viral disease of dogs caused by the canine parvovirus-2 (CPV-2) associated with high morbidity and mortality rates. CPV-2 has a high global evolutionary rate. Molecular characterization of CPV-2 and understanding its epidemiology are essential for controlling CPV-2 infections.</p><p><strong>Objective: </strong>This study examined the risk factors and survival outcomes of dogs infected with CPV-2. Molecular characterization of CPV-2 genotypes circulating in Egypt was performed to determine the evolution of CPV-2 nationally and globally.</p><p><strong>Methods: </strong>An age-matched case-control study was conducted on 47 control and 47 CPV-infected dogs. Conditional logistic regression analysis examined the association between the potential risk factors and CPE in dogs. Survival analysis was performed to determine the survival pattern of the infected dogs. Thirteen fecal samples from infected dogs were collected to confirm the CPV genotype by CPV-2 VP2 gene sequencing, assembly of nucleotide sequences, and phylogenic analysis.</p><p><strong>Results: </strong>Unvaccinated and roamer dogs had eight and 2.3 times higher risks of CPV infection than vaccinated dogs and non-roamer dogs, respectively. The risk of death from CPE was high among dogs without routine visits to veterinary clinics and among non-roamer dogs. Molecular characterization of CPV-2 confirmed its genotype identity and relationship with the CPV-2 c and b clade types.</p><p><strong>Conclusions and relevance: </strong>This study highlights the potential factors for CPE control, especially vaccination and preventing dogs from roaming freely outside houses. Isolated CPV genotypes are closely related to southern Asian genotypes, suggesting a substantial opportunity for global transmission.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11291436/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Imaging guided adrenalectomy with Indocyanine green fluorescence in a dog with a pheochromocytoma.","authors":"Seung-Yeon Yu, Sungin Lee","doi":"10.4142/jvs.24146","DOIUrl":"10.4142/jvs.24146","url":null,"abstract":"<p><strong>Importance: </strong>This case report presents the successful surgical removal of a pheochromocytoma in a dog using indocyanine green (ICG) fluorescence imaging.</p><p><strong>Case presentation: </strong>A 10-year-old, 6.87 kg, spayed female poodle was referred for an abdominal mass and eight episodes of vomiting. Computed tomography revealed masses in the spleen and left adrenal gland. Based on the plasma normetanephrine levels, the patient was diagnosed with pheochromocytoma. Subsequently, a total splenectomy and left adrenalectomy were performed. A dose of 5 mg/kg ICG was administered intravenously 24 h prior to surgery. Using ICG allowed visualization of tumor margins, aiding in complete resection and minimizing anesthesia-related risks.</p><p><strong>Conclusions and relevance: </strong>No evidence of recurrence or metastasis has been observed for 12 months. To our knowledge, this is the first report of the surgical removal of a pheochromocytoma using intraoperative ICG imaging in veterinary medicine. This case highlights the efficacy of ICG fluorescence imaging as a valuable tool for the surgical management of adrenal tumors in veterinary medicine.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11291434/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence and risk factors of sheep and goat pox virus in selected districts of Wolaita Zone, Southern Ethiopia.","authors":"Fentaye Kassa, Haben Fesseha, Mesfin Mathewos, Selenat Getachew, Nato Hundessa, Saliman Aliye, Isayas Asefa Kebede","doi":"10.4142/jvs.24086","DOIUrl":"10.4142/jvs.24086","url":null,"abstract":"<p><strong>Importance: </strong>Sheep and goat pox (SGP) virus infection is a highly fatal viral infection of small ruminants that causes major production losses in sheep and goats in Ethiopia while also limiting international trade.</p><p><strong>Objective: </strong>This study aimed to estimate the seroprevalence of SGP infection and assess related risk variables.</p><p><strong>Methods: </strong>A cross-sectional study was conducted from February to August 2023 on 384 serum samples taken from sheep and goats. A serum neutralization test was conducted to detect the presence of antibodies against the SGP virus in Wolaita Sodo Regional Laboratory.</p><p><strong>Results: </strong>The overall seroprevalence rate of SGP was 4.95%. Factors such as sheep (8.26%), female sheep and goats (7.45%), older sheep and goats (8.33%), larger flock size of sheep and goats (10.47%), poorly conditioned sheep and goats (31.58%), sheep and goats with a tick on their skin (10.38%), and animals that had not been vaccinated (5.17%) were found to have higher seroprevalence. Furthermore, the seropositivity in sheep was five times greater than in goats (adjusted odds ratio [AOR], 4.73; 95% confidence interval [CI], 1.39-15.99). Additionally, large-sized flocks of sheep and goats were more likely to be seropositive to pox disease than small-sized flocks (AOR, 6.73; 95% CI, 1.58-28.67).