Association of Poor Vitamin K Status with Inflammation and Gut Microbiota in Hemodialysis Patients: A Cross-Sectional Study.

IF 4.9 2区医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Nutritional Biochemistry Pub Date : 2025-10-04 DOI:10.1016/j.jnutbio.2025.110139

Ligia Soares Lima, Marcia Ribeiro, Ludmila F M F Cardozo, Rudolf Bittner, Marcelo Ribeiro-Alves, Júnia Schultz, Alexandre Soares Rosado, Paulo Emilio Correa Leite, Lia S Nakao, Leon J Schurgers, Denise Mafra

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引用次数: 0

Abstract

Background: Vitamin K insufficiency is common in chronic kidney disease (CKD) and may be associated with gut dysbiosis, which decreases the number of vitamin K-producing bacteria. This insufficient status worsens inflammation, leading to vascular calcification and oxidative stress.

Objective: This study investigates the correlation between vitamin K status and gut microbiota composition, and its association with inflammation in hemodialysis (HD) patients.

Methods: In this cross-sectional study, patients were grouped based on dephosphorylated-uncarboxylated matrix gla-protein (dp-ucMGP) levels: adequate (≤ 500 pmol/L) or inadequate (> 500 pmol/L) vitamin K status. Plasma cytokines were analyzed using a multiplex assay, and uremic toxins via reverse-phase high-performance liquid chromatography (RP-HPLC). Gut microbiota composition was assessed in a subgroup using fecal DNA extraction and 16S rRNA gene sequencing on the Illumina NovaSeq PE250 platform.

Results: Among 107 patients (53 [interquartile range = 16] years and 36 [interquartile range = 42] months on HD) completed the study, 70 patients (53 years, BMI, 24.2Kg/m²) exhibited insufficient vitamin K status, and 37 patients presented adequate status (52.5 years, BMI, 25.6Kg/m²). Patients with inadequate vitamin K status exhibited significantly higher levels of interleukin (IL)-6, IL-1β, granulocyte-macrophage colony-stimulating factor (GM-CSF), and granulocyte colony-stimulating factor (G-CSF) compared to patients with adequate status, reflecting elevated inflammatory marker levels in patients with insufficient vitamin K. Additionally, Bacteroides, a key vitamin K2-producing genus, was decreased in patients with inadequate vitamin K status.

Conclusions: Gut dysbiosis is a consequence of CKD, which can result in reduced production of vitamin K and subsequent insufficient status. Additionally, inadequate vitamin K status may contribute to the inflammatory state in patients undergoing HD.

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血液透析患者维生素K水平低下与炎症和肠道微生物群的关系：一项横断面研究。

背景：维生素K不足在慢性肾脏疾病（CKD）中很常见，可能与肠道生态失调有关，肠道生态失调会减少产生维生素K的细菌的数量。这种不足状态会加重炎症，导致血管钙化和氧化应激。目的：探讨血液透析（HD）患者维生素K水平与肠道菌群组成的关系及其与炎症的关系。方法：在这项横断面研究中，患者根据去磷酸化-未羧化基质玻璃蛋白（dp-ucMGP）水平进行分组：维生素K充足（≤500 pmol/L）或不足（bb0 500 pmol/L）。血浆细胞因子采用多重检测，尿毒症毒素采用反相高效液相色谱（RP-HPLC）。在Illumina NovaSeq PE250平台上使用粪便DNA提取和16S rRNA基因测序来评估肠道微生物群组成。结果：107例患者（53[四分位数范围 = 16]岁，36[四分位数范围 = 42]个月）完成研究，70例患者（53岁，BMI, 24.2Kg/m2）维生素K状态不足，37例患者（52.5岁，BMI, 25.6Kg/m2）维生素K状态充足。与维生素K状态充足的患者相比，维生素K状态不足的患者表现出明显更高的白细胞介素(IL)-6、IL-1β、粒细胞-巨噬细胞集落刺激因子（GM-CSF）和粒细胞集落刺激因子（G-CSF）水平，这反映了维生素K不足患者炎症标志物水平升高。此外，维生素K状态不足的患者中主要的维生素k2生成属拟杆菌（Bacteroides）减少。结论：肠道生态失调是慢性肾病的结果，它可以导致维生素K的产生减少和随后的不足状态。此外，维生素K水平不足可能导致HD患者的炎症状态。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Nutritional Biochemistry 医学-生化与分子生物学

CiteScore

9.50

自引率

3.60%

发文量

237

审稿时长

68 days

期刊介绍： Devoted to advancements in nutritional sciences, The Journal of Nutritional Biochemistry presents experimental nutrition research as it relates to: biochemistry, molecular biology, toxicology, or physiology. Rigorous reviews by an international editorial board of distinguished scientists ensure publication of the most current and key research being conducted in nutrition at the cellular, animal and human level. In addition to its monthly features of critical reviews and research articles, The Journal of Nutritional Biochemistry also periodically publishes emerging issues, experimental methods, and other types of articles.