Journal of photochemistry and photobiology. B, Biology最新文献

{"title":"Transcriptomic profiling of eyeless Drosophila reveals molecular network orchestrating response to blue light","authors":"Jun Yang ,&nbsp;Song Yujuan ,&nbsp;Rujul Kumar ,&nbsp;Subisha Sundaram ,&nbsp;Benjamin Ramsell ,&nbsp;Yanming Di ,&nbsp;Jadwiga Giebultowicz ,&nbsp;David A. Hendrix","doi":"10.1016/j.jphotobiol.2025.113225","DOIUrl":"10.1016/j.jphotobiol.2025.113225","url":null,"abstract":"<div><div>In a modern society where artificial light sources rich in <strong>blue light (BL)</strong> are pervasive, concerns about the potential health impacts of BL on humans are growing. The damage BL causes to the human retina is well established, but its effects on non-retinal cells and the underlying mechanisms remain unclear. This study investigates the effects of phototoxic levels of BL on gene expression in adult <em>Drosophila</em>. We exposed <em>Drosophila</em> with genetically ablated eyes to continuous BL around the clock and assessed transcriptomic changes in non-retinal head tissues using RNAseq at three time points: 6, 10, and 14 days. Transcriptomic data revealed significant number of differentially expressed genes (DEGs) related to ribosomal biogenesis and energy metabolism pathways, including G6P metabolic process, fatty acyl-CoA biosynthesis, and glycolysis/ gluconeogenesis, due to BL exposure. To identify potential <strong>transcription factors (TFs)</strong> that may be involved in the BL-induced changes in gene expression, we utilized database of motifs to search the promoters and genomic sequences including introns of genes identified as DEGs. We determined that promoters for upregulated DEGs contain a DNA motif that is predicted to be bound by a TF with homology to a plant TF which is activated by light, encoded by <em>CoRest</em>. Further, a significant number of DEGs contained DNA motifs that could be targeted by another TF encoded by <em>Xrp1</em>, whose expression is significantly upregulated in BL. To test functional involvement of <em>Xrp1</em> in BL response, we induced its overexpression in <em>Drosophila</em> neurons and showed that this significantly increased fly survival under BL, while knock out of <em>Xrp1</em> accelerated mortality of BL-exposed flies. This study implicates potential positive feedback loop between <em>CoRest</em> and <em>Xrp1</em>, which is supported by previously published ChIPseq data, in response to blue light exposure and provides novel insights into the molecular mechanisms involved in mitigating its adverse effects.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"271 ","pages":"Article 113225"},"PeriodicalIF":3.7,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144768729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-functional fluorescence probe for revealing metabolism during cellular cuproptosis","authors":"Junyan Ma ,&nbsp;Mingtao Zhao ,&nbsp;Dan Meng ,&nbsp;Zilin Jiao ,&nbsp;Weijie Si ,&nbsp;Zhenxing Zhang","doi":"10.1016/j.jphotobiol.2025.113226","DOIUrl":"10.1016/j.jphotobiol.2025.113226","url":null,"abstract":"<div><div>Cuproptosis, a newly identified type of cell death induced by copper, is associated with intracellular oxidative stress, mitochondrial dysfunction and protein aggregation. This work presents the development of a red-emitting fluorescent probe, <strong>COS-NS</strong> which is applied to reveal the possible metabolism during cellular cuproptosis. This probe facilitates the concurrent and accurate detection of HOCl and viscosity through distinct emission wavelengths, thereby eliminating possible interference. Furthermore, probe <strong>COS-NS</strong> has exhibited characteristics like good selectivity, ultrafast response time (∼ 15 s), low detection limit (81 nM), and exceptional stability, rendering it a suitable instrument for detecting cellular HOCl in biological systems. Moreover, we have indicated that cuproptosis could rise oxidative stress and GSH is a key factor in promoting the cuproptosis with sufficient copper ion (II) within biosystem.