Journal of photochemistry and photobiology. B, Biology最新文献_第4页

Excited-state genistein and its methoxy derivatives with improved antioxidant activity: DFT/TD-DFT calculations, molecular docking, and molecular dynamic simulation 具有提高抗氧化活性的激发态染料木素及其甲氧基衍生物：DFT/TD-DFT计算、分子对接和分子动力学模拟

IF 3.7 2区生物学

Journal of photochemistry and photobiology. B, Biology Pub Date : 2025-08-22 DOI: 10.1016/j.jphotobiol.2025.113245

Xingzhu Tang , Chaofan Sun , Lingling Wang

{"title":"Excited-state genistein and its methoxy derivatives with improved antioxidant activity: DFT/TD-DFT calculations, molecular docking, and molecular dynamic simulation","authors":"Xingzhu Tang , Chaofan Sun , Lingling Wang","doi":"10.1016/j.jphotobiol.2025.113245","DOIUrl":"10.1016/j.jphotobiol.2025.113245","url":null,"abstract":"<div><div>Oxidative stress from excessive accumulation of ROS drives several diseases, highlighting the need for efficient antioxidants. The antioxidant activity of excited-state genistein (Gen) and its methylated derivatives (5-MG, 4′-MG, and 7-MG) is systematically explored using density functional theory/time-dependent density functional theory (DFT/TD-DFT) calculations, molecular docking, and molecular dynamics simulation. Except for 5-MG, the rest of the isoflavones have excited state intramolecular proton transfer (ESIPT) property confirmed by potential energy curves as an ultrafast process, which further determines the molecular forms, namely enol (S<sub>0</sub>) and keto* (S<sub>1</sub>) forms. Especially important, the excited-state isoflavones significantly exhibit increased highest occupied molecular orbital (HOMO) energy value, namely, the enhanced antioxidant activity, with the ESIPT property playing a critical role. Moreover, the introduction of methoxy at 4′ and 7 sites enhances the antioxidant activity, while the phenolic hydroxyl group remains the critical moiety for the antioxidant activity to function as confirmed by atomic dipole moment corrected Hirshfeld population (ADCH). Molecular docking analysis indicates that the binding energy of the excited-state isoflavone to xanthine oxidase (XO) is extremely low, proving that it has a strong inhibitory effect on XO. Furthermore, molecular dynamics simulation demonstrates the stability of the docking model.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"271 ","pages":"Article 113245"},"PeriodicalIF":3.7,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144889263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of drought and leaf age on photosynthetic utilization of fluctuating light in barley 干旱和叶龄对大麦波动光光合利用的影响

IF 3.7 2区生物学

Journal of photochemistry and photobiology. B, Biology Pub Date : 2025-08-21 DOI: 10.1016/j.jphotobiol.2025.113244

Karolina Okoń, Artur Nosalewicz

引用次数: 0

High-resolution imaging of plant delayed luminescence 植物延迟发光的高分辨率成像

IF 3.7 2区生物学

Journal of photochemistry and photobiology. B, Biology Pub Date : 2025-08-21 DOI: 10.1016/j.jphotobiol.2025.113231

Yan-Xia Liu , Hai-Yu Fan , Yu-Hao Wang , Yan-Liang Wang , Sheng-Wen Li , Shi-Jian Li , Xu-Ri Yao , Qing Zhao

{"title":"High-resolution imaging of plant delayed luminescence","authors":"Yan-Xia Liu , Hai-Yu Fan , Yu-Hao Wang , Yan-Liang Wang , Sheng-Wen Li , Shi-Jian Li , Xu-Ri Yao , Qing Zhao","doi":"10.1016/j.jphotobiol.2025.113231","DOIUrl":"10.1016/j.jphotobiol.2025.113231","url":null,"abstract":"<div><div>Delayed luminescence (DL) is a quantized signal that is characteristic of photoexcited molecules entering a relaxed state. Studying DL provides critical insight into photophysical mechanisms through the analysis of specific spatiotemporal dynamics. In this study, we developed a high-sensitivity DL imaging system using a quantitative scientific complementary metal-oxide-semiconductor (qCMOS) camera and a single-photon counting resolution. By optimizing the optical architecture and signal processing algorithms together, we achieved full-field spatiotemporal DL imaging at megapixel resolution (i.e., 2304 × 4096 pixels). Key findings include the following: (1) we observed spatial heterogeneity in DL intensity across the leaves of <em>Arabidopsis thaliana</em>, with stronger signals detected in veins and at sites of mechanical injury; (2) species-specific DL responses occur in response to oxidative stress, with <em>Hydrocotyle vulgaris</em> and <em>Ginkgo biloba</em> showing enhanced central DL activity; (3) excitation using white light induced maximum DL intensity, while red and blue light differentially modulated decay kinetics. Finally, we develop a two-level quantum model that links DL dynamics to the populations of excited-state electrons, thereby developing a theoretical framework for future photophysical research. Collectively, this work establishes a theoretical and technological framework for advancing plant phenotyping under stress conditions and optimizing light environments.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"271 ","pages":"Article 113231"},"PeriodicalIF":3.7,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144904309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of photodynamic reaction by chloroquine through apoptosis induction, lipid peroxidation augmentation, and autophagy inhibition using IR-783 氯喹通过诱导细胞凋亡、增强脂质过氧化和抑制IR-783来增强光动力反应

