Journal of photochemistry and photobiology. B, Biology最新文献

{"title":"Synergistic effects of oral milk ceramide-collagen peptides mixtures in preventing UV-induced inflammation and photoaging through TGF-β and NF-κB/MAPK signaling pathways in UV-exposed hairless mice","authors":"Sung Hee Han ,&nbsp;Hyung Joo Suh ,&nbsp;Sang Jun Lee ,&nbsp;Yeok Boo Chang","doi":"10.1016/j.jphotobiol.2025.113171","DOIUrl":"10.1016/j.jphotobiol.2025.113171","url":null,"abstract":"<div><div>This study investigates the synergistic effects of oral milk ceramide-collagen peptides in inhibiting UV-induced inflammation and preventing photoaging. The optimal ratio of milk ceramide to collagen peptides was determined in HaCaT cells, and the effects of oral supplementation of milk ceramide-collagen peptides were evaluated in UV-exposed hairless mice. HaCaT cells did not exhibit cytotoxicity when treated with milk ceramide and collagen peptides at concentrations up to 200 μg/mL. UVB exposure decreased cell viability, but treatment with the milk ceramide-collagen peptides mixtures (1:1, 1:3) prevented further viability loss and improved collagen peptides synthesis markers, including MMPs and TIMPs. The combination also enhanced moisture-related factors (AQP3, FLG) and reduced inflammatory cytokines (IL-6, IL-1β) and COX expression. In hairless mice, oral supplementation of milk ceramide-collagen peptides mixture (1:1 ratio) improved skin hydration, reduced erythema, TEWL, skin thickness, and wrinkle formation in a dose-dependent manner. The treatment also suppressed the expression of MMPs and TIMPs, promoting collagen peptides synthesis. Furthermore, the mixtures regulated NF-κB and MAPK signaling pathways, reducing inflammation and photoaging. These results suggest that the 1:1 milk ceramide-collagen peptides mixture effectively prevents UV-induced skin damage and photoaging by enhancing collagen peptides production and improving skin barrier function.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"268 ","pages":"Article 113171"},"PeriodicalIF":3.9,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143902103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Binding of collagen I to integrins alleviates UVB-caused mitochondrial disorders in human keratinocytes HaCaT through enhancement of F-actin polymerization","authors":"Yuying Zhu ,&nbsp;Tianyu Ji ,&nbsp;Zhao San ,&nbsp;Ruiyi Cao ,&nbsp;Toshihiko Hayashi ,&nbsp;Kazunori Mizuno ,&nbsp;Shunji Hattori ,&nbsp;Hitomi Fujisaki ,&nbsp;Weiwei Liu ,&nbsp;Takashi Ikejima","doi":"10.1016/j.jphotobiol.2025.113170","DOIUrl":"10.1016/j.jphotobiol.2025.113170","url":null,"abstract":"<div><div>Collagen I is one of the major components of the extracellular matrix in human skin, and is frequently used in skin cares and medications. Previously, we revealed that human keratinocytes HaCaT cells grown on collagen I (Col)-coated dishes gain resistance against UVB damages owing to the restored mitophagy. In this study, we further investigate the mechanisms by which collagen I modulates mitophagy. UVB irradiation causes loss of integrin β1 and collapse of F-actin cytoskeleton. Considering the requirement of actin skeleton in various cellular processes, we are curious about the participation of F-actin collapse in UVB damage. Integrin β1, whose activation enhances F-actin assembly, is a potential target for Col in UVB-treated cells. Notably, inhibiting integrin by adding an inhibitor RGDS or siRNA attenuates the effect of Col against UVB damages, confirming the participation of integrin in cell protection. The collapse of F-actin is rescued by Col, accompanying increases in the mRNA of F-actin polymerization-associated proteins and decreases in the mRNA of depolymerization-associated proteins. Inhibiting actin polymerization by using cytochalasin D represses the protective effect of Col, confirming the cytoprotective role of F-actin in UVB-treated cells. Remarkably, mitophagy in UVB-treated cells restored by Col-coating is inhibited by adding cytochalasin D or RGDS, as shown by the decreases of lysosomes, mitochondrial ubiquitin proteins, and co-localization of autophagosomes and mitochondria, resulting in accumulation of damaged mitochondria, which stresses the importance of F-actin and integrin in mitophagy. In summary, integrins and F-actin are required for mitophagy in UVB-irradiated HaCaT cells, and their enhancements by Col-coating facilitate timely elimination of damaged mitochondria caused by UVB, finally contributing to cell survival.