Journal of Veterinary Science最新文献

{"title":"Development of visual dye-based loop-mediated isothermal amplification assays for <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> detection in ruminants.","authors":"Sudu Hakuruge Madusha Pramud Wimalasena, Sang Young Seo, Han Gyu Lee, Young Hun Jung, Yoon Jung Do, Ara Cho, Changyong Choe, Are Sun Yoo, Seogjin Kang, Jun Ho Lee, Han Sang Yoo, Eun-Yeong Bok","doi":"10.4142/jvs.24201","DOIUrl":"10.4142/jvs.24201","url":null,"abstract":"<p><strong>Importance: </strong><i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> (MAP), the causative agent of Johne's disease (JD) in ruminants, is a potential zoogenic pathogen. At the asymptomatic subclinical stage, infected animals periodically shed the pathogen through feces and milk, serving as silent carriers for disease transmission. In Republic of Korea, where treatment for JD is not pursued and culling is the primary management strategy for infected animals, early detection is crucial for effective disease control.</p><p><strong>Objective: </strong>In this study, we aimed to develop a new primer set for the loop-mediated isothermal amplification (LAMP) method for facilitating visual-based detection of MAP.</p><p><strong>Methods: </strong>We designed LAMP primers for the MAP-specific gene IS<i>1311</i> and optimized the reaction conditions to enhance diagnostic sensitivity and specificity. Practical application was evaluated using 170 field samples of feces and intestinal tissues from cattle and goats.</p><p><strong>Results: </strong>The LAMP method demonstrated sensitivity superior to that of the conventional polymerase chain reaction (PCR) method and equal to that of real-time-PCR targeting the F<i>57</i> gene, detecting as low as 0.1 pg MAP DNA per reaction. Notably, we integrated a mixed dye composed of calcein and hydroxynaphthol blue at an optimal ratio, enabling clear visual distinction of results under natural light. In practical application, the LAMP assay demonstrated a true positive rate of 100% and true-negative rate of 92.22%.</p><p><strong>Conclusions and relevance: </strong>The developed mixed dye-LAMP method facilitates immediate, sensitive diagnosis by providing a user-friendly readout. By reducing diagnostic time and enhancing visual clarity without specialized equipment, it significantly improves JD control in the livestock industry.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 3","pages":"e31"},"PeriodicalIF":1.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146026/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of tissue tropism of chicken anaemia virus on chicken embryos: a molecular insight.","authors":"Sedeneinuo Suohu, Gurusamipalayam Amirthalingam Balasubramaniam, Ayyasamy Arulmozhi, Thippichettipalayam Ramasamy Gopalakrishnamurthy, Angamuthu Raja","doi":"10.4142/jvs.24186","DOIUrl":"10.4142/jvs.24186","url":null,"abstract":"<p><strong>Importance: </strong>Few studies have examined the interactions of the chicken anemia virus (CAV) with the immune system of embryos.</p><p><strong>Objective: </strong>To study the pathogenicity of the CAV in chicken embryos with respect to its tissue tropism and viral load in different tissues.</p><p><strong>Method: </strong>The pathogenicity of the CAV was examined in about-to-hatch embryos through cytology, histopathological examinations, molecular detection, immunocytochemistry (ICC), immunofluorescent test, and immunohistochemistry techniques.</p><p><strong>Results: </strong>The experimental study using a polymerase chain reaction (PCR) assay revealed the presence of CAV in the liver, bone marrow, and thymus tissues of embryos where embryos were inoculated with CAV-positive bone marrow specimens obtained from chicken anemia-suspect cases. Intracytoplasmic and intranuclear inclusion bodies were detected cytologically in the bone marrow of the embryos, whereas no inclusion body was detected in the thymus. On the other hand, both kinds of inclusion bodies were observed in bone marrow and thymus in the histopathology. In addition, various organs of PCR-positive CAV embryos revealed the depletion of lymphocytes and hematopoietic cells in the thymus and bone marrow, respectively. The CAV antigen was confirmed in the bone marrow and thymus cytological smears using ICC and immunofluorescence techniques.</p><p><strong>Conclusions and relevance: </strong>Viral antigen exhibited more tropism towards the bone marrow of embryos than the thymus in ICC and immunofluorescence techniques. The presence of CD44, a marker for progenitor cells and an antigen in the serial sections of bone marrow, confirmed the relevance of viral tropism for the hemocytoblasts of the bone marrow as the main target cells in embryos and subsequently in chicks for viral replication.