Journal of Nutritional Biochemistry最新文献

{"title":"Oral tributyrin treatment affects short-chain fatty acid transport, mucosal health, and microbiome in a mouse model of inflammatory diarrhea","authors":"Zhenghao Ye ,&nbsp;Archana Kini ,&nbsp;Qinghai Tan ,&nbsp;Sabrina Woltemate ,&nbsp;Marius Vital ,&nbsp;Katerina Nikolovska ,&nbsp;Ursula Seidler","doi":"10.1016/j.jnutbio.2025.109847","DOIUrl":"10.1016/j.jnutbio.2025.109847","url":null,"abstract":"<div><div>Butyrate may decrease intestinal inflammation and diarrhea. This study investigates the impact of oral application of sodium butyrate (NaB) and tributyrin (TB) on colonic butyrate concentration, SCFA transporter expression, colonic absorptive function, barrier properties, inflammation, and microbial composition in the colon of <em>slc26a3^−/−</em> mice, a mouse model for inflammatory diarrhea. <em>In vivo</em> fluid absorption and bicarbonate secretory rates were evaluated in the cecum and mid-colon of <em>slc26a3^+/+</em> and <em>slc26a3^−/−</em> mice before and during luminal perfusion of NaB-containing saline and were significantly stimulated in both <em>slc26a3^+/+</em> and <em>slc26a3^−/−</em> colon by NaB. Age-matched <em>slc26a3^+/+</em> and <em>slc26a3^−/−</em> mice were either fed chow containing 5% NaB or gavaged twice daily with TB for 21 d. Food and water intake, weight, and stool water content were assessed daily. Stool and tissues were collected for further analysis of SCFA production, barrier integrity, mucosal inflammation, and microbiome analysis by 16S rRNA gene sequencing. 5% NaB diet did not exert a significant impact on SCFA levels, mucus barrier, or inflammatory markers, but significantly increased oral water intake. TB gavage treatment increased the expression of SCFA transporters <em>Mct1</em> and <em>Smct1,</em> mucus content and microbial diversity, and decreased the neutrophil marker <em>Lipocalin 2, Phospholipase A2</em>, and the antimicrobial peptide <em>Reg3b</em> in the <em>slc26a3^−/−</em> cecum<em>.</em> However, TB treatment also resulted in an increase in inflammatory markers such as <em>TNFα, Il-1β</em> and <em>CD3e</em> in the wildtype mucosa. While there are some benefits with TB ingestion for barrier properties and microbial composition in the diseased cecum, potentially detrimental effects were noted in the healthy colon.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"138 ","pages":"Article 109847"},"PeriodicalIF":4.8,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Milk-derived bioactive peptides in insulin resistance and type 2 diabetes","authors":"Marjan Ramezan ,&nbsp;Pishva Arzhang ,&nbsp;Andrew C. Shin","doi":"10.1016/j.jnutbio.2025.109849","DOIUrl":"10.1016/j.jnutbio.2025.109849","url":null,"abstract":"<div><div>Diabetes is a global health issue affecting over 6% of the world and 11% of the US population. It is closely linked to insulin resistance, a pivotal factor in Type 2 diabetes development. This review explores a promising avenue for addressing insulin resistance through the lens of Milk-Derived Bioactive Peptides (MBAPs). Taken from casein or whey fractions of various milks, MBAPs exhibit diverse health-promoting properties. Specific interactions between these peptides and enzymes involved in glucose digestion and metabolism have been examined, leading to the identification of some key peptides exerting the effects.</div><div>This review emphasizes the positive impact of MBAPs on glycemic control through various mechanisms. Different cell lines have been used to investigate MBAPs' effects on insulin signaling, inflammation, and oxidative stress. Preclinical <em>in vivo</em> studies have also shown that MBAPs lower glucose, stimulate insulin, and reduce inflammation. Human trials further substantiate these findings and suggest the potential utility of milk protein hydrolysates containing MBAPs in individuals with insulin resistance or T2D to improve insulin action and glucose homeostasis.