Journal of Oral Microbiology最新文献

{"title":"CRISPR <i>cas7</i> influences the host-pathogen interaction of <i>Porphyromonas gingivalis</i>.","authors":"Nicole de Mello Fiallos, Muhammad Irfan, Jose Solbiati, Alejandro R Walker, Jorge Frias-Lopez, Frank C Gibson","doi":"10.1080/20002297.2025.2561790","DOIUrl":"https://doi.org/10.1080/20002297.2025.2561790","url":null,"abstract":"<p><strong>Introduction: </strong><i>Porphyromonas gingivalis</i>, a Gram-negative anaerobe, is a key contributor to periodontal disease. Emerging evidence suggests a role for the <i>P. gingivalis</i> CRISPR-Cas system in disease progression, although the specific roles of its components remain unclear.</p><p><strong>Objectives: </strong>Here we investigate the role of <i>cas7</i>, a Class 1 type I-B CRISPR-Cas system component, in <i>P. gingivalis</i> physiology and host interaction.</p><p><strong>Methods: </strong>We compared <i>P. gingivalis</i> wild-type and ∆<i>cas7</i> strains for growth, biofilm formation, oxidative stress resistance, and hemagglutination. Host interactions were assessed using THP-1 macrophage-like cells to evaluate intracellular survival and cytokine response. Dual RNA-seq enabled host and microbe transcriptomic profiling during cellular infection, and <i>Galleria mellonella</i> was used to assess virulence.</p><p><strong>Results: </strong>The ∆<i>cas7</i> mutant showed similar planktonic growth and biofilm formation compared to wild-type but was more sensitive to oxidative stress and had reduced hemagglutination. Although intracellular survival was unaffected, ∆<i>cas7</i> altered the host cytokine production profile. Transcriptomic analysis revealed differential gene expression linked to oxidative stress and disease progression. In vivo, ∆<i>cas7</i> infection led to a trend of increased larval mortality.</p><p><strong>Conclusion: </strong>These findings reveal a previously unrecognized role for <i>cas7</i> in modulating <i>P. gingivalis</i> virulence, offering new insights into CRISPR-Cas system functions in bacterial pathogenesis.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2561790"},"PeriodicalIF":5.5,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12486459/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145212991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is <i>Candida albicans</i> a promoting factor, rather than an inducing factor for oral cancer?","authors":"Xu Wang, Xinming Zhang, Shuangshuang Wu, Wenqing Zhang, Zhimin Yan","doi":"10.1080/20002297.2025.2564694","DOIUrl":"https://doi.org/10.1080/20002297.2025.2564694","url":null,"abstract":"<p><strong>Background: </strong><i>Candida albicans</i> (<i>C. albicans</i>) is closely associated with cancer. Whether <i>C. albicans</i>can directly induce the occurrence of oral cancer remains undetermined.</p><p><strong>Objective: </strong>This study aimed to explore the carcinogenic potential of <i>C. albicans</i> in oral cancer.</p><p><strong>Methods: </strong>Transcriptome sequencing (mRNAseq) and whole exome sequencing (WES) were performed to reveal the effects of long-term <i>C. albicans</i> stimulation on oral mucosa in mouse models. 4-nitroquinoline-1-oxide (4NQO), a mutagenic substance that mimics tobacco, was used to examine the combined role of tobacco smoking and <i>C. albicans</i> in oral carcinogenesis. Additionally, the somatic mutation landscape of chronic hyperplastic candidiasis (CHC)-a variant of oral candidiasis typically presenting with dysplasia-was characterized in biopsy tissues.</p><p><strong>Results: </strong>Long-term <i>C. albicans</i> stimulation didn't directly induce oral cancer in mouse models but significantly upregulated the expression of genes related to cell proliferation and cancer development in the oral epithelium (e.g. <i>Mki67</i>, <i>Kif11</i>, <i>Ccna2</i>, <i>Ckap2</i>, <i>Fos</i>, <i>Ccnb1</i>; <i>P </i>< 0.05). Analysis of somatic mutations in CHC revealed that <i>Candida</i> and smoking might co-contribute to this type of precancerous lesion. Furthermore, <i>C. albicans</i> stimulation activated signaling pathways involved in inflammation and immune response, and promoted cancer formation in mice pre-treated with 4NQO.</p><p><strong>Conclusion: </strong><i>C. albicans</i> alone rarely induces carcinogenesis directly but can accelerate the malignant transformation of oral mucosa when combined with smoking.