Journal of photochemistry and photobiology. B, Biology最新文献

{"title":"Therapeutic potential of wavelength-dependent photobiomodulation on gut inflammation in an in vitro intestinal model","authors":"Myungji Kang ,&nbsp;Jihye Jo ,&nbsp;Hwarang Shin ,&nbsp;Hyun Wook Kang","doi":"10.1016/j.jphotobiol.2025.113201","DOIUrl":"10.1016/j.jphotobiol.2025.113201","url":null,"abstract":"<div><div>Recently, photobiomodulation (PBM) has emerged as a novel therapeutic approach for modulating the gut microbiome, offering potential for the regulation of intestinal inflammation. Although PBM has been primarily used for various clinical applications, recent studies suggest that its effects may extend to the regulation of microbial imbalances and chronic inflammation. The biological effects of PBM are wavelength-dependent, as the wavelength of light determines tissue penetration depth and cellular response. The current study aimed to compare the inflammation-modulatory effects of PBM at four different wavelengths (405, 532, 635, and 808 nm) and to elucidate the underlying molecular mechanisms of PBM in intestinal inflammation. An <em>in vitro</em> co-culture model consisting of Caco-2 cells and <em>Lactobacillus</em> was established to simulate the intestinal environment. Cellular inflammation was induced by lipopolysaccharide (LPS) stimulation, followed by wavelength-dependent PBM treatment at a dosage of 10 J/cm² (100 mW/cm² for 100 s, applied as a single irradiation). Among the wavelengths, 635 nm significantly reduced nitric oxide production and suppressed the expression of pro-inflammatory cytokines (TNF-α, IL-6, IL-1β, and iNOS). Furthermore, western blot and qPCR analyses revealed that 635 nm PBM downregulated key signaling factors in the MAPK/NF-kB pathway, indicating a potential molecular mechanism for its anti-inflammatory effect. These findings suggest that PBM, particularly at 635 nm, may serve as an effective strategy for modulating intestinal inflammation. Further studies will investigate the anti-inflammation and microbiome modulation effects of PBM in an <em>in vivo</em> model of inflammatory bowel disease.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"269 ","pages":"Article 113201"},"PeriodicalIF":3.9,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144329660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infrared-A (IR-A) modulates the p53 pathway and diurnal clock in human skin and subcutaneous adipose tissue in vivo","authors":"Iina-Maria Häggqvist ,&nbsp;Juha Jernman ,&nbsp;Erna Snellman ,&nbsp;Petri Kärhä ,&nbsp;Rafael Pasternack ,&nbsp;Timo Partonen ,&nbsp;Piia Karisola","doi":"10.1016/j.jphotobiol.2025.113199","DOIUrl":"10.1016/j.jphotobiol.2025.113199","url":null,"abstract":"<div><div>Infrared-A (IR-A) radiation is thought to protect the skin from damage due to ultraviolet radiation and alter its carcinogenic potential, possibly by preventing keratinocyte apoptosis via p53 pathway modulation. Solar radiation, including IR-A, is a major factor in contributing to the entrainment of circadian rhythms. We examined the impacts of IR-A on the intrinsic clock and transcriptomics in individuals with different diurnal preference to understand the mechanisms by which IR-A acts in the human skin and subcutaneous adipose tissue. IR-A caused multiple gene expressional changes, mainly as immediate stress responses in 15 min, that were reversed within 24 h. IR-A irradiation increased the skin surface temperature (mean peak temperature 42.9 °C). In skin, the zinc finger proteins <em>ZNF490</em> and <em>ZNHIT2</em> correlated negatively with the maximum skin surface temperature. In adipose tissue, <em>CDKN1A</em>, which codes p21 protein, had a negative correlation with the skin surface temperature change. In the skin immunohistochemical staining, the circadian regulators CRY1 and CRY2 increased significantly 24 h after the irradiation, CRY1 already in 15 min. According to the diurnal preferences, morning-type individuals develop mostly innate immune responses, whereas evening-type of individuals had more pronounced responses through p53 pathway modulation, apoptosis, and autophagy.