Journal of Nutritional Biochemistry最新文献

{"title":"Strontium and Barium in Deciduous Teeth and Infant Feeding Methods: A Birth Cohort Adjunct Study to the Japan Environment and Children's Study.","authors":"Aya Hisada, Yu-Ki Tanaka, Akifumi Eguchi, Midori Yamamoto, Kenichi Sakurai, Emiko Todaka, Noriyuki Suzuki, Yasumitsu Ogra, Atsushi Kasamatsu, Katsuhiro Uzawa, Chisato Mori","doi":"10.1016/j.jnutbio.2025.110035","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.110035","url":null,"abstract":"<p><strong>Background: </strong>Deciduous teeth are a unique medium that record exposure to various elements over time, as they are formed during fetal and infantile periods. We aimed to evaluate the association between infant feeding methods and changes in the distribution of Barium (Ba) and Strontium (Sr) in deciduous teeth of children.</p><p><strong>Methods: </strong>The current analysis included 216 children around 6-7 years of age whose deciduous central incisor teeth were provided between March 2018 and February 2020, as an adjunct study of the Japan Environment and Children's study. Ba, Sr, and Calcium (Ca) levels in the deciduous teeth of these children were measured using laser ablation-inductively coupled plasma-mass spectrometry. We estimated the association between feeding methods or season of birth and the Ba to Ca (Ba:Ca) and Sr to Ca (Sr:Ca) ratios using multivariable linear mixed effects models.</p><p><strong>Results: </strong>The Ba:Ca and Sr:Ca ratios were higher in the formula feeding group than in the breastfeeding group during the postnatal period [Ba:Ca: β=0.242 (95% CI: 0.149-0.335), Sr:Ca: β=0.223 (95% CI: 0.160-0.287) for formula group] and prenatal period [Ba:Ca: β=0.145 (95% CI: 0.062-0.227), Sr:Ca: β=0.152 (95% CI: 0.093-0.210) for formula group]. Furthermore, the Sr:Ca ratio tended to be higher for summer-born children than for winter-born ones.</p><p><strong>Conclusion: </strong>The Ba:Ca and Sr:Ca ratios in deciduous teeth can serve as useful biomarkers for estimating feeding methods. Deciduous central incisors may serve as useful indicators of longitudinal elemental exposure and nutritional status from the prenatal to postnatal periods.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"110035"},"PeriodicalIF":4.8,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxidative stress and caspase 3/Gsdme-dependent pyroptosis contributes to high fat diet induced-intestinal inflammation and lipotoxicity via Srebp1 cleavage at D444 site by caspase 3.","authors":"An-Gen Yu, Xiao-Lei Wei, Ester Zito, Hua Zheng, Chong-Chao Zhong, Yu-Long Gong, Zhi Luo","doi":"10.1016/j.jnutbio.2025.110033","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.110033","url":null,"abstract":"<p><p>Excessive dietary fat intake was associated with intestinal inflammation and lipotoxicity, but the underlying mechanism remained elusive. In this study, we investigated the effects and mechanisms of dietary fat addition on intestinal inflammation and lipid metabolism by using yellow catfish Pelteobagrus fulvidraco, a freshwater teleost fish of ecological and economic importance. Here, we found that high fat diet (HFD) and fatty acid (FA) incubation induced intestinal lipotoxicity and barrier damage, activated oxidative stress and induced intestinal inflammation by activating cysteinyl aspartate specific proteinase 3 (caspase 3)/ gasdermin E (Gsdme) -dependent pyroptosis; oxidative stress mediated FA-induced pyroptosis, lipogenic metabolism and lipid accumulation; high dietary fat induced full-length sterol regulatory element binding protein 1 (Srebp1) cleavage by caspase 3, which in turn produced the active cleaved forms of Srebp1, and accordingly contributed to lipogenic metabolism and lipid accumulation; D444 was identified as the cleavage site of Srebp1 by caspase 3. Mechanistically, overexpression of the cleaved Srebp1 (Flag-N-Srebp1) by caspase 3 increased the activities of the promoters of lipogenic genes [fatty acid synthase (fas), acetyl CoA carboxylase (acca) and stearoyl-CoA desaturase 1 (scd1)], thereby up-regulating lipogenic metabolism and inducing lipid accumulation. Thus, our study elucidated the novel mechanism of HFD inducing inflammation and lipotoxicity, and found that oxidative stress and caspase 3/ GsdmE-dependent pyroptosis played important roles in these processes.