Journal of Nutritional Biochemistry最新文献

{"title":"Resveratrol protection against cardiac remodeling and ischemia-reperfusion injury associated with hepatic lipid metabolism ameliorations and adipose tissue autotaxin pathway inhibition in a diet induced prediabetic female rat model","authors":"Alexis Jouenne ,&nbsp;Joevin Sourdon ,&nbsp;Isabelle Varlet ,&nbsp;Christophe Vilmen ,&nbsp;Frank Kober ,&nbsp;Jean-François Landrier ,&nbsp;Monique Bernard ,&nbsp;Martine Desrois","doi":"10.1016/j.jnutbio.2025.109992","DOIUrl":"10.1016/j.jnutbio.2025.109992","url":null,"abstract":"<div><div>Prediabetes is associated with an increased CV risk, especially for women. Recent evidence highlights the importance of liver and adipose tissue (AT) in its stratification. Current therapeutic strategies lack cardioprotective effects in this context. We evaluated the cardioprotective effects of resveratrol supplementation versus diet intervention and their association with hepatic and AT alterations in prediabetic female rats submitted to a high-fat-high-sucrose diet (HFS). Female Wistar rats were divided into 4 groups fed for 5 months with: standard diet (CTRL), HFS diet (HFS), HFS diet supplemented for the last 2 months with resveratrol (RSV) or 2 months of standard diet after 3 months of HFS diet (RSD). <em>In vivo</em> MRI was performed to study cardiac morphology, function and perfusion. Tolerance to ischemia-reperfusion (IR) injury was investigated <em>ex vivo</em> by a simultaneous measurement of cardiac function and energy metabolism with ³¹P MRS. Hepatic steatosis, along with hepatic and AT lipid metabolism and inflammation gene expression, was studied. HFS induced glucose intolerance, hepatic steatosis, higher diastolic LV volume, wall thickness, heart weight to tibia length ratio and altered myocardial tolerance to IR, with reduced function and energy metabolism. Both approaches improved glucose tolerance, reduced hepatic steatosis with lipid oxidation genes expression and ameliorated myocardial tolerance to IR. However, only RSV inhibited deleterious cardiac remodeling and autotaxin AT expression and plasma levels induced by HFS diet. RSV exhibited better cardioprotection than RSD, along with decreased hepatic steatosis and AT derived ATX, suggesting its protective potential against early cardiac alterations associated with prediabetes and NAFLD.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"144 ","pages":"Article 109992"},"PeriodicalIF":4.8,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of viable and inactivated Lactobacillus rhamnosus GG administration on the prevention of diet-induced obesity in rats: Implication of white and brown adipose tissue and influence of bacterial viability","authors":"Laura Arellano-García ,&nbsp;María P. Portillo ,&nbsp;J. Alfredo Martínez ,&nbsp;Stéphanie Krisa ,&nbsp;Iñaki Milton-Laskibar","doi":"10.1016/j.jnutbio.2025.109982","DOIUrl":"10.1016/j.jnutbio.2025.109982","url":null,"abstract":"<div><div>Obesity is one of the most prevalent chronic metabolic alterations worldwide being highly related to an increased risk for further associated co-morbidities. Current evidence indicates that subjects with obesity have a distinct gut microbiota signature, emphasizing gut microbiota composition as a potential mediating factor. The aim of this research is to evaluate the potential effects of viable and heat-inactivated <em>Lactobacillus rhamnosus</em> GG in the prevention of diet-induced obesity in a rat model. The administration of the probiotic or its heat-inactivated postbiotic partially prevented diet-induced WAT increase in a similar manner. While viable probiotic administration resulted in a reduced lipid uptake (LPL) and <em>de novo</em> lipogenesis (FAS), along with enhanced lipolysis (ATGL) in WAT, its heat-inactivated postbiotic mainly acted reducing <em>de novo</em> lipogenesis. Additionally, the obtained results demonstrated that probiotic administration enhanced thermogenesis (UCP1) and fatty acid oxidation (CPT-1b) on BAT, as well as upregulated several markers involved in mitochondrial biogenesis (p38 MAPK, NRF1 and CS). By contrast, despite the administration of the postbiotic upregulated thermogenesis and fatty acid oxidation in a comparable manner as the probiotic, these results were not accompanied by changes in mitochondrial biogenesis markers. These results indicate that under the specific experimental conditions tested, both the administration of viable and heat-inactivated <em>Lactobacillus rhamnosus</em> GG present valuable potential for preventing diet-induced WAT mass increase in rats. While both treatments exerted similar effects on WAT and BAT, subtle differences that may derive from bacterial viability were observed in the involved mechanisms of action.