Journal of photochemistry and photobiology. B, Biology最新文献

{"title":"Effects of photobiomodulation on human hair dermal papilla cells with various light modes and light parameters","authors":"Yi Ren ,&nbsp;Angze Li ,&nbsp;Xiaojing Miao ,&nbsp;Longfei Huo ,&nbsp;Haokuan Qin ,&nbsp;Hui Jiang ,&nbsp;Muqing Liu","doi":"10.1016/j.jphotobiol.2024.113080","DOIUrl":"10.1016/j.jphotobiol.2024.113080","url":null,"abstract":"<div><div>Androgenetic alopecia (AGA) is a prevalent hair loss disorder and influenced by genetic, hormonal, and environmental factors. Minoxidil and finasteride have been widely used for treating AGA. However, the side effects associated with these drugs often lead to poor patient compliance. In contrast, photobiomodulation (PBM), due to its safety and non-invasiveness, holds promising prospects for use. Although the promoting effects of PBM on AGA have been reported, the mechanisms by which PBM affects dermal papilla cells (DPCs) remain largely unknown. Hence, this study explored the impacts of both continuous wave (CW) and pulsed wave (PW) PBM on DPCs, and revealed the underlying actions of light parameters in PBM. Orthogonal experiments were conducted to evaluate the effects of CW PBM on DPCs at varying irradiances and doses, indicating that irradiance was the crucial parameter, as well as cell viability and proliferation were maximized at 8 mW/cm² and 8 J/cm². For PW PBM, response surface methodology was employed to determine the influences of duty cycles, frequencies, and doses. The findings highlighted frequency as a primary factor, with the optimum cell viability observed at peak irradiance 10 mW/cm², duty cycle 80 %, 500 Hz, and 8.8 J/cm². Notably, PBM could enhance cell viability, proliferation, and migration in DPCs by activating the Wnt/β-Catenin signaling and suppressing Transforming Growth Factor signaling, particularly when applied in pulsed mode. Overall, this study determined the key light parameters that influence PBM effectiveness, further identified the optimal light conditions, and preliminary revealed into the mechanisms of PBM in DPCs, highlighting that PW PBM may be a competitive therapeutic option for alleviating AGA in the future.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"262 ","pages":"Article 113080"},"PeriodicalIF":3.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liquid phase preparation and characterization of MgO nanoparticles and their butchery activities against dental bacterial pathogens and human cervical cancer cell line","authors":"Thamizharasan Sambandam ,&nbsp;Gurunathan Karuppasamy ,&nbsp;Govindhan Perumal ,&nbsp;Edison Chandraseelan Rajasingh","doi":"10.1016/j.jphotobiol.2024.113084","DOIUrl":"10.1016/j.jphotobiol.2024.113084","url":null,"abstract":"<div><div>A novel method for synthesizing nanomaterials involves microbial or phytochemical nano-factories, which offer an eco-friendly, cost-effective, and reliable approach to producing clean and reproducible products. In this study, magnesium oxide nanoparticles (MgO NPs) were synthesized using <em>Avicennia marina</em>, a marine plant, as both a nucleation and stabilizing agent. The MgO NPs were characterized for crystallinity, cut-off wavelength, morphology, thermal stability, and surface properties using XRD, EDX, BET, UV–Visible spectroscopy, DLS, zeta potential analysis, SEM, TEM, TGA/DTA, and PL spectroscopy. Using the Brunauer-Emmett-Teller (BET) method, the specific surface area of the MgO nanoparticles was determined to be 97.248 m²/g. The optical band gap energy of the MgO nanoparticles that corresponded to the highest absorption peak was determined to be 5.8 eV. Additionally, the antimicrobial, scavenging, and antioxidant activities of the MgO NPs were evaluated against dental bacterial pathogens. To explore anticancer potential, the MgO NPs were tested on a cervical cancer cell line (HeLa) using an MTT assay, which revealed that cytotoxicity increased with higher MgO NP concentrations. MgO nanoparticles were applied at concentrations of 25 μg/mL, 50 μg/mL, 75 μg/mL, and 100 μg/mL to inhibit the proliferation of cancer cell lines. The cytotoxicity observed at an IC50 concentration of 56.54 μg/mL is primarily influenced by factors such as the small size, increased surface-to-volume ratio, oxygen vacancies, and the morphology of the nanoparticles. This interdisciplinary study contributes to the understanding of MgO NPs and their multifunctional potential in combating dental infections and cervical cancer.