Hongyu Zhong, Kerong Li, Min Li, Kun Zhang, Yi Zhang, Luqi Liang, Haifeng Liu, Zhijun Zhong, Guangneng Peng, Xiaoxiao Zhou, Ziyao Zhou
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Detection and genetic evolution analysis of porcine reproductive and respiratory syndrome virus in Chengdu from January 2021 to December 2022.
Importance: Pandemics involving the porcine reproductive and respiratory syndrome virus (PRRSV) impact swine health and productivity, causing economic devastation, especially in large-scale feeding areas.
Objective: This study examined the prevalence and genetic variations of PRRSV in Chengdu, China, a vital transportation hub and the main area of pig breeding in southwest China, between January 2021 and December 2022.
Methods: The samples were collected from 13 key pig-feeding areas in Chengdu. The positive PRRSV samples were detected using a real-time quantitative polymerase chain reaction. The genetic variations of the PRRSV were analyzed by amplifying the open reading frame 5 (ORF5) gene and comparing it with relevant reference sequences.
Results: The PRRSV positive rate in Chengdu was 11.42% (276/2,416), including sublines 8.7, 5.1, 3.0, and 1.8. The ORF5 gene sequences in the samples had no insertion or deletion of amino acids but widespread variations, including the 13th and 151st positions, which were associated with viral virulence.
Conclusions and relevance: The PRRSV pandemic in Chengdu was relevant, and recombination between vaccine strains and field strains can contribute to vaccine immune failure.
期刊介绍：
The Journal of Veterinary Science (J Vet Sci) is devoted to the advancement and dissemination of scientific knowledge concerning veterinary sciences and related academic disciplines. It is an international journal indexed in the Thomson Scientific Web of Science, SCI-EXPANDED, Sci Search, BIOSIS Previews, Biological Abstracts, Focus on: Veterinary Science & Medicine, Zoological Record, PubMed /MEDLINE, Index Medicus, Pubmed Central, CAB Abstracts / Index Veterinarius, EBSCO, AGRIS and AGRICOLA. This journal published in English by the Korean Society of Veterinary Science (KSVS) being distributed worldwide.
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