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"144 ","pages":"Article 109982"},"PeriodicalIF":4.8,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of competitive binding of the essential trace elements zinc, iron, copper, and manganese by gastrointestinal mucins and the effect on their absorption in vitro","authors":"Maria Maares ,&nbsp;Vincent Einhorn ,&nbsp;Jacqueline Behrendt ,&nbsp;Matthias Marczynski ,&nbsp;Christoph Schüßler ,&nbsp;Oliver Lieleg ,&nbsp;Hajo Haase","doi":"10.1016/j.jnutbio.2025.109983","DOIUrl":"10.1016/j.jnutbio.2025.109983","url":null,"abstract":"<div><div>The molecular mechanism by which the essential trace elements (TEs) zinc (Zn), iron (Fe), copper (Cu), and manganese (Mn) interact during their uptake, particularly their competition for interaction with the gastrointestinal mucus layer, remains unclear. Thus, in this study we investigated the binding of TEs to gastrointestinal mucins individually and interdependently and analyzed the impact of mucus on their intestinal absorption. Cell-free binding and competition experiments with commercially available mucin and native MUC2 and MUC5AC from porcine gastrointestinal tract showed high TE-binding capacity (Fe&gt;Zn&gt;Cu&gt;Mn) with an average affinity that was highest for Zn and lowest for Mn (Zn&gt;Fe&gt;Cu&gt;Mn). Gastrointestinal mucins contain high-affinity TE-binding sites with physiologically relevant affinities, and TEs mutually affected their interaction with mucins in ratios reflecting the <em>in vivo</em> situation, leading to various cases of displacement or augmented binding. Solely Fe was unaffected by other TEs, apart from Mn that facilitated the Fe-mucin interaction. The relevance of TE-binding by the mucins was underlined by transport studies with two 3D <em>in vitro</em> intestinal models: Caco-2 mono- and Caco-2/HT-29-MTX co-cultures, showing that at least for Fe and Zn, mucus provides a buffering system and increases the availability of the TEs by delivering them to the underlying enterocytes. This study provides the first systematic analysis of the competition between Zn, Fe, Cu, and Mn for binding to gastrointestinal mucins, demonstrating the importance of the mucus layer for TE absorption and that competition for their intestinal uptake already starts at the mucus barrier, with translational relevance for nutrition and (malabsorption) diseases.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"144 ","pages":"Article 109983"},"PeriodicalIF":4.8,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144234387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isovaleric acid ameliorates chronic restraint stress and resists inflammation by inhibiting NF-κB activation in mice","authors":"Changtong Guo ,&nbsp;Xiangda Shi ,&nbsp;Bingbing Luo ,&nbsp;Yuxuan Yang ,&nbsp;Jian Huang ,&nbsp;Jiale Xu ,&nbsp;Rong Zheng ,&nbsp;Siwen Jiang ,&nbsp;Jin Chai","doi":"10.1016/j.jnutbio.2025.109955","DOIUrl":"10.1016/j.jnutbio.2025.109955","url":null,"abstract":"<div><div>In actual production, the restraint stress caused by crowded and narrow spaces, as well as operations such as capture and transportation, can lead to intestinal inflammation and affect animal growth performance, damaging the economic benefits of livestock farms. Isovaleric acid, a type of short-chain fatty acid (SCFA), has been found to be useful in enhancing calf digestion and inhibiting the expression of inflammatory factors. In this study, we first constructed a mouse model of chronic restraint stress (CRS) and fed a diet supplemented with sodium isovalerate to mice with CRS to investigate the mitigating effect of exogenously added isovalerate on mice with CRS. An inflammation model was then constructed using piglet jejunal epithelial cell line-J2 (IPEC-J2) to simulate intestinal inflammation after stress, and to investigate the mitigating effect of isovaleric acid on inflammation as well as the repairing effect on intestinal barrier. The results showed that feeding sodium isovalerate significantly increased the weight gain as well as the body weight growth rate of CRS mice from 1-28 days (<em>P&lt;.</em>05), and extremely significantly decreased the feed intake and feed to gain ratio of CRS mice (<em>P&lt;.