Journal of photochemistry and photobiology. B, Biology最新文献

{"title":"The synergistic effect of pulsed red light and leonurus inhibits primary dysmenorrhea induced by oxytocin in mice by modulating calcium signaling and inhibiting inflammatory responses","authors":"Qiqi Fu ,&nbsp;Hui Jiang ,&nbsp;Jiali Yang ,&nbsp;Haokuan Qin ,&nbsp;Longfei Huo ,&nbsp;Yi Ren ,&nbsp;Shangfei Lin ,&nbsp;Muqing Liu ,&nbsp;Jinghui Yao","doi":"10.1016/j.jphotobiol.2024.113079","DOIUrl":"10.1016/j.jphotobiol.2024.113079","url":null,"abstract":"<div><div>Despite increasing evidence suggesting that red light photobiomodulation (R-PBM) and leonurus play important roles in analgesic and anti-inflammatory processes, data on their combined effect on primary dysmenorrhea (PD) are scarce. In this study, we reported the pain assessment of red light at various modes combined with leonurus on the oxytocin-induced model of PD mice. The combined intervention of pulsed R-PBM and leonurus decreased pain responses and PGF_2α/PGE₂ levels, alleviated uterine swelling and inflammatory infiltration, enhanced antioxidant levels (T-AOC, GSH-PX, SOD), and reduced lipid peroxidation (MDA, LPO) in the uterus, with its synergistic effect surpassing either treatment alone or the combination of continuous wave R-PBM with leonurus. Transcriptomic analysis demonstrated significant changes in differentially expressed genes associated with calcium signaling (<em>Cav1, Cacna1c, Kcnmb1, Cnn1,</em> and <em>Myh11</em>) and inflammatory response (<em>Ptgs2, Jun, Fos, IL1rn,</em> and <em>IL17b</em>) in the combination group, with concurrent downregulation of MLCK, COX-2, p-JNK/JNK, and IL17b protein levels, and upregulation of IL1rn, suggesting that the combined intervention of pulsed R-PBM and leonurus may alleviate pain through disruption of calcium homeostasis and induction of ROS-mediated inflammatory responses. Metabolomics studies of plasma revealed significant changes in lipid metabolism after the combined intervention, consistent with the transcriptomic findings. Hence, pulsed R-PBM combined leonurus has the potential to be an effective therapeutic approach for PD, as well as an alternative option for painful and inflammatory diseases; however, further exploration of its underlying mechanism is still necessary.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"262 ","pages":"Article 113079"},"PeriodicalIF":3.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142812952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of daytime light intensity on daily behaviours and concurrent hypothalamic gene expressions in migratory redheaded bunting","authors":"Jyoti Tiwari ,&nbsp;Sayantan Sur ,&nbsp;Asma Naseem ,&nbsp;Preeti Gupta ,&nbsp;Shirin Fatima ,&nbsp;Sangeeta Rani ,&nbsp;Shalie Malik","doi":"10.1016/j.jphotobiol.2024.113081","DOIUrl":"10.1016/j.jphotobiol.2024.113081","url":null,"abstract":"<div><div>Animals use photic cues to time their daily and seasonal activity. The role of photoperiod has been much investigated in seasonal responses, but the role of light intensity is less understood in passerine finches. We investigated if and how daytime light intensity influences photoinduced migratory phenologies and hypothalamic mRNA expressions in a Palearctic-Indian migratory finch, redheaded bunting (<em>Emberiza bruniceps</em>). Photoperiodic manipulations were employed to induce winter-nonmigratory (NM), premigratory (PM), and migratory (MIG) states in photosensitive buntings. In each life history state, the birds were further subjected to 0.055 (low), 0.277 (medium), or 1.11 W/m² (high) (<em>N</em> = 5 each) light intensity treatment. The low daytime light intensity dampened the locomotor activity rhythm and delayed the onset of <em>Zugunruhe</em>. We found life history-dependant but not light intensity-dependant changes in body mass, fat score, and testis volume. Plasma corticosterone levels were