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"270 ","pages":"Article 113226"},"PeriodicalIF":3.9,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144680576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A photoinduced nuclear translocation of thiophene derivative for selective chemotherapy","authors":"Dat Thanh Dinh ,&nbsp;Yi-Liang Liou ,&nbsp;Tzong-Shiun Li ,&nbsp;Cheng-Chung Chang","doi":"10.1016/j.jphotobiol.2025.113223","DOIUrl":"10.1016/j.jphotobiol.2025.113223","url":null,"abstract":"<div><div>In this study, a novel thiophene-based small organic molecule, 2,5-bis(2-(N-butylpyridinium-4-yl)vinyl)-thiophene diiodine (BBT), was prepared. BBT not only presented selective dark/photo dual cell toxicity to cancer cells but also acted as a nuclear transport inducer for the chemotherapy agent doxorubicin (DXR), thereby promoting anticancer efficacy. BBT exhibits the special aggregation-induced emission (AIE) effect and specifically accumulates in the mitochondria of cancer cells, leading to higher dark toxicity in cancer cells than in normal cells. Interestingly, BBT molecules translocate into the nuclei of cancer cells upon photosensitisation and further selectively exert their phototoxicity on cancer cells. Thanks to the unique characteristics of BBT, a synergistic therapy was optimised based on several combinational treatment pathways between BBT and DXR. The effectiveness of the combinational treatment pathway was evaluated based on the theoretical and actual cell viability rates, the accumulation of DXR in cell nuclei, and the required time interval for the fragmentation of nucleoli. BBT pre-incubation, followed by photosensitisation and then DXR incubation, proved to be the most effective combinational treatment pathway, constituting the synergistic therapy. As a result, BBT is considered a nuclear translocation molecule that can be beneficial for nucleus-targetable chemotherapy drugs to promote nuclear aggregation and drug efficacy. Furthermore, because the nuclear translocation behaviour of BBT is selective in cancer cells, the nuclear aggregation enhancement of chemotherapy drugs can be achieved selectively. In conclusion, we developed a novel synergistic therapy for cancer treatment, and BBT is a potential chemotherapy auxiliary for DXR.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"270 ","pages":"Article 113223"},"PeriodicalIF":3.9,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144662414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural-synthetic hybrid nanoparticles: Polydopamine-Phycocyanin composites for enhanced antibacterial phototherapy against bacterial pathogens","authors":"Armin Fakouri,&nbsp;Matin Mahmoudifard,&nbsp;Nahid Ghorbani,&nbsp;Melika Shafiei,&nbsp;Kambiz Akbari Noghabi","doi":"10.1016/j.jphotobiol.2025.113222","DOIUrl":"10.1016/j.jphotobiol.2025.113222","url":null,"abstract":"<div><div>The rapid rise of antibiotic-resistant bacterial infections demands innovative, non-antibiotic strategies to combat pathogens without driving further resistance. In this study, we developed polydopamine-coated phycocyanin nanoparticles (PDA@PC) by encapsulating the natural antioxidant phycocyanin (PC) within a polydopamine (PDA) matrix. These nanoparticles (146 nm) combine PDA's photothermal and photodynamic properties with PC's antioxidant activity, enabling dual-mode antibacterial action under near-infrared (NIR) laser irradiation. PDA@PC demonstrated excellent biocompatibility (&gt;70 % cell viability) and blood compatibility (&lt;5 % hemolysis), outperforming PDA alone at high doses. Under laser exposure, PDA@PC generated heat and reactive oxygen species (ROS), achieving &gt;50 % inhibition of bacterial biofilms and significant reductions in colony counts for <em>Staphylococcus aureus</em> and <em>Pseudomonas aeruginosa</em>. The nanoparticles also showed enhanced antibacterial efficacy against <em>S. aureus</em>, with minimal inhibitory concentrations (MIC) comparable to conventional antibiotics. By doubling the catechol content of PDA, PDA@PC amplified ROS production while mitigating oxidative stress, offering a safe and effective platform for treating resistant infections. This work highlights the potential of PDA@PC as a multifunctional, laser-activated nano therapy to replace traditional antibiotics and combat the global antimicrobial resistance crisis.