</em>01). In addition, feeding sodium isovalerate ameliorated the decrease in organ index in mice caused by CRS and significantly increased leg muscle index in CRS mice (<em>P&lt;.</em>05). Meanwhile, feeding sodium isovalerate improved depressive behavior and promoted 5-HT secretion in the brain and hypothalamus of CRS mice. Besides, feeding sodium isovalerate ameliorated intestinal inflammation in CRS mice, as evidenced by extremely significantly decreased the expression of colonic inflammatory factors and increased the expression of tight junction proteins (<em>P&lt;.</em>01). It was found that the addition of isovaleric acid extremely significantly reduced the expression of inflammatory factors and the phosphorylation level of NF-κB p65 in inflammatory cells (<em>P&lt;.</em>01), extremely significantly increased the survival rate of inflammatory cells (<em>P&lt;.</em>01) and the expression of tight junction proteins (<em>P&lt;.</em>05), improved the morphology of inflammatory cells. After the addition of short-chain fatty acid receptor GPR41 and GPR43 antagonists, the resistance effect of isovaleric acid on inflammation was inhibited. The above results suggest that isovaleric acid has an ameliorating effect on CRS in mice and inhibits NF-κB activation thereby exerting its anti-inflammatory effect.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"144 ","pages":"Article 109955"},"PeriodicalIF":4.8,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"6-shogaol, a bioactive component of ginger, alleviates aging-induced ocular inflammation and ER stress in the 25-month-old mice","authors":"Hyeyoon Eo ,&nbsp;Jiin Park ,&nbsp;In Gyoung Ju ,&nbsp;Myung Sook Oh","doi":"10.1016/j.jnutbio.2025.109980","DOIUrl":"10.1016/j.jnutbio.2025.109980","url":null,"abstract":"<div><div>In the elderly population, age-related macular degeneration (AMD) is a major cause of visual impairment, characterized by a thinner retinal pigment epithelium and loss of photoreceptors. 6-shogaol (6S), a component of dried <em>Zingiber officinale</em> Roscoe, has been studied for its multiple therapeutic effects. The current study aimed to investigate the effect of 6S supplementation on AMD. 25-month-old C57BL/6 mice were orally administered with 10 mg/kg of 6S for 28 consecutive days. The thickness of the retinal layer was measured by histological analysis. mRNA expression related to fibrosis, inflammation and endoplasmic reticulum stress was measured by real-time polymerase chain reaction. As a result, 6S increased the thickness of the retinal layer and promoted postsynaptic density protein-95 expression in the outer plexiform layer of the aged mice. Moreover, 6S suppressed ocular mRNA expression related to the fibrotic process, including transforming growth factor beta, collagen type 1 alpha 1, and alpha smooth muscle actin. Furthermore, 6S reduced pro-inflammatory cytokines including tumor necrosis factor alpha, interleukin 1 beta, cyclooxygenase-2, and inducible nitric oxide synthase in the eyeballs of aged mice. Lastly, 6S inhibited ocular endoplasmic reticulum stress measured by mRNA expression of C/EBP homologous protein and spliced X-box binding protein-1 in the aged mice. Taken together, these findings suggest that 6S and dried ginger could be a potential nutraceutical candidate for AMD or other age-related eye diseases.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"144 ","pages":"Article 109980"},"PeriodicalIF":4.8,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Benzyl isothiocyanate ameliorates hepatic insulin resistance in mice with high-fat diet-induced nonalcoholic fatty liver disease","authors":"Chia-Wen Lo ,&nbsp;Jyun-Lin Lee ,&nbsp;Wei-Ting Tsai ,&nbsp;Chin-Shiu Huang ,&nbsp;Ya-Chen Yang ,&nbsp;Chong-Kuei Lii ,&nbsp;Haw-Wen Chen","doi":"10.1016/j.jnutbio.2025.109981","DOIUrl":"10.1016/j.jnutbio.2025.109981","url":null,"abstract":"<div><div>The global prevalence of overweight and obesity has risen sharply over the past few decades as a result of excess calorie intake and sedentary lifestyles. Obesity increases the risk for various metabolic disorders, such as hyperlipidemia, fatty liver disease, and diabetes mellitus. Isothiocyanates, which are abundant in cruciferous vegetables, have been shown to exhibit anticancer, anti-inflammatory, and antioxidant properties. However, the efficacy of benzyl isothiocyanate (BITC) in preventing the adverse effects of obesity, such as hepatic steatosis and insulin resistance, remains uncertain. To address this knowledge gap, we assessed