Ligia Soares Lima, Marcia Ribeiro, Ludmila F M F Cardozo, Rudolf Bittner, Marcelo Ribeiro-Alves, Júnia Schultz, Alexandre Soares Rosado, Paulo Emilio Correa Leite, Lia S Nakao, Leon J Schurgers, Denise Mafra
{"title":"血液透析患者维生素K水平低下与炎症和肠道微生物群的关系：一项横断面研究。","authors":"Ligia Soares Lima, Marcia Ribeiro, Ludmila F M F Cardozo, Rudolf Bittner, Marcelo Ribeiro-Alves, Júnia Schultz, Alexandre Soares Rosado, Paulo Emilio Correa Leite, Lia S Nakao, Leon J Schurgers, Denise Mafra","doi":"10.1016/j.jnutbio.2025.110139","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Vitamin K insufficiency is common in chronic kidney disease (CKD) and may be associated with gut dysbiosis, which decreases the number of vitamin K-producing bacteria. This insufficient status worsens inflammation, leading to vascular calcification and oxidative stress.</p><p><strong>Objective: </strong>This study investigates the correlation between vitamin K status and gut microbiota composition, and its association with inflammation in hemodialysis (HD) patients.</p><p><strong>Methods: </strong>In this cross-sectional study, patients were grouped based on dephosphorylated-uncarboxylated matrix gla-protein (dp-ucMGP) levels: adequate (≤ 500 pmol/L) or inadequate (> 500 pmol/L) vitamin K status. Plasma cytokines were analyzed using a multiplex assay, and uremic toxins via reverse-phase high-performance liquid chromatography (RP-HPLC). Gut microbiota composition was assessed in a subgroup using fecal DNA extraction and 16S rRNA gene sequencing on the Illumina NovaSeq PE250 platform.</p><p><strong>Results: </strong>Among 107 patients (53 [interquartile range = 16] years and 36 [interquartile range = 42] months on HD) completed the study, 70 patients (53 years, BMI, 24.2Kg/m<sup>2</sup>) exhibited insufficient vitamin K status, and 37 patients presented adequate status (52.5 years, BMI, 25.6Kg/m<sup>2</sup>). Patients with inadequate vitamin K status exhibited significantly higher levels of interleukin (IL)-6, IL-1β, granulocyte-macrophage colony-stimulating factor (GM-CSF), and granulocyte colony-stimulating factor (G-CSF) compared to patients with adequate status, reflecting elevated inflammatory marker levels in patients with insufficient vitamin K. Additionally, Bacteroides, a key vitamin K2-producing genus, was decreased in patients with inadequate vitamin K status.</p><p><strong>Conclusions: </strong>Gut dysbiosis is a consequence of CKD, which can result in reduced production of vitamin K and subsequent insufficient status. Additionally, inadequate vitamin K status may contribute to the inflammatory state in patients undergoing HD.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"110139"},"PeriodicalIF":4.9000,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Association of Poor Vitamin K Status with Inflammation and Gut Microbiota in Hemodialysis Patients: A Cross-Sectional Study.\",\"authors\":\"Ligia Soares Lima, Marcia Ribeiro, Ludmila F M F Cardozo, Rudolf Bittner, Marcelo Ribeiro-Alves, Júnia Schultz, Alexandre Soares Rosado, Paulo Emilio Correa Leite, Lia S Nakao, Leon J Schurgers, Denise Mafra\",\"doi\":\"10.1016/j.jnutbio.2025.110139\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Vitamin K insufficiency is common in chronic kidney disease (CKD) and may be associated with gut dysbiosis, which decreases the number of vitamin K-producing bacteria. This insufficient status worsens inflammation, leading to vascular calcification and oxidative stress.</p><p><strong>Objective: </strong>This study investigates the correlation between vitamin K status and gut microbiota composition, and its association with inflammation in hemodialysis (HD) patients.</p><p><strong>Methods: </strong>In this cross-sectional study, patients were grouped based on dephosphorylated-uncarboxylated matrix gla-protein (dp-ucMGP) levels: adequate (≤ 500 pmol/L) or inadequate (> 500 pmol/L) vitamin K status. Plasma cytokines were analyzed using a multiplex assay, and uremic toxins via reverse-phase high-performance liquid chromatography (RP-HPLC). Gut microbiota composition was assessed in a subgroup using fecal DNA extraction and 16S rRNA gene sequencing on the Illumina NovaSeq PE250 platform.