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"262 ","pages":"Article 113084"},"PeriodicalIF":3.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro photoprotective efficacy and photostability of synthesized star-shaped ZnO nanoaggregates associated with ethylhexyl methoxycinnamate and butyl methoxydibenzoylmethane","authors":"Gustavo Teixeira Machado ,&nbsp;Caio Rui Chiabai ,&nbsp;Mariana Santos Pinheiro ,&nbsp;Claudinéia Aparecida Sales de Oliveira Pinto ,&nbsp;André Rolim Baby ,&nbsp;George Ricardo Santana Andrade ,&nbsp;Fabiana Vieira Lima Solino Pessoa","doi":"10.1016/j.jphotobiol.2024.113068","DOIUrl":"10.1016/j.jphotobiol.2024.113068","url":null,"abstract":"<div><div>The heightened susceptibility to skin cancer correlates with exposure to ultraviolet (UV) radiation, which can induce various cutaneous injuries. Inorganic UV filters, like zinc oxide (ZnO), are extensively utilized in sunscreens owing to their capacity to scatter and reflect UV radiation. The efficacy of inorganic UV filters can be augmented across a wider spectrum through synergistic combinations with other active compounds, such as organic UV filters. In this study, we synthesized and evaluated the <em>in vitro</em> effectiveness of star-shaped ZnO nanoaggregates. The samples were obtained employing a simple and greener precipitation method in an aqueous solution. The synthesized ZnO nanoaggregates were characterized through X-ray diffraction, UV/Vis spectroscopy, and transmission electron microscopy. The characterization suggests the aggregation of nanocrystals, with hexagonal wurtzite geometry, enabling the formation of star-shaped particles. The irregular (non-flat) surface and high surface area combined with light absorption in the UVA region make the material susceptible to the application of sun protection. Sunscreens formulated with the synthesized ZnO_ng in conjunction with ethylhexyl p-methoxycinnamate (EHMC) and/or butyl methoxydibenzoylmethane (BMDBM) were evaluated for <em>in vitro</em> sun protection factor (SPF) and critical wavelength (cλ) using diffuse reflectance spectrophotometry with an integrated sphere. Photostability assessments were also conducted using artificial UV radiation. Sunscreen formulations containing ZnO_ng in combination with EHMC and BMDBM exhibited a substantial increase in <em>in vitro</em> SPF, approximately 990 % (from SPF 26 to 285). Furthermore, the synthesized ZnO_ng demonstrated higher <em>in vitro</em> efficacy compared to commercial ZnO active ingredients. Although all samples experienced reductions in SPF values during the photostability assay, ZnO_ng + EHMC + BMDBM retained a broad-spectrum profile (SPF &gt; 15 and cλ &gt; 370 nm). Based on the distinctive properties and <em>in vitro</em> performance of star-shaped ZnO_ng, we propose that this synthesized inorganic UV filter could serve as an alternative for enhancing the SPF of sunscreen systems while reducing the concentrations of organic UV filters.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"261 ","pages":"Article 113068"},"PeriodicalIF":3.