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"270 ","pages":"Article 113222"},"PeriodicalIF":3.9,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144633623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of near-infrared polychromatic light in hyperglycemic human cell models: Toward improved diabetic wound healing","authors":"Simge Çıtak Demirez ,&nbsp;Tülay Selin Erkut ,&nbsp;Anıl Sera Çakmak ,&nbsp;Özer Aylin Gürpınar ,&nbsp;Menemşe Gümüşderelioğlu","doi":"10.1016/j.jphotobiol.2025.113224","DOIUrl":"10.1016/j.jphotobiol.2025.113224","url":null,"abstract":"<div><div>Diabetic ulcers are a serious complication of diabetes, often leading to reduced quality of life and, in many cases, limb amputation. These ulcers are characterized by impaired wound healing, primarily due to hyperglycemia-induced inflammation that disrupts normal cellular function. Given the limited effectiveness of conventional therapies, there is a critical need for alternative treatment strategies. This study investigated the therapeutic potential of photobiomodulation (PBM) using polychromatic light in the near-infrared range (600–1200 nm) on human cell types associated with diabetic wound healing, thereby extending previous research predominantly centered on monochromatic light sources. In vitro experiments were conducted using dermal fibroblasts, keratinocytes, and endothelial cells cultured under high-glucose conditions (25–150 mM) to simulate the diabetic environment. A glucose concentration of 150 mM was identified as optimal for modeling hyperglycemia, and PBM treatment was applied accordingly. The protocol consisted of irradiation at an intensity of 2.4 J/cm² per minute, delivered from a distance of 20 cm for 3 min on alternate days over a 7-day culture period. Under these hyperglycemic conditions, PBM significantly enhanced the proliferation and migration of dermal fibroblasts and keratinocytes, restoring these functions to levels comparable to normoglycemic controls. Furthermore, PBM increased fibronectin and collagen synthesis in both control and hyperglycemic groups. In endothelial cells, PBM improved tube formation, which was otherwise impaired under elevated glucose levels. Overall, these results indicate that PBM using a polychromatic light source may serve as a promising adjunctive therapy for enhancing wound healing in diabetic ulcers.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"270 ","pages":"Article 113224"},"PeriodicalIF":3.9,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144653039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A hydroxyl coumarin-chalcone-based fluorescent probe for sensing copper ions in plant and living cells","authors":"Keyi Li ,&nbsp;Yan Huang ,&nbsp;Yatong Sun ,&nbsp;Yiping Zhang ,&nbsp;Yan Zhang ,&nbsp;Baosheng Ren ,&nbsp;Duxia Cao","doi":"10.1016/j.jphotobiol.2025.113218","DOIUrl":"10.1016/j.jphotobiol.2025.113218","url":null,"abstract":"<div><div>Copper ions are essential trace elements for living organisms. However, its excessive levels in the liver and kidneys may lead to severe health issues. Therefore, developing effective fluorescent probes for detecting copper ions is crucial. A hydroxyl coumarin chalcone derivative featuring a hydroxyl substituent has been developed as a fluorescent probe for monitoring Cu²⁺. The probe can selectively detect Cu²⁺ in aqueous solutions, exhibiting a visible color change from red to light yellow to the naked eye. This is accompanied by a quenching of the red fluorescence, with a limit of detection of 11.2 μM. By utilizing Fourier transform infrared spectroscopy, fluorescence spectral changes upon alternating addition of Cu²⁺ and ethylene diamine tetraacetic acid, high-resolution mass spectrometry, and Job's plot analysis, along with a comparison of properties to a structurally analogous compound lacking a hydroxyl substituent, we propose that the probe engages in a 1:1 coordination reaction between its hydroxyl, adjacent carbonyl groups and Cu²⁺. The probe was successfully employed for the quantitative determination of Cu²⁺ content in a variety of real water, beverage and plant samples. Furthermore, the probe exhibits excellent biocompatibility, emitting bright fluorescence under confocal microscopy, which allows for the monitoring of intracellular Cu²⁺ level fluctuations.