Journal of photochemistry and photobiology. B, Biology最新文献

{"title":"Molecular mechanism underlying the modulated toxicity of differently charged and sized nanoplastics by bovine serum albumin","authors":"Xingren Pan ,&nbsp;Xiaozhang Yu ,&nbsp;Pengfei Qin ,&nbsp;Wanni Yu","doi":"10.1016/j.jphotobiol.2025.113337","DOIUrl":"10.1016/j.jphotobiol.2025.113337","url":null,"abstract":"<div><div>The extensively presence of nanoplastics raises concerns about their harm to ecosystems and human health. They are easy to absorb serum albumin to form corona during the transport process. However, the regulation of serum albumin to the toxicity of nanoplastics with differing charges and sizes remains unknown. We examined the molecular mechanism of polystyrene nanoplastics (PS-NPs) to bovine serum albumin (BSA) and how corona modulates the mouse primary hepatocyte damage. In cells exposed to 100 mg/L of large PS-NH₂-NPs and PS-COOH-NPs, a significant reduction in cell viability of 27 % and 22 % was noted, respectively, under BSA-free conditions. In contrast, the reductions were limited to 17 % and 13 % in the presence of BSA. Additionally, BSA pretreatment also lowered the reactive oxygen species (ROS) levels compared to the use of two modified PS-NPs (200 nm) alone. However, the addition of BSA did not significantly alter the cell viability or the levels of ROS in small PS-NPs (80 nm). Molecular investigations demonstrated that PS-NPs mainly bound to the hydrophobic cavity of BSA through moderate hydrophobic forces with the binding affinity approximately 10⁵ M⁻¹. The formation of corona not only induced conformational changes in BSA but also modified its esterase activity. The molecular and cellular experiments both revealed the size and surface charge-specific toxicity pattern of nanoplastics. Big PS-NPs (200 nm) bound more tightly to the protein compared to small nanoplastics (80 nm), and PS-NH₂-NPs presented a greater risk to BSA than PS-COOH-NPs. This study elucidates how BSA corona formation modulates nanoplastic toxicity in a size- and charge-dependent manner.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"274 ","pages":"Article 113337"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145768492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sonodynamic and photodynamic therapy, activated indocyanine green, and indocyanine green-loaded chitosan nanoparticle on resin tag length and shear bond strength to caries-affected dentin using two-step etch and rinse resin adhesive","authors":"Fahad Alkhudhairy","doi":"10.1016/j.jphotobiol.2025.113291","DOIUrl":"10.1016/j.jphotobiol.2025.113291","url":null,"abstract":"<div><h3>AIM</h3><div>Evaluating the effect of different disinfection protocols, i.e., Chlorhexidine (CHX), Sonodynamic therapy (SDT)-Indocyanine green (ICG), photodynamic therapy (PDT)-ICG, and ICG-loaded chitosan nanoparticles (CHNPs) on the resin tag length (RTL) and the shear bond strength (SBS) bonded to caries-affected dentin (CAD) using two step etch and rinse resin adhesive.</div></div><div><h3>Materials and methods</h3><div>The present study utilized fifty-six extracted human molars with carious lesions extending up to halfway between the pulp chamber and the enamel-dentin junction, corresponding to ICDAS criteria 5. All the samples were allocated into four groups based on the disinfection regimen (<em>n</em> = 14). Group 1: CHX, Group 2: ICG-PDT, Group 3: ICG-SDT, and Group 4: ICG-loaded CHNPs. A traditional two-step etch and rinse adhesive was used, followed by composite buildup. All specimens underwent thermocycling to replicate the aging effect. Scanning electron microscopy was used for characterization of chitosan nanoparticles and ICG-loaded CHNPs, followed by measurement of RTL (<em>n</em> = 4). Failure mode assessment and SBS analysis were performed using a stereomicroscope and universal testing machine(<em>n</em> = 10). ANOVA2 and post hoc Tukey test were conducted to statistically compare the RTL and SBS outcomes among various investigated groups, <em>p</em> ˂0.05.