Journal of Nutritional Biochemistry最新文献

{"title":"Mitigating effects of resveratrol on cold exposed-induced cardiac mitochondrial dysfunction and lipid metabolic disorder in broilers.","authors":"Haidong Wei, Deyang Miao, Haochen Li, Hongyu Wang, Qiang Xue, Yuanyuan Liu, Yanju Bi, Jianhong Li","doi":"10.1016/j.jnutbio.2025.110133","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.110133","url":null,"abstract":"<p><p>Cold climate is a severe challenge to the sustainability of global poultry production, as it impairs the health and growth performance. Resveratrol (RES) is a natural polyphenol and has antioxidative and anti-inflammatory activities. To investigate whether RES alleviates cardiac substance metabolism disorder caused by cold exposure (CE) via regulating mitochondrial quality control. 28-day-old broilers were subjected to CE at 8±1°C for 14 days and fed the diets with 0, 250, 500, and 750 mg RES/kg feed, respectively. CE reduced body weight, and caused mitochondrial structure abnormalities and lipid droplet formation in the heart. CE elevated reactive oxygen species level, reduced mRNA and protein expression of AMPK and genes related to antioxidative function (Nrf2, HO-1, SOD1, SOD2, CAT, and GPx), mitochondrial biogenesis (Nrf1 and PGC-1α), mitochondrial dynamics (MFF, MFN1, MFN2, and OPA1), and lipolysis (PPARα, CPT1, and ACO2), promoted mRNA and protein expression of dynamin-related protein 1 and genes associated with mitophagy (PINK1, Parkin, ATG5, LC3Ⅱ/Ⅰ, p62, and Beclin1) and lipogenesis (SREBP1, FAS, ACC, and PPARγ), increased the concentrations of triglyceride, total cholesterol and LDLC, and reduced free fatty acids and HDLC concentrations in serum. RES reduced CE-caused oxidative stress and improved mitochondrial health, improving lipid metabolism by regulating the AMPK/PGC-1α/PPAR pathway in broiler heart. Therefore, this study suggests that dietary supplementation with RES, especially a 500 mg/kg dosage, could relieve CE-induced heart injury and dysfunction associated with lipid metabolism disorder by improving the Nrf2/HO-1 pathway-modulated antioxidative defense function and balancing the AMPK signaling-regulated mitochondrial quality control system in broilers.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"110133"},"PeriodicalIF":4.9,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Porcine intestinal mucosa-derived alkaline phosphatase as a functional modulator of neutrophil inflammation via adenosine A_2AR signaling.","authors":"Chenzhe Gao, Lin Zhang, Jiahui Ma, Qinyun Liu, Haiyan Zhang, Yue Pang, Dehai Li, Chenyu Xue, Weichen Hong, Munkh-Amgalan Gantumur, Mizhou Hui, Yihong Bao, Na Dong","doi":"10.1016/j.jnutbio.2025.110129","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.110129","url":null,"abstract":"<p><p>Neutrophils serve as pivotal effectors in the innate immune response, yet their dysregulated activation contributes to inflammatory pathologies. Intestinal alkaline phosphatase (IAP), a potential anti-inflammatory agent derived from porcine intestinal mucosa, remains poorly understood in its mechanistic role within neutrophils. This study elucidates that IAP catalyzes the sequential dephosphorylation of adenosine triphosphate (ATP) to adenosine, which activates the adenosine 2A receptor (A_2AR)-dependent cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA) signaling cascade. This pathway suppresses nuclear factor-kappa B (NF-κB) activation, thereby reducing proinflammatory cytokines and mediators in murine neutrophils. Exogenous adenosine mimicked the anti-inflammatory effects of IAP, mitigating lipopolysaccharide (LPS)-induced neutrophil hyperactivation by enhancing migration resolution and attenuating phagocytic hyperactivity. In vivo experiments corroborated that adenosine alleviates systemic inflammation via A_2AR signaling, as evidenced by diminished plasma TNF-α, IL-6, and CRP levels, alongside ameliorated hepatic histopathological injury. Collectively, these findings demonstrate that IAP-derived adenosine orchestrates anti-inflammatory responses through the A_2AR/cAMP/PKA/NF-κB axis, highlighting its therapeutic potential in neutrophil-mediated inflammatory disorders and valorizing intestinal mucosal waste as a sustainable source of bioactive macromolecules.