Journal of Nutritional Biochemistry最新文献

{"title":"6-shogaol, a bioactive component of ginger, alleviates aging-induced ocular inflammation and ER stress in the 25-month-old mice.","authors":"Hyeyoon Eo, Jiin Park, In Gyoung Ju, Myung Sook Oh","doi":"10.1016/j.jnutbio.2025.109980","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.109980","url":null,"abstract":"<p><p>In the elderly population, age-related macular degeneration (AMD) is a major cause of visual impairment, characterized by a thinner retinal pigment epithelium and loss of photoreceptors. 6-shogaol (6S), a component of dried Zingiber officinale Roscoe, has been studied for its multiple therapeutic effects. The current study aimed to investigate the effect of 6S supplementation on AMD. 25-month-old C57BL/6 mice were orally administered with 10 mg/kg of 6S for 28 consecutive days. The thickness of the retinal layer was measured by histological analysis. mRNA expression related to fibrosis, inflammation and endoplasmic reticulum stress was measured by real-time polymerase chain reaction. As a result, 6S increased the thickness of the retinal layer and promoted postsynaptic density protein-95 expression in the outer plexiform layer of the aged mice. Moreover, 6S suppressed ocular mRNA expression related to the fibrotic process, including transforming growth factor beta, collagen type 1 alpha 1, and alpha smooth muscle actin. Furthermore, 6S reduced pro-inflammatory cytokines including tumor necrosis factor alpha, interleukin 1 beta, cyclooxygenase-2, and inducible nitric oxide synthase in the eyeballs of aged mice. Lastly, 6S inhibited ocular endoplasmic reticulum stress measured by mRNA expression of C/EBP homologous protein and spliced X-box binding protein-1 in the aged mice. Taken together, these findings suggest that 6S and dried ginger could be a potential nutraceutical candidate for AMD or other age-related eye diseases.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"109980"},"PeriodicalIF":4.8,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Benzyl isothiocyanate ameliorates hepatic insulin resistance in mice with high-fat diet-induced nonalcoholic fatty liver disease.","authors":"Chia-Wen Lo, Jyun-Lin Lee, Wei-Ting Tsai, Chin-Shiu Huang, Ya-Chen Yang, Chong-Kuei Lii, Haw-Wen Chen","doi":"10.1016/j.jnutbio.2025.109981","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.109981","url":null,"abstract":"<p><p>The global prevalence of overweight and obesity has risen sharply over the past few decades as a result of excess calorie intake and sedentary lifestyles. Obesity increases the risk for various metabolic disorders, such as hyperlipidemia, fatty liver disease, and diabetes mellitus. Isothiocyanates, which are abundant in cruciferous vegetables, have been shown to exhibit anti-cancer, anti-inflammatory, and antioxidant properties. However, the efficacy of benzyl isothiocyanate (BITC) in preventing the adverse effects of obesity, such as hepatic steatosis and insulin resistance, remains uncertain. To address this knowledge gap, we assessed whether BITC protects against hepatic insulin resistance by using primary mouse hepatocytes and AML12 cells treated with palmitic acid (PA) and mice fed a high-fat diet supplemented with cholesterol and cholic acid (HFCCD). We found that the impairments in insulin sensitivity caused by PA, such as decreases in the phosphorylation of insulin receptor substrate (IRS) 1 (Tyr608), Akt, glycogen synthase kinase (GSK) 3β, and FOXO1 and increases in the expression of glucose-6-phosphatase (G6Pase) and phosphoenolpyruvate carboxykinase 1 (PEPCK) mRNA in hepatocytes, were mitigated by pretreatment with BITC. BITC also attenuated PA-induced hepatic lipid accumulation and reactive oxygen species production. In vivo, BITC significantly reduced blood glucose levels and the HOMA-IR and inhibited hepatic lipid accumulation, IRS1 phosphorylation at Ser307, and G6Pase and PEPCK expression compared with that in mice fed the HFCCD alone. These results show that BITC ameliorates the lipotoxicity associated with insulin resistance by activating the IR/IRS/Akt/FOXO1 and GSK3β pathways, which leads to decreased gluconeogenesis and increased glycogen synthesis.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"109981"},"PeriodicalIF":4.8,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Something more about the role of flavonoids in aging","authors":"Salvatore Chirumbolo","doi":"10.1016/j.jnutbio.2025.109925","DOIUrl":"10.1016/j.jnutbio.2025.109925","url":null,"abstract":"","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"142 ","pages":"Article 109925"},"PeriodicalIF":4.8,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144154771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blood Methylation Signatures in Childhood Obesity and Risk of Cardiac Hypertrophy in Young Adults:Findings from the BCAMS Study and Mendelian Randomization Analysis.","authors":"Xinghao Yi, Haoxue Zhu, Ling Zhong, Mengyu He, Shan Gao, Ming Li","doi":"10.1016/j.jnutbio.2025.109979","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.109979","url":null,"abstract":"<p><strong>Background: </strong>Childhood obesity may induce epigenetic changes, affecting long-term cardiac health. However, empirical evidence remains scarce. Leveraging the prospective cohort of Beijing Child and Adolescent Metabolic Syndrome study (BCAMS), we investigated the blood DNA methylation signatures associated with childhood obesity and the future risk of cardiac hypertrophy in young adults, confirming causality with Mendelian randomization (MR).