Journal of Nutritional Biochemistry最新文献

{"title":"Flavonoid Intake and Kidney Stones: an Exposome-Based Cross-Sectional Study.","authors":"Yuan Gong, Yuanyuan Yang, Lintao Miao, Mingliang Zhong, Qidong Xia, Jie Chang, Shaogang Wang","doi":"10.1016/j.jnutbio.2025.110117","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.110117","url":null,"abstract":"<p><p>The present study aimed to evaluate the relationships between total, subgroup and individual dietary flavonoid intake and the risk of kidney stones. Data from the National Health and Nutrition Examination Survey and the Food and Nutrient Database for Dietary Studies were analyzed. A total of 9033 participants without kidney stones and 985 participants with kidney stones were included. A dose-response relationship curve suggested a J-shaped relationship (p for nonlinearity <0.001) between flavanone intake and kidney stone risk (nadir point: 32.23 mg/d, OR: 0.70, 95%CI: 0.58-0.84). Naringenin (a type of flavanones) was negatively associated with kidney stone risk. Hesperetin was associated with kidney stone risk in a J-shaped manner. These findings suggest that flavanone consumption may be beneficial for individuals with elevated kidney stone risk.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"110117"},"PeriodicalIF":4.9,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145086429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the Protective Effects of Milk Osteopontin in Necrotizing Enterocolitis Neonatal Rat Model.","authors":"Lan Liu, Xuexue Li, Chenming Lin, Wei Shi, Huijia Lin, Bo Lönnerdal, Gang Yu, Zheng Chen","doi":"10.1016/j.jnutbio.2025.110108","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.110108","url":null,"abstract":"<p><p>Osteopontin (OPN) is a multifunctional milk protein with various potential health benefits. However, studies on the role of OPN during early life are still very limited. This study employed a neonatal rat model to investigate the protective effects of milk OPN against necrotizing enterocolitis (NEC), a devastating gastrointestinal disease leading high morbidity and mortality in infants. In NEC-affected rat pups, milk OPN enhanced the survival rates, and alleviated the ileum tissue damage. Immunofluorescence staining revealed that milk OPN significantly promoted intestinal cell proliferation and migration (p < 0.05). Moreover, milk OPN was found to reduced the expression of pro-inflammatory cytokines IL-6 and TNF-α in ileum tissue (p < 0.05). The activation of Toll-like receptor 4 (TLR4) and nuclear factor kappa-B (NF-κB), which are key regulators of cell proliferation and inflammation in NEC, were inhibited by milk OPN. Additionally, gut microbiota composition was also improved by milk OPN, with a reduction in the abundance of inflammation-associated bacteria, such as Proteobacteria and Klebsiella (p < 0.05), which are Gram-negative bacteria capable of activating TLR4 signaling. Our findings demonstrate the protective effects of milk OPN on NEC and propose that milk OPN may be utilized as a preventive measure against inflammatory intestinal diseases in early developmental stages.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"110108"},"PeriodicalIF":4.9,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145069803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Luteolin ameliorates skin lipid hypersecretion, inflammation and oxidative stress in vivo and in vitro.","authors":"Jie Li, Bingyong Mao, Botao Wang, Xin Tang, Qiuxiang Zhang, Tianmeng Zhang, Jianxin Zhao, Hao Zhang, Shumao Cui","doi":"10.1016/j.jnutbio.2025.110114","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.110114","url":null,"abstract":"<p><p>Luteolin (LUT) is a natural dietary ingredient with a wide range of biological functions and has been used in skin improvement. Skin lipid oversecreting is an exceedingly common disorder of the pilosebaceous glands and popular in adolescents. This study evaluated the effect of LUT on ameliorating oleic acid (OA) induced skin lipid accumulation in C57BL/6 mice and SZ95 cells. The level of major lipids and some key lipid metabolism markers were assessed, including triglyceride (TG), non-esterified fatty acid (NEFA), cholesterol, sterol regulatory-binding protein 1 and peroxisome proliferator-activated receptors. In vivo data showed decreasing significantly cholesterol levels and in vitro data demonstrated the