Journal of Oral Microbiology最新文献

{"title":"The role of extracellular vesicles in intercellular communication within the oral cavity.","authors":"Abdelhabib Semlali, Mohammed Al-Zharani, Jassem Al-Ansari","doi":"10.1080/20002297.2026.2673760","DOIUrl":"10.1080/20002297.2026.2673760","url":null,"abstract":"<p><strong>Background: </strong>Extracellular vesicles (EVs), including exosomes, microvesicles, and apoptotic bodies, are nanoscale membrane-bound structures that mediate intercellular communication through the transfer of proteins, lipids, and nucleic acids. In the oral cavity, EVs are present in saliva and gingival crevicular fluid, where they contribute to immune regulation, epithelial barrier integrity, and host-microbiome interactions.</p><p><strong>Objective: </strong>This review aimed to critically summarize current knowledge regarding EV biogenesis, molecular composition, cellular and microbial sources, and their roles in oral homeostasis and disease pathogenesis.</p><p><strong>Results: </strong>Current evidence demonstrates that EVs derived from host cells and oral microorganisms actively regulate inflammatory responses, biofilm dynamics, tissue regeneration, and microbial colonization. Dysregulation of EV-mediated communication has been implicated in the development and progression of periodontal disease, oral candidiasis, and oral squamous cell carcinoma (OSCC). In addition, salivary EVs have emerged as promising non-invasive biomarkers and potential therapeutic tools in oral medicine. However, important methodological limitations remain, particularly regarding EV isolation, characterization, and standardization.</p><p><strong>Conclusions: </strong>EVs represent central mediators of communication within the oral microenvironment and play dual roles in maintaining oral homeostasis and promoting disease progression. Further standardized and clinically relevant studies are required to better define EV biology and facilitate the translation of EV-based diagnostic and therapeutic strategies into clinical oral medicine.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2673760"},"PeriodicalIF":6.7,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13202678/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148042536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR spacer profiling and prophage mining reveal diverse bacteriophages associated with <i>Streptococcus Mutans</i>.","authors":"Xiaolin Chen, Mingrui Zhang, Liuchang Yang, Yuxing Chen, Yaqi Chi, Yiran Zhao, Zhe Ma, Yongliang Li, Xiaoyan Wang","doi":"10.1080/20002297.2026.2674332","DOIUrl":"10.1080/20002297.2026.2674332","url":null,"abstract":"<p><strong>Background: </strong>Streptococcus mutans is a key cariogenic bacterium. Current antimicrobials lack species specificity, while phage-based approaches remain experimental and require more <i>S. mutans</i> phage isolates.</p><p><strong>Objective: </strong>To profile the diversity of <i>S. mutans</i>-associated phages and strain-level heterogeneity in phage exposure using genome-informed CRISPR spacer and prophage analyses.</p><p><strong>Materials and methods: </strong>We compiled 944 publicly available <i>S. mutans</i> genomes and dereplicated them into 735 non-redundant strains. CRISPR-Cas systems, spacers, spacer targets, and putative prophages were identified, quality-assessed, and functionally annotated. Phylogenetic relationships of (pro)phages were evaluated using terminase large subunit proteins, and comparative genomics compared spacer-positive and spacer-negative strains.</p><p><strong>Results: </strong>CRISPR systems were detected in 548/735 strains, yielding 14,263 spacers, 1,864 phage-targeting spacers mapped to 110 viral genomes, including 41 cultured isolates, 51 metagenome-assembled phages, and 18 uncultured viral genomes. The most frequently targeted cultured phage was phiKSM96, whereas metagenome-assembled <i>Caudoviricetes</i> ctNo011 showed broader targeting. Prophage mining identified 186 regions in 130 strains, including 37 of ≥ medium quality and elements related to ctNo011 and phiKSM96. TerL phylogeny showed that most high-quality endogenous prophages clustered with phiKSM96 and ctNo011.</p><p><strong>Conclusion: </strong>These findings reveal a vast, uncultivated phage repertoire targeting <i>S. mutans</i>, providing a critical genomic roadmap to guide the future isolation of novel phages for caries prevention.