Journal of photochemistry and photobiology. B, Biology最新文献

{"title":"Photodynamic ablation of floating lung cancer cells using PVA and TPGS emulsified PLGA nanoparticles loaded with pyropheophorbide-a","authors":"Sasivimon Pramual ,&nbsp;Philippe Arnoux ,&nbsp;Kriengsak Lirdprapamongkol ,&nbsp;Céline Frochot ,&nbsp;Valérie Jouan-Hureaux ,&nbsp;Muriel Barberi-Heyob ,&nbsp;Jisnuson Svasti","doi":"10.1016/j.jphotobiol.2025.113318","DOIUrl":"10.1016/j.jphotobiol.2025.113318","url":null,"abstract":"<div><div>Metastasis or the spread of cancer cells to other tissues is a hallmark that leads to the majority of cancer-related deaths worldwide. When metastasizing cancer cells invade into the bloodstream, they become floating cells, also known as circulating tumor cells (CTCs), which can lead to the development of metastasis-associated multidrug resistance in advanced cancer patients. Eradication of CTCs has received much attention as a strategy for preventing metastasis. Photodynamic therapy (PDT) has attracted growing interest as a minimally invasive approach for cancer treatment. Pyropheophorbide-a (PPa) is photosensitizer with advantages of relatively high wavelength absorption and high extinction coefficient; however, it has limited PDT therapeutic benefits due to poor solubility. This work aimed to employ PDT for killing CTCs by utilizing PVA and TPGS coated PLGA nanoparticles, loaded with PPa. The PPa-entrapped PLGA nanoparticles (PPa-NPs) exhibited a spherical morphology under TEM with an average size of 124.9 ± 2.3 nm and a zeta potential value of −32.0 ± 1.4 mV. The PPa-NPs enhanced singlet oxygen generation in water upon light activation. PPa-NPs successfully delivered PPa into A549 floating cells under CTC-mimicking conditions, with 21-fold increase in intracellular PPa accumulation when compared to free PPa treatment. After red light excitation, intracellular ROS level was elevated in PPa-NPs treated floating cells, in a dose-dependent manner, and correlated with photocytotoxic effect of PPa-NPs in the floating cells. Our results demonstrate that PVA and TPGS stabilized PLGA NPs efficiently preserved the photophysical properties of PPa for eradicating CTCs by PDT with red light activation.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"274 ","pages":"Article 113318"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145661339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of red light-emitting diode therapy in imiquimod-induced psoriasis in mice","authors":"Mab P. Corrêa ,&nbsp;Rebeca D. Correia-Silva ,&nbsp;Diego D. Santos ,&nbsp;Nathália Rodrigues-Silva ,&nbsp;Pâmela P. Borges ,&nbsp;Silvana Sandri ,&nbsp;Karin V. Greco ,&nbsp;Cristiane D. Gil","doi":"10.1016/j.jphotobiol.2025.113333","DOIUrl":"10.1016/j.jphotobiol.2025.113333","url":null,"abstract":"<div><div>Growing evidence supports the therapeutic potential of phototherapies, particularly light-emitting diodes (LEDs), in modulating inflammation and restoring tissue homeostasis. Here, we evaluated the effects of red LED irradiation (660 nm) in a murine model of psoriasis induced by imiquimod (IMQ). C57BL/6 mice were treated with IMQ for 12 consecutive days and exposed to LED on alternate days from day 4, totalling five sessions. Macroscopic evaluation showed a significant reduction in skin-fold thickness and Psoriasis Area and Severity Index (PASI) scores in LED-treated mice. Histological analyses confirmed that IMQ induced epidermal thickening, erythema, mast cell infiltration, and collagen alterations. LED exposure attenuated epidermal changes (<em>p</em> &lt; 0.0001, IMQ + LED versus IMQ), reduced mast cell numbers (<em>p</em> &lt; 0.05, IMQ + LED versus IMQ), and modulated collagen fiber distribution, although it did not reverse dermal thickening. At the systemic level, IMQ increased spleen weight and white pulp expansion, effects not prevented by LED. In skin samples, IMQ markedly elevated interleukin (IL)-1β and tumor necrosis factor (TNF)-α levels, which were significantly reduced by LED treatment (<em>p</em> &lt; 0.05). In plasma, IL-22 was elevated in IMQ-treated animals but decreased in the IMQ + LED group (p &lt; 0.05), while no significant changes were detected for IL-1β, IL-17A, IL-23, TNF-α, or macrophage inflammatory protein (MIP)-3α. Together, these results suggest that red LED phototherapy reduces clinical severity and partially modulates both local and systemic inflammation in IMQ-induced psoriasis, supporting its potential as a complementary therapeutic approach.