Journal of photochemistry and photobiology. B, Biology最新文献

{"title":"Enhancement of photodynamic reaction by chloroquine through apoptosis induction, lipid peroxidation augmentation, and autophagy inhibition using IR-783","authors":"Natalia Lange ,&nbsp;Wojciech Szlasa ,&nbsp;Dominika Kunachowicz ,&nbsp;Jarosław Kalinin ,&nbsp;Julita Kulbacka","doi":"10.1016/j.jphotobiol.2025.113242","DOIUrl":"10.1016/j.jphotobiol.2025.113242","url":null,"abstract":"<div><div>Photodynamic therapy (PDT) is emerging as a promising approach to enhance cancer treatment by improving therapeutic efficacy, reducing side effects, limiting drug resistance, increasing tumor-specific drug uptake, and preventing metastasis. Second-generation photosensitizers such as IR-783, a cyanine dye, offer high tissue permeability and superior imaging capabilities. This study aimed to evaluate the impact of chloroquine (CQ), an autophagy inhibitor, on the effectiveness of PDT using IR-783 and its potential role in counteracting PDT resistance. Confocal microscopy imaging of the C32 cell line treated with IR-783 revealed a significant increase in caspase-3/−7 activity and enhanced lipid peroxidation following irradiation. CQ exhibited synergistic cytotoxicity when combined with IR-783, reducing cell viability by nearly 50 percentage points. CQ treatment led to increased autophagosome formation and accumulation due to lysosomal impairment, enhancing the sensitivity of cells to IR-783 and highlighting its potential in overcoming PDT resistance.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"271 ","pages":"Article 113242"},"PeriodicalIF":3.7,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144904310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of doxycycline-loaded mesoporous polydopamine nanoparticles for pelvic infection nursing care via synergistic photodynamic therapy","authors":"Shuhua Fan ,&nbsp;Jing Zhang ,&nbsp;Yongna He ,&nbsp;Yi Wang ,&nbsp;Yanru Yu","doi":"10.1016/j.jphotobiol.2025.113243","DOIUrl":"10.1016/j.jphotobiol.2025.113243","url":null,"abstract":"<div><div>Pelvic infection has emerged as a significant health risk to women, making the accurate identification of possible pathogenic bacteria and pathogens crucial for its prevention and treatment. Photodynamic therapy (PDT) for antibacterial purposes has emerged as an alternative and promising therapeutic approach for pelvic infections. In this investigation, we developed new antibacterial nanomaterials (DCMP NPs) using doxycycline (DCL), chitosan (CH), and mesoporous polydopamine (MPD) nanoparticles. Further, we evaluated the physicochemical properties of the DCMP NPs. TEM images indicated that fabricated DCMP NPs were spherical, with a particle size of 160.54 ± 4.56 nm. The findings showed that DCMP NPs (150 μg/mL) demonstrated favorable cytocompatibility in NIH3T3 cells and hemocompatibility, exhibiting remarkable antibacterial efficacy against Gram-negative bacteria (<em>E. coli</em>) and Gram-positive bacteria (<em>S. aureus</em>) following blue laser irradiation (1 W/cm², 450 nm). The antibacterial biofilm impact of DCMP NPs was assessed using Live/Dead staining. The laser-irradiated DCMP NPs demonstrated the most pronounced anti-biofilm impact. Overall, DCMP NPs effectively addressed nursing care for bacterial pelvic infections.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113243"},"PeriodicalIF":3.7,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144996982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Defining safe operational conditions for two-photon imaging with rhodamine B and SYTO 9 dual-staining","authors":"Yong Seok Lee ,&nbsp;Hyun Seok Ryu ,&nbsp;Celine Abueva ,&nbsp;Andrew Padalhin ,&nbsp;So Young Park ,&nbsp;Phil-Sang Chung ,&nbsp;Seung Hoon Woo","doi":"10.1016/j.jphotobiol.2025.113230","DOIUrl":"10.1016/j.jphotobiol.2025.113230","url":null,"abstract":"<div><div>Two-photon microscopy (TPM) frequently employs dual-staining techniques for simultaneous visualization of cellular structure and viability. Despite widespread use, the safety of combining Rhodamine B (RB) and