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"138 ","pages":"Article 109849"},"PeriodicalIF":4.8,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143051882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increased fecal glycocholic acid levels correlate with obesity in conjunction with the depletion of archaea: The Dosanco Health Study","authors":"Ryodai Yamamura ,&nbsp;Ryo Okubo ,&nbsp;Shigekazu Ukawa ,&nbsp;Koshi Nakamura ,&nbsp;Emiko Okada ,&nbsp;Takafumi Nakagawa ,&nbsp;Akihiro Imae ,&nbsp;Takashi Kimura ,&nbsp;Akiko Tamakoshi","doi":"10.1016/j.jnutbio.2025.109846","DOIUrl":"10.1016/j.jnutbio.2025.109846","url":null,"abstract":"<div><div>Recent studies have focused on the relationship between obesity and gut microbiota. This study aims to identify fecal components and gut bacterial species associated with different BMI categories. In this study, 538 participants aged ≥18 years were categorized into underweight, normal, and obese groups based on BMI (cutoffs: 18.5 and 25.0 kg/m²). We compared 30 fecal components among these groups and calculated correlation coefficients between each component and BMI. Participants were then divided into quartiles based on fecal component levels correlated with BMI, and the prevalence ratio (PR) of obesity was calculated, adjusted for confounding factors. We also analyzed the composition and diversity of gut microbiota and bacterial gene expression among the quartiles for each fecal component. Fecal glycocholic acid (GCA) showed a significant positive correlation with BMI. The PR for obesity in the highest quartile of fecal GCA was 3.30 (95% CI, 1.21–9.54), indicating a significantly higher risk of obesity compared to the lowest quartile. Gut microbiota analysis revealed significant differences in the abundance of Ruminococcaceae Incertae Sedis, <em>Faecalibacterium</em>, and <em>Methanobrevibacter</em>, with <em>Methanobrevibacter</em> being absent in the higher quartiles of fecal GCA. Additionally, gene expression for enzymes involved in the deconjugation of conjugated bile acids, including GCA, was downregulated in the highest quartile. Increased fecal GCA levels are positively correlated with obesity, alongside a depletion of archaea.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"139 ","pages":"Article 109846"},"PeriodicalIF":4.8,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143039624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The exploration of the relationship between hyperuricemia, gout and vitamin D deficiency","authors":"Yingdong Han ,&nbsp;Xinxin Han ,&nbsp;He Zhao ,&nbsp;Menghui Yao ,&nbsp;Tiange Xie ,&nbsp;Juan Wu ,&nbsp;Yun Zhang ,&nbsp;Xuejun Zeng","doi":"10.1016/j.jnutbio.2025.109848","DOIUrl":"10.1016/j.jnutbio.2025.109848","url":null,"abstract":"<div><div>Our study aims to provide evidence concerning the relationship between hyperuricemia, gout and Vitamin D deficiency by analyzing data from Peking Union Medical College Hospital (PUMCH), the National Health and Nutrition Examination Survey (NHANES) database, and through Mendelian randomization (MR) analyses. Sample 1 involved patients from PUMCH (<em>n</em> = 13,532), and sample 2 involved participants from NHANES (Unweighted <em>n</em> = 22,860; weight <em>n</em> = 182,829,142). Logistic regression and restricted cubic spline analyses were applied to assess above relationship. A two-sample MR analysis was performed using the genome-wide association study summary statistics to identify the causal association between gout and 25-hydroxyvitamin D (25(OH)D). The results from both samples confirmed a positive correlation among hyperuricemia, gout and risk of Vitamin D deficiency. The restricted cubic spline showed positive dose-response relationship between uric acid and risk of Vitamin D deficiency and the minimal threshold of uric acid at 307.5 umol/L and 316.1 umol/L, respectively. Mediation analysis in the sample 2 found that about 29.4% of the total effect of gout on Vitamin D deficiency were mediated by serum uric acid, and 37.1% of which were mediated by body mass index.The results of our MR analysis supported a causal association between gout (IVW β(SE), −0.843 (0.337); <em>P</em> = .0123) and 25(OH)D. Sensitivity analysis and genetic risk scores approach confirmed the robustness and reliability of the above findings. Hyperuricemia and gout are both strong indicators of increased risk of Vitamin D deficiency. Findings highlight the causal effects of gout-associated genetic variants on 25(OH)D.