Journal of Oral Microbiology最新文献

{"title":"<i>Candida albicans</i> impacts carbohydrate metabolism of <i>Enterococcus faecalis</i> in interkingdom biofilms.","authors":"Nicole de Mello Fiallos, Iriana J Zanin Dos Santos, Debra N Brunson, Jessica K Kajfasz, Lin Zeng, Rossana de Aguiar Cordeiro, José A Lemos, Jacqueline Abranches","doi":"10.1080/20002297.2025.2492194","DOIUrl":"https://doi.org/10.1080/20002297.2025.2492194","url":null,"abstract":"<p><strong>Objectives: </strong>This study investigated the transcriptional and physiological responses of <i>Enterococcus faecalis</i>, an opportunistic pathogen linked to endodontic infections, when cultivated in dual-species biofilms with <i>Candida albicans</i>, a yeast pathobiont found in the oral cavity.</p><p><strong>Methods: </strong>Forty-eight-hour <i>E. faecalis</i> OG1RF biofilms were developed in BHI medium as mono- or dual-species with <i>C. albicans</i> SC5314. Biofilms were assessed for biomass, colony-forming units (CFUs), and architecture using confocal microscopy. RNA sequencing was performed on an Illumina platform. Mannose-PTS activity and glycerol quantification assays were conducted to investigate changes in carbohydrate metabolism.</p><p><strong>Results: </strong>Transcriptomic analysis revealed 149 <i>E. faecalis</i> genes differentially expressed in dual-species biofilms. Genes linked to mannose-PTS and glycerol metabolism were notably upregulated. Mannose-PTS activity was significantly higher in dual-species biofilms. Mannose, as the sole carbohydrate source, increased <i>E. faecalis</i> CFUs and decreased <i>C. albicans</i> CFUs in co-culture, while glucose had no effect. As <i>C. albicans</i> is a glycerol net producer, glycerol levels were always higher when <i>C. albicans</i> was present, likely contributing to the upregulation of glycerol metabolism genes in <i>E. faecalis</i> when in co-cultures.</p><p><strong>Conclusions: </strong>The presence of <i>C. albicans</i> alters <i>E. faecalis</i> gene expression and metabolism, suggesting metabolic crosstalk that may influence their pathogenicity and role in oral infections.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2492194"},"PeriodicalIF":3.7,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12004723/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144012620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary analysis of salivary microbiota in catathrenia (nocturnal groaning) using machine learning algorithms.","authors":"Min Yu, Yujia Lu, Wanxin Zhang, Xu Gong, Zeliang Hao, Liyue Xu, Yongfei Wen, Xiaosong Dong, Fang Han, Xuemei Gao","doi":"10.1080/20002297.2025.2489613","DOIUrl":"https://doi.org/10.1080/20002297.2025.2489613","url":null,"abstract":"<p><strong>Objectives: </strong>The present study aimed to characterize the salivary microbiota in patients with catathrenia and to longitudinally validate potential biomarkers after treatment with mandibular advancement devices (MAD).</p><p><strong>Materials and methods: </strong>Twenty-two patients with catathrenia (12 M/10 F, median age 28 y) and 22 age-matched control volunteers (8 M/14 F, median age 30 y) were included in the cross-sectional study. Video/audio polysomnography was conducted for diagnosis. All patients received treatment with custom-fit MAD and were followed for one month. Ten patients (6 M/4 F) underwent post-treatment PSG. Salivary samples were collected, and microbial characteristics were analyzed using 16S rRNA gene sequencing. The 10-fold cross-validated XGBoost and nested Random Forest Classifier machine learning algorithms were utilized to identify potential biomarkers.</p><p><strong>Results: </strong>In the cross-sectional study, patients with catathrenia had lower α-diversity represented by Chao 1, Faith's phylogenetic diversity (pd), and observed species. Beta-diversity based on the Bray-Curtis dissimilarities revealed a significant inter-group separation (<i>p</i> = 0.001). The inter-group microbiota distribution was significantly different on the phylum and family levels. The treatment of MAD did not alter salivary microbiota distribution significantly. Among the most important genera in catathrenia and control classification identified by machine learning algorithms, four genera, <i>Alloprevotella, Peptostreptococcaceae_XI_G1, Actinomyces</i> and <i>Rothia</i>, changed significantly with MAD treatment. Correlation analysis revealed that <i>Alloprevotella</i> was negatively related to the severity of catathrenia (r²= -0.63, <i>p</i> < 0.001).