Journal of photochemistry and photobiology. B, Biology最新文献

{"title":"Quinolinium-substituted porphyrins as promising antimicrobial photosensitizers","authors":"Leticia D. Costa ,&nbsp;Cátia Vieira ,&nbsp;Mariana Q. Mesquita ,&nbsp;Steffen Hackbarth ,&nbsp;M. Graça P.M.S. Neves ,&nbsp;Adelaide Almeida ,&nbsp;Augusto C. Tomé ,&nbsp;M. Amparo F. Faustino","doi":"10.1016/j.jphotobiol.2025.113275","DOIUrl":"10.1016/j.jphotobiol.2025.113275","url":null,"abstract":"<div><div>Characterized by a remarkable chemical versatility and outstanding photophysical properties, porphyrins stand out as one of the most promising classes of photosensitizers for antimicrobial photodynamic therapy (aPDT). In this work, we described the synthesis, as well as the chemical and photophysical characterization of quinolinium-substituted porphyrins, and their corresponding Zn(II) complexes. The efficacy of these porphyrins in the photoinactivation of bacteria was evaluated, both alone and combined with the co-adjuvant potassium iodide, against methicillin-resistant <em>Staphylococcus aureus (</em>MRSA, Gram-positive) and <em>Escherichia coli</em> (Gram-negative) strains.</div><div>Overall, the results demonstrated the high potential of both cationic porphyrins to effectively photoinactivate bacterial strains. Cationic derivative <strong>2</strong>, at a concentration of 1.0 μM, achieved reductions exceeding &gt; 99.99999 % (&gt;7.0 log₁₀ CFU mL⁻¹) for <em>S. aureus</em> after 10 min of white light irradiation (25 mW cm⁻²) and &gt; 99.9 % (3.52 log₁₀ RLU) for <em>E. coli</em> after 60 min aPDT treatment. Similarly, the Zn(II) counterpart <strong>2a</strong> also showed excellent efficiency, achieving ∼99.999 % reduction (4.95 log₁₀ CFU mL⁻¹) against MRSA after 60 min of aPDT at 1.0 μM, and a 3.52 log₁₀ RLU reduction for <em>E. coli</em> after 30 min of PDT at 5.0 μM. The action mechanism of both cationic quinolinium-substituted porphyrins demonstrated to be dependent on the singlet oxygen production, consistent with a Type II photodynamic pathway. The use of potassium iodide (KI) as co-adjuvant led to the production of reactive iodine species (RIS), namely iodine, and increased the photodynamic effect.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113275"},"PeriodicalIF":3.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145251346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adjunctive 810 nm photobiomodulation with pharmacotherapy for trigeminal neuralgia: A randomized controlled trial in a tertiary care centre","authors":"Saumya Shukla,&nbsp;Adit Srivastava,&nbsp;Sakshi Batra,&nbsp;Subhadeep Pal,&nbsp;Sivani Darjee,&nbsp;Amlendu Shekhar","doi":"10.1016/j.jphotobiol.2025.113249","DOIUrl":"10.1016/j.jphotobiol.2025.113249","url":null,"abstract":"<div><h3>Background</h3><div>Trigeminal neuralgia (TN) is a debilitating orofacial pain disorder. Pharmacotherapy with carbamazepine is the mainstay of treatment, but adverse effects and tolerance often limit its long-term use. Low-level laser therapy (LLLT) has shown promise in managing various neuropathic pains, yet no study has assessed its efficacy as an adjunct in TN treatment within an Indian population.</div></div><div><h3>Methods</h3><div>In this single-center randomized controlled trial, 40 patients with classical TN were randomly allocated into two groups: Group I received carbamazepine alone, and Group II received carbamazepine plus LLLT (using an 810 nm diode laser at 200 mW for 30s per point). Pain intensity was assessed using the Numeric Rating Scale (NRS) at baseline, 1, 2, and 3 weeks, and the McGill Pain Questionnaire at baseline and after 3 months. LLLT was administered thrice weekly for 3 weeks at 810 nm, delivering 6 J per irradiation point. Outcomes were evaluated by a blinded assessor.</div></div><div><h3>Results</h3><div>Baseline NRS scores were similar (8.50 ± 0.95 vs. 8.75 ± 0.96, <em>p</em> = 0.412). At 3 weeks, mean NRS reduced significantly more in Group II (0.40 ± 0.68) than Group I (3.45 ± 1.23), <em>p</em> &lt; 0.001. McGill scores at 3 months were significantly lower in Group II (2.90 ± 3.07) compared to Group I (23.40 ± 6.38), p &lt; 0.001. None in Group II required carbamazepine dose escalation during the study, whereas all patients in Group I needed increases.