Journal of photochemistry and photobiology. B, Biology最新文献

{"title":"Fiber-optic-guided near-infrared laser exposure induces depolarization of cultured primary sensory neurons and modifies biophysical properties of human Nav1.5 channels","authors":"Florian Armăşescu ,&nbsp;Bogdan Amuzescu ,&nbsp;Roxana-Olimpia Gheorghe ,&nbsp;Mihail Ghenghea ,&nbsp;Violeta Ristoiu ,&nbsp;Jean Ciurea ,&nbsp;Ion Gruia","doi":"10.1016/j.jphotobiol.2025.113191","DOIUrl":"10.1016/j.jphotobiol.2025.113191","url":null,"abstract":"<div><div>Photobiomodulation, a therapeutic method promoting wound healing, reduction in inflammation, pain and apoptosis, was widely tested in neurological/psychiatric disorders. In Parkinson's disease positive results have been obtained recently by transcranial or deep-fiber-optic-based near-infrared (NIR) light application. We assessed the effects of NIR stimulation with a 808.5 nm diode laser applied via a multimode fiber with a sharp tip placed over the cell on enzyme-dissociated cultured adult rat primary sensory neurons and human embryo kidney (HEK293) cells stably expressing human voltage-dependent Na⁺ channels (Nav1.5) approached via patch-clamp. For each type of cell, specific series of voltage- or current-clamp protocols were applied initially and after 3 min of laser exposure or control conditions. Laser exposure induced in neurons a resting potential depolarization (6.6 ± 1.8 mV vs. 2.4 ± 1.8 mV in control, mean ± SEM, <em>p</em> = 0.0594). In Nav1.5-expressing cells, peak <em>I</em>_Na amplitude slightly increased after laser application (111.2 ± 14.9 % vs. 70.6 ± 10.4 % in control experiments), and in outside-out patches the differences were larger (96.64 ± 5.25 %-laser vs. 37.95 ± 9.14 %-control). Via chemiluminometry we evidenced a delayed increase in ATP production in laser-exposed HEK293 cells. An explanation of these effects is that NIR exposure facilitates ATP production, maintaining an adequate state of Na⁺ channels phosphorylation, but we cannot exclude direct polarization effects on macromolecules including ion channels produced by the intense oriented electric field of the laser beam.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"269 ","pages":"Article 113191"},"PeriodicalIF":3.9,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144195932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling antioxidant and tyrosinase inhibitory activities of ESIPT-based flavonoids: DFT/TD-DFT calculations and molecular docking","authors":"Xingzhu Tang ,&nbsp;Chaofan Sun ,&nbsp;Xin Tian ,&nbsp;Yulei Zhang ,&nbsp;Lingling Wang","doi":"10.1016/j.jphotobiol.2025.113190","DOIUrl":"10.1016/j.jphotobiol.2025.113190","url":null,"abstract":"<div><div>The antioxidant and tyrosinase inhibitory activities of excited-state flavonoids (fisetin and luteolin) with excited state intramolecular proton transfer process (ESIPT) property have been explored by quantum chemical methods and molecular docking. Combined with transition-state theory, the thermodynamic parameter calculations indicate that fisetin and luteolin scavenge hydrogen peroxide radical (HOO<img>) through hydrogen atom abstraction (HAA) and single electron transfer (SET) mechanisms, respectively, where the reaction energy barriers and rates based on the HAA mechanism are shown to indicate phenolic hydroxyl groups on the B-ring are more susceptible to HOO<img> attack than that on the A-ring. Potential energy curves show energy-barrier and ultrafast ESIPT processes for fisetin and luteolin, respectively, thus determining excited-state structures. Further, utilizing frontier molecular orbitals and averaged local ionization energy, the antioxidant activity of excited state molecules is explored, which reveals that the activity is significantly boosted upon photoexcitation, especially the keto^⁎ form formed after ESIPT process. Additionally, the molecular docking indicates that the flavonoids achieve inhibition of tyrosinase. The relationship between the ESIPT process and bioactivity of excited-state flavonoids not only provides a theoretical basis for the design of natural antioxidant-active molecules, but also develops a novel pathway for the exploitation of anti-aging agents based on photoactivation mechanisms.