Journal of photochemistry and photobiology. B, Biology最新文献

{"title":"Acute UV response of early erythema and late edema in SKH1 mice","authors":"Szabolcs Bozsányi ,&nbsp;Rhea Carmel Glen Rodrigues ,&nbsp;Ruby Acquah ,&nbsp;Erin C. Tracy ,&nbsp;Sean P. Murphy ,&nbsp;Jocelyn Tracy ,&nbsp;Li Yan ,&nbsp;Mehdi Boostani ,&nbsp;Hamid-Reza Rezvani ,&nbsp;Wendy J. Huss ,&nbsp;Gyorgy Paragh","doi":"10.1016/j.jphotobiol.2026.113369","DOIUrl":"10.1016/j.jphotobiol.2026.113369","url":null,"abstract":"<div><div>In human skin, erythema assessed 24 h after ultraviolet (UV) exposure is a non-invasive method for determining the acute UV response. Although erythema is often reported in murine models as a measure of acute UV response, the most informative timepoint after UV exposure is unclear because of variability in mouse strain, sex, and light source. Our current work addresses this critical gap by elucidating the development of erythema and edema over time after acute UV irradiation with solar-simulator UV (SSUV) or UVB light in female or male SKH1 mice. Cutaneous erythema was assessed with ImageJ on digital photographs, and edema was assessed using optically guided high-frequency ultrasound (OG-HFUS) to measure skin thickness and a visual edema scale up to 72 h after UV exposure. Erythema was observed between 4 and 10 h in both female and male mice at most doses of SSUV or UVB irradiation. Erythema induced by irradiation doses of 200, 300, 400 mJ/cm² peaked earlier at the 4–6 h timepoint with SSUV irradiation compared to the same doses of UVB that peaked at the 6–8 h timepoint. At 24 h, edema-induced blanching of the skin masked the erythema, resulting in the highest-dose area having a lower erythema index than non-irradiated areas. Edema was dose-dependent (with SSUV exposure) and peaked between 24 and 48 h using both the OG-HFUS and visual edema scale. Erythema measurement at 4–8 h after UV is optimal for assessing the acute UV response in the SKH1 mouse model at lower irradiation doses. Whereas edema measured at 24–48 h after UV exposure is a sex-neutral and reliable universal measure of acute UV response in SKH1 mouse skin at high UV irradiation doses.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"275 ","pages":"Article 113369"},"PeriodicalIF":3.7,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146010861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photoperiod alters the diurnal rhythm pattern of testicular transcription and metabolism in Brandt's voles","authors":"Hong Sun ,&nbsp;Wenbo Xu ,&nbsp;Maolin Huang ,&nbsp;Jieyu Huang ,&nbsp;Wenju Yang ,&nbsp;Qianqian Zhang ,&nbsp;Lijuan Zhao ,&nbsp;Dawei Wang ,&nbsp;Zhenlong Wang","doi":"10.1016/j.jphotobiol.2026.113367","DOIUrl":"10.1016/j.jphotobiol.2026.113367","url":null,"abstract":"<div><div>Photoperiod changes serve as critical environmental signals for seasonally reproducing animals, with their decoding dependent on the precise functioning of endogenous circadian clocks. Here we conducted a comprehensive analysis of diurnal transcripts and metabolites profiles in the testes of Brandt's voles (<em>Lasiopodomys brandtii</em>) under long photoperiod (LP) and short photoperiod (SP), to elucidate the role of circadian rhythms within peripheral testicular tissue in regulating reproduction. Phenotypic results demonstrated that LP promoted testicular development, whereas SP suppressed it. Then we observed relatively weaker transcriptional and metabolic rhythmicity in the testes under both photoperiods. The function of diurnal rhythmic genes was mainly related to homeostasis, developmental, reproductive behavior, motility and sperm flagellum assembly pathways, suggesting that diurnal clocks act as photoperiodic timekeeper for reproductive timing. In contrast, testosterone biosynthesis and key energy metabolism pathways essential for spermatogenesis were largely governed by non-rhythmic genes expression patterns. Together, these findings underscore the non-uniform pervasiveness of diurnal rhythmicity in testis, where reproductive initiation driven by the diurnal clock, while subsequent spermatogenic processes progressively deviate from 24 h rhythmicity.