Journal of photochemistry and photobiology. B, Biology最新文献

{"title":"Comparative investigation of structural properties and biological applications of chemical and biogenic synthesis of zirconium dioxide (ZrO2) nanoparticles using Passiflora edulis","authors":"Preetha S,&nbsp;Anilkumar P,&nbsp;Nisha Jenifar A","doi":"10.1016/j.jphotobiol.2024.113089","DOIUrl":"10.1016/j.jphotobiol.2024.113089","url":null,"abstract":"<div><div>Over the last decade, the environmental and wellness cost of antibiotic drug resistance to the societies have been astounding and require urgent attention Metal oxide nanomaterials have been achieved a pull-on deal with its entire applications in biological and photocatalytic applications. The present study conducts a comparative investigation on chemical and biogenic synthesis of zirconium dioxide (ZrO₂) nanoparticles aimed at enhancing their efficacy in their applications. The plant extract of <em>Passiflora edulis</em> act as a reducing and capping properties offering a sustainable and eco-friendly alternative. ZrO₂ nanoparticles have drawn a lot of scrutiny owing to their potential uses in numerous fields, including medicine and environmental remediation. Thereby produced ZrO₂ nanoparticles were synthesized by employing sustainable techniques, and their successful production and their uses were confirmed by characterization by XRD, FTIR, UV–visible spectroscopy, SEM, EDAX, PL, TEM, XPS, TGA and Raman spectroscopy. The zirconia nanoparticles synthesized using chemical and green methods exhibited ultraviolet-visible (UV–Vis) absorption maxima at 221 and 224 nm, respectively, demonstrating their synthesis. X-ray diffraction research revealed that the nanoparticles possess a tetragonal shape, with mean particle sizes of 11 nm and 7 nm, respectively. The synthesized ZrO₂ nanoparticles (ZrO₂ and Ext-ZrO₂) exhibited inhibitory effects against Gram-positive strains (<em>Bacillus subtilis</em> and <em>Staphylococcus aureus</em>) and Gram-negative germs (<em>Escherichia coli</em> and Pseudomonas aureus), with zones of inhibition measuring (12, 8 mm), (8, 11 mm), (12, 15 mm), and (7, 12 mm) correspondingly. The antitumor activity of ZrO₂ and Ext-ZrO₂ was assessed using human colon cancer cells (HT29). The MTT assay was employed to assess the cytotoxicity of ZrO₂ nanospheres on the HT-29 cell line at various concentrations (7.5, 15.6, 31.2, 62.5, 125, 250, and 500 μg/ml). The HT29 cell line exhibits a reduction in cell viability from 96 % to 34 % when the concentration of ZrO₂ nanoparticles escalates. The photocatalytic activity of ZrO₂ and Ext-ZrO₂ exhibited absorbance deterioration at around 445 nm, resulting in the discoloration of Rh B dye under UV light irradiation after 100 min, achieving maximal degradation rates of 96 % and 99 %, respectively. Consequently, the synthesized ZrO₂ and Ext-ZrO₂ may be utilized in antibiotic formulation, pharmaceutical sectors, and photocatalysts.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"263 ","pages":"Article 113089"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142927398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aggregated gold nanoparticles as photoactivators for the photopolymerization of proteins","authors":"Bryce Urian,&nbsp;Rachael Harsch,&nbsp;Lukas Yurasits,&nbsp;Carolyn Proger,&nbsp;Swarna Basu","doi":"10.1016/j.jphotobiol.2025.113099","DOIUrl":"10.1016/j.jphotobiol.2025.113099","url":null,"abstract":"<div><div>Photopolymerization of bovine serum albumin was carried out using reactive oxygen species (ROS) generated by the irradiation of citrate-stabilized gold nanoparticles by a pulsed Nd³⁺:YAG laser. The ROS in this case, singlet oxygen (¹O₂), targets aromatic amino acids within the protein to induce photopolymerization or crosslinking. Other ROS, like the hydroxyl radical, can also form in solution and under high-energy irradiation. The gold nanoparticles were aggregated using different cations in