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"268 ","pages":"Article 113170"},"PeriodicalIF":3.9,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143908233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating photosystem II efficiency in Parachlorella kessleri under atrazine exposure using chlorophyll a fluorescence analysis","authors":"Brian Ospina Calvo ,&nbsp;Ángela B. Juárez ,&nbsp;M. Gabriela Lagorio","doi":"10.1016/j.jphotobiol.2025.113167","DOIUrl":"10.1016/j.jphotobiol.2025.113167","url":null,"abstract":"<div><div>This study aims to evaluate the toxicity of Atrazine on freshwater algae by employing spectroscopic analysis (specifically the F₆₈₃/F₇₃₀ ratio) and photosynthetic parameters (Fv/Fm, Fv/F₀, ФC, ФPSII, qP, and UQF) as bioindicators. The unicellular alga <em>Parachlorella kessleri</em> was selected for this investigation. Cells of <em>Parachlorella kessleri</em> were cultivated under axenic conditions in Bold's Basal Medium (BBM) and subsequently exposed to various concentrations of Atrazine (260, 520, and 1040 μg/L) for three distinct exposure durations (1, 60, and 360 min). Reflectance, transmittance, basal steady-state emission spectra, and variable chlorophyll-<em>a</em> fluorescence were recorded and analyzed for both control and treated algae groups. The results demonstrated that Atrazine inhibits electron transport in the photosynthetic chain of <em>Parachlorella kessleri</em>, leading to a marked decline in photosynthetic efficiency. The absorbed light energy was predominantly dissipated via the physical de-excitation pathway (ΦC), while regulated heat dissipation remained minimal—even in the absence of the herbicide—suggesting a possible deficiency in the PGR5-dependent photoprotective pathway. Among the tested fluorescence parameters, ΦPSII, derived from Kautsky kinetics, proved to be the most sensitive to Atrazine exposure, highlighting its potential use in algae-based biosensors for herbicide detection in aquatic environments. Although the OJIP method provided a faster response and detected abrupt changes in relative chlorophyll fluorescence (VOP), these changes did not correlate with herbicide concentration. Steady-state fluorescence, especially when corrected for inner filter effects, may offer a complementary, non-invasive tool for monitoring aquatic stressors. However, anomalous responses at high herbicide concentrations indicated the need for further validation under varied conditions.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"267 ","pages":"Article 113167"},"PeriodicalIF":3.9,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological and biochemical analysis along with gene expression dynamics in Lilium ‘Brunello’ under supplemented effect of blue and red light treatment in pin tray soil-less cultivation system","authors":"Mahinder Partap ,&nbsp;H.N. Deekshith ,&nbsp;Himanshi Gupta ,&nbsp;Gulshan Birsanta ,&nbsp;Payal Kapoor ,&nbsp;Vikas Soni ,&nbsp;Bhavya Bhargava","doi":"10.1016/j.jphotobiol.2025.113168","DOIUrl":"10.1016/j.jphotobiol.2025.113168","url":null,"abstract":"<div><div>The present research corroborates the influence of supplementation effect of blue light (BL), red light (RL), and the combination of both BL + RL on the morphological traits, biochemical, and expression of key flowering genes assessment in lilium cultivated in bulb forcing soil-less pin trays within a climate-controlled autonomous greenhouse system. The findings revealed profound effect of BL + RL treatment on increased plant height (79.59 cm), stem diameter (10.05 mm), bud length (93.73 mm), and bud diameter (29.87 mm), compared to individual BL and RL treatments and the white light (WL) control. The BL + RL treatment also resulted in shortening the flowering timeline. Maximum flower diameter (18.65 cm) and chlorophyll content index (48.60) were recorded under BL + RL treatment. BL + RL extended post-harvest longevity to 13.2 days, followed by RL (11.6 days). Total chlorophyll content highest at the color bud stage under BL + RL (499.87 μg g⁻¹), though RL treatment sustained the highest levels at the flower stage. Carotenoids content showed a contrasting trend, with RL promoting maximum accumulation during bud stages, while BL + RL enhanced carotenoid synthesis at the flower stage (166.88 μg g⁻¹). Phenol and flavonoid content also reached maximum levels under BL + RL at the color bud stage and anthocyanin content was highest under BL + RL at the flower stage. Additionally, antioxidant activity was significantly higher (86.75 %) in flowers grown under BL + RL. Similarly, up-regulation of flowering genes <em>FT</em> and <em>CO9</em>, particularly observed under the BL + RL treatment at the initial bud and color bud stages, while flowering repressor <em>FLC</em> was down-regulated under all light treatments, facilitating earlier floral induction. The combined BL + RL treatment enhances <em>Lilium</em> cultivation profitability by improving growth, accelerating flowering, increasing flower quality and shelf life, and enabling faster production cycles in controlled Soil-less systems.