Journal of Oral Microbiology最新文献

{"title":"Supragingival plaque microbiome features in allergic rhinitis and allergic asthma and their association with dental caries: a cross-sectional study.","authors":"Qi Yu, Yang Zheng, Yachun Li, Shengjie Li, Wenzhi Wu, Lei Lan, Jiajie Mao, Kaiwen Fang, Zhuo Chen, Ya-Dong Gao","doi":"10.1080/20002297.2026.2732753","DOIUrl":"10.1080/20002297.2026.2732753","url":null,"abstract":"<p><strong>Background: </strong>Allergic rhinitis (AR) and allergic asthma (AS) are associated with increased risk of dental caries, potentially associated with alterations in the oral microbiome.</p><p><strong>Objective: </strong>To characterize supragingival plaque microbiome features and their associations with caries severity in allergic adults.</p><p><strong>Design: </strong>This cross-sectional study enrolled patients with cat dander-induced AR or AS, along with age- and sex-comparable normal controls (NC). Supragingival plaque was collected for 16S rRNA gene sequencing. Microbial diversity, ANCOM-BC2 with multiple-testing correction and pseudo-count sensitivity analysis and LEfSe exploratory analysis were performed. Factors of caries severity (DMFT/DMFS) were examined with a random forest model and multivariable negative binomial regression.</p><p><strong>Results: </strong>A total of 89 participants (32 AR, 46 AS and 11 NC) were included. Allergic patients exhibited significantly higher <i>α</i>-diversity than NC. ANCOM-BC2 analysis identified 24 taxa with an omnibus FDR threshold; a family-resolved, genus-unclassified <i>Prevotellaceae</i> passed the pseudo-count sensitivity analysis. LEfSe analysis identified <i>Treponema</i> and <i>Bacteroidota</i> as AR-enriched taxa, and <i>Actinobacteriota</i> as AS-enriched taxa. <i>Treponema</i> was the top-ranked random forest feature. Among allergic patients, regular dental check-ups were associated with lower DMFT (IRR = 0.47, 95% CI: 0.30-0.75) and DMFS (IRR = 0.34, 95% CI: 0.17-0.69). <i>Corynebacterium</i> abundance was inversely associated with DMFT (IRR = 0.56, 95% CI: 0.31-0.98), and <i>Actinomyces</i> abundance was positively associated with DMFS (IRR = 2.96, 95% CI: 1.07-8.19).</p><p><strong>Conclusions: </strong>Plaque microbial communities differed across groups, and selected taxa were associated with caries severity among allergic adults. Future investigations are needed to evaluate temporality and mechanisms.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2732753"},"PeriodicalIF":6.7,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13587540/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149008415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laminin functionalization of zirconia abutments modulates early oral biofilm formation in vitro.","authors":"Romualdus Nugraha Catur Utomo, Alena L Palkowitz, Philipp Schräder, Lin Gan, Hendrik Ballerstedt, Martin Zimmermann, Lars M Blank, Horst Fischer, Stefan Wolfart, Taşkın Tuna","doi":"10.1080/20002297.2026.2731523","DOIUrl":"https://doi.org/10.1080/20002297.2026.2731523","url":null,"abstract":"<p><strong>Background: </strong>Surface properties of dental implant abutment materials critically influence supragingival biofilm formation by determining the outcome of the early 'race for the surface', in which gingival epithelial cells and fibroblasts compete with bacteria for initial adhesion. This study hypothesized that biofunctionalization with extracellular matrix (ECM) proteins fibronectin (FN) or laminin (LN) modulates early oral biofilm formation on titanium alloy (Ti6Al4V) and yttria-stabilized zirconia (Y-TZP) abutments.</p><p><strong>Methods: </strong>Saliva-derived microcosm biofilms were grown on control and biofunctionalized discs. Biofilm biomass, viability, structure, microbial community composition and metabolic activity were assessed using microscopy-based methods, impedance flow cytometry, 16S rRNA sequencing and short-chain fatty acid analysis.</p><p><strong>Results: </strong>Laminin-functionalized Y-TZP showed the most pronounced delay in early biofilm formation. Biofilm accumulation was delayed during the first three days and was associated with the lowest number of viable cells on day 4, minimal extracellular polymeric substance formation, reduced <i>Veillonella</i> abundance (~9% on day 1) and decreased short-chain fatty acid production.</p><p><strong>Conclusion: </strong>ECM-based coatings do not exert bactericidal effects but transiently reshape early oral biofilm formation. Laminin-functionalized zirconia may act as a biologically selective interface that favors beneficial early host-microbe interactions, potentially creating a microbial environment more compatible with peri-implant soft-tissue health.