IF 3.7 2区生物学

Journal of photochemistry and photobiology. B, Biology Pub Date : 2025-08-20 DOI: 10.1016/j.jphotobiol.2025.113242

Natalia Lange , Wojciech Szlasa , Dominika Kunachowicz , Jarosław Kalinin , Julita Kulbacka

{"title":"Enhancement of photodynamic reaction by chloroquine through apoptosis induction, lipid peroxidation augmentation, and autophagy inhibition using IR-783","authors":"Natalia Lange , Wojciech Szlasa , Dominika Kunachowicz , Jarosław Kalinin , Julita Kulbacka","doi":"10.1016/j.jphotobiol.2025.113242","DOIUrl":"10.1016/j.jphotobiol.2025.113242","url":null,"abstract":"<div><div>Photodynamic therapy (PDT) is emerging as a promising approach to enhance cancer treatment by improving therapeutic efficacy, reducing side effects, limiting drug resistance, increasing tumor-specific drug uptake, and preventing metastasis. Second-generation photosensitizers such as IR-783, a cyanine dye, offer high tissue permeability and superior imaging capabilities. This study aimed to evaluate the impact of chloroquine (CQ), an autophagy inhibitor, on the effectiveness of PDT using IR-783 and its potential role in counteracting PDT resistance. Confocal microscopy imaging of the C32 cell line treated with IR-783 revealed a significant increase in caspase-3/−7 activity and enhanced lipid peroxidation following irradiation. CQ exhibited synergistic cytotoxicity when combined with IR-783, reducing cell viability by nearly 50 percentage points. CQ treatment led to increased autophagosome formation and accumulation due to lysosomal impairment, enhancing the sensitivity of cells to IR-783 and highlighting its potential in overcoming PDT resistance.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"271 ","pages":"Article 113242"},"PeriodicalIF":3.7,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144904310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication of doxycycline-loaded mesoporous polydopamine nanoparticles for pelvic infection nursing care via synergistic photodynamic therapy 负载多西环素的介孔聚多巴胺纳米颗粒在盆腔感染护理中的协同光动力治疗

IF 3.7 2区生物学

Journal of photochemistry and photobiology. B, Biology Pub Date : 2025-08-20 DOI: 10.1016/j.jphotobiol.2025.113243

Shuhua Fan , Jing Zhang , Yongna He , Yi Wang , Yanru Yu

{"title":"Fabrication of doxycycline-loaded mesoporous polydopamine nanoparticles for pelvic infection nursing care via synergistic photodynamic therapy","authors":"Shuhua Fan , Jing Zhang , Yongna He , Yi Wang , Yanru Yu","doi":"10.1016/j.jphotobiol.2025.113243","DOIUrl":"10.1016/j.jphotobiol.2025.113243","url":null,"abstract":"<div><div>Pelvic infection has emerged as a significant health risk to women, making the accurate identification of possible pathogenic bacteria and pathogens crucial for its prevention and treatment. Photodynamic therapy (PDT) for antibacterial purposes has emerged as an alternative and promising therapeutic approach for pelvic infections. In this investigation, we developed new antibacterial nanomaterials (DCMP NPs) using doxycycline (DCL), chitosan (CH), and mesoporous polydopamine (MPD) nanoparticles. Further, we evaluated the physicochemical properties of the DCMP NPs. TEM images indicated that fabricated DCMP NPs were spherical, with a particle size of 160.54 ± 4.56 nm. The findings showed that DCMP NPs (150 μg/mL) demonstrated favorable cytocompatibility in NIH3T3 cells and hemocompatibility, exhibiting remarkable antibacterial efficacy against Gram-negative bacteria (<em>E. coli</em>) and Gram-positive bacteria (<em>S. aureus</em>) following blue laser irradiation (1 W/cm<sup>2</sup>, 450 nm). The antibacterial biofilm impact of DCMP NPs was assessed using Live/Dead staining. The laser-irradiated DCMP NPs demonstrated the most pronounced anti-biofilm impact. Overall, DCMP NPs effectively addressed nursing care for bacterial pelvic infections.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113243"},"PeriodicalIF":3.7,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144996982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis and photophysical properties of a chlorin conjugate with a photoswitchable local anesthetic 具有光开关局部麻醉剂的氯偶联物的合成及其光物理性质