</div></div><div><h3>Results</h3><div>Group 3 (ICG-SDT) samples presented the maximum length of resin tags (<em>121.82 ± 5.23</em> μ<em>m)</em> and the highest bond strength (<em>9.43 ± 0.23 MPa)</em>. Whereas the minimum resin tag length was detected in Group 2 (ICG-PDT) (<em>59.21 ± 2.11</em> μ<em>m), along with the lowest bond strength</em> (<em>6.41 ± 0.92 MPa).</em> Comparative analysis between Group 1 (CHX) and Group 2 indicated that <em>no significant difference in their resin tag length and SBS</em> (p˃0.05). Similarly, intergroup analysis between Group 3 and Group 4 also demonstrated comparable bond strength and resin tags (p˃0.05).</div></div><div><h3>Conclusion</h3><div>Sonodynamic therapy with indocyanine green and indocyanine green-infused chitosan nanoparticles is an effective method for disinfecting caries-affected dentin, as it has shown appropriate resin tag length and bond strength.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"273 ","pages":"Article 113291"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145482322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Asperterol from the coral associated fungus Aspergillus terreus, which protects against UVB induced damage through antioxidant, anti-inflammatory, and barrier repair mechanisms","authors":"Guangming Tang ,&nbsp;Yi Zhang ,&nbsp;Zhong-Ji Qian","doi":"10.1016/j.jphotobiol.2025.113307","DOIUrl":"10.1016/j.jphotobiol.2025.113307","url":null,"abstract":"<div><div>Ultraviolet B (UVB) radiation, a primary cause of skin photoaging, triggers oxidative stress, inflammation, barrier dysfunction, and apoptosis in keratinocytes. New marine-derived benzaldehyde compound <strong>B-1</strong> (Asperterrol), isolated from the coral-associated fungus <em>Aspergillus terreus</em> C23–3, has been demonstrated to have multifaceted protective effects against UVB-induced photoaging in HaCaT keratinocytes. This study revealed that <strong>B-1</strong> restores cell viability at concentrations ranging from 2.5 to 10 μM and significantly reduces ROS overproduction, particularly at 10 μM, comparable to untreated controls. Mechanistically, <strong>B-1</strong> activated the Nrf2/HO-1 antioxidant pathway by promoting Nrf2 nuclear translocation and enhancing superoxide dismutase (SOD-1) expression, as evidenced by molecular docking showing stable hydrogen bonding with nuclear factor (Nrf2) residues (Val606 and Ile559). Concurrently, <strong>B-1</strong> suppressed ultraviolet radiation B (UVB)-triggered inflammation via dose-dependent inhibition of inhibitor of NF-kB alpha (IκBα) phosphorylation and NF-κB/MAPK signaling, reducing cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), interleukin-6 (IL-6), and IL-1β levels. Notably, <strong>B-1</strong> upregulated skin barrier proteins Filaggrin and Involucrin, thereby counteracting UVB-induced barrier dysfunction. Furthermore, <strong>B-1</strong> further mitigated apoptosis by normalizing the Bcl-2/Bax ratio and suppressing caspase-3 and caspase-9 activation while enhancing early-stage cell migration. These findings underscore the potential of <strong>B-1</strong> as a promising multitarget agent against UVB-driven skin damage, bridging marine fungal resources to dermatological innovation.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"273 ","pages":"Article 113307"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145517668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypericin-mediated photodynamic therapy promotes apoptosis and inhibits fibrosis by inducing HMOX1-mediated ferroptosis in hypertrophic scar fibroblasts","authors":"Xing Li ,&nbsp;Ziqing Chen ,&nbsp;Mingzhuo Liu,&nbsp;Jingwen An,&nbsp;Jinwei Shang,&nbsp;Gehua Zhu,&nbsp;Huicai Wen ,&nbsp;Guanghua Guo","doi":"10.1016/j.jphotobiol.2025.113303","DOIUrl":"10.1016/j.jphotobiol.2025.113303","url":null,"abstract":"<div><h3>Background</h3><div>The treatment of hypertrophic scars poses a significant therapeutic challenge due to the limitations of existing options. Given the inadequacy of current regimens, it is imperative to explore new and more effective treatment strategies.