</p><p><strong>Conclusions: </strong>High-throughput sequencing revealed that the salivary microbiota composition was significantly altered in patients with catathrenia. Some characteristic genera (<i>Alloprevotella, Peptostreptococcaceae_XI_G1, Actinomyces,</i> and <i>Rothia</i>) could be potential biomarkers sensitive to treatment. Future studies are needed to confirm and determine the mechanisms underlying these findings.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2489613"},"PeriodicalIF":3.7,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12004722/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Odontogenic brain abscess caused by <i>Porphyromonas gingivalis</i> and <i>Streptococcus constellatus</i>: a case report and review article.","authors":"Siyu Sun, Rui He, Shan Chen, Jing Ren, Xinrong Ma, Junying Yang","doi":"10.1080/20002297.2025.2485197","DOIUrl":"https://doi.org/10.1080/20002297.2025.2485197","url":null,"abstract":"<p><strong>Background: </strong>Odontogenic brain abscess is a rare, but potentially fatal, central nervous system infection, with insidious onset and unclear etiology.</p><p><strong>Methods: </strong>This case reports a 70-year-old male patient who developed an odontogenic brain abscess secondary to periodontal infection and underwent neurological surgery. Extract pus during surgery for the metagenomic next-generation sequencing (mNGS).</p><p><strong>Results: </strong>The mNGS of pus samples obtained from brain abscess aspiration identified the periodontal pathogens <i>Porphyromonas gingivalis</i> and <i>Streptococcus constellatus</i>. Consequently, he was referred to the department of stomatology for further examination and treatment.</p><p><strong>Conclusions: </strong>Our study found that major periodontal pathogens including <i>P. gingivalis</i> and <i>S. constellatus</i> were essential in the development of odontogenic brain abscesses; thus, timely intervention and preventive measures are important for treatment.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2485197"},"PeriodicalIF":3.7,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12001842/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144000152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interactions between non-<i>albicans Candida</i> and <i>Streptococcus mutans</i> enhance cariogenic potential of dual-species interkingdom biofilms.","authors":"Wirunphat Sansanoa, Oranart Matangkasombut, Metanee Sangpitak, Lakshman Samaranayake, Panida Thanyasrisung","doi":"10.1080/20002297.2025.2492198","DOIUrl":"https://doi.org/10.1080/20002297.2025.2492198","url":null,"abstract":"<p><strong>Background: </strong>Non-<i>albicans Candida</i> species (NACs) are commonly found in carious lesions, yet their specific role in caries progression remains unclear. Hence, we conducted an <i>in</i> <i>vitro</i> study to explore how NACs interactions with <i>Streptococcus mutans</i> affect cariogenicity.</p><p><strong>Materials and methods: </strong>Dual-species interkingdom biofilms were developed with <i>S.mutans</i> and six <i>Candida</i> species, <i>C.albicans</i> and the NACs: <i>C.dubliniensis</i>, <i>C.glabrata</i>, <i>C.krusei</i>, <i>C.parapsilosis</i> and <i>C.tropicalis</i>. Biofilm mass, viable cell counts, and pH were evaluated in mono- and dual-species biofilms. Quantitative RT-PCR was used to assess the expression of <i>S.mutans</i> genes associated with cariogenicity.</p><p><strong>Results: </strong>Co-culturing <i>S.mutans</i> with either <i>C.albicans</i>, <i>C.glabrata</i>, or <i>C.tropicalis</i> significantly increased biofilm mass. While <i>S.mutans</i> numbers either increased or remained stable in dual-species biofilms, <i>C.krusei</i>, <i>C.parapsilosis</i>, and in particular <i>C.tropicalis</i> numbers significantly increased. All dual-species biofilms exhibited a pH below the critical demineralization level of enamel, akin to <i>S.mutans</i> mono-species biofilms. The expression of a battery of cariogenic genes in <i>S.mutans</i> was upregulated, particularly in dual-species biofilms with <i>C.krusei</i> and with <i>C.tropicalis</i>.</p><p><strong>Conclusion: </strong>NACs influence the biofilm production and the cariogenic gene expression of <i>S.mutans.</i> The dual-species biofilm of <i>S.mutans</i> and NACs, particularly <i>C.tropicalis</i>, likely possess heightened cariogenic potential. Further research is warranted to unravel these intriguing interactions within interkingdom biofilms.