Journal of photochemistry and photobiology. B, Biology最新文献_第10页

The copepod Acartia spinicauda feeds less and dies more under the influences of solar ultraviolet radiation and elevated pCO2 在太阳紫外线辐射和 pCO2 升高的影响下，桡足类 Acartia spinicauda 摄食更少，死亡更多。

IF 3.9 2区生物学

Journal of photochemistry and photobiology. B, Biology Pub Date : 2024-08-29 DOI: 10.1016/j.jphotobiol.2024.113020

Weijia Chen , Xuyang Wang , Mark L. Wells , Kunshan Gao

{"title":"The copepod Acartia spinicauda feeds less and dies more under the influences of solar ultraviolet radiation and elevated pCO2","authors":"Weijia Chen , Xuyang Wang , Mark L. Wells , Kunshan Gao","doi":"10.1016/j.jphotobiol.2024.113020","DOIUrl":"10.1016/j.jphotobiol.2024.113020","url":null,"abstract":"<div><p>While solar ultraviolet radiation (UVR) is known to impact zooplankton, little has been documented on its impacts under elevated pCO<sub>2</sub>. Here, we show that exposure to UVR decreased the feeding and survival rates of the copepod <em>Acartia spinicauda</em>, that artificial UV-B of 2.25 W·m<sup>−2</sup> for 4 h resulted in a 52 % inhibition of its grazing rates and a 45 % reduction in survival rates compared to visible light alone. On the other hand, an increase in pCO<sub>2</sub> to 1000 μatm (pH drop of 0.4) immediately and significantly increased the UVR-induced inhibition of feeding. Subsequently, the combination of the high pCO<sub>2</sub> (1000 μatm) and UVR resulted in about 65 % lethal impact, with UV-A contributing 21 % and UV-B 44 % compared to the visible light alone and ambient pCO<sub>2</sub> conditions. While the copepod was shown to be able to sense and escape from UV-exposed areas, these findings suggest that UVR impacts on the copepod can be exacerbated with progressive ocean acidification or in high CO<sub>2</sub> waters, including upwelled regions.</p></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"260 ","pages":"Article 113020"},"PeriodicalIF":3.9,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142149864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of near-infrared Photobiomodulation therapy on the ion content of 50B11 sensory neurons measured through XRF analysis 通过 XRF 分析测量近红外光生物调节疗法对 50B11 感觉神经元离子含量的影响

IF 3.9 2区生物学

Journal of photochemistry and photobiology. B, Biology Pub Date : 2024-08-23 DOI: 10.1016/j.jphotobiol.2024.113019

Luisa Zupin , Alessandra Gianoncelli , Fulvio Celsi , Valentina Bonanni , George Kourousias , Pietro Parisse , Murielle Salomé , Sergio Crovella , Egidio Barbi , Giuseppe Ricci , Lorella Pascolo

{"title":"The effect of near-infrared Photobiomodulation therapy on the ion content of 50B11 sensory neurons measured through XRF analysis","authors":"Luisa Zupin , Alessandra Gianoncelli , Fulvio Celsi , Valentina Bonanni , George Kourousias , Pietro Parisse , Murielle Salomé , Sergio Crovella , Egidio Barbi , Giuseppe Ricci , Lorella Pascolo","doi":"10.1016/j.jphotobiol.2024.113019","DOIUrl":"10.1016/j.jphotobiol.2024.113019","url":null,"abstract":"<div><p>Photobiomodulation therapy (PBMT) is a form of treatment commonly used for routine clinical applications, such as wound healing of the skin and reduction of inflammation. Additionally, PBMT has been explored for its potential in pain relief.</p><p>In this work, we investigated the effect of PBMT on ion content within the 50B11 sensory neurons cell line in vitro using X-Ray fluorescence (XRF) and atomic force microscope (AFM) analysis.</p><p>Two irradiation protocols were selected utilizing near-infrared laser lights at 800 and 970 nm, with cell fixation immediately following irradiation.</p><p>Results showed a decrease in Calcium content after irradiation with both protocols, and with lidocaine, used as an analgesic control. Furthermore, a reduction in Potassium content was observed, particularly evident when normalized to cellular volume.</p><p>These findings provide valuable insights into the molecular impact of PBMT within 50B11 sensory neurons under normal conditions. Such understanding may contribute to the wider adoption of PBMT as a therapeutic approach.</p></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"259 ","pages":"Article 113019"},"PeriodicalIF":3.9,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1011134424001799/pdfft?md5=da1fea496ddc549ae3e55bc26d9bfed5&pid=1-s2.0-S1011134424001799-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142098939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the immune-modulating power of THz-FEL irradiation 揭示太赫兹-FEL辐照的免疫调节能力

