Journal of Veterinary Science最新文献_第3页

Immuno-protective effect of commercial IBD vaccines against emerging novel variant infectious bursal disease virus in specific-pathogen-free chickens. 商用法氏囊病疫苗对新出现的新型变异传染性法氏囊病病毒的免疫保护作用。

IF 1.5 3区农林科学

Journal of Veterinary Science Pub Date : 2024-09-01 DOI: 10.4142/jvs.23297

Hayatuddeen Bako Aliyu, Tasiu Mallam Hamisu, Mohd Hair-Bejo, Abdul Rahman Omar, Aini Ideris

{"title":"Immuno-protective effect of commercial IBD vaccines against emerging novel variant infectious bursal disease virus in specific-pathogen-free chickens.","authors":"Hayatuddeen Bako Aliyu, Tasiu Mallam Hamisu, Mohd Hair-Bejo, Abdul Rahman Omar, Aini Ideris","doi":"10.4142/jvs.23297","DOIUrl":"10.4142/jvs.23297","url":null,"abstract":"Importance: Infectious bursal disease (IBD) is an important viral poultry disease that vaccination can control.Objective: This study examined the immune protection of immune-complex (Vaccine A) and attenuated live (Vaccine B) IBD vaccines in specific-pathogen-free (SPF) chickens against a novel Malaysian variant IBD virus (vaIBDV) challenge.Methods: One-day-old (n =75) SPF chickens were divided randomly into the following three groups of 25 chicks each: Control, Vaccine A, and Vaccine B groups. The vaIBDV strain, UPM1432/2019, was used for the challenge at 21 and 28days post-vaccination (dpv). Five birds from unchallenged and challenged groups were sacrificed seven days post-challenge, and blood, bursa, spleen, and cloacal swabs were collected. The IBD antibodies (Abs), lymphoid lesions, and viral load were determined.Results: The UPM1432/2019 virus induced bursal damage in vaccinated SPF chickens despite Ab titers. The mean Ab titers of the Vaccine A challenged group were significantly lower (p < 0.002) than in the unchallenged group at 28 dpv. The bursal indices of the vaccinated unchallenged groups did not differ significantly from those of the vaccinated challenged groups (p = 0.94). Microscopically, the bursae of the challenged groups showed significant atrophy. The bursal lesion score was higher (p < 0.05) in the control and Vaccine B challenged groups than the Vaccine A challenged group. The challenged group had a higher viral load than the vaccinated groups (p < 0.001).Conclusions and relevance: Neither vaccine fully protected against a vaIBDV challenge, highlighting the limitations of current vaccines and the need for further research.","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"25 5","pages":"e70"},"PeriodicalIF":1.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11450385/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142372212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characteristics and antimicrobial susceptibility profiles of bovine mastitis agents in western Türkiye. 图尔基耶西部牛乳腺炎病原体的分子特征和抗菌药敏感性概况。

