Journal of photochemistry and photobiology. B, Biology最新文献

{"title":"Mechanistic insights into temoporfin-based photodynamic therapy: Ferroptosis as a critical regulator under normoxic and hypoxic conditions in head and neck cancer","authors":"Tong-Hong Wang ,&nbsp;Li-Fang Chou ,&nbsp;Yen-Wen Shen ,&nbsp;Nan-Chin Lin ,&nbsp;Yin-Hwa Shih ,&nbsp;Tzong-Ming Shieh","doi":"10.1016/j.jphotobiol.2025.113165","DOIUrl":"10.1016/j.jphotobiol.2025.113165","url":null,"abstract":"<div><div>Temoporfin is a second-generation photosensitizer used in photodynamic therapy (PDT) for the clinical treatment of head and neck cancer. However, its role in inhibiting cancer cell viability under normoxic and hypoxic conditions remains unclear. The oral squamous cell carcinoma (OSCC) cell lines, SAS and OECM-1 were cultured under normoxic or hypoxic conditions to investigate temoporfin-based PDT-induced cell death and the underlying mechanisms. Cell viability was analyzed using the MTT assay. Intracellular reactive oxygen species (ROS) levels, cell apoptosis, intracellular ROS, iron levels, lipid peroxidation, and glutathione (GSH) levels were assessed by flow cytometry. The expression of proteins related to oxidative stress, apoptosis, autophagy, and ferroptosis was verified by western blotting. Results showed that increasing the temoporfin dose, absorption time, and illumination time was positively correlated with the inhibition of oral cancer cells. Hypoxic conditions attenuated the toxicity of temoporfin in cancer cells. OECM-1 cells were more sensitive to temoporfin than SAS cells. Temoporfin-based PDT-induced ROS exhibited similar trends to oxidative stress-inducing enzymes under both normoxic and hypoxic conditions and triggered cell autophagy and ferroptosis. Administration of the ferroptosis inhibitor BRD4770 under normoxic conditions reversed temoporfin-based PDT-induced reductions in glutathione peroxidase 4 (GPx4), increasing in light chain 3-II (LC3-II) and cleaved poly (ADP-ribose) polymerase (cleaved-PARP). This study confirms that hypoxia weakens the anticancer effects of temoporfin-based PDT and that ferroptosis plays a key role in temoporfin-based PDT-mediated cancer cell inhibition.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"267 ","pages":"Article 113165"},"PeriodicalIF":3.9,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143855086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid penetrating inactivation and disinfection through ice layers by light-condensed deep ultraviolet LED irradiation","authors":"Lucheng Yu ,&nbsp;Peng Shen ,&nbsp;Zefeng Lin ,&nbsp;Shini Yang ,&nbsp;Maowei Yang ,&nbsp;Yaxiao Lou ,&nbsp;Shiqiang Lu ,&nbsp;Feiya Xu ,&nbsp;Shengrong Huang ,&nbsp;Xueqiong Sun ,&nbsp;Jiamin He ,&nbsp;Huachun Wang ,&nbsp;Yanhua Zhang ,&nbsp;Canming Zhang ,&nbsp;Fawen Su ,&nbsp;Fuchun Xu ,&nbsp;Xiaohong Chen ,&nbsp;Ling Li ,&nbsp;Duanjun Cai ,&nbsp;Junyong Kang","doi":"10.1016/j.jphotobiol.2025.113154","DOIUrl":"10.1016/j.jphotobiol.2025.113154","url":null,"abstract":"<div><div>The longer survival of virus and bacteria under low temperature greatly accelerates the infection risk and duration. How to effectively and rapidly inactivate them beneath ice layer in cold chain logistics has been an important issue never being well studied. Here, we demonstrate a novel light condenser design of high power UVC-LED device for rapid penetrating inactivation through ice layers. With fish-scale, ellipsoidal and alumina coated condenser, a portable UVC (275 nm) LED device in ultrahigh light output power (&gt; 3 W) was fabricated in a narrowed propagation angle (&lt; 60^o). Such condensed light beams are able to overcome the interfacial refringence at the ice and bubble interfaces in different types of ice layers for enhancing the transmission rate. As a result, the penetrating UVC light could give rise to the effective necrosis and apoptosis influence as well as DNA/RNA destruction on cells. A 99.99 % inactivation rate of <em>Escherichia coli</em> and <em>Staphylococcus aureus</em> has been obtained through 5-mm-thickness opaque ice layer. By 1.5 s irradiation, SARS-COV-2 virus can be completely inactivated and disinfected. This work proved the strong power of UVC-LED for cold chain disinfection applications.