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"110033"},"PeriodicalIF":4.8,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adequate selenium nutrition attenuated the association between cadmium and renal health in U.S. adults: Evidence from NHANES data (2011-2018).","authors":"Junying Zhu, Shimiao Dai, Chenggang Yang, Ziyu Han, Zhan Shi, Yutian Luo, Alexey A Tinkov, Longjian Liu, Ji-Chang Zhou","doi":"10.1016/j.jnutbio.2025.110032","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.110032","url":null,"abstract":"<p><p>Cadmium (Cd) exposure is well-known to be hazardous to renal function. Although animal experiments suggest that selenium (Se) supplementation has beneficial effects on Cd-induced organ damage, epidemiological evidence on the mitigation of Se on Cd-induced renal damage is still insufficient. We used data from the National Health and Nutrition Examination Survey (NHANES) cycles from 2011 to 2018, and performed survey-weighted linear regression, logistic regression, and restricted cubic spline analyses to evaluate the associations of urine Cd (UCd), blood Cd (BCd), daily Se intake (DSe), and blood Se (BSe) with estimated glomerular filtration rate (eGFR), urine albumin creatinine ratio (UACR), and CKD risk, including the effects of DSe and BSe on the associations of UCd and BCd with renal health. In our study, UCd and BSe were positively associated with eGFR, but negatively associated with CKD risk. UCd and BCd were positively associated with UACR, and BCd was positively associated with CKD risk. No independent associations of DSe with eGFR, UACR, and CKD risk were observed. Additionally, we observed that the associations of Cd exposure with renal injury indicators and CKD risk were attenuated in participants with adequate DSe levels and/or higher BSe levels. Our findings suggested that Cd exposure was associated with renal impairment and CKD risk, and maintaining adequate DSe and good Se status attenuated the associations of Cd exposure with renal health. Further research is needed to evaluate the health effects of interactions between Se and Cd.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"110032"},"PeriodicalIF":4.8,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Theaflavin-3,3´-Digallate Prevents Alcoholic Liver Injury by Suppressing Hepatic TLR4/NF-κB Signaling and Modulating the Gut-Liver Axis in Mice.","authors":"Meng-Ge Tang, Li-Gui Xiong, Jian-An Huang, Yukihiko Hara, Sheng Zhang, Zhong-Hua Liu, Ai-Ling Liu","doi":"10.1016/j.jnutbio.2025.110031","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.110031","url":null,"abstract":"<p><p>Dietary intervention is crucial for the clinical management of alcoholic liver injury. As the primary bioactive component in fermented tea, theaflavin-3,3'-digallate (TF3) possesses potent antioxidative and anti-inflammatory capacities, but its protective mechanisms against alcoholic liver injury via the gut-liver axis require systematic elucidation. This study evaluated the protective effects and underlying mechanisms of high-purity TF3 (2.5/5/10 mg/kg, 12-week oral gavage) against alcoholic liver injury in C57BL/6J mice. TF3 administration significantly reduced serum lipids, attenuated hepatic steatosis, and suppressed oxidative stress and pro-inflammatory cytokine production in alcohol-fed mice. Mechanistically, TF3 enhanced intestinal barrier integrity by upregulating tight junction proteins in the colon and ileum, increased beneficial microbiota like Lactobacillus and Bifidobacterium, and modulated microbial metabolites including short-chain fatty acids (SCFAs) and tryptophan. These changes reduced circulating lipopolysaccharide (LPS), suppressed hepatic toll-like receptor 4 (TLR4)/nuclear factor kappa-B (NF-κB) pathway activation, and ultimately ameliorated hepatic inflammation while enhancing oxidative stress regulation. This work reveals novel mechanisms of TF3 in preventing alcoholic liver injury, providing a theoretical foundation for dietary applications.