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142743388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A tumor-pH-responsive phthalocyanine as activatable type I photosensitizer for improved photodynamic immunotherapy","authors":"Ling Zhang ,&nbsp;Rong-Bin Que ,&nbsp;Ting-Ting Ke,&nbsp;Chao Wang,&nbsp;Wei Xie,&nbsp;Hong-Jie Sun,&nbsp;Bi-Yuan Zheng,&nbsp;Mei-Rong Ke,&nbsp;Jian-Dong Huang,&nbsp;Xingshu Li","doi":"10.1016/j.jphotobiol.2024.113067","DOIUrl":"10.1016/j.jphotobiol.2024.113067","url":null,"abstract":"<div><div>The development of a simple drug formulation capable of achieving both activatable type I photoreaction and tumor-responsive release of immunomodulator is crucial for advancing photodynamic immunotherapy (PDIT). Herein, we present a nanostructured photosensitizer (NP5) that is activated by the acidic tumor microenvironment to produce type I reactive oxygen species (ROS) under light irradiation and release the immunomodulator demethylcantharidin (DMC) for PDIT. The NP5 is formed by self-assembly of a versatile phthalocyanine molecule which is composed of DMC and phthalocyanine linked <em>via</em> a pH-responsive amide bond. NP5 produces minimal ROS under light irradiation at the condition of pH 7.4. However, NP5 can release DMC at the condition of pH 6.5 and concurrently trigger type I photoreactions. The results of <em>in vivo</em> experiments indicate that NP5-mediated PDIT induce the increase of cytotoxic T lymphocytes and decrease of regulatory T lymphocytes, which can effectively inhibit the bilateral tumor growth. This work is anticipated to serve as a reference for the development of innovative agents for precise PDIT of hypoxic tumors.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"261 ","pages":"Article 113067"},"PeriodicalIF":3.9,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ct-DNA compaction by nanoparticles formed by silica and gemini surfactants having hydroxyl group substituted spacers: In vitro, in vivo, and ex vivo gene uptake to cancer cells","authors":"Shalini Dyagala ,&nbsp;Sayantan Halder ,&nbsp;Rishika Aggrawal ,&nbsp;Milan Paul ,&nbsp;Vinod K Aswal ,&nbsp;Swati Biswas ,&nbsp;Subit Kumar Saha","doi":"10.1016/j.jphotobiol.2024.113066","DOIUrl":"10.1016/j.jphotobiol.2024.113066","url":null,"abstract":"<div><div>Hybrid nanoparticles formed by Silica (SiO₂) coated with cationic gemini surfactants with variable hydroxyl group substituted spacers, 12-4(OH)-12,2Br⁻ and 12-4(OH)₂-12,2Br⁻ have shown a great extent of compaction of calf thymus DNA (ct-DNA) compared to conventional counterpart cationic surfactant, dodecyl trimethylammonium bromide (DTAB). Study shows not only the hydrophobicity of the spacer but also the hydrogen bonding interactions between the hydroxyl group substituted spacer and DNA have a great role in DNA compaction. 12-4(OH)₂-12,2Br⁻ is more efficient in compacting ct-DNA compared to 12-4(OH)-12,2Br⁻ due to the stronger binding of the former with ct-DNA than the latter. While 12-4(OH)-12,2Br⁻ makes 50 % ct-DNA compaction at its 0.63 μM concentration in the presence of SiO₂ nanoparticles, the same % of compaction can be achieved at a concentration as low as 0.25 μM of 12-4(OH)₂-12,2Br⁻. However, DTAB makes 50 % ct-DNA compaction at a concentration as high as 7.00 μM under the same condition. Therefore, the present systems address the very common challenge, <em>i.e.</em>, cytotoxicity due to cationic surfactants. The system of 12-4(OH)₂-12,2Br⁻ coated SiO₂ nanoparticles displays the maximum cell viability (≥90 %), causing the least cell death in the mouse fibroblast cells (NIH3T3) cell lines compared to the cell viability of ≤80 % for DTAB. 12-4(OH)₂-12,2Br⁻ coated SiO₂ nanoparticles system has presented excellent <em>in vitro</em> cellular uptake of genes on mouse mammary gland adenocarcinoma (4T1) cells after incubating for 3 h and 6 h. <em>In vivo</em> study shows that 12-4(OH)₂-12,2Br⁻ coated SiO₂ nanoparticles system takes the highest amount of ct-DNA in cells and tumors in a time-dependent manner. The <em>ex vivo</em> studies using different organs of the mice demonstrate that the tumor sites in the breast of the mice are most affected by these formulations. Cytotoxicity assays and cellular uptake studies suggest that the present systems can be used for potential applications for gene delivery and oncological therapies.