order to maximize singlet oxygen production. Experimental parameters like exposure time and laser power were optimized to minimize damage and maximize crosslinking efficiency, and damage-free crosslinking was observed at laser exposures up to 60 s with samples containing calcium demonstrating most efficient crosslinking. To confirm the role of ROS in crosslinking, the reactive oxygen scavengers sodium azide and mannitol were added at different concentrations to scavenge the singlet oxygen and hydroxyl radical, respectively, and both were observed to stop or slow the formation of crosslinking. The use of gold nanoparticles offers an inert and biocompatible alternative to organic crosslinking agents like rose Bengal and methylene blue.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"263 ","pages":"Article 113099"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzyme-assisted Rosa davurica mitigates UV-induced skin photodamage by modulating apoptosis through Nrf2/ARE and MAPK/NF-κB pathways","authors":"Qiwen Zheng ,&nbsp;Su-Jin Yang ,&nbsp;Eun-Ji Yi ,&nbsp;Se-Jig Park ,&nbsp;Xiangji Jin ,&nbsp;Trang Thi Minh Nguyen ,&nbsp;Gyeong-Seon Yi ,&nbsp;Yong-Jun Jeon ,&nbsp;Tae-Hoo Yi","doi":"10.1016/j.jphotobiol.2025.113098","DOIUrl":"10.1016/j.jphotobiol.2025.113098","url":null,"abstract":"<div><div>Exposure to UV irradiation results in abnormal, extensive apoptosis of skin cells. This excessive cell death can promote inflammation and alter the microenvironment, increasing the risk of skin cancer. Despite extensive research, few materials are effective at simultaneously protecting against both UVA and UVB irradiation. This study aims to develop dual-action material using enzyme-assisted extraction of <em>Rosa davurica</em> Pall (RD) to prevent skin photodamage caused by UVA and UVB irradiation. Three different enzymes were used to assist the extraction of RD, followed by an analysis of the changes in active component levels. Skin photodamage models were established by exposing Normal Human Dermal Fibroblasts (NHDF) and HaCaT cells to UVA and UVB irradiation. The impact of enzyme-assisted extracted RD (ERD) on Reactive Oxygen Species (ROS) production and cell apoptosis was assessed using Flow Cytometry. The effects of ERDs on inflammatory cytokines were measured using ELISA, and RT-PCR was used to evaluate its impact on apoptotic gene expression in photodamaged cells. Furthermore, the impact of ERDs on the Nuclear factor erythroid 2-related factor 2 (Nrf2)/Antioxidant response element (ARE) and Mitogen-activated protein kinases (MAPK)/Nuclear factor-κB (NF-κB) signaling pathways was assessed through Western blot analysis. Finally, the impact of ERDs on full-thickness artificial skin tissue after UV irradiation was assessed using hematoxylin and eosin (H&amp;E) staining. Furthermore, leveraging the experimental results, network pharmacology was utilized to explore the potential of ERDs in preventing skin cancer. Enzyme-assisted extraction enhanced the bioactive components of RD. ERDs effectively reduced ROS levels and suppressed the secretion of Tumor necrosis factor (TNF)-α, Interleukin (IL)-1β, and IL-6 by modulating the Nrf2/ARE and inhibiting the MAPK/NF-κB signaling pathways. This mechanism promoted the expression of the anti-apoptotic gene Bcl-2 and decreased the activity of proapoptotic genes BAX, caspase-3, and caspase-9, thereby countering UV-induced apoptosis. Additionally, staining results demonstrated that ERDs effectively repaired UV-induced photodamage and maintained the integrity of skin structure. ERDs provides comprehensive protection against photodamage induced by UVA and UVB irradiation, demonstrating its potential as an effective photoprotective material and possibly in preventing skin cancer.