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"270 ","pages":"Article 113218"},"PeriodicalIF":3.9,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144588701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Femtosecond laser-induced myocardial incision: Preclinical study towards new tools for precision cardiac surgery","authors":"Ang Liu ,&nbsp;Tong Xia ,&nbsp;Siyuan Cao ,&nbsp;He Zhao ,&nbsp;Yubin Hou ,&nbsp;Xuejing Duan ,&nbsp;Li Li ,&nbsp;Ke Wang ,&nbsp;Pu Wang ,&nbsp;Chaowu Yan","doi":"10.1016/j.jphotobiol.2025.113221","DOIUrl":"10.1016/j.jphotobiol.2025.113221","url":null,"abstract":"<div><h3>Background</h3><div>Advanced precise laser techniques for myocardial tissue removal still need to be further explored. Femtosecond laser technology, renowned for its high precision and relatively minor collateral damage in biological tissue processing, shows significant promise for myocardial excision.</div></div><div><h3>Objective</h3><div>This study examined the effects of laser power and scanning speed on myocardial cutting outcomes, as well as to evaluate the feasibility and clinical application potential of femtosecond lasers in myocardial incision.</div></div><div><h3>Methods</h3><div>In this in vitro study using porcine hearts, cubic volumes (1.0 cm³) were laser-cut at varying powers (1.0 W to 5.0 W) and scanning speeds (1.0 mm/s to 4.0 mm/s). A 1064 nm femtosecond fiber laser emitting 179 fs duration pulses at a repetition rate of 500 kHz was used. Digital optical microscopy measured incision width, depth and thermal injury area. Statistical methods including correlation analysis and linear regression were applied to analyze the relationships between variables, and pathological examinations assessed tissue structure and injury extent.</div></div><div><h3>Results</h3><div>Laser power and scanning speed significantly influenced the cutting width, depth, and extent of thermal damage, with laser power played a decisive role andshowed a clear positive correlation with cutting width, depth, and thermal injury. Scanning speed primarily affected cutting efficiency, indirectly influenced cutting outcomes. Pathological examination revealed that increased laser power led to changes in thermal damage to myocardial tissue, characterized by enhanced eosinophilia of myocardial cell cytoplasm.</div></div><div><h3>Conclusions</h3><div>Femtosecond laser technology facilitates precise myocardial incision through meticulous modulation of power and scanning speed, validated exceptional safety and feasibility while highlighting substantial clinical potential. Further research is required to investigate related mechanisms.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"270 ","pages":"Article 113221"},"PeriodicalIF":3.9,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144633624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phthalocyanine-based nanosensitizers for enhanced sonodynamic therapy activity of hypoxic tumor with dual apoptosis/ferroptosis pathways","authors":"Xue-Yan Li,&nbsp;Jin Liu,&nbsp;Bi-Yuan Zheng,&nbsp;Mei-Rong Ke,&nbsp;Jian-Dong Huang","doi":"10.1016/j.jphotobiol.2025.113211","DOIUrl":"10.1016/j.jphotobiol.2025.113211","url":null,"abstract":"<div><div>The development of efficient and versatile theranostic agents based on a single molecule for sonodynamic therapy (SDT) of hypoxic tumor remains a formidable challenge. Herein, we present a spatiotemporally controllable oxygen-releasing nanoliposomal sonosensitizer (PcA-PFO@FLPs) based on a multifunctional phthalocyanine derivative, which integrates dual-modality imaging guidance for enhanced SDT of hypoxic tumors. The perfluoropentadecyl ether oxygen reservoir enables PcA-PFO@FLPs to exhibit ultrasound-responsive oxygen release, enhancing reactive oxygen species (ROS) generation at both solution and cellular levels, thereby demonstrating potent sonodynamic activity (IC₅₀ = 1.55 μM) against 4 T1 cells. Notably, the nanosonosensitizer maintains comparable ROS production efficiency in hypoxic and normoxic cancer cells. This robust ROS generation not only triggers mitochondrial membrane depolarization (89.5 % of depolarization rate) and apoptosis but also induces iron-free ferroptosis through lipid peroxidation accumulation and glutathione depletion (65 % of depletion). In vivo fluorescence and photoacoustic imaging confirmed tumor-selective retention with rapid renal clearance, enabling efficient tumor suppression in murine models while maintaining biosafety. Thus, this work establishes a new paradigm for developing versatile nanosonosensitizers for SDT of hypoxic tumors.