</div></div><div><h3>Objective</h3><div>Here, we investigated the mechanism by which hypericin-mediated photodynamic therapy (HYP-PDT) inhibits hypertrophic scar formation, with a focus on the role of HMOX1 and ferroptosis.</div></div><div><h3>Methods</h3><div>Using hypertrophic scar fibroblasts (HFs), we determined optimal HYP-PDT conditions and assessed its effects on cell proliferation, migration, apoptosis, and fibrosis. Integrated transcriptomic and proteomic analyses were performed to identify key targets, followed by mechanistic studies on ferroptosis.</div></div><div><h3>Results</h3><div>HYP-PDT significantly suppressed HF proliferation, migration, and fibrotic protein expression, while promoting apoptosis. Multi-omics analysis identified HMOX1 as a key upregulated target linked to ferroptosis. HYP-PDT increased ROS, lipid peroxidation, and Fe²⁺ levels—effects reversible by ferroptosis inhibitors. HMOX1 overexpression enhanced HYP-PDT-induced ferroptosis and apoptosis, but alone did not trigger these processes.</div></div><div><h3>Conclusion</h3><div>This study demonstrates that HYP-PDT induces ferroptosis via HMOX1 upregulation, providing a new mechanistic basis and potential therapeutic target for hypertrophic scar management.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"273 ","pages":"Article 113303"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145496043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyphenol-rich green propolis extract improves antioxidant and UV protection in sunscreen formulations","authors":"Michelli Ferrera Dario ,&nbsp;Flávia Sobreira Gonçalves ,&nbsp;Axelle Sogan ,&nbsp;Yanka Rodrigues Cardoso Barbosa ,&nbsp;Mariana Mandelli de Almeida ,&nbsp;Mauro Luciano Baesso ,&nbsp;Monique de Souza ,&nbsp;Fabio Galeotti ,&nbsp;Nicola Volpi ,&nbsp;André Rolim Baby ,&nbsp;Maria Valéria Robles Velasco","doi":"10.1016/j.jphotobiol.2025.113284","DOIUrl":"10.1016/j.jphotobiol.2025.113284","url":null,"abstract":"<div><div>The potential adverse effects, including systemic reactions, of some organic filters used in sunscreens have driven the search for alternatives. Brazilian green propolis, rich in polyphenols, shows promise in absorbing ultraviolet (UV) radiation and acting as an antioxidant in photoprotective formulations, allowing for a reduction in the concentration of organic filters while maintaining photoprotection efficacy. Oil-in-water emulsions were prepared containing chemical UV filters—ethylhexyl methoxycinnamate and butyl methoxydibenzoylmethane—with or without the addition of Brazilian green propolis extract at concentrations of 2.5 % and 5.0 % (<em>w</em>/w), along with a blank formulation as control. These formulations were assessed for antioxidant activity, photoprotective efficacy (in vitro and in vivo methods), photostability, inhibition of lipid peroxidation in the stratum corneum of human volunteers, and skin permeability. The addition of green propolis extract significantly enhanced both the antioxidant activity and sun protection factor (SPF) of the formulations without significant penetration, making it an excellent alternative to partially replace organic filters. In vitro photoprotective efficacy parameters met broad-spectrum sunscreen standards, providing good to excellent ultraviolet A (UVA) protection. However, the low photostability of the formulations limited the extract's ability to protect the stratum corneum from lipid peroxidation. Therefore, Brazilian green propolis serves as a promising adjuvant in sunscreen formulation, boosting efficacy while ensuring formulation safety.