whether BITC protects against hepatic insulin resistance by using primary mouse hepatocytes and AML12 cells treated with palmitic acid (PA) and mice fed a high-fat diet supplemented with cholesterol and cholic acid (HFCCD). We found that the impairments in insulin sensitivity caused by PA, such as decreases in the phosphorylation of insulin receptor substrate (IRS) 1 (Tyr608), Akt, glycogen synthase kinase (GSK) 3β, and FOXO1 and increases in the expression of glucose-6-phosphatase (G6Pase) and phosphoenolpyruvate carboxykinase 1 (PEPCK) mRNA in hepatocytes, were mitigated by pretreatment with BITC. BITC also attenuated PA-induced hepatic lipid accumulation and reactive oxygen species production. In vivo, BITC significantly reduced blood glucose levels and the HOMA-IR and inhibited hepatic lipid accumulation, IRS1 phosphorylation at Ser307, and G6Pase and PEPCK expression compared with that in mice fed the HFCCD alone. These results show that BITC ameliorates the lipotoxicity associated with insulin resistance by activating the IR/IRS/Akt/FOXO1 and GSK3β pathways, which leads to decreased gluconeogenesis and increased glycogen synthesis.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"144 ","pages":"Article 109981"},"PeriodicalIF":4.8,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Something more about the role of flavonoids in aging","authors":"Salvatore Chirumbolo","doi":"10.1016/j.jnutbio.2025.109925","DOIUrl":"10.1016/j.jnutbio.2025.109925","url":null,"abstract":"","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"142 ","pages":"Article 109925"},"PeriodicalIF":4.8,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144154771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blood methylation signatures in childhood obesity and risk of cardiac hypertrophy in young adults: Findings from the BCAMS study and Mendelian randomization analysis","authors":"Xinghao Yi ,&nbsp;Haoxue Zhu ,&nbsp;Ling Zhong ,&nbsp;Mengyu He ,&nbsp;Shan Gao ,&nbsp;Ming Li","doi":"10.1016/j.jnutbio.2025.109979","DOIUrl":"10.1016/j.jnutbio.2025.109979","url":null,"abstract":"<div><div>Childhood obesity may induce epigenetic changes, affecting long-term cardiac health. However, empirical evidence remains scarce. Leveraging the prospective cohort of Beijing Child and Adolescent Metabolic Syndrome study (BCAMS), we investigated the blood DNA methylation signatures associated with childhood obesity and the future risk of cardiac hypertrophy in young adults, confirming causality with Mendelian randomization (MR). The BCAMS study followed children into adulthood. Data including blood DNA methylation profiles, along with lifestyles, blood levels of 7 adipokines and 32 amino acids were collected at baseline. Echocardiographic measurements were assessed at the 10-year follow-up. Enrichment and correlation analyses were performed, and two-sample MR analyses involving 105,268 participants from European biobanks were employed to infer causality. Cross-sectionally, we identified 376 differentially methylated sites between children with obesity and normal-weight controls (<em>n</em>=25), particularly within genes enriched in the cardiomyopathy pathway. Longitudinally, 11 childhood obesity-associated methylation sites, especially cg25835058 (<em>KAZN</em>), cg01362389 (<em>TDH</em>) and cg12099423 (<em>SLC17A9</em>), showed strong correlations with left ventricular index (LVMI) at the 10-year follow-up (<em>P</em>&lt;.0017). Additionally, these sites were associated with traditional risk factors, notably glutamine, which displayed strongest protective association with LVMI (−1.72g/m^2.7 per 1SD increase, <em>P</em>&lt;.001) when validated with the entire cohort (<em>n</em>=326). MR analysis confirmed the significant correlation between cg12099423 methylation levels and <em>SLC17A9</em> expression, and the causality between gene expression levels (<em>KAZN, TDH, SLC17A9</em>) and LVMI. Methylation associated with childhood obesity, particularly <em>SLC17A9</em>, may function as an epigenetic mechanism impacting long-term cardiac health later in life, emphasizing the significance of early intervention.