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 3","pages":"e17"},"PeriodicalIF":1.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146021/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Baicalin protects neurons from oxidative stress and apoptosis induced by glutamate excitotoxicity in HT-22 cells.","authors":"Hyun-Kyoung Son, Phil-Ok Koh","doi":"10.4142/jvs.24334","DOIUrl":"10.4142/jvs.24334","url":null,"abstract":"<p><strong>Importance: </strong>Baicalin is a flavonoid compound with various functions, including neuroprotective effects. Glutamate is an essential excitatory neurotransmitter involved in synaptic transmission, but it can also induce neuronal damage through excitotoxicity.</p><p><strong>Objective: </strong>To analyze the anti-oxidant and anti-apoptotic effects of baicalin on glutamate-exposed neuronal cells.</p><p><strong>Methods: </strong>Mouse neuronal hippocampal HT-22 cells were cultured and treated with glutamate (5 mM) and/or baicalin (10, 30, 50 µM). Baicalin was administered 1 h before glutamate treatment, and cells were collected 24 h following glutamate exposure. Reactive oxygen species (ROS) and lipid peroxidation (LPO) analyses were performed to determine the oxidative stress. Western blot and immunocytochemical staining were performed to investigate the expressions of bcl-2, bax, and caspase-3.</p><p><strong>Results: </strong>Glutamate induced severe neuronal damage, including cell morphological condensation, which was attenuated by baicalin treatment. Baicalin treatment ameliorated the decrease in cell viability due to glutamate toxicity. Baicalin mitigated glutamate-induced increase of ROS and LPO in a dose-dependent manner. Glutamate exposure induced the downregulation of bcl-2 and the upregulation of bax, thereby reducing the bcl-2 to bax ratio, while administration of baicalin prevented these changes. Baicalin treatment ameliorated the glutamate toxicity-induced increase in caspase-3.</p><p><strong>Conclusions and relevance: </strong>Baicalin exerts antioxidant and anti-apoptotic functions against glutamate toxicity in neurons by preventing oxidative stress and inhibiting the apoptotic pathway.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 3","pages":"e29"},"PeriodicalIF":1.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146029/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Imported case of canine viscerocutaneous leishmaniasis in South Korea: clinical presentation and diagnostic approach in a Labrador Retriever.","authors":"Ki-Yeon Son, Gyeong-Gook Park, Joong-Hyun Song","doi":"10.4142/jvs.24296","DOIUrl":"10.4142/jvs.24296","url":null,"abstract":"<p><strong>Importance: </strong>Leishmaniasis, a sandfly-borne disease, can infect both humans and dogs, with dogs acting as key reservoirs. This report documents the first case of leishmaniasis in South Korea, identified in a dog imported from Spain. It highlights the importance for early detection and careful monitoring of dogs imported from endemic regions to prevent the introduction and spread of leishmaniasis to regions such as South Korea, where the disease is rare.</p><p><strong>Case presentation: </strong>A 2-year-old male Labrador Retriever dog, imported from Spain at 12 months old, was presented with a 5-month history of generalized cutaneous lesions and a 1-month history of chronic diarrhea. Microscopic examination of peripheral blood smears revealed amastigote-infected macrophages and whole-blood polymerase chain reaction confirmed a diagnosis of viscerocutaneous leishmaniasis. The dog was treated with allopurinol, the only available treatment for leishmaniasis in South Korea, alongside supportive management. However, the patient showed a poor response to treatment.</p><p><strong>Conclusions and relevance: </strong>This case of canine leishmaniasis in South Korea highlights the growing risk of imported infections in non-endemic areas and underscores the need for greater awareness and understanding of the clinical features of Leishmaniasis for timely diagnosis and management in dogs with a history of travel or adoption from endemic regions.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 3","pages":"e33"},"PeriodicalIF":1.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146019/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safety and efficacy of a <i>Bordetella bronchiseptica</i> outer membrane proteins (OMPs) subunit vaccine in a murine model.","authors":"Ji Young Jang, Byung Joon Park, Huiyeon Song, Seungshik Rha, Hyoung Joon Moon, Han Sang Yoo","doi":"10.4142/jvs.25018","DOIUrl":"10.4142/jvs.25018","url":null,"abstract":"<p><strong>Importance: </strong><i>Bordetella bronchiseptica</i> is a major pathogen responsible for respiratory infections in dogs, posing significant challenges to companion animal health. The whole-cell vaccine currently available has various adverse effects, and concerns persist regarding the efficacy of acellular vaccines. Therefore, developing a safer and more effective vaccine is critical for addressing these issues.