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"267 ","pages":"Article 113154"},"PeriodicalIF":3.9,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143843254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of 254 nm ultraviolet C promoting ethanol-induced gastric injury healing - A potential physical factor therapy","authors":"Xiao Zhang ,&nbsp;Dong-mei Li ,&nbsp;Hong-long Chen ,&nbsp;Xi-sheng Lin ,&nbsp;Su-su Li ,&nbsp;Le Yu ,&nbsp;Nan Peng ,&nbsp;Li-kang Wang ,&nbsp;Na Wang ,&nbsp;Yue-ming Gao","doi":"10.1016/j.jphotobiol.2025.113164","DOIUrl":"10.1016/j.jphotobiol.2025.113164","url":null,"abstract":"<div><h3>Background</h3><div>Gastritis, gastric ulcer, and gastric bleeding are associated with gastric mucosal injury. Chemical drugs are the current mainstream method, but the limitations are also increasingly apparent. Accumulative basic and clinical evidence has confirmed that ultraviolet C (UVC) has a significant effect on improving superficial infectious inflammation, promoting the healing of injured wounds, mucous membranes and ulcers, and killing microorganisms. It is possible to explore a new treatment method by introducing UVC light into the stomach to treat the damaged gastric mucosa.</div></div><div><h3>Objective</h3><div>This study evaluated the efficacy of introducing 254 nm UVC into the stomach by the self-made light guide at different doses and time to promote the healing of injured gastric mucosa, and explored the potential mechanism underling the treatment effect.</div></div><div><h3>Methods</h3><div>The rat model of gastric mucosal injury induced by ethanol was intervened by UVC with different radiation doses. The ulcer index, histopathology, CAT, SOD, MDA, NO, TNF-α, IL-6 and EGF in gastric mucosa and serum were evaluated. In addition, the expression of ASC, IL-1β and Caspase-1 in gastric tissue was evaluated.</div></div><div><h3>Results</h3><div>UVC significantly improved the histopathological condition of damaged gastric mucosa and reduce the ulcer index. UVC reduced the levels of TNF-α and IL-6, increase the level of EGF in serum. It also significantly increased the levels of SOD, CAT and NO, and reduced the levels of MDA in serum and gastric tissue. The expression of ASC, IL-1β and Caspase-1 in gastric mucosa was significantly inhibited, and it showed a significant dose-dependent trend with UVC irradiation dose.</div></div><div><h3>Conclusion</h3><div>Intragastric irradiation of 254 nm UVC can effectively promote the repair of damaged gastric mucosa by inhibiting inflammatory factors, activating antioxidant system and inhibiting the apoptosis-related proteins, which may provide a promising way for clinical application of physical factors in the treatment of gastric mucosal injury. However, whether the technology can be finally applied to clinical practice requires more rigorous safety and effectiveness studies.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"267 ","pages":"Article 113164"},"PeriodicalIF":3.9,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Collagen type III resists UVB induced photoaging of HaCaT cells by regulating mTOR signaling pathway","authors":"Helei Liu ,&nbsp;Hao Li ,&nbsp;Pengwei Zhao ,&nbsp;Rina Du ,&nbsp;Yaoxing Gao","doi":"10.1016/j.jphotobiol.2025.113153","DOIUrl":"10.1016/j.jphotobiol.2025.113153","url":null,"abstract":"<div><h3>Background</h3><div>Ultraviolet (UV) is the main factor leading to skin photoaging. In the process of photoaging, the dynamic balance of extracellular matrix is broken, resulting in the increase of reactive oxygen species (ROS), the accumulation of matrix metalloproteinases (MMPs), the decrease of collagen synthesis and the increase of degradation, and the skin becomes loose and wrinkled and pigmented, eventually leading to skin aging.