Journal of photochemistry and photobiology. B, Biology最新文献

{"title":"A wash-free red fluorescent probe for real-time monitoring of mitochondrial viscosity changes and tumor imaging","authors":"Kun Yu ,&nbsp;Yeming Zhang ,&nbsp;Yuqing Wang ,&nbsp;Jiale Li ,&nbsp;Jie Wang ,&nbsp;Sichen Zhang ,&nbsp;Aobo Sun ,&nbsp;Xue Zhang ,&nbsp;Lei Hu ,&nbsp;Hui Wang","doi":"10.1016/j.jphotobiol.2025.113182","DOIUrl":"10.1016/j.jphotobiol.2025.113182","url":null,"abstract":"<div><div>Mitochondria are important energy-producing organelles within cells, and abnormalities in the viscosity of their microenvironment are closely related to diseases such as cancer. Current methods for detecting viscosity still suffer from many limitations, whereas fluorescence imaging techniques can address these shortcomings. Therefore, there is an urgent need to develop fluorescence probes capable of detecting changes in mitochondrial viscosity. In this study, derivatives of triphenylamine were used as the core structure, and different triphenylphosphine derivatives were introduced through a condensation reaction to synthesize three red fluorescence probes with a D-π-A structure. Their photophysical properties have been systematically studied and it has been found that they are not only sensitive to viscosity but also exhibit strong anti-jamming capabilities. Among them, probe <strong>TPAP2</strong> exhibits excellent optical properties, including large Stokes shifts and high sensitivity to viscosity. It was found that <strong>TPAP2</strong> is mitochondria-targeted, washing-free, and fast (&lt;10s), with the targeting process depending on the mitochondrial membrane potential. The probe has been successfully applied to tumor imaging in mice with subcutaneous tumors.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"268 ","pages":"Article 113182"},"PeriodicalIF":3.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of various carbon supports for TiO2 and N-doped TiO2 photocatalysts for inactivation of Escherichia coli in water under UV–visible light","authors":"Mutcha Shanmukha Rao ,&nbsp;Ashish Tiwari ,&nbsp;Diptipriya Sethi ,&nbsp;Tapan Dash ,&nbsp;Kamatchi Jothiramalingam Sankaran ,&nbsp;Ramasamy Sakthivel","doi":"10.1016/j.jphotobiol.2025.113180","DOIUrl":"10.1016/j.jphotobiol.2025.113180","url":null,"abstract":"<div><div>This study evaluates the effectiveness of various carbon nanostructures carbon glassy spheres (CGS), carbon nanopowder (CNP), and activated carbon (AC) as supports for TiO₂ and N-TiO₂ photocatalysts in the inactivation of <em>Escherichia coli (E. coli)</em> in water. The photocatalysts were synthesized using a sol-gel method. Transmission electron microscopy (TEM) and X-ray diffraction (XRD) analyses confirmed the presence of anatase-phase TiO₂ on the carbon supports, with the synthesized TiO₂ nanoparticles exhibiting particle sizes below 10 nm. Fourier transform infrared (FTIR) spectroscopy identified metal‑oxygen bonds and surface hydroxyl groups on the photocatalyst surface, while X-ray photoelectron spectroscopy (XPS) confirmed the Ti⁴⁺ oxidation state and nitrogen incorporation in nitrogen-doped TiO₂ (N-TiO₂) samples. The photocatalytic performance of these materials was investigated for <em>E. coli</em> inactivation under both UV and UV–visible light irradiation. Results demonstrated that carbon-supported TiO₂ and N-TiO₂ nanocomposites exhibited significantly enhanced photocatalytic activity compared to their unsupported counterparts. Superior photocatalytic performance was observed under UV–visible light compared to UV light. Among the three carbon-supported systems, TiO₂ and N-TiO₂ nanocomposites deposited on CGS achieved the highest photocatalytic activity. The enhanced performance was attributed to the morphology and particle size of the carbon supports, which played critical roles in optimizing photocatalytic inactivation of <em>E. coli</em> in water.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"268 ","pages":"Article 113180"},"PeriodicalIF":3.9,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanobioactive glass/chitosan/collagen composite loaded with methylene blue for tissue regeneration and bacterial infection treatment by photodynamic therapy","authors":"Sarah M. Ali ,&nbsp;Elshimaa H. Gomaa ,&nbsp;Entsar E. Badr ,&nbsp;M.A. Azooz ,&nbsp;Mohammad M. Farag","doi":"10.1016/j.jphotobiol.2025.113179","DOIUrl":"10.1016/j.jphotobiol.2025.113179","url":null,"abstract":"<div><div>Development of suitable tissue engineering scaffolds to be loaded with photosensitizer drugs applied in photodynamic therapy is considered a critical