Journal of photochemistry and photobiology. B, Biology最新文献

{"title":"Paeoniflorin protected UVA-exposed skin fibroblasts from caspase-3/GSDME mediated pyroptosis","authors":"Dongxin Shi ,&nbsp;Haitao Chu ,&nbsp;Yijun Sun ,&nbsp;Hailun He ,&nbsp;Wenyue Huang ,&nbsp;Hua Pan ,&nbsp;Cong Ma ,&nbsp;Shulan Yao ,&nbsp;Meihui Shi ,&nbsp;Hexiao Wang ,&nbsp;Yan Wu","doi":"10.1016/j.jphotobiol.2025.113188","DOIUrl":"10.1016/j.jphotobiol.2025.113188","url":null,"abstract":"<div><h3>Background</h3><div>UVA radiation can impact fibroblasts and leads to alterations in dermal connective tissue. Pyroptosis is a form of regulated cell death characterized by gasdermin-mediated membrane pore formation and release of intracellular contents. Whether UVA exposure can trigger pyroptosis in fibroblasts is unclear. Paeoniflorin has antioxidant properties, but whether it alleviates pyroptosis through inhibiting oxidative stress in fibroblasts is not yet determined.</div></div><div><h3>Objectives</h3><div>To identify the occurrence and the molecular mechanism of cell pyroptosis in skin fibroblasts, and explore the anti-pyroptotic effect of paeoniflorin in UVA induced photodamage protection.</div></div><div><h3>Methods</h3><div>Using Human skin fibroblasts (HSFs), wild-type primary human dermal fibroblasts (HDFs) and CASP3 knockdown HDFs, we analyzed the occurrence of pyroptosis by examining changes in morphology, LDH release and the expression of pyroptotic molecules after UVA radiation. UVA-exposed C57BL/6 mice and human sun-exposed skin samples were used to analyze the expression of caspase-3/GSDME. The role of oxidative stress was investigated by using NAC. Paeoniflorin treatment was analyzed for its effects on pyroptosis and the expression of caspase-3/GSDME.</div></div><div><h3>Results</h3><div>Both HSFs and HDFs exposed to UVA exhibited dose-dependent pyroptosis. In HDFs, UVA increased the expression of caspase-3, GSDME, PARP, caspase-9, Cytochrome C, Bax/Bcl-2, while the expression of NLRP3, caspase-1, caspase-4, GSDMD was not significantly changed. UVA-exposed C57BL/6 mice and human sun-exposed skin samples showed increased caspase-3/GSDME. Treatment of NAC or paeoniflorin suppressed UVA-induced pyroptosis and mitochondrial-mediated apoptosis.</div></div><div><h3>Conclusions</h3><div>UVA induced caspase-3/GSDME mediated pyroptosis in both HSFs and HDFs, and paeoniflorin protected against UVA induced photodamage by suppressing this pathway.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"269 ","pages":"Article 113188"},"PeriodicalIF":3.9,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144196012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RBC membrane-coated sorafenib-MXene-Au nanocomposites for synergistic chemo-photothermal therapy of liver cancer","authors":"Shehab Elbeltagi ,&nbsp;Mohammed Al-zharani ,&nbsp;Zienab E. Eldin","doi":"10.1016/j.jphotobiol.2025.113185","DOIUrl":"10.1016/j.jphotobiol.2025.113185","url":null,"abstract":"<div><div>Red blood cell (RBC) membrane has emerged as innovative biological nanocarriers. In this study, RBCs membrane-coated sorafenib (SF)-MXene-Au nanocomposite (SF-MX-Au@RBCs), was developed as a smart drug delivery (SDD) system, offering enhanced photothermal therapy (PTT) under near-infrared (NIR) irradiation. The synthesized SF-MX-Au@RBCs exhibited an average size of approximately 65 nm and a zeta potential of −22.11 mV. The cumulative SF release from SF-MX-Au@RBCs reached 61.4 % under NIR irradiation at pH 7.4 over 96 h. Furthermore, the treatment effectiveness of SF-MX-Au@RBCs as a chemo-PTT treatment was evaluated against HepG2 liver cancer cells. In vitro assay demonstrated significant cytotoxicity, with chemo-PTT achieving an IC₅₀ value of 7.3 μg/mL and leading to necrosis rates of 31.9 % while the total apoptosis rates was 56.3 % (29.5 % late and 26.8 % early apoptosis) in treated cells. Western blot analysis indicated significant suppression of phosphorylated ERK (p-ERK) and MEK (p-MEK) in the MEK/ERK signaling pathway, with greater inhibition observed in the SF-MX-Au@RBCs group compared to SF alone. Additionally, key angiogenesis-related proteins, involving VEGFR2, VEGFR3, and PDGFR, were downregulated, highlighting the superior antiangiogenic effects of the nanocomposite. In vivo studies utilizing a xenograft model in BALB/c mice under NIR revealed that the chemo-PTT treatment indicated the smallest tumor volume (140 mm³) and tumor weight (0.16 g) compared to the other treatment groups. The chemo-PTT approach significantly enhanced antitumor efficacy, highlighting the potential for further optimization and improved treatment outcomes through targeted drug delivery systems (DDS). In addition, a comprehensive molecular docking analysis was conducted to examine the binding interactions of SF and MXene with three crucial proteins, namely: RAF proto-oncogene serine/threonine-protein kinase, FGR tyrosine-protein kinase, and mitogen-activated protein kinase (MAPK). MXene demonstrated superior binding affinities across the investigated target proteins, with ΔG values ranging from −11.94 to −12.56 kcal/mol.