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"274 ","pages":"Article 113333"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145714737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gd(III) and Fe(III) ion crosslinked hyaluronic acid microgels composites embedding hetero atom doped carbon quantum dots render photodynamic therapy with improved bioimaging capability","authors":"Selin Sagbas Suner ,&nbsp;Mehtap Sahiner ,&nbsp;Evrim Umut ,&nbsp;Nurettin Sahiner","doi":"10.1016/j.jphotobiol.2025.113342","DOIUrl":"10.1016/j.jphotobiol.2025.113342","url":null,"abstract":"<div><div>In this study, we report the development of multifunctional CQ-dot@HA-Gd/Fe(III) microgels that can be readily simultaneously used in fluorescence/MR dual-mode imaging and photodynamic therapy as theragnostic agents. Nitrogen (N-) and sulfur (S-) heteroatom-doped carbon quantum dots (CQ-dot) were prepared in one step microwave treatment within 3 min as a fluorescence and photoinduced antipathogenic nanomaterial. The N/S-doped CQ-dots were spherical shaped and &lt; 50 nm via TEM images and showed high fluorescence intensity with 420 nm emission wavelength at maximum λ_ex:350 nm. The N/S-doped CQ-dots were embedded into ionically crosslinked hyaluronic acid (HA) microgels, employing trivalent metal ions Gd(III) or Fe(III) ions. The prepared CQ-dot@HA-Gd/Fe(III) microgels &lt;5 mm size range are injectable for possible intravenous administration and possess high fluorescent properties. The isoelectric point (IEP) of CQ-dot@HA-Gd and CQ-dot@HA-Fe(III) microgels was determined as pH 1.45. The CQ-dot@HA-Gd/Fe(III) microgels exhibit excellent hemocompatibility without causing noticeable hemolysis and blood clotting at concentrations up to 500 mg/mL. Furthermore, the toxicity of CQ-dot@HA-Gd/Fe(III) microgels on L929 fibroblast cells was found as 100 mg/mL concentration and provide brilliant cell imaging under DAPI filter without any fluorescence dye. Also, the CQ-dot@HA-Gd/Fe(III) microgel suspension afforded great MRI contrast enhancement ability. Photoinduced anticancer activity was observed for CQ-dot@HA-Gd/Fe(III) microgels even at 50 mg/mL against SK-MEL 30 melanoma cells under UV-A light treatment for 30 min. In addition, high reactive oxygen species (ROS) generation was obtained for the pathogenic bacteria cells by light-sensitive CQ-dot@HA-Gd/Fe(III) microgels upon 30 min UV-A light treatment that triggered the destruction of the <em>Staphylococcus aureus</em> (ATCC 6538).</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"274 ","pages":"Article 113342"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145797081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"C-Phycocyanin-mediated photoactivation versus Er: YAG laser irradiation for ceramic bracket surface conditioning: Influence on bonding efficacy of sepiolite nanoparticle-modified adhesive","authors":"Salem Almoammar ,&nbsp;Muhammad Abdullah Kamran ,&nbsp;Abdulrahman Alshehri ,&nbsp;Wael Awadh ,&nbsp;Amyrah Musafir Alshehrany ,&nbsp;Ibrahim Alshahrani","doi":"10.1016/j.jphotobiol.2025.113346","DOIUrl":"10.1016/j.jphotobiol.2025.113346","url":null,"abstract":"<div><h3>Aim</h3><div>The current study investigated the potential of Er: YAG laser and C-Phycocyanin (C-PC) mediated photoactivation (PA) as alternatives to hydrofluoric acid (HFA) for the preparation of ceramic bracket surfaces before bonding. Additionally, the study also evaluated the incorporation of sepiolite nanoparticles (SepNP) into the adhesive could enhance the bonding efficacy of the conditioned ceramic bracket to enamel.