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"275 ","pages":"Article 113367"},"PeriodicalIF":3.7,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145979924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Saccharomyces/pearl ferment lysate filtrate repairs UVB-induced skin barrier damage by regulating Nrf2/HO-1 and JNK/MAPK signaling pathways","authors":"Tanglin Liu ,&nbsp;Biao Guo ,&nbsp;Liying Tong ,&nbsp;Hao Li ,&nbsp;Caibing Wang ,&nbsp;Kai Zang ,&nbsp;Ruili Zhao ,&nbsp;Xinyi Zhao ,&nbsp;Chenglong Ye ,&nbsp;Xiyun Ye ,&nbsp;Yongyan Dang","doi":"10.1016/j.jphotobiol.2026.113366","DOIUrl":"10.1016/j.jphotobiol.2026.113366","url":null,"abstract":"<div><div>Pearls have skin whitening and antioxidant properties, but their effects on skin barrier are less understood. This study investigated the reparative effects of saccharomyces/pearl ferment lysate filtrate (PFL) on UVB-induced skin barrier damage. In HaCaT cells, PFL restored proteins related to epidermal differentiation, tight junctions, and moisture retention, all reduced by UVB exposure, and lowered the expression of inflammatory factors. Similarly, in a UVB-induced skin damage mouse model, PFL significantly alleviated skin peeling, erythema, TWEL and epidermal thickening, while also suppressing UVB-induced skin inflammation. Mechanistically, PFL promoted Nrf2 nuclear translocation and upregulated antioxidant proteins NQO1 and HO-1, hereby decreasing ROS accumulation. It also inhibited the activation of the c-Jun N-terminal kinase (JNK) pathway in response to UVB-induced oxidative stress, likely due to the activation of Nrf2. These findings indicate that PFL may repair UVB-induced skin barrier damage through activation of the Nrf2/HO-1 pathway and inhibition of the JNK/MAPK pathway, offering potential as a therapeutic agent for skin barrier repair.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"275 ","pages":"Article 113366"},"PeriodicalIF":3.7,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145979921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancement of laser-induced vasodilation by medicinal plants: The eNOS-dependent and antioxidant actions of Panax ginseng and Angelica keiskei","authors":"Luis Henrique Oliveira de Moraes ,&nbsp;Camila Pereira Sabadini ,&nbsp;Nayara Formenton da Silva ,&nbsp;Thiago Augusto do Nascimento ,&nbsp;Tereza Cristina Buzinari ,&nbsp;Natália Fernanda do Couto ,&nbsp;Shane A. Phillips ,&nbsp;Gerson Jhonatan Rodrigues","doi":"10.1016/j.jphotobiol.2025.113351","DOIUrl":"10.1016/j.jphotobiol.2025.113351","url":null,"abstract":"<div><div>Photobiomodulation (PBM) is a promising non-pharmacological approach to improve vascular function via nitric oxide (NO)-mediated pathways. However, its effectiveness can be limited under conditions of endothelial dysfunction. This study investigated whether <em>Panax ginseng</em> and <em>Angelica keiskei</em>, two medicinal plants known for their antioxidant and vasorelaxant properties, can enhance PBM-induced vasodilation through mechanisms involving endothelial nitric oxide synthase (eNOS). Aortic rings from Wistar rats were treated with increasing doses of a standardized plant extract combination and exposed to 660 nm laser irradiation. Optimal doses (50–200 mg/kg) significantly potentiated PBM-induced vasodilation, an effect abolished by pharmacological inhibition of NO signaling and endothelium removal. In a chronic eNOS inhibition model (L-NAME), the plant combination did not restore PBM effects but partially recovered acetylcholine-induced vasorelaxation, suggesting endothelial compensation. In mesenteric arteries, the herbal treatment improved acetylcholine sensitivity but did not alter flow-induced or laser-induced vasodilation, especially under hypertensive conditions. These findings highlight a synergistic interaction between phytotherapy and PBM, mediated primarily by eNOS activation and redox modulation, with potential translational relevance for vascular disorders.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"275 ","pages":"Article 113351"},"PeriodicalIF":3.7,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145979923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disinfection mechanisms of gram-negative and gram-positive bacteria through multi-target damage under UV-C light at 222 and 254 nm","authors":"Keisuke Naito ,&nbsp;Akihiro Shirai","doi":"10.1016/j.jphotobiol.2026.113362","DOIUrl":"10.1016/j.jphotobiol.2026.113362","url":null,"abstract":"<div><div>Ultraviolet-C (UV-C) irradiation is a promising approach for inactivating viruses and microorganisms. Germicidal mercury lamps emitting at 254 nm and krypton chloride (KrCl) excimer lamps emitting at 222 nm both possess sterilization properties, but the KrCl lamp is considered less harmful to humans. UV-C at 222 and 254 nm induces