Journal of Nutritional Biochemistry最新文献

{"title":"The fermentation of whey protein and mulberry polyphenols by forming protein-phenolic adducts: Improved digestions","authors":"Qi Yuan ,&nbsp;Huaigu Yang ,&nbsp;Jingrong Cheng ,&nbsp;Xueming Liu","doi":"10.1016/j.jnutbio.2025.109921","DOIUrl":"10.1016/j.jnutbio.2025.109921","url":null,"abstract":"<div><div>The impacts of forming adduct between whey protein (WP) and mulberry polyphenol (MP) on the digestion and fermentation of WP and MP were investigated using an <em>in vitro</em> model. The results showed that MP increased the <em>in vitro</em> antioxidant capacity of WP digestive products. After forming adduct the total extractable phenolic content of MP dropped from 440.20 mg GAE/g to 21.53 mg GAE/g. The total extractable phenolic content of WP-MP group decreased from 21.53 mg GAE/g to 11.77 mg GAE/g after the oral digestion, then slightly increased to 12.43 after the gastric digestion and continuously increased to 20.43 mg GAE/g after the intestinal digestion. Extractable individual phenolic compounts exhibited the similar tendency, in which cyanidin-3-<em>O</em>-glucoside, cyanidin-3-<em>O</em>-rutinoside, <em>p</em>-coumaric acid, quercetin and kaempferol were still detectable while protocatechuic and neochlorogenic acid increased after intestinal digestion of WP-MP adduct. Incorporation of MP inhibited the oral and gastric digestion but enhanced the intestinal digestion of WP, and the degree of hydrolysis of WP increased 9.70% after intestinal digestion compared to the control. The fermentation of non-dialyzable residue of WP-MP by gut flora decreased the pH value from 7.18 to 4.82 and increased the proliferation of beneficial bacteria and the production of short-chain fatty acids. These findings indicated that WP-MP adduct increased the digestion of WP and the bioaccessibility of MP, could improve the intestinal health and could be used as a new healthy food ingredient.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"142 ","pages":"Article 109921"},"PeriodicalIF":4.8,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143917969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cardioprotective effect of senotherapy in chronically obese middle-aged female rats may be mediated by a MERCSs/Nrf2 interaction","authors":"Alejandro Silva-Palacios ,&nbsp;Alejandra María Zúñiga-Muñoz ,&nbsp;Elizabeth Soria-Castro ,&nbsp;Edith Álvarez-León ,&nbsp;Mario Nieto ,&nbsp;Gabriela Navarrete-Anastasio ,&nbsp;Roxana Carbó ,&nbsp;Wylly Ramsés García-Niño ,&nbsp;Stefanie Paola López-Cervantes ,&nbsp;Verónica Salas-Venegas ,&nbsp;Rosa Pamela Flores-Torres ,&nbsp;Armando Luna-López ,&nbsp;Cecilia Zazueta ,&nbsp;Mina Königsberg","doi":"10.1016/j.jnutbio.2025.109923","DOIUrl":"10.1016/j.jnutbio.2025.109923","url":null,"abstract":"<div><div>Hypercaloric intake promotes the development of obesity, a risk factor for cardiovascular disease (CVD). In recent years, it has been suggested that senescent cells have negative implications for the outcome of these chronic pathologies, and senotherapy has emerged as a novel intervention to reduce damage to the organism. However, it is unclear whether the accumulation of senescent cells induces alterations at the cardiac level in rats fed a hypercaloric diet (HD) and if the use of senotherapeutics can reverse it. To address this question, we used middle-aged female rats fed HD from 21 days to 15 months of age. Under our experimental conditions, rats exhibited cardiac hypertrophy and fibrosis, accumulation of senescent cells, changes in mitochondrial morphology, and oxidative stress. Rats were treated for 2 months with senolytic (dasatinib + quercetin, DQ) or senomorphic (sulforaphane, SFN) agents. Interestingly, the HD rats showed cardiac improvement after the treatment. Our data suggest a possible link mechanism between Nrf2 activation and <u>m</u>itochondria-<u>e</u>ndoplasmic <u>r</u>eticulum <u>c</u>ontact <u>s</u>ites (MERCSs) preservation, activated by SFN rather than by the DQ combination, which allowed cardiac structure maintenance in HD rats decreasing the harmful effects of senescent cells.