</div></div><div><h3>Methodology</h3><div>One-twenty ceramic brackets were allocated into four distinct treatment groups: untreated, HFA etched, Er: YAG laser treated, and C-PC-PA. Each group was further divided, with half of the brackets bonded using a standard adhesive and the other half using an adhesive augmented with 1 % SepNPs. Surface roughness (Ra) was quantified utilizing a profilometer, and the pretreated ceramic bracket bases were scrutinized via scanning electron microscopy (SEM). Following the aging process of the samples, the force required to debond the ceramic bracket from the enamel was evaluated using a universal testing machine (UTM). The failure mode was documented using the adhesive remnant index (ARI), and the degree of conversion (DC) of both modified and unmodified adhesives was subsequently assessed.</div></div><div><h3>Results</h3><div>The laser-treated roughened surfaces (1167.43 ± 6.81 μm) demonstrated comparable efficacy to those etched with HFA (1158.74 ± 8.54 μm). In contrast, the C-PC-PA method resulted in minimal surface roughening (735.47 ± 5.69 μm), showing no significant improvement over untreated brackets. The pattern of bond strength was consistent with these findings—both the laser and acid-treated groups exhibited strong bonding (8.51–9.60 MPa), whereas the photoactivation and untreated groups displayed weaker adhesion (5.21–6.36 MPa). The incorporation of nanoparticles enhanced bond strength across all groups without affecting the DC (75.05 % versus 76.44 %).</div></div><div><h3>Conclusion</h3><div>The Er: YAG laser is as effective as HFA for conditioning ceramic brackets, while mitigating the safety hazards associated with handling toxic acids. SepNPs enhance adhesive performance without affecting the curing process. Conversely, C-PC-PA was found to be ineffective for ceramic bracket conditioning.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"274 ","pages":"Article 113346"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145878600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scalable fabrication of polymeric dissolving microneedles for optimized ALA delivery in photodynamic therapy","authors":"Dianeth Sara Lima Bejar ,&nbsp;Michelle Barreto Requena ,&nbsp;Mirian Denise Stringasci ,&nbsp;Marlon Rodrigues Garcia ,&nbsp;Erika Toneth Ponce Ayala ,&nbsp;Juliana Cristina Barreiro ,&nbsp;Sebastião Pratavieira ,&nbsp;Vanderlei Salvador Bagnato","doi":"10.1016/j.jphotobiol.2025.113319","DOIUrl":"10.1016/j.jphotobiol.2025.113319","url":null,"abstract":"<div><div>Topical photodynamic therapy (PDT) is a minimally invasive, clinically approved treatment for non-melanoma skin cancer that relies on the conversion of photosensitizer (PS) precursors such as 5-aminolevulinic acid (ALA) into protoporphyrin IX (PpIX), followed by light activation. However, the low skin penetration of topically applied ALA cream remains a major limitation, restricting effective PpIX accumulation in deeper tumor layers. To address this challenge, we produced dissolving microneedles (DMN) as an alternative intradermal delivery platform. Two mold types were evaluated for DMN fabrication, one with a slight edge (DMNe) and another without edges (DMNf), both maintaining a conical tip geometry. DMN were prepared with a formulation containing initially 10% ALA and 20% Gantrez® AN-139 polymer in water, produced in a few steps, and characterized. <em>In vitro</em> insertion studies demonstrated consistent penetration depths of approximately <span><math><mrow><mn>250</mn><mspace></mspace><mi>μ</mi><mi>m</mi></mrow></math></span> with minimal tip deformation. DMNf showed a better penetration efficiency than the DMNe and cream groups, and mass spectrometry confirmed uniform ALA distribution. <em>In vitro</em> assays in darkness confirmed the formulation’s biocompatibility with tumor cells. In a murine xenograft model of nodular epidermoid carcinoma, DMN-mediated ALA delivery generated up to twice the amount of PpIX in deeper tumor regions and also caused greater PDT damage compared to cream application. These findings highlight DMN as a promising approach to enhance PDT efficacy, especially for thicker or nodular skin lesions, by enabling superior and uniform intradermal drug delivery.