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"138 ","pages":"Article 109848"},"PeriodicalIF":4.8,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143039625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oleanolic acid inhibits appetite through the TGR5/cAMP signaling pathway","authors":"Shuang Liu,&nbsp;Xiaoling Chen,&nbsp;Daiwen Chen,&nbsp;Bing Yu,&nbsp;Ping Zheng,&nbsp;Yuheng Luo,&nbsp;Jun He,&nbsp;Zhiqing Huang","doi":"10.1016/j.jnutbio.2025.109844","DOIUrl":"10.1016/j.jnutbio.2025.109844","url":null,"abstract":"<div><div>Inhibition of appetite is an effective approach to fight obesity. Recently, bile acids have been reported to suppress appetite and alleviate obesity via the Takeda G protein-coupled receptor 5 (TGR5). However, whether the downstream signaling molecule cyclic adenosine monophosphate (cAMP) of TGR5 is involved in this process remains unclear. Oleanolic acid (OA) is a plant analogue of bile acids. The study aimed to explore the effect of dietary OA supplementation on appetite and to examine the role of TGR5/cAMP signaling in this process. In our study, mice were divided into four treatment groups: basal diet, 50mg/kg OA-supplemented diet, 100mg/kg OA-supplemented diet, and 30mg/kg tauroursodeoxycholic acid (TUDCA)-supplemented diet. Our results showed that dietary supplementation of OA and TUDCA both suppressed appetite. Additionally, OA and TUDCA downregulated the expression of appetite-stimulating factors while upregulating appetite-suppressing factors in the hypothalamus. Furthermore, OA was found to activate TGR5 signaling in the hypothalamus. Mechanistic studies using N38 cells revealed that OA reduced the expression and secretion of agouti-related peptide (AgRP), while inhibition of TGR5 and cAMP attenuated this effect of OA. In conclusion, our findings suggest that OA may suppress appetite through activation of the TGR5/cAMP signaling pathway in the hypothalamus.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"138 ","pages":"Article 109844"},"PeriodicalIF":4.8,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143023826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal high-fat diet alters the transcriptional rhythm in white adipose tissue of adult offspring","authors":"Lu Ding ,&nbsp;Jing Liu ,&nbsp;Liyuan Zhou ,&nbsp;Qian Zhang ,&nbsp;Jieying Liu ,&nbsp;Xinhua Xiao","doi":"10.1016/j.jnutbio.2025.109843","DOIUrl":"10.1016/j.jnutbio.2025.109843","url":null,"abstract":"<div><div>A maternal high-fat diet (HFD) deteriorates the long-term metabolic health of offspring. Circadian rhythms are crucial for regulating metabolism. However, the impact of maternal HFD on the circadian clock in white adipose tissue (WAT) remains unexplored. This study aimed to identify transcriptional rhythmic alterations in inguinal WAT of adult male offspring induced by maternal HFD. To this end, female mice were fed an HFD and their male offspring were raised on a standard chow diet until 16 weeks of age. Transcriptome was performed and the data was analyzed using CircaCompare. The results showed that maternal HFD before and throughout pregnancy significantly altered the circadian rhythm of inguinal WAT while slightly modifying the WAT clock in adult male offspring. Specifically, maternal HFD contributed to gaining rhythmicity of <em>Cry2</em>, resulted in the elevated amplitude of <em>Nr1d2</em>, and led to increased midline estimating statistic of rhythm (MESOR) of <em>Clock</em> and <em>Nr1d2</em>. Furthermore, maternal HFD changed the rhythmic pattern of metabolic genes, such as <em>Pparγ, Hacd2</em>, and <em>Acsl1</em>, which are significantly enriched in metabolic regulation pathways. In conclusion, a maternal HFD before and throughout pregnancy altered the circadian rhythm of inguinal WAT in adult offspring. These alterations may play a significant role in disturbing metabolic homeostasis, potentially leading to metabolic dysfunction in adult male offspring.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"138 ","pages":"Article 109843"},"PeriodicalIF":4.8,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143007053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liver lipid metabolism, oxidative stress, and inflammation in glutamine-supplemented ob/ob mice","authors":"Jaqueline Santos Moreira Leite ,&nbsp;Eloisa Aparecida Vilas-Boas ,&nbsp;Hilton K. Takahashi ,&nbsp;Ana Cláudia Munhoz ,&nbsp;Layanne C.C. Araújo ,&nbsp;Carla Roberta Carvalho ,&nbsp;Jose Donato Jr ,&nbsp;Rui Curi ,&nbsp;Angelo Rafael Carpinelli ,&nbsp;Vinicius Cruzat","doi":"10.1016/j.jnutbio.2025.109842","DOIUrl":"10.1016/j.jnutbio.2025.109842","url":null,"abstract":"<div><div>Glutamine availability may be reduced in chronic diseases, such as type 2 diabetes mellitus (T2DM)-induced by obesity. Herein, the antioxidant, anti-inflammatory and lipid metabolism effects of chronic oral glutamine supplementation in its free and dipeptide form were assessed in <em>ob/ob</em> mice. Adult male C57BL/6J <em>ob/ob</em> mice were supplemented with L-alanyl-L-glutamine (DIP) or free L-glutamine (GLN) in the drinking water for 40 days, whilst C57BL/6J Wild-type lean (WT) and control <em>ob/ob</em> mice (CTRL) received fresh water only. Plasma and tissue (skeletal muscle and liver) glutamine levels, and insulin resistance parameters (<em>e.g.