Journal of photochemistry and photobiology. B, Biology最新文献

{"title":"Wavelength-dependent photobiomodulation attenuates synovial inflammation in fibroblast-like synoviocytes and a collagenase-induced osteoarthritis model","authors":"Tianxiang Fan ,&nbsp;Peng Xia ,&nbsp;Safayet Ahmed ,&nbsp;Yuen H. Tsang ,&nbsp;Zhi Yao ,&nbsp;Deli Wang ,&nbsp;Ziyu Chen ,&nbsp;Lixiang Xue ,&nbsp;Dongquan Shi ,&nbsp;Bo Wang ,&nbsp;Chi Fai Cheung ,&nbsp;Marco Y.C. Pang ,&nbsp;Ye Li ,&nbsp;Siu Ngor Fu","doi":"10.1016/j.jphotobiol.2025.113276","DOIUrl":"10.1016/j.jphotobiol.2025.113276","url":null,"abstract":"<div><div>Photobiomodulation (PBM) using light-emitting diodes (LEDs) has demonstrated promising anti-inflammatory effects in musculoskeletal disorders. However, the optimal parameters for modulating synovitis and joint degeneration in osteoarthritis (OA) remain unclear. This study aimed to examine the wavelength- and dose-dependent effects of PBM on inflammatory responses in fibroblast-like synoviocytes (FLS) and evaluate the in vivo therapeutic efficacy of selected wavelengths in a murine model of collagenase-induced OA (CIOA). Primary rat FLS were stimulated with TNF-α and irradiated using PBM at 625, 810, 940, or 1050 nm with fluences ranging from 13 to 78 J/cm² (fixed irradiance of 44 mW/cm²). mRNA levels of IL-1β, IL-6, COX-2, and iNOS were quantified by RT-qPCR. The in vivo study involved daily PBM (810 or 940 nm at 39 J/cm²) for two weeks in CIOA mice, starting one week post-induction. Pain-related behavior was assessed weekly using the incapacitance test. Synovial inflammation and cartilage degradation were evaluated by histology and immunofluorescence staining for IL-1β and IL-6. Among tested parameters, 810 nm at 39 J/cm² showed the most potent suppression of inflammatory gene expression in FLS. In vivo, 810 nm PBM significantly improved pain-related behavior by week 4, reduced synovitis and cartilage damage, and downregulated IL-1β and IL-6 expression in synovium. While 940 nm PBM also demonstrated anti-inflammatory effects in vitro, it did not show significant efficacy in vivo. These findings suggest that PBM at 810 nm exerts stronger anti-inflammatory and chondroprotective effects in inflammatory OA compared with 940 nm, supporting its potential as a non-pharmacological intervention for inflammatory OA phenotypes.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113276"},"PeriodicalIF":3.7,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145266693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wavelength-dependent excitation ratio of photosystem I and II in Arabidopsis thaliana","authors":"Peter R. Bos,&nbsp;Herbert van Amerongen,&nbsp;Emilie Wientjes","doi":"10.1016/j.jphotobiol.2025.113278","DOIUrl":"10.1016/j.jphotobiol.2025.113278","url":null,"abstract":"<div><div>Photosynthesis is a finely tuned process in which plants balance excitations between photosystem I (PSI) and photosystem II (PSII) to optimize energy conversion efficiency. The distribution of light energy between PSI and PSII across the full spectrum of photosynthetically active radiation is hard to quantify. Current attempts rely on estimating the PSI/PSII reaction center ratio and PS antenna sizes. In this study, we employed a streak-camera system to measure excitation distribution between PSI and PSII in <em>Arabidopsis thaliana</em> leaves over the wavelength range 430–630 nm, bypassing the need for indirect estimations of antenna size and pigment distribution. Our findings show that the absorption weighted PSII/(PSI + PSII) excitation ratio is 0.60 ± 0.01 in the 430–630 nm spectral range. By fitting the excitation spectrum with absorption spectra of PSI, PSII, and LHCII, we estimate the PSII/(PSI + PSII) reaction center ratio to be 0.58 ± 0.004. The excitation ratio shows that in the dark-adapted supercomplex organisation, PSII is overexcited. By recording a light response curve of ΦPSI and ΦPSII, we determined that the dark-adapted supercomplex organisation leads to overexcitation of PSII in low to medium light illumination intensities (∼372 μmol m⁻² s⁻¹). However, state transitions alone can rebalance the charge separation ratio. The quantitative excitation ratio and its correlation with reaction center ratios provide crucial parameters for refining photosynthetic models and understanding energy distribution across photosystems.