IF 3.7 2区生物学

Journal of photochemistry and photobiology. B, Biology Pub Date : 2025-08-08 DOI: 10.1016/j.jphotobiol.2025.113238

Dmitriy A. Minakov , Anton E. Egorov , Alexey N. Noev , Petr V. Ostroverkhov , Nikita V. Suvorov , Maxim N. Usachev , Dmitriy A. Cheshkov , Alexey A. Kostyukov , Tatiana D. Nekipelova , Evgeny A. Zevakin , Vladimir A. Kuzmin , Elena V. Filonenko , Mikhail A. Grin

{"title":"Synthesis and photophysical properties of a chlorin conjugate with a photoswitchable local anesthetic","authors":"Dmitriy A. Minakov , Anton E. Egorov , Alexey N. Noev , Petr V. Ostroverkhov , Nikita V. Suvorov , Maxim N. Usachev , Dmitriy A. Cheshkov , Alexey A. Kostyukov , Tatiana D. Nekipelova , Evgeny A. Zevakin , Vladimir A. Kuzmin , Elena V. Filonenko , Mikhail A. Grin","doi":"10.1016/j.jphotobiol.2025.113238","DOIUrl":"10.1016/j.jphotobiol.2025.113238","url":null,"abstract":"<div><div>A conjugate of natural chlorin and azobenzene bound via a linker was synthesized, its stability and photophysical properties were studied. This conjugate can be proposed as a prototype of a dual-action photopharmacological agent, which acts as a photosensitizer for photodynamic therapy and as a local anesthetic agent with light-controlled activity. Conjugate irradiation by red light (660 nm), results in the triplet state formation (<em>k</em><sub>T</sub> = 2.0 × 10<sup>3</sup> s<sup>−1</sup>) that leads to singlet oxygen generation. Simultaneously endothermic intramolecular triplet-triplet energy transfer (<em>k</em><sub>TET</sub> = 3.3 × 10<sup>5</sup> s<sup>−1</sup>) from the triplet chlorin chromophore to the azobenzene fragment take place, which led to isomerization of the latter. Singlet oxygen reacts with the conjugate linker, which leads to the formation of various cleavage products.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"271 ","pages":"Article 113238"},"PeriodicalIF":3.7,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144860491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Red and blue light differentially regulate carbon metabolism and stem elongation in soybean cultivars with contrasting lodging resistance under shade: A multi-omics perspective 多组学视角下，红蓝光对遮荫下抗倒伏大豆品种碳代谢和茎伸长的调控差异

IF 3.7 2区生物学

Journal of photochemistry and photobiology. B, Biology Pub Date : 2025-08-07 DOI: 10.1016/j.jphotobiol.2025.113241

Chang Wang , Shuo Huang , Xulang Zhang , Fuxin Shan , Jinsheng Fan , Xiaochen Lyu , Shoukun Dong , Chunmei Ma , Baiwen Jiang , Zhenglei Di