remarkable influence in the reduction of TG and NEFA levels. Besides, LUT affected the diversity and structure of gut bacteria, an increase in the relative abundance of Akkermansia, Bacteroides and Alistipes, which were associated with the anti-oxidative and anti-inflammatory process. Additionally, our study presented that LUT significantly reduced the level of malondialdehyde (MDA, a lipid peroxidation marker), inhibited the expression of pro-inflammatory factor (TNF-α, IL-6) as well as up-regulated the mRNA expression level of SIRT1, indicating LUT alleviating inflammation and enhancing antioxidation may be associate with the activation of SIRT1 pathway.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"110114"},"PeriodicalIF":4.9,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diets enriched with extra virgin olive oil prevent alterations in the uterus of female fetuses and prepubertal offspring of diabetic rats.","authors":"Cintia Romina Gatti, Virginia Soledad Taylor, Florencia Schibert, Evangelina Capobianco, Romina Higa, Alicia Jawerbaum","doi":"10.1016/j.jnutbio.2025.110112","DOIUrl":"10.1016/j.jnutbio.2025.110112","url":null,"abstract":"<p><p>Alterations in prolactin and PPAR pathways, involved in decidualization, are programmed in the decidualized uterus of prepubertal offspring of diabetic dams. Here, we investigated whether these alterations are also present in the fetal uterus of diabetic dams and evaluated pro-oxidant/pro-inflammatory markers in the uterus of fetuses and offspring of diabetic dams. We also tested whether these parameters are regulated by maternal diets enriched with extra virgin olive oil (EVOO). Control and mild pregestational diabetic female Albino Wistar rats, mated with control males, were fed diets enriched or not with 6% EVOO during pregnancy. Fetuses were evaluated on day 21 of pregnancy, and offspring on postnatal day 30 after induction of uterine decidualization. In the fetuses of diabetic dams, the uterus showed reduced prolactin levels and reduced prolactin receptor expression, alterations that were no longer observed when the dams received the EVOO-enriched diet. Reduced PPARγ expression and increased levels of its negative regulator miR-19b were found in the fetal uteri of diabetic dams, alterations persisting when the dams received the EVOO-enriched diet. Markers of the pro-inflammatory state were not increased in the fetal uteri, but were increased in the decidualized uteri of the offspring of diabetic dams, alterations that were prevented by the maternal EVOO-enriched diet. Increased uterine lipoperoxidation markers were found both at the fetal stage and in the offspring of diabetic dams, alterations prevented by the maternal EVOO-enriched diet. Our results demonstrate a beneficial role of EVOO-enriched maternal diet in the programming of uterine impairments.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"110112"},"PeriodicalIF":4.9,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Urolithin A protects mice against osteoarthritis by inhibiting chondrocyte ferroptosis through activating AMPK/mTOR/HIF-1α signaling pathway","authors":"Xukai Wang ,&nbsp;Qinli Xu ,&nbsp;Jinzheng Huang ,&nbsp;Yating Gao ,&nbsp;Fangliang Zheng ,&nbsp;Guangyao Liu","doi":"10.1016/j.jnutbio.2025.110111","DOIUrl":"10.1016/j.jnutbio.2025.110111","url":null,"abstract":"<div><div>Urolithin A (UA) is a metabolite of natural polyphenols tannic acid and tannic acid produced by the gut microbiota that has multiple pharmacological effects. However, the effects of UA on osteoarthritis (OA) have not been reported. The aim of this study was to study the effect of UA on OA and clarify the possible mechanism. The mouse OA model was established and the mouse chondrocytes were isolated and cultured. The effect of UA on the cell viability of chondrocytes was tested by MTT assay. The levels of prostaglandin E2 (PGE2), Nitric Oxide (NO), Matrix Metalloproteinase-1 (MMP1), and Matrix Metalloproteinase-3 (MMP3) were measured by the detection kits. The expression of ferroptosis, nuclear factor-kappa B (NF-κB), and Adenosine Monophosphate-Activated Protein Kinase (AMPK) pathway related proteins were detected by western blot. The results showed that UA could attenuate the cartilage tissue injury, MMP1, and MMP3 expression in vivo. UA significantly suppressed PGE2, NO, MMP1, and MMP3 production, and NF-κB activation induced by Interleukin-1 beta (IL-1β). UA attenuated IL-1β-induced MDA, Fe²⁺, and up-regulated glutathione (GSH) production, GPX4, and ferritin expression, which suggested UA could attenuate IL-1β-induced ferroptosis. Furthermore, UA could upregulate the expression of AMPK, and downregulate the expression of mTOR and HIF-1α induced by IL-1β. In addition, AMPK inhibitor compound C prevented the inhibition of UA on IL-1β-induced PGE2, NO, MMP1, MMP3, and ferroptosis. In conclusion, the data indicated that UA exhibited therapeutic effects against OA through inhibiting inflammation and ferroptosis via the AMPK/mTOR/HIF-1α signaling pathway.