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"142 ","pages":"Article 109923"},"PeriodicalIF":4.8,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143917970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Astaxanthin obtained from freshwater crustaceans mitigates visceral adiposity by modulating adipose tissue lipogenesis and ameliorates dyslipidemia in high-sucrose diet fed rats","authors":"Matias Rodrigo Vargas ,&nbsp;María del Rosario Ferreira ,&nbsp;Pablo Collins ,&nbsp;María Eugenia D’Alessandro","doi":"10.1016/j.jnutbio.2025.109924","DOIUrl":"10.1016/j.jnutbio.2025.109924","url":null,"abstract":"<div><div>The incidence of overweight and obesity continues to grow at alarming rates around the world. The search for foods with potential benefits in the prevention/treatment of overweight/obesity and related disorders has great relevance. The aim of this study was to evaluate the effect of astaxanthin (ASTX) from freshwater crustaceans (crabs) upon visceral adiposity, adipose tissue lipid metabolism disorders and dyslipidemia present in a Metabolic Syndrome rodent model. Male Wistar rats were fed for 90 days with 1 of 4 experimental diets: a-Reference group (RD) received a standard commercial rodent diet, b- High-sucrose diet (HSD) group received a HSD, c- RD+ASTX group received a standard commercial rodent diet plus ASTX, <span>d</span>- HSD+ASTX group received a HSD plus ASTX. The rats were given orally either ASTX (10 mg/kg body weight/day in sunflower oil) or only sunflower oil. Compared with HSD-fed rats, HSD+ASTX group had lower body weight gain (19%) and both reduced abdominal circumference (5%) and visceral adiposity index (5%). Energy intake was 24% lower at the middle of the experimental period. Epididymal adipocytes size and triglyceride (TG) content was reduced by 14%. Besides, fatty acid synthase, malic enzyme and glucose-6-phosphate dehydrogenase activities in epididymal adipose tissue were 43%, 28% and 38% lower respectively. These changes were accompanied by lower TG (25%) and cholesterol (27%) serum levels, atherogenic index (31%) and reduced Systolic (12%) and Diastolic (15%) blood pressure. The results show that ASTX could be a potential strategy to prevent/attenuate the incidence of metabolic risk factors such as overweight/adiposity and dyslipidemia.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"142 ","pages":"Article 109924"},"PeriodicalIF":4.8,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"β-Hydroxy-β-methylbutyrate (HMB) increases muscle mass and diminishes weight gain in high-fat-fed mice","authors":"Meytal Cohen-Or,&nbsp;Nava Chapnik,&nbsp;Oren Froy","doi":"10.1016/j.jnutbio.2025.109926","DOIUrl":"10.1016/j.jnutbio.2025.109926","url":null,"abstract":"<div><div>β-Hydroxy-β-methylbutyrate (HMB) is a catabolite of leucine, which promotes muscle growth. However, little is known about the effect of HMB on body composition in the context of a high-fat diet. Our aim was to study the circadian metabolic effect of HMB on body weight. C57BL male mice were fed <em>ad libitum</em> chow diet (C), chow diet supplemented with 500 mg Ca-HMB per 1 kg body weight (<em>C</em>+HMB), a high-fat diet (HFD) or HFD supplemented with 500 mg Ca-HMB per 1 kg body weight (HFD+HMB) for 7 weeks. HMB led to reduced fat weight (30%, <em>P</em>&lt;.05) and body weight (7%, <em>P</em>&lt;.05) and increased muscle weight (17%, <em>P</em>&lt;.05) in the HFD+HMB group. HMB increased glucose oxidation (27%, <em>P</em>&lt;.0001) and reduced fatty acid oxidation (30%, <em>P</em>&lt;.0001) in the C group, but increased fatty acid oxidation in the HFD+HMB group (10%, <em>P</em>&lt;.05). At the molecular level, HMB did not affect metabolic proteins in the liver, but lowered NF-κB levels in adipose tissue (24%, <em>P</em>&lt;.05). In the muscle, HMB showed no activation of AKT and mTOR, but did show activation of P70S6K (67%, <em>P</em>&lt;.01) and S6 (42%, <em>P</em>&lt;.01). The activation of the P70S6K and S6 was independent of AKT and mTOR and was accompanied by increased activation of phospholipase D2 (PLD) (35%, <em>P</em>&lt;.0001). In addition, HMB led to altered circadian rhythms. In conclusion, mice fed a HFD supplemented with HMB have increased muscle weight and reduced fat mass and body weight presumably due to increased locomotor activity. HMB induces myogenesis by activating P70S6K and S6 <em>via</em> PLD2.