</p><p><strong>Objective: </strong>This study evaluated the safety and efficacy of <i>B. bronchiseptica</i> outer membrane proteins (OMPs) and dermonecrotic toxin (DNT) as subunit vaccine candidates, individually and in combination, compared to conventional vaccine formulations in murine models.</p><p><strong>Methods: </strong>The immune responses and side effects were assessed after vaccination with OMPs and DNT as subunit vaccine candidates, individually and in combination. The safety evaluations included monitoring the body temperature, weight, and vitality scores, while the efficacy was measured using immunoglobulin quantification, hemagglutination-inhibition (HI) assays, and challenge tests. The vaccine candidates were compared with conventional vaccine formulations.</p><p><strong>Results: </strong>The OMP-based vaccine induced a T helper 1-biased immune response, providing robust protection against <i>B. bronchiseptica</i> with minimal adverse effects. More than 90% disease protection was achieved at OMP concentrations of 25-50 µg/mL, with immunoglobulin G levels exceeding 2¹³ (8,192) and HI titers surpassing 2^7.5 (181). In contrast, commercially available Bordetella vaccine formulations, which contain cellular wall components and toxins, showed reduced efficacy and heightened side effects.</p><p><strong>Conclusions and relevance: </strong>OMPs are a promising, cost-effective component for <i>B. bronchiseptica</i> vaccine development, providing a balance between safety and efficacy. These insights could promote the development of improved Bordetella vaccines for dogs and potentially humans, addressing the longstanding trade-offs in current vaccine formulations and contributing to better animal health.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 3","pages":"e39"},"PeriodicalIF":1.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146018/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methodological evaluation of ultrasound-guided modified traumatic catheterization in dogs with suspected urothelial carcinoma.","authors":"Jisu Lee, Ul Soo Choi, Minji Kwon, Hakyoung Yoon","doi":"10.4142/jvs.24308","DOIUrl":"10.4142/jvs.24308","url":null,"abstract":"<p><strong>Importance: </strong>Bladder tumors in dogs, particularly urothelial carcinoma (UC), pose a diagnostic challenge due to overlapping imaging features with benign conditions. Accurate diagnosis is crucial for appropriate treatment and prognosis.</p><p><strong>Objective: </strong>To assess the effectiveness of a modified traumatic catheterization technique using a 4-hole catheter for cytological sampling in dogs with bladder masses.</p><p><strong>Methods: </strong>Fifteen dogs with hematuria and mass-like bladder lesions identified using ultrasonography were included in the study. 15 dogs underwent modified traumatic catheterization using a four-hole catheter created by adding two extra holes to a 6 or 8 Fr feeding tube. The catheter was inserted into the bladder, and cells were aspirated from the lesion under ultrasound guidance. The procedure's efficacy was evaluated both quantitatively and qualitatively.</p><p><strong>Results: </strong>All 15 cases achieved a 100% cell acquisition rate with high cellularity. The median visualization score was 3/3, the manipulation score was 3/3, and the cellularity score was 2/2. The average procedure time was 8.8 min. Diagnoses included 12 cases of UC and three cases of polypoid cystitis/urinary bladder polyps. No significant adverse effects, including bladder perforation or urethral trauma, were observed. Mild bleeding occurred in some cases but resolved spontaneously.</p><p><strong>Conclusions and relevance: </strong>Modified traumatic catheterization using a 4-hole catheter is a safe, efficient, and minimally invasive technique for diagnosing UC and other bladder conditions. It is particularly useful for small-sized dogs and those unsuitable for invasive procedures. The enhanced catheter design improves lesion contact and sampling efficiency by offering a valuable tool for veterinary practitioners.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 3","pages":"e32"},"PeriodicalIF":1.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146027/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perioperative use of flash glucose monitoring system in dogs undergoing general surgery: a clinical feasibility study.","authors":"Seung-Yeon Yu, Hakhyun Kim, Min Jang, Sungin Lee","doi":"10.4142/jvs.24271","DOIUrl":"10.4142/jvs.24271","url":null,"abstract":"<p><strong>Importance: </strong>Flash Glucose Monitoring System (FGMS; FreeStyle Libre) enables non-invasive, continuous glucose monitoring. However, its perioperative utility in canine surgeries has not been evaluated. This study highlights the feasibility of FGMS in perioperative glucose management for veterinary patients.