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"261 ","pages":"Article 113066"},"PeriodicalIF":3.9,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142663986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of highly biocompatible SiO2@Au-BSA nanoconjugates: Towards a promising thermal therapy route","authors":"Rubén Gutiérrez-Fuentes ,&nbsp;Libertad Juárez-Santacruz ,&nbsp;Issis Claudette Romero-Ibarra ,&nbsp;José Luis Jiménez-Pérez ,&nbsp;Angel Netzahual-Lopantzi","doi":"10.1016/j.jphotobiol.2024.113064","DOIUrl":"10.1016/j.jphotobiol.2024.113064","url":null,"abstract":"<div><div>SiO₂@Au nanoshells have gained relevance in recent years, especially in biomedical areas, acting as thermal therapy agents due to their high capacity to absorb light and transform it into heat that increases the temperature of the medium. Therefore, it is important to develop methodological strategies to obtain stable, highly specific and biocompatible nanoparticles. In this work, the synthesis of core-shell structures based on SiO₂@Au is reported, where the growth a thin shell ⁓ 46 nm on silica platform was possible. Subsequently, optimal conditions were developed for the binding of a bovine serum albumin (BSA) protein using a thiolated linker such as mercaptoethanol. Likewise, the photothermal conversion capacity was investigated using thermal lens spectroscopy. Thermal diffusivity values were reported for the first time during the conjugation process of gold nanoshells, where an increase of 37.5 % was recorded as the conjugation was completed. Finally, the cytotoxic potential of the developed nanoconjugates was evaluated through their hemolytic rate in human red blood cells. The findings suggest high hemocompatibility of the SiO₂@Au-BSA complex because they did not cause significant oxidative stress and are classified as nonhemolytic. Therefore, in this work we propose a synthesis route for a thermal agent based on SiO₂@Au and bovine serum albumin, highly biocompatible and with high photothermal conversion. The results of this work aim to clarify the safety of using gold nanoshells as a thermal therapy agent.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"261 ","pages":"Article 113064"},"PeriodicalIF":3.9,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiome shifts elicited by ornamental lighting of granite facades identified by MinION sequencing","authors":"Anxo Méndez ,&nbsp;Francesca Maisto ,&nbsp;Jelena Pavlović ,&nbsp;Magdaléna Rusková ,&nbsp;Domenico Pangallo ,&nbsp;Patricia Sanmartín","doi":"10.1016/j.jphotobiol.2024.113065","DOIUrl":"10.1016/j.jphotobiol.2024.113065","url":null,"abstract":"<div><div>Night-time outdoor illumination in combination with natural sunlight can influence the visible phototrophic colonizers (mainly algae) growing on stone facades; however, the effects on the microbiome (invisible to the naked eye) are not clear. The presence of stone-dwelling microbes, such as bacteria, diatoms, fungi, viruses and archaea, drives further biological colonization, which may exacerbate the biodeterioration of substrates. Considering the microbiome is therefore important for conservation of the built heritage. The impact of the following types of lighting on the relative abundance and diversity of the microbiome on granite ashlars was evaluated in a year-long outdoor pilot study: no lighting; lighting with a metal halide lamp (a traditional lighting system currently used to illuminate monuments); and lighting with a novel LED lamp (an environmentally sound prototype lamp with a biostatic effect, halting biological colonization by phototrophs, currently under trial). Culturable fractions of microbiome and whole-genome sequencing by metabarcoding with Oxford Nanopore Sequencing (MinION) was conducted for bacteria and fungi in order to complement both community characterization strategies. In addition, the possible biodeteriorative profiles of the isolated strains, relative to