Whole genome sequencing analysis of enteropathogenic Escherichia coli from human and companion animals in Korea.

IF 1.5 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Veterinary Science Pub Date : 2025-01-01 Epub Date: 2024-12-02 DOI:10.4142/jvs.24225

Jae Young Oh, Kyung-Hyo Do, Jae Hong Jeong, SuMin Kwak, Sujin Choe, Dongheui An, Jong-Chan Chae, Kwangjun Lee, Kwang-Won Seo

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引用次数: 0

Abstract

Importance: This study is essential for comprehending the zoonotic transmission, antimicrobial resistance, and genetic diversity of enteropathogenic Escherichia coli (EPEC).

Objective: To improve our understanding of EPEC, this study focused on analyzing and comparing the genomic characteristics of EPEC isolates from humans and companion animals in Korea.

Methods: The whole genome of 26 EPEC isolates from patients with diarrhea and 20 EPEC isolates from companion animals in Korea were sequenced using the Illumina HiSeq X (Illumina, USA) and Oxford Nanopore MinION (Oxford Nanopore Technologies, UK) platforms.

Results: Most isolates were atypical EPEC, and did not harbor the bfpA gene. The most prevalent virulence genes were found to be ompT (humans: 61.5%; companion animals: 60.0%) followed by lpfA (humans: 46.2%; companion animals: 60.0%). Although pan-genome analyses showed no apparent correlation among the origin of the strains, virulence profiles, and antimicrobial resistance profiles, isolates included in clade A obtained from both humans and companion animals exhibited high similarity. Additionally, all the isolates included in clade A encoded the ompT gene and did not encode the hlyE gene. The two isolates from companion animals harbored an incomplete bundle-forming pilus region encoding bfpA and bfpB. Moreover, the type IV secretion system-associated genes tra and trb were found in the bfpA-encoding isolates from humans.

Conclusions and relevance: Whole-genome sequencing enabled a more accurate analysis of the phylogenetic structure of EPEC and provided better insights into the understanding of EPEC epidemiology and pathogenicity.

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韩国人及伴侣动物肠致病性大肠杆菌全基因组测序分析。

重要性：本研究对了解肠致病性大肠杆菌（EPEC）的人畜共患传播、抗菌素耐药性和遗传多样性至关重要。目的：分析比较韩国人与伴侣动物分离株EPEC的基因组特征，以提高对EPEC的认识。方法：采用Illumina HiSeq X （Illumina， USA）和Oxford Nanopore MinION （Oxford Nanopore Technologies， UK）测序平台，对韩国腹泻患者分离的26株EPEC和伴侣动物分离的20株EPEC进行全基因组测序。结果：多数分离株为非典型EPEC，不含bfpA基因。发现最普遍的毒力基因是ompT(人类：61.5%；伴侣动物：60.0%)，其次是lpfA(人类：46.2%；伴侣动物：60.0%)。尽管泛基因组分析显示菌株的起源、毒力谱和抗微生物药物耐药性谱之间没有明显的相关性，但从人类和伴侣动物中获得的A枝分离株表现出高度的相似性。此外，A支系的所有分离株都编码ompT基因，而不编码hlyE基因。来自伴侣动物的两个分离株含有一个编码bfpA和bfpB的不完整的束形成毛区。此外，在编码bfpa的人类分离株中发现了IV型分泌系统相关基因tra和trb。结论和相关性：全基因组测序能够更准确地分析EPEC的系统发育结构，并为了解EPEC的流行病学和致病性提供更好的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Veterinary Science 农林科学-兽医学

CiteScore

3.10

自引率

5.60%

发文量

审稿时长

1.3 months

期刊介绍： The Journal of Veterinary Science (J Vet Sci) is devoted to the advancement and dissemination of scientific knowledge concerning veterinary sciences and related academic disciplines. It is an international journal indexed in the Thomson Scientific Web of Science, SCI-EXPANDED, Sci Search, BIOSIS Previews, Biological Abstracts, Focus on: Veterinary Science & Medicine, Zoological Record, PubMed /MEDLINE, Index Medicus, Pubmed Central, CAB Abstracts / Index Veterinarius, EBSCO, AGRIS and AGRICOLA. This journal published in English by the Korean Society of Veterinary Science (KSVS) being distributed worldwide.