</div></div><div><h3>Objective</h3><div>To investigate the effect of collagen type III (COLIII) on the autophagy level of human immortalized keratinocytes (HaCaT) induced by ultraviolet B (UVB) and explore whether COLIII can inhibit skin photoaging by changing the autophagy level.</div></div><div><h3>Methods</h3><div>HaCaT cells were irradiated with UVB to establish a photoaging model, and then the cell migration and repair ability, oxidative stress and inflammation levels, and autophagy levels were detected to explore the effect and mechanism of COLIII on autophagy in photoaged HaCaT cells.</div></div><div><h3>Results</h3><div>UVB radiation inhibited the cell proliferation and migration repair ability, resulting in the increase of ROS, inflammatory factors IL-6 and MMP-1 in HaCaT cells, while COLIII could attenuate the phototoxicity caused by UVB radiation and resist photoaging. In addition, COLIII can affect the level of autophagy through mTOR signaling pathway and protect HaCaT cells from UVB induced cytotoxicity, thereby attenuating light damage.</div></div><div><h3>Conclusion</h3><div>COLIII inhibits the autophagy level of UVB irradiated HaCaT cells through mTOR pathway, so as to combat UVB induced skin photoaging.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"267 ","pages":"Article 113153"},"PeriodicalIF":3.9,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LED photobiomodulation reduces myonecrosis and hemorrhage caused by PI metalloproteinase isolated from Bothrops jararacussu venom","authors":"Alex Augusto Ferreira e Ferreira ,&nbsp;Hallison Mota Santana ,&nbsp;Valdison Pereira dos Reis ,&nbsp;João Gabriel dos Santos Magalhães ,&nbsp;Milena Daniela Souza Silva ,&nbsp;Carolina Pereira da Silva ,&nbsp;Mauro Valentino Paloschi ,&nbsp;André Alves Silva ,&nbsp;Marlei Novaes de Sousa ,&nbsp;Andreimar Martins Soares ,&nbsp;Stella Regina Zamuner ,&nbsp;Juliana Pavan Zuliani","doi":"10.1016/j.jphotobiol.2025.113163","DOIUrl":"10.1016/j.jphotobiol.2025.113163","url":null,"abstract":"<div><div><em>Bothrops jararacussu</em> is one of the species most frequently involved in snakebite incidents. The metalloproteinase, which constitutes 26.2 % of the venom composition of this species, is a key factor responsible for severe tissue damage, including hemorrhage and myonecrosis. While antivenom treatment effectively addresses systemic effects, its efficacy in mitigating local damage remains limited. In this context, the present study aimed to investigate the effects of photobiomodulation using a 945 nm LED following experimental envenomation with a P<img>I class metalloproteinase, BjussuMP-II, isolated from <em>B. jararacussu</em> venom. Experimental envenoming was induced in male Swiss mice (18–22 g) after an injection of BjussuMP-II (50 μg) or PBS (50 μL) into the gastrocnemius muscles or dorsal skin. After 30 min, treatments with antivenom, LED, or a combination of both were administered. Three hours later, blood and muscle samples were collected for myotoxicity and histological analyses, and the dorsal skin was excised for hemorrhagic halo analysis. Results demonstrate that antivenom treatment alone is insufficient to mitigate the effects caused by BjussuMP-II, highlighting its ineffectiveness against the local damage induced by snakebite envenomation. In contrast, LED photobiomodulation, both as a standalone treatment and in combination with antivenom, effectively reduced myotoxicity, tissue damage, and hemorrhage induced by BjussuMP-II, both in the muscle and dorsal skin. In conclusion, LED treatment significantly reduces myotoxicity, tissue damage, and hemorrhage when applied independently. The combined application of antivenom and LED was also equally effective in mitigating these effects, demonstrating an advantage in the association of these two resources, as antivenom is essential for the reversal of systemic damage.