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"110129"},"PeriodicalIF":4.9,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ginkgolide B alleviates HFD-induced osteoporosis in OVX mice by inhibiting oxidative stress and modulating gut microbiota.","authors":"Ning Wang, Tong Xu, Yi-Kai Li","doi":"10.1016/j.jnutbio.2025.110131","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.110131","url":null,"abstract":"<p><p>Estrogen deficiency and a high-fat diet (HFD) are both significant risk factors for osteoporosis, with HFD further exacerbating bone loss under estrogen-deficient conditions. Ginkgolide B (GB), a natural small molecule derived from Ginkgo biloba leaves, possesses various pharmacological effects; however, its role in osteoporosis induced by estrogen deficiency and HFD remains unexplored. In this study, we evaluated the effects of GB in ovariectomized (OVX) mice subjected to HFD (HFOVX model) and found that GB treatment attenuated bone loss through multiple biological changes. GB improved bone microarchitecture, increased trabecular number, and reduced bone marrow adipose tissue accumulation. Additionally, GB alleviated oxidative stress by lowering serum malondialdehyde (MDA) levels and 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels while also reducing circulating lipopolysaccharide (LPS) levels. Furthermore, GB partially modulated the gut microbiota composition in HFOVX mice, as indicated by an increased relative abundance of potentially beneficial taxa such as Lactobacillaceae (e.g., Lactobacillus) and a decreased abundance of certain families associated with gut dysbiosis, including Ruminococcaceae, Helicobacteraceae (e.g., Helicobacter), and Desulfovibrionaceae. These microbial shifts were accompanied by lower circulating LPS and oxidative stress markers, suggesting a possible link between gut microbiota alterations and systemic inflammatory status. While these findings do not establish direct causality, they support the hypothesis that GB may exert bone-protective effects, at least in part, by modulating the gut microenvironment and systemic oxidative stress. Taken together, this study provides preliminary evidence for the therapeutic potential of GB in obesity-related postmenopausal osteoporosis and lays the groundwork for future mechanistic investigations.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"110131"},"PeriodicalIF":4.9,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145182035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary α-linolenic acid intake, different food sources and risk of all-cause mortality: a prospective cohort study.","authors":"Qi Wu, Boyang Chen, Sibo Liu, Hongkun Di, Wen Hu, Tianzhu Qin, Yanjun Guo, Xiang Cheng, Liegang Liu, Zhilei Shan","doi":"10.1016/j.jnutbio.2025.110132","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.110132","url":null,"abstract":"<p><p>Evidence about the association between dietary α-linolenic acid (ALA) intake and all-cause mortality risk remains insufficient, and less is known about the contribution of its major food sources. This study aimed to investigate the associations of dietary ALA intake and its different food sources with all-cause mortality risk among adults. A total of 12,217 participants from the China Health and Nutrition Survey (CHNS) 1997-2011 and 40,217 participants from the U.S. National Health and Nutrition Examination Survey (NHANES) 1999-2018 were included. After multivariable adjustment, the highest quartile of dietary ALA intake showed a 19% lower risk of all-cause mortality compared to the lowest quartile (pooled hazard ratio [HR]: 0.81; 95% confidence interval [CI]: 0.72-0.91), including a 23% reduction in CHNS and 17% reduction in NHANES. Each 1g/d increment in dietary ALA intake was associated with a 4% lower risk of all-cause mortality (pooled HR: 0.96; 95% CI: 0.93-0.99), in a linear dose-response manner (P-nonlinear = 0.423). For major dietary ALA sources, the highest consumption of plant oils (pooled HR: 0.86; 95% CI: 0.76-0.97), beans and vegetables (0.83; 0.76-0.90), nuts (0.78; 0.68-0.89), poultry and fish (0.89; 0.79-0.99) was associated with a lower risk of all-cause mortality compared with the lowest consumption. In conclusion, higher intakes of dietary ALA and its major plant food sources were associated with a lower risk of all-cause mortality. Consuming adequate amounts of ALA from plant-based foods could play a role in reducing the risk of all-cause mortality.