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2731523"},"PeriodicalIF":6.7,"publicationDate":"2026-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13580371/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148990326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anaerobic stress index to predict microbial ecological shifts in clear aligners.","authors":"Mehmet Demirci, Ozge Unlu, Alpdogan Kantarci","doi":"10.1080/20002297.2026.2701561","DOIUrl":"https://doi.org/10.1080/20002297.2026.2701561","url":null,"abstract":"<p><strong>Objectives: </strong>Clear aligners are clinically perceived as periodontally 'friendlier' than fixed appliances (FA), yet localized gingivitis persists. Salivary diagnostics often mask site-specific dysbiosis through a 'dilution effect'. This study aimed to characterize distinct ecological pressures and propose a novel anaerobic stress index (ASI) to quantify localized microbial burden.</p><p><strong>Methods: </strong>A computational meta-analysis was conducted using longitudinal 16S rRNA sequencing datasets (<i>n</i> = 976 samples from 10 distinct studies). Data was processed through a standardized Kraken2/Bracken pipeline. The ASI was calculated as the ratio of key anaerobic pathobionts (<i>Fusobacterium, Prevotella</i>) to facultative commensals (<i>Streptococcus, Rothia</i>).</p><p><strong>Results: </strong>Saliva acts as a 'dilution filter,' failing to capture sessile biofilm dynamics (AUC = 0.51 in FA). Fixed appliances exhibited mechanical dysbiosis with a 2.64-fold increase in <i>Actinomyces</i> (<i>p</i> < 0.0001) within the first month. Clear aligners induced a 'silent hypoxia'. The supragingival biofilm showed a progressive shift toward anaerobiosis. The ASI in aligner patients quadrupled by month 6 (from 0.58 to 2.52; <i>p</i> = 0.002), a shift not detectable in matched saliva samples.</p><p><strong>Conclusions: </strong>Physical entrapment in fixed appliances versus micro-environmental hypoxia under clear aligners drives divergent dysbiotic pathways. The ASI serves as a robust biomarker for subclinical dysbiosis, suggesting periodic re-oxygenation therapeutics are necessary to restore host-microbe homeostasis.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2701561"},"PeriodicalIF":6.7,"publicationDate":"2026-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13580376/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148990350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harmonized multi-cohort analysis of oral microbiome development during early lifetime.","authors":"Do Yeon Won, Si Yeong Kim, Seon Mi Kim, Hyewon Shin, Hee Sam Na, Jin Chung","doi":"10.1080/20002297.2026.2728748","DOIUrl":"10.1080/20002297.2026.2728748","url":null,"abstract":"<p><strong>Background: </strong>Early-life assembly of the oral microbiome may influence oral and systemic health, yet developmental patterns are difficult to compare across studies because of differences in sampling and analytical workflows. We conducted a harmonized cross-cohort re-analysis of longitudinal pediatric 16S rRNA datasets spanning birth to early childhood.</p><p><strong>Methods: </strong>Five publicly available longitudinal cohorts from birth to seven years were curated and independently reprocessed using QIIME 2 and R. Taxonomy was assigned using the Human Oral Microbiome Database (HOMD). Alpha and beta diversity, taxonomic composition, and species-level temporal dynamics were evaluated. Age was harmonized to months and summarized into three developmental intervals: birth-12 months, 12-36 months, and >36 months. Temporal clustering used fuzzy c-means clustering (TCseq).</p><p><strong>Results: </strong>Alpha diversity increased rapidly during the first year, plateaued between one and three years, and increased gradually thereafter. Beta diversity showed the greatest compositional shifts during infancy, followed by relative stabilization. Taxonomic profiling revealed reproducible succession from <i>Streptococcus</i> and <i>Staphylococcus</i> to <i>Haemophilus</i>, <i>Neisseria</i>, and <i>Veillonella</i>, followed by <i>Prevotella</i> and <i>Campylobacter</i> after three years. Temporal clustering identified three developmental groups corresponding to early, transitional, and later-expanding taxa.</p><p><strong>Conclusions: </strong>This harmonized analysis of five longitudinal cohorts demonstrates a reproducible three-phase trajectory of oral microbiome maturation during early childhood. The most pronounced microbial restructuring occurred during infancy, followed by community stabilization and gradual maturation. These findings provide a framework for understanding age-associated microbial succession and identifying developmental windows for preventive interventions in pediatric oral health.