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"268 ","pages":"Article 113190"},"PeriodicalIF":3.9,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144184954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quaternized phthalocyanines as a tool against melanoma and a broad spectrum of bacteria and fungi","authors":"Daniel Ziental ,&nbsp;Eduardo Anaya-Plaza ,&nbsp;Patrycja Talarska-Kulczyk ,&nbsp;Agata Kubicka ,&nbsp;Jakub Żurawski ,&nbsp;Jolanta Dlugaszewska ,&nbsp;Andrés de la Escosura ,&nbsp;Tomas Torres ,&nbsp;Lukasz Sobotta","doi":"10.1016/j.jphotobiol.2025.113187","DOIUrl":"10.1016/j.jphotobiol.2025.113187","url":null,"abstract":"<div><div>Photodynamic therapy can be a powerful tool to combat two of the greatest challenges of 21st-century medicine – antibiotic-resistant infections and cancer. Each of these factors claims millions of human lives annually. In the presented study, a wide variety of cationic phthalocyanines was tested against selected pathogens: <em>Staphylococcus aureus</em>, methicillin-resistant <em>Staphylococcus aureus</em>, <em>Enterococcus faecalis</em>, <em>Escherichia coli</em>, <em>Escherichia coli</em> producing extended-spectrum β-lactamases, <em>Pseudomonas aeruginosa</em>, carbapenem resistant <em>Pseudomonas aeruginosa</em>, <em>Trichophyton mentagrophytes</em>, <em>Candida albicans</em>. The compounds used in this study, which are functionalized with pyridyloxy substituents and in some cases bear bulky substituents (<em>tert</em>-butylphenyl or pyrenyl moieties) at the 5th position of the pyridyloxy ring, proved to be remarkably effective against all pathogens, allowing for a reduction in the number of microorganisms between 1.4 and 5.6 logs. Additionally, coaction with the commonly used antibiotic – doxycycline – was achieved in in vitro studies. The effectiveness of these macrocycles was also evaluated against melanoma cells (MICH2), showing a significant reduction in melanoma cell viability up to 85 %. Therefore, the investigated compounds can be considered as promising photosensitizers in modern photodynamic therapy.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"268 ","pages":"Article 113187"},"PeriodicalIF":3.9,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144184953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Paeoniflorin protected UVA-exposed skin fibroblasts from caspase-3/GSDME mediated pyroptosis","authors":"Dongxin Shi ,&nbsp;Haitao Chu ,&nbsp;Yijun Sun ,&nbsp;Hailun He ,&nbsp;Wenyue Huang ,&nbsp;Hua Pan ,&nbsp;Cong Ma ,&nbsp;Shulan Yao ,&nbsp;Meihui Shi ,&nbsp;Hexiao Wang ,&nbsp;Yan Wu","doi":"10.1016/j.jphotobiol.2025.113188","DOIUrl":"10.1016/j.jphotobiol.2025.113188","url":null,"abstract":"<div><h3>Background</h3><div>UVA radiation can impact fibroblasts and leads to alterations in dermal connective tissue. Pyroptosis is a form of regulated cell death characterized by gasdermin-mediated membrane pore formation and release of intracellular contents. Whether UVA exposure can trigger pyroptosis in fibroblasts is unclear. Paeoniflorin has antioxidant properties, but whether it alleviates pyroptosis through inhibiting oxidative stress in fibroblasts is not yet determined.</div></div><div><h3>Objectives</h3><div>To identify the occurrence and the molecular mechanism of cell pyroptosis in skin fibroblasts, and explore the anti-pyroptotic effect of paeoniflorin in UVA induced photodamage protection.</div></div><div><h3>Methods</h3><div>Using Human skin fibroblasts (HSFs), wild-type primary human dermal fibroblasts (HDFs) and CASP3 knockdown HDFs, we analyzed the occurrence of pyroptosis by examining changes in morphology, LDH release and the expression of pyroptotic molecules after UVA radiation. UVA-exposed C57BL/6 mice and human sun-exposed skin samples were used to analyze the expression of caspase-3/GSDME. The role of oxidative stress was investigated by using NAC. Paeoniflorin treatment was analyzed for its effects on pyroptosis and the expression of caspase-3/GSDME.</div></div><div><h3>Results</h3><div>Both HSFs and HDFs exposed to UVA exhibited dose-dependent pyroptosis. In HDFs, UVA increased the expression of caspase-3, GSDME, PARP, caspase-9, Cytochrome C, Bax/Bcl-2, while the expression of NLRP3, caspase-1, caspase-4, GSDMD was not significantly changed. UVA-exposed C57BL/6 mice and human sun-exposed skin samples showed increased caspase-3/GSDME. Treatment of NAC or paeoniflorin suppressed UVA-induced pyroptosis and mitochondrial-mediated apoptosis.