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"110031"},"PeriodicalIF":4.8,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144642853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Baicalein reduces cardiac inflammatory infiltration in EAM mice by blocking the CCL2-CCR2 signaling axis through its binding with TNF-α and CCR2.","authors":"Huimin Tong, Shuang Wang, Meng Liu, Tiantian Wang, Xihui Jia, Yuanyuan Li, Dongyu Yu, Ling Li","doi":"10.1016/j.jnutbio.2025.110029","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.110029","url":null,"abstract":"<p><strong>Background: </strong>Myocarditis refers to localized or diffuse inflammatory lesions of the myocardium. Experimental autoimmune myocarditis (EAM) in mice is commonly utilized as an animal model for studying the pathogenesis of myocarditis. Baicalein (BAI), the main active component extracted from Scutellaria baicalensis root, has been proven to possess diverse effects such as anti-inflammatory, anti-tumour, and antioxidant activities. However, further investigation is warranted to elucidate the mechanism of action underlying BAI's efficacy in EAM.</p><p><strong>Purpose: </strong>The aim of this study is to the potential of BAI in combination with TNF-α to downregulate the TNF-α/TNFR1-AP-1 signaling pathway. Furthermore, we will explore whether BAI exhibits an inhibitory effect on the CCL2/CCR2-ROCK1 signaling pathway.</p><p><strong>Study design and methods: </strong>In this study, we employed the EAM animal model to investigate the inhibitory effect of BAI on macrophage and Th1 cell chemotaxis towards cardiac tissue in EAM mice. Techniques such as HE staining, immunofluorescence, and other methods were utilized for assessment. Additionally, computer-simulated molecular docking, Streptavidin pull-down, and co-immunoprecipitation experiments were conducted to explore the potential binding of BAI with TNF-α and CCR2. Furthermore, real-time quantitative polymerase chain reaction (qPCR), western blotting, and flow cytometry were employed to elucidate the impact of BAI on the TNF-α/TNFR1-CCL2/CCR2 signaling pathway.</p><p><strong>Results: </strong>BAI suppressed the expression of chemokine CCL2 in EAM mouse myocardial tissue and attenuated the infiltration of macrophages and Th1 cells. In vitro, BAI exhibited binding affinity to TNF-α, leading to downregulation of the TNF-α/TNFR1-AP-1 signaling pathway and subsequent inhibition of CCL2 secretion by macrophages and vascular endothelial cells. Additionally, BAI demonstrated binding capability to CCR2, resulting in downregulation of the CCL2/CCR2-ROCK1 pathway and consequent inhibition of chemotactic migration of macrophages and Th1 cells.</p><p><strong>Conclusion: </strong>This study demonstrates that BAI can downregulate the secretion of CCL2 and the CCL2/CCR2-ROCK1 signaling pathway by binding with TNF-α and CCR2, thereby inhibiting the migration of macrophages and Th1 cells to the lesion site, thus alleviating the inflammation severity in EAM.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"110029"},"PeriodicalIF":4.8,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuroregulatory effect of royal jelly.","authors":"Feng Zhu, Ruili Yang, Biao He, Yi Xu, Hui-Li Wang","doi":"10.1016/j.jnutbio.2025.110028","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.110028","url":null,"abstract":"<p><p>Royal jelly (RJ), a nutrient-rich secretion fed exclusively to queen honeybees, exhibits diverse biological activities. Due to limited insights into its effects on central nervous system (CNS)-related functions and pathologies, this review attempts to recapitalize the recent advances of neuroregulatory function of RJ in mammals and humans. Another central aim of this review is to summarize the known mechanisms underlying the neuroregulatory effect of RJ. RJ components were first discussed concerning their link to neuroprotection, with a focus on the queen bee acid (QBA, 10-HDA, trans-10-hydroxy-2-decenoic acid), MRJPs (major royal jelly proteins), and microbiota. The neuroregulatory implications of RJ are then categorized into three domains: fundamental CNS functions, including learning and memory, in vitro neuronal cell activity and social behavior; neurological disorders such as Alzheimer's disease (AD), Parkinson's disease (PD), anxiety and depression; neurotoxic events. In terms of mechanistic insights, RJ modulates neuroinflammation, a pivotal driver of neurological disorders, and acts as an HDAC (histone deacetylase) inhibitor among other epigenetic regulators, influencing gene expression in the CNS. Emerging evidence also implicates the microbiota-gut-brain (MGB) axis, where RJ alters gut microbiota composition, subsequently impacting brain function. Finally, we outline critical research directions, including clinical significance, comparisons with other honeybee products, epigenetic mechanisms, and RJ's untapped role in reshaping social behaviors. This review aims to consolidate our knowledge on the neuroregulatory effects of RJ, underscoring its therapeutic potential for neurological conditions in both nutritional and pharmaceutical contexts.