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"274 ","pages":"Article 113319"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145691428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photodamaged PSII does not accumulate in the non-appressed thylakoid membranes in the absence of PSII repair","authors":"Cleo Bagchus,&nbsp;Lennart A.I. Ramakers,&nbsp;Dana Verhoeven,&nbsp;Herbert van Amerongen,&nbsp;Emilie Wientjes","doi":"10.1016/j.jphotobiol.2025.113317","DOIUrl":"10.1016/j.jphotobiol.2025.113317","url":null,"abstract":"<div><div>Photosystem II (PSII) is highly sensitive to light-induced damage. Photoinhibition, the light-dependent inactivation of PSII, is associated with an increase of excitation-energy quenching. Recovery from photoinhibition involves migration of PSII complexes from the appressed to the non-appressed region of the thylakoid membrane, where D1 (the PSII core protein most sensitive to photodamage) is degraded and repair occurs. However, it remains unclear whether damaged PSII core complexes accumulate in the stroma lamellae when repair is blocked.</div><div>Here, we combined confocal Fluorescence Lifetime Imaging Microscopy (FLIM) with biochemical fractionation of the thylakoid membrane to investigate the localization of damaged PSII following photoinhibition in the presence of lincomycin, an inhibitor of D1 synthesis. This condition mimics natural stress scenarios such as heat, where D1 synthesis is impaired.</div><div>FLIM analysis of structured, intact thylakoid membranes, segmented into grana- and stroma-lamellae-enriched regions, revealed a decrease in PSII fluorescence lifetime upon photoinhibition, consistent with increased excitation-energy quenching. Surprisingly, no significant difference in fluorescence lifetime components was observed between membrane domains, suggesting that damaged, quenched PSII does not accumulate in the stroma lamellae under these conditions. Western blot analysis of biochemically isolated membrane fractions confirmed a uniform decrease in D1 levels across grana and stroma lamellae upon photoinhibition.</div><div>Our results indicate that when D1 synthesis is blocked, the relocation and degradation of photodamaged PSII proceed efficiently enough to prevent its accumulation in the stroma lamellae. This reveals new aspects of PSII repair and demonstrates the strength of FLIM for spatially resolved analysis of the thylakoid membrane.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"274 ","pages":"Article 113317"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145691447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cyclic electron transport pathways around photosystems I and II: Working together","authors":"Vladimir Lysenko ,&nbsp;Ya Guo ,&nbsp;Maria Ignatova ,&nbsp;Ekaterina Tarik ,&nbsp;Veronica Aslanyan ,&nbsp;Tatyana Varduny ,&nbsp;Anastasia Toptunova ,&nbsp;Pavel Plyaka ,&nbsp;Evgeniya Kirichenko ,&nbsp;Pavel Dmitriev ,&nbsp;Vladimir Krasnov","doi":"10.1016/j.jphotobiol.2025.113312","DOIUrl":"10.1016/j.jphotobiol.2025.113312","url":null,"abstract":"<div><div>The processes of cyclic electron transport around photosystems I and II (CET-PSI and CET-PSII) do not lead to O₂ evolution and CO₂ assimilation and are referred to as anoxygenic photosynthesis a broad sense, in contrast to specific processes in bacteria, which are commonly referred to as anoxygenic photosynthesis in a narrow sense. CET-PSI has been studied much more extensively than CET-PSII. Attempts to quantify CET-PSI have yielded contradictory results. It is not detected in non-stressed C3-plants using photoacoustic methods but is commonly considered as being observed when using Antimycin А which was had previously been proposed as a CET-PSI inhibitor. However, most researchers ignore recent data showing that Antimycin А primarily inhibits rather CET-PSII then CET-PSI. These facts, along with others, suggest that the contribution of CET-PSI to photosynthesis of non-stressed C3-plants has been overestimated. Our analysis of the data in this field also shows the possibility of underestimating CET-PSII, as well as anoxygenic photosynthesis in total, which is not excluded from being dominated over oxygenic photosynthesis. We point out that CET-PSI and CET-PSII cannot be studied separately. The difficulties in the quantitative evaluation of CET-PSII can be solved using photoacoustic techniques, which are highly promising in studies of anoxygenic photosynthesis.