Journal of Oral Microbiology最新文献

{"title":"Oral microbiome as a diagnostic biomarker for pancreatic cancer: a systematic review and meta-analysis of diagnostic accuracy.","authors":"Maryam Koopaie, Mahnaz Fatahzadeh, Sajad Kolahdooz","doi":"10.1080/20002297.2025.2571188","DOIUrl":"https://doi.org/10.1080/20002297.2025.2571188","url":null,"abstract":"<p><strong>Background: </strong>This systematic review and meta-analysis aim to assess the diagnostic accuracy of the oral microbiome in detecting pancreatic cancer.</p><p><strong>Methods: </strong>A comprehensive search of relevant studies was conducted using key terms across multiple databases. The methodological quality of the included studies was assessed using the Quality Assessment Tool for Diagnostic Accuracy Studies-2 (QUADAS-2). Diagnostic accuracy metrics were calculated including specificity, sensitivity, likelihood ratios, and diagnostic odds ratio (DOR). Subgroup analyses were performed to explore the effects of oral sample collection methods, bacterial taxonomy, and oral microbiome classifications.</p><p><strong>Results: </strong>This systematic review included nine studies, comprising 188 study units with 6601 subjects The pooled specificity, sensitivity, and diagnostic odds ratio (DOR) for the use of a single oral microbiome were 0.70 (95% CI: 0.68-0.71), 0.65 (95% CI: 0.64-0.67), and 4.85 (95% CI: 4.11-5.74), respectively. Subgroup meta-analysis revealed that using multiple oral microbiome approaches could increase the DOR to 16.33. Subgroup analysis was performed based on bacterial phylum classification, multiple oral microbiomes, sampling methods, bacterial taxonomy, and subgenus-level taxonomy (<i>g_Streptococcus</i> and <i>g_Prevotella</i>).</p><p><strong>Conclusions: </strong>Oral microbiome holds promise as a diagnostic biomarker for pancreatic cancer, supporting its potential as a noninvasive tool for the screening and early detection of this malignancy.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2571188"},"PeriodicalIF":5.5,"publicationDate":"2025-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12541924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145355073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The molecular mechanism of traditional Chinese medicine in the treatment of oropharyngeal candidiasis (OPC).","authors":"Shiqi Wang, Hongchen Wang, Wenfan Wei, Tianming Wang","doi":"10.1080/20002297.2025.2572338","DOIUrl":"https://doi.org/10.1080/20002297.2025.2572338","url":null,"abstract":"<p><strong>Background: </strong>Oropharyngeal candidiasis (OPC) is a prevalent fungal infection primarily caused by <i>Candida</i> species, notably <i>Candida albicans</i>, posing significant health challenges. Traditional Chinese medicine (TCM) has gained attention due to its multifaceted pharmacological properties and lower incidence of adverse effects compared to conventional treatments.</p><p><strong>Objective: </strong>This article aims to explore the application of TCM in OPC therapy and elucidate the potential molecular mechanisms underlying its efficacy.</p><p><strong>Design: </strong>A comprehensive analysis of relevant studies and research findings was conducted to investigate the bioactive components of TCM and their mechanisms of action.</p><p><strong>Results: </strong>The analysis reveals that TCM exerts beneficial effects through inhibition of fungal growth, regulation of immune responses, and enhancement of oral microecological balance.</p><p><strong>Conclusions: </strong>TCM offers a holistic approach to managing OPC, leveraging its multifaceted mechanisms to address this common yet troublesome infection effectively.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2572338"},"PeriodicalIF":5.5,"publicationDate":"2025-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12541921/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145355089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dysbiosis of the oropharyngeal microbiota in COVID-19: distinct profiles in patients with severe respiratory symptoms.","authors":"Sunitha Kumari V S, Varsha Potdar, Manohar Shinde, Deepti Parashar, Kalichamy Alagarasu, Sarah Cherian, Mallika Lavania","doi":"10.1080/20002297.2025.2569523","DOIUrl":"10.1080/20002297.2025.2569523","url":null,"abstract":"<p><strong>Background: </strong>COVID-19 has been strongly associated with alterations in the oropharyngeal microbiota, yet the microbial features linked to disease severity remain unclear.