calcium carbonate precipitation/solubilisation and iron oxidation/reduction, were investigated by plate assays. Alpha and beta diversity indexes were also determined, along with the abundance of biocide and antibiotic resistance genes. Culture-dependent microbiological analysis failed to properly show changes in community composition, for which metagenomic approaches like MinION are better suited. Thus, MinION analysis identified shifts in the granite microbiome elicited by ornamental lighting. The novel LED lamp with the biostatic effect on phototrophs caused an increase in the diversity of bacteria and fungi. In this case, the microbiome was more similar to that in the unlit samples. In the samples illuminated by the metal halide lamp, dominance of bacteria was favoured and the presence of fungi was negligible.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"261 ","pages":"Article 113065"},"PeriodicalIF":3.9,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The interplay between LHCSR and PSBS proteins provides photoprotection in Chlamydomonas reinhardtii pgr5 mutant under high light","authors":"Ranay Mohan Yadav ,&nbsp;Nisha Chouhan ,&nbsp;Jerome Xavier Gunasekaran ,&nbsp;Sai Kiran Madireddi ,&nbsp;Aparna Nerusu ,&nbsp;Rajagopal Subramanyam","doi":"10.1016/j.jphotobiol.2024.113060","DOIUrl":"10.1016/j.jphotobiol.2024.113060","url":null,"abstract":"<div><div>Cyclic electron transport (CET) is a vital alternative route that protects against photodamage and aids in energy production. This process depends on proton gradient regulation 5 (PGR5) and PGRL1-dependent pathways associated with CET. The exact roles of these proteins in photosystem I photochemistry under prolonged high light conditions are not fully understood. Continuous light adaptation hinges on two critical mechanisms: alterations in the proton motive force (pmf) and adjustments in the ratio of proteins activated by high light that dissipate excess light through non-photochemical quenching (NPQ). To explore this, we studied <em>pgrl1</em> and <em>pgr5</em> mutants to gauge their roles in balancing photochemistry and photoacclimation. These mutants showed inhibited growth, reduced photosynthetic efficiency, and a lowered pmf, leading to diminished non-photochemical energy quenching (qE) under high light. Prolonged high light exposure slowed down unregulated energy losses Y(NO), and relaxation helped regulate photosynthetic activity by increasing photoinhibitory quenching (qI), thus preventing further damage to the photosystem. The precise balance between the two pmf components, ΔpH and Δψ, is critical for controlling photochemistry and photoacclimation, yet remains elusive. In <em>pgr5</em> reduced pmf led to an accumulation of cytochrome <em>b</em>₆<em>f</em> under high light, and a decrease in the ΔpH component and increased the Δψ component's role in photosynthetic acclimation. Notably, light-harvesting complex stress response protein 3 (LHCSR3) showed decreased expression in <em>pgrl1</em>, whereas <em>pgr5</em> exhibited no expression of both LHCSR3 and LHCSR1 under high-light conditions. Moreover, continuous increase in PSBS protein accumulation in <em>pgr5</em> suggests enhanced photoprotection in the absence of LHCSR3 under high light. The study provides significant insights into how CET regulates photoprotective proteins LHCSR and PSBS, influencing Chlamydomonas' survival strategies.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"261 ","pages":"Article 113060"},"PeriodicalIF":3.9,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142639259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro and in vivo investigation of the inhibitory effects of Sinoporphyrin sodium-mediated Sonodynamic therapy on human oral squamous cell carcinoma","authors":"Guogang Dong ,&nbsp;Limin Jia ,&nbsp;Shuhua Gao ,&nbsp;Monan Lin ,&nbsp;Ruilin Wang ,&nbsp;Fuyu Yang ,&nbsp;Juanjuan Ruan ,&nbsp;Yanhong Lv","doi":"10.1016/j.jphotobiol.2024.113061","DOIUrl":"10.1016/j.jphotobiol.2024.113061","url":null,"abstract":"<div><h3>Objective</h3><div>Sonodynamic therapy (SDT) is an innovative, non-invasive approach to cancer treatment, by using low-intensity ultrasound to trigger the activation of sonosensitizers localized within cancerous cells. This current study aimed to explore the therapeutic efficacy of a new sonosensitizer, Sinoporphyrin Sodium (DVDMS), under ultrasound irradiation, against oral squamous cell carcinoma (OSCC)-derived SCC-154 cells, both in vitro and in vivo.