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"110132"},"PeriodicalIF":4.9,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145182050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One Anastomosis Mini-Gastric Bypass Decreases Desacyl Ghrelin and Improves Metabolic Profile in Male Rats with Diet-Induced Obesity.","authors":"Carlota Tuero, Amaia Rodríguez, Beatriz Ramírez, Victoria Catalán, Javier A Cienfuegos, Javier Gómez-Ambrosi, Victor Valentí, Rafael Moncada, Gema Frühbeck, Sara Becerril","doi":"10.1016/j.jnutbio.2025.110124","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.110124","url":null,"abstract":"<p><p>The potential participation of acylated (AG) and desacyl (DAG) ghrelin in insulin resistance improvement following one anastomosis mini-gastric bypass (OAGB) remains unknown. This study compares the efficacy of the OAGB and caloric restriction in the treatment of obesity and evaluates the relationship between ghrelin isoforms and insulin sensitivity post-intervention. To that end, 40 rats were used in the experimental design, 37 rats with diet-induced obesity (DIO) were subjected to either Sham (n=11), OAGB (n=6)] or dietary interventions [fed ad libitum a normal diet (n=6), a high-fat diet (n=6) or pair-fed (PFD) to the amount of food eaten by the OAGB rats (n=8)]. The OAGB surgery resulted in significant and sustained weight loss, supported by a lower body weight (p < .01) and total adiposity compared to Sham-operated and PFD controls (p < .05 for all). Although the OAGB did not lead to significant differences in relative food intake, it induced a lower food efficiency ratio (p < .01), higher rectal temperatures (p < .05), and increased Pgc1a gene expression levels (p < .05) in brown adipose tissue, suggesting an improved energy expenditure. The OAGB also ameliorated insulin sensitivity, as evidenced by a decreased plasma glucose (p < .05), insulin (p < .01) and HOMA-IR (p < .05) compared to caloric-restricted rats, without affecting the lipid profile. Plasma DAG levels significantly decreased (p < .05) one month after the OAGB, and significant correlations were observed between DAG and markers of insulin resistance, including insulin, HOMA-IR, leptin, and Adipo-IR. This study demonstrates that the OAGB leads to significant improvements in body composition and metabolic profile through mechanisms beyond caloric restriction. The effects appear to be mediated by changes in energy expenditure and adipose tissue function, potentially modulated by alterations in the gut hormone DAG.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"110124"},"PeriodicalIF":4.9,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metals Exposure, Biological Aging and Metabolic Syndrome: Exploring the Associations and Mediation Effects.","authors":"Xu Han, Ziquan Lv, Mei'an He, Xuli Wu, Yanwei Zhang, Guimiao Lin, Dongju Zou, Jinling Liu, Meilin Li, Jinquan Cheng, Jung-Jun Han, Hui Huang, Suli Huang","doi":"10.1016/j.jnutbio.2025.110130","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.110130","url":null,"abstract":"<p><p>Metals disequilibrium is crucial in the progress of metabolic syndrome (MetS), whereas the underlying mechanism remains largely unclear. Aging is closely related with the major diseases, and recent studies show environmental exposure also accelerate aging. Therefore, we aimed at investigating the mediation impact of biological age (BA) on relationships of metals exposure with MetS risk. A cross-sectional study with 1,504 participants was conducted. BA predictors were established by Klemera and Doubal method (KDM) and Mahalanobis distance, based on clinical measures. KDM-accel [the residual difference of regressing KDM-BA to chronological age] and physiological dysregulation (PD) were further calculated and recorded as biological aging indexes (BAIs). Seven plasma metals (calcium, cobalt, copper, magnesium, molybdenum, selenium, and zinc), reported to be linked with MetS in our preceding study, were involved. Multivariate linear and logistic regression models were performed to evaluate the relationships of BAIs with metals exposure or MetS risk, respectively. The mediation effect of BA linking metals to MetS was also explored. Quantiles of magnesium, molybdenum, and cobalt showed significant negative relationships with MetS risk and two BAIs (all p-trend<0.05). Significant positive associations were observed for two BAIs and MetS risk (p<0.001). Mediation analysis showed that BAIs mediated 19.15% and 13.23% of the negative associations between plasma molybdenum, magnesium and MetS risk, respectively. Our findings suggested that essential metals might reduce MetS risk via decelerating biological aging, emphasizing the significance of essential metals in prevention of aging and MetS.