issue. This study we introduced new delivery system based on cerium- and silver-doped nanobioactive glass (nBG)/chitosan/collagen composite scaffolds as a carrier for a photosensitizer, methylene blue (MB), in photodynamic therapy, capitalizing on the unique photocatalytic and antibacterial properties of the doped nBG. Cerium and silver offer additional benefits in terms of antimicrobial, anticancer, and wound healing properties. MB is an effective and inexpensive therapeutic photosensitizer compound that exhibits antimicrobial activity through light activation and has shown great potential for selectively treating infected cells. The prepared scaffolds were characterized by FTIR, TGA, and SEM/EDX. The bioactivity and biodegradation were conducted in SBF. The cell viability was carried out against the WI-38 cell line. Finally, the antibacterial efficiency of scaffolds loaded with MB was evaluated before and after laser irradiation against <em>Staphylococcus aureus, Bacillus subtilis (Gram-Positive),</em> and <em>Pseudomonas aerginousea, klebsiella pneumoniae (Gram-negative)</em>. Inhibition of bacteria by reactive oxygen species (ROS) was also measured. Furthermore the primarily MB release profile and kinetic were studied. The results showed that nBG particles increased the thermal stability of the scaffolds, and enhanced the formation of an apatite bone-like layer on the scaffold surfaces. Furthermore, scaffold degradation was tailored by changing the amount of nBG. Moreover, the water uptake of the scaffold containing undoped nBG particles (G010 and G020 samples) showed higher water uptake percentages (682 % and 762 %, respectively) than doped nBG (for 155 % and 322 % for GA10 and GA20, respectively). All scaffolds demonstrated good cell viability at concentrations up to 62.5 μg/ml. However, at higher concentrations, scaffolds incorporating high percentages of nBG were more viable than those containing low amounts of nBG, regardless of the glass composition. The antibacterial tests presented that the MB enhanced scaffold antibacterial efficiency by improving the oxidative stress on bacterial cells, which significantly increased after irradiation. Finally, the release mechanism of MB was diffusion mechanism for scaffolds contained nBG particles, while it did not follow this mechanism for blank polymer scaffolds. In conclusion, these results suggest that the developed multifunctional scaffolds hold significant promise for effective photodynamic antimicrobial chemotherapy in the treatment of microbial infections.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"268 ","pages":"Article 113179"},"PeriodicalIF":3.9,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemiluminescent and fluorescent properties of extracellular red pigment from Talaromyces purpureogenus","authors":"Sharad Bhatnagar ,&nbsp;Wai Lam Tse ,&nbsp;Haruhiko Yoneta ,&nbsp;Yoichi Nakao ,&nbsp;Hideki Aoyagi","doi":"10.1016/j.jphotobiol.2025.113178","DOIUrl":"10.1016/j.jphotobiol.2025.113178","url":null,"abstract":"<div><div><em>Talaromyces purpureogenus</em> is an attractive option for natural pigment production owing to its ability to produce large amounts of water-soluble extracellular red pigments. Recently, natural pigments have been used to develop halochromic biosensing applications, in which the colour change acts as a signal in response to a stimulus; however, the shelf-life and signal generation ability of packaging employing natural pigments maybe suboptimal because of potential reactions with environmental factors. Therefore, this study aimed to develop an alternative method for producing measurable signals from pigments. For this, the chemiluminescent and fluorescent properties of the pigments were explored. The pigment exhibited luminescence when exposed to hydrogen peroxide, which was affected by the reactant concentration, pH levels, and incubation time. Moreover, a rapid signal enhancement exceeding eight-fold was developed by adding luminol. The fluorescence spectrum was strongly affected by pH, with alkaline pH markedly increasing the fluorescence. Fluorescence was also found to be dependent on incubation time, with the maximum signal obtained at pH 12 and 72 h (∼16-fold increase over 0 h), and a model for the increase in fluorescence with time was developed (0.90 &lt; <em>R</em>² &lt; 0.99). The identification of pigment components confirmed the presence of atrorosin R, which had an isochromene/isoquinoline ring, and elucidated the planar structure of this pigment. These findings may aid in developing novel biosensors utilising fungal azaphilone pigments.