Journal of photochemistry and photobiology. B, Biology最新文献

{"title":"The integrated treatment of photodynamic therapy and oncolytic Virotherapy: A dual-modal paradigm for breast Cancer","authors":"Yixiang Li ,&nbsp;Kaidi Wang ,&nbsp;Qianhao Min,&nbsp;Hongyu Lu,&nbsp;Guoqing Zhang,&nbsp;Jianhua Yan","doi":"10.1016/j.jphotobiol.2025.113248","DOIUrl":"10.1016/j.jphotobiol.2025.113248","url":null,"abstract":"<div><div>Triple-negative breast cancer (TNBC) is a serious threat to lives and health. We developed a dual approach of Photodynamic therapy (PDT) and Newcastle Disease Oncolytic Virus (NDV) to mediate killing effects and anti-tumor immune effects against TNBC. In this study, we firstly verified that PDT combined with NDV effectively eliminated tumor cells. Cloning formation analysis, western blot and flow cytometer demonstrated that PDT + NDV triggered tumor cells ROS stress, apoptosis and inhibition of proliferation. Moreover, PDT + NDV upregulated the IL-6, STAT3 and NF-κB, increased the expression of HMGB1 and TNF-α, triggerd immunogenic cell death and strengthened the pro-inflammatory microenvironment, thereby effectively activating anti-tumor immune responses and enhancing the infiltration of CD8⁺ T cells. Our research demonstrated the synergistic anti-tumor effects of combining PDT and NDV, offering a promising strategy for the treatment of TNBC.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113248"},"PeriodicalIF":3.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144934097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Restoration of carious dentin using diode irradiation on unmodified and chitosan nanoparticle-modified adhesive before LED polymerization: A SEM, FTIR, and μTBS evaluation.","authors":"Amer M. Alanazi ,&nbsp;Shaheryar Shafqat ,&nbsp;Muhammad Omar Niaz ,&nbsp;Zainab Dua","doi":"10.1016/j.jphotobiol.2025.113265","DOIUrl":"10.1016/j.jphotobiol.2025.113265","url":null,"abstract":"<div><h3>Aim</h3><div>To investigate the restoration of caries-affected dentin (CAD) using adhesive modification technique via diode laser (DL) irradiation of unmodified and modified adhesive with Chitosan nanoparticles (CHNP) before light emitting diode (LED) polymerization, and CHNPs modified adhesive+LED polymerization without DL on micro-tensile bond strength (μTBS), Degree of conversion (DC) and resin tag length (RTL).</div></div><div><h3>Material and methods</h3><div>Forty-four human mandibular molars were obtained, and CAD was exposed according to ICDAS criteria 5. Teeth were positioned vertically within the auto-cure acrylic resin and randomly distributed into four categories according to the adhesive modification (<em>n</em> = 11). Group1-Etch(E) + Bond(B) + LED,Group 2-E + B(CHNP) + LED, Group 3-E + B + DL + LED and Group 4-E + B(CHNPS) + DL + LED. All samples were restored using composite buildup followed by thermal aging. Surface characterization of CHNP with Energy-dispersive spectroscopy (EDS) was assessed along with RTL via scanning electron microscopy (SEM). μTBS and failure modes were analyzed utilizing a universal testing machine (UTM) and stereomicroscope, respectively. The DC was quantified using a Fourier Transform Infrared Spectroscopy (FTIR). A one-way analysis of variance, complemented by Tukey's post hoc test, was implemented for comparisons among the investigated groups (<em>p</em> &lt; 0.05).</div></div><div><h3>Results</h3><div>Group 4-E + B(CHNP) + DL + LED (<em>107.43 ± 1.55</em> μ<em>m) presented the longest RTL with a score of grade 3 and the highest bond strength</em> (<em>11.54 ± 0.37 MPa). Whereas,</em> Group 1 (E + B+ LED) displayed the shortest RTL (<em>76.63 ± 0.82</em> μ<em>m) and lowest</em> μTBS <em>(9.31</em> ± <em>0.17 MPa).</em> The adhesive irradiated with DL before LED polymerization in Groups 2 and 4 presented comparable DC (p˃0.05).</div></div><div><h3>Conclusion</h3><div>Pre-irradiating both unmodified and modified adhesives with chitosan nanoparticles using a diode laser before LED polymerization seems to be an effective approach. This technique enhances the material's mechanical properties by improving adhesive penetration, which in turn affects the length of resin tags,μTBS, and DC.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113265"},"PeriodicalIF":3.