</p><p><strong>Objective: </strong>This study aimed to elucidate the microbial signatures associated with COVID-19 disease severity.</p><p><strong>Design: </strong>16S rRNA gene sequencing was employed to profile the oropharyngeal microbiota of patients with varying degrees of COVID-19 severity.</p><p><strong>Results: </strong>A significant reduction in alpha diversity suggests a major microbial dysbiosis in critically ill patients compared to less severe cases and healthy individuals, whereas beta diversity analysis revealed a broadly conserved community structure across different groups. Comparative analysis showed significant depletion of the phylum Fusobacteriota and enrichment of bacterial families, including Corynebacteriaceae, Methylobacteriaceae, Acetobacteraceae, Bradyrhizobiaceae, Lactobacillaceae, Staphylococcaceae, Propionibacteriaceae, and Moraxellaceae. Rothia mucilaginosa was notably enriched in patients with severe respiratory symptoms, and many of the enriched taxa are known opportunistic pathogens associated with respiratory infections.</p><p><strong>Conclusion: </strong>The marked dysbiosis and enrichment of opportunistic pathogens in the oropharyngeal microbiota of critically ill patients indicate their possible role in respiratory complications. The identified microbial patterns highlight the potential of microbiome profiling as a tool for disease prognosis and guide further research into the role of microbes in COVID-19 pathogenesis and implications for treatment protocols.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2569523"},"PeriodicalIF":5.5,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12532353/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145329249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meta-omics reveals subgingival plaque reconstruction dynamics.","authors":"Fangjie Zhou, Yajie Wu, Biao Ren, Yuchuan Liu, Kaihua Luo, Qinyang Li, Fangting Huang, Xian Peng, Yuqing Li, Zhifei Su, Jiyao Li","doi":"10.1080/20002297.2025.2569528","DOIUrl":"10.1080/20002297.2025.2569528","url":null,"abstract":"<p><strong>Background: </strong>The homeostasis of the subgingival microbiome is crucial for periodontal health, although the dynamics governing its community variation remain insufficiently studied. This study aims to investigate the dynamics of subgingival microbiota reassembly after disruption, focusing on core taxa, functions, and driving forces.</p><p><strong>Methods: </strong>339 subgingival plaques in periodontally healthy states were collected before and after ultrasonic cleaning across 12 timepoints for 1 year. All samples underwent full-length 16S rRNA sequencing; 30 were selected for metagenomic sequencing.</p><p><strong>Results: </strong>Our findings revealed that disturbed subgingival microbiota underwent short-term disruptions but subsequently reverted to baseline, maintaining stability within a year. Homogeneous selection dominated assembly, driving convergent structure under consistent pressure. Such a recovery process was accompanied by key taxa increased sequentially: <i>Pseudomonas fluorescens</i> early, <i>Haemophilus parainfluenzae</i> mid-stage, and <i>Capnocytophaga spp</i>. late. Functionally, reconstruction began with energy metabolism, expanded via biofilm formation and LPS biosynthesis mid-stage, and involved late apoptosis and complex amino acid metabolism. Microbial interactions, including positive regulation from <i>Veillonella HMT 780</i> to <i>Fusobacterium HMT 248</i>, internally drove community assembly.</p><p><strong>Conclusion: </strong>Our study clarifies species and functional dynamics during subgingival microbiota reconstruction and maps time-directed networks among stage-specific bacteria, offering a theoretical basis for targeted microbiome regulation.