Paeoniflorin protected UVA-exposed skin fibroblasts from caspase-3/GSDME mediated pyroptosis

Background

UVA radiation can impact fibroblasts and leads to alterations in dermal connective tissue. Pyroptosis is a form of regulated cell death characterized by gasdermin-mediated membrane pore formation and release of intracellular contents. Whether UVA exposure can trigger pyroptosis in fibroblasts is unclear. Paeoniflorin has antioxidant properties, but whether it alleviates pyroptosis through inhibiting oxidative stress in fibroblasts is not yet determined.

Objectives

To identify the occurrence and the molecular mechanism of cell pyroptosis in skin fibroblasts, and explore the anti-pyroptotic effect of paeoniflorin in UVA induced photodamage protection.

Methods

Using Human skin fibroblasts (HSFs), wild-type primary human dermal fibroblasts (HDFs) and CASP3 knockdown HDFs, we analyzed the occurrence of pyroptosis by examining changes in morphology, LDH release and the expression of pyroptotic molecules after UVA radiation. UVA-exposed C57BL/6 mice and human sun-exposed skin samples were used to analyze the expression of caspase-3/GSDME. The role of oxidative stress was investigated by using NAC. Paeoniflorin treatment was analyzed for its effects on pyroptosis and the expression of caspase-3/GSDME.

Results

Both HSFs and HDFs exposed to UVA exhibited dose-dependent pyroptosis. In HDFs, UVA increased the expression of caspase-3, GSDME, PARP, caspase-9, Cytochrome C, Bax/Bcl-2, while the expression of NLRP3, caspase-1, caspase-4, GSDMD was not significantly changed. UVA-exposed C57BL/6 mice and human sun-exposed skin samples showed increased caspase-3/GSDME. Treatment of NAC or paeoniflorin suppressed UVA-induced pyroptosis and mitochondrial-mediated apoptosis.

Conclusions

UVA induced caspase-3/GSDME mediated pyroptosis in both HSFs and HDFs, and paeoniflorin protected against UVA induced photodamage by suppressing this pathway.