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2492198"},"PeriodicalIF":3.7,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11998306/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144002519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitrate reduction by salivary bacteria, glucose metabolism, and lifestyle.","authors":"Evangelia Morou-Bermúdez, Kai Guo, Jairelisse Morales Morales, Karina Ricart, Rakesh P Patel, José C Clemente, Kaumudi Joshipura","doi":"10.1080/20002297.2025.2489612","DOIUrl":"10.1080/20002297.2025.2489612","url":null,"abstract":"<p><strong>Background: </strong>Nitrate reductases (NR) expressed in oral bacteria reduce nitrate to nitrite. Depending on the environmental conditions and types of bacteria present nitrite can be further reduced to ammonium via Dissimilatory Nitrate Reduction to Ammonium (DNRA), or alternatively to nitric oxide (NO), which impacts cardiometabolic health.</p><p><strong>Objective: </strong>To evaluate the associations between nitrate reduction by salivary bacteria, clinical markers of glucose metabolism, and lifestyle factors that can modulate the oral environment, potentially impacting DNRA and NR expression.</p><p><strong>Methods: </strong>A cross-sectional study was conducted using a convenience sample of 144 participants from the San Juan Overweight Adult Longitudinal Study (SOALS), which includes data on glucose metabolism and lifestyle. DNRA and NR activities were measured in saliva under aerobic or CO₂-enriched conditions.</p><p><strong>Results: </strong>DNRA activity was inversely associated with insulin resistance (HOMA-IR) [aerobic_{3rd vs.1st tertile}: β=-0.48 (-0.81, -0.15); CO₂-enriched_{3rd vs.1st tertile} β=-0.42 (-0.68, -0.17)], fasting blood glucose [aerobic_{3rd vs.1st tertile} β=-0.144 (-0.268, -0.019); CO₂-enriched_{3rd vs.1st tertile}: β=-0.070 (-0.130, -0.011)], and 2-h glucose [CO₂-enriched_{3rd vs.1st tertile}β=-0.21 (-0.37, -0.04)]. Current smokers had lower DNRA activity than non-smokers under aerobic conditions [β=-1.55 (-2.96, -0.14)], but higher under CO₂-enriched conditions [β = 0.93 (0.15, 1.71)]. Toothbrushing frequency (twice/day vs. once/day) was positively associated with DNRA activity under CO₂-enriched conditions [β = 4.11 (1.90, 6.32)] and with aerobic NR activity [β = 1.20, (0.14, 2.27)]. Physical activity was inversely associated with aerobic NR [β=-0.01, (-0.022, -0.003)]. Under CO₂-enriched conditions NR was inversely associated with the BMI (β=-0.11, <i>p</i> = 0.007). Aerobic NR was higher when sucrose was added to the assays (NADP vs. sucrose β=-0.74, <i>p</i> = 0.02) and positively associated with salivary nitrate levels (β = 0.002, <i>p</i> = 0.002).</p><p><strong>Conclusions: </strong>Nitrate reduction by salivary bacteria is inversely associated with insulin resistance and can be modulated by lifestyle factors. This knowledge could lead to the development of novel, non-invasive approaches for monitoring and preventing diabetes progression.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2489612"},"PeriodicalIF":3.7,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11986870/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling salivary microbiota diversity following kidney transplantation: insights from baseline peripheral blood lymphocyte subsets.","authors":"Xuyu Xiang, Tianyin Wang, Peng Ding, Yi Zhu, Ke Cheng, Yingzi Ming","doi":"10.1080/20002297.2025.2490284","DOIUrl":"https://doi.org/10.1080/20002297.2025.2490284","url":null,"abstract":"<p><strong>Background: </strong>Effective biomarkers are urgently needed to monitor immune suppression in kidney transplantation (KT) recipients. Our study identified a close association between the salivary microbiota and immunosuppressant concentrations. It is therefore hypothesized that the salivary microbiota may be linked to immune function.</p><p><strong>Materials and methods: </strong>We analyzed 108 saliva samples from 37 KT patients using 16S rRNA sequencing. Patients were clustered via K-means based on peripheral blood lymphocyte subset (PBLS) counts.</p><p><strong>Results: </strong>Cluster1 exhibited significantly higher CD4⁺ T cells (<i>p</i> < 0.0001), CD8⁺ T cells (<i>p</i> < 0.0001), and B cells (<i>p</i> = 0.0071) versus Cluster2, with marginally NK cells (<i>p</i> = 0.2319). Beta diversity indicated significant differences in microbial communities. LEfSe analysis identified 34 differential taxa at the genus level. A random forest model in a fivefold three-times repeated cross-validation, developed with differential taxa, discriminated patient groups well (AUC, 75.61% ± 14.54%), with Pseudopropionibacterium most contributing. Meanwhile, only Pseudopropionibacterium correlated with more than 2 PBLSs. Cluster2 was predicted to exhibit more primary and secondary bile acid synthesis, with differential expression of related enzymes.