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"274 ","pages":"Article 113312"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145596759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotypic, physicochemical and transcriptomic responses of Camellia sinensis cv. ‘Huangjinya’ to UV-B stress","authors":"Yueyue Tian ,&nbsp;Meina Jin ,&nbsp;Hanyue Wang ,&nbsp;Tebin Zhang ,&nbsp;Zhengqun Zhang ,&nbsp;Ping Sun","doi":"10.1016/j.jphotobiol.2025.113313","DOIUrl":"10.1016/j.jphotobiol.2025.113313","url":null,"abstract":"<div><div>Ultraviolet-B (UV-B) is an important environmental factor that seriously affects the biological process of <em>Camellia sinensis</em> cv. ‘Huangjinya’, which is highly susceptible to light stress. To understand the mechanism adapted to UV-B, we analyzed the phenotypical, physicochemical and transcriptomic responses of ‘Huangjinya’ in response to UV-B stress in this study. Phenotypic analysis revealed that UV-B induced a yellowing of ‘Huangjinya’ leaves, accompanied by a significant reduction in chlorophyll <em>a</em>nd carotenoid content. Physiological assessments showed a marked decline in photosynthetic capacity, with decreased photosynthetic rate, stomatal conductance, and electron transfer rate, alongside increased non-photochemical quenching. Antioxidant enzyme activities, particularly peroxidase (POD), were significantly reduced, while stress-responsive hormones such as abscisic acid (ABA), jasmonic acid (JA), salicylic acid (SA), and ethylene (ET) were elevated. Under UV-B exposure, a total of 15,170 differentially expressed genes (DEGs) were identified, with 3565 upregulated and 11,605 downregulated genes. These DEGs were primarily involved in metabolic pathways related to pigment biosynthesis, photosynthesis, antioxidant enzymes, phenylpropanoid biosynthesis, and plant hormone signal transduction. Transcriptomic analysis further indicated downregulation of genes associated with chlorophyll <em>a</em>nd carotenoid biosynthesis, as well as photosynthesis-related genes, while genes involved in flavonoid biosynthesis and stress hormone pathways were upregulated. Additionally, UV-B exposure led to a decrease in free amino acids, caffeine, and soluble sugar content, but an increase in tea polyphenols. These findings suggest that UV-B enhances the ornamental value of ‘Huangjinya’ leaves but adversely affects photosynthetic efficiency and the accumulation of key quality components in tea leaves. The study provides a comprehensive understanding of the molecular and physiological mechanisms underlying response to UV-B stress of ‘Huangjinya’, highlighting the interplay between gene expression and physiological changes in UV-B adaptation.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"274 ","pages":"Article 113313"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145622354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing femtosecond laser parameters to enhance cisplatin efficacy in triple-negative breast cancer cells","authors":"Aya E. Mohamed ,&nbsp;Wafaa R. Mohamed ,&nbsp;Mai A. Elhemely ,&nbsp;El-Shaimaa A. Arafa ,&nbsp;Hany A. Omar ,&nbsp;Tarek Mohamed","doi":"10.1016/j.jphotobiol.2025.113308","DOIUrl":"10.1016/j.jphotobiol.2025.113308","url":null,"abstract":"<div><div>Triple-negative breast cancer (TNBC) is a highly aggressive malignancy with a poor prognosis due to the absence of target receptors, which limits treatment options to cytotoxic chemotherapy, particularly