</div></div><div><h3>Conclusion</h3><div>Combined photobiomodulation with carbamazepine provided significantly superior pain relief compared to medication alone in TN patients, reducing the need for higher drug doses. This is the first study to demonstrate the benefits of LLLT as an adjunct therapy in TN among the Indian population, indicating it as a promising option for adjunctive management.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113249"},"PeriodicalIF":3.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144989528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In situ sonoporation to enhance the tumour uptake of silicon phthalocyanine and improve PDT effectiveness in a triple negative breast cancer murine model","authors":"Martina Capozza ,&nbsp;Alberto Mangia ,&nbsp;Michela Gagliardi ,&nbsp;Rachele Stefania ,&nbsp;Francesca Garello ,&nbsp;Laura Conti ,&nbsp;Enzo Terreno","doi":"10.1016/j.jphotobiol.2025.113266","DOIUrl":"10.1016/j.jphotobiol.2025.113266","url":null,"abstract":"<div><div>The effectiveness of photodynamic therapy (PDT) has been well demonstrated <em>in vitro</em>, but <em>in vivo</em> studies have only shown a delay in tumour growth. Tumour recurrence is often reported in clinical trials and is usually associated with limited tumour uptake of the photosensitiser (PS). In this study, sonoporation (SNP) is utilised as a physical targeting tool to enhance the uptake of an untargeted PS in cells and tumour tissues. Using a chemometric approach, we identified the optimal sonoporation stimulus to maximise uptake and cell viability (frequency 1.05 MHz, tON (percentage of positive signal in a single pulse): 50 %, DC: 90 %, burst: 1 s, sonoporation time 1 min). We achieved a viability of 83.9 % ± 10.3, and an uptake in live cells of 53.1 % ± 6.6. The combination of sonoporation and photodynamic therapy resulted in a significant reduction in cell viability. In a triple-negative breast cancer model, sonoporation combined with photodynamic therapy significantly inhibited tumour growth. For the first time, our results highlight the potential of sonoporation as a non-invasive method to increase the intratumour uptake of photosensitisers, offering a promising strategy to improve PDT efficacy.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113266"},"PeriodicalIF":3.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of doxycycline-loaded mesoporous polydopamine nanoparticles for pelvic infection nursing care via synergistic photodynamic therapy","authors":"Shuhua Fan ,&nbsp;Jing Zhang ,&nbsp;Yongna He ,&nbsp;Yi Wang ,&nbsp;Yanru Yu","doi":"10.1016/j.jphotobiol.2025.113243","DOIUrl":"10.1016/j.jphotobiol.2025.113243","url":null,"abstract":"<div><div>Pelvic infection has emerged as a significant health risk to women, making the accurate identification of possible pathogenic bacteria and pathogens crucial for its prevention and treatment. Photodynamic therapy (PDT) for antibacterial purposes has emerged as an alternative and promising therapeutic approach for pelvic infections. In this investigation, we developed new antibacterial nanomaterials (DCMP NPs) using doxycycline (DCL), chitosan (CH), and mesoporous polydopamine (MPD) nanoparticles. Further, we evaluated the physicochemical properties of the DCMP NPs. TEM images indicated that fabricated DCMP NPs were spherical, with a particle size of 160.54 ± 4.56 nm. The findings showed that DCMP NPs (150 μg/mL) demonstrated favorable cytocompatibility in NIH3T3 cells and hemocompatibility, exhibiting remarkable antibacterial efficacy against Gram-negative bacteria (<em>E. coli</em>) and Gram-positive bacteria (<em>S. aureus</em>) following blue laser irradiation (1 W/cm², 450 nm). The antibacterial biofilm impact of DCMP NPs was assessed using Live/Dead staining. The laser-irradiated DCMP NPs demonstrated the most pronounced anti-biofilm impact. Overall, DCMP NPs effectively addressed nursing care for bacterial pelvic infections.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113243"},"PeriodicalIF":3.