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2569528"},"PeriodicalIF":5.5,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12519584/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145301528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of oral microbiota in digestive system diseases: current advances and perspectives.","authors":"Yaqi Li, Yiping Xin, Wenlu Zong, Xiaoyu Li","doi":"10.1080/20002297.2025.2566403","DOIUrl":"10.1080/20002297.2025.2566403","url":null,"abstract":"<p><p>The oral microbiota is intimately linked to human health and various disease states. With the advent of the Human Microbiome Project, our comprehension of the oral microbiota has substantially improved. This microbial community is not only associated with a range of oral diseases, such as dental caries and periodontal diseases, but also with numerous digestive disorders, as demonstrated by recent clinical studies. Specific bacteria residing in the oral cavity, such as <i>Porphyromonas gingivalis</i>, <i>Fusobacterium species</i> and <i>Streptococcus species</i>, have been shown to translocate to the gastrointestinal tract, thereby establishing a potential connection between the oral and gut microbiota. The transfer and ectopic colonization of oral microbiota within the gastrointestinal tract may contribute to both the onset and exacerbation of gastrointestinal diseases. Following the principles of dysregulation characteristics, mechanism research and innovative treatment, this paper systematically reviews the association between the oral microbiota and various digestive system diseases. This paper explores how specific oral microbiota drive digestive system diseases mechanisms and evaluates treatments including probiotics, prebiotics, fecal microbiota transplantation, and targeted antimicrobial therapies. By clarifying the oral-gut microbiota-disease link, it highlights oral microbiota monitoring as a promising tool for early detection, diagnosis, and therapy.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2566403"},"PeriodicalIF":5.5,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12519590/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145301531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<b>Water-insoluble exopolysaccharide synthesized by glucosyltransferases mediates the antibacterial activity of ClyR against</b> <i><b>Streptococcus mutans</b></i>.","authors":"Qizhao Ma, Xiaowan Wang, Mai Xu, Ziyi Yang, Dian Zhang, Jiamin Chen, Tao Gong, Hang Yang, Yuqing Li","doi":"10.1080/20002297.2025.2566894","DOIUrl":"10.1080/20002297.2025.2566894","url":null,"abstract":"<p><strong>Background: </strong>Dental caries is a widespread global health issue strongly associated with <i>Streptococcus mutans</i>. Bacteriophage-derived lytic enzymes such as ClyR hold considerable promise as antibacterial potential, but the molecular mechanisms underlying their activity against <i>S. mutans</i> remain unclear.</p><p><strong>Objective: </strong>This study aimed to determine the role of water-insoluble exopolysaccharides (EPS) in mediating the antibacterial activity of ClyR against <i>S. mutans</i>.</p><p><strong>Design: </strong>We compared the antibacterial effects of ClyR on <i>S. mutans</i> UA159 and its Δ<i>gtfB</i> mutant, which is characterized by reduced synthesis of water-insoluble EPS. Biofilm architecture and susceptibility were assessed using scanning electron microscopy, confocal laser scanning microscopy, and biomass quantification. Adsorption assays were conducted to evaluate the interaction between ClyR and water-insoluble EPS.</p><p><strong>Results: </strong>The Δ<i>gtfB</i> mutant exhibited significantly higher resistance to ClyR than <i>S. mutans</i> UA159, with reduced biofilm disruption and bacterial loss after treatment. <i>In vitro</i> assays confirmed that water-insoluble EPS specifically adsorbed ClyR, with binding localized to its catalytic PlyCAC domain.</p><p><strong>Conclusions: </strong>Water-insoluble EPS synthesized by <i>S. mutans</i> glucosyltransferases plays a critical role in modulating bacterial susceptibility to ClyR. These findings reveal a novel mechanism underlying bacteriophage lysin activity and highlight EPS as a potential target for enhancing ClyR efficacy against cariogenic biofilms.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2566894"},"PeriodicalIF":5.5,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12517413/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145292580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal efficacy of caffeic acid and nano-caffeic acid particles against candidiasis: an <i><b>in vitro</b></i> study.","authors":"Maede Salehi, Iman Haghani, Majid Saeedi, Katayoun Morteza-Semnani, Reza Negarandeh, Abolfazl Hosseinnataj, Ali Jafari, Anahita Lotfizadeh, Anahita Rafiei, Tahereh Molania","doi":"10.1080/20002297.2025.2564690","DOIUrl":"10.1080/20002297.2025.2564690","url":null,"abstract":"<p><strong>Background/purpose: </strong>Candidiasis is the most common oral fungal infection. Several medications have been introduced to manage this infection. This study investigated the antifungal effect of caffeic acid and nano-caffeic acid.