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"267 ","pages":"Article 113163"},"PeriodicalIF":3.9,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Suppression of NRF2 by photodynamic action to enhance bortezomib-triggered DNA damage for synergistic colorectal cancer therapy","authors":"Liu-Gen Li ,&nbsp;Hao Chen ,&nbsp;Ting-Ting Yu ,&nbsp;Rui-Lin Liu ,&nbsp;Cunqing Kong ,&nbsp;Fan Leng ,&nbsp;Rong-Cheng Xiao ,&nbsp;Yao-Hua Lu ,&nbsp;Ning Han ,&nbsp;Jun Hu ,&nbsp;Yuan-Jian Hui ,&nbsp;Tong-Fei Li","doi":"10.1016/j.jphotobiol.2025.113162","DOIUrl":"10.1016/j.jphotobiol.2025.113162","url":null,"abstract":"<div><div>Anti-tumor agent bortezomib (BTZ) showed poor efficacy in colorectal cancer (CRC), which is attributed to the high expression of KEAP1-NRF2, an essential anti-oxidative system. Oxidative DNA damage is one of the critical mechanisms by which photodynamic therapy (PDT) induces apoptosis in tumor cells, which can be considered an important tool for early non-invasive treatment of CRC. The present work aims to explore the role and mechanism of PDT synergizing with BTZ in the treatment of CRC. The findings showed slight decrease in cell viability in BTZ-treated CRC cells of SW480 and SW620. KEAP1-NRF2 was highly expressed in CRC as revealed by GEPIA, TIMER databases, and in vitro experiments, conferring antioxidant activity. Further investigation revealed BTZ bound KEAP1, resulting in KEAP1-NRF2 decoupling, which was manifested as downregulation of KEAP1 and upregulation of NRF2. CO-IP test provides in-depth confirmation that BTZ impaired NRF2 ubiquitination. When SW480 and SW620 cells were co-treated with the chlorin e6 (Ce6)-PDT and BTZ, the expression of NRF2 was reduced with increased ROS generation and enhanced DNA damage. The combination of PDT and BTZ exhibited robust anti-CRC efficacy, characterized by increased apoptosis in SW480 and SW620 cells and dramatic inhibition of tumor growth in SW480 cell-bearing mice. To summarize, Ce6-mediated PDT combined with BTZ suppresses NRF2 and thus enhances DNA damage against CRC. The present study uncovered the molecular mechanism of redox in CRC and recommends an effective intervention strategy with PDT.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"267 ","pages":"Article 113162"},"PeriodicalIF":3.9,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"aPDT activity of new water soluble phenalenone derivatives with shifted UV–Vis absorption","authors":"C. Silva ,&nbsp;Y. Vásquez-Martínez ,&nbsp;C. Leal-Carmona ,&nbsp;Y.H. Cho ,&nbsp;M. Carrasco-Bozo ,&nbsp;C. Sandoval-Altamirano ,&nbsp;J. Morales ,&nbsp;G. Gunther","doi":"10.1016/j.jphotobiol.2025.113152","DOIUrl":"10.1016/j.jphotobiol.2025.113152","url":null,"abstract":"<div><div>Antibacterial therapy is one of the most important medical developments of the 20th century, but several decades of antibiotic misuse and abuse have created a health emergency. Antibiotics resistance and ineffectiveness have spread through the community, threatening the enormous gains made by the availability of therapies.</div><div>The emergence of drug-resistant infections has encouraged research community to develop new mechanisms against bacterial infections, mainly focused on multi-target strategies like “Antibacterial Photodynamic Therapy (aPDT)”, where singlet oxygen (an excited state of oxygen) is able to oxidize a wide range of biomolecules including proteins, lipids and nucleic acids, leading to bacterial death. Singlet oxygen oxidative damage in aqueous media is restricted by its short diffusion range, around 150 nm. It is crucial to increase photosensitizer solubility in aqueous media keeping the capability of partition in apolar media (like membranes).</div><div>We have previously demonstrated that an alkoxy substitution in position 6 of phenalenone scaffold (6-alkoxy-PNF) promotes a bathochromic shift of UV–Vis absorption when compared to clean phenalenone, (with maximum absorption wavelength centered at around 430–450 nm depending on the solvent). Their quantum yields of singlet oxygen generation remained high, in most media.