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2564694"},"PeriodicalIF":5.5,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12481529/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145206735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of bovine lactoferrin supplementation and reduced iron in formula on infant oral microbiome: a randomized controlled trial.","authors":"Cynthia Anticona, Anders Esberg, Staffan K Berglund, Maria Björmsjö, Olle Hernell, Bo Lönnerdal, Ingegerd Johansson, Pernilla Lif Holgerson","doi":"10.1080/20002297.2025.2561212","DOIUrl":"10.1080/20002297.2025.2561212","url":null,"abstract":"<p><strong>Introduction: </strong>Infant formulas with reduced iron levels and lactoferrin (Lf) supplementation might mimic the beneficial effects of breast milk on the oral microbiome. This study aimed to investigate the impact of a bovine Lf-supplemented and iron-reduced formula on the oral microbiota in infants at 4, 6 and 12 months.</p><p><strong>Methods: </strong>In a double-blind controlled trial, 6-week-old formula-fed infants were randomized to receive either a formula with reduced iron levels (2 mg/L) and Lf supplementation (1 g/L) (<i>n</i> = 72), the same formula without Lf (<i>n</i> = 72), or a standard formula (8 mg iron/L) (<i>n</i> = 36). A breast-fed reference group (<i>n</i> = 72) was also included. The oral microbiota was analyzed at 4 (<i>n</i> = 244), 6 (<i>n</i> = 216) and 12 (<i>n</i> = 229) months of age using the Oxford Nanopore Technology of the 16S rRNA gene annotation (<i>e</i>HOMD database).</p><p><strong>Results: </strong>Neither the within- or between-group diversities nor overall microbiota pattern assessment revealed any statistically significant differences in microbiota composition between the formula groups. However, single species were significantly associated with specific formula-fed groups. At 6 months, breast-fed infants exhibited significantly lower species richness and distinct microbiota composition compared to the formula-fed groups.</p><p><strong>Conclusions: </strong>The effects of reduced iron levels and lactoferrin supplementation of infant formula on the oral microbiome were inconclusive.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2561212"},"PeriodicalIF":5.5,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466180/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<b>Functional characteristics of membrane vesicles produced by</b> <i><b>Streptococcus mitis</b></i>.","authors":"Airi Matsumoto, Yuichi Oogai, Haruka Kurashige, Tomoko Sumitomo, Atsushi Tabata, Masanobu Nakata","doi":"10.1080/20002297.2025.2557962","DOIUrl":"10.1080/20002297.2025.2557962","url":null,"abstract":"<p><strong>Objective: </strong>Mitis group streptococci (MGS) are the predominant oral bacteria that cause bacteremia and infective endocarditis. Although membrane vesicle (MV) secretion has been reported in <i>Streptococcus pneumoniae</i> among MGSs, comprehensive studies using various streptococcal species are limited. We aimed to determine whether MGS species produce MVs and to examine their biological functions.</p><p><strong>Materials and methods: </strong>MVs were isolated from MGS cultures using density gradient ultracentrifugation. The particle sizes of MVs were measured, and proteins in MVs were identified by liquid-chromatography tandem mass spectrometry analysis. Effects of MVs on host cells and oral pathogenic bacteria were investigated.</p><p><strong>Results: </strong>MV production was confirmed in <i>Streptococcus mitis</i> strains NCTC12261, Nm-65, and Nm-76, with particle diameters ranging from 100 to 120 nm. The MVs contained numerous cytoplasmic proteins. The MVs showed internalization into alveolar epithelial cells and induced the production of multiple cytokines, including TNF-<i>α</i>, IL-8, IL-6, IL-1β, and IL-10, in macrophages while suppressing phagocytic activity. In neutrophil-differentiated cells, MVs induced IL-8 but not TNF-<i>α</i> production. MVs from <i>S. pneumoniae</i> TIGR4 and <i>S. mitis</i> Nm-65 inhibited biofilm formation of <i>Aggregatibacter actinomycetemcomitans</i>.</p><p><strong>Conclusions: </strong>MVs play crucial roles in MGS survival strategies through immune modulation and interspecies competition, contributing to their pathogenicity and host-pathogen interactions.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2557962"},"PeriodicalIF":5.5,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12459155/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The metagenome and metabolome signatures of dental biofilms associated with severe dental fluorosis.","authors":"Penpitcha Ajrithirong, Annop Krasaesin, Wannakorn Sriarj, Patcharaporn Gavila, Wanna Chetruengchai, Kanokwan Sriwattanapong, Chawan Manaspon, Lakshman Samaranayake, Thantrira Porntaveetus","doi":"10.1080/20002297.2025.2560591","DOIUrl":"10.1080/20002297.2025.2560591","url":null,"abstract":"<p><strong>Objective: </strong>To explore the plaque biofilm microbiome associated with severe dental fluorosis (SF), and to describe its metagenome and metabolome.