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2674332"},"PeriodicalIF":6.7,"publicationDate":"2026-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13195714/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148016166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of digital colorimetric analysis as an objective indicator representing microbial diversity and biological succession of dental plaque.","authors":"Ji-Na Lim, Yunseok Oh, Kyudong Han, Seyoung Mun, Jae-Young Lee","doi":"10.1080/20002297.2026.2670840","DOIUrl":"10.1080/20002297.2026.2670840","url":null,"abstract":"<p><strong>Background: </strong>Visual assessment of dental plaque is limited by subjectivity and variability in imaging conditions.</p><p><strong>Objective: </strong>This study aimed to objectively quantify plaque maturation using CIE <i>L*a*b*</i>-based color difference (<i>ΔE*</i>) and evaluate its association with microbial community structure and pathogenicity.</p><p><strong>Design: </strong>Dental images were obtained from 30 participants after application of a plaque-disclosing agent. Plaque color was quantified as <i>ΔE*</i> relative to unstained tooth surfaces and classified into Low-<i>ΔE</i>* and High-<i>ΔE*</i> groups. Saliva and supragingival plaque samples from Low-<i>ΔE*</i> and High-<i>ΔE*</i> sites were collected, and full-length 16S rRNA gene sequencing was performed using the PacBio Sequel IIe platform.</p><p><strong>Results: </strong>Saliva showed significantly higher microbial diversity than plaque samples. The Low-<i>ΔE*</i> group was dominated by early colonizers, including <i>Streptococcus sanguinis</i>, whereas the High-ΔE* group showed enrichment of anaerobic and periodontal disease-associated taxa, including <i>Prevotella</i>, <i>Treponema</i>, <i>Selenomonas</i>, <i>Campylobacter</i>, and <i>Porphyromonas endodontalis</i>. The lightness value (<i>L*</i>) was negatively correlated with periodontal pathogens, including <i>Treponema denticola</i> and <i>Tannerella forsythia</i>. PICRUSt2 analysis indicated upregulation of inflammatory pathways, including NOD-like receptor signaling and apoptosis, in the High-<i>ΔE*</i> group.</p><p><strong>Conclusions: </strong><i>ΔE*</i>-based colorimetric analysis may serve as an objective indicator of dental plaque maturation and pathogenic potential, supporting personalized oral hygiene feedback.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2670840"},"PeriodicalIF":6.7,"publicationDate":"2026-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13159611/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147930402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteriome-based oral dysbiosis index in patients with oral squamous cell carcinoma.","authors":"David Szaraz, Jan Bohm, Ctirad Machacek, Gabriela Salokova, Daniela Gachova, Filip Ruzicka, Zdenek Danek, Tarik Gheit, Jiri Zavadil, Petra Borilova Linhartova","doi":"10.1080/20002297.2026.2668149","DOIUrl":"10.1080/20002297.2026.2668149","url":null,"abstract":"<p><strong>Background: </strong>Oral dysbiosis plays an important role in the pathogenesis of oral squamous cell carcinoma (OSCC). Our study aimed to perform a pairwise comparison of the oral microbiota, especially the bacteriome, from OSCC tumoral surface vs other oral samples and evaluate the association of a novel bacteriome-based Oral Dysbiosis Index (bbODI) with the OSCC surface.</p><p><strong>Materials and methods: </strong>This pilot observational study used 84 patient-matched samples from the OSCC tumoral surface (swabs and biopsies), healthy oral mucosa (tongue and buccal swabs), and supragingival dental plaque swabs. Bacteriomes were analyzed by 16S rRNA amplicon sequencing. The presence of microscopic fungi and selected viruses was also evaluated.</p><p><strong>Results: </strong>The relative abundance of the genus <i>Fusobacterium</i>, the ratio of the relative abundances of gram-negative to gram-positive bacterial genera, and the bbODI on the tumour surface significantly differed from patient-matched healthy oral mucosa (both buccal and tongue swabs) and supragingival dental plaque samples. Oral candidosis was found in 25% of patients; all patients were negative for cytomegalovirus and Epstein-Barr virus.</p><p><strong>Conclusions: </strong>Certain characteristics of the bacteriome composition of the OSCC surface differ from patient-matched samples of healthy oral mucosa and supragingival dental plaque. The proposed bbODI appears to be a promising non-invasive tool for the identification of bacteriome disruption on the OSCC surface.