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"267 ","pages":"Article 113168"},"PeriodicalIF":3.9,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biophysical effects of Phellinus Linteus on hepatocellular carcinoma cells studied by atomic force microscopy","authors":"Yuxi Huang ,&nbsp;Fan Yang ,&nbsp;Chuanzhi Liu ,&nbsp;Guicai Song ,&nbsp;Zuobin Wang","doi":"10.1016/j.jphotobiol.2025.113169","DOIUrl":"10.1016/j.jphotobiol.2025.113169","url":null,"abstract":"<div><div>Precisely quantifying cancer cell morphology and physical properties is crucial for advancing cancer therapeutics, drug screening, and pharmacological assessment. We employed atomic force microscopy (AFM) in this study to accurately measure the morphological and mechanical characteristics of cells and to observe drug-induced cell reactions. To observe the morphological and mechanical properties of both living and fixed HL-7702 cells and SMCC-7721 cells at the nanometer scale. Scanning Electron Microscopy (SEM) analysis revealed that treatment with Phellinus linteus water extract (PLWE) induced a morphological transition in SMCC-7721 cells from an irregular, flattened state to a more rounded and spherical shape, with a significant reduction in membrane structures. These changes are closely associated with apoptotic processes. The morphological changes observed in SMCC-7721 cells, together with MTT assay results, suggest that PLWE possesses substantial antitumor activity against cancer cells while exhibiting low toxicity towards normal cells, highlighting its potential for cell-selective targeting in cancer therapy. This study enhances our understanding of PLWE as an anticancer drug and contributes to novel strategies for precision medicine and personalized individualized treatment.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"267 ","pages":"Article 113169"},"PeriodicalIF":3.9,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143875035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pre-sowing magnetic treatments of seeds improve photosynthetic performance of two photosystems in soybean plants","authors":"Rupal Singh Tomar ,&nbsp;Pinke Patel ,&nbsp;Prabha Rai Kalal ,&nbsp;Pratibha Laad ,&nbsp;K.N. Guruprasad ,&nbsp;Anjana Jajoo","doi":"10.1016/j.jphotobiol.2025.113166","DOIUrl":"10.1016/j.jphotobiol.2025.113166","url":null,"abstract":"<div><div>To investigate the impact of pre-sowing magnetic treatment on soybean plants under natural conditions, a study was carried out focusing on the photosynthetic machinery. Soybean seeds were subjected to magneto-priming through exposure to pulsed magnetic field (PMF, 200 mT for 1 h) and static magnetic field (SMF, 200 mT for 1 h). The dual pulse-amplitude modulator (PAM) technique was employed to assess the simultaneous performance of photosystem II (PSII) and photosystem I (PSI) in magneto-primed soybean seeds. It was observed that after magnetic treatment (MT), the effective quantum yield of photosystem II [Y(II)] exhibited an increase, accompanied by a reduction in non-photochemical quenching (NPQ). Furthermore, the quantum yield of photosystem I [Y(I)] showed enhancement, reaching its maximum in the 200 mT PMF treatment. Non-photochemical quenching was lower in MT-treated seed plants as compared to control, indicating a reduction in energy dissipation and an enhancement in energy transfer from PSII to PSI. The magneto-primed seeds demonstrated an increased electron transport mechanism as evidenced by enhanced ETR(I) and ETR(II). Additionally, lower values of Y(NA) and Y(ND) suggested improved activity of both PSI and PSII in regulating energy transport in cyclic electron flow (CEF) and linear electron flow (LEF). The results suggest that PMF treatment is more beneficial than SMF for enhancing photosynthetic efficiency via regulation of PS II and PSI quantum yield.