Journal of Oral Microbiology最新文献

{"title":"Exploring the antimicrobial potential of the articaine derivative in oral infections.","authors":"Yongzhen Tan, Yihang Hao, Yihang Fu, Shilong Hu, Xi Yang, Yaling Tang, Bowen Ke, Xinhua Liang","doi":"10.1080/20002297.2025.2502455","DOIUrl":"https://doi.org/10.1080/20002297.2025.2502455","url":null,"abstract":"<p><strong>Objective: </strong>Postoperative infection is one of the most common complications in dental procedures. During local anesthesia in dental treatments, the risk of postoperative infections increases if the oral mucosa is infected, the anesthesia injection site is inadequately disinfected, or the injection needle and anesthetic drugs are contaminated. Thus, developing new oral local anesthetics that offer superior anesthesia, enhanced safety, and antimicrobial properties could greatly enhance their clinical value.</p><p><strong>Methods: </strong>The anesthetic effects and antibacterial properties of articaine derivatives were screened using membrane chromatography techniques, animal experiments, and cellular molecular assays. Safety assessments were conducted on the selected target compounds. Additionally, the antibacterial mechanisms of the compounds were investigated through molecular dynamics simulations and cryo-electron microscopy.</p><p><strong>Results: </strong>Through the screening of articaine derivatives, a novel local anesthetic, AT-15, was identified, which combines effective anesthetic properties with antibacterial activity. This compound exhibits strong pharmacological activity and high safety. Its antibacterial effect is believed to result from the disruption of bacterial cell membranes and the inhibition of topoisomerase, an enzyme essential for bacterial DNA synthesis.</p><p><strong>Conclusion: </strong>AT-15 discovered in this study is a promising candidate for further development in clinical settings.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2502455"},"PeriodicalIF":3.7,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12068334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Patients with periodontitis exhibit persistent dysbiosis of the gut microbiota and distinct serum metabolome.","authors":"Eiji Miyauchi, Kyoko Yamazaki, Yuuri Tsuboi, Takako Nakajima, Shigeru Ono, Kentaro Mizuno, Naoki Takahashi, Kentaro Imamura, Hidetoshi Morita, Nobuaki Miura, Shujiro Okuda, Jun Kikuchi, Nobuo Sasaki, Hiroshi Ohno, Kazuhisa Yamazaki","doi":"10.1080/20002297.2025.2499284","DOIUrl":"https://doi.org/10.1080/20002297.2025.2499284","url":null,"abstract":"<p><strong>Objectives: </strong>Animal studies suggest that periodontopathic bacteria induce gut dysbiosis and related pathology, possibly connecting periodontitis to non-oral diseases. However, the effects on the gut ecosystem in periodontitis patients are not fully understood.</p><p><strong>Methods: </strong>We conducted a comprehensive analysis of the salivary and gut microbiota using 16S rRNA sequencing in periodontitis patients before and after treatment, comparing them to healthy participants. Serum metabolites were also analyzed.</p><p><strong>Results: </strong>Periodontitis patients showed high alpha diversity in both salivary and gut microbiota with a strong correlation. Significant differences were also observed in the gut microbiota composition between patients before treatment and healthy participants, irrespective of the ectopic colonization of periodontitis-associated bacteria in the gut. Co-abundance group analysis demonstrated that the gut microbiota of healthy participants was enriched with short-chain fatty acid producers. Changes in the gut microbiota coincided with alterations in the serum metabolite profile. While periodontal therapy improved salivary microbiota, it did not significantly affect gut microbiota.</p><p><strong>Conclusions: </strong>Gut dysbiosis of periodontitis patients may impact systemic metabolite profiles. Given that periodontal therapy alone did not substantially improve the gut microbiota, adjunctive strategies targeting the gut microbiome may be effective in reducing the risk of periodontitis-associated diseases.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2499284"},"PeriodicalIF":3.