</p><p><strong>Methods: </strong>The BCAMS study followed children into adulthood. Data including blood DNA methylation profiles, along with lifestyles, blood levels of 7 adipokines and 32 amino acids were collected at baseline. Echocardiographic measurements were assessed at the 10-year follow-up. Enrichment and correlation analyses were performed, and two-sample MR analyses involving 105,268 participants from European biobanks were employed to infer causality.</p><p><strong>Results: </strong>Cross-sectionally, we identified 376 differentially methylated sites between children with obesity and normal-weight controls (n=25), particularly within genes enriched in the cardiomyopathy pathway. Longitudinally, 11 childhood obesity-associated methylation sites, especially cg25835058 (KAZN), cg01362389 (TDH) and cg12099423 (SLC17A9), showed strong correlations with left ventricular index (LVMI) at the 10-year follow-up (p <0.0017). Additionally, these sites were associated with traditional risk factors, notably glutamine, which displayed strongest protective association with LVMI (-1.72g/m^2.7 per 1SD increase, p <0.001) when validated with the entire cohort (n=326). MR analysis confirmed the significant correlation between cg12099423 methylation levels and SLC17A9 expression, and the causality between gene expression levels (KAZN, TDH, SLC17A9) and LVMI.</p><p><strong>Conclusions: </strong>Methylation associated with childhood obesity, particularly SLC17A9, may function as an epigenetic mechanism impacting long-term cardiac health later in life, emphasizing the significance of early intervention.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"109979"},"PeriodicalIF":4.8,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbiome, a potential modulator of neuroepigenome.","authors":"Ruili Yang, Yi Xu, Feng Zhu, Xiaojing Ma, Tingting Fan, Hui-Li Wang","doi":"10.1016/j.jnutbio.2025.109961","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.109961","url":null,"abstract":"<p><p>Gut microbiome has a considerable impact on the central nervous system via the \"gut-brain axis\". Neuroepigenome emerges as the interface between environment and genes, potentially help conveying the signals derived from the microbiome to the brain tissue. While only a limited number of studies have implicated epigenetic roles in the gut-brain axis, this review explores how gut microbiome might impact various brain-based epigenetic mechanisms, including DNA methylation, histone modification, ncRNA and RNA methylation, notably in the context of the specific neural complications. Among the epigenetic mechanisms, histone acetylation was most well-studied with respect to its relationships with gut microbiome, exerting a dynamic influence on gene expression in the brain. Furthermore, the pathways connecting gut bacteria to neuroepigenome were summarized, highlighting the roles of metabolites such as butyrate, propionate, acetate, lactate, and folate. Of particular interest, the roles of butyrate are emphasized due to their outstanding inhibitory activity towards histone deacetylases (HDACs), among other mechanisms. It is worth noting that some indirect gut-brain pathways may also be associated with the interplay between microbiome and neuroepigenome, while IL-6 has been found to effectively transmit microbe-derived signals to histone methylation in brains. Finally, we recapitulate the future perspectives critical to understanding this gut-brain crosstalk, such as clarifying the cause-and-effect relationship, bacterial cross-feeding within the gut, and the mechanisms underlying the site-specific histone modification in the brain. Together, this review attempts to consolidate our current knowledge about the \"microbiome-neuroepigenome interplay\" and propose a conceptual pathway to decipher the gut-brain axis in various neurological conditions.