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"144 ","pages":"Article 109979"},"PeriodicalIF":4.8,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbiome, a potential modulator of neuroepigenome","authors":"Ruili Yang,&nbsp;Yi Xu,&nbsp;Feng Zhu,&nbsp;Xiaojing Ma,&nbsp;Tingting Fan,&nbsp;Hui-Li Wang","doi":"10.1016/j.jnutbio.2025.109961","DOIUrl":"10.1016/j.jnutbio.2025.109961","url":null,"abstract":"<div><div>Gut microbiome has a considerable impact on the central nervous system via the “gut-brain axis.” Neuroepigenome emerges as the interface between environment and genes, potentially help conveying the signals derived from the microbiome to the brain tissue. While only a limited number of studies have implicated epigenetic roles in the gut-brain axis, this review explores how gut microbiome might impact various brain-based epigenetic mechanisms, including DNA methylation, histone modification, ncRNA and RNA methylation, notably in the context of the specific neural complications. Among the epigenetic mechanisms, histone acetylation was most well-studied with respect to its relationships with gut microbiome, exerting a dynamic influence on gene expression in the brain. Furthermore, the pathways connecting gut bacteria to neuroepigenome were summarized, highlighting the roles of metabolites such as butyrate, propionate, acetate, lactate, and folate. Of particular interest, the roles of butyrate are emphasized due to their outstanding inhibitory activity towards histone deacetylases (HDACs), among other mechanisms. It is worth noting that some indirect gut-brain pathways may also be associated with the interplay between microbiome and neuroepigenome, while IL-6 has been found to effectively transmit microbe-derived signals to histone methylation in brains. Finally, we recapitulate the future perspectives critical to understanding this gut-brain crosstalk, such as clarifying the cause-and-effect relationship, bacterial cross-feeding within the gut, and the mechanisms underlying the site-specific histone modification in the brain. Together, this review attempts to consolidate our current knowledge about the “microbiome-neuroepigenome interplay” and propose a conceptual pathway to decipher the gut-brain axis in various neurological conditions.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"144 ","pages":"Article 109961"},"PeriodicalIF":4.8,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary addition of fraxetin improved intestinal structure and growth performance in juvenile grass carp (Ctenopharyngodon idella): As a potential novel phytogenic feed additive","authors":"Jie Zhang ,&nbsp;Wei-Dan Jiang ,&nbsp;Pei Wu ,&nbsp;Yang Liu ,&nbsp;Yao-Bin Ma ,&nbsp;He-Qun Shi ,&nbsp;Sheng-Yao Kuang ,&nbsp;Shu-Wei Li ,&nbsp;Ling Tang ,&nbsp;Xiao-Qiu Zhou ,&nbsp;Lin Feng","doi":"10.1016/j.jnutbio.2025.109969","DOIUrl":"10.1016/j.jnutbio.2025.109969","url":null,"abstract":"<div><div>The well-being and development of fish are affected to varying degrees under the intensive aquaculture model, and the use of Chinese herbs for aquaculture disease control and feed additives has received increasing attention. This study examined fraxetin supplementation in juvenile grass carp to investigate its effects on growth and intestinal structure. There were 1080 grass carp (11.58±0.01 g) assigned to 6 treatments, fed with fraxetin (0, 3.9, 7.9, 15.8, 31.5, and 63.1 mg/kg) for 60 d in each treatment. In our study, appropriate fraxetin significantly increased final body weight (FBW), percent weight gain (PWG), and specific growth rate (SGR) compared to the unadded group (<em>P&lt;.05</em>), but did not affect feed efficiency (FE) (<em>P&gt;.05</em>). The administration of 7.9 mg/kg of fraxetin significantly improved fish intestinal development and body composition. Appropriate dietary fraxetin significantly enhanced intestinal digestive enzymes and brush border enzyme activity (<em>P&lt;.05</em>), decreased serum diamine oxidase (DAO) levels (<em>P&lt;.05</em>), and decreased intestinal cell apoptosis (<em>P&lt;.05</em>). Appropriate levels of fraxetin inhibited the RhoA/ROCK signaling pathway while upregulating both mRNA and protein expression of tight junction (TJ) and adherens junction (AJ) (<em>P&lt;.05</em>). These changes significantly improved apical junction complex (AJC) integrity. In conclusion, dietary supplementation with appropriate levels of fraxetin added to the diets had a facilitating effect on digestion and absorption, improved intestinal structure, and promoted fish growth performance in juvenile grass carp. In addition, the optimal dietary fraxetin levels were evaluated to be 6.06 and 7.79 mg/kg based on linear regression analysis of PWG and DAO, respectively.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"144 ","pages":"Article 109969"},"PeriodicalIF":4.8,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}