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12064113/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of <i>Helicobacter pylori</i> on enamel and dentin development - an <i>in vitro</i> study in mice.","authors":"Carin Sahlberg, Eija Salmela, David P Rice, Kazuhiko Nakano, Ryota Nomura, Satu Alaluusua","doi":"10.1080/20002297.2025.2500670","DOIUrl":"https://doi.org/10.1080/20002297.2025.2500670","url":null,"abstract":"<p><strong>Objective: </strong>A heavy infection in a primary molar tooth can impair the enamel formation of the underlying permanent successor. <i>Helicobacter pylori</i> colonizes primarily the stomach, but it has also been detected in oral samples, including in the dental pulp of infected primary teeth. Here, we aim to test if <i>H. pylori</i> can disturb enamel and dentin formation.</p><p><strong>Methods: </strong>Mandibular molar explants of E18.5 mice were grown for 12 days in media containing 10% of <i>H. pylori</i> cell lysates. The presence and extent of enamel and dentin on the mesial surface of the first molar explants were evaluated from stereomicroscopic photographs and histologically.</p><p><strong>Results: </strong>The statistical analyses revealed that less enamel was formed in the test (N = 47) than in the control first molars (N = 28, <i>p</i> < 0.001). Most severe disturbances were seen in explants grown in media containing <i>H. pylori</i> cell lysates, which were made from stationary growth-phase cultures, with high optical density. Histological findings showed that dentin mineralization was also impaired.</p><p><strong>Conclusion: </strong>The results suggest that H. pylori disturbs enamel and dentin development in cultured mouse embryonic molar teeth. This provides new insight into the etiology of enamel disturbances in permanent teeth.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2500670"},"PeriodicalIF":3.7,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12064110/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation of tongue coating thickness with microinflammatory state and oral microbiome in maintenance hemodialysis patients.","authors":"Yanqin Zhu, Xueyan Zeng, Aiping Zhang, Bin Lu, Mengqi Wu, Hong Liu, Fenggui Zhu, Riyang Lin","doi":"10.1080/20002297.2025.2488054","DOIUrl":"https://doi.org/10.1080/20002297.2025.2488054","url":null,"abstract":"<p><strong>Aim: </strong>This study investigated the correlation between tongue coating thickness (TCT), micro-inflammatory state (MIS), and oral microbiome in maintenance hemodialysis (MHD) patients.</p><p><strong>Methods: </strong>Forty MHD patients (20 thin-tongue coating [BTZ], 20 thick-tongue coating [HTZ]) and 15 healthy controls (DZZ) were enrolled. Blood microinflammatory markers were analyzed in all patients. Saliva samples from 15 HTZ, 15 BTZ, and 15 DZZ underwent 16S rRNA sequencing.</p><p><strong>Results: </strong>HTZ patients exhibited higher microinflammatory marker levels than BTZ. Oral microbiome species richness in DZZ surpassed that of the MHD groups, with distinct structural differences, particularly between HTZ and DZZ. HTZ showed higher abundances of <i>Actinobacillus, Peptostreptococcus</i>, and Lachnospiraceae <i>NK4A136 group</i> than BTZ. Correlation analysis revealed a positive correlation between the levels of IL-6 and TNF-α and the abundance of <i>Fusobacterium</i>, but a negative correlation with <i>Streptococcus</i>. Additionally, the TNF-α level positively correlated with <i>Campylobacter</i>.</p><p><strong>Conclusion: </strong>Thick tongue coating in MHD patients is associated with elevated microinflammation and altered oral microbiome, suggesting a link between inflammation and microbial dysbiosis.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2488054"},"PeriodicalIF":3.7,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12054557/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144030373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA virus tropism in healthy dental pulp: an in-situ reservoir site for torque teno virus and parvovirus B19.","authors":"O I Mielonen, S Hänninen, J Willberg, T Salo, M Mauramo","doi":"10.1080/20002297.2025.2499924","DOIUrl":"https://doi.org/10.1080/20002297.2025.2499924","url":null,"abstract":"<p><strong>Background: </strong>The presence of viruses in healthy teeth has not been extensively studied, although some viral traces have been detected in both healthy and diseased dental pulps in previous studies focusing primarily on a single species. The aim of this study is to clarify the persistence of DNA viruses in dental tissues and their impact on tissue composition.