increased under the low-light intensity group in the migratory state. The buntings were foraging throughout the night in the migratory state, aiding body fattening. Front and back sleep were drastically reduced during the migratory phase under all three light intensities. In the migratory state, we found elevated hypothalamic <em>IL1B</em> and <em>IL6</em> expression in medium and high-light intensity groups, which had significantly reduced sleep duration. In the winter nonmigratory state, the expression of <em>CAMK2</em> correlated with daytime activity and active wakefulness of buntings. The decreased <em>GHRH</em> expression correlates with the reduction in total sleep in migrating buntings. Overall, daytime light intensity emerges as a key factor that fine-tunes the photoperiodic response and regulates active and sleep behaviour in migratory buntings.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"262 ","pages":"Article 113081"},"PeriodicalIF":3.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142846918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photoprotection and antioxidant activity of eumelanin from Streptomyces lasalocidi NTB 42 and its photoprotective effects on Schizosaccharomyces pombe ARC039","authors":"Muhammad Asril ,&nbsp;Rika Indri Astuti ,&nbsp;Iman Rusmana ,&nbsp;Aris Tri Wahyudi","doi":"10.1016/j.jphotobiol.2024.113085","DOIUrl":"10.1016/j.jphotobiol.2024.113085","url":null,"abstract":"<div><div>This study evaluated the photoprotective and antioxidant properties of eumelanin derived from <em>Streptomyces lasalocidi</em> NTB 42 (eumelanin NTB 42). This study also investigated the cellular-level photoprotective effects of eumelanin using <em>Schizosaccharomyces pombe</em> ARC039 as a model organism and its ability to enhance the Sun Protection Factor (SPF) of commercial sunscreens. The thermal and light stability and total phenolic and flavonoid contents were analyzed. Antioxidant activity was assessed using hydroxyl radicals (OH<img>), and superoxide anions (O₂^.-) radical-scavenging assays. The efficacy of photoprotection was determined using various in vitro methods, yeast cell viability assays, and enhanced SPF values of commercial sunscreen products. Eumelanin NTB 42 exhibited both thermal and photostabilities. The total phenolic and flavonoid contents were reported as 88.82 ± 0.68 mg gallic acid equivalent (GAE)/g and 53.24 ± 2.66 mg quercetin equivalent (QE)/g, respectively, representing the first report on microbial eumelanin. Eumelanin NTB 42 demonstrated significant scavenging activity against OH<img> and O₂^.-. It also displays notable photoprotective effects against UV-B radiation, offering broad-spectrum coverage and optimal protection against UV-A radiation. It effectively acted as a sunblock against UV-A and UV-B radiation. Furthermore, eumelanin NTB 42 enhanced <em>S. pombe</em> ARC039 cell viability after exposure to UV-B and UV-C for 30–90 min. It also augmented the SPF value of commercial products at a minimum concentration of 0.0025 %. These findings highlight the potential antioxidant and photoprotective properties of eumelanin NTB 42, suggesting its applicability as a raw material for sunscreen formulations in the cosmetic industry.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"262 ","pages":"Article 113085"},"PeriodicalIF":3.