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"268 ","pages":"Article 113185"},"PeriodicalIF":3.9,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144166554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-color imaging of lipid droplets and lysosomes with triphenylamine-phenyl silicon phthalocyanine nanoprobes for enhanced photodynamic therapy","authors":"Xiuqin Chen ,&nbsp;Shiqing Dong ,&nbsp;Jianling Chen ,&nbsp;Qiuhao Ye ,&nbsp;Sitong Cao ,&nbsp;Kun Wang ,&nbsp;Yiru Peng","doi":"10.1016/j.jphotobiol.2025.113186","DOIUrl":"10.1016/j.jphotobiol.2025.113186","url":null,"abstract":"<div><div>Lipid droplets (LDs), vital in cancer cell metabolism, are prevalent in malignant tumors. Elucidating their interaction with lysosomes is essential for understanding their metabolic functions. However, simultaneous dual-color imaging and photodynamic function targeting both LDs and lysosomes using fluorescent probes remain unexplored. Our study fills this gap with DSPE@TPA-SiPc, a biocompatible nanoparticle that penetrates cells and targets these organelles. Using two-photon imaging, we monitored DSPE@TPA-SiPc in breast cancer cells, noting its plasma membrane dissociation and rapid transport to lysosomes and LDs. DSPE@TPA-SiPc uniquely labeled LDs with blue fluorescence and lysosomes with red, responding to intracellular polarity. Additionally, DSPE@TPA-SiPc induced reactive oxygen species (ROS) in cells, showing enhanced phototoxicity against MDA-MB-231 cells with larger LDs than MCF-7 cells. This research introduces a novel dual-labeled probe for organelle imaging and a promising strategy for targeted cancer therapy.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"268 ","pages":"Article 113186"},"PeriodicalIF":3.9,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An oxygen-independent therapeutic nanosystem for fighting against hypoxic and antioxidant microenvironment of tumor","authors":"Zixuan Wang ,&nbsp;Shuwei Nie ,&nbsp;Manru Wang ,&nbsp;Huina Niu ,&nbsp;Liqi Wei ,&nbsp;Zhiqi Yang ,&nbsp;Xin Liu ,&nbsp;Yining Chen ,&nbsp;Yunan Yang ,&nbsp;Chunjiang Li ,&nbsp;Qin Zhang ,&nbsp;Lina Feng ,&nbsp;Hongxia Ma ,&nbsp;Rui Chen ,&nbsp;Yan Cheng","doi":"10.1016/j.jphotobiol.2025.113184","DOIUrl":"10.1016/j.jphotobiol.2025.113184","url":null,"abstract":"<div><div>The innate hypoxic and antioxidant defendant microenvironment are the main obstacles for improving reactive oxygen species (ROS) based therapeutic efficacy against cancer. Herein, bismuth tungstate (BWO) nanoparticles (NPs) were fabricated for ultrasound activated hydroxyl radical (OH•) production through being reacted with water in an oxygen-independent manner due to their more positive potential of valent band position than that of OH• generation. For relieving antioxidant defense, BWOST NPs were designed through loading L-buthionine sulfoximine into hollow BWO NPs to inhibit the synthesis of glutathione (GSH), and coating pH-responsive tannic acid-Fe complex on the surface to prevent drug leakage. Both <em>in vitro</em> and <em>in vivo</em> assessments demonstrated that BWOST NPs could effectively lower intracellular GSH levels, inducing apoptosis of cancer cells and eliminating tumors. Therefore, BWOST NPs showed an amplified sonodynamic therapy efficacy through lower antioxidant defense and oxygen-independent OH• generation, which provided an effective strategy for improving ROS based cancer therapy.