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"142 ","pages":"Article 109926"},"PeriodicalIF":4.8,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143917972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary and lifestyle determinants of vitamin D status in the UK Biobank Cohort study for predictive modeling","authors":"Ángela Alcalá-Santiago ,&nbsp;Belén García-Villanova ,&nbsp;María Dolores Ruíz-López ,&nbsp;Ángel Gil ,&nbsp;Miguel Rodriguez-Barranco ,&nbsp;Maria José Sánchez ,&nbsp;Esther Molina-Montes","doi":"10.1016/j.jnutbio.2025.109919","DOIUrl":"10.1016/j.jnutbio.2025.109919","url":null,"abstract":"<div><div>Vitamin D (VD) is involved in a wide variety of physiological processes. The high prevalence of VD deficiency in the population requires stronger preventive measures. The aim was to characterize the dietary and lifestyle determinants of VD levels in blood and of VD deficiency to further develop predictive models of these two outcomes. A total of 63,759 participants from the UK Biobank study with available data on dietary intake of VD, assessed via 24-hour recalls, and with measurements of serum 25(OH)D levels were included. Linear and logistic regression models were applied to identify factors associated with VD levels and VD deficiency outcomes, and to evaluate the influence of covariates on the association between VD in serum and VD in the diet. Predictive models for both VD outcomes were constructed using classical regression models and machine learning methods based on penalized likelihood methods. Approximately 10% of the participants had VD deficiency (VD &lt; 25 nmol/L), and 38.9% were at risk of VD inadequacy (VD 25–49 nmol/L). The dietary intake of VD was significantly lower in the VD deficient group. This latter group showed lower engagement in physical activity (22.1%) compared to the non-deficient group (13.4%; <em>P</em>&lt;.001). Also, overweight and obesity (vs normal weight) were related to a greater likelihood of VD deficiency (OR=1.18 and 1.96, respectively). A similar odds of VD deficiency was observed for abdominal obesity (OR=1.83). A weaker association was observed between dietary VD intake, based on participant reports, and VD levels. With regard to sunlight exposure, darker skin tones (OR dark vs fair skin=3.11), season (OR winter vs autumn=3.76) and less outdoor time activities (OR per 1 h increase=0.96) were also related to VD deficiency. Predictive models for both classical regression and machine learning, showed good accuracy (AUC=0.8–0.9 for VD deficiency). In conclusion, while a rich diet in VD boosts its levels, sun exposure plays a more significant role particularly in populations from the UK or Northern Europe. A predictive model including key determinants could effectively assess VD deficiency.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"142 ","pages":"Article 109919"},"PeriodicalIF":4.8,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143917971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation of serum trace elements with clinical features and gut microbiota in patients with Crohn's disease","authors":"Yuanyuan Wang ,&nbsp;Yumei Lin ,&nbsp;Jiaxing Feng ,&nbsp;Liqun Lin ,&nbsp;Lupeng Liu ,&nbsp;Jingling Su ,&nbsp;Chenxi Xie ,&nbsp;Huaxiu Shi","doi":"10.1016/j.jnutbio.2025.109917","DOIUrl":"10.1016/j.jnutbio.2025.109917","url":null,"abstract":"<div><div>Changes of several trace elements have been reported to contribute to the pathogenesis of Crohn’s disease (CD), which is associated with gut microbiota imbalance. This study aimed to investigate changes in trace elements in Chinese CD patients, and explore the correlation of trace element status with clinical features and gut microbiota. Eighty CD patients and 45 healthy volunteers were enrolled between July 2022 and November 2022. Serum zinc, copper, magnesium and selenium were measured by inductively coupled plasma mass spectrometry. The nutritional status was assessed based on body mass index and albumin and disease severity was determined according to the Crohn's disease activity index and C-reactive protein. Fecal gut microbiota was analyzed using 16SrRNA gene sequencing. Compared with healthy controls, serum copper increased, but serum selenium reduced in Chinese CD patients. The serum levels of selenium and magnesium were positively related to nutritional status, and the serum levels of selenium and copper were associated with disease severity. Selenium deficiency in CD patients was closely related to the diversity and abundance of gut microbiota. The serum levels of several trace elements change in the CD patients and are associated with nutritional status and disease severity. Selenium deficiency in CD patients is associated with the diversity of gut microbiota, suggesting an interaction between trace elements and gut microbiota.