A novel thrombospondin-1 variant as a potential diagnostic biomarker and therapeutic target in canine mammary tumor and osteosarcoma cells.

IF 1.5 3区 农林科学 Q2 VETERINARY SCIENCES
Yukyung Hong, Heyeon Baik, Jeongmin Lee, Won Seok Oh, Junsun Ryu, Seung Joon Baek
{"title":"A novel thrombospondin-1 variant as a potential diagnostic biomarker and therapeutic target in canine mammary tumor and osteosarcoma cells.","authors":"Yukyung Hong, Heyeon Baik, Jeongmin Lee, Won Seok Oh, Junsun Ryu, Seung Joon Baek","doi":"10.4142/jvs.24165","DOIUrl":null,"url":null,"abstract":"<p><strong>Importance: </strong>Thrombospondin-1 (TSP1) is a vital glycoprotein that plays a key role in critical biological functions, including cell migration, invasion, angiogenesis, and proliferation. Understanding the roles of its variants, particularly TSP1 variant (TSP1V), is critical for cancer biology and therapy.</p><p><strong>Objective: </strong>This study examined the expression of the transcriptional variant TSP1V, focusing on canine TSP1 sequences.</p><p><strong>Methods: </strong>The expression of canine TSP1 sequences was analyzed using a reverse transcription polymerase chain reaction (RT-PCR) to identify the <i>TSP1</i> wild-type (<i>dTSP1W</i>) and novel canine <i>TSP1V</i> transcripts (<i>dTSP1V</i>). The effects of damnacanthal and genistein, anticancer compounds, on the viability of canine mammary and osteosarcoma cell lines were assessed by modulating the expression ratios of <i>TSP1V</i> to <i>TSP1W</i> at the transcriptional level. RT-PCR analysis compared the relative <i>dTSP1V</i> and <i>dTSP1W</i> concentrations in normal and tumor canine mammary tissues.</p><p><strong>Results: </strong>Two <i>dTSP1V</i> were identified. Treatment with damnacanthal and genistein decreased cell proliferation in canine mammary and osteosarcoma cell lines, associated with changes in the <i>TSP1V</i> to <i>TSP1W</i> expression ratio. RT-PCR analysis revealed increased <i>dTSP1V</i> expression in normal tissues, while <i>dTSP1W</i> expression was elevated in tumor tissues.</p><p><strong>Conclusions and relevance: </strong>TSP1V may be a potential diagnostic biomarker and therapeutic target for mammary tumors and osteosarcoma in dogs. The differential expression of <i>dTSP1V</i> and <i>dTSP1W</i> in normal versus tumor tissues underscores the importance of TSPIV in cancer biology, expanding the understanding of its role beyond human thyroid cancer and laying the groundwork for future research in other cancers and species.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"26 2","pages":"e22"},"PeriodicalIF":1.5000,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11972944/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Veterinary Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.4142/jvs.24165","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Importance: Thrombospondin-1 (TSP1) is a vital glycoprotein that plays a key role in critical biological functions, including cell migration, invasion, angiogenesis, and proliferation. Understanding the roles of its variants, particularly TSP1 variant (TSP1V), is critical for cancer biology and therapy.

Objective: This study examined the expression of the transcriptional variant TSP1V, focusing on canine TSP1 sequences.

Methods: The expression of canine TSP1 sequences was analyzed using a reverse transcription polymerase chain reaction (RT-PCR) to identify the TSP1 wild-type (dTSP1W) and novel canine TSP1V transcripts (dTSP1V). The effects of damnacanthal and genistein, anticancer compounds, on the viability of canine mammary and osteosarcoma cell lines were assessed by modulating the expression ratios of TSP1V to TSP1W at the transcriptional level. RT-PCR analysis compared the relative dTSP1V and dTSP1W concentrations in normal and tumor canine mammary tissues.

Results: Two dTSP1V were identified. Treatment with damnacanthal and genistein decreased cell proliferation in canine mammary and osteosarcoma cell lines, associated with changes in the TSP1V to TSP1W expression ratio. RT-PCR analysis revealed increased dTSP1V expression in normal tissues, while dTSP1W expression was elevated in tumor tissues.

Conclusions and relevance: TSP1V may be a potential diagnostic biomarker and therapeutic target for mammary tumors and osteosarcoma in dogs. The differential expression of dTSP1V and dTSP1W in normal versus tumor tissues underscores the importance of TSPIV in cancer biology, expanding the understanding of its role beyond human thyroid cancer and laying the groundwork for future research in other cancers and species.

一种新的血小板反应蛋白-1变异作为犬乳腺肿瘤和骨肉瘤细胞的潜在诊断生物标志物和治疗靶点。
重要性:血小板反应蛋白-1 (TSP1)是一种重要的糖蛋白,在关键的生物学功能中起关键作用,包括细胞迁移、侵袭、血管生成和增殖。了解其变体,特别是TSP1变体(TSP1V)的作用对癌症生物学和治疗至关重要。目的:研究犬TSP1V转录变异的表达,重点研究犬TSP1序列。方法:采用逆转录聚合酶链反应(RT-PCR)分析犬TSP1序列的表达,鉴定TSP1野生型(dTSP1W)和新型犬TSP1V转录本(dTSP1V)。通过在转录水平上调节TSP1V与TSP1W的表达比例,研究了抗癌化合物丹那坎达尔和染料木素对犬乳腺和骨肉瘤细胞系生存能力的影响。RT-PCR分析比较了正常和肿瘤犬乳腺组织中dTSP1V和dTSP1W的相对浓度。结果:鉴定出2株dTSP1V。damnacanthal和染料木素治疗可以降低犬乳腺和骨肉瘤细胞系的细胞增殖,这与TSP1V与TSP1W表达比的变化有关。RT-PCR分析显示,dTSP1V在正常组织中表达升高,而dTSP1W在肿瘤组织中表达升高。结论及意义:TSP1V可能是犬乳腺肿瘤和骨肉瘤的潜在诊断生物标志物和治疗靶点。dTSP1V和dTSP1W在正常和肿瘤组织中的差异表达强调了TSPIV在癌症生物学中的重要性,扩大了对其在人类甲状腺癌之外的作用的理解,并为未来在其他癌症和物种中的研究奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Journal of Veterinary Science
Journal of Veterinary Science 农林科学-兽医学
CiteScore
3.10
自引率
5.60%
发文量
86
审稿时长
1.3 months
期刊介绍: The Journal of Veterinary Science (J Vet Sci) is devoted to the advancement and dissemination of scientific knowledge concerning veterinary sciences and related academic disciplines. It is an international journal indexed in the Thomson Scientific Web of Science, SCI-EXPANDED, Sci Search, BIOSIS Previews, Biological Abstracts, Focus on: Veterinary Science & Medicine, Zoological Record, PubMed /MEDLINE, Index Medicus, Pubmed Central, CAB Abstracts / Index Veterinarius, EBSCO, AGRIS and AGRICOLA. This journal published in English by the Korean Society of Veterinary Science (KSVS) being distributed worldwide.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信