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"268 ","pages":"Article 113184"},"PeriodicalIF":3.9,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pretreatment of cancer cells with inhibitors of PKCδ, EGFR, and JNK increased intracellular hypericin content and enhanced the effectiveness of photodynamic therapy","authors":"Romanovová Michaela ,&nbsp;Jendželovská Zuzana ,&nbsp;Barčáková Ivana,&nbsp;Majerník Martin,&nbsp;Jendželovský Rastislav,&nbsp;Fedoročko Peter","doi":"10.1016/j.jphotobiol.2025.113183","DOIUrl":"10.1016/j.jphotobiol.2025.113183","url":null,"abstract":"<div><div>Photodynamic therapy (PDT) is an effective, minimally invasive treatment for certain cancers that uses photosensitizers (PSs) to selectively destroy tumor cells upon light activation. However, the breast cancer resistance protein (BCRP) plays a critical role in limiting PDT efficacy through active efflux of various PSs from the cancer cells. Hypericin (HY), a potent and promising natural PS, is also a preferential BCRP substrate, and its cytotoxic effect in PDT is reduced in BCRP-overexpressing cells. Thus, the main aim of our study was to investigate the potential of the modulators of signaling molecules possibly involved in BCRP regulation to sensitize cancer cells (A549, HT-29, RPMI-8226, and RPMI-8226/MR20) to hypericin-mediated PDT (HY-PDT). We assessed the effects of inhibitors of epidermal growth factor receptor (EGFR) (Tyr – tyrphostin AG 1478) and c-Jun N-terminal kinase (JNK) (SP – SP600125, Lico – licochalcone A), as well as protein kinase C (PKC) activator (TPA) and inhibitor (Rot – rottlerin). Our results showed that all modulators enhanced HY-PDT efficacy, but the extent of sensitization, as well as the specific effects, varied depending on the cell line. Pretreatment with Tyr, SP, and Lico significantly improved HY-PDT in RPMI-8226 and RPMI-8226/MR20 cells, while Rot pretreatment had the strongest effect in A549 and HT-29 cells. More importantly, the sensitizing effects of the inhibitors were linked to increased intracellular HY accumulation, indicating reduced BCRP efflux activity. While the exact mechanisms behind these effects require further investigation, our findings suggest that targeting BCRP and associated signaling pathways could enhance PDT outcomes in cancer treatment.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"268 ","pages":"Article 113183"},"PeriodicalIF":3.9,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144098783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of blue LED in enhancing hair treatments: Analysis in different hair ethnicities","authors":"Juliana Ferreira-Strixino ,&nbsp;Priscila Vareschi Cardoso Fujimoto ,&nbsp;Priscila Maria Sarmeiro Correa Marciano Leite ,&nbsp;Juliana Guerra Pinto","doi":"10.1016/j.jphotobiol.2025.113181","DOIUrl":"10.1016/j.jphotobiol.2025.113181","url":null,"abstract":"<div><div>Blue LED has several clinical applications, including enhancing the permeation of cosmetics into hair and enhancing hair treatments. This study evaluated its efficacy in these processes in different hair ethnicities, in addition to analyzing the changes in the physicochemical properties of the hair fiber after treatment. The study used virgin hair of Caucasian, African, and Asian types free from previous chemical treatments. Different application approaches with blue LED were adopted: spot application and sweeping application. For the analyses, six hair samples were prepared for each ethnic group, comprising: 1) Control without treatment; 2) Treated with leave-in cosmetic; 3) Treated with leave-in cosmetic and irradiated with blue LED in sweeping mode; 4) Treated with leave-in cosmetic and irradiated with blue LED in spot mode; 5) Untreated hair fiber irradiated with blue LED in sweeping mode; 6) Untreated hair fiber irradiated with blue LED in spot mode. Differences in cosmetic permeation into the hair strand after irradiation with blue LED were observed among different hair ethnicities. The Caucasian and Asian hair groups, when treated with cosmetics and irradiated with blue LED in the form of spot application and sweeping, showed a significant increase in cosmetic permeation, diameter, and resistance of the hair fiber compared to the African hair group.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"268 ","pages":"Article 113181"},"PeriodicalIF":3.