</em>, GTT, ITT, insulin) were determined. Oxidative stress (<em>e.g.</em>, GSH system, Nrf2 translocation), inflammatory (<em>e.g.</em>, NFkB translocation, TNF-α gene expression) and lipid metabolism parameters (<em>e.g.</em>, plasma and liver triglyceride levels, SRBP-1, FAS, ACC, and ChRBP gene expression) were also analyzed. CTRL <em>ob/ob</em> mice showed lower glutamine levels in plasma and tissue, as well as increased insulin resistance and fat in the liver. Conversely, chronic DIP supplementation restored glutamine levels in plasma and tissues, improved glucose homeostasis and reduced plasma and liver lipid levels. Also, Nrf2 restoration, reduced NFkB translocation, and lower TNF-α gene expression was observed in the DIP group. Interestingly, chronic free GLN only increased muscle glutamine stores but reduced overall insulin resistance, and attenuated plasma and liver lipid metabolic biomarkers. The results presented herein indicate that restoration of body glutamine levels reduces oxidative stress and inflammation in obese and T2DM <em>ob/ob</em> mice. This effect attenuated hepatic lipid metabolic changes observed in obesity.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"138 ","pages":"Article 109842"},"PeriodicalIF":4.8,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143007026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypothyroidism induced by excessive-iodine is associated in humans with altered hsa-miR-199a-5p/HIF-1α axis and thyroglobulin","authors":"Jing He ,&nbsp;Yishan Dong ,&nbsp;Xianglan Chen ,&nbsp;Shuo Wang ,&nbsp;Zheng Shen ,&nbsp;Xu Huang ,&nbsp;Weijia Li ,&nbsp;Zhihan Yang ,&nbsp;Jin Cheng ,&nbsp;Jinyu Li ,&nbsp;Qiaoling Liu ,&nbsp;Ziqi Xu ,&nbsp;Dianjun Sun ,&nbsp;Wei Zhang","doi":"10.1016/j.jnutbio.2025.109841","DOIUrl":"10.1016/j.jnutbio.2025.109841","url":null,"abstract":"<div><div>The adverse effect of excessive iodine intake has attracted extensive attention. However, the role of excessive iodine on hypothyroidism and detailed mechanism are not exactly known. Studies have shown that miRNAs are crucial to the occurrence and development of hypothyroidism. Nevertheless, there still limited population-based studies on the miRNA-mRNA regulation in the occurrence of hypothyroidism induced by excessive iodine. Total of 291 hypothyroidism patients and 291 controls matched by sex (1:1) and age (±3 years) were enrolled from Heze City, Shandong Province. Multiple logistic regression analysis revealed that water iodine concentration of 100–300 µg/L was an independent risk factor for hypothyroidism. Additionally, excessive water iodine was associated with an increase in thyroglobulin (Tg) concentration in new diagnosed hypothyroidism patients. Further, high-throughput miRNA sequencing indicated that hsa-miR-19b-3p, hsa-miR-199a-5p, hsa-miR-204-5p and hsa-miR-144-3p were significantly correlated with the occurrence of hypothyroidism. Q-PCR results showed that levels of hsa-miR-199a-5p and hsa-miR-204-5p in the hypothyroidism group were markedly lower than those in the control group. In addition, among the hypothyroidism patients, hsa-miR-199a-5p level in water iodine &gt;100 µg/L group was remarkably higher than that in 10-100 µg/L group. Furthermore, HIF-1α and PD-L1 mRNA levels in whole blood were determined, which are the target genes regulated by miRNA-199a-5p in previous studies. Compared with the control group, HIF-1α mRNA level was significantly increased in the hypothyroidism group. In the hypothyroidism case group, compared with the 10–100 µg/L group, HIF-1α mRNA level was remarkably decreased in water iodine &gt;100 µg/L group. Collectively, miR-199a-5p/HIF-1α axis may contribute to hypothyroidism induced by excessive iodine through thyroglobulin.