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113278"},"PeriodicalIF":3.7,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145267345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short-duration UV-B exposure mitigates salinity stress in Withania somnifera (L.) Dunal and boosts secondary metabolite production","authors":"Sneha Singh,&nbsp;Pajeb Saha,&nbsp;Sabitri Kumari,&nbsp;Jyotiraj Upadhyay,&nbsp;Shashi Pandey-Rai","doi":"10.1016/j.jphotobiol.2025.113279","DOIUrl":"10.1016/j.jphotobiol.2025.113279","url":null,"abstract":"<div><div><em>Withania somnifera</em> is a widely valued medicinal plant traditionally cultivated in arid and semi-arid regions of India, where salt stress and rising UV-B radiation pose growing threats to its productivity and pharmacological quality of different metabolites. While the individual impacts of salinity and UV-B stress have been extensively studied, their interactive effects and potential for cross-tolerance in <em>W. somnifera</em> remain unexplored. In this study, we investigated the morphophysiological, biochemical, and metabolic responses of <em>W. somnifera</em> under salt (50–200 mM NaCl), UV-B (1–4 h), and combined exposure. Salt stress significantly reduced plant height, shoot biomass, photosynthetic efficiency (Fv/fm), and pigment content, while inducing oxidative damage. UV-B exposure alone enhanced relative water content, stimulated antioxidant enzyme activities (SOD, CAT, APX, and GR), and promoted the accumulation of secondary metabolites. Under combined salt and UV-B exposure, plants exhibited further amplified responses wherein antioxidant enzyme activities remained elevated while lipid peroxidation levels decreased compared to salt stress alone, suggesting reduced oxidative damage. Due to short-term UV-B exposure, metabolite profiling <em>via</em> high-resolution mass spectrometry revealed a synergistic enhancement in the accumulation of key secondary metabolites, particularly withanolides, flavonoids and triterpenoids, alongside the unique induction of stress-responsive compounds such as betaine and arjungenin. These findings suggest that short-duration UV-B exposure synergistically interacts with salt stress, intensifying redox activity and metabolic reprogramming to reinforce the plant's adaptive capacity. This work provides a foundation for integrating UV-B-based agronomic strategies in saline-prone regions to boost both stress resilience and the commercial phytochemical yield of <em>W. somnifera</em>.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113279"},"PeriodicalIF":3.7,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145258505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photosensitization and phototoxicity mechanism of diclofenac sodium (NSAID) under ambient UVR exposure.","authors":"Gaurav Prajapati, Sunil Kumar Patel, Durgesh Mourya, Meetali Sinha, Mohd Danish Kamar, Ratan Singh Ray, Ashish Dwivedi","doi":"10.1016/j.jphotobiol.2025.113227","DOIUrl":"10.1016/j.jphotobiol.2025.113227","url":null,"abstract":"<p><p>Diclofenac sodium (DS) is used for pain, inflammation and musculoskeletal disorders. Despite its absorption of UV radiation, the phototoxic potential and molecular mechanisms in keratinocytes under UVA and UVB exposures are largely unexplored. In this study, we have employed a comprehensive array of in-silico, in-chemico, and in-vitro approaches to assess DS's photostability, phototoxic, and photogenotoxic properties under UV irradiation. Our findings indicate that DS undergoes photodegradation, forming 3 photoproducts. Further, docking studies revealed DS's affinity for DNA and Bax, implicating DNA damage and initiation of programmed cell death pathways. MTT and NRU assays demonstrated a concentration-dependent phototoxic response to UVA and UVB exposures. DCFH₂-DA and DHE staining confirmed that DS significantly elevates ROS under UVR. Photogenotoxicity was evidenced by comet assay, while clastogenic activity was confirmed through micronucleus and chromosomal aberration assays. Gene expression analysis showed upregulation of apoptotic marker genes including Bax, Bcl2, Casp9, Apaf1, Casp3, and Cyt C in keratinocytes treated with DS under UV irradiation. Our results showed that DS exhibits both photogenotoxic and photoclastogenic activities, coupled with ROS-induced programmed cell death, elucidating the molecular basis of its phototoxicity. These insights are crucial for understanding the mechanisms behind NSAID-induced phototoxic skin diseases under environmental UVR exposure.</p>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"271 ","pages":"113227"},"PeriodicalIF":3.