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light-emitting diode-derived blue light overexposure accelerates corneal endothelial cell aging by inducing abnormal ROS accumulation","authors":"Zhaolin Liu ,&nbsp;Yiran Yang ,&nbsp;Ke Yan ,&nbsp;Jieli Wu ,&nbsp;Xiang Lin ,&nbsp;Liying Tang ,&nbsp;Qingjian Li ,&nbsp;Caihong Huang ,&nbsp;Jiaoyue Hu ,&nbsp;Zuguo Liu","doi":"10.1016/j.jphotobiol.2025.113252","DOIUrl":"10.1016/j.jphotobiol.2025.113252","url":null,"abstract":"<div><div>Blue light, defined as short-wavelength visible light ranging from 400 to 500 nm, is recognized for its high energy within the visible light spectrum. The prevalent use of light-emitting diodes (LEDs) has significantly increased exposure to blue light. Corneal endothelial cells (CECs) playing a crucial role in maintaining corneal transparency to get clear visual field. However, the specific effects of blue light on corneal endothelium remain unclear. To investigate this, in vivo and in vitro models of LED blue light irradiation were established. We examined changes in CEC fate and indicators related to oxidative stress. Our findings revealed that blue light exposure led to increased production of ROS in CECs, causing oxidative stress primarily in mitochondria. This, in turn, resulted in cell senescence, dysfunction, and apoptosis, ultimately contributing to the aging of corneal endothelium with accelerated cell loss. Notably, the rise in ROS levels triggered the activation of the Nrf2 signaling pathway in the early stages. This activation was associated with protective effects on CECs and inhibition of cell senescence. Our study sheds light on the intricate relationship between blue light exposure, oxidative stress, and the fate of CECs, providing valuable insights into the potential mechanisms underlying corneal aging.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113252"},"PeriodicalIF":3.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145005075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neodymium-doped yttrium vanadate laser and Phthalocyanine photosensitizer doped chitosan nanoparticle activated via Photodynamic therapy on smear layer and push-out bond strength of fiber post: An in vitro SEM, EDX assessment","authors":"Fahad Alkhudhairy","doi":"10.1016/j.jphotobiol.2025.113263","DOIUrl":"10.1016/j.jphotobiol.2025.113263","url":null,"abstract":"<div><h3>Aims</h3><div>To quantify the effectiveness of different root canal sterilants, i.e., Sodium Hypochlorite (NaOCl), Neodymium-doped Yttrium Vanadate (Nd: YVO₄) laser, Phthalocyanine photosensitizer doped Chitosan nanoparticles activated via Photodynamic therapy (Pc-CNPs-PDT), on smear layer (SL) elimination and push out bond strength (PBS) of glass fiber posts (GFPs) bonded to root dentin.</div></div><div><h3>Materials and methods</h3><div>Forty-eight human premolars with an approximate root length measuring 15 ± 1 mm were included. Endodontic treatment was performed using a nickel‑titanium rotary instrument followed by obturation. The post space was prepared, and specimens were allocated to four groups based on the disinfection protocol employed. Group 1 (Saline + EDTA), Group 2 (2.25 % NaOCl + EDTA), Group 3 (Nd: YVO4 laser + EDTA), Group 4 (Pc-CNPs-PDT + EDTA). A scanning electron microscope was used to assess the surface topography of CHNPs, energy-dispersive spectroscopy (EDS), and SL removal efficacy. Each post was luted using a dual-cure resin cement. The bonded specimens were sectioned into slices (coronal, middle, and apical). A universal testing machine and a stereomicroscope were used to test the PBS and failure modes, respectively. The dataset underwent analysis through one-way analysis of variance ANOVA and Tukey's test to determine notable differences among groups (<em>p</em> = 0.05).</div></div><div><h3>Results</h3><div>Coronal section of Group 4 (Pc (CHNPs)-PDT + EDTA) samples exhibited maximum SL removal. Whereas, minimum SL elimination was displayed by Saline pretreated canals at the apical section. Samples irrigated with 2.5 % NaOCl + EDTA and Pc (CHNPs)-PDT + EDTA presented comparable scores of bond integrity at all three root sections.</div></div><div><h3>Conclusion</h3><div>Phthalocyanine photosensitizer doped Chitosan nanoparticles, activated via Photodynamic therapy, can be considered as a suitable alternative to the gold standard technique, NaOCl and EDTA, in removing smear layer and improving glass fiber post adhesion to canal dentin.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113263"},"PeriodicalIF":3.