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"269 ","pages":"Article 113199"},"PeriodicalIF":3.9,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144229584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydroxyl-tunable type I photosensitizer based on curcumin for photodynamic therapy against hypoxic tumor cells","authors":"Yanping Wang ,&nbsp;Tong Mu ,&nbsp;Xuewei Li ,&nbsp;Xiuli Zheng ,&nbsp;Jie Sha ,&nbsp;Zhe Yu ,&nbsp;Yuanyuan Qin ,&nbsp;Haohui Ren ,&nbsp;Ying Wang ,&nbsp;Weimin Liu ,&nbsp;Pengfei Wang","doi":"10.1016/j.jphotobiol.2025.113189","DOIUrl":"10.1016/j.jphotobiol.2025.113189","url":null,"abstract":"<div><div>The development of effective approaches to design type-I photosensitizers is of great importance due to their less oxygen content dependency. In this work, we report a simple strategy for the preparation of type-I photosensitizer (CNOH) by introducing two hydroxyl groups into the type-II photosensitizer (CN). Hydroxyl is not only an electron-donating group but can also form hydrogen bonds, affecting the energy levels and polarity of the photosensitizer. The results indicated that the small ΔE_S1-T1 and substantial reduction potential of CNOH might facilitate electron transfer to generate superoxide anions through type I process. Additionally, compared with the dual organelle-targeted photosensitizer CN, CNOH only exhibited endoplasmic reticulum targeting due to the weaker lipophilicity of CNOH. Under hypoxic conditions, CNOH showed stronger phototoxicity to tumor cells than CN, making it more suitable for PDT in hypoxic tumors. This provides a new direction for the design of type I photosensitizers.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"269 ","pages":"Article 113189"},"PeriodicalIF":3.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144213063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fiber-optic-guided near-infrared laser exposure induces depolarization of cultured primary sensory neurons and modifies biophysical properties of human Nav1.5 channels","authors":"Florian Armăşescu ,&nbsp;Bogdan Amuzescu ,&nbsp;Roxana-Olimpia Gheorghe ,&nbsp;Mihail Ghenghea ,&nbsp;Violeta Ristoiu ,&nbsp;Jean Ciurea ,&nbsp;Ion Gruia","doi":"10.1016/j.jphotobiol.2025.113191","DOIUrl":"10.1016/j.jphotobiol.2025.113191","url":null,"abstract":"<div><div>Photobiomodulation, a therapeutic method promoting wound healing, reduction in inflammation, pain and apoptosis, was widely tested in neurological/psychiatric disorders. In Parkinson's disease positive results have been obtained recently by transcranial or deep-fiber-optic-based near-infrared (NIR) light application. We assessed the effects of NIR stimulation with a 808.5 nm diode laser applied via a multimode fiber with a sharp tip placed over the cell on enzyme-dissociated cultured adult rat primary sensory neurons and human embryo kidney (HEK293) cells stably expressing human voltage-dependent Na⁺ channels (Nav1.5) approached via patch-clamp. For each type of cell, specific series of voltage- or current-clamp protocols were applied initially and after 3 min of laser exposure or control conditions. Laser exposure induced in neurons a resting potential depolarization (6.6 ± 1.8 mV vs. 2.4 ± 1.8 mV in control, mean ± SEM, <em>p</em> = 0.0594). In Nav1.5-expressing cells, peak <em>I</em>_Na amplitude slightly increased after laser application (111.2 ± 14.9 % vs. 70.6 ± 10.4 % in control experiments), and in outside-out patches the differences were larger (96.64 ± 5.25 %-laser vs. 37.95 ± 9.14 %-control). Via chemiluminometry we evidenced a delayed increase in ATP production in laser-exposed HEK293 cells. An explanation of these effects is that NIR exposure facilitates ATP production, maintaining an adequate state of Na⁺ channels phosphorylation, but we cannot exclude direct polarization effects on macromolecules including ion channels produced by the intense oriented electric field of the laser beam.