Fabrication of doxycycline-loaded mesoporous polydopamine nanoparticles for pelvic infection nursing care via synergistic photodynamic therapy

Abstract

Pelvic infection has emerged as a significant health risk to women, making the accurate identification of possible pathogenic bacteria and pathogens crucial for its prevention and treatment. Photodynamic therapy (PDT) for antibacterial purposes has emerged as an alternative and promising therapeutic approach for pelvic infections. In this investigation, we developed new antibacterial nanomaterials (DCMP NPs) using doxycycline (DCL), chitosan (CH), and mesoporous polydopamine (MPD) nanoparticles. Further, we evaluated the physicochemical properties of the DCMP NPs. TEM images indicated that fabricated DCMP NPs were spherical, with a particle size of 160.54 ± 4.56 nm. The findings showed that DCMP NPs (150 μg/mL) demonstrated favorable cytocompatibility in NIH3T3 cells and hemocompatibility, exhibiting remarkable antibacterial efficacy against Gram-negative bacteria (E. coli) and Gram-positive bacteria (S. aureus) following blue laser irradiation (1 W/cm², 450 nm). The antibacterial biofilm impact of DCMP NPs was assessed using Live/Dead staining. The laser-irradiated DCMP NPs demonstrated the most pronounced anti-biofilm impact. Overall, DCMP NPs effectively addressed nursing care for bacterial pelvic infections.