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"142 ","pages":"Article 109917"},"PeriodicalIF":4.8,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143795219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ketone bodies in renal function and diabetic kidney disease","authors":"Hamza Mechchate","doi":"10.1016/j.jnutbio.2025.109915","DOIUrl":"10.1016/j.jnutbio.2025.109915","url":null,"abstract":"<div><div>Diabetes, as a leading cause of chronic kidney disease (CKD) and diabetic kidney disease (DKD), underscores a significant concern, especially due to its association with health decline and mortality. In this context, the roles of ketone bodies, especially beta-hydroxybutyrate are increasingly recognized for their impact in renal physiology and the pathology of DKD. Moving beyond their conventional perception as metabolic by products, ketone bodies have been found to play a crucial role in renal health, particularly under the stresses of diabetic conditions. Serving as alternative energy sources during periods of glucose scarcity, they also function as important signaling molecules. These ketones significantly influence oxidative stress, nutrient-sensing pathways, and mitochondrial function within the kidneys. The adaptability of renal cells to utilize ketone bodies in diabetes highlights a dynamic metabolic interplay, essential for understanding renal health. The exploration of ketone body metabolism modulation, particularly through interventions like SGLT2 inhibitors and ketogenic diets, opens new avenues in managing DKD. Such insights pave the way for rethinking the role of ketone bodies in renal pathology and diabetes, pointing to novel research directions and therapeutic potentials.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"142 ","pages":"Article 109915"},"PeriodicalIF":4.8,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of folic acid deficiency on ischemic stroke: Role of inflammation and long noncoding RNA H19","authors":"Meng Wang ,&nbsp;Linran Shi ,&nbsp;Zonghang Tong ,&nbsp;Yinyue Liu ,&nbsp;Yuxuan Bai ,&nbsp;Xueli Yang ,&nbsp;Yanhong Wang ,&nbsp;Zhongying Gong ,&nbsp;Qiang Zhang ,&nbsp;Xumei Zhang","doi":"10.1016/j.jnutbio.2025.109916","DOIUrl":"10.1016/j.jnutbio.2025.109916","url":null,"abstract":"<div><div>It has been validated that folic acid deficiency (FD) is associated with an increased risk of stroke and a worse prognosis. However, the specific mechanisms by which FD exerts its detrimental effects on ischemic stroke (IS) have not been fully understood. The results of this case-control study indicated that patients with IS had a decreased serum folate level, along with up-regulated long non-coding RNA H19 (lncRNA H19) and enhanced inflammatory responses. Meanwhile, it was corroborated that the serum folate level was negatively correlated with H19 expression and the systemic immune-inflammation index (SII). Similarly, FD was demonstrated to exacerbate neurological injury in the middle cerebral artery occlusion/reperfusion (MCAO/R) rats by up-regulating the expression of inflammatory cytokines and H19 in both peripheral blood and brain tissue. Notably, the alterations in the expression of these factors in peripheral blood were consistent with those observed in brain tissue. Additionally, in a co-culture of N2a neurons and BV2 microglia, FD promoted the transition of BV2 cells towards a pro-inflammatory state by up-regulating the expression of H19, which aggravated neuronal injury. Moreover, blocking H19 in BV2 cells mitigated inflammation and partially reversed the injury in N2a cells exacerbated by FD after the treatment with oxygen-glucose deprivation and reperfusion (OGD/R). These findings provide a more in-depth insight into the regulatory role of H19-mediated systemic inflammatory responses in the context of FD, suggesting the potential clinical utility of folic acid in managing ischemic brain injury.