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"138 ","pages":"Article 109841"},"PeriodicalIF":4.8,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The supply of branched-chain amino acids and branched-chain keto acids alter lipid metabolism, oxidative stress, and apoptosis in primary bovine hepatocytes","authors":"Jayasimha R. Daddam ,&nbsp;Mounica Sura ,&nbsp;Daniel Vocelle ,&nbsp;Juliana G. Laguna ,&nbsp;Kristen Gallagher ,&nbsp;Zheng Zhou","doi":"10.1016/j.jnutbio.2025.109839","DOIUrl":"10.1016/j.jnutbio.2025.109839","url":null,"abstract":"<div><div>Fatty liver impairs liver function and reduces productivity in dairy cows. Our previous <em>in vivo</em> findings demonstrated that branched-chain amino acids (<strong>BCAA</strong>) or branched-chain ketoacid (<strong>BCKA</strong>) improved liver function and lactation performance in dairy cows; however, the underlying mechanisms remain unclear. This study aimed to assess the impact of BCAA or BCKA supplementation on intracellular triglyceride (TG) accumulation, lipid metabolism, antioxidant response, and apoptosis in hepatocytes. Treatments were: control (<strong>CON</strong>): customized medium with amino acids, volatile fatty acids, fatty acids (FA), glucose, choline, insulin, and albumin concentrations equal to circulating levels in cows 4d postpartum; <strong>BCAA:</strong> CON + 33% additional BCAA of plasma BCAA 4d postpartum; and <strong>BCKA:</strong> CON + 33% additional BCKA of plasma BCAA 4d postpartum. Compared to CON, BCAA and BCKA reduced intracellular TG concentration by 32% and 40%, respectively, after 72h. BCAA and BCKA enhanced the uptake of palmitic acid, but upregulated the expression of genes regulating FA oxidation. Although mitochondrial membrane potential was reduced, oxidative protein damage (protein carbonyl levels) was decreased in BCAA- and BCKA-treated hepatocytes without changes in mitochondrial copy number. Additionally, compared to CON, BCAA and BCKA decreased the expression of executioner caspases (<em>caspase 3</em> and <em>caspase 7</em>) and reduced the portion of hepatocytes with activated caspase 3/7, suggesting reduced apoptosis. These findings suggest that BCAA or BCKA supplementation improves hepatocyte lipid metabolism, antioxidant defenses, and apoptosis regulation, potentially mitigating the adverse effects of fatty liver. These mechanisms likely underlie the previously observed improvements in liver function and lactation performance.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"137 ","pages":"Article 109839"},"PeriodicalIF":4.8,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of reduced placental expression of zinc transporters on selective intrauterine growth restriction in monochorionic diamniotic twin pregnancies","authors":"Tingting Xu ,&nbsp;Yao Kong ,&nbsp;Qing Hu ,&nbsp;Hua Liao ,&nbsp;Chunyan Deng ,&nbsp;Xiaodong Wang ,&nbsp;Haiyan Yu","doi":"10.1016/j.jnutbio.2025.109840","DOIUrl":"10.1016/j.jnutbio.2025.109840","url":null,"abstract":"<div><div>Zinc is an essential trace element. The regulatory mechanism of zinc and its transporters in fetal growth in monochorionic diamniotic (MCDA) twins with selective intrauterine growth restriction (MCDA-sIUGR) is unclear. A total of 45 MCDA twins were divided into two groups, MCDA (<em>n</em>=37) and MCDA-sIUGR (<em>n</em>=8), to investigate their possible effects on fetal growth. Maternal and fetal serum zinc levels were measured using a microassay. Immunohistochemistry and western blotting were performed to quantify the expression and localization of zinc transporters. Correlation scatter plots and matrices were used to test the correlation between maternal and cord serum zinc levels, placental zinc transporters, and fetal growth. There was a significant difference in fetal birth weight and placental weight among MCDA T_H (higher birth weight of twin), MCDA T_L (lower birth weight of twin), MCDA-sIUGR T_H, and MCDA-sIUGR T_L groups (<em>P</em> &lt; .05), among which the MCDA-sIUGR T_L group was found to be significantly lower than the other three groups (<em>P</em> &lt; .05). Placental zinc transporters, including Zip 2, metal regulatory transcription factor 1 (MTF 1), and ZnT1, were significantly decreased in the MCDA-sIUGR T_L group compared to those in the other three groups (<em>P</em> &lt; .05). Furthermore, we observed varying degrees of correlation between fetal weight and maternal/fetal serum zinc levels and placental zinc transporters. Our study indicates that placental zinc transporters are impaired in MCDA-sIUGR, particularly in lightweight twins. Therefore, we hypothesized that the reduced placental expression of zinc transporters may affect fetal growth in MCDA twins.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"137 ","pages":"Article 109840"},"PeriodicalIF":4.8,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}