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"263 ","pages":"Article 113098"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro anti-biofilm efficacy of therapeutic low dose 265 nm UVC","authors":"Sanjay Marasini ,&nbsp;Simon J. Dean ,&nbsp;Simon Swift ,&nbsp;Jagir R. Hussan ,&nbsp;Jennifer P. Craig","doi":"10.1016/j.jphotobiol.2024.113091","DOIUrl":"10.1016/j.jphotobiol.2024.113091","url":null,"abstract":"<div><h3>Purpose</h3><div>Preclinical studies have confirmed the safety and efficacy of narrowband low-intensity ultraviolet C light (UVC) in managing bacterial corneal infection. To further consolidate these findings, the present study aimed to explore <em>in vitro</em> anti-biofilm efficacy of low-intensity UVC light for its potential use in biofilm-related infections.</div></div><div><h3>Methods</h3><div><em>Pseudomonas aeruginosa</em> biofilm was grown in chamber well slides for 48 h and exposed to one of the following challenges: UVC (265 nm wavelength, intensity 1.93 mW/cm²) for 15 s, 30 s, 60 s or 120 s duration, 70% propanol (positive control), or no exposure (negative control). Bacterial LIVE/DEAD staining was conducted at 1 h, 4 h, 6 h and 8 h after challenge exposures to assess the temporal pattern of biofilm inactivation, and slides were imaged using confocal microscopy. Treatment efficacy was quantified by dead biofilm biomass (volume/area - μm³/μm²) for different treatment groups at each time point.</div></div><div><h3>Results</h3><div>At each time point post-exposure, dead biofilm biomass was consistently higher in the alcohol- and UVC-challenged groups than in the unchallenged control (<em>p</em> &lt; 0.05), suggesting a sustained biocidal impact after a given challenge. The quantity of dead biofilm biomass did not differ between UVC groups at any time point (<em>p</em> &gt; 0.05). Observed by confocal microscopy, UVC-exposed biofilm demonstrated predominantly intermediate-stage biofilm (<em>i.e.</em>, dying state) at 1 h, which progressed to dead biofilm by 4 h.</div></div><div><h3>Conclusion</h3><div>Low doses of UVC demonstrated potent anti-biofilm activity, even in exposures as short as 15 s, the dose that has previously been deemed to be effective in managing corneal infection <em>in vivo</em>. These data support the potential for this UVC light-based technology to serve as an affordable, convenient, and effective means of treating ocular infections associated with bacterial biofilm.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"263 ","pages":"Article 113091"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photodynamic antimicrobial therapy with Erythrosin B, Eosin Y, and Rose Bengal for the inhibition of fungal keratitis isolates: An in vitro study","authors":"Brandon Chou ,&nbsp;Katherine Krishna ,&nbsp;Heather Durkee ,&nbsp;Felipe Echeverri Tribin ,&nbsp;Anam Ahmed ,&nbsp;James Lai ,&nbsp;Mariela C. Aguilar ,&nbsp;Braulio C.L.B. Ferreira ,&nbsp;Roger M. Leblanc ,&nbsp;Harry W. Flynn Jr ,&nbsp;Guillermo Amescua ,&nbsp;Jean-Marie Parel ,&nbsp;Darlene Miller","doi":"10.1016/j.jphotobiol.2024.113090","DOIUrl":"10.1016/j.jphotobiol.2024.113090","url":null,"abstract":"<div><h3>Introduction</h3><div>Fungal keratitis is a leading cause of corneal blindness, with current antifungal treatments having limited efficacy. One promising treatment modality is Rose Bengal (RB) photodynamic antimicrobial therapy (PDAT) that has shown mixed success against fungal keratitis. Therefore, there is a need to explore the antimicrobial efficacy of other green-light activated photosensitizers that have deep penetration in the cornea to combat the deep fungal infections, such as Erythrosin B (EB) and Eosin Y (EY).</div></div><div><h3>Objective</h3><div>This study will explore PDAT inhibitory effects with different photosensitizers, RB, EB, and EY against two common fungal ocular isolates, <em>Aspergillus</em> spp. and <em>Fusarium</em> spp.