cyclobutene pyrimidine dimers (CPDs) in <em>Escherichia coli</em> (<em>E. coli</em>). Although <em>E. coli</em> irradiated at 254 nm can undergo photoreactivation, cells exposed to 222 nm cannot, as CPDs are not effectively repaired. This study aimed to investigate the molecular mechanisms underlying bacterial inactivation by UV-C irradiation at 222 and 254 nm. We measured the absorbance of key bacterial components–peptidoglycan (PG), membrane proteins, phospholipids, and DNA–in Gram-negative and Gram-positive bacteria and confirmed the photodecomposition. At 222 nm, a substantial fraction of the light was absorbed by the membrane proteins, and the cleavage of peptide bonds resulted in structural damage and leakage of cellular contents, leading to cell death. In Gram-positive bacteria, which contain thick PG layers, part of the UV-C was absorbed by PG. Irradiation with 222-nm light of PG and proteins resulted in peptide bond cleavage, liberating amino acids. Degradation of the bacterial cell envelope increased the permeability and efflux of intracellular substances, leading to membrane rupture and, ultimately, bacterial cell death. These findings demonstrate that 222-nm irradiation exerts bactericidal effects through distinct mechanisms in Gram-negative and Gram-positive bacteria.</div></div><div><h3>Synopsis</h3><div>The Gram-negative <em>E. coli</em> strain NBRC 106373 and the Gram-positive <em>S. aureus</em> strain NBRC 12732 undergo DNA mutations when irradiated at 222 nm. Simultaneously, they are killed by the leakage of cellular content. This is because the strong light absorption of peptidoglycan and membrane proteins damages the cell structure.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"275 ","pages":"Article 113362"},"PeriodicalIF":3.7,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145952494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bi-functional activity of chlorophyllin: Antifungal action against Botrytis cinerea and induction of grapevine defense genes","authors":"Koffi Vincent Messanh,&nbsp;Mohammad Muhie Ddine,&nbsp;Veronica Ambrosini,&nbsp;Catherine Riou","doi":"10.1016/j.jphotobiol.2026.113365","DOIUrl":"10.1016/j.jphotobiol.2026.113365","url":null,"abstract":"<div><div><em>Botrytis cinerea (B. cinerea)</em> is a fungus with a high mutation rate and infects more than 200 plant species, causing significant yield losses. Therefore, new strategies to fight against this ubiquitous phytopathogen are highly sought after. In this context, antimicrobial Photodynamic Treatment (aPDT) using the natural chlorophyllin named E140 as a photosensitizer is considered to be a good and efficient approach to limit <em>B. cinerea</em> growth and its spore germination and viability while limiting its mutational power. In this study, we showed that E140 tested at 100 μM under a 16 h photoperiod significantly slowed down <em>B. cinerea</em> mycelium growth without affecting spore germination. Moreover, as E140 was localized in the hyphal cell wall structure, this could explain the reduced septal length and width under a 16 h photoperiod, leading to a global reduction in mycelial growth. Unexpectedly, E140 was shown to reduce the expression of two virulence genes (<em>BcBac</em> and <em>BcBcg</em>3) and, on detached grapevine leaves, to increase the expression of general defense genes such as <em>PR1</em>, <em>PR3,</em> and <em>PR4.</em> Stilbene synthase (<em>STS</em>) and heat shock hypersensitive response (<em>HSR1)</em>. Furthermore, as we also showed in this study, E140 did not alter the development of grapevine plantlets and had no toxic effect on housefly maggots. Thus, water-soluble standalone E140 could be considered as a fungistatic molecule that is also able to alter Botrytis virulence and induce plant protection, suggesting a great new potential of E140 for further applications in viticulture and agriculture.