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145061208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Near-IR quantum dot (BagQD) as fluorescent sensor for live-cell imaging and in-vivo antibacterial photocatalytic therapy (APT)","authors":"Subhendu Sekhar Bag ,&nbsp;Sayantan Sinha ,&nbsp;Aniket Banerjee ,&nbsp;Hemanshu Mediboyana ,&nbsp;Animes K. Golder","doi":"10.1016/j.jphotobiol.2025.113271","DOIUrl":"10.1016/j.jphotobiol.2025.113271","url":null,"abstract":"<div><div>The development of multitasking agents that can sense, as well as damage multidrug-resistant (MDR) bacteria with no growth, is the need of the hour to overcome the increased mortality associated with antibacterial resistance. Toward this end, to overcome the limitation of organic dyes as fluorescence probes, and other developed quantum dots (QD), we present new of its kind near infrared type-I (NIR-I) CdSe/ZnSe/FeTiO₃-based perovskite quantum dot, ^{<strong><em>Bag</em></strong>}<strong><em>QD</em></strong>, for fluorescence-guided selective bacterial imaging-sensing and permanent disinfection. ^{<strong><em>Bag</em></strong>}<strong><em>QD</em></strong> possesses unique photodynamic and photophysical properties of the near-infrared NIR-I biological window. The excellent electronic transitions of ^{<strong><em>Bag</em></strong>}<strong><em>QD</em></strong> resulted in efficient quantitative fluorescent sensing-imaging of gram-positive (G+ve) as well as gram-negative (G-ve) bacterial pathogens, and complete in-situ and in-vivo disinfection/elimination of pathogenic bacteria without regrowth via damaging bacterial cell wall and cellular DNA, by ROS generation during photocatalysis. The material cytotoxicity study showed no harm to the human cell line. Moreover, the therapeutic application of ^{<strong><em>Bag</em></strong>}<strong><em>QD</em></strong> on in-vivo mice skin wound infection, revealed the generation of hair follicles and epithelization within 6–8 days. Thus, ^{<strong><em>Bag</em></strong>}<strong><em>QD</em></strong> serves as a new alternative to antibiotics and a smart nanomedicine that can uphold the hope in revolutionizing diagnostics and clinical avenues.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113271"},"PeriodicalIF":3.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145220751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mimicking exogenous amino acids: Raman-based and machine learning tracking of Colon cancer cells metabolism","authors":"Monika Kopeć,&nbsp;Karolina Beton-Mysur,&nbsp;Arkadiusz Jarota,&nbsp;Beata Brożek-Płuska","doi":"10.1016/j.jphotobiol.2025.113285","DOIUrl":"10.1016/j.jphotobiol.2025.113285","url":null,"abstract":"<div><div>Amino acids are fundamental biomolecules that constitute the building blocks of proteins. Monitoring their dysregulation and fluctuations serves as an important indicator of human health. The aim of this study is to explore the role of exogenous amino acids metabolism in colon cancer development. Raman spectroscopy and Raman imaging were employed to investigate biochemical changes in normal human colon cells (CCD-18Co) and cancerous colon cells (Caco-2), both untreated and supplemented with leucine, threonine, and arginine. Spectroscopic data were analyzed using chemometric methods, specifically Partial Least Squares Discriminant Analysis (PLS-DA). Raman spectroscopy, combined with chemometric approach, identified unique Raman biomarkers – 1088/1262, 1444/1660, 1580/1004, and 1630/1444 - that correspond to changes in nucleic acids, lipids/lipids+Amide <em>I</em>, proteins, and lipids, respectively, in both normal and cancer cells supplemented with amino acids. These findings underscore the significant potential of Raman spectroscopy and Raman imaging as powerful tools for investigating the role of exogenous amino acids in colon cancer progression. We have also shown that the S₁-state lifetime of tetra-sulphonated aluminum phthalocyanine remains unaffected by amino acid supplementation, as confirmed by femtosecond transient absorption spectroscopy, supporting its stability as a photosensitizer for photodynamic therapy.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113285"},"PeriodicalIF":3.