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the antineoplastic properties of the photosensitizer biscyanine in 2D and 3D tumor cell models and artificial skin models","authors":"Pedro Victor Silva Resende ,&nbsp;Izabela Natália Faria Gomes ,&nbsp;Maria Clara Peixoto ,&nbsp;Giulia Rodrigues Stringhetta ,&nbsp;Lidia Maria Rebolho Batista Arantes ,&nbsp;Vladimir Alexandrovich Kuzmin ,&nbsp;Iouri Borissevitch ,&nbsp;Rui Manuel Reis ,&nbsp;Vinícius de Lima Vazquez ,&nbsp;Lucimara Perpetua Ferreira ,&nbsp;Renato José Silva Oliveira","doi":"10.1016/j.jphotobiol.2024.113078","DOIUrl":"10.1016/j.jphotobiol.2024.113078","url":null,"abstract":"<div><h3>Background</h3><div>Photodynamic Therapy (PDT) is a therapeutic modality that combines the application of a photoactive compound (photosensitizer, PS) with low-power light to generate reactive oxygen species in the target tissue, resulting in cytotoxic damage and cell death, while sparing adjacent tissues. The objective of this study was to evaluate the phototoxicity of a cyanine dye with two chromophores (biscyanines, BCD) in systems with varying levels of cellular organization, and we used the Photogem® (a photosensitizer approved by the Brazilian ANVISA agency for clinical use in Photodynamic Therapy) as a positive control.</div></div><div><h3>Materials and methods</h3><div>The cytotoxicity of the compounds was assessed <em>in vitro</em> in 2D monolayers, 3D spheroid cultures, and artificial skin models. Four tumoral cell lines A375 (melanoma), HCB-541 (cutaneous squamous cell carcinoma), Vu120T and Vu147T (head and neck cancer), and two normal cell lines fibroblastic HFF-1 and keratinocyte HACAT were used in this study. Cell viability, migration, production of reactive oxygen species, expression of proteins linked to DNA damage and repair, internalization, and skin permeation of PS agents.</div></div><div><h3>Results</h3><div>Light irradiation in the presence of the PS resulted in greater cytotoxic effects for BCD as compared to Photogem®, which was accompanied by an increase in the production of reactive oxygen species including H₂O₂, elevated levels of cleaved PARP, and a higher rate of phosphorylated H2AX protein. BCD demonstrated enhanced internalization and bioaccumulation in the spheroids and equivalent skin models.</div></div><div><h3>Conclusion</h3><div>BCD, as a photosensitizer, showed higher cytotoxicity, with an increased ability to generate reactive oxygen species. This led to reduced cell viability, inhibited migration, and upregulated DNA damage-related proteins. Additionally, its enhanced cellular uptake improved skin barrier permeability, making BCD a strong candidate for <em>in vivo</em> Photodynamic Therapy.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"262 ","pages":"Article 113078"},"PeriodicalIF":3.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Near-infrared light therapy normalizes amyloid load, neuronal lipid membrane order, rafts and cholesterol level in Alzheimer's disease","authors":"Iuliia Golovynska ,&nbsp;Sergii Golovynskyi ,&nbsp;Yurii V. Stepanov ,&nbsp;Jinghan Qu ,&nbsp;Renlong Zhang ,&nbsp;Junle Qu","doi":"10.1016/j.jphotobiol.2024.113086","DOIUrl":"10.1016/j.jphotobiol.2024.113086","url":null,"abstract":"<div><div>Cholesterol dysregulation, disorder of neuronal membrane lipid packing, and lipid rafts lead to the synthesis and accumulation of toxic amyloid-β (Aβ), contributing to the development of Alzheimer's disease (AD). Our study shows that near-infrared (NIR) transcranial photobiomodulation therapy (tPBMT) can reduce Aβ load and restore the properties of neuronal plasma membrane, including Aβ production, bilayer order, rafts, lipid content, and Ca²⁺ channels during AD. Mice in the experiments were exposed to 808-nm LED for 1 h daily over 3 months. In the APOE transgenic model with cholesterol dysregulation, the cholesterol levels increased by 22 times, causing healthy neurons to produce toxic Aβ three times faster, increasing its load by five times. Consequently, Aβ disrupts the membrane bilayer and prompts the formation of lipid rafts and pores. NIR-tPBMT can nearly half attenuate Aβ load, restore membrane lipid order and rigidity, reduce the number of lipid rafts, modulate cholesterol synthesis, normalize Ca²⁺ influx by activated endocytosis, and reduce neuronal death. The Ca²⁺ influx induced by light does not cause excitotoxicity but modulates Ca²⁺/calmodulin signaling involved in AD mechanisms and cell viability. The transcriptome analysis of the brain cortex and hippocampus shows that light can downregulate Ca²⁺/calmodulin-dependent AD-risk genes BACE, PSEN, and APP, and normalize cholesterol homeostasis via the HMGCR, DHCR7, and INSIG1 genes. Additionally, light enhances neuron resistance to the endoplasmic reticulum stress via activating transcription factors of the unfolded protein response. Thus, red/NIR light could be promising in combating AD, restoring synaptic plasticity in degenerating neurons and reducing Aβ load.