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"147 ","pages":"Article 110111"},"PeriodicalIF":4.9,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145058531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of GPER-mediated AMPKα signal in the prevention effect of (-)-epicatechin on metabolic dysfunction-associated steatohepatitis.","authors":"Yulei Wang, Fuya He, Shujuan Zhao, Ping Gong, Haitian Ma","doi":"10.1016/j.jnutbio.2025.110106","DOIUrl":"10.1016/j.jnutbio.2025.110106","url":null,"abstract":"<p><p>Metabolic dysfunction-associated steatohepatitis (MASH) is one of the most prevalent liver diseases worldwide. Effective drugs and early diagnostic tools remain lacking, underscoring the urgent need for improved preventive measures, such as dietary interventions. (-)-Epicatechin (EC), a naturally polyphenolic compound that exists in tea, chocolate, and various fruits, has high bioavailability and exhibits evident lipid-lowering, antioxidant, and anti-inflammatory properties. Although the beneficial regulation effect of EC on metabolism disorders has been reported, the effects and specific molecular mechanisms by which EC mitigates the occurrences of MASH remain unclear. The present study demonstrated that incorporating EC into the diet prevented the onset and progression of MASH, which presented as the alleviation effects of EC treatment on the high-fat/high-cholesterol diet-induced liver damage, steatosis, apoptosis, oxidative stress, inflammation, and fibrosis in mice. In vitro, we also found that EC treatment effectively mitigated lipid accumulation and oxidative stress in palmitic acid-challenged hepatocytes. Mechanistically, the present study novelty certified that EC mainly up-regulates the G protein-coupled estrogen receptor (GPER) expression, a non-classical steroid receptor, mediating the activation of AMP-activated protein kinase alpha (AMPKα) signaling pathway, thereby mitigating the occurrences and progression of MASH. In summary, EC prevents the occurrences of MASH via activating the GPER-mediated AMPKα signaling pathway, which provides a substantial theoretical foundation and prompts the potential application value for EC as a candidate nutritional regulator in preventing metabolic-related diseases.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"110106"},"PeriodicalIF":4.9,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145040331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Batatasin III alleviates slow transit constipation by regulating gut microbiota and inhibiting the NLRP3-IL-1β pathway","authors":"Fangxu Yin ,&nbsp;ZiYing Jiang ,&nbsp;Yunbin Tong ,&nbsp;Song Wang,&nbsp;Kai Zheng,&nbsp;Lu Han,&nbsp;Wenyue Ma,&nbsp;Rui Li,&nbsp;Xiaohui Dou,&nbsp;Yaqian Ping,&nbsp;Bozhao Wu,&nbsp;Lang Fu,&nbsp;Daqing Sun","doi":"10.1016/j.jnutbio.2025.110110","DOIUrl":"10.1016/j.jnutbio.2025.110110","url":null,"abstract":"<div><div>Slow transit constipation (STC) is a functional gastrointestinal disorder characterized by impaired intestinal motility, inflammatory responses, and gut microbiota dysbiosis. Batatasin III, a natural stilbene compound, has been demonstrated to exhibit anti-inflammatory and neuroprotective properties; however, its role in STC remains unclear. In this study, a loperamide-induced STC mouse model was established and treated with low and high doses of Batatasin III. The therapeutic effects were evaluated through general phenotypic observation, small intestinal transit rate measurement, hematoxylin-eosin (HE) staining, enzyme-linked immunosorbent assay (ELISA), quantitative reverse transcription polymerase chain reaction (qRT-PCR), Western blotting, immunofluorescence, and 16S rRNA sequencing. Furthermore, Spearman correlation analysis was conducted to