SYTO 9 dyes under 920 nm excitation has not been thoroughly assessed. This study systematically evaluated cytotoxicity, sensitization, and dermal irritation of RB (20 μg/mL) and SYTO 9 (2.7 μg/mL) under typical TPM conditions. BJ fibroblasts and THP-1 monocytic cells were exposed to dye-laser treatments (6–60 mW/cm² for 10 min), followed by CCK-8 viability assays and sensitization evaluation via modified human Cell Line Activation Test (h-CLAT). For dermal irritation assessment, BALB/c mice underwent topical application of dyes and laser exposure, followed by histological and macroscopic evaluations. Results showed maintenance of cell viability (&gt;70 %) across all laser intensities, with no indication of sensitization, as evidenced by low CD54 and CD86 expression, and negligible skin irritation (Primary Irritation Index ≤0.5). Histological analysis confirmed no significant inflammatory or structural damage. These comprehensive findings establish an ISO-compliant safety threshold, confirming that RB and SYTO 9 dual-staining under 920 nm TPM conditions pose no significant biological risks.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"271 ","pages":"Article 113230"},"PeriodicalIF":3.7,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144779285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental study on photodynamic therapy of CCA assisted by electrolytic oxygen supply based on black phosphorus nanocomposites","authors":"Xinqiang Wu ,&nbsp;Xiaofeng Jiang ,&nbsp;Zhaoyuan Liu ,&nbsp;Yuanfeng Gong ,&nbsp;Ping Xue ,&nbsp;Yunqiang Tang","doi":"10.1016/j.jphotobiol.2025.113228","DOIUrl":"10.1016/j.jphotobiol.2025.113228","url":null,"abstract":"<div><div>Cholangiocarcinoma (CCA) is the second most common malignant liver tumor after hepatocellular carcinoma. It is highly aggressive, and most patients are diagnosed at an advanced stage, losing the opportunity for surgical resection. What's worse, the palliative radiotherapy and chemotherapy have limited efficacy. Photodynamic therapy (PDT), based on photosensitizers, is a new treatment modality following surgery and chemoradiotherapy for tumors. Clinically, hematoporphyrin derivative preparations are commonly chosen as photosensitizers for PDT in CCA. Although these derivatives are widely used and their efficacy has been validated by numerous clinical studies, the actual long-term outcome mostly remains unsatisfactory. This is primarily due to their low efficiency in generating reactive oxygen species (ROS) and the inherently hypoxic microenvironment of CCA, which limits their function. To address these concerns, we constructed a nanocomposite based on black phosphorus nanosheets loaded with Fe₃O₄ nanoparticles, assisted by electrolytic oxygen supply. The study aims to enhance the cytotoxicity against CCA by leveraging the efficient singlet oxygen generation of black phosphorus and the Fe₃O₄-catalyzed hydroxyl radical production from hydrogen peroxide. Additionally, it proposes to improve the hypoxic microenvironment of CCA and increase the tumor's sensitivity to PDT by using direct current electrolysis (DCE) to generate oxygen. The primary focus of this research is to explore the regulatory mechanisms of hypoxia-PDT for CCA based on ultrathin black phosphorus nanosheets, providing new insights for optimizing clinical PDT for CCA.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"271 ","pages":"Article 113228"},"PeriodicalIF":3.7,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144768728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-functional fluorescence probe for revealing metabolism during cellular cuproptosis","authors":"Junyan Ma ,&nbsp;Mingtao Zhao ,&nbsp;Dan Meng ,&nbsp;Zilin Jiao ,&nbsp;Weijie Si ,&nbsp;Zhenxing Zhang","doi":"10.1016/j.jphotobiol.2025.113226","DOIUrl":"10.1016/j.jphotobiol.2025.113226","url":null,"abstract":"<div><div>Cuproptosis, a newly identified type of cell death induced by copper, is associated with intracellular oxidative stress, mitochondrial dysfunction and protein aggregation. This work presents the development of a red-emitting