IF 3.9 2区生物学

Journal of photochemistry and photobiology. B, Biology Pub Date : 2024-08-22 DOI: 10.1016/j.jphotobiol.2024.113017

Qi Zhang , Weijun Wang , Sen Shang , Xu Li , Tingting Zhao , Peng Zhang , Dai Wu , Kui Zhou , Xiaoyun Lu

{"title":"Unveiling the immune-modulating power of THz-FEL irradiation","authors":"Qi Zhang , Weijun Wang , Sen Shang , Xu Li , Tingting Zhao , Peng Zhang , Dai Wu , Kui Zhou , Xiaoyun Lu","doi":"10.1016/j.jphotobiol.2024.113017","DOIUrl":"10.1016/j.jphotobiol.2024.113017","url":null,"abstract":"<div><p>As terahertz (THz) technology advances, the interaction between THz radiation and the living body, particularly its effects on the immune system, has attracted extensive attention but remains poorly understood. This study firstly elucidated that exposure to 3 THz-FEL radiation markedly suppressed contact hypersensitivity reactions in mice induced by DNFB, as evidenced by a reduction in ear thickness and a discernible recovery in the Th1/Th2 cell balance. 3 THz irradiation led to cellular stress in the irradiated skin locale, increasing the levels of IL-4 and IL-10 and modulating the activity and migration of dendritic cells and mast cells. Furthermore, THz irradiation precipitated a rapid alteration in the skin lipidome, altering several categories of bioactive lipids. These findings offer new insights into the immunomodulatory effects of THz radiation on living organisms and the potential underlying mechanisms, with implications for the development of therapeutic approaches in managing skin allergic diseases.</p></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"259 ","pages":"Article 113017"},"PeriodicalIF":3.9,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142122204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural variation in photosynthetic electron transport of wheat flag leaves in response to dark-induced senescence 小麦旗叶的光合电子传递随暗诱导衰老的自然变化

IF 3.9 2区生物学

Journal of photochemistry and photobiology. B, Biology Pub Date : 2024-08-22 DOI: 10.1016/j.jphotobiol.2024.113018

Cheng Yang , Zishan Zhang , Yuan Yuan , Deqi Zhang , Haiyang Jin , Ying Li , Simeng Du , Xiangdong Li , Baoting Fang , Fang Wei , Ge Yan

{"title":"Natural variation in photosynthetic electron transport of wheat flag leaves in response to dark-induced senescence","authors":"Cheng Yang , Zishan Zhang , Yuan Yuan , Deqi Zhang , Haiyang Jin , Ying Li , Simeng Du , Xiangdong Li , Baoting Fang , Fang Wei , Ge Yan","doi":"10.1016/j.jphotobiol.2024.113018","DOIUrl":"10.1016/j.jphotobiol.2024.113018","url":null,"abstract":"<div><p>Early leaf senescence affects photosynthetic efficiency and limits growth during the late production stage of winter wheat (<em>Triticum aestivum</em>). Natural variation in photosystem response to senescence represents a valuable resource for improving the aging traits of flag leaves. To explore the natural variation of different phases of photosynthetic electron transport in modern wheat cultivars during senescence, we exposed the flag leaves of 32 wheat cultivars to dark conditions to induce senescence process, and simultaneously measured prompt fluorescence and modulated 820 nm reflection. The results showed that the chlorophyll content, activity of PSII donor side, PSI and electron transfer between PSII and PSI were all decreased during dark-induced senescence, but they showed different sensitivity to dark-induced senescence. Furthermore, natural variation in photosynthetic parameters among the 32 wheat cultivars were also observed and showed by variation coefficient of the different parameters. We observed that PSII and PSI activity showed less sensitivity to dark-induced senescence than electron transfer between them, while PSII and PSI activity exhibit greater natural variation than electron transport between PSII and PSI. It suggests that Cyt<sub>b6f</sub> might degrade faster and have less variation than PSII and PSI during dark-induced senescence.</p></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"259 ","pages":"Article 113018"},"PeriodicalIF":3.9,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142048700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Betahistine mesylate reduces the damage of blue light exposure in Drosophila model 甲磺酸倍他司汀可减少蓝光照射对果蝇模型的伤害

IF 3.9 2区生物学

Journal of photochemistry and photobiology. B, Biology Pub Date : 2024-08-10 DOI: 10.1016/j.jphotobiol.2024.113009