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145362162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of near-infrared-triggered photothermal therapy on collagen organization and wound healing","authors":"Yan Chen ,&nbsp;Chuanjie Zhang ,&nbsp;Xiuhong Weng ,&nbsp;Shengjun Jiang ,&nbsp;Bo Cheng","doi":"10.1016/j.jphotobiol.2025.113273","DOIUrl":"10.1016/j.jphotobiol.2025.113273","url":null,"abstract":"<div><div>Soft tissue wounds result in the loss of essential functions, including sensation and barrier protection. Mild photothermal therapy (PTT) has emerged as a promising therapeutic strategy for promoting wound healing and tissue regeneration, primarily through its ability to precisely regulate tissue temperature and thereby influence cellular proliferation and differentiation. In this study, gold nanorods (AuNRs) were employed as photosensitizers and co-cultured with fibroblasts to evaluate the effects of mild PTT on collagen organization and wound healing under the irradiation of 808 nm near-infrared (NIR) laser. AuNRs-mediated photothermal stimulation raised the temperature of the culture medium from 18.0 °C to 37.8 °C within 7 min. This controlled thermal stimulation significantly enhanced fibroblast proliferation and led to a more random distribution of collagen fibers. Moreover, AuNR-mediated mild PTT promoted the differentiation of fibroblast into myofibroblasts and reduced scar tissue formation. These findings highlight the therapeutic potential of mild PTT in soft tissue regeneration by modulating fibroblast behavior and remodeling collagen architecture.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113273"},"PeriodicalIF":3.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intranasal nanophotosensitizer enables safe and broad-spectrum photodynamic inactivation of respiratory viruses","authors":"Sunmi Han ,&nbsp;Assel Seitkazina ,&nbsp;Jeongyun Heo ,&nbsp;Se-young Kim ,&nbsp;SuJin Kim ,&nbsp;Sung-Ho Jeon ,&nbsp;Yung Hun Park ,&nbsp;Taehun Lim ,&nbsp;Bohyeon Kim ,&nbsp;Shivani Rajoriya ,&nbsp;Quy Son Luu ,&nbsp;Youngbok Lee ,&nbsp;Yong-Deok Lee ,&nbsp;Honghwan Choi ,&nbsp;Won-Keun Kim ,&nbsp;Hyun Jik Kim ,&nbsp;Sehoon Kim","doi":"10.1016/j.jphotobiol.2025.113261","DOIUrl":"10.1016/j.jphotobiol.2025.113261","url":null,"abstract":"<div><div>The emergence of highly transmissible respiratory viruses, including SARS-CoV-2 and its variants, emphasizes the urgent need for safe, variant-agnostic, and self-administered antiviral strategies. Here, we present a nanotheranostic platform (MBSD) based on methylene blue nanoparticles stabilized with a primary fatty acid naturally found in human nasal mucosa, designed for intranasal photodynamic inactivation (PDI). This nanoformulation enhances cellular permeability and singlet oxygen generation at sites of viral infection through strategic ion pairing and micellization using clinically approved excipients. MBSD demonstrated superior uptake and intracellular singlet oxygen generation in human nasal epithelial cells compared to free methylene blue. Upon exposure to red light, PDI treatment with MBSD significantly reduced viral gene expression and infectivity across multiple RNA and DNA viruses—including influenza A, SARS-CoV-2 variants (B.1 and Delta), Zika, Vaccinia, and emerging paramyxoviruses—with sub-nanomolar to low-nanomolar EC₅₀ values. In murine models, a single intranasal MBSD-mediated PDI treatment attenuated disease progression, markedly reduced lung viral burden and inflammation, and improved survival outcomes. In addition, repeated PDI treatments showed no detectable toxicity to normal mucosal tissues, indicating a favorable safety profile. These findings highlight MBSD-mediated PDI as a clinically translatable, non-invasive nanomedicine strategy that offers broad-spectrum antiviral efficacy and mucosal safety, supporting its potential as a frontline theranostic intervention for early-stage management of respiratory virus outbreaks.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113261"},"PeriodicalIF":3.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145040557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A theoretical model for optimizing UVA/riboflavin crosslinking","authors":"Shaowu Sun ,&nbsp;Xiaona Liu ,&nbsp;Jiayi Dong ,&nbsp;Xiaoyu Zhao ,&nbsp;Chenxi Zhang ,&nbsp;Chenyan Wang ,&nbsp;Xiaolu Ma ,&nbsp;Yan Wang ,&nbsp;Xiaona Li ,&nbsp;Zhipeng Gao ,&nbsp;Weiyi Chen","doi":"10.1016/j.jphotobiol.2025.113272","DOIUrl":"10.1016/j.jphotobiol.2025.113272","url":null,"abstract":"<div><div>Ultraviolet-A and riboflavin (UVA/R) crosslinking has emerged as a valuable technique for ocular disease treatments. However, the exact influence of the oxygen and riboflavin on the