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"267 ","pages":"Article 113166"},"PeriodicalIF":3.9,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143875034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic insights into temoporfin-based photodynamic therapy: Ferroptosis as a critical regulator under normoxic and hypoxic conditions in head and neck cancer","authors":"Tong-Hong Wang ,&nbsp;Li-Fang Chou ,&nbsp;Yen-Wen Shen ,&nbsp;Nan-Chin Lin ,&nbsp;Yin-Hwa Shih ,&nbsp;Tzong-Ming Shieh","doi":"10.1016/j.jphotobiol.2025.113165","DOIUrl":"10.1016/j.jphotobiol.2025.113165","url":null,"abstract":"<div><div>Temoporfin is a second-generation photosensitizer used in photodynamic therapy (PDT) for the clinical treatment of head and neck cancer. However, its role in inhibiting cancer cell viability under normoxic and hypoxic conditions remains unclear. The oral squamous cell carcinoma (OSCC) cell lines, SAS and OECM-1 were cultured under normoxic or hypoxic conditions to investigate temoporfin-based PDT-induced cell death and the underlying mechanisms. Cell viability was analyzed using the MTT assay. Intracellular reactive oxygen species (ROS) levels, cell apoptosis, intracellular ROS, iron levels, lipid peroxidation, and glutathione (GSH) levels were assessed by flow cytometry. The expression of proteins related to oxidative stress, apoptosis, autophagy, and ferroptosis was verified by western blotting. Results showed that increasing the temoporfin dose, absorption time, and illumination time was positively correlated with the inhibition of oral cancer cells. Hypoxic conditions attenuated the toxicity of temoporfin in cancer cells. OECM-1 cells were more sensitive to temoporfin than SAS cells. Temoporfin-based PDT-induced ROS exhibited similar trends to oxidative stress-inducing enzymes under both normoxic and hypoxic conditions and triggered cell autophagy and ferroptosis. Administration of the ferroptosis inhibitor BRD4770 under normoxic conditions reversed temoporfin-based PDT-induced reductions in glutathione peroxidase 4 (GPx4), increasing in light chain 3-II (LC3-II) and cleaved poly (ADP-ribose) polymerase (cleaved-PARP). This study confirms that hypoxia weakens the anticancer effects of temoporfin-based PDT and that ferroptosis plays a key role in temoporfin-based PDT-mediated cancer cell inhibition.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"267 ","pages":"Article 113165"},"PeriodicalIF":3.9,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143855086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid penetrating inactivation and disinfection through ice layers by light-condensed deep ultraviolet LED irradiation","authors":"Lucheng Yu ,&nbsp;Peng Shen ,&nbsp;Zefeng Lin ,&nbsp;Shini Yang ,&nbsp;Maowei Yang ,&nbsp;Yaxiao Lou ,&nbsp;Shiqiang Lu ,&nbsp;Feiya Xu ,&nbsp;Shengrong Huang ,&nbsp;Xueqiong Sun ,&nbsp;Jiamin He ,&nbsp;Huachun Wang ,&nbsp;Yanhua Zhang ,&nbsp;Canming Zhang ,&nbsp;Fawen Su ,&nbsp;Fuchun Xu ,&nbsp;Xiaohong Chen ,&nbsp;Ling Li ,&nbsp;Duanjun Cai ,&nbsp;Junyong Kang","doi":"10.1016/j.jphotobiol.2025.113154","DOIUrl":"10.1016/j.jphotobiol.2025.113154","url":null,"abstract":"<div><div>The longer survival of virus and bacteria under low temperature greatly accelerates the infection risk and duration. How to effectively and rapidly inactivate them beneath ice layer in cold chain logistics has been an important issue never being well studied. Here, we demonstrate a novel light condenser design of high power UVC-LED device for rapid penetrating inactivation through ice layers. With fish-scale, ellipsoidal and alumina coated condenser, a portable UVC (275 nm) LED device in ultrahigh light output power (&gt; 3 W) was fabricated in a narrowed propagation angle (&lt; 60^o). Such condensed light beams are able to overcome the interfacial refringence at the ice and bubble interfaces in different types of ice layers for enhancing the transmission rate. As a result, the penetrating UVC light could give rise to the effective necrosis and apoptosis influence as well as DNA/RNA destruction on cells. A 99.99 % inactivation rate of <em>Escherichia coli</em> and <em>Staphylococcus aureus</em> has been obtained through 5-mm-thickness opaque ice layer. By 1.5 s irradiation, SARS-COV-2 virus can be completely inactivated and disinfected. This work proved the strong power of UVC-LED for cold chain disinfection applications.