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"268 ","pages":"Article 113178"},"PeriodicalIF":3.9,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143948476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effects of oral milk ceramide-collagen peptides mixtures in preventing UV-induced inflammation and photoaging through TGF-β and NF-κB/MAPK signaling pathways in UV-exposed hairless mice","authors":"Sung Hee Han ,&nbsp;Hyung Joo Suh ,&nbsp;Sang Jun Lee ,&nbsp;Yeok Boo Chang","doi":"10.1016/j.jphotobiol.2025.113171","DOIUrl":"10.1016/j.jphotobiol.2025.113171","url":null,"abstract":"<div><div>This study investigates the synergistic effects of oral milk ceramide-collagen peptides in inhibiting UV-induced inflammation and preventing photoaging. The optimal ratio of milk ceramide to collagen peptides was determined in HaCaT cells, and the effects of oral supplementation of milk ceramide-collagen peptides were evaluated in UV-exposed hairless mice. HaCaT cells did not exhibit cytotoxicity when treated with milk ceramide and collagen peptides at concentrations up to 200 μg/mL. UVB exposure decreased cell viability, but treatment with the milk ceramide-collagen peptides mixtures (1:1, 1:3) prevented further viability loss and improved collagen peptides synthesis markers, including MMPs and TIMPs. The combination also enhanced moisture-related factors (AQP3, FLG) and reduced inflammatory cytokines (IL-6, IL-1β) and COX expression. In hairless mice, oral supplementation of milk ceramide-collagen peptides mixture (1:1 ratio) improved skin hydration, reduced erythema, TEWL, skin thickness, and wrinkle formation in a dose-dependent manner. The treatment also suppressed the expression of MMPs and TIMPs, promoting collagen peptides synthesis. Furthermore, the mixtures regulated NF-κB and MAPK signaling pathways, reducing inflammation and photoaging. These results suggest that the 1:1 milk ceramide-collagen peptides mixture effectively prevents UV-induced skin damage and photoaging by enhancing collagen peptides production and improving skin barrier function.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"268 ","pages":"Article 113171"},"PeriodicalIF":3.9,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143902103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Binding of collagen I to integrins alleviates UVB-caused mitochondrial disorders in human keratinocytes HaCaT through enhancement of F-actin polymerization","authors":"Yuying Zhu ,&nbsp;Tianyu Ji ,&nbsp;Zhao San ,&nbsp;Ruiyi Cao ,&nbsp;Toshihiko Hayashi ,&nbsp;Kazunori Mizuno ,&nbsp;Shunji Hattori ,&nbsp;Hitomi Fujisaki ,&nbsp;Weiwei Liu ,&nbsp;Takashi Ikejima","doi":"10.1016/j.jphotobiol.2025.113170","DOIUrl":"10.1016/j.jphotobiol.2025.113170","url":null,"abstract":"<div><div>Collagen I is one of the major components of the extracellular matrix in human skin, and is frequently used in skin cares and medications. Previously, we revealed that human keratinocytes HaCaT cells grown on collagen I (Col)-coated dishes gain resistance against UVB damages owing to the restored mitophagy. In this study, we further investigate the mechanisms by which collagen I modulates mitophagy. UVB irradiation causes loss of integrin β1 and collapse of F-actin cytoskeleton. Considering the requirement of actin skeleton in various cellular processes, we are curious about the participation of F-actin collapse in UVB damage. Integrin β1, whose activation enhances F-actin assembly, is a potential target for Col in UVB-treated cells. Notably, inhibiting integrin by adding an inhibitor RGDS or siRNA attenuates the effect of Col against UVB damages, confirming the participation of integrin in cell protection. The collapse of F-actin is rescued by Col, accompanying increases in the mRNA of F-actin polymerization-associated proteins and decreases in the mRNA of depolymerization-associated proteins. Inhibiting actin polymerization by using cytochalasin D represses the protective effect of Col, confirming the cytoprotective role of F-actin in UVB-treated cells. Remarkably, mitophagy in UVB-treated cells restored by Col-coating is inhibited by adding cytochalasin D or RGDS, as shown by the decreases of lysosomes, mitochondrial ubiquitin proteins, and co-localization of autophagosomes and mitochondria, resulting in accumulation of damaged mitochondria, which stresses the importance of F-actin and integrin in mitophagy. In summary, integrins and F-actin are required for mitophagy in UVB-irradiated HaCaT cells, and their enhancements by Col-coating facilitate timely elimination of damaged mitochondria caused by UVB, finally contributing to cell survival.