</p><p><strong>Results: </strong>Among 107 patients (53 [interquartile range = 16] years and 36 [interquartile range = 42] months on HD) completed the study, 70 patients (53 years, BMI, 24.2Kg/m<sup>2</sup>) exhibited insufficient vitamin K status, and 37 patients presented adequate status (52.5 years, BMI, 25.6Kg/m<sup>2</sup>). Patients with inadequate vitamin K status exhibited significantly higher levels of interleukin (IL)-6, IL-1β, granulocyte-macrophage colony-stimulating factor (GM-CSF), and granulocyte colony-stimulating factor (G-CSF) compared to patients with adequate status, reflecting elevated inflammatory marker levels in patients with insufficient vitamin K. Additionally, Bacteroides, a key vitamin K2-producing genus, was decreased in patients with inadequate vitamin K status.</p><p><strong>Conclusions: </strong>Gut dysbiosis is a consequence of CKD, which can result in reduced production of vitamin K and subsequent insufficient status. Additionally, inadequate vitamin K status may contribute to the inflammatory state in patients undergoing HD.</p>\",\"PeriodicalId\":16618,\"journal\":{\"name\":\"Journal of Nutritional Biochemistry\",\"volume\":\" \",\"pages\":\"110139\"},\"PeriodicalIF\":4.9000,\"publicationDate\":\"2025-10-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Nutritional Biochemistry\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1016/j.jnutbio.2025.110139\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Nutritional Biochemistry","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.jnutbio.2025.110139","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Association of Poor Vitamin K Status with Inflammation and Gut Microbiota in Hemodialysis Patients: A Cross-Sectional Study.
Background: Vitamin K insufficiency is common in chronic kidney disease (CKD) and may be associated with gut dysbiosis, which decreases the number of vitamin K-producing bacteria. This insufficient status worsens inflammation, leading to vascular calcification and oxidative stress.
Objective: This study investigates the correlation between vitamin K status and gut microbiota composition, and its association with inflammation in hemodialysis (HD) patients.
Methods: In this cross-sectional study, patients were grouped based on dephosphorylated-uncarboxylated matrix gla-protein (dp-ucMGP) levels: adequate (≤ 500 pmol/L) or inadequate (> 500 pmol/L) vitamin K status. Plasma cytokines were analyzed using a multiplex assay, and uremic toxins via reverse-phase high-performance liquid chromatography (RP-HPLC). Gut microbiota composition was assessed in a subgroup using fecal DNA extraction and 16S rRNA gene sequencing on the Illumina NovaSeq PE250 platform.
Results: Among 107 patients (53 [interquartile range = 16] years and 36 [interquartile range = 42] months on HD) completed the study, 70 patients (53 years, BMI, 24.2Kg/m2) exhibited insufficient vitamin K status, and 37 patients presented adequate status (52.5 years, BMI, 25.6Kg/m2). Patients with inadequate vitamin K status exhibited significantly higher levels of interleukin (IL)-6, IL-1β, granulocyte-macrophage colony-stimulating factor (GM-CSF), and granulocyte colony-stimulating factor (G-CSF) compared to patients with adequate status, reflecting elevated inflammatory marker levels in patients with insufficient vitamin K. Additionally, Bacteroides, a key vitamin K2-producing genus, was decreased in patients with inadequate vitamin K status.
Conclusions: Gut dysbiosis is a consequence of CKD, which can result in reduced production of vitamin K and subsequent insufficient status. Additionally, inadequate vitamin K status may contribute to the inflammatory state in patients undergoing HD.
期刊介绍：
Devoted to advancements in nutritional sciences, The Journal of Nutritional Biochemistry presents experimental nutrition research as it relates to: biochemistry, molecular biology, toxicology, or physiology.
Rigorous reviews by an international editorial board of distinguished scientists ensure publication of the most current and key research being conducted in nutrition at the cellular, animal and human level. In addition to its monthly features of critical reviews and research articles, The Journal of Nutritional Biochemistry also periodically publishes emerging issues, experimental methods, and other types of articles.
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