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"262 ","pages":"Article 113086"},"PeriodicalIF":3.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel peptide inhibitor of matrix Metalloproteinases-1 from pufferfish skin collagen hydrolysates and its potential Photoprotective activity via the MAPK/AP-1 signaling pathway","authors":"Bei Chen ,&nbsp;Shuilin Cai ,&nbsp;Lulu Cui ,&nbsp;Ting Yu ,&nbsp;Kun Qiao ,&nbsp;Yongchang Su ,&nbsp;Min Xu ,&nbsp;Haiyan Tang ,&nbsp;Shuji Liu ,&nbsp;Ming Yang ,&nbsp;Zhiyu Liu","doi":"10.1016/j.jphotobiol.2024.113088","DOIUrl":"10.1016/j.jphotobiol.2024.113088","url":null,"abstract":"<div><div><em>Takifugu bimaculatus,</em> a pufferfish species farmed in Fujian Province, is known for its non-toxic flesh and collagen-rich skin. We identified a novel collagen-derived matrix metalloproteinase 1 (MMP-1) inhibitory peptide, from <em>T. bimaculatus</em> skin with potent anti-photoaging properties. Using multistage membrane and gel filtration chromatography, we purified low-molecular-weight collagen peptides from <em>T. bimaculatus</em> skin (TBSCH-L). Nano-HPLC-MS/MS and virtual molecular docking screening were employed to identify peptides targeting MMP-1. Four anti-photoaging peptide sequences, GDRGFPGE, GPAGPRGA, FPGGPGAK, and RGFPGGDGAA, were identified by assessing the viability of UVB-induced L929 cells. GPAGPRGA (GP8) exhibited the highest MMP-1 inhibitory activity and cellular photoprotection. Surface plasmon resonance confirmed high-affinity binding between MMP-1 and GP8. GP8 significantly reduced intracellular reactive oxygen species (ROS) levels and enhanced superoxide dismutase activity at concentrations of 100–200 μM in UVB-exposed L929 cells. At 200 μM, GP8 significantly decreased malondialdehyde content. GP8 also accelerated migration of L929 cells, demonstrating its wound-healing potential, markedly reduced intracellular β-galactosidase levels, and downregulated phosphorylation levels of extracellular signal-regulated kinases, c-Jun N-terminal kinases, p38 proteins, and c-Jun protein expression within the MAPK/AP-1 signaling pathway, thereby lowering MMP expression in L929 cells. Exposure of zebrafish to 25–100 μM GP8 effectively mitigated UVB-induced damage, restoring up to 31.2 % of caudal fin integrity, while significantly reducing ROS levels, lipid peroxidation, and cellular apoptosis. GP8, a novel marine-derived anti-photoaging peptide, holds promise for applications in cosmetic and functional food sectors.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"262 ","pages":"Article 113088"},"PeriodicalIF":3.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deep dive into the diversity and properties of rhodopsins in actinomycetes of the family Geodermatophilaceae","authors":"Sergey V. Tarlachkov ,&nbsp;Irina P. Starodumova ,&nbsp;Olga V. Boueva ,&nbsp;Sergei V. Chernyshov ,&nbsp;Lyudmila I. Evtushenko","doi":"10.1016/j.jphotobiol.2024.113083","DOIUrl":"10.1016/j.jphotobiol.2024.113083","url":null,"abstract":"<div><div>In recent decades, most studies of microbial rhodopsins have focused on their identification and characterization in aquatic bacteria. In 2021, actinomycetes of the family <em>Geodermatophilaceae</em>, commonly inhabiting terrestrial ecosystems in hot and arid regions, have been reported to contain rhodopsins with DTEW, DTEF and NDQ amino acid motifs. An advanced bioinformatics