IF 1.5 3区农林科学

Journal of Veterinary Science Pub Date : 2024-09-01 DOI: 10.4142/jvs.24032

Semiha Yalcin, Arzu Ozgen, Metehan Simsir

{"title":"Molecular characteristics and antimicrobial susceptibility profiles of bovine mastitis agents in western Türkiye.","authors":"Semiha Yalcin, Arzu Ozgen, Metehan Simsir","doi":"10.4142/jvs.24032","DOIUrl":"10.4142/jvs.24032","url":null,"abstract":"Importance: Identifying bovine mastitis agents using molecular methods to reveal their phylogenetic relationships and antimicrobial resistance profiles is essential for developing up-to-date databases in mastitis cases that cause severe economic losses.Objective: This study examined bacterial mastitis agents in cows with clinical and subclinical mastitis observed in various dairy cattle farms to reveal their phylogenetic relationships and antibiotic resistance properties.Methods: Sixty-two clinical and subclinical bovine mastitis milk samples were collected from 15 dairy farms. The polymerase chain reaction (PCR) was used to amplify the 16S rRNA gene regions of the bacteria. The 16S rRNA gene sequences obtained from sequencing include the V4-V6 regions. The strains were compared using a similarity analysis method that produced phylogenetic trees using the Molecular Evolutionary Genetics Analysis 11 program. Antibiotic susceptibilities were determined using the Kirby-Bauer disk diffusion method.Results: Sixty-three bacteria were isolated and identified in this study. The most isolated bacteria from all mastitis cases were Staphylococcus spp. (30.2%), Escherichia coli (25.4%), Streptococcus spp. (14.3%), and Aerococcus spp. (7.9%), respectively. The phylogenetic trees were drawn from the 16S rRNA sequences. Some of these bacteria showed resistance to different types of antibiotics at varying rates.Conclusions and relevance: The bacteria isolated in this study originated from environmental sources. Regular cleaning of barns and proper hygiene practices are essential. Regular screenings for mastitis should be conducted in herds instead of the random or empirical use of antibiotics.","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"25 5","pages":"e72"},"PeriodicalIF":1.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11450396/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142372215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developing veterinary basic clinical skill items based on Korean Veterinary Entrustable Professional Activity. 根据韩国兽医委托专业活动开发兽医基本临床技能项目。

IF 1.5 3区农林科学

Journal of Veterinary Science Pub Date : 2024-09-01 DOI: 10.4142/jvs.24129

Kichang Lee, Heungshik S Lee, Seong Mok Jeong, Jongil Kang, Seungjoon Kim, Jin-Young Chung, Sang-Soep Nahm, Pan Dong Ryu

{"title":"Developing veterinary basic clinical skill items based on Korean Veterinary Entrustable Professional Activity.","authors":"Kichang Lee, Heungshik S Lee, Seong Mok Jeong, Jongil Kang, Seungjoon Kim, Jin-Young Chung, Sang-Soep Nahm, Pan Dong Ryu","doi":"10.4142/jvs.24129","DOIUrl":"10.4142/jvs.24129","url":null,"abstract":"Importance: Developing clinical skills is an essential element of veterinary education to ensure the competency of veterinary graduates. Although the Korean Veterinary Education Graduation Competencies were established in 2016, reflecting domestic needs and international trends in competency-based veterinary education, they have yet to be implemented in Korean veterinary education.Objective: This study aimed to establish the basic veterinary clinical skills required to ensure graduates of Korean veterinary universities have the day-one competency to independently perform their professional duties.Methods: The Education Committee of the Korean Association of Veterinary Medical Colleges, composed of veterinary school professors and an experienced veterinarian in the clinic, reviewed domestic and international veterinary education-related materials to define basic clinical skills.Results: The Korean Veterinarian Entrustable Professional Activities (KVEPA) was introduced, followed by the subsequent development of 54 essential clinical skills based on the KVEPA.Conclusions and relevance: The veterinary basic clinical skills established through this study can be used as a specific guide for clinical education in Korean veterinary school, and is expected to play an important role in meeting the needs of the educational sector of the veterinary education accreditation standards.","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"25 5","pages":"e71"},"PeriodicalIF":1.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11450386/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142372211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alzheimer disease-like neuropathologic changes in a geriatric baboon (Papio hamadryas). 老年狒狒（Papio hamadryas）的阿尔茨海默病样神经病理变化。

IF 1.5 3区农林科学

Journal of Veterinary Science Pub Date : 2024-09-01 Epub Date: 2024-08-07 DOI: 10.4142/jvs.24080

William T Harrison, J Mark Cline, David L Caudell, Hillary F Huber, Carol A Shively, Thomas C Register, Suzanne Craft, Jason D Struthers