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the potential of spectral LED lights in enhancing glandular trichome density and accumulation of bioactive specialized metabolites in spearmint.","authors":"Dipanjali Chatterjee, Adinpunya Mitra","doi":"10.1016/j.jphotobiol.2025.113239","DOIUrl":"10.1016/j.jphotobiol.2025.113239","url":null,"abstract":"<p><p>Spearmint is a well-known aromatic plant recognized for its unique aroma, flavour, and bioactive properties. To ensure its year-round production in response to the growing market demand under rapidly changing climates, the implementation of light-emitting diodes (LEDs) in controlled environments presents a promising solution. This study explored the impact of different LED spectral lights, namely monochromatic red (R), monochromatic blue (B), combined red-blue (RB), and control warm white (WW) on the vegetative growth, glandular trichome density, key specialized metabolite contents and relevant biosynthetic gene expression levels in spearmint. Various morphometric features showed improvement under RB and B lights compared to WW, while R light resulted in overall impaired growth. Plants under RB light showed the highest density of peltate glandular trichomes (43 Nos./mm²), which exhibited strong positive correlation with maximally elevated levels of marker terpene carvone (9.2 μmol/g FM) and its precursor limonene (0.5 μmol/g FM). This suggested a possible interlinkage between trichome development and terpene biosynthesis. Additionally, rosmarinic acid content significantly increased (781.5 μg/g FM) under RB light compared to other spectral light conditions. The highest expression levels of genes related to both phenolic and monoterpene biosynthetic pathways were also obtained under RB light, explaining the enhanced production of key bioactive compounds at the transcript level. Thus, the combination of R and B lights proved advantageous for the growth of spearmint and the concurrent enrichment of major specialized metabolites. The insights gained from this research will pave ways for developing appropriate LED-based cultivation systems for commercial production of spearmint.</p>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"271 ","pages":"113239"},"PeriodicalIF":3.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144817017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between ultraviolet exposure and type 2 diabetes: Insights from the China Health and Retirement Longitudinal Study (CHARLS).","authors":"Can Ming, Zhuoyu Liu, Wei Wu, Qian Gao","doi":"10.1016/j.jphotobiol.2025.113240","DOIUrl":"10.1016/j.jphotobiol.2025.113240","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the association between long-term exposure to ambient ultraviolet radiation (UVR) and the risk of developing type 2 diabetes (T2D).</p><p><strong>Research design and methods: </strong>This study analyzed data from 14,825 participants enrolled in the 2015 China Health and Retirement Longitudinal Study (CHARLS). Ground-based daily average UVR monitoring data, collected from January 1, 2005, to December 31, 2014, across 724 stations in China, were matched to 125 cities corresponding to participants' residences. UVR exposure levels were calculated separately for annual, spring, summer, autumn, and winter. All analyses were stratified by sex. Initially, a generalized linear mixed-effects model (GLMM) was employed to assess the association between city-level UVR exposure and individual-level risk of T2D. Further stratified analyses were performed based on age, body mass index (BMI), and urban versus rural residence.</p><p><strong>Results: </strong>Higher UVR exposure was associated with a lower risk of T2D in women (OR_annual: 0.250, 95 % CI: 0.081-0.773; OR_spring: 0.437, 95 % CI: 0.194-0.984; OR_autumn: 0.305, 95 % CI: 0.098-0.944; OR_winter: 0.227, 95 % CI: 0.079-0.655). Stratified analyses revealed that annual UVR exposure had a strong protective effect in T2D among women aged under 60 years (OR: 0.163, 95 % CI: 0.034-0.792), normal and underweight women and men (OR_women: 0.080, 95 % CI: 0.017-0.383; OR_men: 0.180, 95 % CI: 0.038-0.850) and rural women (OR: 0.207, 95 % CI: 0.065-0.661).</p><p><strong>Conclusions: </strong>Exposure to UVR appears to confer a protective effect against T2D, with this association being especially evident in women. The protective influence is more marked among individuals younger than 60 years, those with normal weight or underweight, and those residing in rural areas.