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"268 ","pages":"Article 113170"},"PeriodicalIF":3.9,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143908233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating photosystem II efficiency in Parachlorella kessleri under atrazine exposure using chlorophyll a fluorescence analysis","authors":"Brian Ospina Calvo ,&nbsp;Ángela B. Juárez ,&nbsp;M. Gabriela Lagorio","doi":"10.1016/j.jphotobiol.2025.113167","DOIUrl":"10.1016/j.jphotobiol.2025.113167","url":null,"abstract":"<div><div>This study aims to evaluate the toxicity of Atrazine on freshwater algae by employing spectroscopic analysis (specifically the F₆₈₃/F₇₃₀ ratio) and photosynthetic parameters (Fv/Fm, Fv/F₀, ФC, ФPSII, qP, and UQF) as bioindicators. The unicellular alga <em>Parachlorella kessleri</em> was selected for this investigation. Cells of <em>Parachlorella kessleri</em> were cultivated under axenic conditions in Bold's Basal Medium (BBM) and subsequently exposed to various concentrations of Atrazine (260, 520, and 1040 μg/L) for three distinct exposure durations (1, 60, and 360 min). Reflectance, transmittance, basal steady-state emission spectra, and variable chlorophyll-<em>a</em> fluorescence were recorded and analyzed for both control and treated algae groups. The results demonstrated that Atrazine inhibits electron transport in the photosynthetic chain of <em>Parachlorella kessleri</em>, leading to a marked decline in photosynthetic efficiency. The absorbed light energy was predominantly dissipated via the physical de-excitation pathway (ΦC), while regulated heat dissipation remained minimal—even in the absence of the herbicide—suggesting a possible deficiency in the PGR5-dependent photoprotective pathway. Among the tested fluorescence parameters, ΦPSII, derived from Kautsky kinetics, proved to be the most sensitive to Atrazine exposure, highlighting its potential use in algae-based biosensors for herbicide detection in aquatic environments. Although the OJIP method provided a faster response and detected abrupt changes in relative chlorophyll fluorescence (VOP), these changes did not correlate with herbicide concentration. Steady-state fluorescence, especially when corrected for inner filter effects, may offer a complementary, non-invasive tool for monitoring aquatic stressors. However, anomalous responses at high herbicide concentrations indicated the need for further validation under varied conditions.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"267 ","pages":"Article 113167"},"PeriodicalIF":3.9,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological and biochemical analysis along with gene expression dynamics in Lilium ‘Brunello’ under supplemented effect of blue and red light treatment in pin tray soil-less cultivation system","authors":"Mahinder Partap ,&nbsp;H.N. Deekshith ,&nbsp;Himanshi Gupta ,&nbsp;Gulshan Birsanta ,&nbsp;Payal Kapoor ,&nbsp;Vikas Soni ,&nbsp;Bhavya Bhargava","doi":"10.1016/j.jphotobiol.2025.113168","DOIUrl":"10.1016/j.jphotobiol.2025.113168","url":null,"abstract":"<div><div>The present research corroborates the influence of supplementation effect of blue light (BL), red light (RL), and the combination of both BL + RL on the morphological traits, biochemical, and expression of key flowering genes assessment in lilium cultivated in bulb forcing soil-less pin trays within a climate-controlled autonomous greenhouse system. The findings revealed profound effect of BL + RL treatment on increased plant height (79.59 cm), stem diameter (10.05 mm), bud length (93.73 mm), and bud diameter (29.87 mm), compared to individual BL and RL treatments and the white light (WL) control. The BL + RL treatment also resulted in shortening the flowering timeline. Maximum flower diameter (18.65 cm) and chlorophyll content index (48.60) were recorded under BL + RL treatment. BL + RL extended post-harvest longevity to 13.2 days, followed by RL (11.6 days). Total chlorophyll content highest at the color bud stage under BL + RL (499.87 μg g⁻¹), though RL treatment sustained the highest levels at the flower stage. Carotenoids content showed a contrasting trend, with RL promoting maximum accumulation during bud stages, while BL + RL enhanced carotenoid synthesis at the flower stage (166.88 μg g⁻¹). Phenol and flavonoid content also reached maximum levels under BL + RL at the color bud stage and anthocyanin content was highest under BL + RL at the flower stage. Additionally, antioxidant activity was significantly higher (86.75 %) in flowers grown under BL + RL. Similarly, up-regulation of flowering genes <em>FT</em> and <em>CO9</em>, particularly observed under the BL + RL treatment at the initial bud and color bud stages, while flowering repressor <em>FLC</em> was down-regulated under all light treatments, facilitating earlier floral induction. The combined BL + RL treatment enhances <em>Lilium</em> cultivation profitability by improving growth, accelerating flowering, increasing flower quality and shelf life, and enabling faster production cycles in controlled Soil-less systems.