Xiangyu Li, Zhiwei Zhao, Jianan He, Jie Shen

{"title":"Betahistine mesylate reduces the damage of blue light exposure in Drosophila model","authors":"Xiangyu Li, Zhiwei Zhao, Jianan He, Jie Shen","doi":"10.1016/j.jphotobiol.2024.113009","DOIUrl":"10.1016/j.jphotobiol.2024.113009","url":null,"abstract":"<div><p>Previous studies have demonstrated the efficacy of betahistine mesylate in treating vertigo and angioneurotic headache, enhancing microcirculation, and facilitating histamine release. However, limited research has been conducted on the drug's potential in mitigating blue light-induced damage. Thus, this study utilized <em>Drosophila</em> as the model organism and employed the Siler model to investigate the impact of various concentrations of betahistine mesylate on the lifespan, under 3000 lx blue light irradiation. At the same time we measure food intake, spontaneous activity, and sleep duration of <em>Drosophila</em>. The findings of this study indicate that a high concentration of betahistine mesylate can decrease the initial mortality (b0) in male flies, mitigating the damage of blue light to <em>Drosophila</em>. Consequently, this delays the aging process in male <em>Drosophila</em> and extends their average lifespan. After betahistine mesylate ingestion, locomotor activity upon blue light exposure decreased significantly in male <em>Drosophila</em>. In conclusion, this study offers initial evidence supporting the investigation of the regulatory mechanisms of betahistine mesylate on lifespan and its potential anti-blue light effects.</p></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"259 ","pages":"Article 113009"},"PeriodicalIF":3.9,"publicationDate":"2024-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141978967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Near-infrared pH-switchable BODIPY photosensitizers for dual biotin/cRGD targeted photodynamic therapy 用于生物素/cRGD 双靶向光动力疗法的近红外 pH 可切换 BODIPY 光敏剂

IF 3.9 2区生物学

Journal of photochemistry and photobiology. B, Biology Pub Date : 2024-08-10 DOI: 10.1016/j.jphotobiol.2024.113010

Martin Porubský , Jiří Hodoň , Jarmila Stanková , Petr Džubák , Marián Hajdúch , Milan Urban , Jan Hlaváč

{"title":"Near-infrared pH-switchable BODIPY photosensitizers for dual biotin/cRGD targeted photodynamic therapy","authors":"Martin Porubský , Jiří Hodoň , Jarmila Stanková , Petr Džubák , Marián Hajdúch , Milan Urban , Jan Hlaváč","doi":"10.1016/j.jphotobiol.2024.113010","DOIUrl":"10.1016/j.jphotobiol.2024.113010","url":null,"abstract":"<div><p>Photodynamic therapy (PDT) is a clinically-approved cancer treatment that is based on production of cytotoxic reactive oxygen species to induce cell death. However, its efficiency depends on distribution of photosensitizer (PS) and depth of light penetration through the tissues. Tendency of pathological cancer tissues to exhibit lower pH than healthy tissues inspired us to explore dual-targeted pH-activatable photosensitizers based on tunable near-infrared (NIR) boron-dipyrromethene (BODIPY) dyes. Our BODIPY PSs were designed to carry three main attributes: (i) biotin or cRGD peptide as an effective cancer cell targeting unit, (ii) amino moiety that is protonated in acidic (pH <6.5) conditions for pH-activation of the PS based on photoinduced electron transfer (PET) and (iii) hydrophilic groups enhancing the water solubility of very hydrophobic BODIPY dyes. Illumination of such compounds with suitable light (>640nm) allowed for high phototoxicity against HeLa (α<sub>v</sub>β<sub>3</sub> integrin and biotin receptor positive) and A549 (biotin receptor positive) cells compared to healthy MRC-5 (biotin negative) cells. Moreover, no dark toxicity was observed on selected cell lines (>10 μM) providing promising photosensitizers for tumour-targeted photodynamic therapy.</p></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"259 ","pages":"Article 113010"},"PeriodicalIF":3.9,"publicationDate":"2024-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141978966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The synergistic effect of phototherapy and active substances on hair growth 光疗和活性物质对头发生长的协同作用

IF 3.9 2区生物学

Journal of photochemistry and photobiology. B, Biology Pub Date : 2024-08-10 DOI: 10.1016/j.jphotobiol.2024.113008