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"273 ","pages":"Article 113284"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145369835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Butyl toluidine blue as a photosensitizer for antimicrobial photodynamic therapy on titanium-associated biofilms","authors":"Amanda Paino Santana ,&nbsp;Daniela Moreira Cunha ,&nbsp;Rodolfo Debone Piazza ,&nbsp;Mark Wainwright ,&nbsp;Magda Feres ,&nbsp;Belen Retamal-Valdes ,&nbsp;Rafael Scaf de Molon ,&nbsp;Valdir Gouveia Garcia ,&nbsp;Leticia Helena Theodoro ,&nbsp;Wirley Gonçalves Assunção ,&nbsp;Erica Dorigatti de Avila","doi":"10.1016/j.jphotobiol.2025.113274","DOIUrl":"10.1016/j.jphotobiol.2025.113274","url":null,"abstract":"<div><div>This in vitro study aimed to determine a safe concentration of butyl toluidine blue (BuTB) and an appropriate pre-irradiation time for antimicrobial photodynamic therapy (aPDT) to reduce polymicrobial oral biofilm without causing cytotoxicity to human cells. Titanium (Ti) discs simulating implant abutment surfaces were inoculated with saliva from patients diagnosed with peri-implantitis to create a clinically relevant polymicrobial biofilm model. Antimicrobial PDT was performed using BuTB at 0.05 mg/mL and two pre-irradiation times (1 and 5 min), followed by diode laser irradiation (InGaAlP, 660 nm, 75 mW, 60 s, 4.5 J). The antimicrobial efficacy of each protocol was evaluated, and cytotoxicity was assessed on keratinocyte (HaCaT) and human gingival fibroblast (HGF) cell lines using both quantitative and qualitative approaches. Significant bacterial reduction was observed for both 1-min (8.91 ± 0.5 log₁₀ CFU/mL; <em>p</em> &lt; 0.0001) and 5-min (7.75 ± 0.6 log₁₀ CFU/mL; p &lt; 0.0001) pre-irradiation times, compared to untreated Ti controls (12.21 ± 0.1 log₁₀ CFU/mL). Although the 5-min protocol showed slightly greater antimicrobial activity (<em>p</em> = 0.0076), the 1-min protocol demonstrated favorable cell viability and mitochondrial activity in both HaCaT and HGF cells. These results suggest that BuTB-mediated aPDT, at a low concentration and short pre-irradiation time, can effectively reduce polymicrobial biofilm while preserving cell viability in the conditions studied. While further studies are needed, these in vitro findings provide a valuable foundation for developing safer and more effective aPDT protocols for potential future clinical applications.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"273 ","pages":"Article 113274"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145420180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and characterization of multifunctional mesoporous silica nanoparticles with dual targeting and dual-mode imaging for cancer therapy","authors":"Shiow-Yi Chen,&nbsp;Kian-Keat Kwek,&nbsp;Zui Harng Lee,&nbsp;Hsiu-Mei Lin","doi":"10.1016/j.jphotobiol.2025.113290","DOIUrl":"10.1016/j.jphotobiol.2025.113290","url":null,"abstract":"<div><div>This study investigated the innovative potential of multifunctional mesoporous silica nanoparticles (MSNs) as a novel drug carrier for cancer therapy. Dual-targeting was achieved by conjugating folic acid (FA) and glucose, enabling selective binding to folate receptors and glucose transporters on cancer cells. Europium (Eu³⁺) and gadolinium (Gd³⁺), were doped onto the MSN to provide fluorescence and MRI dual-mode imaging, while camptothecin (CPT) was attached <em>via</em> disulfide bonds for glutathione (GSH)-responsive release. Characterization confirmed the mesoporous structure and successful functionalization. <em>In vitro</em> assays showed that MSN-EuGd-CPT-Glu-FA exhibited good biocompatibility, maintaining over 80 % viability in normal L929 cells at 200 μg/mL, while significantly enhancing cytotoxicity toward HeLa cells, where viability decreased to ∼40 % and the IC₅₀ value dropped from 118.66 μg/mL for free CPT to 8.31 μg/mL. <em>In vivo</em> studies further validated the dual-mode imaging capability in mice using IVIS and MRI. These results demonstrate that the designed MSN system integrates targeted delivery, stimuli-responsive drug release, and diagnostic imaging, offering a promising multifunctional nanoplatform with the potential to enhance therapeutic efficacy while reducing side effects in cancer treatment.