cisplatin. Severe adverse effects and cellular resistance limit cisplatin therapy. Laser-based therapies hold promise as adjunctive approaches with potent anti-tumor properties. This study evaluates the effects of femtosecond laser (FSL) on the MDA-MB-231 cell line alone and in combination with cisplatin. Cells were exposed to varying parameters, including wavelength (690, 750, 830, 888, and 920 nm), exposure time (10, 15, 20, and 30 min), and power settings (150, 200, 250, and 300 mW). Results revealed that 920 nm significantly reduced cell viability relative to the control. Adding cisplatin after FSL (920 nm) significantly reduced cell viability relative to cisplatin. By varying exposure time and power, 30 min of exposure significantly reduced viability relative to 15 and 20 min, and 200 mW was the most effective power compared to 250 mW and 300 mW. Trypan blue and Rhodamine 6G staining revealed that FSL + cisplatin showed a marked reduction in cell number and vesicle-like structures with condensed or absent nuclei. UV–Vis spectrophotometry showed a peak at 230 nm of intracellular cisplatin with absorbance lower in the FSL + cisplatin compared to cisplatin. These findings highlight the potential of FSL as a novel adjunctive therapy with cisplatin in TNBC treatment. By enhancing cisplatin efficacy, FSL irradiation offers a promising strategy, allowing the use of reduced doses with few adverse effects. Further studies are warranted to explore the clinical applicability of this approach.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"274 ","pages":"Article 113308"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145622353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photobiomodulation-driven cardiomyogenic differentiation and endothelial vascularization: A dual approach with Aloe Vera","authors":"Tuğçe Gültan,&nbsp;Menemşe Gümüşderelioğlu","doi":"10.1016/j.jphotobiol.2025.113331","DOIUrl":"10.1016/j.jphotobiol.2025.113331","url":null,"abstract":"<div><div>Cardiomyogenic differentiation plays a critical role in cardiac tissue engineering. It enables the transformation of precursor cells into functional cardiomyocytes capable of contraction and pumping. This study investigates the synergistic effects of <em>Aloe vera</em> (AV) and photobiomodulation (PBM) on cardiomyogenic differentiation in rat cardiomyoblast cell line derived from embryonic ventricular heart tissue (H9C2). It also examines the vascularization potential of primary rat cardiac microvascular endothelial cells (CMECs). Given their natural interaction in cardiac tissue, H9C2 and CMECs were co-cultured at a 6:1 ratio. Cytotoxicity assays established 10 mg/mL AV as a safe concentration, with cell viabilities exceeding 80 % across both monocultures and co-cultures. In the culture, PBM was applied every other day for 10 days, using a polychromatic light source (600–1200 nm) placed 20 cm away from the cells, at an irradiance of 0.04 W/cm² for 3 min per day. Neither AV nor PBM negatively affected cell viability. Fluorescence imaging indicated enhanced alignment and nuclear fusion in H9C2 cells, suggesting cardiomyogenic differentiation, particularly under combined AV–PBM treatment. Moreover, both AV and AV–PBM treatments significantly improved CMECs vascularization potential by day 14. RT-qPCR revealed a 7-fold and 9-fold increase in FGF2 gene expression following PBM and AV–PBM treatment, respectively. Anti-von Willebrand factor staining and imaging confirmed that CMECs formed lumen-like structures beneath H9C2 cells under all treatment conditions. These results demonstrate that the combined application of AV and PBM effectively promotes cardiomyogenic differentiation in H9C2 cells and enhances CMEC-mediated vascularization. This suggests a promising strategy for optimizing biosignaling and tissue integration in cardiac tissue engineering.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"274 ","pages":"Article 113331"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145742997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}