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"267 ","pages":"Article 113154"},"PeriodicalIF":3.9,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143843254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of 254 nm ultraviolet C promoting ethanol-induced gastric injury healing - A potential physical factor therapy","authors":"Xiao Zhang ,&nbsp;Dong-mei Li ,&nbsp;Hong-long Chen ,&nbsp;Xi-sheng Lin ,&nbsp;Su-su Li ,&nbsp;Le Yu ,&nbsp;Nan Peng ,&nbsp;Li-kang Wang ,&nbsp;Na Wang ,&nbsp;Yue-ming Gao","doi":"10.1016/j.jphotobiol.2025.113164","DOIUrl":"10.1016/j.jphotobiol.2025.113164","url":null,"abstract":"<div><h3>Background</h3><div>Gastritis, gastric ulcer, and gastric bleeding are associated with gastric mucosal injury. Chemical drugs are the current mainstream method, but the limitations are also increasingly apparent. Accumulative basic and clinical evidence has confirmed that ultraviolet C (UVC) has a significant effect on improving superficial infectious inflammation, promoting the healing of injured wounds, mucous membranes and ulcers, and killing microorganisms. It is possible to explore a new treatment method by introducing UVC light into the stomach to treat the damaged gastric mucosa.</div></div><div><h3>Objective</h3><div>This study evaluated the efficacy of introducing 254 nm UVC into the stomach by the self-made light guide at different doses and time to promote the healing of injured gastric mucosa, and explored the potential mechanism underling the treatment effect.</div></div><div><h3>Methods</h3><div>The rat model of gastric mucosal injury induced by ethanol was intervened by UVC with different radiation doses. The ulcer index, histopathology, CAT, SOD, MDA, NO, TNF-α, IL-6 and EGF in gastric mucosa and serum were evaluated. In addition, the expression of ASC, IL-1β and Caspase-1 in gastric tissue was evaluated.</div></div><div><h3>Results</h3><div>UVC significantly improved the histopathological condition of damaged gastric mucosa and reduce the ulcer index. UVC reduced the levels of TNF-α and IL-6, increase the level of EGF in serum. It also significantly increased the levels of SOD, CAT and NO, and reduced the levels of MDA in serum and gastric tissue. The expression of ASC, IL-1β and Caspase-1 in gastric mucosa was significantly inhibited, and it showed a significant dose-dependent trend with UVC irradiation dose.</div></div><div><h3>Conclusion</h3><div>Intragastric irradiation of 254 nm UVC can effectively promote the repair of damaged gastric mucosa by inhibiting inflammatory factors, activating antioxidant system and inhibiting the apoptosis-related proteins, which may provide a promising way for clinical application of physical factors in the treatment of gastric mucosal injury. However, whether the technology can be finally applied to clinical practice requires more rigorous safety and effectiveness studies.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"267 ","pages":"Article 113164"},"PeriodicalIF":3.9,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Collagen type III resists UVB induced photoaging of HaCaT cells by regulating mTOR signaling pathway","authors":"Helei Liu ,&nbsp;Hao Li ,&nbsp;Pengwei Zhao ,&nbsp;Rina Du ,&nbsp;Yaoxing Gao","doi":"10.1016/j.jphotobiol.2025.113153","DOIUrl":"10.1016/j.jphotobiol.2025.113153","url":null,"abstract":"<div><h3>Background</h3><div>Ultraviolet (UV) is the main factor leading to skin photoaging. In the process of photoaging, the dynamic balance of extracellular matrix is broken, resulting in the increase of reactive oxygen species (ROS), the accumulation of matrix metalloproteinases (MMPs), the decrease of collagen synthesis and the increase of degradation, and the skin becomes loose and wrinkled and pigmented, eventually leading to skin aging.</div></div><div><h3>Objective</h3><div>To investigate the effect of collagen type III (COLIII) on the autophagy level of human immortalized keratinocytes (HaCaT) induced by ultraviolet B (UVB) and explore whether COLIII can inhibit skin photoaging by changing the autophagy level.</div></div><div><h3>Methods</h3><div>HaCaT cells were irradiated with UVB to establish a photoaging model, and then the cell migration and repair ability, oxidative stress and inflammation levels, and autophagy levels were detected to explore the effect and mechanism of COLIII on autophagy in photoaged HaCaT cells.</div></div><div><h3>Results</h3><div>UVB radiation inhibited the cell proliferation and migration repair ability, resulting in the increase of ROS, inflammatory factors IL-6 and MMP-1 in HaCaT cells, while COLIII could attenuate the phototoxicity caused by UVB radiation and resist photoaging. In addition, COLIII can affect the level of autophagy through mTOR signaling pathway and protect HaCaT cells from UVB induced cytotoxicity, thereby attenuating light damage.</div></div><div><h3>Conclusion</h3><div>COLIII inhibits the autophagy level of UVB irradiated HaCaT cells through mTOR pathway, so as to combat UVB induced skin photoaging.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"267 ","pages":"Article 113153"},"PeriodicalIF":3.9,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}