fluorescent probe, <strong>COS-NS</strong> which is applied to reveal the possible metabolism during cellular cuproptosis. This probe facilitates the concurrent and accurate detection of HOCl and viscosity through distinct emission wavelengths, thereby eliminating possible interference. Furthermore, probe <strong>COS-NS</strong> has exhibited characteristics like good selectivity, ultrafast response time (∼ 15 s), low detection limit (81 nM), and exceptional stability, rendering it a suitable instrument for detecting cellular HOCl in biological systems. Moreover, we have indicated that cuproptosis could rise oxidative stress and GSH is a key factor in promoting the cuproptosis with sufficient copper ion (II) within biosystem.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"270 ","pages":"Article 113226"},"PeriodicalIF":3.9,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144680576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A photoinduced nuclear translocation of thiophene derivative for selective chemotherapy","authors":"Dat Thanh Dinh ,&nbsp;Yi-Liang Liou ,&nbsp;Tzong-Shiun Li ,&nbsp;Cheng-Chung Chang","doi":"10.1016/j.jphotobiol.2025.113223","DOIUrl":"10.1016/j.jphotobiol.2025.113223","url":null,"abstract":"<div><div>In this study, a novel thiophene-based small organic molecule, 2,5-bis(2-(N-butylpyridinium-4-yl)vinyl)-thiophene diiodine (BBT), was prepared. BBT not only presented selective dark/photo dual cell toxicity to cancer cells but also acted as a nuclear transport inducer for the chemotherapy agent doxorubicin (DXR), thereby promoting anticancer efficacy. BBT exhibits the special aggregation-induced emission (AIE) effect and specifically accumulates in the mitochondria of cancer cells, leading to higher dark toxicity in cancer cells than in normal cells. Interestingly, BBT molecules translocate into the nuclei of cancer cells upon photosensitisation and further selectively exert their phototoxicity on cancer cells. Thanks to the unique characteristics of BBT, a synergistic therapy was optimised based on several combinational treatment pathways between BBT and DXR. The effectiveness of the combinational treatment pathway was evaluated based on the theoretical and actual cell viability rates, the accumulation of DXR in cell nuclei, and the required time interval for the fragmentation of nucleoli. BBT pre-incubation, followed by photosensitisation and then DXR incubation, proved to be the most effective combinational treatment pathway, constituting the synergistic therapy. As a result, BBT is considered a nuclear translocation molecule that can be beneficial for nucleus-targetable chemotherapy drugs to promote nuclear aggregation and drug efficacy. Furthermore, because the nuclear translocation behaviour of BBT is selective in cancer cells, the nuclear aggregation enhancement of chemotherapy drugs can be achieved selectively. In conclusion, we developed a novel synergistic therapy for cancer treatment, and BBT is a potential chemotherapy auxiliary for DXR.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"270 ","pages":"Article 113223"},"PeriodicalIF":3.9,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144662414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural-synthetic hybrid nanoparticles: Polydopamine-Phycocyanin composites for enhanced antibacterial phototherapy against bacterial pathogens","authors":"Armin Fakouri,&nbsp;Matin Mahmoudifard,&nbsp;Nahid Ghorbani,&nbsp;Melika Shafiei,&nbsp;Kambiz Akbari Noghabi","doi":"10.1016/j.jphotobiol.2025.113222","DOIUrl":"10.1016/j.jphotobiol.2025.113222","url":null,"abstract":"<div><div>The rapid rise of antibiotic-resistant bacterial infections demands innovative, non-antibiotic strategies to combat pathogens without driving further resistance. In this study, we developed polydopamine-coated phycocyanin nanoparticles (PDA@PC) by encapsulating the natural antioxidant phycocyanin (PC) within a polydopamine (PDA) matrix. These nanoparticles (146 nm) combine PDA's photothermal and photodynamic properties with PC's antioxidant activity, enabling dual-mode