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"275 ","pages":"Article 113365"},"PeriodicalIF":3.7,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145929215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Time-resolved fluorescence imaging of methylene blue reveals heterogeneous polarity in living cells","authors":"Andrey V. Belashov ,&nbsp;Anna A. Zhikhoreva ,&nbsp;Zhenlong Huang ,&nbsp;Fangrui Lin ,&nbsp;Irina V. Semenova ,&nbsp;Oleg S. Vasyutinskii ,&nbsp;Junle Qu","doi":"10.1016/j.jphotobiol.2026.113368","DOIUrl":"10.1016/j.jphotobiol.2026.113368","url":null,"abstract":"<div><div>The paper presents time-resolved fluorescence analysis of methylene blue (MB) in solutions and in living cells in vitro. The analysis of MB fluorescence lifetime in solutions of different pH, viscosity and polarity revealed its independence on acidity and viscosity and linear rise with decreasing polarity. Moreover, MB binding to albumin and DNA did not affect its fluorescence lifetime. The obtained dependence of MB fluorescence lifetime on the Lippert-Mataga polarity parameter enabled analysis of polarity distributions in living cells. Fluorescence-lifetime images of MB fluorescence in cancerous HeLa and pseudo-normal bEnd.3 cells provided clear double-exponential signals, which were suggested to be due to diversity of polarity in different cell compartments. The longer fluorescence lifetime and its contribution were shown to differ in cells of different lines, that allowed us to suggest that polarity of low-polar structures and their amount differ in cells of these lines. In cells of both lines the fluorescence lifetimes in nuclei were shorter than those in cytoplasm. The combined analysis of fluorescence lifetimes and phasor plot coordinates allowed for segmentation of the intracellular area to regions of different polarity corresponding to nuclei and cytoplasm with the accuracy of about 90%, and to reveal differences in cells of the two lines.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"275 ","pages":"Article 113368"},"PeriodicalIF":3.7,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145979333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultraviolet radiation reshapes the transcriptomic landscape of human skin aging: Insights from a multi-age comparative study","authors":"Jiaqi Zhang ,&nbsp;Xueli Jia ,&nbsp;Qitian Fu ,&nbsp;Xiaofeng Bai ,&nbsp;Jun Wang ,&nbsp;Yi Qin ,&nbsp;Jie Yang ,&nbsp;Fengwei Qi ,&nbsp;Yao Pan","doi":"10.1016/j.jphotobiol.2025.113352","DOIUrl":"10.1016/j.jphotobiol.2025.113352","url":null,"abstract":"<div><h3>Background</h3><div>Skin aging arises from both intrinsic processes and extrinsic factors, with ultraviolet (UV) radiation being the primary extrinsic cause of photoaging. However, the molecular mechanisms that differentiate these processes across the human lifespan remain incompletely characterized.</div></div><div><h3>Objective</h3><div>This study aimed to comprehensively compare the dynamic transcriptomic profiles of photoaged (neck, high UV exposure) and intrinsically aged (chest, low UV exposure) skin across three age groups (young, middle-aged, elderly), and to integrate these findings with biophysical skin measurements.</div></div><div><h3>Methods</h3><div>We performed transcriptomic analysis on skin biopsies from the neck and chest of 30 healthy female volunteers (<em>n</em> = 10 per age group). This was followed by differential gene expression, Gene Ontology (GO), and KEGG pathway enrichment analyses. The molecular findings were then correlated with an extensive panel of biophysical skin parameters assessing barrier function, elasticity, pigmentation, and microstructure.</div></div><div><h3>Results</h3><div>Photoaged neck skin exhibited accelerated age-dependent transcriptomic dysregulation, marked by enrichment in pathways related to DNA damage response (e.g., CHEK1), stress signaling (e.g., MAPK/STK3), metabolic reprogramming (e.g., AMPK/PPARG), and oncogenic transformation (e.g., WNT10B). A persistent pseudo-inflammatory state, mirrored by herpes simplex virus 1 infection pathway enrichment, was also observed. Notably, sirtuin expression (SIRT1, SIRT5) was severely depleted in photoaged skin, with SIRT1 specifically linked to attenuated AMPK signaling in middle age. In contrast, intrinsic aging in chest skin involved a more gradual decline in homeostatic processes like metabolism and immune vigilance. Comparative analysis further revealed UV-specific disruption in gap junction assembly and cytoskeletal organization, and in elderly skin, activation of pathways associated with neurodegenerative diseases. Finally, canonical correlation analysis (CCA) confirmed strong links between key gene expression patterns (e.g., FGFBP1 with erythema, CHEK1 with age) and clinical skin aging phenotypes.</div></div><div><h3>Conclusion</h3><div>Our study provides a high-resolution molecular map of human skin aging, demonstrating that UV radiation does not merely accelerate but fundamentally rewires the aging network, driving pathways distinct from intrinsic aging. Key identified drivers include sirtuin depletion, aberrant stress signaling, and a chronic pseudo-inflammatory response, offering novel targets for anti-photoaging interventions.