</p><p><strong>Conclusions and relevance: </strong>Thus, the study revealed the prevalence of SGP in the Wolaita zone. Additional research should be conducted to estimate the extent of the disease at the regional level, and management measures should be implemented to reduce the economic losses associated with this condition.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11291429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Salmonella species from poultry slaughterhouses in South Korea: carry-over transmission of <i>Salmonella</i> Thompson ST292 in slaughtering process.","authors":"Yewon Cheong, Jun Bong Lee, Se Kye Kim, Jang Won Yoon","doi":"10.4142/jvs.24053","DOIUrl":"10.4142/jvs.24053","url":null,"abstract":"<p><strong>Importance: </strong><i>Salmonella</i> outbreaks linked to poultry meat have been reported continuously worldwide. Therefore, <i>Salmonella</i> contamination of poultry meats in slaughterhouses is one of the critical control points for reducing disease outbreaks in humans.</p><p><strong>Objective: </strong>This study examined the carry-over contamination of <i>Salmonella</i> species through the entire slaughtering process in South Korea.</p><p><strong>Methods: </strong>From 2018 to 2019, 1,097 samples were collected from the nine slaughterhouses distributed nationwide. One hundred and seventeen isolates of <i>Salmonella</i> species were identified using the <i>invA</i> gene-specific polymerase chain reaction, as described previously. The serotype, phylogeny, and antimicrobial resistance of isolates were examined.</p><p><strong>Results: </strong>Among the 117 isolates, 93 were serotyped into <i>Salmonella</i> Mbandaka (n = 36 isolates, 30.8%), <i>Salmonella</i> Thompson (n = 33, 28.2%), and <i>Salmonella</i> Infantis (n = 24, 20.5%). Interestingly, allelic profiling showed that all <i>S</i>. Mbandaka isolates belonged to the lineage of the sequence type (ST) 413, whereas all <i>S</i>. Thompson isolates were ST292. Moreover, almost all <i>S</i>. Thompson isolates (97.0%, 32/33 isolates) belonging to ST292 were multidrug-resistant and possessed the major virulence genes whose products are required for full virulence. Both serotypes were distributed widely throughout the slaughtering process. Pulsed-field gel electrophoretic analysis demonstrated that seven <i>S</i>. Infantis showed 100% identities in their phylogenetic relatedness, indicating that they were sequentially transmitted along the slaughtering processes.</p><p><strong>Conclusions and relevance: </strong>This study provides more evidence of the carry-over transmission of <i>Salmonella</i> species during the slaughtering processes. ST292 <i>S</i>. Thompson is a potential pathogenic clone of <i>Salmonella</i> species possibly associated with foodborne outbreaks in South Korea.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11156591/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141247505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current strategies using 3D organoids to establish <i>in vitro</i> maternal-embryonic interaction.","authors":"Islam Mohamed Saadeldin, Seif Ehab, Ahmed Elsayed Noreldin, Ayman Abdel-Aziz Swelum, Seonggyu Bang, Hyejin Kim, Ki Young Yoon, Sanghoon Lee, Jongki Cho","doi":"10.4142/jvs.24004","DOIUrl":"10.4142/jvs.24004","url":null,"abstract":"<p><strong>Importance: </strong>The creation of robust maternal-embryonic interactions and implantation models is important for comprehending the early stages of embryonic development and reproductive disorders. Traditional two-dimensional (2D) cell culture systems often fail to accurately mimic the highly complex <i>in vivo</i> conditions. The employment of three-dimensional (3D) organoids has emerged as a promising strategy to overcome these limitations in recent years. The advancements in the field of organoid technology have opened new avenues for studying the physiology and diseases affecting female reproductive tract.</p><p><strong>Observations: </strong>This review summarizes the current strategies and advancements in the field of 3D organoids to establish maternal-embryonic interaction and implantation models for use in research and personalized medicine in assisted reproductive technology. The concepts of endometrial organoids, menstrual blood flow organoids, placental trophoblast organoids, stem cell-derived blastoids, and in vitro-generated embryo models are discussed in detail. We show the incorportaion of organoid systems and microfluidic technology to enhance tissue performance and precise management of the cellular surroundings.