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2728748"},"PeriodicalIF":6.7,"publicationDate":"2026-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13573508/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148969164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative inhibition of oral pathogens, biofilm formation, and inflammation-related responses by postbiotics from <i>Lactobacillus kefiranofaciens</i> DD2.","authors":"Eun-Ah Jung, Hyunsook Kim, Wallace H Yokoyama, Nitin Nitin, Kun-Ho Seo","doi":"10.1080/20002297.2026.2712769","DOIUrl":"10.1080/20002297.2026.2712769","url":null,"abstract":"<p><strong>Background: </strong>Postbiotics derived from lactic acid bacteria are attracting significant interest as safe and stable alternatives to live probiotics for controlling oral biofilms and inflammation.</p><p><strong>Objective: </strong>Building on previous research that identified <i>Lactobacillus kefiranofaciens</i> DD2 as a potent anti-cariogenic strain, we compared antimicrobial, anti-biofilm, and anti-inflammatory properties of postbiotics produced by DD2 with those from two newly isolated kefir strains (LKF4 and LK12) and two commercial probiotics, <i>Lactiplantibacillus plantarum</i> ATCC 3105 and <i>Lacticaseibacillus rhamnosus</i> ATCC 7469.</p><p><strong>Materials and methods: </strong>Cell-free postbiotics were evaluated for their antimicrobial and anti-biofilm activities against major oral pathogens, including <i>Streptococcus mutans</i>, <i>Streptococcus sobrinu</i>s, <i>Porphyromonas gingivalis</i>, and <i>Porphyromonas gulae</i>. Furthermore, their immunomodulatory effects were investigated in lipopolysaccharide-stimulated RAW 264.7 macrophages through nitric oxide assays and reverse transcription quantitative polymerase chain reaction.</p><p><strong>Results: </strong>All tested postbiotics significantly inhibited planktonic growth and biofilm formation of the pathogens (<i>p</i> < 0.05), and DD2 consistently demonstrated the strongest suppression across all species. In macrophages, DD2-derived postbiotics markedly reduced the expression of pro-inflammatory mediators (<i>Cox2, Nos2, Tnf-α, and Il-1β</i>) to approximately 0.5-fold relative to the control, while inducing the highest levels of the anti-inflammatory cytokine <i>Il-10</i>. DD2 upregulated <i>Tlr4</i>, <i>Nrf2</i>, and <i>Ho-1</i> mRNA, indicating the preservation of pathogen sensing and the activation of cytoprotective pathways via the Nrf2/Ho-1 signaling axis. Notably, this multi-targeted approach highlighted DD2's superior efficacy compared to other kefir and commercial strains.</p><p><strong>Conclusions: </strong>These findings support the potential of DD2-derived postbiotics as bioactive alternatives for managing oral pathogens, biofilm formation, and inflammation relevant to oral health. Further studies are needed to identify the active components and to validate these effects in oral <i>in vivo</i> models.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2712769"},"PeriodicalIF":6.7,"publicationDate":"2026-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13564168/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148955899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mapping the oral microbiome in children with oral diseases using next-generation sequencing: a systematic review.","authors":"Marah Alsaid, Manikandan Ekambaram, Nicholas C K Heng, Noren Nor Hasmun","doi":"10.1080/20002297.2026.2723644","DOIUrl":"10.1080/20002297.2026.2723644","url":null,"abstract":"<p><strong>Background: </strong>The oral microbiome is integral to paediatric oral health and disease. Next-generation DNA sequencing (NGS) technology enables culture-independent profiling of microbial communities, yet findings remain inconsistent due to methodological and population heterogeneity.</p><p><strong>Objective: </strong>This review synthesised NGS-based evidence comparing the oral microbiome of children/adolescents with oral diseases and healthy controls.</p><p><strong>Design: </strong>The protocol was registered in PROSPERO (CRD42025618407). PubMed, Medline, Embase, Scopus and Web of Science were searched for peer-reviewed studies (2015-2025). Eligible studies used 16S rRNA gene sequencing to compare children/adolescents (0-18 years) with oral diseases and healthy controls. Two reviewers independently screened studies, extracted data and assessed risk of bias using validated tools. Findings were synthesised narratively due to heterogeneity.