</p><p><strong>Conclusion: </strong>The absolute count of PBLSs is correlated with the composition of the salivary microbiota, with the strongest association observed between Pseudopropionibacterium and lymphocytes. Our study provides novel insights into immune monitoring post-KT.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2490284"},"PeriodicalIF":3.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11983535/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glycosylation of oral bacteria in modulating adhesion and biofilm formation.","authors":"Simeng Yi, Yingyu Liu, Qinrui Wu, Danning Zhao, Zhengyi Li, Xian Peng, Ga Liao, Shida Wang","doi":"10.1080/20002297.2025.2486650","DOIUrl":"https://doi.org/10.1080/20002297.2025.2486650","url":null,"abstract":"<p><strong>Background: </strong>Glycosylation is a ubiquitous biochemical process that covalently attaches glycans to proteins or lipids, which plays a pivotal role in modulating the structure and function of these biomolecules.  This post-translational modification is prevalent in living organisms and intricately regulates various biological processes, including signaling transduction, recognition, and immune responses.  In the oral environment, bacteria ingeniously use glycosylation to enhance their adhesion to oral surfaces, which is a key step in biofilm formation and subsequent development.  This adhesion process is intimately associated with the onset and progression of oral diseases, including dental caries and periodontal disease.</p><p><strong>Objective: </strong>This review aims to describe the types and mechanisms of glycosylation in oral bacteria, and to understand the role of glycosylation in the adhesion, biofilm formation and virulence of oral bacteria.</p><p><strong>Methods: </strong>We reviewed articles on glycosylation in a variety of oral bacteria.</p><p><strong>Conclusion: </strong>In cariogenic bacteria and periodontopathic pathogens, glycosylation facilitates adhesion and subsequent biofilm maturation on tooth surface.   Distinct glycosylation patterns in oral bacteria shape biofilm structure and function, influencing microbial interactions and community stability.   Pathogen-specific glycosylation signatures enhance virulence and ecological competitiveness, contributing to disease progression. Glycosylation plays a critical role in bacterial virulence and community  interactions, with significant implications for oral health and disease development.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2486650"},"PeriodicalIF":3.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11983588/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of immunostimulatory deoxycytidylate-phosphate-deoxyguanylate (CpG) motifs in oral bacteria associated with oral diseases.","authors":"Pisit Charoenwongwatthana, Oslovenya S Caroline, Halah Ahmed, Jamie Coulter, Chien-Yi Chang","doi":"10.1080/20002297.2025.2486639","DOIUrl":"https://doi.org/10.1080/20002297.2025.2486639","url":null,"abstract":"<p><strong>Background: </strong>CpG oligodeoxynucleotide motifs in bacterial DNA with composition variations exhibit potent immunostimulation. The effect of different compositions in oral infections is unclear. This study aims to investigate CpG motifs in bacteria associated with endodontic diseases, periodontal diseases, and dental caries to elucidate their influence on host immune response.</p><p><strong>Methods: </strong>Fifty oral bacterial genomes were selected for <i>in silico</i> analysis to determine GC% content and CpG motif frequency in each genome. The relationships between GC% content, CpG motif frequency, and genome size were assessed using correlation analysis. Normalisation of immunostimulatory sequences was implemented to enable unbiased comparison of frequency counts among bacteria.</p><p><strong>Results: </strong>Sixty percent of bacteria exhibited medium GC% content (<i>Mdn</i> = 44), with no significant difference among bacteria associated with these diseases (<i>p</i> = 0.66). A positive correlation between GC% content and CpG motif frequency, as well as genome size and CpG motifs frequency was observed. A higher-than-mean of the human immunostimulatory motif (GTCGTT, 7/14) and the mice/rabbits immunostimulatory motif (GACGTT, 9/14) was observed in core endodontic microbiota.</p><p><strong>Conclusion: </strong>CpG motifs in oral bacteria might drive disease progression through host immunomodulation. Variation in bacterial CpG motifs suggests targeting these motifs offers a promising therapeutic intervention.