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"142 ","pages":"Article 109916"},"PeriodicalIF":4.8,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Linoleic acid modulates vascular reactivity, alters cyclooxygenase-2-derived products, and promotes eutrophic remodeling in small mesenteric arteries of normotensive rats","authors":"Dieli Oliveira Nunes,&nbsp;Vinicius Bermond Marques,&nbsp;Camila Cruz Pereira Almenara,&nbsp;Wena Dantas Marcarini,&nbsp;Eduardo Hertel Ribeiro,&nbsp;Ivanita Stefanon,&nbsp;Alessandra Simão Padilha","doi":"10.1016/j.jnutbio.2025.109913","DOIUrl":"10.1016/j.jnutbio.2025.109913","url":null,"abstract":"<div><div>Linoleic acid (LA) is a polyunsaturated fatty acid with essential roles in cardiovascular regulation. Despite its known vascular effects, its impact on mesenteric resistance arteries (MRA) in normotensive models remains unclear. This study aimed to determine whether LA induces functional and structural changes in MRA of normotensive rats by modulating endothelial mechanisms. We hypothesized that LA treatment reduces vasoconstrictor reactivity and promotes vascular remodeling via enhanced nitric oxide (NO) bioavailability. Male Wistar rats were treated with LA (15 mg/kg) or vehicle for 15 days. LA did not alter blood pressure, mechanical parameters, or collagen and elastin levels in the MRA, but it increased both external and internal diameters of the vessels, reducing the wall-to-lumen ratio. LA decreased the contractile response to phenylephrine, without affecting responses to acetylcholine or sodium nitroprusside. <span>l</span>-NAME (100 µM) enhanced vasoconstriction to a greater extent in the LA-treated group, associated with increased nitric oxide (NO) bioavailability, independent of inducible NO synthase (iNOS) and phosphatidylinositol 3- kinase (PI3K). The NADPH oxidase pathway had a lesser impact on vasoconstriction in the LA group, although the superoxide anion scavenger tiron (1 mM) and the production of reactive oxygen species showed similar results. Inhibition of ciclooxigenase (COX) and cytochrome-P450 (CYP450) attenuated vascular reactivity in the LA group, with greater involvement of COX-2-derived products. While SC 19220 (EP1 receptor antagonist, 10 µM) reduced vasoconstrictor responses to phenylephrine only in MRA from the LA-treated group, SQ 29.548 (TP receptor antagonist, 1 µM) reduced responses only in controls. In conclusion, LA reduces vasoconstriction, increases NO bioavailability and decreases NADPH oxidase participation associated with alterations in CYP450 and COX-2-derived products.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"141 ","pages":"Article 109913"},"PeriodicalIF":4.8,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Medium-chain triglyceride attenuates obesity by activating brown adipose tissue via upregulating the AMPK signaling pathway","authors":"Min Jia ,&nbsp;Hao Yue ,&nbsp;Xiuxiu Wang ,&nbsp;Aizhen Zong ,&nbsp;Tongcheng Xu ,&nbsp;Yong-Jiang Xu ,&nbsp;Yuanfa Liu","doi":"10.1016/j.jnutbio.2025.109914","DOIUrl":"10.1016/j.jnutbio.2025.109914","url":null,"abstract":"<div><div>Medium-chain triacylglycerol (MCT) is a healthy lipid mainly composed of medium-chain fatty acids (MCFA), which has been proven to have physiological activities in improving metabolic disorders, reducing blood cholesterol, and controlling weight. Brown adipose tissue (BAT) has been regarded as a potential organ to fight obesity due to the function of thermogenesis and energy dissipation. Previous reports found that a diet rich in MCT contributed to the activation of BAT. However, the potential mechanism between MCT and BAT remains unknown. In the current study, MCFA was applied on C3H/10T1/2 cells differentiated brown adipocytes, and MCT was applied on high-fat diet (HFD) induced obese mice. The results showed that MCFA and MCT induced browning of adipocytes and activation of BAT, significantly increased the enrichment of mitochondria, and significantly reduced intracellular lipid accumulation and body weights <em>in vivo</em> and <em>in vitro</em>. Mechanically, MCT significantly increased the level of UCP1, AMPK, and the downstream signaling factors of <em>Pgc1α</em> and <em>Ulk1</em>, further significantly elevated the brown differentiation factor of <em>Pparγ</em>. Moreover, The AMPK inhibitor dorsomorphin partially impaired the beneficial effects caused by MCT. In conclusion, this study proved that AMPK is the potential target of MCT to induce BAT activation and provided theoretical evidence for the application of MCT in the future.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"141 ","pages":"Article 109914"},"PeriodicalIF":4.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}