analysis performed in this work additionally revealed NTQ rhodopsin and heliorhodopsins. The absorption maxima identified for rhodopsins from the above five groups ranged from 513 nm (NTQ rhodopsin) to 559 nm (heliorhodopsin). An assessment of pumping specificity showed that DTEW and DTEF rhodopsins possessed outward H⁺-transport activities. Ca²⁺ ions were required for pumping if <em>E. coli</em> C43(DE3) was used as an expression strain, but were unnecessary in the case of <em>E. coli</em> BL21(DE3). For NDQ rhodopsin, outward H⁺-transport was detected in NaCl and KCl solutions at pH 5 and 6, but not at neutral pH. A weak Na⁺-efflux was observed for this rhodopsin at pH 6 and 7 in a NaCl solution only in the presence of proton ionophore. NTQ rhodopsin acted as an inward Cl⁻-, Br⁻-, and I⁻- pump, with a much weaker activity towards NO³⁻. No pumping activity was detected for the heliorhodopsin tested. The finding of rhodopsins with novel properties further expands the rhodopsin landscape.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"262 ","pages":"Article 113083"},"PeriodicalIF":3.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Excited-state antioxidant activity for apigenin based on external electric field-modulated ESIPT behavior: TD-DFT and molecular docking calculations","authors":"Xingzhu Tang ,&nbsp;Ye Wang ,&nbsp;Lingling Wang ,&nbsp;Yajie Zhang ,&nbsp;Chaofan Sun","doi":"10.1016/j.jphotobiol.2024.113087","DOIUrl":"10.1016/j.jphotobiol.2024.113087","url":null,"abstract":"<div><div>Apigenin (Api), a flavonoid possessing dual features of antioxidant activity and intramolecular hydrogen bond (IMHB), is subjected to an external electric field (EEF) to investigate its excited-state antioxidant activity after excited state intramolecular proton transfer (ESIPT) behavior employing the density functional theory (DFT) and time-dependent DFT (TD-DFT) methods, as well as molecular docking. The existence of IMHB is demonstrated by structural parameters and AIM topological analysis, where Api in the enol^⁎ form under an EEF of +60 × 10⁻⁴ a.u. possesses strong IMHB. The potential energy curves confirm that the ESIPT process varies from barrierless to barriered as the positive EEF grows, thus determining the excited-state form. Api exhibits strong excited-state antioxidant activity in vitro whether or not under an EEF, especially under the EEF of −40 × 10⁻⁴ a.u., utilizing HOMO energy. According to average local ionization energy (ALIE), the electrophilic reaction site also changes after ESIPT process under the EEF, and the activity is significantly increased. Furthermore, activation of the antioxidant Keap1-Nrf2-ARE pathway in vivo, namely, the interaction of Keap1 protein with Api, calculated by molecular docking, suggests that an interaction between the Keap1 and excited-state Api exists accompanying lower and variable bind energy under the distinct EEFs. Taken together, combining the modulation of the ESIPT process with the excited-state antioxidant activity is an effective approach to enhance the antioxidant activity of compounds.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"262 ","pages":"Article 113087"},"PeriodicalIF":3.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of photobiomodulation on human hair dermal papilla cells with various light modes and light parameters","authors":"Yi Ren ,&nbsp;Angze Li ,&nbsp;Xiaojing Miao ,&nbsp;Longfei Huo ,&nbsp;Haokuan Qin ,&nbsp;Hui Jiang ,&nbsp;Muqing Liu","doi":"10.1016/j.jphotobiol.2024.113080","DOIUrl":"10.1016/j.jphotobiol.2024.113080","url":null,"abstract":"<div><div>Androgenetic alopecia (AGA) is a prevalent hair loss disorder and influenced by genetic, hormonal, and environmental factors. Minoxidil and finasteride have been widely used for treating AGA. However, the side effects associated with these drugs often lead to poor patient compliance. In contrast, photobiomodulation (PBM), due to its safety and non-invasiveness, holds promising prospects for use. Although the promoting effects of PBM on AGA have been reported, the mechanisms by which PBM affects dermal papilla cells (DPCs) remain largely unknown. Hence, this study explored the impacts of both continuous wave (CW) and pulsed wave (PW) PBM on DPCs, and revealed the underlying actions of light parameters in PBM. Orthogonal experiments were conducted to evaluate the effects of CW PBM on DPCs at varying irradiances and doses, indicating that irradiance was the crucial parameter, as well as cell viability and proliferation were maximized at 8 mW/cm² and 8 J/cm². For PW PBM, response surface methodology was employed to determine the influences of duty cycles, frequencies, and doses. The findings highlighted frequency as a primary factor, with the optimum cell viability observed at peak irradiance 10 mW/cm², duty cycle 80 %, 500 Hz, and 8.8 J/cm². Notably, PBM could enhance cell viability, proliferation, and migration in DPCs by activating the Wnt/β-Catenin signaling and suppressing Transforming Growth Factor signaling, particularly when applied in pulsed mode. Overall, this study determined the key light parameters that influence PBM effectiveness, further identified the optimal light conditions, and preliminary revealed into the mechanisms of PBM in DPCs, highlighting that PW PBM may be a competitive therapeutic option for alleviating AGA in the future.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"262 ","pages":"Article 113080"},"PeriodicalIF":3.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liquid phase preparation and characterization of MgO nanoparticles and their butchery activities against dental bacterial pathogens and human cervical cancer cell line","authors":"Thamizharasan Sambandam ,&nbsp;Gurunathan Karuppasamy ,&nbsp;Govindhan Perumal ,&nbsp;Edison Chandraseelan Rajasingh","doi":"10.1016/j.jphotobiol.2024.113084","DOIUrl":"10.1016/j.jphotobiol.2024.113084","url":null,"abstract":"<div><div>A novel method for synthesizing nanomaterials involves microbial or phytochemical nano-factories, which offer an eco-friendly, cost-effective, and reliable approach to producing clean and reproducible products. In this study, magnesium oxide nanoparticles (MgO NPs) were synthesized using <em>Avicennia marina</em>, a marine plant, as both a nucleation and stabilizing agent. The MgO NPs were characterized for crystallinity, cut-off wavelength, morphology, thermal stability, and surface properties using XRD, EDX, BET, UV–Visible spectroscopy, DLS, zeta potential analysis, SEM, TEM, TGA/DTA, and PL spectroscopy. Using the Brunauer-Emmett-Teller (BET) method, the specific surface area of the MgO nanoparticles was determined to be 97.248 m²/g. The optical band gap energy of the MgO nanoparticles that corresponded to the highest absorption peak was determined to be 5.8 eV. Additionally, the antimicrobial, scavenging, and antioxidant activities of the MgO NPs were evaluated against dental bacterial pathogens. To explore anticancer potential, the MgO NPs were tested on a cervical cancer cell line (HeLa) using an MTT assay, which revealed that cytotoxicity increased with higher MgO NP concentrations. MgO nanoparticles were applied at concentrations of 25 μg/mL, 50 μg/mL, 75 μg/mL, and 100 μg/mL to inhibit the proliferation of cancer cell lines. The cytotoxicity observed at an IC50 concentration of 56.54 μg/mL is primarily influenced by factors such as the small size, increased surface-to-volume ratio, oxygen vacancies, and the morphology of the nanoparticles. This interdisciplinary study contributes to the understanding of MgO NPs and their multifunctional potential in combating dental infections and cervical cancer.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"262 ","pages":"Article 113084"},"PeriodicalIF":3.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}