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2668149"},"PeriodicalIF":6.7,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13159579/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147930419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-infection with Cbp⁺ <i>Streptococcus mutans</i> and <i>Candida albicans</i> is associated with root caries in older adults.","authors":"Bruna Albuquerque Garcia, Louise Morais Dornelas-Figueira, C Katrak, Luiz Fernando Wurdig Roesch, Scott Lance Tomar, Grace Adams, Dayane Oliveira, Marcelle Matos Nascimento, Jacqueline Abranches","doi":"10.1080/20002297.2026.2667029","DOIUrl":"10.1080/20002297.2026.2667029","url":null,"abstract":"<p><strong>Background: </strong>Gingival recession increases with age, exposing root surfaces and raising susceptibility to root caries. As the global population ages, root caries prevalence is expected to rise, underscoring the need to better understand its risk factors.</p><p><strong>Objective: </strong>To investigate the association between co-infection with Cbp⁺ <i>Streptococcus mutans</i> and <i>Candida albicans</i> and root caries in older adults with gingival recession, while characterizing the root plaque microbiome and evaluating multifactorial risk factors.</p><p><strong>Design: </strong>This cross-sectional study included 117 adults ≥65 years; 56 with root caries and 61 without. Saliva and site-specific supragingival plaque samples were collected to assess Mutans streptococci and <i>Candida</i> spp., along with microbiome composition. Demographic, behavioral, biological, dental, and medical factors were also evaluated.</p><p><strong>Results: </strong>Participants with root caries had higher salivary levels of Mutans streptococci and <i>Candida</i> spp. (p = 0.0122 and p = 0.0013). Co-infection with Cbp⁺ <i>S. mutans</i> and <i>C. albicans</i> was significantly associated with root caries (p = 0.0003). Microbiome analysis showed enrichment of <i>Capnocytophaga leadbetteri</i> in diseased root surfaces. Individuals with root caries were more likely to report xerostomia and less likely to use an electric toothbrush or floss daily.</p><p><strong>Conclusions: </strong>These findings highlight microbial and behavioral factors associated with root caries, offering insights into potential prevention and management strategies.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2667029"},"PeriodicalIF":6.7,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13151771/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147868724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial signatures of dental caries in the incarcerated elderly: a salivary microbiota study in a restricted environment.","authors":"Teeratas Kijpornyongpan, Annop Krasaesin, Thananya Chongcharoenkit, Khanti Rattanapornsompong, Avirut Truntipakorn, Patita Bhuridej, Nadnudda Rodthongkum, Sung-Dae Cho, Thantrira Porntaveetus","doi":"10.1080/20002297.2026.2662787","DOIUrl":"10.1080/20002297.2026.2662787","url":null,"abstract":"<p><strong>Background: </strong>Dental caries is driven by microbial dysbiosis and influenced by diet and lifestyle. Incarcerated populations living under regulated regimens offer a unique model to study the oral microbiota in older adults by minimising environmental confounding variables.</p><p><strong>Objectives: </strong>This study aimed to characterise the salivary microbiota of older incarcerated adults and identify bacterial taxa associated with caries status and severity.</p><p><strong>Design: </strong>Twenty-eight incarcerated men (aged ≥ 50 years) were stratified into caries-active (CA) and caries-free (CF) groups (<i>n</i> = 14 each). The salivary microbiota was profiled using 16S rRNA gene sequencing to assess diversity and differential taxonomic abundance.</p><p><strong>Results: </strong>CA subjects exhibited higher genus richness and beta-dispersion compared to CF controls. The CF group was enriched with <i>Haemophilus parainfluenzae</i>, <i>Aggregatibacter</i> sp. HMT-949 and <i>Riemerella</i> sp. HMT-322. Conversely, the CA group harboured elevated levels of <i>Dialister invisus</i>, <i>Megasphaera micronuciformis</i>, <i>Prevotella intermedia</i>, <i>Selenomonas sputigena</i>, <i>Capnocytophaga ochracea</i> and <i>Gemella haemolysans</i>. Furthermore, <i>G. haemolysans, Solobacterium moorei</i> and <i>Streptococcus</i> were positively correlated with caries severity, whereas <i>Veillonella rogosae</i> and <i>Streptococcus koreensis</i> and <i>Peptostreptococcus</i>exhibited negative correlation.