antibacterial action under near-infrared (NIR) laser irradiation. PDA@PC demonstrated excellent biocompatibility (&gt;70 % cell viability) and blood compatibility (&lt;5 % hemolysis), outperforming PDA alone at high doses. Under laser exposure, PDA@PC generated heat and reactive oxygen species (ROS), achieving &gt;50 % inhibition of bacterial biofilms and significant reductions in colony counts for <em>Staphylococcus aureus</em> and <em>Pseudomonas aeruginosa</em>. The nanoparticles also showed enhanced antibacterial efficacy against <em>S. aureus</em>, with minimal inhibitory concentrations (MIC) comparable to conventional antibiotics. By doubling the catechol content of PDA, PDA@PC amplified ROS production while mitigating oxidative stress, offering a safe and effective platform for treating resistant infections. This work highlights the potential of PDA@PC as a multifunctional, laser-activated nano therapy to replace traditional antibiotics and combat the global antimicrobial resistance crisis.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"270 ","pages":"Article 113222"},"PeriodicalIF":3.9,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144633623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of near-infrared polychromatic light in hyperglycemic human cell models: Toward improved diabetic wound healing","authors":"Simge Çıtak Demirez ,&nbsp;Tülay Selin Erkut ,&nbsp;Anıl Sera Çakmak ,&nbsp;Özer Aylin Gürpınar ,&nbsp;Menemşe Gümüşderelioğlu","doi":"10.1016/j.jphotobiol.2025.113224","DOIUrl":"10.1016/j.jphotobiol.2025.113224","url":null,"abstract":"<div><div>Diabetic ulcers are a serious complication of diabetes, often leading to reduced quality of life and, in many cases, limb amputation. These ulcers are characterized by impaired wound healing, primarily due to hyperglycemia-induced inflammation that disrupts normal cellular function. Given the limited effectiveness of conventional therapies, there is a critical need for alternative treatment strategies. This study investigated the therapeutic potential of photobiomodulation (PBM) using polychromatic light in the near-infrared range (600–1200 nm) on human cell types associated with diabetic wound healing, thereby extending previous research predominantly centered on monochromatic light sources. In vitro experiments were conducted using dermal fibroblasts, keratinocytes, and endothelial cells cultured under high-glucose conditions (25–150 mM) to simulate the diabetic environment. A glucose concentration of 150 mM was identified as optimal for modeling hyperglycemia, and PBM treatment was applied accordingly. The protocol consisted of irradiation at an intensity of 2.4 J/cm² per minute, delivered from a distance of 20 cm for 3 min on alternate days over a 7-day culture period. Under these hyperglycemic conditions, PBM significantly enhanced the proliferation and migration of dermal fibroblasts and keratinocytes, restoring these functions to levels comparable to normoglycemic controls. Furthermore, PBM increased fibronectin and collagen synthesis in both control and hyperglycemic groups. In endothelial cells, PBM improved tube formation, which was otherwise impaired under elevated glucose levels. Overall, these results indicate that PBM using a polychromatic light source may serve as a promising adjunctive therapy for enhancing wound healing in diabetic ulcers.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"270 ","pages":"Article 113224"},"PeriodicalIF":3.9,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144653039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A hydroxyl coumarin-chalcone-based fluorescent probe for sensing copper ions in plant and living cells","authors":"Keyi Li ,&nbsp;Yan Huang ,&nbsp;Yatong Sun ,&nbsp;Yiping Zhang ,&nbsp;Yan Zhang ,&nbsp;Baosheng Ren ,&nbsp;Duxia Cao","doi":"10.1016/j.jphotobiol.2025.113218","DOIUrl":"10.1016/j.jphotobiol.2025.113218","url":null,"abstract":"<div><div>Copper ions are essential trace elements for living organisms. However, its excessive levels in the liver and kidneys may lead to severe health