</div><div>To take advantage of the photo-physical properties of 6-alkoxy-PNF framework, increase solubility in water and promote attractive electrostatic interaction on the bacterial surface, a trimethylammonium group was introduced in the molecule. Moreover, depending on the length of methylene chain included, hydrophilic lipophilic balance of molecules can be tuned. This substitution through a methylene linker would maintain distance from the 6-alkoxy-PNF, keeps almost unchanged their visible absorption bands (displaced to the red) and their singlet oxygen generation capacity.</div><div>Their ability to generate singlet oxygen and hence inactivate bacteria was tested. Our results show that the behavior of this family of compounds is dependent on the length of the alkyl chain, particularly in micro-heterogeneous systems. Synergic effect can be attributed to 12C surfactant associated with antimicrobial surfactant ability and singlet oxygen generation.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"267 ","pages":"Article 113152"},"PeriodicalIF":3.9,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143769196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the phototherapy modalities and dosages for an ingestible light-emitting diode capsule to eliminate Helicobacter pylori infection","authors":"Jiashen Hu ,&nbsp;Xiafei Shi ,&nbsp;Shisheng Cao ,&nbsp;Xiaoxi Dong ,&nbsp;Jianwu Dai ,&nbsp;Huijuan Yin","doi":"10.1016/j.jphotobiol.2025.113155","DOIUrl":"10.1016/j.jphotobiol.2025.113155","url":null,"abstract":"<div><div><em>Helicobacter pylori</em> (<em>H. pylori</em>) infection presents increasing challenges to antibiotic therapies owing to limited drug bioavailability, multi-drug resistance and collateral damage to commensal intestinal microflora. To address these problems, here, an ingestible magnetically controlled light-emitting diode (LED) light source was designed for an ingestible capsule to perform antimicrobial photodynamic therapy (aPDT) without an exogenous photosensitizer (ex-PS) at 630 nm. Specifically, we first optimized the antibacterial rates of aPDT with ex-PS and aPDT without ex-PS against <em>H. pylori</em> at the bacterial suspension level by varying the wavelength (405, 530, 630 nm), photosensitizer concentration (2, 4, 6, 8, 10 μg/mL), power density (15, 30 mW/cm²), and energy density (0, 3.6, 7.2, 10.8, 14.4, 18.0 J/cm²). Then, we compared the antibacterial effect of aPDT with ex-PS and aPDT without ex-PS against <em>H. pylori</em> at the biofilm level, revealing that the antibacterial rate of aPDT without ex-PS reached approximately 97 % at 405 nm and 18 J/cm², similar to that of aPDT with ex-PS under the same conditions. Furthermore, 80 SD rats infected with <em>H. pylori</em> were treated with aPDT with ex-PS and aPDT without ex-PS at the above wavelengths. Histopathological analysis of rat gastrointestinal tissues revealed that aPDT with ex-PS and aPDT without ex-PS exhibited significant antibacterial activity against <em>H. pylori</em>, without side effects on normal tissues. Additionally, aPDT without ex-PS at 630 nm induced an anti-inflammatory response and regulated the intestinal flora. Ultimately, we developed a magnetically controlled LED capsule for in vivo aPDT without ex-PS at 630 nm against <em>H. pylori</em>.