</p><p><strong>Methods: </strong>Sixteen plaque biofilm samples were collected from eight 6- to 15-year-old Thai children with SF and eight age-matched, caries-free and controls. Biofilms were analyzed using shotgun metagenomic sequencing, followed by bioinformatics evaluation.</p><p><strong>Results: </strong>Taxonomic profiling of biofilms from SF and controls identified a total of 12 phyla and 354 species. While alpha diversity was similar between the groups, beta diversity analysis (<i>P</i> = 0.0010) indicated distinct microbial community structures. LEfSe highlighted key discriminatory taxa: five health-associated species (<i>Actinomyces dentalis, Tannerella sp. HOT 286, Candidatus Nanosynbacter sp, Selenomonas noxia</i> and <i>Treponema sp OMZ 804</i> ) were enriched in controls, while <i>Neisseria sicca</i>, known for fluoride-sensitive esterase production, was significantly elevated in SF. Functionally, eight metabolic pathways were altered; three of these (phosphatidylcholine acyl editing, anhydromuropeptides recycling II, ubiquinol-7 biosynthesis), hypothesized to support <i>N. sicca</i> activity, were upregulated in the SF group.</p><p><strong>Conclusion: </strong>SF is associated with a significant shift in the biofilm microbiota, characterized by enrichment of <i>N. sicca</i> and a reduction in health-associated taxa. Altered metabolic pathways supporting <i>N. sicca</i> provide mechanistic insights into its role as a candidate biomarker for fluorosis, warranting further investigation.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2560591"},"PeriodicalIF":5.5,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12459186/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiome differences between trauma- and caries-derived periapical lesions using next-generation sequencing.","authors":"Jiyuan Zhan, Yinxue Huang, Xinhui Meng, Yiquan Wang, Jia Liang, Fengjiao Zhu, Rui She, Shanshan Huang, Lijun Huo","doi":"10.1080/20002297.2025.2560016","DOIUrl":"10.1080/20002297.2025.2560016","url":null,"abstract":"<p><strong>Background: </strong>While the microbiome of caries-derived periapical lesions has been extensively characterized, the microbial profile of trauma-derived periapical lesions remains poorly understood. This study aimed to characterize the apical microbiome of trauma-derived periapical lesions and identify taxonomic differences between trauma- and caries-derived periapical lesions.</p><p><strong>Methods: </strong>Twenty patients with periapical lesions were enrolled, comprising 10 trauma-derived cases (trauma group) and 10 caries-derived cases (caries group). Microbial samples were collected using sterile paper points inserted into the root canal exudate, followed by DNA extraction and Illumina sequencing of the hypervariable V3-V4 regions of the 16S rRNA gene. Bioinformatic analyses included <i>α</i>-diversity, <i>β</i>-diversity based on Bray-Curtis distance and differential abundance testing (LEfSe method with LDA score ≥ 2.0).</p><p><strong>Results: </strong>Sequencing revealed 36 bacterial phyla and 587 genera across all samples. Trauma group showed significantly greater relative abundance of <i>Campylobacter</i> (<i>P</i> = 0.002) compared to caries group, whereas <i>Prevotella</i> (<i>P</i> = 0.008), <i>Vibrio</i> (<i>P</i> = 0.041) and <i>Filifactor</i> (<i>P</i> = 0.006) exhibited reduced abundance. The core microbiota in the trauma group included <i>Phocaeicola</i>, <i>Porphyromonas</i> and <i>Pyramidobacter</i>, based on relative abundance. LEfSe analysis identified <i>Campylobacter</i> as a biomarker for the trauma group.</p><p><strong>Conclusions: </strong>Trauma-derived periapical lesions exhibited reduced microbial diversity compared to caries-derived periapical lesions, with <i>Campylobacter</i> identified as a potential pathognomonic taxon for trauma-derived periapical lesions.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2560016"},"PeriodicalIF":5.5,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12459192/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluconazole tolerance and virulence adaptations of <i><b>Candida albicans</b></i> isolated from head and neck cancer patients.","authors":"Phimchat Suwannaphong, Patcharin Thammasit, Artid Amsri, Waranyu Ueangphairot, Pooriwat Muangwong, Kittikun Kittidachanan, Imjai Chitapanarux, Jiraporn Kantapan, Nathupakorn Dechsupa, Sirida Youngchim","doi":"10.1080/20002297.2025.2559024","DOIUrl":"10.1080/20002297.2025.2559024","url":null,"abstract":"<p><strong>Background: </strong><i>C</i> <i>andida albicans</i> is the predominant opportunistic pathogen causing oral candidiasis in immunocompromised head and neck cancer (HNC) patients. Fluconazole (FLC) is commonly used for treatment and prophylaxis; however, persistent infections remain a clinical challenge during cancer therapy. We hypothesized that <i>C. albicans</i> survival under FLC exposure may be driven by the development of tolerance or resistance, accompanied by altered virulence traits.