issues. Therefore, developing effective fluorescent probes for detecting copper ions is crucial. A hydroxyl coumarin chalcone derivative featuring a hydroxyl substituent has been developed as a fluorescent probe for monitoring Cu²⁺. The probe can selectively detect Cu²⁺ in aqueous solutions, exhibiting a visible color change from red to light yellow to the naked eye. This is accompanied by a quenching of the red fluorescence, with a limit of detection of 11.2 μM. By utilizing Fourier transform infrared spectroscopy, fluorescence spectral changes upon alternating addition of Cu²⁺ and ethylene diamine tetraacetic acid, high-resolution mass spectrometry, and Job's plot analysis, along with a comparison of properties to a structurally analogous compound lacking a hydroxyl substituent, we propose that the probe engages in a 1:1 coordination reaction between its hydroxyl, adjacent carbonyl groups and Cu²⁺. The probe was successfully employed for the quantitative determination of Cu²⁺ content in a variety of real water, beverage and plant samples. Furthermore, the probe exhibits excellent biocompatibility, emitting bright fluorescence under confocal microscopy, which allows for the monitoring of intracellular Cu²⁺ level fluctuations.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"270 ","pages":"Article 113218"},"PeriodicalIF":3.9,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144588701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Femtosecond laser-induced myocardial incision: Preclinical study towards new tools for precision cardiac surgery","authors":"Ang Liu ,&nbsp;Tong Xia ,&nbsp;Siyuan Cao ,&nbsp;He Zhao ,&nbsp;Yubin Hou ,&nbsp;Xuejing Duan ,&nbsp;Li Li ,&nbsp;Ke Wang ,&nbsp;Pu Wang ,&nbsp;Chaowu Yan","doi":"10.1016/j.jphotobiol.2025.113221","DOIUrl":"10.1016/j.jphotobiol.2025.113221","url":null,"abstract":"<div><h3>Background</h3><div>Advanced precise laser techniques for myocardial tissue removal still need to be further explored. Femtosecond laser technology, renowned for its high precision and relatively minor collateral damage in biological tissue processing, shows significant promise for myocardial excision.</div></div><div><h3>Objective</h3><div>This study examined the effects of laser power and scanning speed on myocardial cutting outcomes, as well as to evaluate the feasibility and clinical application potential of femtosecond lasers in myocardial incision.</div></div><div><h3>Methods</h3><div>In this in vitro study using porcine hearts, cubic volumes (1.0 cm³) were laser-cut at varying powers (1.0 W to 5.0 W) and scanning speeds (1.0 mm/s to 4.0 mm/s). A 1064 nm femtosecond fiber laser emitting 179 fs duration pulses at a repetition rate of 500 kHz was used. Digital optical microscopy measured incision width, depth and thermal injury area. Statistical methods including correlation analysis and linear regression were applied to analyze the relationships between variables, and pathological examinations assessed tissue structure and injury extent.</div></div><div><h3>Results</h3><div>Laser power and scanning speed significantly influenced the cutting width, depth, and extent of thermal damage, with laser power played a decisive role andshowed a clear positive correlation with cutting width, depth, and thermal injury. Scanning speed primarily affected cutting efficiency, indirectly influenced cutting outcomes. Pathological examination revealed that increased laser power led to changes in thermal damage to myocardial tissue, characterized by enhanced eosinophilia of myocardial cell cytoplasm.</div></div><div><h3>Conclusions</h3><div>Femtosecond laser technology facilitates precise myocardial incision through meticulous modulation of power and scanning speed, validated exceptional safety and feasibility while highlighting substantial clinical potential. Further research is required to investigate related mechanisms.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"270 ","pages":"Article 113221"},"PeriodicalIF":3.9,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144633624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}