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144996982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3, 7-dihydroxy-2, 4-dimethoxyphenanthrene protects against UVB-induced skin hyperpigmentation via antioxidant and anti-melanogenic mechanisms","authors":"Mengyan Li ,&nbsp;Die Li ,&nbsp;Yu Zhang ,&nbsp;Juan Wang ,&nbsp;Ziying Zhang ,&nbsp;Weiqun Wang ,&nbsp;Shaochun Chen ,&nbsp;Lechun Lyu ,&nbsp;Weimin Liu","doi":"10.1016/j.jphotobiol.2025.113277","DOIUrl":"10.1016/j.jphotobiol.2025.113277","url":null,"abstract":"<div><h3>Background</h3><div>Excessive reactive oxygen species (ROS) produced by UVB radiation can disrupt the normal redox balance, leading to oxidative cellular damage as well as triggering melanin synthesis by melanocytes. Currently, natural active substances are emphasized in UV protection research. This study explored the protective effect of 3, 7-dihydroxy-2, 4-dimethoxyphenanthrene (DDP), a bioactive compound from <em>Dendrobium</em> lindleyi Stendel, against UVB-induced skin hyperpigmentation and examined its specific mechanism.</div></div><div><h3>Methods</h3><div>To evaluate the anti- melanogenic and antioxidant activities of DDP in vitro, molecular docking was used to predict the binding conformation of DDP to tyrosinase (TYR) and melanocortin 1 receptor (MC1R). Subsequently, CCK8 and cell live/dead staining were used to screen the safe concentration range. B16-F10 cells were treated with different doses of DDP before UVB light irradiation and before α-melanocyte-stimulating hormone (α-MSH) induction, and the evaluation of cells included determination of melanin content, Western blotting analysis, TYR activity assay, ROS assay, DPPH• and ABTS•⁺ radical scavenging assays. A brown guinea pig model was used to further evaluate the protective effect of DDP on UVB-induced skin pigmentation in vivo, and compared with commercial skin care products.</div></div><div><h3>Results</h3><div>In the present study, Molecular docking showed that DDP binds to TYR and MC1R better than Kojic Acid. DDP was found to possess excellent anti-melanogenic and antioxidant activities in vitro. DDP was effective in alleviating skin melanin accumulation induced by UVB irradiation without inflammatory side effects.</div></div><div><h3>Conclusions</h3><div>DDP protects against UVB-induced skin hyperpigmentation via antioxidant and anti-melanogenic mechanisms.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113277"},"PeriodicalIF":3.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145313119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual near-infrared wavelength photobiomodulation accelerates transdermal burn wound repair via anti-inflammatory, pain relief and redox-regulating mechanisms","authors":"Ashok Priyadarshi,&nbsp;Gaurav K. Keshri,&nbsp;Divya Yadav,&nbsp;Asheesh Gupta","doi":"10.1016/j.jphotobiol.2025.113267","DOIUrl":"10.1016/j.jphotobiol.2025.113267","url":null,"abstract":"<div><div>Burns cause severe tissue damage and impose a significant psychological and financial burden, highlighting the need for innovative, effective, and cost-efficient therapeutic interventions for tissue repair. Research indicates that multimodal therapy enhances healing by positively regulating various phases of tissue repair. Photobiomodulation therapy (PBMT), which employs low-power light, offers a promising non-pharmacological energy-based healing approach for non-healing wounds. Absorbed photons stimulate cellular metabolism, increase ATP production, and modulate molecular signaling pathways, thereby facilitating the repair process. In this study, we investigated the effects of combined NIR pulsed 810 nm and superpulsed 904 nm lasers PBMT on inflammation, pain, and redox status during transdermal burn wound healing in rats. Animals were divided into five groups, namely uninjured, control (burn), pulsed 810 nm, superpulsed 904 nm lasers, and dual wavelength PBMT. Combined PBMT exhibited a positive synergistic effect on the burn repair process by significantly (<em>P</em> &lt; 0.05) reducing inflammation (NF-κB, TNF-α, IL-1β, NOS-2, IL-6), pain (substance P-receptor, COX-2), and oxidative stress (ROS, 4-HNE adduct, LPO, 3-NT), while increasing redox potential/endogenous antioxidant (Nrf2, catalase), and maintaining cytoprotection (GRP78) seven days post-wounding. These molecular findings were well-supported by histopathological analysis, which indicated decreased mast cell infiltration, exhibiting the potent anti-inflammatory effects of the combined PBMT. Altogether, the current findings demonstrate that dual PBMT synergistically accelerates the repair of full-thickness burns, exhibits analgesic, anti-inflammatory responses, reduces oxidative stress, activates the antioxidant defense pathway, and maintains cellular redox homeostasis. This non-invasive dual NIR PBMT presents a promising translational therapeutic approach for burn repair in clinical care.