{"title":"Alzheimer disease-like neuropathologic changes in a geriatric baboon (Papio hamadryas).","authors":"William T Harrison, J Mark Cline, David L Caudell, Hillary F Huber, Carol A Shively, Thomas C Register, Suzanne Craft, Jason D Struthers","doi":"10.4142/jvs.24080","DOIUrl":"10.4142/jvs.24080","url":null,"abstract":"Importance: Alzheimer's disease (AD) is the most common cause of dementia in the elderly with the incidence rising exponentially after the age of 65 years. Unfortunately, effective treatments are extremely limited and definite diagnosis can only be made at autopsy. This is in part due to our limited understanding of the complex pathophysiology, including the various genetic, environmental, and metabolic contributing factors. In an effort to better understand this complex disease, researchers have employed nonhuman primates as translational models.Case presentation: This report aims to describe the AD-like neuropathology in the brain of a 37-year-old female baboon (Papio hamadryas), which at the time of her death made her the oldest hamadryas baboon at any member institution of the Association of Zoos and Aquariums. A diagnostic necropsy was performed, and the brain was evaluated for neurodegenerative disease. Frequent amyloid-β deposits were identified, consistent with what has been described in other geriatric nonhuman primates. Phospho-tau pathology, including neurofibrillary tangles, a feature not well-described in other primate models, was also abundant.Conclusions and relevance: Our results suggest that more detailed, prospective, longitudinal studies are warranted utilizing this particular species to see if they represent a viable model for human brain aging.","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":" ","pages":"e60"},"PeriodicalIF":1.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11450389/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of concurrent infection with Jaagsiekte sheep retrovirus and maedi-visna virus in China. 确定中国同时感染了Jaagsiekte羊逆转录病毒和maedi-visna病毒。

IF 1.5 3区农林科学

Journal of Veterinary Science Pub Date : 2024-09-01 Epub Date: 2024-08-08 DOI: 10.4142/jvs.24158

Xujie Duan, Xiaona Shi, Pei Zhang, Xiaoyue Du, Sixu Chen, Liang Zhang, Huiping Li, Yufei Zhang, Jinling Wang, Yulin Ding, Shuying Liu

{"title":"Identification of concurrent infection with Jaagsiekte sheep retrovirus and maedi-visna virus in China.","authors":"Xujie Duan, Xiaona Shi, Pei Zhang, Xiaoyue Du, Sixu Chen, Liang Zhang, Huiping Li, Yufei Zhang, Jinling Wang, Yulin Ding, Shuying Liu","doi":"10.4142/jvs.24158","DOIUrl":"10.4142/jvs.24158","url":null,"abstract":"Importance: Ovine pulmonary adenomatosis (OPA) and maedi-visna disease (MVD) are chronic and progressive infectious diseases in sheep caused by Jaagsiekte sheep retrovirus (JSRV) and maedi-visna virus (MVV), respectively.Objective: To investigate the pathological changes and conduct viral gene analysis of OPA and MVD co-occurrence in Inner Mongolia, China.Methods: Using gross pathology, histopathology, immunohistochemistry, ultrastructural pathology, PCR, and sequence analysis, we investigated the concurrent infection of JSRV and MVV in 319 Dorper rams slaughtered in a private slaughterhouse in Inner Mongolia, in 2022.Results: Of the 319 rams included, 3 showed concurrent JSRV and MVV infection. Gross lung pathology showed diffuse enlargement, consolidation, and greyish-white miliary nodules on the lung surface; the trachea was filled with a white foamy fluid; hilar and mediastinal lymph nodes were significantly enlarged. Histopathology results revealed typical OPA and MVD lesions in the lung tissue. Immunohistochemical results were positive for JSRV envelope protein (Env) in the tumor cells and MVV CA in alveolar macrophages. Transmission electron microscopy showed several virions and autophagosomes in the lung tissue, severely damaged mitochondria, and the induced mitophagy. Nucleotide sequences obtained for JSRV env and MVV gag showed the highest homology with the Inner Mongolian strains of JSRV env (JQ837489) and MVV gag (MW248464).Conclusions and relevance: Our study confirmed that OPA and MVD co-occurrence and identified the pathological changes in Inner Mongolia, China, thereby providing references for the identification of concurrent JSRV and MVV infections.","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":" ","pages":"e61"},"PeriodicalIF":1.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11450398/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of a new bovine picornavirus (Boosepivirus) in the Republic of Korea. 在大韩民国鉴定出一种新的牛皮卡病毒（Boosepivirus）。