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"110028"},"PeriodicalIF":4.8,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics analyses revealed the distinct effects of calorie restriction diets with different fat-to-energy ratios on body weight and glycolipid metabolism in rats.","authors":"Jiedong Chen, Yijie Wang, Ke Sun, Yiqi Lin, Fuchuan Guo","doi":"10.1016/j.jnutbio.2025.110026","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.110026","url":null,"abstract":"<p><p>To explore the distinct effects of different fat-to-energy ratio calorie restriction (CR) diets on health, 50 male Wistar rats were randomly assigned to five groups: two ad libitum groups were fed with either normal (ND-AL) or high-fat (HF-AL) diets, and three CR groups were fed with respective low-[10% (cal) fat, LF-CR], medium-[35% (cal) fat, MF-CR] and high-[45% (cal) fat, HF-CR] fat-to-energy ratio diets for 10 weeks after obesity modeling. Among the three CR groups, HF-CR had the most pronounced weight loss effect, but it also exacerbated glycolipid metabolism disorders. In the HF-CR group, an increase in gut microbiota associated with glucose metabolism disorder (e.g. Collinsella and Enterorhabdus) was observed compared to the LF-CR group. Additionally, there was a significant decrease in the expression levels of genes involved in hepatic gluconeogenesis, glycogen synthesis, and insulin signaling in the MF-CR and HF-CR groups when compared to the LF-CR group. Further analysis indicated that Enterorhabdus may potentially regulate the gene expression of the glucokinase regulator (GCKR) by modulating choline metabolism, thereby impacting glucose metabolism. Our study emphasizes the importance of maintaining an appropriate fat-to-energy ratio in CR diets to promote healthy weight loss by balancing dietary fat, gut microbiota, and glucose metabolism.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"110026"},"PeriodicalIF":4.8,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of the early introduction of trophic donor human breast milk on the preservation of postoperative gut functions in a fasting animal model.","authors":"Ayse Tuzlaci, Fethiye Kolayli, Bahar Muezzinoglu, B Haluk Güvenç","doi":"10.1016/j.jnutbio.2025.110030","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.110030","url":null,"abstract":"<p><p>Dietary restrictions, such as avoiding oral intake until postoperative ileus resolves, can result in negative nitrogen balance, weakened immune function, and impaired digestive system performance. This study investigates the efficacy of donor human breast milk (HM) in early trophic enteral feeding and its impact on gut functions during prolonged postoperative fasting in an animal model. Male Wistar rats were deprived of food for 48 hours with free water access. After a complete ileal transection and an end-to-end intestinal anastomosis, the rats were divided into three groups: prolonged fasting (PF), early trophic feeding with HM, and feeding with commercial formula (CF). Each group was further divided into 48- and 72-hour postoperative subgroups. The study measured the number of colony-forming units in mesenteric lymph nodes, serum alkaline phosphatase (ALP) levels, and histopathological data between groups. At postoperative 48 and 72 hours, the mean apoptotic index of HM group was significantly lower than in PF group (p<0.001). A significant decrease was observed in apoptosis in HM group over time (p=0.047), while the CF (p=0.327) and PF (p=0.959) groups did not show significant changes. HM significantly prevented villus atrophy (p<0.01), and bacterial translocation to mesenteric lymph nodes (p<0.05) compared to CF and PF. Serum ALP levels, an indicator of intestinal mucosal regeneration, was significantly highest in the HM group at 72 hours compared to other groups (p=0.03). These findings suggest that HM not only maintains the structural and functional gut integrity but also promotes earlier and more effective recovery of the intestinal health.