</p><p><strong>Methods: </strong>In this study, we characterized FLC susceptibility and virulence profiles of clinical <i>C. albicans</i> isolates obtained from HNC patients.</p><p><strong>Results: </strong>Most isolates were susceptible to FLC, but two tolerant phenotypes, moderate (MT) and heavy tolerance (HT), were identified. FLC prophylaxis did not significantly affect tolerance prevalence or severity. Both tolerant isolates exhibited upregulation of key resistance genes, <i>ERG11</i>. Under FLC exposure, the MT isolate modestly increased expression of <i>ALS1</i> and <i>SAP6,</i> while downregulating other virulence genes, correlating with reduced adhesion and biofilm formation. Conversely, the HT isolate upregulated <i>ALS3</i>, <i>HWP1</i>, and <i>SAP6</i>, enhancing adhesion and sustaining biofilm integrity. Despite <i>SAP6</i> upregulation in both, host cell cytotoxicity was similar.</p><p><strong>Conclusion: </strong>These findings highlight adaptive mechanisms by which FLC-tolerant <i>C. albicans</i> retain pathogenicity under antifungal stress, posing potential challenges for clinical management in HNC patients.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2559024"},"PeriodicalIF":5.5,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12459159/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and functional validation of kynureninases from oral bacteria.","authors":"Pisit Charoenwongwatthana, Halah Ahmed, Alex Charlton, Mark D Gidley, Vsevolod Telezhkin, Jamie Coulter, Chien-Yi Chang","doi":"10.1080/20002297.2025.2561213","DOIUrl":"10.1080/20002297.2025.2561213","url":null,"abstract":"<p><strong>Background: </strong>The kynurenine (KYN) pathway produces key metabolites for immunoregulation and neuromodulation in humans, but its presence and activity in the oral microbiome are unclear. This study investigates the functionality of the key kynureninase (KynU), which catalyses kynurenine to anthranilic acid (AA), in oral bacteria.</p><p><strong>Methods: </strong>Bioinformatic analysis identified putative <i>kynU</i> genes in oral bacterial genomes, and structural similarity of the predicted proteins was evaluated using Template Modeling (TM)-score and Root Mean Square Deviation (RMSD) analyses. Selected <i>kynU</i> sequences were cloned into the pBAD-His A expression vector. Enzymatic activity was accessed by quantifying AA concentrations using liquid chromatography-mass spectrometry (LC-MS).</p><p><strong>Results: </strong>Among 71 species, seven oral bacteria were identified to possess the <i>kynU</i>. Structural analyses indicated KynU from four species may fold into functional enzymes. Three recombinant KynU from <i>Burkholderia</i> <i>cepacia</i>, <i>Ralstonia</i> <i>pickettii</i>, and <i>Stenotrophomonas</i> <i>maltophilia</i> produced detectable levels of AA (21.27 ± 12.0 µM, 19.59 ± 8.6 µM, and 46.43 ± 36.8 µM, respectively), confirming functional KYN-to-AA conversion.</p><p><strong>Conclusions: </strong>This study demonstrates KynU activity in oral bacteria, revealing an unrecognised aspect of microbial metabolism with potential implications for host-microbe interactions. Further investigation is required to elucidate the biological significance of bacterial KYN metabolites and their role in oral diseases.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2561213"},"PeriodicalIF":5.5,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12451956/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145131038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral microbial signatures underlying recurrence via PI3K/AKT/mTOR pathway modulation in oral squamous cell carcinoma.","authors":"Da-Woon Kwack, Zeba Praveen, Yeon-Hee Kim, Chong Woo Yoo, Jae Hee Ko, Suk Min Youn, Joo Yong Park, Jong-Ho Lee, Sung Weon Choi, Mi Kyung Kim","doi":"10.1080/20002297.2025.2560020","DOIUrl":"10.1080/20002297.2025.2560020","url":null,"abstract":"<p><strong>Background: </strong>Oral squamous cell carcinoma (OSCC) often recurs locally, reducing survival. The oral microbiome may influence tumor recurrence, but its prognostic role is unclear. This study investigated oral microbiomes associated with OSCC recurrence and their prognostic merit.