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"110130"},"PeriodicalIF":4.9,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dapagliflozin alleviated obesity-related follicular development dysfunction by regulating glucose metabolism and mitochondria function in granulosa cells.","authors":"Qing Liu, Xiaolin Chen, Xiaoling Li, Lu Han, Zhuoni Xiao","doi":"10.1016/j.jnutbio.2025.110126","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.110126","url":null,"abstract":"<p><p>Dapagliflozin (DAPA), an inhibitor of the sodium-glucose cotransporter 2 (SGLT2), modulates intracellular glucose homeostasis. Previous studies have demonstrated that DAPA can improve body composition and metabolic disorders in obese women. However, whether DAPA could ameliorate high-fat diets (HFD)-induced female ovarian dysfunction and the precise molecular mechanisms remain unclear. The primary objective of this study is to investigate the effects of DAPA on ovarian and granulosa cells (GCs) function under high-fat conditions, as well as to elucidate the underlying mechanisms. Mice were fed an HFD to establish obesity models. After successful obesity modeling, mice were fed a diet supplemented with DAPA. KGN cells were exposed to palmitic acid (PA) and treated with DAPA. We identified that SGLT2 was expressed in ovaries. DAPA treatment resulted in reduced body weight and adiposity, as well as improvements in glucose metabolism and the normalization of estrous cycles in HFD mice. Furthermore, DAPA promoted ovarian follicle development by inhibiting the reactive oxygen species (ROS)/NOD-like receptor protein 3 (NLRP3) inflammasome pathway. In vitro, DAPA downregulated SGLT2 expression, reduced intracellular glucose overload and actic acid accumulation in PA-treated KGN cells. DAPA also mitigated mitochondrial dysfunction, enhances adenosine triphosphate production and inhibits ROS/NLRP3 pathway activation in PA-treated KGN cells. What's more, DAPA reduced the accumulation of intracellular lipid droplets and restored the expression of steroid hormone biosynthesis enzymes and after PA treatment. In conclusion, these results indicated that DAPA may attenuate glucose accumulation and enhance mitochondrial function in GCs under high-fat conditions, potentially through suppression of SGLT2 expression, ultimately reducing pyroptosis and improving follicular development in obesity.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"110126"},"PeriodicalIF":4.9,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145137656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulation of the Ubiquitin-Proteasome System by Circulating (Poly)phenol-Derived Metabolites: Implications for Chronic Diseases.","authors":"Sofia Ferreira, Regina Menezes, Ioannis Trougakos, Sentiljana Gumeni, Victor Bolanos-García, Cláudia Nunes Dos Santos, María Ángeles Ávila-Gálvez","doi":"10.1016/j.jnutbio.2025.110127","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.110127","url":null,"abstract":"<p><p>The ubiquitin-proteasome system (UPS) is a key cellular mechanism responsible for protein turnover, with essential roles in cell cycle regulation, gene expression, and responses to oxidative and inflammatory stress. UPS dysregulation is implicated in the pathogenesis of multiple chronic diseases, including neurodegenerative, cardiovascular, and oncological disorders. This review examines the emerging role of circulating polyphenol-derived metabolites, such as valerolactones (from flavan-3-ols), benzoic acid derivatives, urolithins (from ellagitannins), and hydroxycinnamic acids (e.g., ferulic and caffeic acids), as modulators of UPS activity. We summarize experimental evidence demonstrating their ability to affect proteasome function through diverse mechanisms, including autophagy induction, modulation of ubiquitination-related enzymes, and attenuation of oxidative or inflammatory signals. While bioavailability studies have described the absorption and metabolism of dietary polyphenols, less is known about which specific metabolites reach target tissues at biologically active concentrations capable of modulating the UPS. In addition, current knowledge is primarily based on cellular models (e.g., endothelial cells, myotubes, macrophages, cancer cell lines) and in vivo studies in rodents, often using supraphysiological doses or isolated compounds. To translate these findings into therapeutic applications, further research is needed to integrate mechanistic insights from preclinical models with data from human clinical studies, taking into account physiologically relevant concentrations, chronic exposure patterns, and tissue-specific distribution. Such approaches could help unlock the potential of (poly)phenol-derived metabolites as regulators of proteostasis in chronic disease.