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"269 ","pages":"Article 113191"},"PeriodicalIF":3.9,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144195932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling antioxidant and tyrosinase inhibitory activities of ESIPT-based flavonoids: DFT/TD-DFT calculations and molecular docking","authors":"Xingzhu Tang ,&nbsp;Chaofan Sun ,&nbsp;Xin Tian ,&nbsp;Yulei Zhang ,&nbsp;Lingling Wang","doi":"10.1016/j.jphotobiol.2025.113190","DOIUrl":"10.1016/j.jphotobiol.2025.113190","url":null,"abstract":"<div><div>The antioxidant and tyrosinase inhibitory activities of excited-state flavonoids (fisetin and luteolin) with excited state intramolecular proton transfer process (ESIPT) property have been explored by quantum chemical methods and molecular docking. Combined with transition-state theory, the thermodynamic parameter calculations indicate that fisetin and luteolin scavenge hydrogen peroxide radical (HOO<img>) through hydrogen atom abstraction (HAA) and single electron transfer (SET) mechanisms, respectively, where the reaction energy barriers and rates based on the HAA mechanism are shown to indicate phenolic hydroxyl groups on the B-ring are more susceptible to HOO<img> attack than that on the A-ring. Potential energy curves show energy-barrier and ultrafast ESIPT processes for fisetin and luteolin, respectively, thus determining excited-state structures. Further, utilizing frontier molecular orbitals and averaged local ionization energy, the antioxidant activity of excited state molecules is explored, which reveals that the activity is significantly boosted upon photoexcitation, especially the keto^⁎ form formed after ESIPT process. Additionally, the molecular docking indicates that the flavonoids achieve inhibition of tyrosinase. The relationship between the ESIPT process and bioactivity of excited-state flavonoids not only provides a theoretical basis for the design of natural antioxidant-active molecules, but also develops a novel pathway for the exploitation of anti-aging agents based on photoactivation mechanisms.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"268 ","pages":"Article 113190"},"PeriodicalIF":3.9,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144184954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quaternized phthalocyanines as a tool against melanoma and a broad spectrum of bacteria and fungi","authors":"Daniel Ziental ,&nbsp;Eduardo Anaya-Plaza ,&nbsp;Patrycja Talarska-Kulczyk ,&nbsp;Agata Kubicka ,&nbsp;Jakub Żurawski ,&nbsp;Jolanta Dlugaszewska ,&nbsp;Andrés de la Escosura ,&nbsp;Tomas Torres ,&nbsp;Lukasz Sobotta","doi":"10.1016/j.jphotobiol.2025.113187","DOIUrl":"10.1016/j.jphotobiol.2025.113187","url":null,"abstract":"<div><div>Photodynamic therapy can be a powerful tool to combat two of the greatest challenges of 21st-century medicine – antibiotic-resistant infections and cancer. Each of these factors claims millions of human lives annually. In the presented study, a wide variety of cationic phthalocyanines was tested against selected pathogens: <em>Staphylococcus aureus</em>, methicillin-resistant <em>Staphylococcus aureus</em>, <em>Enterococcus faecalis</em>, <em>Escherichia coli</em>, <em>Escherichia coli</em> producing extended-spectrum β-lactamases, <em>Pseudomonas aeruginosa</em>, carbapenem resistant <em>Pseudomonas aeruginosa</em>, <em>Trichophyton mentagrophytes</em>, <em>Candida albicans</em>. The compounds used in this study, which are functionalized with pyridyloxy substituents and in some cases bear bulky substituents (<em>tert</em>-butylphenyl or pyrenyl moieties) at the 5th position of the pyridyloxy ring, proved to be remarkably effective against all pathogens, allowing for a reduction in the number of microorganisms between 1.4 and 5.6 logs. Additionally, coaction with the commonly used antibiotic – doxycycline – was achieved in in vitro studies. The effectiveness of these macrocycles was also evaluated against melanoma cells (MICH2), showing a significant reduction in melanoma cell viability up to 85 %. Therefore, the investigated compounds can be considered as promising photosensitizers in modern photodynamic therapy.