</div></div><div><h3>Conclusions</h3><div>UVA induced caspase-3/GSDME mediated pyroptosis in both HSFs and HDFs, and paeoniflorin protected against UVA induced photodamage by suppressing this pathway.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"269 ","pages":"Article 113188"},"PeriodicalIF":3.9,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144196012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RBC membrane-coated sorafenib-MXene-Au nanocomposites for synergistic chemo-photothermal therapy of liver cancer","authors":"Shehab Elbeltagi ,&nbsp;Mohammed Al-zharani ,&nbsp;Zienab E. Eldin","doi":"10.1016/j.jphotobiol.2025.113185","DOIUrl":"10.1016/j.jphotobiol.2025.113185","url":null,"abstract":"<div><div>Red blood cell (RBC) membrane has emerged as innovative biological nanocarriers. In this study, RBCs membrane-coated sorafenib (SF)-MXene-Au nanocomposite (SF-MX-Au@RBCs), was developed as a smart drug delivery (SDD) system, offering enhanced photothermal therapy (PTT) under near-infrared (NIR) irradiation. The synthesized SF-MX-Au@RBCs exhibited an average size of approximately 65 nm and a zeta potential of −22.11 mV. The cumulative SF release from SF-MX-Au@RBCs reached 61.4 % under NIR irradiation at pH 7.4 over 96 h. Furthermore, the treatment effectiveness of SF-MX-Au@RBCs as a chemo-PTT treatment was evaluated against HepG2 liver cancer cells. In vitro assay demonstrated significant cytotoxicity, with chemo-PTT achieving an IC₅₀ value of 7.3 μg/mL and leading to necrosis rates of 31.9 % while the total apoptosis rates was 56.3 % (29.5 % late and 26.8 % early apoptosis) in treated cells. Western blot analysis indicated significant suppression of phosphorylated ERK (p-ERK) and MEK (p-MEK) in the MEK/ERK signaling pathway, with greater inhibition observed in the SF-MX-Au@RBCs group compared to SF alone. Additionally, key angiogenesis-related proteins, involving VEGFR2, VEGFR3, and PDGFR, were downregulated, highlighting the superior antiangiogenic effects of the nanocomposite. In vivo studies utilizing a xenograft model in BALB/c mice under NIR revealed that the chemo-PTT treatment indicated the smallest tumor volume (140 mm³) and tumor weight (0.16 g) compared to the other treatment groups. The chemo-PTT approach significantly enhanced antitumor efficacy, highlighting the potential for further optimization and improved treatment outcomes through targeted drug delivery systems (DDS). In addition, a comprehensive molecular docking analysis was conducted to examine the binding interactions of SF and MXene with three crucial proteins, namely: RAF proto-oncogene serine/threonine-protein kinase, FGR tyrosine-protein kinase, and mitogen-activated protein kinase (MAPK). MXene demonstrated superior binding affinities across the investigated target proteins, with ΔG values ranging from −11.94 to −12.56 kcal/mol.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"268 ","pages":"Article 113185"},"PeriodicalIF":3.9,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144166554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-color imaging of lipid droplets and lysosomes with triphenylamine-phenyl silicon phthalocyanine nanoprobes for enhanced photodynamic therapy","authors":"Xiuqin Chen ,&nbsp;Shiqing Dong ,&nbsp;Jianling Chen ,&nbsp;Qiuhao Ye ,&nbsp;Sitong Cao ,&nbsp;Kun Wang ,&nbsp;Yiru Peng","doi":"10.1016/j.jphotobiol.2025.113186","DOIUrl":"10.1016/j.jphotobiol.2025.113186","url":null,"abstract":"<div><div>Lipid droplets (LDs), vital in cancer cell metabolism, are prevalent in malignant tumors. Elucidating their interaction with lysosomes is essential for understanding their metabolic functions. However, simultaneous dual-color imaging and photodynamic function targeting both LDs and lysosomes using fluorescent probes remain unexplored. Our study fills this gap with DSPE@TPA-SiPc, a biocompatible nanoparticle that penetrates cells and targets these organelles. Using two-photon imaging, we monitored DSPE@TPA-SiPc in breast cancer cells, noting its plasma membrane dissociation and rapid transport to lysosomes and LDs. DSPE@TPA-SiPc uniquely labeled LDs with blue fluorescence and lysosomes with red, responding to intracellular polarity. Additionally, DSPE@TPA-SiPc induced reactive oxygen species (ROS) in cells, showing enhanced phototoxicity against MDA-MB-231 cells with larger LDs than MCF-7 cells. This research introduces a novel dual-labeled probe for organelle imaging and a promising strategy for targeted cancer therapy.