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113267"},"PeriodicalIF":3.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145091974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of new chlorin e6 derivatives bearing galactose moieties on the macrocyclic periphery and their structure–activity relationships","authors":"Marina V. Mal'shakova ,&nbsp;Nikolay D. Belykh ,&nbsp;Lydia N. Shestakova ,&nbsp;Natalia E. Galochkina ,&nbsp;Artem O. Belotelov ,&nbsp;Sergey S. Soroko ,&nbsp;Irina V. Balalaeva ,&nbsp;Dmitry V. Belykh ,&nbsp;Natalia Yu. Shilyagina","doi":"10.1016/j.jphotobiol.2025.113268","DOIUrl":"10.1016/j.jphotobiol.2025.113268","url":null,"abstract":"<div><div>Recent studies of photosensitizers have increasingly focused on improving their aqueous solubility, reducing dark toxicity and enhancing photodynamic activity. In our work, we have synthesized and characterized a series of chlorin e₆ derivatives varying in the number of galactose moieties in the molecule and way they are linked to the chlorin macrocycle. We established that the quantity and localization of galactose moieties in the macrocycle affects both dark and photoinduced cytotoxicity. All of the studied derivatives exhibited singlet oxygen quantum yield photogeneration comparable to that of the clinically approved chlorin e₆ dimeglumine (Photoditazine®) with marginal exceeding. Although the incorporation of galactose moieties to the periphery of the chlorin macrocycle led to slightly decreased photostability of resulting derivatives, it did not significantly hinder their photodynamic activity. Moreover, cellular uptake of the obtained derivatives was significantly improved compared to that of Photoditazine®. Collectively, favorable spectral, photochemical, photobiological characteristics, along with improved aqueous solubility, suggest that chlorin e₆ derivatives with peripheral galactose substituents are promising photosensitizers for clinical applications.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113268"},"PeriodicalIF":3.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145251390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative theoretical study on the photoisomerization and antioxidant capacity of resveratrol and its Azo/Dihydro analogs","authors":"Lei Wang ,&nbsp;Lingling Wang ,&nbsp;Chaofan Sun","doi":"10.1016/j.jphotobiol.2025.113251","DOIUrl":"10.1016/j.jphotobiol.2025.113251","url":null,"abstract":"<div><div>This study employs a suite of quantum chemical methods to systematically investigate the photoisomerization mechanism and antioxidant activity of resveratrol (Res) and two key derivatives, Azo-Resveratrol (AzoRes) and Dihydro-Resveratrol (dhRes), thereby elucidating the impact of molecular scaffold modification on their structure-activity relationships. Employing density functional theory (DFT), time-dependent DFT (TD-DFT), spin-flip TD-DFT and multistate complete active space second-order perturbation theory (MS-CASPT2), we investigated the geometric configurations, absorption spectra, photoisomerization pathways, and key antioxidant parameters for all three molecules. The results reveal that the substitution of the C<img>C bond with an N<img>N linkage (AzoRes) induces a bathochromic shift in the absorption spectrum, introduces a low-energy n → π* transition, and facilitates a barrierless photoisomerization pathway. Conversely, the saturation of the C<img>C bond to a C<img>C single bond (dhRes) disrupts the π-conjugated system, resulting in a significant hypsochromic shift. Analysis of frontier molecular orbitals and global descriptive parameters established the order of S₀-state antioxidant capacity as AzoRes &gt; Res &gt; dhRes, a trend that correlates with the narrower HOMO-LUMO gap of AzoRes compared to Res (by 0.302 eV and 0.564 eV for trans and cis isomers, respectively). While photoexcitation universally enhanced the antioxidant potential of all molecules, it also reordered the efficacy to Res &gt; AzoRes &gt; DHRes in the S₁ state. Our findings elucidate the intrinsic relationship between the molecular scaffold, photochemical behavior, and antioxidant activity, providing a theoretical framework for the rational design of novel photo-responsive antioxidant materials.