</p><p><strong>Materials and methods: </strong>The size and particle dispersion index (PDI) of caffeic acid-containing niosome vesicles were measured after their production. The zeta potential was measured using a Zetasizer Nano ZS, and the amount of nano-caffeic acid released from the vesicles was measured. <i>Candida</i> isolates were cultured in Malt Extract Agar medium. Nystatin, fluconazole, caffeic acid and nano-caffeic acid were studied according to the Clinical and Laboratory Standards Institute (CLSI) protocol (M27-A3/S4), a broth microdilution test was performed, and the minimum inhibitory concentration (MIC) was determined. The data were analyzed using the Mann‒Whitney and Kruskal‒Wallis tests.</p><p><strong>Results: </strong>The optimal formulation had 100 mg Tween 60, 100 mg Span 60, 200 mg cholesterol, a size of 271.83 ± 3.11 nm, a PDI of 0.21 ± 0.02, a zeta potential of 5.58 ± 0.47 mV and an encapsulation efficiency (EE%) of 42.34 ± 4.34%. The size, absolute zeta potential and EE% increased significantly with increasing cholesterol content from zero to 200  mg (<i>P </i>< 0.05). Caffeic acid, nano-caffeic acid, carrier, fluconazole and nystatin had the lowest to highest antifungal activity, respectively.</p><p><strong>Conclusion: </strong>According to the MIC₅₀ and MIC₉₀ values, nystatin, fluconazole, carrier, nano-caffeic acid and caffeic acid had the highest to lowest inhibitory efficiency against <i>Candida</i> species, respectively.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2564690"},"PeriodicalIF":5.5,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12507112/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145258357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Streptococcus cristatus reduces cariogenicity of saliva-derived microcosms under pH-dependent conditions.","authors":"Yanling Cai, Lijing Wu, Bernd W Brandt, Mark J Buijs, Xi Wei, Hongyan Liu, Dongmei Deng","doi":"10.1080/20002297.2025.2565450","DOIUrl":"10.1080/20002297.2025.2565450","url":null,"abstract":"<p><strong>Background: </strong>The study aims to investigate <i>Streptococcus cristatus</i>, an oral commensal bacterium, as a probiotic for dental caries prevention by modulating the oral microbiome.</p><p><strong>Methods: </strong>Saliva from four healthy donors was used to establish 24-h microcosm biofilms in an <i>in vitro</i> 96-well peg model. The preformed biofilms were then exposed to biofilm medium containing 0.2% sucrose (BM), with or without <i>S. cristatus</i>. They were grown for 48 h under two conditions: a constant pH-neutral regime (BM supplemented with 76 mM K₂HPO₄ and 15 mM KH₂PO₄, pH 7.0) or cariogenic pH-cycling regime (8 h pH-neutral and 16 h in BM containing 100 mM acetic acid, pH 5.5). Phosphate and acetate buffers were used to control pH. After 72 h, the biofilms were analyzed for biomass, lactic acid production, hydrogen peroxide (HP) concentrations, and microbial composition via 16S rRNA gene sequencing.</p><p><strong>Results: </strong><i>S. cristatus</i> successfully integrated into 24-h preformed microcosm biofilms derived from individual saliva. Under pH-neutral conditions, it reduced biofilm biomass and lactate production while increasing hydrogen peroxide (HP) generation in a donor-dependent manner. Conversely, under cariogenic pH-cycling conditions, these inhibitory effects on biomass and lactate production were consistent across all donors, although HP was undetectable. Microbiome analysis revealed that <i>S. cristatus</i> increased species richness and mitigated the compositional shifts caused by pH-cycling. This was achieved by inhibiting <i>Streptococcus salivarius/vestibularis</i> across all donors, while promoting <i>Streptococcus mitis</i> group and <i>Streptococcus anginosus</i> in a donor-dependent manner.</p><p><strong>Conclusions: </strong><i>S. cristatus</i> represents a promising microbiome modulator with the potential to substantially mitigate the cariogenicity of oral microcosms.