Rapid penetrating inactivation and disinfection through ice layers by light-condensed deep ultraviolet LED irradiation

IF 3.9 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology Pub Date : 2025-04-14 DOI:10.1016/j.jphotobiol.2025.113154

Lucheng Yu , Peng Shen , Zefeng Lin , Shini Yang , Maowei Yang , Yaxiao Lou , Shiqiang Lu , Feiya Xu , Shengrong Huang , Xueqiong Sun , Jiamin He , Huachun Wang , Yanhua Zhang , Canming Zhang , Fawen Su , Fuchun Xu , Xiaohong Chen , Ling Li , Duanjun Cai , Junyong Kang

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引用次数: 0

Abstract

The longer survival of virus and bacteria under low temperature greatly accelerates the infection risk and duration. How to effectively and rapidly inactivate them beneath ice layer in cold chain logistics has been an important issue never being well studied. Here, we demonstrate a novel light condenser design of high power UVC-LED device for rapid penetrating inactivation through ice layers. With fish-scale, ellipsoidal and alumina coated condenser, a portable UVC (275 nm) LED device in ultrahigh light output power (> 3 W) was fabricated in a narrowed propagation angle (< 60^o). Such condensed light beams are able to overcome the interfacial refringence at the ice and bubble interfaces in different types of ice layers for enhancing the transmission rate. As a result, the penetrating UVC light could give rise to the effective necrosis and apoptosis influence as well as DNA/RNA destruction on cells. A 99.99 % inactivation rate of Escherichia coli and Staphylococcus aureus has been obtained through 5-mm-thickness opaque ice layer. By 1.5 s irradiation, SARS-COV-2 virus can be completely inactivated and disinfected. This work proved the strong power of UVC-LED for cold chain disinfection applications.

Abstract Image

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采用光凝深紫外LED照射，通过冰层快速穿透灭活消毒

病毒和细菌在低温下存活时间的延长，大大加快了感染的风险和持续时间。在冷链物流中，如何在冰层下有效、快速地灭活它们一直是一个重要的问题，但从未得到很好的研究。在这里，我们展示了一种新型的高功率UVC-LED器件的光聚光器设计，用于快速穿透冰层灭活。采用鱼鳞、椭球体和氧化铝涂层聚光镜，一种超高光输出功率的便携式UVC (275 nm) LED器件(>；3w)在窄传播角(<；60 o)。这种压缩光束能够克服不同类型冰层中冰和泡界面处的界面折射，从而提高传输速率。因此，穿透性UVC光可以对细胞产生有效的坏死和凋亡影响以及DNA/RNA破坏。通过5mm厚度的不透明冰层，大肠杆菌和金黄色葡萄球菌的灭活率达到99.99%。辐照1.5 s可使SARS-COV-2病毒完全灭活和消毒。这项工作证明了UVC-LED在冷链消毒应用中的强大功能。

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来源期刊

Journal of photochemistry and photobiology. B, Biology 生物-生化与分子生物学

CiteScore

12.10

自引率

1.90%

发文量

161

审稿时长

37 days

期刊介绍： The Journal of Photochemistry and Photobiology B: Biology provides a forum for the publication of papers relating to the various aspects of photobiology, as well as a means for communication in this multidisciplinary field. The scope includes: - Bioluminescence - Chronobiology - DNA repair - Environmental photobiology - Nanotechnology in photobiology - Photocarcinogenesis - Photochemistry of biomolecules - Photodynamic therapy - Photomedicine - Photomorphogenesis - Photomovement - Photoreception - Photosensitization - Photosynthesis - Phototechnology - Spectroscopy of biological systems - UV and visible radiation effects and vision.