A novel thrombospondin-1 variant as a potential diagnostic biomarker and therapeutic target in canine mammary tumor and osteosarcoma cells.

IF 1.5 3区 农林科学 Q2 VETERINARY SCIENCES
Yukyung Hong, Heyeon Baik, Jeongmin Lee, Won Seok Oh, Junsun Ryu, Seung Joon Baek
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引用次数: 0

Abstract

Importance: Thrombospondin-1 (TSP1) is a vital glycoprotein that plays a key role in critical biological functions, including cell migration, invasion, angiogenesis, and proliferation. Understanding the roles of its variants, particularly TSP1 variant (TSP1V), is critical for cancer biology and therapy.

Objective: This study examined the expression of the transcriptional variant TSP1V, focusing on canine TSP1 sequences.

Methods: The expression of canine TSP1 sequences was analyzed using a reverse transcription polymerase chain reaction (RT-PCR) to identify the TSP1 wild-type (dTSP1W) and novel canine TSP1V transcripts (dTSP1V). The effects of damnacanthal and genistein, anticancer compounds, on the viability of canine mammary and osteosarcoma cell lines were assessed by modulating the expression ratios of TSP1V to TSP1W at the transcriptional level. RT-PCR analysis compared the relative dTSP1V and dTSP1W concentrations in normal and tumor canine mammary tissues.

Results: Two dTSP1V were identified. Treatment with damnacanthal and genistein decreased cell proliferation in canine mammary and osteosarcoma cell lines, associated with changes in the TSP1V to TSP1W expression ratio. RT-PCR analysis revealed increased dTSP1V expression in normal tissues, while dTSP1W expression was elevated in tumor tissues.

Conclusions and relevance: TSP1V may be a potential diagnostic biomarker and therapeutic target for mammary tumors and osteosarcoma in dogs. The differential expression of dTSP1V and dTSP1W in normal versus tumor tissues underscores the importance of TSPIV in cancer biology, expanding the understanding of its role beyond human thyroid cancer and laying the groundwork for future research in other cancers and species.

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来源期刊
Journal of Veterinary Science
Journal of Veterinary Science 农林科学-兽医学
CiteScore
3.10
自引率
5.60%
发文量
86
审稿时长
1.3 months
期刊介绍: The Journal of Veterinary Science (J Vet Sci) is devoted to the advancement and dissemination of scientific knowledge concerning veterinary sciences and related academic disciplines. It is an international journal indexed in the Thomson Scientific Web of Science, SCI-EXPANDED, Sci Search, BIOSIS Previews, Biological Abstracts, Focus on: Veterinary Science & Medicine, Zoological Record, PubMed /MEDLINE, Index Medicus, Pubmed Central, CAB Abstracts / Index Veterinarius, EBSCO, AGRIS and AGRICOLA. This journal published in English by the Korean Society of Veterinary Science (KSVS) being distributed worldwide.
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