investigate the relationships between the gut microbiota and inflammatory cytokines and neurotransmitters. Batatasin III significantly improved fecal parameters and intestinal transit rate in mice, restored colonic tissue structure, and modulated levels of neurotransmitters such as 5-hydroxytryptamine (5-HT) and substance P (SP). Additionally, it inhibited activation of the NLRP3–IL-1β signaling pathway and reduced the expression of pro-inflammatory cytokines. Gut microbiota sequencing revealed an increase in beneficial bacteria such as <em>Parabacteroides</em> and <em>Faecalibaculum</em>, alongside a decreased abundance of the pro-inflammatory <em>Rikenellaceae</em>. These microbial changes were significantly correlated with both inflammatory markers and neurotransmitter levels. Batatasin III alleviates STC-related symptoms by modulating the gut microbiota and inhibiting the NLRP3–IL-1β inflammatory pathway. Its therapeutic effects may be mediated through the microbiota–gut–brain axis (MGBA), highlighting its potential value as a novel therapeutic agent for the treatment of slow transit constipation.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"147 ","pages":"Article 110110"},"PeriodicalIF":4.9,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145040412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary supplementation with host gut-derived Bacillus tequilensis (GCB-3) enhances growth, immune function, antioxidant capacity, and resistance to poly(I: C)-induced viral infection in grass carp (Ctenopharyngodon idella)","authors":"Xin Wang ,&nbsp;Bowen Li ,&nbsp;Xinyu Lei ,&nbsp;Lili Lin ,&nbsp;Linhai Yu ,&nbsp;Yueqi Wang ,&nbsp;Zhixin Guo ,&nbsp;Guiqin Wang ,&nbsp;Dongming Zhang ,&nbsp;Yu-ke Chen","doi":"10.1016/j.jnutbio.2025.110109","DOIUrl":"10.1016/j.jnutbio.2025.110109","url":null,"abstract":"<div><div>This study investigated the effects of dietary supplementation with host gut-derived <em>Bacillus tequilensis</em> (<em>GCB-3</em>) on growth performance, serum immunity, intestinal barrier function, antioxidant capacity, and resistance to poly(I: C)-induced viral infection in grass carp (<em>Ctenopharyngodon idella</em>). Fish were fed diets containing <em>GCB-3</em> at doses of 0 (B0), 1 × 10⁶ (B6), 1 × 10⁷ (B7), 1 × 10⁸ (B8), and 1 × 10⁹ (B9) CFU/g for eight weeks. The B8 group showed significant improvements in final body weight, digestive enzyme activity, and serum immune markers compared to the control (<em>P</em> &lt; .05). Intestinal barrier integrity was enhanced in the B8 group, as indicated by reduced serum levels of permeability markers (DAO, 5-HT, ET-1, <span>d</span>-LA) and upregulation of tight junction proteins and antimicrobial peptides (<em>P</em> &lt; .05). Furthermore, the B8 group exhibited elevated antioxidant enzyme activities and gene expression in the hepatopancreas (<em>P</em> &lt; .05). Following poly(I: C) challenge, the B8 group demonstrated lower serum AST, ALT, and γ-GT levels, as well as higher albumin levels, and reduced hepatopancreatic inflammation and apoptosis through the modulation of pro- and anti-inflammatory cytokines and apoptosis-related genes (<em>P</em> &lt; .05). These results indicate that dietary <em>GCB-3</em> at 1 × 10⁸ CFU/g improves growth, immunity, and intestinal health, and confers resistance to viral-like challenge in grass carp, supporting its potential as a probiotic agent in sustainable aquaculture.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"147 ","pages":"Article 110109"},"PeriodicalIF":4.9,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145040322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuroprotective effects of butyrolactone II from Aspergillus terreus via Nrf-2/SKN-1 pathway modulation in Caenorhabditis elegans","authors":"Xiliang Yang ,&nbsp;Die Hu ,&nbsp;Shiqin Zhao ,&nbsp;Jinghua Wan ,&nbsp;Long Chen ,&nbsp;Qianqian Bao ,&nbsp;Yani Zhang ,&nbsp;Qiangqiang Wang ,&nbsp;Zebo Huang","doi":"10.1016/j.jnutbio.2025.110107","DOIUrl":"10.1016/j.jnutbio.2025.110107","url":null,"abstract":"<div><div>Fourteen compounds were isolated from the fermentation broth of <em>Aspergillus terreus</em>, all of which were isolated from <em>A. terreus</em> for the first time. Among them, butyrolactone II (<em>A9</em>) had excellent DPPH radical scavenging activity, with an EC₅₀ value of 42.05 µM superior to positive control drug BHT. Further neuroprotective