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"110030"},"PeriodicalIF":4.8,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144618558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of docosahexaenoic acid (DHA) on plasma cytokines, oxylipins, and tumor-infiltrating lymphocytes from women with breast cancer undergoing neoadjuvant chemotherapy in the DHA-WIN trial.","authors":"Jaqueline Munhoz, Gilbert Bigras, Marnie Newell, Magaly Rivas Serna, Vera Mazurak, Susan Goruk, Anil Abraham Joy, Sunita Ghosh, Kerry S Courneya, Denise G Hemmings, Catherine J Field","doi":"10.1016/j.jnutbio.2025.110025","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.110025","url":null,"abstract":"<p><strong>Background: </strong>Clinical trials on docosahexaenoic acid (DHA) supplementation and immune changes during breast cancer neoadjuvant chemotherapy (NAC) are limited. This study evaluated the impact of DHA supplementation during NAC on systemic and tumor immune modulation by assessing plasma inflammatory and cardiac damage markers, tumor-infiltrating lymphocyte (TIL) proportions, and n-6- and n-3-derived oxylipins produced in response to an ex vivo immune challenge.</p><p><strong>Methods: </strong>Venous blood was collected at baseline, 9, and 15 weeks during NAC from participants in the DHA for Women with Breast Cancer in the Neoadjuvant Setting (DHA-WIN) trial, which compared DHA-enriched algae (4.4g/day; n=23) with a placebo (n=26) over 18 weeks. Plasma markers were measured using electrochemiluminescence assays. CD4+ and CD8+ TILs were identified in tumor tissue by immunohistochemistry, and oxylipins were quantified in the supernatant of lipopolysaccharide-stimulated peripheral blood mononuclear cells via liquid chromatography-tandem mass spectrometry.</p><p><strong>Results: </strong>DHA supplementation resulted in greater increases in the plasma cytokines IFN-γ, TNF-α, and IL-17A compared to placebo (P-interaction < 0.05). In the DHA group, concentrations of these cytokines increased at 15 weeks compared to baseline (P<0.05). No differences were found between groups for other immune markers or the proportion of TILs. Compared to the placebo, DHA led to an overall increase in total oxylipin concentrations (P<0.05) and higher production of n-6 fatty acid-derived oxylipins, particularly prostanoids, and n-3 fatty acid-derived oxylipins, including 13-HdoHE.</p><p><strong>Conclusion: </strong>These results suggest that DHA may enhance immune responses by promoting an increase in oxylipin and cytokine concentrations, potentially benefiting patients during breast cancer NAC.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"110025"},"PeriodicalIF":4.8,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144618559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary supplementation with eugenol modulates lipid metabolism in finishing pigs through the TRPV1/AMPK signaling pathway.","authors":"Liping Nie, Yaxin Qiu, Xiaoling Chen, Ping Zheng, Yuheng Luo, Bing Yu, Daiwen Chen, Zhiqing Huang","doi":"10.1016/j.jnutbio.2025.110027","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.110027","url":null,"abstract":"<p><p>Eugenol (4-allyl-2-methoxyphenol), a natural phytogenic compound, exhibits physiological properties including antibacterial, anti-inflammatory, and antioxidant activities. This study aimed to evaluate the effects of dietary eugenol supplementation on lipid metabolism, carcass characteristics, and mechanism in finishing pigs. In our findings, dietary supplementation with eugenol significantly improved carcass traits in finishing pigs (P < 0.05). The eugenol-supplemented diet markedly reduced serum concentrations of total cholesterol (TC), triglycerides (TG), and low-density lipoprotein (LDL) (P < 0.05), while significantly increasing high-density lipoprotein (HDL) levels (P < 0.05). Furthermore, eugenol supplemented diet significantly decreased the content of TC and TG in muscle tissue (P < 0.05). Specifically, EUG significantly downregulated the protein expression of acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), and sterol regulatory element-binding protein (SREBP) in adipose tissue (P < 0.05). EUG treatment upregulated protein expressions of transient receptor potential vanilloid-1 (TRPV1) and P-AMPK/AMPK (P < 0.05). These results demonstrated that eugenol regulates lipid metabolism through the TRPV1/AMPK signaling pathway, effectively reducing serum TG and cholesterol levels while inhibiting lipid accumulation.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"110027"},"PeriodicalIF":4.8,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144618556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}