</p><p><strong>Objective: </strong>This study aimed to evaluate the clinical applicability and accuracy of FGMS in perioperative glucose monitoring in dogs.</p><p><strong>Methods: </strong>Thirty-two dogs that underwent surgery were monitored during the preoperative, intraoperative, and postoperative phases using FGMS and portable blood glucose meter (PBGM).</p><p><strong>Results: </strong>A total of 224 paired glucose measurements were analyzed for correlation and accuracy using ISO 15197:2013 standards. Spearman's correlation analysis revealed positive correlation between FGMS and PBGM (ρ = 0.894, ρ = 0.823, and ρ = 0.795, respectively) during the preoperative, intraoperative, and postoperative phases, respectively (<i>p</i> < 0.001). The Bland-Altman plot of the difference between FGMS and PBGM showed a positive bias during the intraoperative phase (23.3 mg/dL), despite a negative bias in the preoperative (-16.0 mg/dL) and postoperative (-4.58 mg/dL) phases. Parkes consensus error grid analysis demonstrated that 100% of the data pairs fell within clinically acceptable zones (zones A and B) throughout the operation.</p><p><strong>Conclusions and relevance: </strong>FGMS provides continuous and noninvasive glucose monitoring in dogs with clinical feasibility throughout the perioperative period, although its accuracy decreases during the intraoperative phase. For patients with relatively stable blood glucose levels, the Libre device is clinically effective for perioperative glucose monitoring and provides a simpler and more accessible alternative to traditional methods.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 3","pages":"e23"},"PeriodicalIF":1.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146024/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphology of subclavian artery branching patterns in cats (<i>Felis catus</i>).","authors":"Young-Jin Jang, Pill Soon Jeong, Hyun-Jin Tae, Byung-Yong Park, In-Shik Kim, Dongchoon Ahn","doi":"10.4142/jvs.25014","DOIUrl":"10.4142/jvs.25014","url":null,"abstract":"<p><strong>Importance: </strong>The subclavian artery (SB) is a crucial vascular structure that supplies the neck, thoracic wall, and forelimb. In carnivores, SB branching patterns exhibit considerable interspecific and intraspecific variation. Despite the increasing recognition of cats as companion animals, detailed studies of feline SB branching patterns remain limited.</p><p><strong>Objective: </strong>We aimed to investigate the SB branching patterns in cats, analyze intraspecific variations, and compare these findings with those observed in other carnivores.</p><p><strong>Methods: </strong>Silicone casts of the SBs were prepared from 35 cats (15 males and 20 females). Branching locations of arterial branches were measured as the distance from the aortic arch to each branching point. The SB branching patterns were analyzed based on the branching relationships among the four major branches: the vertebral artery (VT), costocervical trunk (CCT), superficial cervical artery (SC), and internal thoracic artery (IT).</p><p><strong>Results: </strong>Various branching patterns were observed in the SB with a total of 33 branching types. The main branching patterns were determined to be VT, followed by IT, CCT, and SC. The right SB branches exhibited more distal branching locations from the aortic arch compared to the left SB branches. Across individuals, the VT, CCT, and SC followed a consistent order, whereas the IT varied in position.</p><p><strong>Conclusions and relevance: </strong>This study offers a detailed analysis of the SB branching patterns in cats, highlighting significant intraspecific variation and distinctive branching order. These findings enhance our understanding of vascular diversity among carnivores and provide a strong foundation for future comparative anatomical and developmental investigations.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 3","pages":"e35"},"PeriodicalIF":1.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146025/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological investigation and fiber gene genetic analysis of fowl adenovirus serotype 4 from 2017 to 2021 in Yunnan Province, China.","authors":"Xiaoyan Xue, Zhenxing Zhang, Qinhong Yang, Wei Wang, Jianling Song","doi":"10.4142/jvs.24121","DOIUrl":"10.4142/jvs.24121","url":null,"abstract":"<p><strong>Importance: </strong>Through the epidemiological survey of fowl adenovirus serotype 4 (FAdV-4) in poultry and wild birds in Yunnan Province from 2017 to 2021, this study provides a scientific basis for the prevention and control of FAdV-4 in the region. Currently, there is a lack of monitoring and epidemiological research on FAdV-4 in wild birds in China, offering valuable insights for future studies on the virus in wild populations. Additionally, the analysis of the fiber gene sequence of FAdV-4 identified the prevalent strains in Yunnan Province, which lays the groundwork for future treatment and prevention strategies.