</p>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"271 ","pages":"113240"},"PeriodicalIF":3.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144817016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quinolinium-substituted porphyrins as promising antimicrobial photosensitizers","authors":"Leticia D. Costa ,&nbsp;Cátia Vieira ,&nbsp;Mariana Q. Mesquita ,&nbsp;Steffen Hackbarth ,&nbsp;M. Graça P.M.S. Neves ,&nbsp;Adelaide Almeida ,&nbsp;Augusto C. Tomé ,&nbsp;M. Amparo F. Faustino","doi":"10.1016/j.jphotobiol.2025.113275","DOIUrl":"10.1016/j.jphotobiol.2025.113275","url":null,"abstract":"<div><div>Characterized by a remarkable chemical versatility and outstanding photophysical properties, porphyrins stand out as one of the most promising classes of photosensitizers for antimicrobial photodynamic therapy (aPDT). In this work, we described the synthesis, as well as the chemical and photophysical characterization of quinolinium-substituted porphyrins, and their corresponding Zn(II) complexes. The efficacy of these porphyrins in the photoinactivation of bacteria was evaluated, both alone and combined with the co-adjuvant potassium iodide, against methicillin-resistant <em>Staphylococcus aureus (</em>MRSA, Gram-positive) and <em>Escherichia coli</em> (Gram-negative) strains.</div><div>Overall, the results demonstrated the high potential of both cationic porphyrins to effectively photoinactivate bacterial strains. Cationic derivative <strong>2</strong>, at a concentration of 1.0 μM, achieved reductions exceeding &gt; 99.99999 % (&gt;7.0 log₁₀ CFU mL⁻¹) for <em>S. aureus</em> after 10 min of white light irradiation (25 mW cm⁻²) and &gt; 99.9 % (3.52 log₁₀ RLU) for <em>E. coli</em> after 60 min aPDT treatment. Similarly, the Zn(II) counterpart <strong>2a</strong> also showed excellent efficiency, achieving ∼99.999 % reduction (4.95 log₁₀ CFU mL⁻¹) against MRSA after 60 min of aPDT at 1.0 μM, and a 3.52 log₁₀ RLU reduction for <em>E. coli</em> after 30 min of PDT at 5.0 μM. The action mechanism of both cationic quinolinium-substituted porphyrins demonstrated to be dependent on the singlet oxygen production, consistent with a Type II photodynamic pathway. The use of potassium iodide (KI) as co-adjuvant led to the production of reactive iodine species (RIS), namely iodine, and increased the photodynamic effect.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113275"},"PeriodicalIF":3.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145251346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of near-infrared-triggered photothermal therapy on collagen organization and wound healing","authors":"Yan Chen ,&nbsp;Chuanjie Zhang ,&nbsp;Xiuhong Weng ,&nbsp;Shengjun Jiang ,&nbsp;Bo Cheng","doi":"10.1016/j.jphotobiol.2025.113273","DOIUrl":"10.1016/j.jphotobiol.2025.113273","url":null,"abstract":"<div><div>Soft tissue wounds result in the loss of essential functions, including sensation and barrier protection. Mild photothermal therapy (PTT) has emerged as a promising therapeutic strategy for promoting wound healing and tissue regeneration, primarily through its ability to precisely regulate tissue temperature and thereby influence cellular proliferation and differentiation. In this study, gold nanorods (AuNRs) were employed as photosensitizers and co-cultured with fibroblasts to evaluate the effects of mild PTT on collagen organization and wound healing under the irradiation of 808 nm near-infrared (NIR) laser. AuNRs-mediated photothermal stimulation raised the temperature of the culture medium from 18.0 °C to 37.8 °C within 7 min. This controlled thermal stimulation significantly enhanced fibroblast proliferation and led to a more random distribution of collagen fibers. Moreover, AuNR-mediated mild PTT promoted the differentiation of fibroblast into myofibroblasts and reduced scar tissue formation. These findings highlight the therapeutic potential of mild PTT in soft tissue regeneration by modulating fibroblast behavior and remodeling collagen architecture.