</div></div><div><h3>Methods</h3><div>Twelve fungal isolates (<em>Fusarium</em> spp., <em>n</em> = 6, <em>Aspergillus</em> spp., n = 6) were prepared in suspension for evaluation of growth inhibition to PDAT with three photosensitizers, EB, EY, and RB. Custom green light source (λ = 518 nm, energy density = 5.4 J/cm²) was applied to the experimental groups for 15 min. Fungal growth inhibition was assessed after experimentation by analyzing the area of growth within the irradiated zone on agar plates.</div></div><div><h3>Results</h3><div>All twelve fungal isolates showed no inhibition to EB, EY, and RB without irradiation. <em>Fusarium</em> spp. were more susceptible to PDAT than <em>Aspergillus</em> spp. In all <em>Fusarium solani</em> strains, all photosensitizers with light showed full inhibition within the 47 mm diameter irradiation zone.</div></div><div><h3>Conclusion</h3><div>EB, EY, and RB PDAT demonstrated comparable antifungal inhibition against six <em>Fusarium</em> ocular isolates; these findings in conjunction with the deeper tissue penetration of EB and EY, are of interest to treat more advanced and deeper cases of fungal keratitis.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"263 ","pages":"Article 113090"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142927399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"α-arbutin prevents UVA-induced skin photodamage via alleviating DNA damage and collagen degradation in NIH-3T3 cells","authors":"Nan Zhao,&nbsp;Xin Nie,&nbsp;Yizhen Yan,&nbsp;Zhao Liu,&nbsp;Xueqing Chen,&nbsp;Peng Shu,&nbsp;Jiangming Zhong","doi":"10.1016/j.jphotobiol.2025.113100","DOIUrl":"10.1016/j.jphotobiol.2025.113100","url":null,"abstract":"<div><div>Ultraviolet radiation (UV) causes certain side effects to the skin, and their accumulation to a certain extent can lead to accelerated aging of the skin. Recent studies suggest that α-arbutin may be useful in various disorders such as hyperpigmentation disorders, wound healing, and antioxidant activity. However, the role of α-arbutin in skin photodamage is unclear. In this study, under UVA-induced photodamage conditions, α-arbutin treated mouse skin fibroblasts (NIH-3T3) can repair DNA damage and resist apoptosis by reducing the production of reactive oxygen species (ROS) and increasing the phosphorylation of glycogen synthase kinase 3 beta (GSK3β) to orchestra AKT/GSK3β pathway. Meanwhile, α-arbutin can also regulate collagen metabolism and facilitate the replenishment of collagen by targeting the phosphorylation of SMAD3 to mediate the TGFβ/SMAD pathway in NIH-3T3. In conclusion, we found that α-arbutin can mitigate the detrimental effects of skin photodamage induced by UVA irradiation, and provides a theoretical basis for the use of α-arbutin in the treatment of skin photodamage.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"263 ","pages":"Article 113100"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single amino acid substitution analogs of marine antioxidant peptides with membrane permeability exert a marked protective effect against ultraviolet-B induced damage","authors":"Yichao Huang ,&nbsp;Qian He ,&nbsp;Peipei Zhang ,&nbsp;Juxingsi Song ,&nbsp;Yangkai Wang ,&nbsp;Shaoqian Zhu ,&nbsp;Yongfei Lv ,&nbsp;Dayuan Zhou ,&nbsp;Yanan Hu ,&nbsp;Liming Zhang ,&nbsp;Guoyan Liu ,&nbsp;Qianqian Wang","doi":"10.1016/j.jphotobiol.2025.113120","DOIUrl":"10.1016/j.jphotobiol.2025.113120","url":null,"abstract":"<div><div>Ultraviolet-B (UVB) causes oxidative stress, which is implicated in skin damage and photoaging. Antioxidant peptides exhibit protective effects against UVB-induced oxidative stress and are thus regarded as potential competitors compared to synthetic antioxidants for cosmetics. In the present study, we provided a discovery pipeline for screening and modifying marine-derived antioxidant peptides, and successfully identified and characterized three novel modified peptides (WP5, LW5 and YY6) with strong antioxidant abilities. Their scavenging activities on 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate) radical (ABTS·) and hydroxyl radical (·OH) were higher than those of glutathione (GSH) (ABTS·: 71.12 ± 3.58 %, 67.63 ± 1.65 % and 68.51 ± 0.54 % by WP5, LW5 and YY6, respectively, vs 61.51 ± 1.02 % by GSH; ·OH: 52.15 ± 1.99 %, 51.25 ± 1.29 % and 53.06 ± 2.23 % by WP5, LW5 and YY6, respectively, vs 42.69 ± 1.18 % by GSH). The modified peptides can effectively penetrate cell membrane and significantly enhance cell viability against UVB-induced oxidative stress in human keratinocyte (HaCaT) cells by reducing the levels of reactive oxygen species and malondialdehyde and increasing the activity of intracellular antioxidant enzymes, including superoxide dismutase and glutathione peroxidase. Additionally, the modified peptides decreased the expression of tumor necrosis factor-α, interleukin-6 and interleukin-1β in UVB-induced cell inflammatory response, exhibiting a potent anti-inflammatory activity. Further investigation into the molecular mechanism revealed that the modified peptides not only decreased cell apoptosis by down-regulating the apoptosis factors Bax/Bcl-2 and c-PARP, but also increased the antioxidant capacity of HaCaT cells by interrupting the interaction between Kelch-like ECH associated protein 1 (Keap1) and nuclear factor erythroid 2-related factor 2 (Nrf2), and ultimately promoting Nrf2 activation. The findings suggest a promising strategy for accelerating the discovery of antioxidant peptides and cell-penetrating peptides, providing valuable insights for pharmaceutical and cosmetic industries.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"264 ","pages":"Article 113120"},"PeriodicalIF":3.9,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143241303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ICG-ALA complex for improved phototherapy of cancer","authors":"Mahshid Hashemkhani ,&nbsp;Alphan Sennaroğlu ,&nbsp;Havva Yağci Acar","doi":"10.1016/j.jphotobiol.2025.113121","DOIUrl":"10.1016/j.jphotobiol.2025.113121","url":null,"abstract":"<div><div>5-Aminolevulinic acid (ALA) based photodynamic therapy (PDT) is a clinically approved therapeutic method for cancer treatment. Indocyanine green (ICG) is on the other hand an FDA-approved fluorescent dye that has been widely used in medical imaging in the near-infrared (NIR), and lately recognized as an agent to induce photothermal therapy (PTT). However, the hydrophilicity of ALA and rapid degradation of ICG in aqueous or physiological media as well as their instability limit their clinical application. Besides, the combination of PDT and PTT is a promising alternative to a single therapy approach. Herein, electrostatic binding of ALA to ICG is proposed to bypass such handicaps and provide enhanced therapeutic outcomes with simultaneous PDT and PTT combination. ICG-ALA exhibited excellent biocompatibility up to 50 μg ICG/mL-10 mM ALA in the dark in both SKBR3 and MDA-MB-231 cell lines with higher cell uptake compared to free ALA or ICG. ICG-ALA treatment coupled with 640/808 nm 5 min co-irradiation caused significantly stronger phototoxicity in both cancer cell lines at very low concentrations, reaching near complete loss of viability at 2.5 μg ICG /mL-0.5 mM ALA equivalent concentration of the ICG-ALA. The temperature increase observed during irradiation of the cells and the elevated oxidative stress resulting in the release of caspase 3/7 agrees well with the onset of PTT and PDT. In addition, ICG-ALA demonstrates visualization of cancer cells in both NIR (ICG) and visible (PpIX) regions allowing imaging-guided phototherapy.