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2565450"},"PeriodicalIF":5.5,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12507114/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145258295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sepsis and septic shock caused by <i>Porphyromonas gingivalis</i>: a case report.","authors":"Meifang Lin, Guang Yang, Cong Shen, Yinglun Xiao, Cha Chen, Xuan Zhang","doi":"10.1080/20002297.2025.2564692","DOIUrl":"10.1080/20002297.2025.2564692","url":null,"abstract":"<p><strong>Background: </strong><i>Porphyromonas gingivalis</i> is a predominant pathogen in periodontitis and is closely associated with the progression of chronic obstructive pulmonary disease (COPD).</p><p><strong>Objective: </strong>This case report aims to describe a case of sepsis caused by <i>P. gingivalis</i> in a patient with COPD and a history of dental pain, highlighting the diagnostic challenges and clinical implications.</p><p><strong>Design: </strong>This single case report was based on clinical data collected from medical records, with the pathogen identified from blood cultures by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). A comparative analysis was performed between the present case and previously reported cases of <i>P. gingivalis</i> bacteremia or sepsis based on a literature review. The patient was discharged after his general condition improved as a result of the potentially effective antimicrobial agents and anti-infective treatments through literature review.</p><p><strong>Results: </strong>A 73-year-old man with COPD and a prolonged history of dental pain presented with a 30-year history of recurrent cough, expectoration, and dyspnoea, with symptoms exacerbating over the past 3 d and the recent onset of high fever for 1 d. Clinical evaluation revealed sepsis with rapid progression to septic shock. Blood cultures confirmed the presence of <i>P. gingivalis</i>.</p><p><strong>Conclusions: </strong>This case highlights the need to consider anaerobes like <i>P. gingivalis</i> in septic patients with poor oral health, especially for patients with dental pain or periodontitis, and highlights the diagnostic challenges associated with slow-growing pathogens.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2564692"},"PeriodicalIF":5.5,"publicationDate":"2025-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12498353/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145244646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel biomarker identification for oral squamous cell carcinoma development in nonsmoker, nondrinker, and nonchewer patients using third-generation sequencing of oral microbiome.","authors":"Wei-Ni Lyu, Cheng-Ying Shen, Yung-Hua Lee, Shin-Kuang Chen, Eric Y Chuang, Pei-Jen Lou, Mong-Hsun Tsai","doi":"10.1080/20002297.2025.2565452","DOIUrl":"10.1080/20002297.2025.2565452","url":null,"abstract":"<p><strong>Background/objective: </strong>Oral squamous cell carcinoma (OSCC) in patients without tobacco, alcohol, or betel-quid habits is poorly understood and difficult to detect early. This study aimed to identify microbial biomarkers specific to this habit-free population using third-generation sequencing (TGS).</p><p><strong>Patients/materials and methods: </strong>Twenty-seven habit-free OSCC patients were recruited at National Taiwan University Hospital (NTUH). Paired tumor and adjacent normal tissues were collected with informed consent and NTUH Research Ethics Committee approval (IRB 201902080RINC, 201304078RIND). Full-length 16S rRNA sequencing (PacBio Sequel IIe) was processed with DADA2 and SILVA. Biomarkers were identified using sparse partial least squares discriminant analysis (sPLS-DA) and random forest with cross-validation, and validated against three public OSCC cohorts.</p><p><strong>Results: </strong>A three-species panel-<i>Eikenella corrodens</i>, <i>Slackia exigua</i>, and <i>Eggerthia catenaformis</i>-discriminated tumor from normal tissues (AUC = 0.905 training; 0.733 testing). Functional and network analyses showed tumor-enriched taxa forming pro-inflammatory clusters linked to lipid and glutamine metabolism, while commensals correlated with homeostatic pathways. Cross-cohort comparison confirmed this panel's specificity to habit-free OSCC.</p><p><strong>Conclusions: </strong>Using TGS, we revealed distinct microbial signatures in habit-free OSCC that may aid early diagnosis and underscore the role of microbiome-host interactions in carcinogenesis.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2565452"},"PeriodicalIF":5.5,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12493606/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145232982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}