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113273"},"PeriodicalIF":3.7,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A theoretical model for optimizing UVA/riboflavin crosslinking","authors":"Shaowu Sun ,&nbsp;Xiaona Liu ,&nbsp;Jiayi Dong ,&nbsp;Xiaoyu Zhao ,&nbsp;Chenxi Zhang ,&nbsp;Chenyan Wang ,&nbsp;Xiaolu Ma ,&nbsp;Yan Wang ,&nbsp;Xiaona Li ,&nbsp;Zhipeng Gao ,&nbsp;Weiyi Chen","doi":"10.1016/j.jphotobiol.2025.113272","DOIUrl":"10.1016/j.jphotobiol.2025.113272","url":null,"abstract":"<div><div>Ultraviolet-A and riboflavin (UVA/R) crosslinking has emerged as a valuable technique for ocular disease treatments. However, the exact influence of the oxygen and riboflavin on the crosslinking has not been fully explored so far. A kinetic model was developed to predict corneal/scleral stiffening effects under varying oxygen levels, irradiation intensities, and riboflavin solution concentrations during crosslinking at a fixed fluence. The optical properties of porcine sclera were determined by UV–Vis spectrophotometry. Monte Carlo method was employed to evaluate intrastromal light absorption of riboflavin. The roles of oxygen and riboflavin in the reaction mechanism were proposed based on the kinetic interactions of reactive species within the corneal/scleral stroma. Kinetic simulations indicated that increasing intrastromal oxygen concentration, driven by higher ambient oxygen levels, significantly improves crosslinking efficiency. The crosslinker formation rate reach its maximum when the intrastromal riboflavin concentration was approximately 0.245 %. Concentrations that are either too low or too high are adversely affect the formation of crosslinker. Optimal stiffening effects could be achieved by balancing oxygen availability, irradiation intensity, and riboflavin solution concentration. The model accuracy of oxygen prediction was verified by experimental results obtained from corneal crosslinking. Linear correlations were found between the model-predicted concentration of newly induced crosslinkers and the experimentally measured mechanical properties of both the cornea and sclera under various crosslinking protocols. Our study improved the prediction model by introducing accurate optical properties of the sclera. This proposed model provides a possibility for predicting the biomechanical crosslinking efficacy of the cornea/sclera, and may be used for optimizing UVA/R crosslinking protocols in customized treatment for ocular disease.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113272"},"PeriodicalIF":3.7,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145266708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Near-IR quantum dot (BagQD) as fluorescent sensor for live-cell imaging and in-vivo antibacterial photocatalytic therapy (APT)","authors":"Subhendu Sekhar Bag ,&nbsp;Sayantan Sinha ,&nbsp;Aniket Banerjee ,&nbsp;Hemanshu Mediboyana ,&nbsp;Animes K. Golder","doi":"10.1016/j.jphotobiol.2025.113271","DOIUrl":"10.1016/j.jphotobiol.2025.113271","url":null,"abstract":"<div><div>The development of multitasking agents that can sense, as well as damage multidrug-resistant (MDR) bacteria with no growth, is the need of the hour to overcome the increased mortality associated with antibacterial resistance. Toward this end, to overcome the limitation of organic dyes as fluorescence probes, and other developed quantum dots (QD), we present new of its kind near infrared type-I (NIR-I) CdSe/ZnSe/FeTiO₃-based perovskite quantum dot, ^{<strong><em>Bag</em></strong>}<strong><em>QD</em></strong>, for fluorescence-guided selective bacterial imaging-sensing and permanent disinfection. ^{<strong><em>Bag</em></strong>}<strong><em>QD</em></strong> possesses unique photodynamic and photophysical properties of the near-infrared NIR-I biological window. The excellent electronic transitions of ^{<strong><em>Bag</em></strong>}<strong><em>QD</em></strong> resulted in efficient quantitative fluorescent sensing-imaging of gram-positive (G+ve) as well as gram-negative (G-ve) bacterial pathogens, and complete in-situ and in-vivo disinfection/elimination of pathogenic bacteria without regrowth via damaging bacterial cell wall and cellular DNA, by ROS generation during photocatalysis. The material cytotoxicity study showed no harm to the human cell line. Moreover, the therapeutic application of ^{<strong><em>Bag</em></strong>}<strong><em>QD</em></strong> on in-vivo mice skin wound infection, revealed the generation of hair follicles and epithelization within 6–8 days. Thus, ^{<strong><em>Bag</em></strong>}<strong><em>QD</em></strong> serves as a new alternative to antibiotics and a smart nanomedicine that can uphold the hope in revolutionizing diagnostics and clinical avenues.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113271"},"PeriodicalIF":3.7,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145220751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}