Shuting Qiu , Zhi Pan , Xiao Jiang , Guowen Lv , Anqi Feng , Hongbo Chen

{"title":"The synergistic effect of phototherapy and active substances on hair growth","authors":"Shuting Qiu , Zhi Pan , Xiao Jiang , Guowen Lv , Anqi Feng , Hongbo Chen","doi":"10.1016/j.jphotobiol.2024.113008","DOIUrl":"10.1016/j.jphotobiol.2024.113008","url":null,"abstract":"<div><p>Androgenic alopecia (AGA) typically manifests post-puberty, resulting in decreases in hair density, disruptions in the hair growth cycle, and alterations in hair follicle micro structure. Dihydrotestosterone (DHT) is a key hormone implicated in hair loss, especially on male. In this study, we found that each of arginine (Arg), arterial extract (AE) or biotin tripeptide-1 (BT-1), when combined with low level light therapy (LLLT, at 630 nm, 2 J/cm<sup>2</sup>), showed the efficacy in enhancing mitochondrial functions, cell proliferation and collagen synthesis in fibroblasts. Additionally, CARRIPOWER (the complexes of AE, BT-1, Arg, and Diaminopyrimidine derivatives), in conjunction with LLLT (630 nm, 2 J/cm<sup>2</sup>), showed promising results in dermal papilla cells (DPCs). The promising results contained not also inflammatory cytokines (IL-1β and IL-6) and cell pro apoptotic factor (TGF-β2) reduction, but also Wnt pathway inhibition by decreasing DKK1 level, and pro-hair growth factors (vascular endothelial growth factor (VEGF) and β-catenin) increase. This innovative combination therapy offers a potential solution for the treatment of AGA, addressing both hormonal and cellular factors involved in hair loss.</p></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"259 ","pages":"Article 113008"},"PeriodicalIF":3.9,"publicationDate":"2024-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141985134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The mechanisms of photoinhibition and repair in plants under high light conditions and interplay with abiotic stressors 强光条件下植物的光抑制和修复机制以及与非生物胁迫的相互作用

IF 3.9 2区生物学

Journal of photochemistry and photobiology. B, Biology Pub Date : 2024-08-09 DOI: 10.1016/j.jphotobiol.2024.113004

Fardad Didaran , Mojtaba Kordrostami , Ali Akbar Ghasemi-Soloklui , Pavel Pashkovskiy , Vladimir Kreslavski , Vladimir Kuznetsov , Suleyman I. Allakhverdiev

{"title":"The mechanisms of photoinhibition and repair in plants under high light conditions and interplay with abiotic stressors","authors":"Fardad Didaran , Mojtaba Kordrostami , Ali Akbar Ghasemi-Soloklui , Pavel Pashkovskiy , Vladimir Kreslavski , Vladimir Kuznetsov , Suleyman I. Allakhverdiev","doi":"10.1016/j.jphotobiol.2024.113004","DOIUrl":"10.1016/j.jphotobiol.2024.113004","url":null,"abstract":"<div><p>This review comprehensively examines the phenomenon of photoinhibition in plants, focusing mainly on the intricate relationship between photodamage and photosystem II (PSII) repair and the role of PSII extrinsic proteins and protein phosphorylation in these processes. In natural environments, photoinhibition occurs together with a suite of concurrent stress factors, including extreme temperatures, drought and salinization. Photoinhibition, primarily caused by high irradiance, results in a critical imbalance between the rate of PSII photodamage and its repair. Central to this process is the generation of reactive oxygen species (ROS), which not only impair the photosynthetic apparatus first PSII but also play a signalling role in chloroplasts and other cellulular structures. ROS generated under stress conditions inhibit the repair of photodamaged PSII by suppressing D1 protein synthesis and affecting PSII protein phosphorylation. Furthermore, this review considers how environmental stressors exacerbate PSII damage by interfering with PSII repair primarily by reducing de novo protein synthesis. In addition to causing direct damage, these stressors also contribute to ROS production by restricting CO<sub>2</sub> fixation, which also reduces the intensity of protein synthesis. This knowledge has significant implications for agricultural practices and crop improvement under stressful conditions.</p></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"259 ","pages":"Article 113004"},"PeriodicalIF":3.9,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141964540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Pyridinium fluorophore for the detection of zinc ions under autophagy conditions 用于自噬条件下锌离子检测的吡啶鎓荧光团。

IF 3.9 2区生物学

Journal of photochemistry and photobiology. B, Biology Pub Date : 2024-08-08 DOI: 10.1016/j.jphotobiol.2024.113006

Anagha Thomas , Anaga Nair , Sandip Chakraborty , Roopasree O. Jayarajan , Joshy Joseph , Ayyappanpillai Ajayaghosh