IF 1.5 3区农林科学

Journal of Veterinary Science Pub Date : 2024-09-01 Epub Date: 2024-08-06 DOI: 10.4142/jvs.24148

Jeong-Byoung Chae, Seung-Uk Shin, Serim Kim, Hansong Chae, Won Gyeong Kim, Joon-Seok Chae, Hyuk Song, Jung-Won Kang

{"title":"Identification of a new bovine picornavirus (Boosepivirus) in the Republic of Korea.","authors":"Jeong-Byoung Chae, Seung-Uk Shin, Serim Kim, Hansong Chae, Won Gyeong Kim, Joon-Seok Chae, Hyuk Song, Jung-Won Kang","doi":"10.4142/jvs.24148","DOIUrl":"10.4142/jvs.24148","url":null,"abstract":"Importance: Despite advancements in herd management, feeding, and pharmaceutical interventions, neonatal calf diarrhea (NCD) remains a major global health concern. Bacteria, viruses, and parasites are the major contributors to NCD. Although several pathogens have been identified in the Republic of Korea (ROK), the etiological agents of numerous NCD cases have not been identified.Objective: To identify, for the first time, the prevalence and impact of Boosepivirus (BooV) on calf diarrhea in the ROK.Methods: Here, the unknown cause of calf diarrhea was determined using metagenomics We then explored the prevalence of certain pathogens, including BooV, that cause NCD. Seventy diarrheal fecal samples from Hanwoo (Bos taurus coreanae) calves were analyzed using reverse transcriptase and quantitative real-time polymerase chain reaction for pathogen detection and BooV isolate sequencing.Results: The complete genome of BooV was detected from unknown causes of calf diarrhea. And also, BooV was the most frequently detected pathogen (35.7%) among 8 pathogens in 70 diarrheic feces from Hanwoo calves. Co-infection analyses indicated that most BooV-positive samples were solely infected with BooV, indicating its significance in NCD in the ROK. All isolates were classified as BooV B in phylogenetic analysis.Conclusions and relevance: This is the first study to determine the prevalence and molecular characteristics of BooV in calf diarrhea in the ROK, highlighting the potential importance of BooV as a causative agent of calf diarrhea and highlighting the need for further research on its epidemiology and pathogenicity.","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":" ","pages":"e59"},"PeriodicalIF":1.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11450388/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142140456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lithium alleviates paralysis in experimental autoimmune neuritis in Lewis rats by modulating glycogen synthase kinase-3β activity. 锂通过调节糖原合酶激酶-3β的活性减轻路易斯大鼠实验性自身免疫性神经炎的麻痹症状

IF 1.5 3区农林科学

Journal of Veterinary Science Pub Date : 2024-09-01 DOI: 10.4142/jvs.24212

Meejung Ahn

{"title":"Lithium alleviates paralysis in experimental autoimmune neuritis in Lewis rats by modulating glycogen synthase kinase-3β activity.","authors":"Meejung Ahn","doi":"10.4142/jvs.24212","DOIUrl":"10.4142/jvs.24212","url":null,"abstract":"Importance: Guillain-Barré syndrome (GBS)-like neuropathy mimics the leading cause of sporadic acute nontraumatic limb paralysis in individuals from developed countries. Experimental autoimmune neuritis (EAN) is an animal model of GBS and of syndromes such as acute canine polyradiculoneuritis, seen in dogs and cats.Objective: The involvement of glycogen synthase kinase (GSK)-3β, a pro-inflammatory molecule, in rat EAN is not fully understood. This study evaluated the potential role of GSK-3β in EAN through its inhibition by lithium.Methods: Lewis rats were injected with SP26 antigen to induce EAN. Lithium was administered from 1 day before immunization to day 14 post-immunization (PI). Then the rats were euthanized and their neural tissues were prepared for histological and Western blotting analyses.Results: Lithium, an inhibitor of GSK-3, significantly ameliorated EAN paralysis in rats, when administered from day 1 to day 14 PI. This corresponded with reduced inflammation in the sciatic nerves of EAN rats, where phosphorylation of GSK-3β was also upregulated, indicating suppression of GSK-3.Conclusions and relevance: These findings suggest that lithium, an inhibitor of GSK-3β, plays a significant role in ameliorating rat EAN paralysis, by suppressing GSK-3β and its related signals in EAN-affected sciatic nerves.","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"25 5","pages":"e69"},"PeriodicalIF":1.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11450387/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142372214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gut microbial assessment among Hylobatidae at the National Wildlife Rescue Centre, Peninsular Malaysia. 马来西亚半岛国家野生动物救护中心对虹彩蝠科动物的肠道微生物进行评估。