</div></div><div><h3>Methods</h3><div>Fluorescence spectra, cytotoxicity assessments, uptake mechanisms, and subcellular distributions of DVDMS within the SCC-154 cell line were detected. Additionally, the study comprehensively assessed the antitumor effect, oxidative stress responses, apoptosis, apoptosis-related proteins, autophagic processes, and ultrastructural changes in SCC-154 cells, both in vitro and in vivo, subsequent to treatment with low-intensity ultrasound (at 1.0 MHz, 1 W/cm² in vitro and 3 W/cm² in vivo) in conjunction with DVDMS also being examined.</div></div><div><h3>Results</h3><div>The findings indicate that SCC-154 cells exhibit heightened sensitivity to DVDMS compared to SAS and HSC-3 cell lines. Within SCC-154 cells, DVDMS primarily localizes within the mitochondria and lysosomes. DVDMS-based SDT significantly increased the intracellular levels of reactive oxygen species (ROS), induced morphological changes such as mitochondrial swelling and formation of autolysosomes, and exhibited a notable dose-dependent reduction in cell viability in vitro. Also, DVDMS-SDT demonstrated significant inhibition of xenograft growth without discernible adverse effects. Mechanistically, DVDMS-SDT upregulated Bax expression while downregulating Bcl-2 expression, which led to the Bax/Bcl-2 ratio and induced autophagy.</div></div><div><h3>Conclusion</h3><div>DVDMS-SDT triggers mitochondrial-dependent apoptosis in SCC-154 cells, unlike 5-ALA and protoporphyrin IX (PpIX). Also, the combination of DVDMS with ultrasound stimulation induces autophagy, with the onset of autophagic processes preceding apoptosis.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"261 ","pages":"Article 113061"},"PeriodicalIF":3.9,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142622039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of heavy-atom-free thienoisoindigo dye as near-infrared photosensitizer for type I photodynamic therapy and photoacoustic imaging","authors":"Feng Zhang ,&nbsp;Hao Cai ,&nbsp;Leichen Wang ,&nbsp;Jinjun Shao","doi":"10.1016/j.jphotobiol.2024.113052","DOIUrl":"10.1016/j.jphotobiol.2024.113052","url":null,"abstract":"<div><div>Thienoisoindigo (TIIG) has been extensively employed as promising building block of near-infrared (NIR) dyes and organic semiconductor materials. Herein, heavy-atom-free TIIG-based NIR dye TIIGTPA is reported as photosensitizer for combinational photodynamic and photothermal therapy and photoacoustic imaging (PAI). By introducing two methoxy-substituted triphenylamines as the rotors and electron donors at the periphery sites of the electron-deficient TIIG core, dye TIIGTPA featuring Donor-Acceptor-Donor (D-A<img>D) structure is constructed with intensive NIR absorption. Through co-assembly with amphipathic F-127, water-soluble TIIGTPA NPs were prepared with good superoxide anion radical (O₂^-•) production and high photothermal conversion efficiency (PCE) of 59.0 % under 730 nm photoirradiation. Additionally, the excellent photothermal effect enabled a superior photoacoustic response for tumor blood vessel visualization through PAI. All results indicated the favorable potential of TIIGTPA NPs for PAI-mediated combinational phototherapy.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"261 ","pages":"Article 113052"},"PeriodicalIF":3.9,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142605059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}