{"title":"A Pyridinium fluorophore for the detection of zinc ions under autophagy conditions","authors":"Anagha Thomas , Anaga Nair , Sandip Chakraborty , Roopasree O. Jayarajan , Joshy Joseph , Ayyappanpillai Ajayaghosh","doi":"10.1016/j.jphotobiol.2024.113006","DOIUrl":"10.1016/j.jphotobiol.2024.113006","url":null,"abstract":"<div><p>Molecular probes for sensing and imaging of various analytes and biological specimens are of great importance in clinical diagnostics, therapy, and disease management. Since the cellular concentration of free Zn<sup>2+</sup> varies from nanomolar to micromolar range during cellular processes and the high affinity Zn<sup>2+</sup> imaging probes tend to saturate at lower concentrations of free Zn<sup>2+</sup>, fluorescence based probes with moderate binding affinity are desirable in distinguishing the occurrence of higher zinc concentrations in the cells. Herein, we report a new, pentacyclic pyridinium based probe, <strong>PYD-PA,</strong> having a pendant <em>N,N</em>-di(pyridin-2-ylmethyl)amine (DPA) for Zn<sup>2+</sup> detection in the cellular environment. The designed probe is soluble in water and serves as a mitochondria targeting unit, whereas the pendent DPA acts as the coordination site for Zn<sup>2+</sup>. <strong>PYD-PA</strong> displayed a threefold enhancement in fluorescence intensity upon Zn<sup>2+</sup> binding with a 1:1 binding stoichiometry. Further, the probe showed a selective response to Zn<sup>2+</sup> over other biologically relevant metal ions with a moderate binding affinity (K<sub>a</sub> = 6.29 × 10<sup>4</sup> M<sup>−1</sup>), good photostability, pH insensitivity, and low cytotoxicity. The demonstration of bioimaging in SK-BR-3 breast cancer cell lines confirmed the intracellular Zn ion sensing ability of the probe. The probe was successfully applied for real time monitoring of the fluctuation of intracellular free zinc ions during autophagy conditions, demonstrating its potential for cellular imaging of Zn<sup>2+</sup> at higher intracellular concentrations.</p></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"259 ","pages":"Article 113006"},"PeriodicalIF":3.9,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141916919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photodynamic therapy with Photoditazine increases microviscosity of cancer cells membrane in cellulo and in vivo 使用光二氮嗪的光动力疗法可增加细胞和体内癌细胞膜的微粘度。

IF 3.9 2区生物学

Journal of photochemistry and photobiology. B, Biology Pub Date : 2024-08-08 DOI: 10.1016/j.jphotobiol.2024.113007

Liubov E. Shimolina , Aleksandra E. Khlynova , Aleksander A. Gulin , Vadim V. Elagin , Margarita V. Gubina , Pavel A. Bureev , Petr S. Sherin , Marina K. Kuimova , Marina V. Shirmanova

{"title":"Photodynamic therapy with Photoditazine increases microviscosity of cancer cells membrane in cellulo and in vivo","authors":"Liubov E. Shimolina , Aleksandra E. Khlynova , Aleksander A. Gulin , Vadim V. Elagin , Margarita V. Gubina , Pavel A. Bureev , Petr S. Sherin , Marina K. Kuimova , Marina V. Shirmanova","doi":"10.1016/j.jphotobiol.2024.113007","DOIUrl":"10.1016/j.jphotobiol.2024.113007","url":null,"abstract":"<div><p>Photodynamic therapy (PDT) is a minimally invasive method for cancer treatment, one of the effects of which is the oxidation of membrane lipids. However, changes in biophysical properties of lipid membranes during PDT have been poorly explored. In this work, we investigated the effects of PDT on membrane microviscosity in cancer cells in the culture and tumor xenografts. Membrane microviscosity was visualized using fluorescence lifetime imaging microscopy (FLIM) with a viscosity-sensitive rotor BODIPY2. It was found that PDT using chlorine e6-based photosensitizer Photoditazine caused a quick, steady elevation of membrane microviscosity both <em>in cellulo</em> and <em>in vivo</em>. The proposed mechanisms responsible for the increase in microviscosity was lipid peroxidation by reactive oxygen species that resulted in a decrease of phosphatidylcholine and the fraction of unsaturated fatty acids in the membranes. Our results suggest that the increased microviscosity is an important factor that contributes to tumor cell damage during PDT.</p></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"259 ","pages":"Article 113007"},"PeriodicalIF":3.9,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141975939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0