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"273 ","pages":"Article 113290"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145420179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light-induced reproductive responses in white-rumped munia (Lonchura striata)","authors":"Ruth Lalpekhlui,&nbsp;Lalsiamkima Hnamler,&nbsp;Amit Kumar Trivedi","doi":"10.1016/j.jphotobiol.2025.113300","DOIUrl":"10.1016/j.jphotobiol.2025.113300","url":null,"abstract":"<div><div>Photoperiod is a primary cue for most birds to initiate reproductive activities. How light influences the reproduction-linked processes in white-rumped munia is largely unknown. A series of experiments were performed on male birds. In experiment one, birds were exposed to either short (SD; 8 L: 16D) or long day length (LD; 15 L:9D) for one day and sampled at 19 h after light onset. In experiment two, birds were exposed to either SD (8 L: 16D), or equinox photoperiod (12 L:12D), or to LD (15 L:9D). In experiment 3, birds were exposed to an equinox photoperiod of 10 lx, 50 lx, or 100 lx. In experiment four, birds were exposed to an equinox photoperiod (0.5 W/m² of light irradiance) of red (RL; 650 nm) or blue light (BL; 450 nm). Experiment 2 to 4 ran for 30 days. For experiment five, birds were procured at four different times of the year, i.e., March, June, September, and December. Body mass and testicular volume size were observed in all experiments. In experiment 2 to 5 sampling was done during the middle of the light phase. Hypothalamic tissue was used for transcription studies of reproductive and epigenetic markers. In experiment 1, higher expressions of <em>Tshβ</em>, <em>Dio2</em>, <em>Eya3</em>, <em>Tet2</em>, and <em>Hat1</em> while lower expression of <em>Dnmt1</em> was observed in LD group. In experiment 2, higher expression of <em>Tshβ</em>, <em>Dio2</em>, <em>GnRh</em>, <em>Eya3</em>, and <em>Tet2</em> while lower expression of of <em>Dio3</em>, <em>GnIh</em>, <em>Hdac2</em>, <em>Dnmt1,</em> and <em>Dnmt3b</em> was observed in both 12 L:12D and 15 L:9D LD groups. In experiment 3, higher expression of <em>Tshβ</em>, <em>Dio2</em>, <em>GnRh</em>, <em>Tet2</em>, and <em>Hat1</em> while lower expression of <em>Dio3</em>, <em>GnIh</em>, <em>Hdac2</em>, and <em>Dnmt3b</em> was revealed in 50 and 100 lx groups. Experiment 4 resulted in higher expression of <em>Tshβ</em>, <em>Dio2</em>, <em>GnRh</em>, and <em>Tet2</em> while lower expression of <em>Dio3</em>, <em>GnIh</em>, <em>Hdac2</em>, <em>Dnmt1</em> and <em>Dnmt3b</em> in blue light group. Seasonal fluctuations were observed in all hypothalamic transcripts studied. Higher expression of <em>Tshβ</em>, <em>Dio2</em>, <em>GnRh</em>, <em>Eya3</em>, <em>Tet2</em>, and <em>Hat1</em> was revealed in September while <em>Dio3</em>, <em>GnIh</em>, <em>Hdac2</em>, <em>Dnmt1</em> and <em>Dnmt3b</em> were higher at other time of the year. The results from experiment 1 to 4 suggest that all three components of light i.e., duration, illuminance and spectral composition of light affects reproductive responses. While in nature season controls reproductive responses. Change in epigenetic transcripts suggest that and these responses are mediated by epigenetic mechanisms.