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"270 ","pages":"Article 113211"},"PeriodicalIF":3.9,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144570354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photobiomodulation mitigates DHT-induced apoptosis in dermal papilla cells via mitochondrial rescue and Wnt/TGF-β/BMP signaling modulation","authors":"Yi Ren ,&nbsp;Xiaojing Miao ,&nbsp;Hui Jiang ,&nbsp;Angze Li ,&nbsp;Longfei Huo ,&nbsp;Xuran Zhang ,&nbsp;Qiqi Fu ,&nbsp;Jiali Yang ,&nbsp;Jing Tian ,&nbsp;Muqing Liu","doi":"10.1016/j.jphotobiol.2025.113210","DOIUrl":"10.1016/j.jphotobiol.2025.113210","url":null,"abstract":"<div><div>Current therapeutic interventions for androgenetic alopecia (AGA) are hindered by limited efficacy and adverse side effects. Photobiomodulation (PBM) has emerged as a promising non-invasive alternative, demonstrating preliminary potential for hair follicle stimulation. However, its precise therapeutic mechanisms and optimal treatment parameters require systematic investigation. In the present study, we established an <em>in vitro</em> AGA model using dihydrotestosterone (DHT)-treated human dermal papilla cells (DPCs, 0–100 μM) to evaluate PBM efficacy across continuous wave (CW) and pulsed wave (PW) modes, enabling mechanistic and therapeutic assessment. Key findings revealed that the impact of PBM was highly sensitive to DHT concentration. At lower concentrations of DHT (0–50 μM), PBM therapy successfully improved mitochondrial function, reduced apoptosis, increased alkaline phosphatase activity, stimulated lactate dehydrogenase release, and boosted cell migration. These beneficial effects were particularly notable under 8 J/cm² and 8 mW/cm² (CW mode), as well as 8 J/cm², 10 mW/cm² (peak irradiance), 500 Hz, and 80 % duty cycle under PW mode. However, these protective effects were substantially attenuated at higher DHT concentrations (100 μM). Mechanistically, PW PBM exerted dual anti-apoptotic and anti-androgenic effects through multi-pathway modulation: It activated the Wnt/β-catenin pathway while concurrently suppressing <em>BMP</em> and <em>TGF</em>-<em>β</em> signaling cascades. This investigation elucidates the molecular mechanisms by which PBM inhibits DHT-induced apoptosis in DPCs. Furthermore, it demonstrates that the therapeutic efficacy of PBM is significantly mitigated under hyperandrogenic conditions. Overall, our findings provide critical insights for optimizing light-based therapeutic strategies and advancing clinical translation of PBM for AGA management.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"270 ","pages":"Article 113210"},"PeriodicalIF":3.9,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144557490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photobiomodulation mediates endoplasmic reticulum-mitochondria contact and ameliorates lipotoxicity in MASLD via Mfn2 upregulation","authors":"Zhixuan Jiang ,&nbsp;Shan Wu ,&nbsp;Shengzhe Zhou ,&nbsp;Hongjie Zheng ,&nbsp;Yubing Bai ,&nbsp;Yiqiu Zhang ,&nbsp;Min Yao","doi":"10.1016/j.jphotobiol.2025.113209","DOIUrl":"10.1016/j.jphotobiol.2025.113209","url":null,"abstract":"<div><div>The disruption of mitochondria associated membranes (MAMs) is involved in the pathogenesis of metabolic dysfunction-associated steatotic liver disease (MASLD) by modulating endoplasmic reticulum stress (ERS) and mitochondrial malfunction induced by lipotoxicity. Photobiomodulation (PBM), as a non-invasive physical therapy, has been demonstrated to improve cellular metabolism in various diseases. Here we found that PBM with 650 nm ameliorated lipid accumulation and liver injury in high-fat-diet-fed mice. Moreover, MAMs integrity was restored in liver tissues of MASLD after PBM. Correspondingly, PBM enhanced mitochondria-ER colocalization and improved mitochondrial homeostasis in fatty-acid-treated HepG2 cells. Mechanically, Mfn2 expression was selectively elevated by PBM, accompanied by downregulation of PERK, p-PERK, and CHOP. The beneficial effects of PBM were diminished by <em>Mfn2</em> knockdown, while PERK activity regulated oxidative stress without altering MAMs formation. Thus, PBM relieves lipotoxicity in MASLD by enhancing MAMs integrity via the Mfn2/PERK/CHOP pathway. Our findings may provide evidence for noninvasive physical light therapeutics for lifestyle-related metabolic diseases.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"270 ","pages":"Article 113209"},"PeriodicalIF":3.9,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144570626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}