</p><p><strong>Conclusion: </strong>This study elucidates salivary dysbiotic signatures in older adults within a controlled environment. The identified bacterial profiles provide biomarkers for caries risk, underscoring the need for targeted oral health surveillance and preventative strategies in institutionalised populations.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2662787"},"PeriodicalIF":6.7,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13130238/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147816241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment of periodontal biofilms via nitric oxide-augmented phototherapy.","authors":"Courtney R Johnson, Tsian D Ramrattan, Casimir J Schaefer, Rajnish Kumar, Simon J Reinhard, Shannon M Wallet, Mark H Schoenfisch","doi":"10.1080/20002297.2026.2658899","DOIUrl":"10.1080/20002297.2026.2658899","url":null,"abstract":"<p><strong>Objectives: </strong>This study investigated the antibacterial action of photo-liberated nitric oxide (NO) from primary and tertiary <i>S-</i>nitrosothiol-modified mesoporous silica nanoparticles (1° RSNO-MSNs and 3° RSNO-MSNs, respectively) against planktonic periodontal pathogens and ex vivo subgingival periodontal biofilms.</p><p><strong>Materials and methods: </strong>The antibacterial and antibiofilm efficacy of photo-liberated NO release from 1° RSNO-MSNs and 3° RSNO-MSNs was evaluated as a function of wavelength (405 and 455 nm), irradiance (400-1000 mW cm^-2), and time (0-15 min) against <i>Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis,</i> and ex vivo subgingival biofilms. Time course, biofilm dispersion, and biofilm eradication assays were employed to evaluate the synergistic impact of antibacterial blue light therapy (aBLT) and photo-liberated NO.</p><p><strong>Results: </strong>The addition of photo-initiated NO release with aBLT significantly improved antibiofilm activity, with irradiation of 405 nm at 1000 mW cm^-2 demonstrating the most efficacious NO liberation from both 1° RSNO-MSNs and 3° RSNO-MSNs. While aBLT demonstrated antibacterial activity alone, the addition of NO was crucial for biofilm dispersal and eradication.</p><p><strong>Conclusions: </strong>The in vitro and ex vivo results demonstrate, for the first time, the utility of a dual-action aBLT and NO-releasing system for treating periodontal pathogens and biofilms.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2658899"},"PeriodicalIF":6.7,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13126946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147816327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metagenome-based virome analysis identifies the oral viral signatures for periodontitis.","authors":"Tianshu Chu, Jian Liu, Yue Zhang, Kai Yang, Shenghui Li, Qiulong Yan, Yuhong Li","doi":"10.1080/20002297.2026.2662091","DOIUrl":"10.1080/20002297.2026.2662091","url":null,"abstract":"<p><strong>Background: </strong>Periodontitis (PD) is a chronic infectious disease driven by bacterial biofilms, yet the oral virome's role in pathogenesis remains poorly understood.</p><p><strong>Objective: </strong>This cross-cohort meta-analysis aims to define PD-associated viral signatures, characterize predicted virus-host interactions, and evaluate the diagnostic potential of viral biomarkers.</p><p><strong>Methods: </strong>We integrated 89 saliva (44 PD, 45 healthy) and 86 subgingival plaque (48 PD, 38 healthy) metagenomes from six public cohorts for a unified virome analysis.</p><p><strong>Results: </strong>We identified 156 viral operational taxonomic units (vOTUs) significantly associated with PD (105 in saliva, 66 in subgingival plaque and 15 shared). PD-enriched vOTUs were predicted to target periodontal pathogens including <i>Porphyromonas gingivalis</i>, whereas <i>Streptococcus</i>-targeting phages were decreased. PD-associated vOTUs harbored diverse bacterial defense and anti-defense systems, with those enriched in PD overrepresenting lysozyme and replication-associated genes. Diagnostic models based on key viral markers achieved robust performance, with AUCs of 0.95 (saliva) and 0.92 (subgingival plaque) for classifying PD.</p><p><strong>Conclusion: </strong>This study delineates a distinct oral virome profile in PD, highlights predicted virus-host interactions, and underscores the potential of viral biomarkers for PD diagnosis,providing a basis for future investigations into viral ecology and phage-based interventions.