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"267 ","pages":"Article 113168"},"PeriodicalIF":3.9,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biophysical effects of Phellinus Linteus on hepatocellular carcinoma cells studied by atomic force microscopy","authors":"Yuxi Huang ,&nbsp;Fan Yang ,&nbsp;Chuanzhi Liu ,&nbsp;Guicai Song ,&nbsp;Zuobin Wang","doi":"10.1016/j.jphotobiol.2025.113169","DOIUrl":"10.1016/j.jphotobiol.2025.113169","url":null,"abstract":"<div><div>Precisely quantifying cancer cell morphology and physical properties is crucial for advancing cancer therapeutics, drug screening, and pharmacological assessment. We employed atomic force microscopy (AFM) in this study to accurately measure the morphological and mechanical characteristics of cells and to observe drug-induced cell reactions. To observe the morphological and mechanical properties of both living and fixed HL-7702 cells and SMCC-7721 cells at the nanometer scale. Scanning Electron Microscopy (SEM) analysis revealed that treatment with Phellinus linteus water extract (PLWE) induced a morphological transition in SMCC-7721 cells from an irregular, flattened state to a more rounded and spherical shape, with a significant reduction in membrane structures. These changes are closely associated with apoptotic processes. The morphological changes observed in SMCC-7721 cells, together with MTT assay results, suggest that PLWE possesses substantial antitumor activity against cancer cells while exhibiting low toxicity towards normal cells, highlighting its potential for cell-selective targeting in cancer therapy. This study enhances our understanding of PLWE as an anticancer drug and contributes to novel strategies for precision medicine and personalized individualized treatment.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"267 ","pages":"Article 113169"},"PeriodicalIF":3.9,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143875035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pre-sowing magnetic treatments of seeds improve photosynthetic performance of two photosystems in soybean plants","authors":"Rupal Singh Tomar ,&nbsp;Pinke Patel ,&nbsp;Prabha Rai Kalal ,&nbsp;Pratibha Laad ,&nbsp;K.N. Guruprasad ,&nbsp;Anjana Jajoo","doi":"10.1016/j.jphotobiol.2025.113166","DOIUrl":"10.1016/j.jphotobiol.2025.113166","url":null,"abstract":"<div><div>To investigate the impact of pre-sowing magnetic treatment on soybean plants under natural conditions, a study was carried out focusing on the photosynthetic machinery. Soybean seeds were subjected to magneto-priming through exposure to pulsed magnetic field (PMF, 200 mT for 1 h) and static magnetic field (SMF, 200 mT for 1 h). The dual pulse-amplitude modulator (PAM) technique was employed to assess the simultaneous performance of photosystem II (PSII) and photosystem I (PSI) in magneto-primed soybean seeds. It was observed that after magnetic treatment (MT), the effective quantum yield of photosystem II [Y(II)] exhibited an increase, accompanied by a reduction in non-photochemical quenching (NPQ). Furthermore, the quantum yield of photosystem I [Y(I)] showed enhancement, reaching its maximum in the 200 mT PMF treatment. Non-photochemical quenching was lower in MT-treated seed plants as compared to control, indicating a reduction in energy dissipation and an enhancement in energy transfer from PSII to PSI. The magneto-primed seeds demonstrated an increased electron transport mechanism as evidenced by enhanced ETR(I) and ETR(II). Additionally, lower values of Y(NA) and Y(ND) suggested improved activity of both PSI and PSII in regulating energy transport in cyclic electron flow (CEF) and linear electron flow (LEF). The results suggest that PMF treatment is more beneficial than SMF for enhancing photosynthetic efficiency via regulation of PS II and PSI quantum yield.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"267 ","pages":"Article 113166"},"PeriodicalIF":3.9,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143875034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}