</p><p><strong>Objective: </strong>This study aimed to understand the epidemic situation of FAdV-4 in Yunnan Province and provide a reference for the prevention and control of the infection.</p><p><strong>Methods: </strong>Suspected samples of FAdV-4 were collected with the wild birds from overwintering habitat and naturally dying poultry from poultry farms in Yunnan Province, and polymerase chain reaction (PCR) detection of FAdV-4 and related co-infection pathogens, pathogen isolation, and genetic evolution analysis of the <i>fiber</i> gene were carried out.</p><p><strong>Results: </strong>The results showed that the FAdV-4 positive rate in poultry and migratory birds was 11.3% and 0.3%, respectively. The infection rate of the poultry tissue samples was high in central and western Yunnan Province. The detection of common poultry infectious diseases prone to co-infection, it was found that the birds were mainly co-infected with pathogens such as infectious laryngotracheitis, chicken infectious anemia virus, and avian influenza virus. Five poultry-source strains and one wild bird-source strain were isolated, and phylogenetic analysis showed that they all had high homology with the early Chinese highly pathogenic strains and clustered with the same genetic and evolutionary clade as the Chinese reference strain. The fiber protein is characterized by loci that indicate highly pathogenic strains (G219D, P307A, V319I, and A308T).</p><p><strong>Conclusions and relevance: </strong>The results of our study demonstrate that FAdV-4 continues to exhibit a high prevalence rate among poultry populations in Yunnan Province, China. Notably, we report the first detection of FAdV-4 in the feces of black-necked cranes.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 3","pages":"e27"},"PeriodicalIF":1.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146020/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel thrombospondin-1 variant as a potential diagnostic biomarker and therapeutic target in canine mammary tumor and osteosarcoma cells.","authors":"Yukyung Hong, Heyeon Baik, Jeongmin Lee, Won Seok Oh, Junsun Ryu, Seung Joon Baek","doi":"10.4142/jvs.24165","DOIUrl":"10.4142/jvs.24165","url":null,"abstract":"<p><strong>Importance: </strong>Thrombospondin-1 (TSP1) is a vital glycoprotein that plays a key role in critical biological functions, including cell migration, invasion, angiogenesis, and proliferation. Understanding the roles of its variants, particularly TSP1 variant (TSP1V), is critical for cancer biology and therapy.</p><p><strong>Objective: </strong>This study examined the expression of the transcriptional variant TSP1V, focusing on canine TSP1 sequences.</p><p><strong>Methods: </strong>The expression of canine TSP1 sequences was analyzed using a reverse transcription polymerase chain reaction (RT-PCR) to identify the <i>TSP1</i> wild-type (<i>dTSP1W</i>) and novel canine <i>TSP1V</i> transcripts (<i>dTSP1V</i>). The effects of damnacanthal and genistein, anticancer compounds, on the viability of canine mammary and osteosarcoma cell lines were assessed by modulating the expression ratios of <i>TSP1V</i> to <i>TSP1W</i> at the transcriptional level. RT-PCR analysis compared the relative <i>dTSP1V</i> and <i>dTSP1W</i> concentrations in normal and tumor canine mammary tissues.</p><p><strong>Results: </strong>Two <i>dTSP1V</i> were identified. Treatment with damnacanthal and genistein decreased cell proliferation in canine mammary and osteosarcoma cell lines, associated with changes in the <i>TSP1V</i> to <i>TSP1W</i> expression ratio. RT-PCR analysis revealed increased <i>dTSP1V</i> expression in normal tissues, while <i>dTSP1W</i> expression was elevated in tumor tissues.</p><p><strong>Conclusions and relevance: </strong>TSP1V may be a potential diagnostic biomarker and therapeutic target for mammary tumors and osteosarcoma in dogs. The differential expression of <i>dTSP1V</i> and <i>dTSP1W</i> in normal versus tumor tissues underscores the importance of TSPIV in cancer biology, expanding the understanding of its role beyond human thyroid cancer and laying the groundwork for future research in other cancers and species.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 2","pages":"e22"},"PeriodicalIF":1.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11972944/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}