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"268 ","pages":"Article 113187"},"PeriodicalIF":3.9,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144184953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Paeoniflorin protected UVA-exposed skin fibroblasts from caspase-3/GSDME mediated pyroptosis","authors":"Dongxin Shi ,&nbsp;Haitao Chu ,&nbsp;Yijun Sun ,&nbsp;Hailun He ,&nbsp;Wenyue Huang ,&nbsp;Hua Pan ,&nbsp;Cong Ma ,&nbsp;Shulan Yao ,&nbsp;Meihui Shi ,&nbsp;Hexiao Wang ,&nbsp;Yan Wu","doi":"10.1016/j.jphotobiol.2025.113188","DOIUrl":"10.1016/j.jphotobiol.2025.113188","url":null,"abstract":"<div><h3>Background</h3><div>UVA radiation can impact fibroblasts and leads to alterations in dermal connective tissue. Pyroptosis is a form of regulated cell death characterized by gasdermin-mediated membrane pore formation and release of intracellular contents. Whether UVA exposure can trigger pyroptosis in fibroblasts is unclear. Paeoniflorin has antioxidant properties, but whether it alleviates pyroptosis through inhibiting oxidative stress in fibroblasts is not yet determined.</div></div><div><h3>Objectives</h3><div>To identify the occurrence and the molecular mechanism of cell pyroptosis in skin fibroblasts, and explore the anti-pyroptotic effect of paeoniflorin in UVA induced photodamage protection.</div></div><div><h3>Methods</h3><div>Using Human skin fibroblasts (HSFs), wild-type primary human dermal fibroblasts (HDFs) and CASP3 knockdown HDFs, we analyzed the occurrence of pyroptosis by examining changes in morphology, LDH release and the expression of pyroptotic molecules after UVA radiation. UVA-exposed C57BL/6 mice and human sun-exposed skin samples were used to analyze the expression of caspase-3/GSDME. The role of oxidative stress was investigated by using NAC. Paeoniflorin treatment was analyzed for its effects on pyroptosis and the expression of caspase-3/GSDME.</div></div><div><h3>Results</h3><div>Both HSFs and HDFs exposed to UVA exhibited dose-dependent pyroptosis. In HDFs, UVA increased the expression of caspase-3, GSDME, PARP, caspase-9, Cytochrome C, Bax/Bcl-2, while the expression of NLRP3, caspase-1, caspase-4, GSDMD was not significantly changed. UVA-exposed C57BL/6 mice and human sun-exposed skin samples showed increased caspase-3/GSDME. Treatment of NAC or paeoniflorin suppressed UVA-induced pyroptosis and mitochondrial-mediated apoptosis.</div></div><div><h3>Conclusions</h3><div>UVA induced caspase-3/GSDME mediated pyroptosis in both HSFs and HDFs, and paeoniflorin protected against UVA induced photodamage by suppressing this pathway.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"269 ","pages":"Article 113188"},"PeriodicalIF":3.9,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144196012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RBC membrane-coated sorafenib-MXene-Au nanocomposites for synergistic chemo-photothermal therapy of liver cancer","authors":"Shehab Elbeltagi ,&nbsp;Mohammed Al-zharani ,&nbsp;Zienab E. Eldin","doi":"10.1016/j.jphotobiol.2025.113185","DOIUrl":"10.1016/j.jphotobiol.2025.113185","url":null,"abstract":"<div><div>Red blood cell (RBC) membrane has emerged as innovative biological nanocarriers. In this study, RBCs membrane-coated sorafenib (SF)-MXene-Au nanocomposite (SF-MX-Au@RBCs), was developed as a smart drug delivery (SDD) system, offering enhanced photothermal therapy (PTT) under near-infrared (NIR) irradiation. The synthesized SF-MX-Au@RBCs exhibited an average size of approximately 65 nm and a zeta potential of −22.11 mV. The cumulative SF release from SF-MX-Au@RBCs reached 61.4 % under NIR irradiation at pH 7.4 over 96 h. Furthermore, the treatment effectiveness of SF-MX-Au@RBCs as a chemo-PTT treatment was evaluated against HepG2 liver cancer cells. In vitro assay demonstrated significant cytotoxicity, with chemo-PTT achieving an IC₅₀ value of 7.3 μg/mL and leading to necrosis rates of 31.9 % while the total apoptosis rates was 56.3 % (29.5 % late and 26.8 % early apoptosis) in treated cells. Western blot analysis indicated significant suppression of phosphorylated ERK (p-ERK) and MEK (p-MEK) in the MEK/ERK signaling pathway, with greater inhibition observed in the SF-MX-Au@RBCs group compared to SF alone. Additionally, key angiogenesis-related proteins, involving VEGFR2, VEGFR3, and PDGFR, were downregulated, highlighting the superior antiangiogenic effects of the nanocomposite. In vivo studies utilizing a xenograft model in BALB/c mice under NIR revealed that the chemo-PTT treatment indicated the smallest tumor volume (140 mm³) and tumor weight (0.16 g) compared to