</p><p><strong>Conclusions and relevance: </strong>This review provides insights into the future direction of modeling maternal-embryonic interaction research and its combination with other powerful technologies to interfere with this dialogue either by promoting or hindering it for improving fertility or methods for contraception, respectively. The merging of organoid systems with microfluidics facilitates the creation of sophisticated and functional organoid models, enhancing insights into organ development, disease mechanisms, and personalized medical investigations.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11156602/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141247754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of respondents' knowledge, attitudes, and practices toward rabies and associated risk factors in Shone Town, Southern Ethiopia.","authors":"Teketel Gizaw Beresa, Teshita Edaso Beriso, Tesfaye Bekele Kassa, Isayas Asefa Kebede","doi":"10.4142/jvs.23301","DOIUrl":"10.4142/jvs.23301","url":null,"abstract":"<p><strong>Importance: </strong>Rabies is a neglected tropical viral disease most often transmitted through the bite of an infected animal.</p><p><strong>Objective: </strong>This study assessed the level of knowledge, attitudes, and practices of the Shone Town community toward rabies.</p><p><strong>Methods: </strong>A survey-based cross-sectional study was conducted in Shone town, Ethiopia, from November 2022 to April 2023. Woreda was selected purposefully, while Kebeles and the study populations were selected by simple random sampling. Four hundred and sixteen respondents were interviewed using a semi-structured questionnaire.</p><p><strong>Results: </strong>All respondents had heard about rabies from different sources, with the majority hearing from informal sources (62%). Approximately 51.9%, 0.7%, and 47.4% of individuals were aware of saliva contact, rabid animal bites, and both as means of transmission, respectively. The survey showed that 64.4% of participants knew the 100% fatal nature of rabies once the clinical signs developed, and 35.6% did not. Approximately 51.4% of respondents agreed that killing stray dogs was an effective method for rabies prevention. In this study, 72.6% of the respondents had contact with pets, and 36.8% of the interviewees had vaccinated their dogs. Only the educational level (<i>p</i> = 0.03) was associated with knowledge of the transmission route. Age (<i>p</i> = 0.04) and educational level (<i>p</i> = 0.01) had a statistically significant association with knowledge of the risk of not vaccinating dogs.</p><p><strong>Conclusions and relevance: </strong>A lack of formal education in the communities, low levels of education, and the majority of respondents acquiring their knowledge from unofficial sources are important contributors to the low levels of awareness.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11156595/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141247498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pulmonary passage of canine adipose tissue-derived mesenchymal stem cells through intravenous transplantation in mouse model.","authors":"Jaeyeon Kwon, Mu-Young Kim, Soojung Lee, Jeongik Lee, Hun-Young Yoon","doi":"10.4142/jvs.23300","DOIUrl":"10.4142/jvs.23300","url":null,"abstract":"<p><strong>Importance: </strong>The intravenous administration of adipose tissue-derived mesenchymal stem cells (AdMSCs) in veterinary medicine is an attractive treatment option. On the other hand, it can result in severe complications, including pulmonary thromboembolism (PTE).</p><p><strong>Objective: </strong>The present study assessed the occurrence of PTE after the intravenous infusion of canine AdMSCs (cAdMSCs) into experimental animals.</p><p><strong>Methods: </strong>Five-week-old male BALB/c hairless mice were categorized into groups labeled A to G. In the control group (A), fluorescently stained 2 × 10⁶ cAdMSCs were diluted in 200 μL of suspension and injected into the tail vein as a single bolus. The remaining groups included the following: group B with 5 × 10⁶ cells, group C with 3 × 10⁶ cells, group D with 1 × 10⁶ cells, group E with 1 × 10⁶ cells injected twice with a one-day interval, group F with 2 × 10⁶ cells in 100 μL of suspension, and group G with 2 × 10⁶ cells in 300 μL of suspension.</p><p><strong>Results: </strong>Group D achieved a 100% survival rate, while none of the subjects in groups B and C survived (<i>p</i> = 0.002). Blood tests revealed a tendency for the D-dimer levels to increase as the cell dose increased (<i>p</i> = 0.006). The platelet count was higher in the low cell concentration groups and lower in the high cell concentration groups (<i>p</i> = 0.028). A histological examination revealed PTE in most deceased subjects (96.30%).</p><p><strong>Conclusions and relevance: </strong>PTE was verified, and various variables were identified as potential contributing factors, including the cell dose, injection frequency, and suspension volume.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11156597/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141247852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}