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"268 ","pages":"Article 113186"},"PeriodicalIF":3.9,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An oxygen-independent therapeutic nanosystem for fighting against hypoxic and antioxidant microenvironment of tumor","authors":"Zixuan Wang ,&nbsp;Shuwei Nie ,&nbsp;Manru Wang ,&nbsp;Huina Niu ,&nbsp;Liqi Wei ,&nbsp;Zhiqi Yang ,&nbsp;Xin Liu ,&nbsp;Yining Chen ,&nbsp;Yunan Yang ,&nbsp;Chunjiang Li ,&nbsp;Qin Zhang ,&nbsp;Lina Feng ,&nbsp;Hongxia Ma ,&nbsp;Rui Chen ,&nbsp;Yan Cheng","doi":"10.1016/j.jphotobiol.2025.113184","DOIUrl":"10.1016/j.jphotobiol.2025.113184","url":null,"abstract":"<div><div>The innate hypoxic and antioxidant defendant microenvironment are the main obstacles for improving reactive oxygen species (ROS) based therapeutic efficacy against cancer. Herein, bismuth tungstate (BWO) nanoparticles (NPs) were fabricated for ultrasound activated hydroxyl radical (OH•) production through being reacted with water in an oxygen-independent manner due to their more positive potential of valent band position than that of OH• generation. For relieving antioxidant defense, BWOST NPs were designed through loading L-buthionine sulfoximine into hollow BWO NPs to inhibit the synthesis of glutathione (GSH), and coating pH-responsive tannic acid-Fe complex on the surface to prevent drug leakage. Both <em>in vitro</em> and <em>in vivo</em> assessments demonstrated that BWOST NPs could effectively lower intracellular GSH levels, inducing apoptosis of cancer cells and eliminating tumors. Therefore, BWOST NPs showed an amplified sonodynamic therapy efficacy through lower antioxidant defense and oxygen-independent OH• generation, which provided an effective strategy for improving ROS based cancer therapy.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"268 ","pages":"Article 113184"},"PeriodicalIF":3.9,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pretreatment of cancer cells with inhibitors of PKCδ, EGFR, and JNK increased intracellular hypericin content and enhanced the effectiveness of photodynamic therapy","authors":"Romanovová Michaela ,&nbsp;Jendželovská Zuzana ,&nbsp;Barčáková Ivana,&nbsp;Majerník Martin,&nbsp;Jendželovský Rastislav,&nbsp;Fedoročko Peter","doi":"10.1016/j.jphotobiol.2025.113183","DOIUrl":"10.1016/j.jphotobiol.2025.113183","url":null,"abstract":"<div><div>Photodynamic therapy (PDT) is an effective, minimally invasive treatment for certain cancers that uses photosensitizers (PSs) to selectively destroy tumor cells upon light activation. However, the breast cancer resistance protein (BCRP) plays a critical role in limiting PDT efficacy through active efflux of various PSs from the cancer cells. Hypericin (HY), a potent and promising natural PS, is also a preferential BCRP substrate, and its cytotoxic effect in PDT is reduced in BCRP-overexpressing cells. Thus, the main aim of our study was to investigate the potential of the modulators of signaling molecules possibly involved in BCRP regulation to sensitize cancer cells (A549, HT-29, RPMI-8226, and RPMI-8226/MR20) to hypericin-mediated PDT (HY-PDT). We assessed the effects of inhibitors of epidermal growth factor receptor (EGFR) (Tyr – tyrphostin AG 1478) and c-Jun N-terminal kinase (JNK) (SP – SP600125, Lico – licochalcone A), as well as protein kinase C (PKC) activator (TPA) and inhibitor (Rot – rottlerin). Our results showed that all modulators enhanced HY-PDT efficacy, but the extent of sensitization, as well as the specific effects, varied depending on the cell line. Pretreatment with Tyr, SP, and Lico significantly improved HY-PDT in RPMI-8226 and RPMI-8226/MR20 cells, while Rot pretreatment had the strongest effect in A549 and HT-29 cells. More importantly, the sensitizing effects of the inhibitors were linked to increased intracellular HY accumulation, indicating reduced BCRP efflux activity. While the exact mechanisms behind these effects require further investigation, our findings suggest that targeting BCRP and associated signaling pathways could enhance PDT outcomes in cancer treatment.