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"109961"},"PeriodicalIF":4.8,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary addition of fraxetin improved intestinal structure and growth performance in juvenile grass carp (Ctenopharyngodon idella): as a potential novel phytogenic feed additive.","authors":"Jie Zhang, Wei-Dan Jiang, Pei Wu, Yang Liu, Yao-Bin Ma, He-Qun Shi, Sheng-Yao Kuang, Shu-Wei Li, Ling Tang, Xiao-Qiu Zhou, Lin Feng","doi":"10.1016/j.jnutbio.2025.109969","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.109969","url":null,"abstract":"<p><p>The well-being and development of fish are affected to varying degrees under the intensive aquaculture model, and the use of Chinese herbs for aquaculture disease control and feed additives has received increasing attention. This study examined fraxetin supplementation in juvenile grass carp to investigate its effects on growth and intestinal structure. There were 1080 grass carp (11.58 ± 0.01 g) assigned to 6 treatments, fed with fraxetin (0, 3.9, 7.9, 15.8, 31.5, and 63.1 mg/kg) for 60 days in each treatment. In our study, appropriate fraxetin significantly increased final body weight (FBW), percent weight gain (PWG), and specific growth rate (SGR) compared to the unadded group (P < 0.05), but did not affect feed efficiency (FE) (P > 0.05). The administration of 7.9 mg/kg of fraxetin significantly improved fish intestinal development and body composition. Appropriate dietary fraxetin significantly enhanced intestinal digestive enzymes and brush border enzyme activity (P < 0.05), decreased serum diamine oxidase (DAO) levels (P < 0.05), and decreased intestinal cell apoptosis (P < 0.05). Appropriate levels of fraxetin inhibited the RhoA/ROCK signaling pathway while upregulating both mRNA and protein expression of tight junction (TJ) and adherens junction (AJ) (P < 0.05). These changes significantly improved apical junction complex (AJC) integrity. In conclusion, dietary supplementation with appropriate levels of fraxetin added to the diets had a facilitating effect on digestion and absorption, improved intestinal structure, and promoted fish growth performance in juvenile grass carp. In addition, the optimal dietary fraxetin levels were evaluated to be 6.06 and 7.79 mg/kg based on linear regression analysis of PWG and DAO, respectively.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"109969"},"PeriodicalIF":4.8,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of fish oil supplement on brain lipids profile in ApoE-/- mice and C57BL/6J mice treated with diets containing different dosage of vitamin E.","authors":"Lu Liu, Xiaochen Huang, Jingjing Xu, Xixiang Wang, Yu Liu, Xiuwen Ren, Chi Zhang, Ying Wang, Yueyong Wang, Shaobo Zhou, Linhong Yuan","doi":"10.1016/j.jnutbio.2025.109964","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.109964","url":null,"abstract":"<p><p>The study was designed to explore the impact of fish oil supplement on the brain lipids profile in ApoE-deficient (ApoE-/-) mice and wild-type C57BL/6J (C57 wt) mice treated with diets containing varying dosage of vitamin E (VE). Compared to C57 wt mice, ApoE-/- mice exhibited increased cortical TC and LDL-C levels, but decreased HDL-C level (P < 0.05). The fish oil intervention, combined with low dietary VE intake resulted in increased cortical protein expression of CD36 and PPARγ in C57 wt mice. PI (36:5) was identified as a significantly different metabolite when compared to the control group in both C57 wt and ApoE-/- mice. The combination of fish oil and varying doses of VE had different effects on the expression of brain lipids and fatty acid transporters in ApoE-/- and C57 wt mice, indicating that ApoE status and dietary VE intake influence the responses of the cerebral lipids profile to fish oil supplementation.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"109964"},"PeriodicalIF":4.8,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"n-3 polyunsaturated fatty acids-enriched fish oil attenuates chronic alcohol-induced liver injury via a mechanism involving the upregulation of Retsat.","authors":"Yuxuan Zhang, Kaixin Pan, Angcheng Xu, Shuzhen Sun, Qingling Huang, Yicheng Wang, Hao Wang, Qiang Han, Duo Li, Qinchao Ding, Jiaomei Li","doi":"10.1016/j.jnutbio.2025.109971","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.109971","url":null,"abstract":"<p><p>This study aimed to delineate the protective role of fish oil against alcoholic liver disease (ALD), identify the principal active component between eicosapentaenoic acid (EPA, C20:5 n-3) and docosahexaenoic acid (DHA, C22:6 n-3), and elucidate the molecular mechanisms. C57BL/6J mice were randomly assigned to receive either an alcohol-fed (AF) or pair-fed control (PF) diet, enriched with fish oil (FO) or corn oil (CO) for four weeks. Additionally, a series of in vitro experiments were performed using AML-12 cells to further investigate potential mechanisms. The results showed that plasma levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were significantly lower in the AF-FO group compared to the AF-CO group, indicating that fish oil alleviated alcohol-induced liver damage. Hepatic antioxidant markers, including glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT) were also higher in the AF-FO group than in the AF-CO group. Transcriptomic analysis revealed FO supplementation significantly affected genes involved in oxidoreductase activity and lipid metabolism pathways, with Retsat being the most up-regulated gene. The in vitro experiments indicated that DHA, but not EPA, markedly increased Retsat expression, cell viability, and the expression of genes related to oxidoreductase activity and lipid metabolism, compared to linoleic acid (LA, C18:2 n-6). Notably, knocking down Retsat abolished the protective effects of DHA. In conclusion, dietary fish oil mitigated chronic alcohol-induced liver injury primarily through DHA by upregulating Retsat and downstream genes associated with oxidoreductase function and lipid metabolism.