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"110127"},"PeriodicalIF":4.9,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145137794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interscapular white adipose tissue: an interesting depot for browning research in rodents.","authors":"Estefanía García-Ruiz, Bàrbara Reynés, Enzo Ceresi, Andreu Palou, Paula Oliver","doi":"10.1016/j.jnutbio.2025.110128","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.110128","url":null,"abstract":"<p><p>Interscapular brown adipose tissue is surrounded by a white fat depot, the interscapular WAT (isWAT), which has received little attention. Here, we have characterized isWAT and the impact of chronic intake of a very high-fat diet, the cafeteria diet, on isWAT browning capacity. The effect of high-fat diets on WAT browning is controversial, however, some groups have described increased WAT UCP1 expression, probably to counteract increased adiposity. We analyzed mRNA expression of key brown/brite markers and lipid metabolism genes, and of selected proteins in isWAT of control, cafeteria-obese and post-cafeteria rats. Morphological/immunohistochemical characterization was also performed. Results present isWAT as a depot with a molecular profile close to that of browning-prone white fat depots (inguinal and retroperitoneal), which also shares key features with BAT, including UCP1 expression (mRNA/protein). The cafeteria diet intake induced mRNA expression of browning/thermogenic genes, which was restored after the diet was withdrawn, and increased expression of key genes involved in lipid catabolism in isWAT. Moreover, basal mRNA expression of brown/brite markers was greater than in other white depots and higher UCP1 protein levels were observed compared to the browning-prone retroperitoneal depot. Thus, isWAT appears as an interesting depot to be taken into consideration for lipid research and to better characterize browning regulation.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"110128"},"PeriodicalIF":4.9,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145137739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interesterified Fat Exposure Induces Metabolic and Locomotor Dysfunction in Drosophila melanogaster: Mechanistic Links to Oxidative Stress and Apoptotic Pathways.","authors":"Luana Barreto Meichtry, Stephanie Pereira Torres, Dieniffer Espinosa Janner, Eliana Jardim Fernandes, Pamela Piardi de Almeida, Elize Aparecida Santos Musachio, Mustafa Munir Mustafa Dahleh, Marina Prigol","doi":"10.1016/j.jnutbio.2025.110115","DOIUrl":"https://doi.org/10.1016/j.jnutbio.2025.110115","url":null,"abstract":"<p><p>To understand the impacts of interesterified fat (IF) on health, in this study, we investigated the effects of exposure to IF on behavioral and molecular parameters in D. melanogaster. For this purpose, flies were exposed to diets containing IF and non-interesterified fat (NIF), at 10 and 20% concentrations for 7 days. Exposure to IF resulted in significant alterations, including increased triglyceride levels and lipid peroxidation (particularly at 20%), reduced lifespan, impaired locomotor activity, and decreased antioxidant enzyme activities (SOD and CAT) at both concentrations. Conversely, IF exposure led to increased GST activity, enhanced Nrf2 and Hsp70 immunoreactivity, and elevated p38/total p38 ratio at 20%, culminating in a marked reduction in cell viability. NIF exposure also produced adverse effects, such as increased triglyceride levels and reduced lifespan (10% and 20%). It was associated with higher lipid peroxidation and reduced Nrf2 immunoreactivity at both concentrations. At 10%, NIF increased Hsp70 immunoreactivity and phospho-Akt levels, and decreased the phospho-p38/total p38 ratio, which remained low at 20%. In summary, exposure to both IF and NIF was detrimental to flies, inducing metabolic and locomotor impairments linked to oxidative stress and apoptotic mechanisms. However, the adverse effects were more pronounced with IF, indicating its greater toxicity in this model.</p>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":" ","pages":"110115"},"PeriodicalIF":4.9,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145124971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}