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"268 ","pages":"Article 113183"},"PeriodicalIF":3.9,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144098783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of blue LED in enhancing hair treatments: Analysis in different hair ethnicities","authors":"Juliana Ferreira-Strixino ,&nbsp;Priscila Vareschi Cardoso Fujimoto ,&nbsp;Priscila Maria Sarmeiro Correa Marciano Leite ,&nbsp;Juliana Guerra Pinto","doi":"10.1016/j.jphotobiol.2025.113181","DOIUrl":"10.1016/j.jphotobiol.2025.113181","url":null,"abstract":"<div><div>Blue LED has several clinical applications, including enhancing the permeation of cosmetics into hair and enhancing hair treatments. This study evaluated its efficacy in these processes in different hair ethnicities, in addition to analyzing the changes in the physicochemical properties of the hair fiber after treatment. The study used virgin hair of Caucasian, African, and Asian types free from previous chemical treatments. Different application approaches with blue LED were adopted: spot application and sweeping application. For the analyses, six hair samples were prepared for each ethnic group, comprising: 1) Control without treatment; 2) Treated with leave-in cosmetic; 3) Treated with leave-in cosmetic and irradiated with blue LED in sweeping mode; 4) Treated with leave-in cosmetic and irradiated with blue LED in spot mode; 5) Untreated hair fiber irradiated with blue LED in sweeping mode; 6) Untreated hair fiber irradiated with blue LED in spot mode. Differences in cosmetic permeation into the hair strand after irradiation with blue LED were observed among different hair ethnicities. The Caucasian and Asian hair groups, when treated with cosmetics and irradiated with blue LED in the form of spot application and sweeping, showed a significant increase in cosmetic permeation, diameter, and resistance of the hair fiber compared to the African hair group.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"268 ","pages":"Article 113181"},"PeriodicalIF":3.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A wash-free red fluorescent probe for real-time monitoring of mitochondrial viscosity changes and tumor imaging","authors":"Kun Yu ,&nbsp;Yeming Zhang ,&nbsp;Yuqing Wang ,&nbsp;Jiale Li ,&nbsp;Jie Wang ,&nbsp;Sichen Zhang ,&nbsp;Aobo Sun ,&nbsp;Xue Zhang ,&nbsp;Lei Hu ,&nbsp;Hui Wang","doi":"10.1016/j.jphotobiol.2025.113182","DOIUrl":"10.1016/j.jphotobiol.2025.113182","url":null,"abstract":"<div><div>Mitochondria are important energy-producing organelles within cells, and abnormalities in the viscosity of their microenvironment are closely related to diseases such as cancer. Current methods for detecting viscosity still suffer from many limitations, whereas fluorescence imaging techniques can address these shortcomings. Therefore, there is an urgent need to develop fluorescence probes capable of detecting changes in mitochondrial viscosity. In this study, derivatives of triphenylamine were used as the core structure, and different triphenylphosphine derivatives were introduced through a condensation reaction to synthesize three red fluorescence probes with a D-π-A structure. Their photophysical properties have been systematically studied and it has been found that they are not only sensitive to viscosity but also exhibit strong anti-jamming capabilities. Among them, probe <strong>TPAP2</strong> exhibits excellent optical properties, including large Stokes shifts and high sensitivity to viscosity. It was found that <strong>TPAP2</strong> is mitochondria-targeted, washing-free, and fast (&lt;10s), with the targeting process depending on the mitochondrial membrane potential. The probe has been successfully applied to tumor imaging in mice with subcutaneous tumors.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"268 ","pages":"Article 113182"},"PeriodicalIF":3.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}