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"267 ","pages":"Article 113155"},"PeriodicalIF":3.9,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143769197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photobiomodulation therapy induces NG2 activation through dermal adipocyte lipolysis during wound healing","authors":"Ivana Márcia Alves Diniz ,&nbsp;Rafaela Férrer de Oliveira ,&nbsp;Isabella Bittencourt do Valle ,&nbsp;Caroline Carvalho Picoli ,&nbsp;Humberto Jácome-Santos ,&nbsp;Luiza de Almeida Queiroz Ferreira ,&nbsp;Gleide Fernandes Avelar ,&nbsp;Marina Gonçalves Diniz ,&nbsp;Alexander Bibrair","doi":"10.1016/j.jphotobiol.2025.113151","DOIUrl":"10.1016/j.jphotobiol.2025.113151","url":null,"abstract":"<div><div>Photobiomodulation therapy (PBMT) is a rapidly advancing approach for restoring damaged tissues, particularly in skin and mucosal wounds. While its application is promising, the role of mature adipocytes in regenerating mesenchymal tissues after PBMT remains largely unexplored. This study demonstrates that PBMT applied to skin wounds significantly reduces the number and size of mature adipocytes. Additionally, PBMT modulates the upregulation of <em>peroxisome proliferator-activated receptor γ</em> (<em>PPARγ</em>), increasing the gene expression of <em>fatty acid binding protein 4</em> (<em>Fabp4</em>) and <em>perilipin 1</em>, which are linked to enhanced lipolysis. The molecular activation of <em>neural/glial antigen 2</em> (<em>NG2</em>) indicates the recruitment of progenitor cells following mature adipocytes lipolysis. <em>In vitro</em>, PBMT improved dermal skin cell proliferation, migration, inflammatory regulation, and differentiation capacities. These findings reveal a novel mechanistic pathway for skin regeneration, emphasizing the therapeutic potential of PBMT in modulating dermal fat tissue to facilitate wound healing. Collectively, this emerging knowledge provides valuable insights into managing dermal fat tissue to support wound healing.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"267 ","pages":"Article 113151"},"PeriodicalIF":3.9,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143777423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and evaluation of nanocomposite vaginal rings for photodynamic therapy against Candida albicans","authors":"Sara Efigênia Dantas de Mendonça y Araújo ,&nbsp;Brenda Maria Silva Bezerra ,&nbsp;Mariana Rillo Sato ,&nbsp;Rui Oliveira Macedo ,&nbsp;João Augusto Oshiro-Junior","doi":"10.1016/j.jphotobiol.2025.113149","DOIUrl":"10.1016/j.jphotobiol.2025.113149","url":null,"abstract":"<div><div>Antimicrobial photodynamic therapy (aPDT) is based on oxidative photochemical reactions and can be used against vulvovaginal candidiasis (VVC), caused by <em>Candida albicans</em>. This can be achieved by incorporating phthalocyanine (PC) into a polymer-matrix nanocomposite based on nanostructured lipid carriers (NLC) ureasil-polyether (UPEO), which allows them to be molded into a vaginal ring. The aim was to evaluate the aPDT of PC-NLC-UPEO rings against <em>C. albicans</em>. DLS and NTA showed values for NLC and PC-NLC within the nanocomposite's ideal range for pharmacological purposes. Microscopic analysis showed that NLC and PC-NLC have a spherical shape and PC-NLC-UPEO may promote better adhesion and drug release based on the roughness shown. NLC-UPEO and PC-NLC-UPEO were 0° of contact angle, demonstrating total wettability. The FTIR spectra showed no suppression of the characteristic groups of the materials and no new bands appeared. TG showed an improvement in the thermal stability of NLC when dispersed in UPEO. DTA revealed endothermic events near 100 °C related to solvent loss and dehydration. The PC released from PC-UPEO and PC-NLC-UPEO, around 37.8 % and 39.4 % were released within 24 h, which can promote initial antifungal activity. In the colony count method, PC and PC-NLC showed a reduction of 99 % in the analyses with light. Agar diffusion assay revealed halos of 7 and 15 mm in the groups with light, PC-UPEO, and PC-NLC-UPEO, respectively, demonstrating improved PC activity. The results highlight the potential of vaginal rings associated with aPDT to be administered to treat <em>C. albicans</em>.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"266 ","pages":"Article 113149"},"PeriodicalIF":3.9,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143610014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}