IF 1.5 3区农林科学

Journal of Veterinary Science Pub Date : 2024-09-01 Epub Date: 2024-08-16 DOI: 10.4142/jvs.23312

Roberta Chaya Tawie Tingga, Millawati Gani, Abd Rahman Mohd-Ridwan, Nor Rahman Aifat, Ikki Matsuda, Badrul Munir Md-Zain

{"title":"Gut microbial assessment among Hylobatidae at the National Wildlife Rescue Centre, Peninsular Malaysia.","authors":"Roberta Chaya Tawie Tingga, Millawati Gani, Abd Rahman Mohd-Ridwan, Nor Rahman Aifat, Ikki Matsuda, Badrul Munir Md-Zain","doi":"10.4142/jvs.23312","DOIUrl":"10.4142/jvs.23312","url":null,"abstract":"Importance: Recent developments in genetic analytical techniques have enabled the comprehensive analysis of gastrointestinal symbiotic bacteria as a screening tool for animal health conditions, especially the endangered gibbons at the National Wildlife Rescue Centre (NWRC).Objective: High-throughput sequencing based on 16S ribosomal RNA genes was used to determine the baseline gut bacterial composition and identify potential pathogenic bacteria among three endangered gibbons housed in the NWRC.Methods: Feces were collected from 14 individuals (Hylobates lar, n = 9; Hylobates agilis, n = 4; and Symphalangus syndactylus, n = 1) from March to November 2022. Amplicon sequencing were conducted by targeting V3-V4 region.Results: The fecal microbial community of the study gibbons was dominated by Bacteroidetes and Firmicutes (phylum level), Prevotellaceae and Lachnospiraceae/Muribaculaceae (family level), and Prevotella (and its subgroups) (genera level). This trend suggests that the microbial community composition of the study gibbons differed insignificantly from previously reported conspecific or closely related gibbon species.Conclusions and relevance: This study showed no serious health problems that require immediate attention. However, relatively low alpha diversity and few potential bacteria related to gastrointestinal diseases and streptococcal infections were detected. Information on microbial composition is essential as a guideline to sustain a healthy gut condition of captive gibbons in NWRC, especially before releasing this primate back into the wild or semi-wild environment. Further enhanced husbandry environments in the NWRC are expected through continuous health monitoring and increase diversity of the gut microbiota through diet diversification.","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":" ","pages":"e65"},"PeriodicalIF":1.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11450390/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of symmetric dimethylarginine as a serologic marker for kidney function in striped skunks (Mephitis mephitis). 将对称二甲基精氨酸作为条纹鼬（Mephitis mephitis）肾功能血清学标记的研究。

IF 1.5 3区农林科学

Journal of Veterinary Science Pub Date : 2024-07-01 DOI: 10.4142/jvs.24057

Eun Jung, Soong-Hee Youn, Ki-Yong Shin, Hyeon-Joo Shin, Joon-Young Yang, Yeseul Yang, Jae-Ha Jung, Yongbaek Kim