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"273 ","pages":"Article 113300"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145468091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel 450 nm blue laser-mediated sinoporphyrin sodium-based photodynamic therapy inactivates Cutibacterium acnes through stress-mediated metabolic alterations","authors":"Hengtong Fan ,&nbsp;Zejun Ren ,&nbsp;Shuqi Chen ,&nbsp;Tingting Huang ,&nbsp;Guoxiong Liu ,&nbsp;Yashou Guo ,&nbsp;Yan Zheng ,&nbsp;Yifan Cheng ,&nbsp;Xing Li ,&nbsp;Yibo Mei ,&nbsp;Yuhang Chen ,&nbsp;Huihui Tuo ,&nbsp;Lijiang Gu ,&nbsp;Dalin He ,&nbsp;Jin Zeng","doi":"10.1016/j.jphotobiol.2025.113305","DOIUrl":"10.1016/j.jphotobiol.2025.113305","url":null,"abstract":"<div><div>Acne vulgaris is a chronic inflammatory skin disease closely linked to the abnormal colonization and proliferation of <em>Cutibacterium acnes</em> (<em>C. acnes</em>). Photodynamic therapy (PDT) has emerged as an ideal treatment. However, it still faces challenges such as low reactive oxygen species (ROS) production rates with porphyrin-based photosensitizers and low activation efficiency of conventional red light. This study investigated the <em>in vitro</em> and <em>in vivo</em> bactericidal effects of sinoporphyrin sodium (DVDMS) combined with a novel 450 nm blue laser-mediated photodynamic therapy (BL-PDT) on <em>C. acnes</em>, and explored the potential mechanisms, focusing on energy metabolism. In our results, <em>C. acnes</em> showed a time-dependent uptake of DVDMS, and BL-PDT demonstrated an excellent bactericidal effect on <em>C. acnes in vitro</em> by inducing a large amount of ROS production. RNA sequencing and metabolomic analysis revealed that BL-PDT inhibited <em>C. acnes</em> carbon metabolism while initially enhancing respiration; however, both fermentation and respiration were suppressed after 2 h, and ATP declined time-dependently in this process. Ultimately, the combined effects of ROS-induced damage (from DVDMS and enhanced respiration) and ATP depletion led to bacterial death. Similarly, <em>in vivo</em> experiments confirmed the favorable therapeutic efficacy and safety of BL-PDT in a rat model of acne. In conclusion, DVDMS-based BL-PDT may be a safe and effective new treatment against acne. Thus, our results provide compelling evidence for using DVDMS and BL-PDT in acne treatment.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"273 ","pages":"Article 113305"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145517628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Triphenylamine-based fluorescent probes for lipid droplet detection and non-alcoholic fatty liver disease identification","authors":"Jing-Yi Li ,&nbsp;Ya-Tong Liu ,&nbsp;Zhi-Bo Jiang,&nbsp;Si-Yi Yao,&nbsp;Fan-Qi Shen,&nbsp;Yu-Yang Wang,&nbsp;Yue-Li Zou,&nbsp;Li-Xia Zhao","doi":"10.1016/j.jphotobiol.2025.113301","DOIUrl":"10.1016/j.jphotobiol.2025.113301","url":null,"abstract":"<div><div>Lipid droplets (LDs) are important biomarkers for metabolic diseases such as non-alcoholic fatty liver disease (NAFLD), and they have significant research value in revealing the pathological mechanisms of these diseases. In this study, we designed three fluorescent probes based on triphenylamine, namely <strong>DM-1</strong>, <strong>DM-2</strong>, and <strong>DM-3</strong>, for the targeted detection of lipid droplets and the identification of NAFLD. <strong>DM1–3</strong> exhibits a unique response to small polar environments and has high sensitivity, high selectivity, large Stokes shift, high fluorescence quantum yield, a wide pH range (2–8), and rapid recognition. Particularly, the fluorescence intensity of probe <strong>DM-1</strong> at 576 nm (expressed in logarithmic form) has a good linear relationship with PBS/Diox (80–96 %) (R² = 0.9888). Therefore, probe <strong>DM-1</strong> was selected as the object for further study. The results of cell experiments indicated that the probe <strong>DM-1</strong> could target lipid droplets within cells and exhibited low cytotoxicity. In vivo experiments successfully achieved the imaging of LDs in the liver of NAFLD mouse models at different stages. Together, these results demonstrated the potential of probe <strong>DM-1</strong> as a polar-sensitive fluorescent tool for the early diagnosis and monitoring of NAFLD.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"273 ","pages":"Article 113301"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145496061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}