crosslinking has not been fully explored so far. A kinetic model was developed to predict corneal/scleral stiffening effects under varying oxygen levels, irradiation intensities, and riboflavin solution concentrations during crosslinking at a fixed fluence. The optical properties of porcine sclera were determined by UV–Vis spectrophotometry. Monte Carlo method was employed to evaluate intrastromal light absorption of riboflavin. The roles of oxygen and riboflavin in the reaction mechanism were proposed based on the kinetic interactions of reactive species within the corneal/scleral stroma. Kinetic simulations indicated that increasing intrastromal oxygen concentration, driven by higher ambient oxygen levels, significantly improves crosslinking efficiency. The crosslinker formation rate reach its maximum when the intrastromal riboflavin concentration was approximately 0.245 %. Concentrations that are either too low or too high are adversely affect the formation of crosslinker. Optimal stiffening effects could be achieved by balancing oxygen availability, irradiation intensity, and riboflavin solution concentration. The model accuracy of oxygen prediction was verified by experimental results obtained from corneal crosslinking. Linear correlations were found between the model-predicted concentration of newly induced crosslinkers and the experimentally measured mechanical properties of both the cornea and sclera under various crosslinking protocols. Our study improved the prediction model by introducing accurate optical properties of the sclera. This proposed model provides a possibility for predicting the biomechanical crosslinking efficacy of the cornea/sclera, and may be used for optimizing UVA/R crosslinking protocols in customized treatment for ocular disease.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113272"},"PeriodicalIF":3.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145266708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wavelength-dependent photobiomodulation attenuates synovial inflammation in fibroblast-like synoviocytes and a collagenase-induced osteoarthritis model","authors":"Tianxiang Fan ,&nbsp;Peng Xia ,&nbsp;Safayet Ahmed ,&nbsp;Yuen H. Tsang ,&nbsp;Zhi Yao ,&nbsp;Deli Wang ,&nbsp;Ziyu Chen ,&nbsp;Lixiang Xue ,&nbsp;Dongquan Shi ,&nbsp;Bo Wang ,&nbsp;Chi Fai Cheung ,&nbsp;Marco Y.C. Pang ,&nbsp;Ye Li ,&nbsp;Siu Ngor Fu","doi":"10.1016/j.jphotobiol.2025.113276","DOIUrl":"10.1016/j.jphotobiol.2025.113276","url":null,"abstract":"<div><div>Photobiomodulation (PBM) using light-emitting diodes (LEDs) has demonstrated promising anti-inflammatory effects in musculoskeletal disorders. However, the optimal parameters for modulating synovitis and joint degeneration in osteoarthritis (OA) remain unclear. This study aimed to examine the wavelength- and dose-dependent effects of PBM on inflammatory responses in fibroblast-like synoviocytes (FLS) and evaluate the in vivo therapeutic efficacy of selected wavelengths in a murine model of collagenase-induced OA (CIOA). Primary rat FLS were stimulated with TNF-α and irradiated using PBM at 625, 810, 940, or 1050 nm with fluences ranging from 13 to 78 J/cm² (fixed irradiance of 44 mW/cm²). mRNA levels of IL-1β, IL-6, COX-2, and iNOS were quantified by RT-qPCR. The in vivo study involved daily PBM (810 or 940 nm at 39 J/cm²) for two weeks in CIOA mice, starting one week post-induction. Pain-related behavior was assessed weekly using the incapacitance test. Synovial inflammation and cartilage degradation were evaluated by histology and immunofluorescence staining for IL-1β and IL-6. Among tested parameters, 810 nm at 39 J/cm² showed the most potent suppression of inflammatory gene expression in FLS. In vivo, 810 nm PBM significantly improved pain-related behavior by week 4, reduced synovitis and cartilage damage, and downregulated IL-1β and IL-6 expression in synovium. While 940 nm PBM also demonstrated anti-inflammatory effects in vitro, it did not show significant efficacy in vivo. These findings suggest that PBM at 810 nm exerts stronger anti-inflammatory and chondroprotective effects in inflammatory OA compared with 940 nm, supporting its potential as a non-pharmacological intervention for inflammatory OA phenotypes.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113276"},"PeriodicalIF":3.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145266693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}