</p><p><strong>Materials and methods: </strong>Saliva samples were collected from 133 patients with OSCC. 16S rRNA gene sequencing was performed, and microbial signatures were predicted via XGBoost. Functional metagenomic prediction was conducted using PICRUSt2.</p><p><strong>Results: </strong>XGBoost identified <i>Eubacterium</i>, <i>Lactobacillus</i>, <i>Kingella</i>, <i>Paludibacter</i>, <i>Parvimonas</i>, <i>Staphylococcus</i>, and <i>Veillonella</i> as predictive for OSCC recurrence. <i>Eubacterium</i> and <i>Lactobacillus</i> were significantly enriched in recurrent disease and associated with poor survival. <i>Staphylococcus</i> and <i>Veillonella</i> were abundant in non-recurrent disease, correlating with a favorable prognosis. The microbiome-based model achieved superior predictive performance (AUC = 0.741) compared with the clinical <i>N</i>-stage model (AUC = 0.66). <i>Eubacterium</i> and <i>Lactobacillus</i> showed positive correlations with key genes, such as protein kinase B (AKT), fibroblast growth factor receptor 1 and guanine nucleotide-binding protein G subunit beta-2, within the phosphoinositide 3-kinase (PI3K)/AKT/mammalian target of rapamycin (mTOR) pathway. In contrast, <i>Veillonell</i>a was negatively correlated with these genes.</p><p><strong>Conclusions: </strong>Oral saliva microbiome profiling reveals distinct microbial patterns associated with OSCC recurrence. Our correlation-based functional predictions indicated that the enrichment of <i>Eubacterium</i> and <i>Lactobacillus</i> along with a lower abundance of <i>Veillonella</i> may influence recurrence through oncogenic PI3K/AKT/mTOR, underscoring the prognostic potential of saliva-based microbial biomarkers.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2560020"},"PeriodicalIF":5.5,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12451969/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145130986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypochlorous acid solution serves as a potential anti-biofilm therapy for periodontitis <i>via</i> targeting quorum sensing of periodontal pathogens.","authors":"Xuerong Lv, Xiang Han, Yiyang Yang, Yuzhuo Ma, Yue Wang, Kewei Zhang, Feiyang Wang, Chen Yang, Ke Yan, Xiaoqian Wang","doi":"10.1080/20002297.2025.2557959","DOIUrl":"10.1080/20002297.2025.2557959","url":null,"abstract":"<p><strong>Backgroud: </strong>Hypochlorous acid solution (HAS), a novel bio-friendly antimicrobial, has garnered attention for its antimicrobial activity, while less is known about its antibiofilm effects on periodontal pathogenic biofilms and the underlying mechanisms.</p><p><strong>Objective: </strong>This study aimed to explore HAS's antibiofilm effect on periodontal pathogenic biofilms and the potential mechanisms.</p><p><strong>Design: </strong><i>In vitro</i>, the minimum inhibitory concentration (MIC) of HAS was determined by microdilution method. Alterations in biofilms were analysed using crystal violet (CV) staining, MTT assay and microscopic imaging techniques. The biocompatibility of HAS was assessed <i>via</i> CCK-8 and scratch assays. The regulatory mechanism of HAS within biofilms were investigated using bioluminescence assays, reactive oxygen species (ROS) detection and RT‒qPCR. <i>In vivo</i>, rat periodontitis models were established. Imaging and histological techniques were employed to evaluate the inhibitory effects of HAS on alveolar bone resorption and inflammatory cytokines.</p><p><strong>Results: </strong>Compared to 0.25% NaClO solution, it exhibited better biocompatibility. HAS downregulated biofilmvirulence factors and upregulated oxidative stress response-related genes, suggesting that inducing ROS production is a crucial mechanism of HAS in biofilm inhibition. Furthermore, HAS significantly inhibited autoinducer-2 (AI-2) activity and downregulated the QS-related genes. <i>In vivo</i>, HAS significantly reduced bone resorption and periodontal inflammation.</p><p><strong>Conclusions: </strong>Given HAS's accessibility, excellent biocompatibility, and outstanding antibiofilm properties, it may offer a safe antibiofilm approach for clinical periodontal therapy, effectively removing biofilms in areas inaccessible to instrumental therapy and persistent biofilms.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2557959"},"PeriodicalIF":5.5,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12434847/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145075392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}