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2662091"},"PeriodicalIF":6.7,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13112875/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147774084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent perspectives on precision-targeting therapy against oral biofilm.","authors":"Lixuan Wu, Yuwang Tu, Suli Xiao, Jianchai Zeng, Guanwen Sun, Yijun Li","doi":"10.1080/20002297.2026.2662093","DOIUrl":"10.1080/20002297.2026.2662093","url":null,"abstract":"<p><strong>Background: </strong>Oral biofilm-derived diseases pose a significant clinical challenge due to their persistent nature and increasing drug resistance, contributing to a substantial global economic burden. Conventional treatments-such as mechanical debridement, antiseptic agents, and laser therapy-though partially effective, often lack specificity, resulting in non-targeted microbial killing and disruption of the ecological balance.</p><p><strong>Objective: </strong>This review provides an updated overview of the application of precision antimicrobial therapies against oral biofilms, with a particular focus on pH-responsive materials and bacteriophage-based strategies.</p><p><strong>Design: </strong>A comprehensive literature search was conducted across PubMed and Google Scholar databases from January 2016 to January 2026. A total of 84 full-text articles were included for qualitative synthesis.</p><p><strong>Results: </strong>The collective findings demonstrate that multiple precision-targeting strategies-spanning from bacteriophage therapy to pH-responsive antimicrobial materials-exhibit distinct advantages in combating oral biofilms.</p><p><strong>Conclusion: </strong>The common core principle underpinning these approaches lies in their 'precision-targeting' capability: the ability to identify and interfere with specific targets or biological processes. This attribute not only significantly enhances therapeutic efficacy but also paves the way for developing personalized, microbiome-preserving strategies for the prevention and management of oral diseases.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2662093"},"PeriodicalIF":6.7,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13103991/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147774059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating oral microbiome profiles of children with cleft lip and palate in different states of caries.","authors":"Zhelin Shi, Yuehua Zhang, Yang Chen, Siying Wu, Zhuojun Zhou, Zhenqi Chen","doi":"10.1080/20002297.2026.2660486","DOIUrl":"10.1080/20002297.2026.2660486","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the characteristics of oral salivary microbiota in children with cleft lip and palate (CLP) under different states of caries.</p><p><strong>Methods: </strong>Based on the presence or absence of CLP and the total number of decayed, missing and filled surfaces, 105 participants were divided into four groups: children with CLP and high caries (CLP-HC), children with CLP and low caries (CLP-LC), children without CLP but with high caries (NCLP-HC) and children without CLP but with low caries (NCLP-LC). Saliva samples from them were analyzed using 16S rRNA gene sequencing.</p><p><strong>Results: </strong>Alpha diversity differed significantly among the four groups with Ace and Chao indices, but not with Shannon and Simpson. Principal coordinate analysis based on the Weighted-UniFrac distance matrix showed no significant differences among the four groups, while results from the Unweighted-UniFrac distance matrix revealed significant differences. CLP-HC and CLP-LC had significantly higher Microbial Dysbiosis Index than NCLP-LC. LEfSe analysis was performed for pairwise comparisons, identifying differentially enriched bacterial genera among groups. Network analysis based on Spearman correlation revealed the interaction characteristics of microbial communities in different groups.</p><p><strong>Conclusions: </strong>Differences in microbial features were observed among the four groups, which could provide new insights for personalized oral health management for CLP patients.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2660486"},"PeriodicalIF":6.7,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13097181/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147774130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}