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A wash-free red fluorescent probe for real-time monitoring of mitochondrial viscosity changes and tumor imaging","authors":"Kun Yu ,&nbsp;Yeming Zhang ,&nbsp;Yuqing Wang ,&nbsp;Jiale Li ,&nbsp;Jie Wang ,&nbsp;Sichen Zhang ,&nbsp;Aobo Sun ,&nbsp;Xue Zhang ,&nbsp;Lei Hu ,&nbsp;Hui Wang","doi":"10.1016/j.jphotobiol.2025.113182","DOIUrl":"10.1016/j.jphotobiol.2025.113182","url":null,"abstract":"<div><div>Mitochondria are important energy-producing organelles within cells, and abnormalities in the viscosity of their microenvironment are closely related to diseases such as cancer. Current methods for detecting viscosity still suffer from many limitations, whereas fluorescence imaging techniques can address these shortcomings. Therefore, there is an urgent need to develop fluorescence probes capable of detecting changes in mitochondrial viscosity. In this study, derivatives of triphenylamine were used as the core structure, and different triphenylphosphine derivatives were introduced through a condensation reaction to synthesize three red fluorescence probes with a D-π-A structure. Their photophysical properties have been systematically studied and it has been found that they are not only sensitive to viscosity but also exhibit strong anti-jamming capabilities. Among them, probe <strong>TPAP2</strong> exhibits excellent optical properties, including large Stokes shifts and high sensitivity to viscosity. It was found that <strong>TPAP2</strong> is mitochondria-targeted, washing-free, and fast (&lt;10s), with the targeting process depending on the mitochondrial membrane potential. The probe has been successfully applied to tumor imaging in mice with subcutaneous tumors.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"268 ","pages":"Article 113182"},"PeriodicalIF":3.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of various carbon supports for TiO2 and N-doped TiO2 photocatalysts for inactivation of Escherichia coli in water under UV–visible light","authors":"Mutcha Shanmukha Rao ,&nbsp;Ashish Tiwari ,&nbsp;Diptipriya Sethi ,&nbsp;Tapan Dash ,&nbsp;Kamatchi Jothiramalingam Sankaran ,&nbsp;Ramasamy Sakthivel","doi":"10.1016/j.jphotobiol.2025.113180","DOIUrl":"10.1016/j.jphotobiol.2025.113180","url":null,"abstract":"<div><div>This study evaluates the effectiveness of various carbon nanostructures carbon glassy spheres (CGS), carbon nanopowder (CNP), and activated carbon (AC) as supports for TiO₂ and N-TiO₂ photocatalysts in the inactivation of <em>Escherichia coli (E. coli)</em> in water. The photocatalysts were synthesized using a sol-gel method. Transmission electron microscopy (TEM) and X-ray diffraction (XRD) analyses confirmed the presence of anatase-phase TiO₂ on the carbon supports, with the synthesized TiO₂ nanoparticles exhibiting particle sizes below 10 nm. Fourier transform infrared (FTIR) spectroscopy identified metal‑oxygen bonds and surface hydroxyl groups on the photocatalyst surface, while X-ray photoelectron spectroscopy (XPS) confirmed the Ti⁴⁺ oxidation state and nitrogen incorporation in nitrogen-doped TiO₂ (N-TiO₂) samples. The photocatalytic performance of these materials was investigated for <em>E. coli</em> inactivation under both UV and UV–visible light irradiation. Results demonstrated that carbon-supported TiO₂ and N-TiO₂ nanocomposites exhibited significantly enhanced photocatalytic activity compared to their unsupported counterparts. Superior photocatalytic performance was observed under UV–visible light compared to UV light. Among the three carbon-supported systems, TiO₂ and N-TiO₂ nanocomposites deposited on CGS achieved the highest photocatalytic activity. The enhanced performance was attributed to the morphology and particle size of the carbon supports, which played critical roles in optimizing photocatalytic inactivation of <em>E. coli</em> in water.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"268 ","pages":"Article 113180"},"PeriodicalIF":3.9,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanobioactive glass/chitosan/collagen composite loaded with methylene blue for tissue regeneration and bacterial infection treatment by photodynamic therapy","authors":"Sarah M. Ali ,&nbsp;Elshimaa H. Gomaa ,&nbsp;Entsar E. Badr ,&nbsp;M.A. Azooz ,&nbsp;Mohammad M. Farag","doi":"10.1016/j.jphotobiol.2025.113179","DOIUrl":"10.1016/j.jphotobiol.2025.113179","url":null,"abstract":"<div><div>Development of suitable tissue engineering scaffolds to be loaded with photosensitizer drugs applied in photodynamic therapy is considered a critical issue. This study we introduced new delivery system based on cerium- and silver-doped nanobioactive glass (nBG)/chitosan/collagen composite scaffolds as a carrier for a photosensitizer, methylene blue (MB), in photodynamic therapy, capitalizing on the unique photocatalytic and antibacterial properties of the doped nBG. Cerium and silver offer additional benefits in