</p><p><strong>Results: </strong>From 4,767 records, 61 studies were included. Most were cross-sectional designs conducted across diverse populations and conditions. Differences in community composition were more consistent than changes in alpha diversity, which varied by disease stage and sampling site. Shifts toward anaerobic, metabolically active communities were observed. Dental caries was consistently linked to acidogenic genera/species, whereas fluorosis, halitosis and gingivitis showed functional shifts involving proteolytic, sulphur-metabolising and anaerobic bacteria.</p><p><strong>Conclusion: </strong>Paediatric oral diseases are more strongly associated with condition- and niche-specific compositional alterations than with uniform diversity changes.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2723644"},"PeriodicalIF":6.7,"publicationDate":"2026-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13564157/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148955913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From microbial promotion to co-exposure governance: policy implications of <i>Candida albicans</i> in oral carcinogenesis.","authors":"Ardiyansah, Fitrotun Niswah","doi":"10.1080/20002297.2026.2731658","DOIUrl":"10.1080/20002297.2026.2731658","url":null,"abstract":"","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2731658"},"PeriodicalIF":6.7,"publicationDate":"2026-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13564159/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148955942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative meta-analysis of the OSCC microbiome: from niche-specific heterogeneity to universal network and functional signatures.","authors":"Hee Sam Na, Jung Hwa Park, Tae Sung Kim","doi":"10.1080/20002297.2026.2730638","DOIUrl":"10.1080/20002297.2026.2730638","url":null,"abstract":"<p><strong>Background: </strong>While the association between oral squamous cell carcinoma (OSCC) and microbial dysbiosis is well-established, high inter-study heterogeneity has historically hindered the identification of universal biomarkers.</p><p><strong>Objective: </strong>To explore the profound influence of anatomical ecological niches on microbial profiles while identifying shared oncogenic signatures across diverse populations.</p><p><strong>Design: </strong>An integrative meta-analysis of independent OSCC microbiome cohorts was conducted.</p><p><strong>Results: </strong>Our results demonstrate that microbial community composition is predominantly driven by the sampling site rather than the disease state alone, with tissue-derived samples forming a distinct ecological cluster. Despite this site-specific dominance, a consistent core of anaerobic pathogens-including <i>Fusobacterium</i>, <i>Capnocytophaga</i>, and <i>Treponema</i> was significantly enriched in OSCC across multiple datasets. Network-based analysis identified <i>Fusobacterium</i> as top driver of microbial community restructuring. Diagnostically, saliva-based models achieved superior accuracy compared to tissue and mucosal models, suggesting that saliva serves as an integrative reservoir for oral pathological changes. Functional profiling further revealed a significant functional convergence toward pathways essential for rapid bacterial proliferation and environmental adaptation, such as ribosome biogenesis, DNA replication, and flagellar assembly.</p><p><strong>Conclusions: </strong>These findings emphasize the necessity of standardizing sampling protocols and provide a robust scientific basis for non-invasive, saliva-based precision diagnostic strategies for OSCC.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2730638"},"PeriodicalIF":6.7,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13552301/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148912576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Fusobacterium</i> bacteriophage FNU1 disrupts complex periodontal pathogenic biofilms comprising <i>Fusobacterium polymorphum</i>, <i>Porphyromonas gingivalis</i>, <i>Tannerella forsythia</i> and <i>Prevotella intermedia</i>.","authors":"Siti Saleha Binte Mohamed Yakob Adil, Joseph Tucci, Stuart Dashper, Helen Irving, Mwila Kabwe","doi":"10.1080/20002297.2026.2726627","DOIUrl":"10.1080/20002297.2026.2726627","url":null,"abstract":"<p><strong>Background: </strong>Periodontitis is a chronic inflammatory disease affecting more than 50% of the global population, resulting in irreversible gum damage, alveolar bone loss and ultimately tooth loss. Periodontitis arises from stable, multi-species biofilms whose pathogenicity is driven by emergent ecological interactions persistent to antimicrobial perturbations. <i>Fusobacterium</i> spp. are considered key microbial components that provide scaffolding for this ecological niche, which includes the pathobionts <i>Tannerella forsythia</i>, <i>Prevotella intermedia</i> and <i>Porphyromonas gingivalis</i>.