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2486639"},"PeriodicalIF":3.7,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11983586/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144012269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential regulatory role of mannose phosphotransferase system EII in alkaline resistance of <i>Enterococcus faecalis</i>.","authors":"Runze Liu, Yi Luo, Pei Liu, Shuo Xiao, Wei Fan, Bing Fan","doi":"10.1080/20002297.2025.2487944","DOIUrl":"https://doi.org/10.1080/20002297.2025.2487944","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the role of mannose phosphotransferase system (Man-PTS) EII in the alkaline resistance of <i>Enterococcus faecalis</i> (<i>E. faecalis</i>).</p><p><strong>Methods: </strong>The Man-PTS EIID (<i>mptD</i>) deletion (Δ<i>mptD</i>) and overexpressing (+<i>mptD</i>) strains of <i>E. faecalis</i> were constructed. The contribution of the Man-PTS EII to alkaline resistance was investigated by assessing growth and biofilm formation of <i>E. faecalis</i> at pH 7 and 10, as well as changes in intracellular ATP levels, pH, potassium ion concentration, membrane potential and membrane permeability. The effects of <i>mptD</i> on the expression of other Man-PTS EII components and membrane transport-related genes were evaluated by RT-qPCR at pH 10. In addition, six clinical strains were isolated. Three strains of <i>E. faecalis</i> with high to low alkaline resistance were screened, and RNA sequencing was performed to further explore the role of Man-PTS EII in the alkaline resistance.</p><p><strong>Results: </strong><i>mptD</i> enhanced the alkaline resistance of <i>E. faecalis</i> by maintaining pH homeostasis in an alkaline environment. Overexpression of <i>mptD</i> induced membrane hyperpolarization, reduced membrane permeability, decreased intracellular K⁺ levels, lowered cytoplasmic pH, and elevated ATP production, while the loss of <i>mptD</i> reversed these effects (<i>p</i> < 0.05). <i>mptD</i> coordinated the expression of Man-PTS EII components and upregulated genes encoding membrane transporter components (<i>p</i> < 0.01). All Man-PTS EII components were positively related to the degree of alkaline resistance of <i>E. faecalis</i> (<i>p</i> < 0.05).</p><p><strong>Conclusion: </strong>Man-PTS EII is a key factor in the interaction between metabolism and ion membrane transport during the alkaline resistance process of <i>E. faecalis</i>. This study may provide new insights for understanding the alkaline resistance of <i>E. faecalis</i>.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2487944"},"PeriodicalIF":3.7,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11980212/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144007569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tongue coating microbiota-based machine learning for diagnosing digestive system tumours.","authors":"Yubo Ma, Zhengchen Jiang, Yanan Wang, Libin Pan, Kang Liu, Ruihong Xia, Li Yuan, Xiangdong Cheng","doi":"10.1080/20002297.2025.2487645","DOIUrl":"https://doi.org/10.1080/20002297.2025.2487645","url":null,"abstract":"<p><strong>Background: </strong>Digestive system tumours (DSTs) often diagnosed late due to nonspecific symptoms. Non-invasive biomarkers are crucial for early detection and improved outcomes.</p><p><strong>Patients and methods: </strong>We collected tongue coating samples from 710 patients diagnosed with DST and 489 healthy controls (HC) from April 2023, to December 2023. Microbial composition was analyzed using 16S rRNA sequencing, and five machine learning algorithms were applied to assess the diagnostic potential of tongue coating microbiota.</p><p><strong>Results: </strong>Alpha diversity analysis showed that the microbial diversity in the tongue coating was significantly increased in DST patients. LEfSe analysis identified DST-enriched genera Alloprevotella and Prevotella, contrasting with HC-dominant taxa Neisseria, Haemophilus, and Porphyromonas (LDA >4). Notably, when comparing each of the four DST subtypes with the HC group, the proportion of Haemophilus in the HC group was significantly higher, and it was identified as an important feature for distinguishing the HC group. Machine learning validation demonstrated superior diagnostic performance of the Extreme Gradient Boosting (XGBoost) model, achieving an AUC of 0.926 (95% CI: 0.893-0.958) in internal validation, outperforming the other four machine learning models.</p><p><strong>Conclusion: </strong>Tongue coating microbiota shows promise as a non-invasive biomarker for DST diagnosis, supported by robust machine learning models.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2487645"},"PeriodicalIF":3.7,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11980229/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144030772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}