{"title":"Investigation of symmetric dimethylarginine as a serologic marker for kidney function in striped skunks (Mephitis mephitis).","authors":"Eun Jung, Soong-Hee Youn, Ki-Yong Shin, Hyeon-Joo Shin, Joon-Young Yang, Yeseul Yang, Jae-Ha Jung, Yongbaek Kim","doi":"10.4142/jvs.24057","DOIUrl":"10.4142/jvs.24057","url":null,"abstract":"Importance: Kidney disease is prevalent among veterinary species, including zoo animals; however, investigations into this condition in striped skunks (Mephitis mephitis) are scarce. Diagnostic tools for kidney diseases in this species also remain limited.Objective: This study aimed to assess the utility of symmetric dimethylarginine as a biomarker for kidney disease in captive striped skunks in Korea.Methods: This retrospective study analysed 11 striped skunks housed at the Everland Zoo between 2017 and 2021. Blood samples were collected during health checks. Kidney function was assessed through blood analysis and diagnostic ultrasound, with necropsies conducted on deceased animals. Symmetric dimethylarginine levels were measured in 27 plasma samples collected from 11 skunks.Results: Over the study period, seven skunks were diagnosed with kidney disease. Analysis of 27 blood samples revealed a concurrent increase in SDMA levels with concentrations of blood urea nitrogen and blood creatinine. In 3 of the 7 skunks with kidney disease, symmetric dimethylarginine exceeded 14 µg/dL prior to the elevation of blood urea nitrogen and blood creatinine above the upper reference limit.Conclusions and relevance: To our knowledge, this is the first study investigating symmetric dimethylarginine in captive striped skunks in Korea. Our findings suggest that symmetric dimethylarginine may serve as an early and consistent biomarker for renal dysfunction in striped skunks. Further studies with larger clinical sample size from striped skunks are needed to validate the clinical utility of blood symmetric dimethylarginine concentration.","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"25 4","pages":"e52"},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11291430/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NOX4 and its association with myeloperoxidase and osteopontin in regulating endochondral ossification. NOX4 及其与髓过氧化物酶和骨素在调节软骨内骨化过程中的关联。

IF 1.5 3区农林科学

Journal of Veterinary Science Pub Date : 2024-07-01 Epub Date: 2024-05-24 DOI: 10.4142/jvs.24076

Kayoung Ko, Seohee Choi, Miri Jo, Chaeyoung Kim, Napissara Boonpraman, Jihyun Youm, Sun Shin Yi

{"title":"NOX4 and its association with myeloperoxidase and osteopontin in regulating endochondral ossification.","authors":"Kayoung Ko, Seohee Choi, Miri Jo, Chaeyoung Kim, Napissara Boonpraman, Jihyun Youm, Sun Shin Yi","doi":"10.4142/jvs.24076","DOIUrl":"10.4142/jvs.24076","url":null,"abstract":"Importance: Endochondral ossification plays an important role in skeletal development. Recent studies have suggested a link between increased intracellular reactive oxygen species (ROS) and skeletal disorders. Moreover, previous studies have revealed that increasing the levels of myeloperoxidase (MPO) and osteopontin (OPN) while inhibiting NADPH oxidase 4 (NOX4) can enhance bone growth. This investigation provides further evidence by showing a direct link between NOX4 and MPO, OPN in bone function.Objective: This study investigates NOX4, an enzyme producing hydrogen peroxide, in endochondral ossification and bone remodeling. NOX4's role in osteoblast formation and osteogenic signaling pathways is explored.Methods: Using NOX4-deficient (NOX4-/-) and ovariectomized (OVX) mice, we identify NOX4's potential mediators in bone maturation.Results: NOX4-/- mice displayed significant differences in bone mass and structure. Compared to the normal Control and OVX groups. Hematoxylin and eosin staining showed NOX4-/- mice had the highest trabecular bone volume, while OVX had the lowest. Proteomic analysis revealed significantly elevated MPO and OPN levels in bone marrow-derived cells in NOX4-/- mice. Immunohistochemistry confirmed increased MPO, OPN, and collagen II (COLII) near the epiphyseal plate. Collagen and chondrogenesis analysis supported enhanced bone development in NOX4-/- mice.Conclusions and relevance: Our results emphasize NOX4's significance in bone morphology, mesenchymal stem cell proteomics, immunohistochemistry, collagen levels, and chondrogenesis. NOX4 deficiency enhances bone development and endochondral ossification, potentially through increased MPO, OPN, and COLII expression. These findings suggest therapeutic implications for skeletal disorders.","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":" ","pages":"e49"},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11291435/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141442972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0