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113251"},"PeriodicalIF":3.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144934096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LED light emission and olive pomace-rich culture medium in potentiating bacterial cellulose synthesis and improving biopolymer properties","authors":"Paula Rodrigues ,&nbsp;Halima Khelifa ,&nbsp;Tatiana B. Schreiner ,&nbsp;Noureddine Halla ,&nbsp;Maria Filomena F. Barreiro ,&nbsp;Pedro J.L. Crugeira","doi":"10.1016/j.jphotobiol.2025.113287","DOIUrl":"10.1016/j.jphotobiol.2025.113287","url":null,"abstract":"<div><div>The growing demand for sustainable and high-performance materials has driven research into optimizing bacterial cellulose (BC) production using innovative strategies. In this context, this work aimed to evaluate the enhancement of BC biosynthesis by <em>Komagataeibacter intermedius</em>, combining LED photobiomodulation with a natural olive pomace-based medium (20 % MOP) and their impact on the properties of the produced BC. Hestrin-Schram medium was used as the control. LED irradiation (630 nm) was applied at 14 J/cm² (control LED; 20 % MOP LED) in static culture for 7 days at 28 °C. The MOP medium and LED emission demonstrated a synergistic effect, resulting in a significant production increase of 385.97 % compared to the control. The BC membranes synthesized in the 20 % MOP and LED irradiation exhibited improved thermal stability, stress capacity (247.06 %), and strain at break (33.96 %) compared to the control. The production-to-quality ratio, the valorization of the by-product, and the use of the energy-efficient emitter are key factors for the scalability and viability of the bioprocess and its industrial application.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113287"},"PeriodicalIF":3.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145362160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasmonic photothermal based quick sterilization of surgical tools and pathogen suspensions","authors":"Sarabjot Kaur ,&nbsp;Parminder Kaur ,&nbsp;Shobit Attery ,&nbsp;Vrushali Raka ,&nbsp;Rajneesh Dadwal ,&nbsp;Hemraj Nandanwar ,&nbsp;Sanjeev Soni","doi":"10.1016/j.jphotobiol.2025.113286","DOIUrl":"10.1016/j.jphotobiol.2025.113286","url":null,"abstract":"<div><div>This study investigates the efficacy of plasmonic photothermal technique for quick sterilization of surgical tools and pathogen suspensions for the elimination of a high load of broad-spectrum pathogens viz. <em>ESKAPE</em> pathogens - <em>Candida albicans</em> (5 × 10⁷ CFU/mL of each pathogen). Here, the contaminated surgical tools and the corresponding pathogens suspension to be sterilized, are irradiated with electromagnetic radiation (Visible-NIR) in the presence of synthesized triangular silver nanoplates leading to localized heat generation through plasmonic photothermal phenomenon. The irradiation source including the sterilization device prototype is developed <em>In-house</em> and is used for the sterilization of reusable surgical tools. The sterilization is achieved at lower temperatures of ∼65–70 °C, at atmospheric pressure within 10 min of total duration. Also, sterility of the nanoplates suspension after a treatment cycle is confirmed for its re-use for multiple subsequent sterilization cycles. The demonstrated sterilization efficacy ∼7.69 Log₁₀ reductions of each pathogen, meets the required sterility assurance level (SAL) norms of 6 Log₁₀ reduction. Further, the mechanism behind sterilization efficacy of plasmonic photothermal technique shows the morphological alterations &amp; membrane lysis along with the combined impact of reactive oxygen species (ROS), which disrupts cellular metabolic pathways within the cells. In-vivo investigation of the use of a surgical blade sterilized through plasmonic photothermal technique, on BALB/C mice, further strengthens the real-time application and safety. Contrary to the antimicrobial studies reported in literature for such phenomenon, as far as we are aware, it is the first reported instance of the sterilization for reusable surgical tools through plasmonic photothermal technique.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113286"},"PeriodicalIF":3.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145362161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}