{"title":"Red and blue light differentially regulate carbon metabolism and stem elongation in soybean cultivars with contrasting lodging resistance under shade: A multi-omics perspective","authors":"Chang Wang , Shuo Huang , Xulang Zhang , Fuxin Shan , Jinsheng Fan , Xiaochen Lyu , Shoukun Dong , Chunmei Ma , Baiwen Jiang , Zhenglei Di","doi":"10.1016/j.jphotobiol.2025.113241","DOIUrl":"10.1016/j.jphotobiol.2025.113241","url":null,"abstract":"<div><div>This study employed soybean cultivars ‘Henong 60’ (lodging-resistant) and ‘Heinong 48’ (lodging-prone) to investigate the effects of red and blue light on plant height, stem chloroplast ultrastructure, cell wall composition, non-structural carbohydrate (NSC) content, and sugar metabolism-related enzyme activities under 70 % and 100 % shading. Integrated proteomics and metabolomics elucidated carbon allocation pathways during blue light-regulated stem development. Results demonstrated that blue light, compared to red light, significantly inhibited plant height, with stronger inhibition under 100 % shading. Blue light promoted tight stacking of chloroplast grana lamellae, reduced starch granule volume proportion, and redirected carbon allocation from sucrose (−12.42 % to −18.05 %) and starch (−14.55 % to −29.63 %) to cellulose (+3.12 % to +12.47 %) and hemicellulose (+3.67 % to +11.86 %). This shift correlated with blue light-induced upregulation of cellulose synthase, uridine diphosphate glucose (UDP-Glc), and key Calvin cycle enzymes (rbcL, PGK, GAPDH), alongside downregulation of cellulase, sucrose synthase, and cell wall relaxation-related proteins. The lodging-prone cultivar HN48 exhibited greater sensitivity to blue light and higher efficiency in carbon allocation toward cell wall synthesis. This study reveals that blue light enhances stem mechanical strength by remodeling chloroplast carbon metabolism and promoting carbon reallocation from sucrose/starch reserves to cell wall biosynthesis, providing a theoretical basis for optimizing light quality to improve soybean stress resistance.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"271 ","pages":"Article 113241"},"PeriodicalIF":3.7,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144814028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Defining safe operational conditions for two-photon imaging with rhodamine B and SYTO 9 dual-staining 定义罗丹明B和SYTO 9双染色双光子成像的安全操作条件

IF 3.7 2区生物学

Journal of photochemistry and photobiology. B, Biology Pub Date : 2025-07-31 DOI: 10.1016/j.jphotobiol.2025.113230

Yong Seok Lee , Hyun Seok Ryu , Celine Abueva , Andrew Padalhin , So Young Park , Phil-Sang Chung , Seung Hoon Woo

{"title":"Defining safe operational conditions for two-photon imaging with rhodamine B and SYTO 9 dual-staining","authors":"Yong Seok Lee , Hyun Seok Ryu , Celine Abueva , Andrew Padalhin , So Young Park , Phil-Sang Chung , Seung Hoon Woo","doi":"10.1016/j.jphotobiol.2025.113230","DOIUrl":"10.1016/j.jphotobiol.2025.113230","url":null,"abstract":"<div><div>Two-photon microscopy (TPM) frequently employs dual-staining techniques for simultaneous visualization of cellular structure and viability. Despite widespread use, the safety of combining Rhodamine B (RB) and SYTO 9 dyes under 920 nm excitation has not been thoroughly assessed. This study systematically evaluated cytotoxicity, sensitization, and dermal irritation of RB (20 μg/mL) and SYTO 9 (2.7 μg/mL) under typical TPM conditions. BJ fibroblasts and THP-1 monocytic cells were exposed to dye-laser treatments (6–60 mW/cm<sup>2</sup> for 10 min), followed by CCK-8 viability assays and sensitization evaluation via modified human Cell Line Activation Test (h-CLAT). For dermal irritation assessment, BALB/c mice underwent topical application of dyes and laser exposure, followed by histological and macroscopic evaluations. Results showed maintenance of cell viability (>70 %) across all laser intensities, with no indication of sensitization, as evidenced by low CD54 and CD86 expression, and negligible skin irritation (Primary Irritation Index ≤0.5). Histological analysis confirmed no significant inflammatory or structural damage. These comprehensive findings establish an ISO-compliant safety threshold, confirming that RB and SYTO 9 dual-staining under 920 nm TPM conditions pose no significant biological risks.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"271 ","pages":"Article 113230"},"PeriodicalIF":3.7,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144779285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential UVC radiation sensitivity in multidrug-resistant l1210 cells: Insights into p53 and Bcl-XL expression/function 多药耐药l1210细胞UVC辐射敏感性的差异：p53和Bcl-XL表达/功能的见解

IF 3.7 2区生物学

Journal of photochemistry and photobiology. B, Biology Pub Date : 2025-07-30 DOI: 10.1016/j.jphotobiol.2025.113229