the other treatment groups. The chemo-PTT approach significantly enhanced antitumor efficacy, highlighting the potential for further optimization and improved treatment outcomes through targeted drug delivery systems (DDS). In addition, a comprehensive molecular docking analysis was conducted to examine the binding interactions of SF and MXene with three crucial proteins, namely: RAF proto-oncogene serine/threonine-protein kinase, FGR tyrosine-protein kinase, and mitogen-activated protein kinase (MAPK). MXene demonstrated superior binding affinities across the investigated target proteins, with ΔG values ranging from −11.94 to −12.56 kcal/mol.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"268 ","pages":"Article 113185"},"PeriodicalIF":3.9,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144166554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-color imaging of lipid droplets and lysosomes with triphenylamine-phenyl silicon phthalocyanine nanoprobes for enhanced photodynamic therapy","authors":"Xiuqin Chen ,&nbsp;Shiqing Dong ,&nbsp;Jianling Chen ,&nbsp;Qiuhao Ye ,&nbsp;Sitong Cao ,&nbsp;Kun Wang ,&nbsp;Yiru Peng","doi":"10.1016/j.jphotobiol.2025.113186","DOIUrl":"10.1016/j.jphotobiol.2025.113186","url":null,"abstract":"<div><div>Lipid droplets (LDs), vital in cancer cell metabolism, are prevalent in malignant tumors. Elucidating their interaction with lysosomes is essential for understanding their metabolic functions. However, simultaneous dual-color imaging and photodynamic function targeting both LDs and lysosomes using fluorescent probes remain unexplored. Our study fills this gap with DSPE@TPA-SiPc, a biocompatible nanoparticle that penetrates cells and targets these organelles. Using two-photon imaging, we monitored DSPE@TPA-SiPc in breast cancer cells, noting its plasma membrane dissociation and rapid transport to lysosomes and LDs. DSPE@TPA-SiPc uniquely labeled LDs with blue fluorescence and lysosomes with red, responding to intracellular polarity. Additionally, DSPE@TPA-SiPc induced reactive oxygen species (ROS) in cells, showing enhanced phototoxicity against MDA-MB-231 cells with larger LDs than MCF-7 cells. This research introduces a novel dual-labeled probe for organelle imaging and a promising strategy for targeted cancer therapy.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"268 ","pages":"Article 113186"},"PeriodicalIF":3.9,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An oxygen-independent therapeutic nanosystem for fighting against hypoxic and antioxidant microenvironment of tumor","authors":"Zixuan Wang ,&nbsp;Shuwei Nie ,&nbsp;Manru Wang ,&nbsp;Huina Niu ,&nbsp;Liqi Wei ,&nbsp;Zhiqi Yang ,&nbsp;Xin Liu ,&nbsp;Yining Chen ,&nbsp;Yunan Yang ,&nbsp;Chunjiang Li ,&nbsp;Qin Zhang ,&nbsp;Lina Feng ,&nbsp;Hongxia Ma ,&nbsp;Rui Chen ,&nbsp;Yan Cheng","doi":"10.1016/j.jphotobiol.2025.113184","DOIUrl":"10.1016/j.jphotobiol.2025.113184","url":null,"abstract":"<div><div>The innate hypoxic and antioxidant defendant microenvironment are the main obstacles for improving reactive oxygen species (ROS) based therapeutic efficacy against cancer. Herein, bismuth tungstate (BWO) nanoparticles (NPs) were fabricated for ultrasound activated hydroxyl radical (OH•) production through being reacted with water in an oxygen-independent manner due to their more positive potential of valent band position than that of OH• generation. For relieving antioxidant defense, BWOST NPs were designed through loading L-buthionine sulfoximine into hollow BWO NPs to inhibit the synthesis of glutathione (GSH), and coating pH-responsive tannic acid-Fe complex on the surface to prevent drug leakage. Both <em>in vitro</em> and <em>in vivo</em> assessments demonstrated that BWOST NPs could effectively lower intracellular GSH levels, inducing apoptosis of cancer cells and eliminating tumors. Therefore, BWOST NPs showed an amplified sonodynamic therapy efficacy through lower antioxidant defense and oxygen-independent OH• generation, which provided an effective strategy for improving ROS based cancer therapy.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"268 ","pages":"Article 113184"},"PeriodicalIF":3.9,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}