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"264 ","pages":"Article 113121"},"PeriodicalIF":3.9,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143350542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pulsed red light photobiomodulation ameliorates oxytocin-induced primary dysmenorrhea in mice by inhibiting oxidative stress and lipid accumulation","authors":"Qiqi Fu ,&nbsp;Jiali Yang ,&nbsp;Hui Jiang ,&nbsp;Haokuan Qin ,&nbsp;Angze Li ,&nbsp;Longfei Huo ,&nbsp;Muqing Liu","doi":"10.1016/j.jphotobiol.2025.113119","DOIUrl":"10.1016/j.jphotobiol.2025.113119","url":null,"abstract":"<div><div>Photobiomodulation (PBM) has gained attention as a kind of anti-pain or anti-inflammation therapy, yet its efficacy in mitigating the symptoms and underlying metabolic disturbances of primary dysmenorrhea remains underexplored. Here, 630 nm light reduced menstrual pain and prostaglandin F_2a/prostaglandin E₂ dysregulation, regulated oxidation and lipid peroxidation levels, and improved uterus damage in oxytocin-induced mice. Notably, pulsed wave (PW) treatment exhibited superior efficacy compared to continuous wave application. Hence, this research focused on the effects of 630 nm PW on oxytocin-induced mice by examining changes in the uterine transcriptome and plasma metabolome. Results from integrated analyses revealed significant modifications primarily in antioxidant and lipid metabolism pathways, alongside shifts in biomarkers related to arachidonic acid metabolism. Quantitative real-time PCR confirmed the downregulation of critical genes associated with oxidative stress and inflammation, as well as the suppression of uterine smooth muscle contractions and lipid overaccumulation. These findings support the potential of 630 nm PW PBM as a viable option for clinical interventions in dysmenorrhea management.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"264 ","pages":"Article 113119"},"PeriodicalIF":3.9,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungal derived dye as potential photosensitizer for antimicrobial photodynamic therapy","authors":"Isabelle Almeida de Lima ,&nbsp;Caio de Azevedo Lima ,&nbsp;Sarah Raquel de Annunzio ,&nbsp;Fernanda de Oliveira ,&nbsp;Silvio Silvério da Silva ,&nbsp;Carla Raquel Fontana ,&nbsp;Valéria de Carvalho Santos-Ebinuma","doi":"10.1016/j.jphotobiol.2025.113116","DOIUrl":"10.1016/j.jphotobiol.2025.113116","url":null,"abstract":"<div><div>Photodynamic therapy (PDT) combines light with a photosensitizing agent to target and destroy abnormal cells or pathogens, offering a non-invasive and precise approach. Applying microbial dyes in PDT presents a great opportunity because these compounds may absorb specific wavelengths of light, generating reactive oxygen species (ROS) that induce oxidative stress, leading to cell or microbial death. This study evaluated the extract of <em>Talaromyces amestolkiae</em> containing azaphilone red dyes obtained from cultivation process as photosensitizer (PS) in antimicrobial photodynamic therapy (aPDT). Initially the crude extract was obtained in incubator shaker varying the culture media composition. Following, the crude extract containing the red dyes exhibited non-toxicity in dark conditions across all concentrations tested. PDT experiments with different amounts of the crude extract at a light dose of 80 J.cm^-2 and upon irradiation at 460 nm was studied. A complete reduction of <em>Escherichia coli</em> and approximately 2 log₁₀ reductions of <em>Staphylococcus aureus, Cutibacterium acnes</em> and <em>Enterococcus faecalis</em> was achieved using 25 % (v.v^-1) of the crude extract while 50 % (v.v^-1) of the crude extract led to a complete reduction of both <em>E. coli</em> and <em>S. aureus</em>, and around 5 log₁₀ reductions of <em>C. acnes</em> and <em>E. faecalis</em>. Importantly, minimal photodegradation of the PS occurred during irradiation across all concentrations studied. These findings highlight the potential of <em>T. amestolkiae</em>-derived red dyes extract for use in aPDT, demonstrating non-toxicity in the absence of light, good aqueous solubility, high photostability, and strong microbial reduction capabilities under specific light conditions.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"264 ","pages":"Article 113116"},"PeriodicalIF":3.9,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143350541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}