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"275 ","pages":"Article 113352"},"PeriodicalIF":3.7,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145897903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultraviolet-treated riboflavin induces ROS-mediated apoptosis via inhibiting mitochondrial complex I in acute myeloid leukemia","authors":"Ruining Liu ,&nbsp;Shuang Ge ,&nbsp;Jinyuan Sun ,&nbsp;Yi Liu ,&nbsp;Liping Sun ,&nbsp;Yang Yu ,&nbsp;Deqing Wang","doi":"10.1016/j.jphotobiol.2025.113347","DOIUrl":"10.1016/j.jphotobiol.2025.113347","url":null,"abstract":"<div><div>Acute myeloid leukemia (AML) remains challenging due to drug resistance and relapse, and novel therapeutic approaches are urgently needed. Here, we demonstrated that ultraviolet-treated riboflavin (RF-UV) elicited strong antileukemia effects in AML cell lines and primary patient samples, while showing minimal toxicity in normal cells. Mechanistically, inhibition of mitochondrial respiratory Complex I by RF-UV elevated reactive oxygen species (ROS) levels and resulted in ROS-mediated apoptosis, endoplasmic reticulum (ER) stress and mitochondrial dysfunction. Crucially, in vivo research showed RF-UV considerably slowed the development of AML and extended the survival time of mice. Our research unveiled the clinical application potential of RF-UV as a complex I inhibitor in leukemia treatment.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"274 ","pages":"Article 113347"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145827953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin-mediated photodynamic action disturbs TOM70-depedent MIC60 import to damage mitonchondria against breast cancer","authors":"Xiang-Yi Zhao ,&nbsp;Min Yan ,&nbsp;Liang Zheng ,&nbsp;Chang-Long Gou ,&nbsp;Qi Huang ,&nbsp;Liu-Gen Li ,&nbsp;Xin-Ran Yu ,&nbsp;Jing-Yu Lu ,&nbsp;Cui Hu ,&nbsp;Si-Han Zhang ,&nbsp;Cunqing Kong ,&nbsp;Fan Leng ,&nbsp;Tong-Fei Li","doi":"10.1016/j.jphotobiol.2025.113339","DOIUrl":"10.1016/j.jphotobiol.2025.113339","url":null,"abstract":"<div><div>The regulation of mitochondrial membrane proteins is of crucial significance for breast cancer therapy. TOM70, which located in mitochondria outer membrane, could import MIC family molecules to preserve mitochondrial homeostasis. However, there are few agents targeting TOM70. Therein, the effects of curcumin and it's mediated photodynamic therapy (PDT) on the TOM70 and mitochondrial function for breast cancer treatment were investigated. The 4 T1 and MDA-MB-231 cells were utilized as the breast cancer cells. The 4 T1 cell-bearing mice were constructed as the breast cancer animal model. The anti-cancer efficacy was validated using the CCK-8, Annexin-V/PI staining, colony formation. The associated molecules were detected by Western blots (WB), RT-qPCR, and Immunohistochemistry (IHC). The target was verified by molecular docking, CETSA, and DARTS. The mitochondrial proteins and DNA were extracted for the MIC60 and mtDNA damage detection. Curcumin treatment showed poor efficacy in the breast cancer model, as characterized by cell viability, apoptosis, proliferation of breast cancer cells, and the growth of tumor grafts in mice. However, curcumin-mediated PDT inhibited breast cancer in vitro and in vivo. Further exploration identified curcumin bond to TOM70, which is highly expressed in breast cancer, thereby activating it. But curcumin-induced PDT inactivated TOM70 through generated reactive oxygen species (ROS), which in turn interfered with the binding of MIC60 and its translocation into mitochondria. Curcumin-triggered PDT led to severe mitochondrial damage compared with the curcumin treatment, which could be blocked by the N-Acetylcysteine (NAC). Additional TOM70 rescue dampened curcumin PDT-mediated mitochondrial damage and anti-breast cancer efficacy. To summarize, the present research identifies curcumin-induced PDT inactivated TOM70, thereby attenuating MIC60 import, leading to mitochondrial damage against breast cancer. We propose a novel approach to tumor treatment through the regulation of mitochondrial membrane proteins using the phytomedicine-driven PDT.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"274 ","pages":"Article 113339"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145794102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}