Lucia Pavlikova , Jakub Krepop , Zdena Sulova , Albert Breier , Mario Seres

{"title":"Differential UVC radiation sensitivity in multidrug-resistant l1210 cells: Insights into p53 and Bcl-XL expression/function","authors":"Lucia Pavlikova , Jakub Krepop , Zdena Sulova , Albert Breier , Mario Seres","doi":"10.1016/j.jphotobiol.2025.113229","DOIUrl":"10.1016/j.jphotobiol.2025.113229","url":null,"abstract":"<div><div>Multidrug-resistant (MDR) variants of L1210 cells, selected for resistance to vincristine (R) or doxorubicin (D), exhibit elevated ABCB1 (P-glycoprotein) expression but differ in UVC sensitivity. D cells resemble parental L1210 (S) cells, whereas R cells are significantly more UVC vulnerable. To investigate this, we analyzed the expression of genes involved in DNA damage response, Trp53 family members, cyclin-dependent kinase inhibitors (p15, p21), Bcl-2 family genes, and DNA repair genes in S, R, and D cells before and after UVC irradiation.</div><div>The most striking difference was the absence of <em>Trp53</em> expression in R cells at both mRNA and protein levels, while S and D cells expressed this gene. Instead, R cells uniquely expressed <em>Trp63</em>. CRISPR/Cas9-generated p53-null mutants of S and D cells showed increased UVC-induced cell death, but their sensitivity did not reach that of R cells.</div><div>Bcl-XL, a protein linked to resistance against UVC-induced apoptosis, was also reduced at both transcript and protein levels in R cells. Pharmacological inhibition of Bcl-XL in S and D cells with A-1155463 and A-1331852 enhanced UVC-induced cell death but did not replicate the high sensitivity observed in R cells.</div><div>Our findings suggest that the heightened UVC sensitivity of R cells results from a combined deficiency of p53 and Bcl-XL, impairing DNA damage response and apoptosis. These results reveal distinct molecular adaptations in MDR variants and provide insight into the mechanisms underlying differential UVC sensitivity.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"271 ","pages":"Article 113229"},"PeriodicalIF":3.7,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144768727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental study on photodynamic therapy of CCA assisted by electrolytic oxygen supply based on black phosphorus nanocomposites 基于黑磷纳米复合材料的电解供氧辅助CCA光动力治疗实验研究。

IF 3.7 2区生物学

Journal of photochemistry and photobiology. B, Biology Pub Date : 2025-07-29 DOI: 10.1016/j.jphotobiol.2025.113228

Xinqiang Wu , Xiaofeng Jiang , Zhaoyuan Liu , Yuanfeng Gong , Ping Xue , Yunqiang Tang

{"title":"Experimental study on photodynamic therapy of CCA assisted by electrolytic oxygen supply based on black phosphorus nanocomposites","authors":"Xinqiang Wu , Xiaofeng Jiang , Zhaoyuan Liu , Yuanfeng Gong , Ping Xue , Yunqiang Tang","doi":"10.1016/j.jphotobiol.2025.113228","DOIUrl":"10.1016/j.jphotobiol.2025.113228","url":null,"abstract":"<div><div>Cholangiocarcinoma (CCA) is the second most common malignant liver tumor after hepatocellular carcinoma. It is highly aggressive, and most patients are diagnosed at an advanced stage, losing the opportunity for surgical resection. What's worse, the palliative radiotherapy and chemotherapy have limited efficacy. Photodynamic therapy (PDT), based on photosensitizers, is a new treatment modality following surgery and chemoradiotherapy for tumors. Clinically, hematoporphyrin derivative preparations are commonly chosen as photosensitizers for PDT in CCA. Although these derivatives are widely used and their efficacy has been validated by numerous clinical studies, the actual long-term outcome mostly remains unsatisfactory. This is primarily due to their low efficiency in generating reactive oxygen species (ROS) and the inherently hypoxic microenvironment of CCA, which limits their function. To address these concerns, we constructed a nanocomposite based on black phosphorus nanosheets loaded with Fe<sub>3</sub>O<sub>4</sub> nanoparticles, assisted by electrolytic oxygen supply. The study aims to enhance the cytotoxicity against CCA by leveraging the efficient singlet oxygen generation of black phosphorus and the Fe<sub>3</sub>O<sub>4</sub>-catalyzed hydroxyl radical production from hydrogen peroxide. Additionally, it proposes to improve the hypoxic microenvironment of CCA and increase the tumor's sensitivity to PDT by using direct current electrolysis (DCE) to generate oxygen. The primary focus of this research is to explore the regulatory mechanisms of hypoxia-PDT for CCA based on ultrathin black phosphorus nanosheets, providing new insights for optimizing clinical PDT for CCA.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"271 ","pages":"Article 113228"},"PeriodicalIF":3.7,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144768728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0