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"109971"},"PeriodicalIF":4.8,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"1,25(OH)₂D₃ protected against LPS-induced acute lung injury through modulation of gut microbiota.","authors":"Xiaoming Zhang, Ye Li, Yiting Gong, Ning Jia, Yuanqing Luo, Jianrong Cao, Yan Yuan, Zitian Wang, Xueyuan Zhi","doi":"10.1016/j.jnutbio.2025.109970","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.109970","url":null,"abstract":"<p><p>This study investigated whether gut and lung microbiota mediated the effects of 1,25-dihydroxyvitamin D₃ [1,25(OH)₂D₃] against acute lung injury (ALI). We evaluated the impact of various 1,25(OH)₂D₃ doses on lipopolysaccharide (LPS)-induced ALI and characterized microbial changes in both intestinal and pulmonary communities. Correlation between lung and gut microbiota was assessed. To further explore the role of intestinal flora, a depletion model was established using antibiotics (ABX). Our results indicated that 1,25(OH)₂D₃ alleviated LPS-induced ALI, as evidenced by reduced pathological damage, downregulated expression of pro-inflammatory cytokines (IL-1β, IL-6, TNF-α), increased levels of anti-inflammatory cytokine (IL-10), and suppressed TLR4/NF-κB and JNK pathways. LPS reduced α diversity and altered β diversity and composition of intestinal microbes, which were partially reversed by 1,25(OH)₂D₃ intervention. Notably, 1,25(OH)₂D₃ enhanced gut microbiota diversity and elevated the relative abundance of Muribaculaceae and Lachnospiraceae genus. Importantly, depletion of gut flora with ABX eliminated the anti-inflammatory effects of 1,25(OH)₂D₃, including its inhibition of LPS-induced cytokine expressions and pathway activation. Although LPS did not significantly affect the diversity of lung microbiota, it seemed to change its composition and induced a significant correlation between intestinal and pulmonary microbial communities, which was attenuated by 1,25(OH)₂D₃. In conclusion, our findings suggested that the protective effects of 1,25(OH)₂D₃ against LPS-induced ALI might be partially mediated by gut microbiota, highlighting a potential mechanism for vitamin D's immunomodulatory activity in inflammatory lung disease.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"109970"},"PeriodicalIF":4.8,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intake of Neutral core Human Milk Oligosaccharides (HMOs) during early life improves long-term bone quality.","authors":"N Bonnet, F N Schmidt, M Baruchet, E Migliavacca, M Ramos-Nieves, L Favre, D Brassart, K Mletzko, B Busse, Koopmans Sietse-Jan, M N Horcajada","doi":"10.1016/j.jnutbio.2025.109968","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.109968","url":null,"abstract":"<p><p>Infancy is a key period for bone growth, determining bone strength later in life. Studies have provided early insights into the role that human milk oligosaccharides (HMOs) may play in growth, potentially through their effects on the microbiome. However, focus was on sialylated HMOs whereas neutral core HMOs represent the most abundant class in human breast milk. We explored impact on bone quantity, quality and strength in 1year old female minipigs exposed to neutral HMOs during preweaning. Milk formula led to significant lower bone mineral density and quality compared to sow-fed (positive control). Milk formula enriched in neutral HMOs led to higher tibia bone density, structure and matrix quality compared to milk formula, leading to similar bone strength as in naturally sow-fed, whereas sialylated HMOs was not able to significantly differentiate than milk formulae. We identify a specific correlation between neutral HMOs, bone and microbiome, with the novel taxonomies Turicibacter sanguinis and Paraprevotella clara associating with HMO-mediated bone strength. This study provides important insights on the role of different HMO subtypes on bone health and possible associated adaptations in microbiome taxa, providing perspectives for new nutritional solutions for bone growth in early life.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"109968"},"PeriodicalIF":4.8,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}