</p><p><strong>Objectives: </strong>To examined how oral microbial interactions shape periodontal biofilms and whether selectively targeting <i>Fusobacterium polymorphum</i> by bacteriophages can disrupt this pathogenic community structure.</p><p><strong>Materials and methods: </strong><i>In vitro</i> biofilms were established using (i) <i>F. polymorphum, T. forsythia</i> and <i>P. intermedia</i> on their own, (ii) <i>T. forsythia</i> and <i>P. intermedia</i> in dual-species biofilms with <i>F. polymorphum</i> and (iii) four-species complex biofilms involving <i>F. polymorphum</i>, <i>T. forsythia</i>, <i>P. intermedia</i> and <i>P. gingivalis</i>. The biofilms were then exposed to the <i>F. polymorphum</i>-specific bacteriophage FNU1 to assess the ecological impact of targeted viral predation on a single member of the biofilm community. Biofilm formation, spatial organisation and microbial interactions were visualised by confocal laser scanning microscopy using CellTrace™ proliferation assays and live-dead staining. Community biofilm biomass was quantified using 0.1% crystal violet staining.</p><p><strong>Results: </strong>Multi-species biofilms displayed enhanced biomass and increased cell density relative to simpler communities. Disruption of <i>F. polymorphum</i> by bacteriophage FNU1 led to significant reductions in total biofilm biomass and altered cell density in the single-species <i>F. polymorphum</i> biofilm as well as dual- and four-species complex biofilms.</p><p><strong>Conclusion: </strong>Our findings support a role for <i>F. polymorphum</i>-specific bacteriophages in destabilising complex biofilms of periodontal pathobionts. They highlight the importance of <i>F. polymorphum</i> in community dynamics in microbial biofilms and that its precision targeting results in ecological perturbation with the potential to reverse dysbiosis in chronic inflammation in periodontitis.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2726627"},"PeriodicalIF":6.7,"publicationDate":"2026-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13552327/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148912525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of chlorogenic acid disinfection on the oral microbiota of alginate impressions.","authors":"Tiehan Cui, Meichun Hu, Shuling Jiang, Fuqing Chen, Qiaoqiao Hu, Liping Yang, Na Li","doi":"10.1080/20002297.2026.2727218","DOIUrl":"10.1080/20002297.2026.2727218","url":null,"abstract":"<p><strong>Background: </strong>Cross-infection may occur if alginate impressions are not disinfected. Available chlorine (AC), though common, poses safety risks. Chlorogenic acid (CA), a natural compound, shows effective disinfection and, as we previously verified, inhibits pathogens without damaging impressions.</p><p><strong>Objective: </strong>This study investigates the influence of CA and AC on the oral microbial community of alginate impressions from a community-ecology perspective.</p><p><strong>Methods: </strong>The maxillary impressions of 60 participants were randomly assigned to four groups (<i>n</i> = 15). The NS-AC group received a normal saline (NS) rinse and a 2,000 mg/L AC spray. The NS-CA group got an NS rinse and a CA (10 mg/mL mix + 60 mg/mL spray). The CA-AC group obtained a 10 mg/mL CA rinse and a 2,000 mg/L AC spray. The CA-CA group acquired a CA (10 mg/mL rinse & mix + 60 mg/mL spray). An analysis of 16S rRNA sequencing was conducted on the samples that were collected.</p><p><strong>Results: </strong>The NS-CA group exhibited substantially higher Chao1, Shannon and Simpson indices than the NS-AC group (<i>p</i> < 0.05), and the PCoA revealed a centralized clustering pattern. As the primary biomarkers in the NS-CA, <i>Neisseria</i>, <i>Porphyromonas</i> and <i>Prevotella</i> were identified through LEfSe analysis and a random forest model (LDA > 4, AUC = 0.911). A synchronous decrease in the NS-CA was predicted by FAPROTAX functional prediction in relation to <i>aromatic_compound_degradation</i> and <i>human_pathogens_all</i>. The NS-CA and CA-CA did not exhibit any significant differences in alpha diversity or FAPROTAX (<i>p</i> > 0.05), and the PCoA showed that the two groups were closely clustered.</p><p><strong>Conclusion: </strong>The stabilization and maturation of oral microbiota on the surface of alginate impressions are facilitated by CA disinfection. Of all the groups, the NS-CA group demonstrates specific cost-effectiveness advantages and has the potential to serve as an alternative to conventional AC disinfection.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2727218"},"PeriodicalIF":6.7,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13543842/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148897539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}