activity evaluation of in <em>Caenorhabditis elegans</em> CL2355 revealed that butyrolactone II could alleviate Aβ, causing chemotaxis disorder, prolonging lifespan of <em>C. elegans</em>, and reducing 5-HT sensitivity. Butyrolactone II treatment significantly elevated the chemotaxis index of genotyped nematode CL2355 by 15.06% (<em>P</em>&lt;0.05), and reduced the sensitivity of nematodes to 5-HT, decreasing the paralysis rate by 9.8% (<em>P</em>&lt;0.05). Moreover, it significantly increased median lifespan and maximum lifespan by 20% and 26% respectively. In the RNA transcriptome, Butyrolactone II caused upregulation of 277 differentially expressed genes and downregulation of 171 differentially expressed genes, inducing the entry of transcription factor SKN-1 into the nucleus and changes in its downstream genes. The annotation and enrichment of GO and KEGG pathways indicated that differentially expressed genes might be related to pathways such as metabolic detoxification, oxidative stress, and lysosomal autophagy. The qRT-PCR validation of gene expression was consistent with transcriptomics. Butyrolactone II could significantly increase the expression of mitochondrial fission factor (<em>mff-2</em>), downstream genes related to SKN-1 (<em>dod-17, gst-38</em>), heat shock protein genes (<em>hsp-17, hsp-12.6</em>), and oxidative stress related genes (<em>cyp-14A5</em>) in nematodes, while having no significant effect on the expression level of <em>gst-33</em> gene. Taken conclusion, butyrolactone II exerts neuroprotective effects by modulating the Nrf-2/SKN-1 pathway and regulating metabolic pathways, underscoring its potential as a therapeutic strategy for Alzheimer's disease and other related neurodegenerative disorders.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"147 ","pages":"Article 110107"},"PeriodicalIF":4.9,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145040409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intermittent fasting preserves the function and histological structure but induces oxidative stress in the salivary glands of male Wistar rats","authors":"Renan José Barzotti ,&nbsp;Larissa Victorino Sampaio ,&nbsp;Arieli Raymundo Vazão ,&nbsp;José Vitor Furuya de Lima ,&nbsp;Allice Santos Cruz Veras ,&nbsp;Giovana Rampazzo Teixeira ,&nbsp;Rita Cássia Menegati Dornelles ,&nbsp;Fernando Neves Nogueira ,&nbsp;Ana Claudia de Melo Stevanato Nakamune ,&nbsp;Antonio Hernandes Chaves Neto","doi":"10.1016/j.jnutbio.2025.110097","DOIUrl":"10.1016/j.jnutbio.2025.110097","url":null,"abstract":"<div><div>Studies indicate that dietary patterns influence the function and redox balance of salivary glands. This study examined the effects of intermittent fasting (IF) on the function, histological structure, and redox balance of the salivary glands. Twenty 12-week-old male Wistar rats were randomized into two groups: <em>ad libitum</em> (AL), with continuous access to water and chow, and IF, subjected to 24-h fasting on alternate days for 12 weeks. At the end, pilocarpine-induced saliva was collected to analyze flow rate and biochemical composition, while histological structure and redox balance were assessed in the parotid and submandibular glands. IF reduced final body weight and the absolute weight of the submandibular gland while increasing the relative weight of the parotid gland. Salivary flow rate and biochemical parameters, including pH, buffering capacity, amylase, total protein, and electrolyte concentration, were similar between groups. The areas of acini, ducts, and stroma, as well as total oxidant status and markers of lipid and protein oxidative damage, did not differ between groups in either salivary gland. However, IF distinctly affected the antioxidant defense mechanisms of the salivary glands, reducing total antioxidant capacity and increasing catalase (CAT) activity in the parotid glands, while in the submandibular glands, there was higher CAT activity accompanied by reduced superoxide dismutase, glutathione peroxidase, total antioxidant capacity, and reduced glutathione concentrations. Although IF preserved the function and histological structure of the salivary glands in rats, alterations in antioxidant defense indicate oxidative stress.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"147 ","pages":"Article 110097"},"PeriodicalIF":4.9,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}