terms of antimicrobial, anticancer, and wound healing properties. MB is an effective and inexpensive therapeutic photosensitizer compound that exhibits antimicrobial activity through light activation and has shown great potential for selectively treating infected cells. The prepared scaffolds were characterized by FTIR, TGA, and SEM/EDX. The bioactivity and biodegradation were conducted in SBF. The cell viability was carried out against the WI-38 cell line. Finally, the antibacterial efficiency of scaffolds loaded with MB was evaluated before and after laser irradiation against <em>Staphylococcus aureus, Bacillus subtilis (Gram-Positive),</em> and <em>Pseudomonas aerginousea, klebsiella pneumoniae (Gram-negative)</em>. Inhibition of bacteria by reactive oxygen species (ROS) was also measured. Furthermore the primarily MB release profile and kinetic were studied. The results showed that nBG particles increased the thermal stability of the scaffolds, and enhanced the formation of an apatite bone-like layer on the scaffold surfaces. Furthermore, scaffold degradation was tailored by changing the amount of nBG. Moreover, the water uptake of the scaffold containing undoped nBG particles (G010 and G020 samples) showed higher water uptake percentages (682 % and 762 %, respectively) than doped nBG (for 155 % and 322 % for GA10 and GA20, respectively). All scaffolds demonstrated good cell viability at concentrations up to 62.5 μg/ml. However, at higher concentrations, scaffolds incorporating high percentages of nBG were more viable than those containing low amounts of nBG, regardless of the glass composition. The antibacterial tests presented that the MB enhanced scaffold antibacterial efficiency by improving the oxidative stress on bacterial cells, which significantly increased after irradiation. Finally, the release mechanism of MB was diffusion mechanism for scaffolds contained nBG particles, while it did not follow this mechanism for blank polymer scaffolds. In conclusion, these results suggest that the developed multifunctional scaffolds hold significant promise for effective photodynamic antimicrobial chemotherapy in the treatment of microbial infections.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"268 ","pages":"Article 113179"},"PeriodicalIF":3.9,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemiluminescent and fluorescent properties of extracellular red pigment from Talaromyces purpureogenus","authors":"Sharad Bhatnagar ,&nbsp;Wai Lam Tse ,&nbsp;Haruhiko Yoneta ,&nbsp;Yoichi Nakao ,&nbsp;Hideki Aoyagi","doi":"10.1016/j.jphotobiol.2025.113178","DOIUrl":"10.1016/j.jphotobiol.2025.113178","url":null,"abstract":"<div><div><em>Talaromyces purpureogenus</em> is an attractive option for natural pigment production owing to its ability to produce large amounts of water-soluble extracellular red pigments. Recently, natural pigments have been used to develop halochromic biosensing applications, in which the colour change acts as a signal in response to a stimulus; however, the shelf-life and signal generation ability of packaging employing natural pigments maybe suboptimal because of potential reactions with environmental factors. Therefore, this study aimed to develop an alternative method for producing measurable signals from pigments. For this, the chemiluminescent and fluorescent properties of the pigments were explored. The pigment exhibited luminescence when exposed to hydrogen peroxide, which was affected by the reactant concentration, pH levels, and incubation time. Moreover, a rapid signal enhancement exceeding eight-fold was developed by adding luminol. The fluorescence spectrum was strongly affected by pH, with alkaline pH markedly increasing the fluorescence. Fluorescence was also found to be dependent on incubation time, with the maximum signal obtained at pH 12 and 72 h (∼16-fold increase over 0 h), and a model for the increase in fluorescence with time was developed (0.90 &lt; <em>R</em>² &lt; 0.99). The identification of pigment components confirmed the presence of atrorosin R, which had an isochromene/isoquinoline ring, and elucidated the planar structure of this pigment. These findings may aid in developing novel biosensors utilising fungal azaphilone pigments.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"268 ","pages":"Article 113178"},"PeriodicalIF":3.9,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143948476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}