</p><p><strong>Materials and methods: </strong>Here, the prevalence of persistent DNA viruses in intact third molars (<i>n</i> = 17) was assessed via quantitative PCR to detect human parvovirus B19 (B19V), torque teno virus (TTV) and nine human herpesviruses. Also, H&E-stained tissue sections of the samples were analyzed for any potential inflammatory process. RNAscope in-situ hybridization was performed for B19V, TTV and HHV7 subsequently.</p><p><strong>Results: </strong>Viral DNA of five different viruses was detected in 5 of the 17 samples (29.4%) including B19V (<i>n</i> = 2), TTV (<i>n</i> = 2), HHV7 (<i>n</i> = 2), HCMV (<i>n</i> = 1) or EBV (<i>n</i> = 1) in dental pulps with no signs of cytopathic effect, inflammatory cell accumulations or necrosis. RNAscope in-situ hybridization confirmed the presence of B19V and TTV in non-inflamed pulp tissue.</p><p><strong>Conclusions: </strong>These findings emphasized that even in the absence of a disease evaluated by histology, dental pulp can harbor DNA viruses and be an anatomical site of virus tropism, suggesting viral persistence rather than direct pathogenic activity.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2499924"},"PeriodicalIF":3.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12051552/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144004426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the salivary microbiome of adults with inflammatory bowel disease.","authors":"Vanessa DeClercq, Robyn J Wright, Johan van Limbergen, Morgan G I Langille","doi":"10.1080/20002297.2025.2499923","DOIUrl":"https://doi.org/10.1080/20002297.2025.2499923","url":null,"abstract":"<p><strong>Background: </strong>Perturbations of the gut microbiota in patients with inflammatory bowel disease (IBD) have been extensively characterised, but changes to the oral microbiome remain understudied. This study aimed to evaluate the oral microbiome of adults with IBD and of matched controls.</p><p><strong>Methods: </strong>Saliva samples and data were obtained from a Canadian population cohort (<i>n</i> = 320). The salivary microbiome was characterised using 16S rRNA gene sequencing and examined for differences between control participants and those with IBD, as well as disease subcategories (Crohn's Disease and Ulcerative Colitis).</p><p><strong>Results: </strong>Alpha diversity was significantly lower in participants with IBD than controls in unadjusted models and many remained significant after adjusting for covariates. Significant differences in some beta diversity metrics between participants with IBD and controls were found, although these did not remain significant when adjusted for covariates. Ten genera were significantly differentially abundant between cases and controls. <i>Veillonella</i> and <i>Streptococcus</i> were both increased in abundance in IBD cases <i>vs</i> controls (25% <i>vs</i> 22% and 14% <i>vs</i> 12%, respectively).</p><p><strong>Conclusion: </strong>These results showcase changes in oral microbial diversity and composition in those living with IBD and highlight the potential of using the salivary microbiome as a biomarker for screening or monitoring IBD.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2499923"},"PeriodicalIF":3.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12046613/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of oral microbiome composition in elderly Chinese patients with hypertension: a cross-sectional study.","authors":"Xin Chen, Wen-Yong Lin, Feng-Wei Zhang, Li-Qiang Guo, Han Ge, Ding-Zuo Ge, Juan-Juan Tan, Bao-Cheng Liu, Rui-Rui Wang, Lei Zhang","doi":"10.1080/20002297.2025.2489603","DOIUrl":"https://doi.org/10.1080/20002297.2025.2489603","url":null,"abstract":"<p><strong>Background: </strong>Hypertension is a prevalent metabolic disorder in the elderly, with its pathogenesis linked to gut microbiota dysbiosis. Recent studies suggested that oral microbiota may also play a role in hypertension development, yet its relationship with hypertension in the elderly remains underexplored.</p><p><strong>Objective: </strong>This cross-sectional study aimed to examine the structure of the oral microbiota and its association with hypertension in elderly patients, providing insights into hypertension prevention and treatment.</p><p><strong>Methods: </strong>A total of 206 subjects (60-89 years) were categorized into normal (CON) and hypertensive (HTN) groups, based on the Chinese Hypertension Guidelines. Saliva samples were analyzed using 16S rRNA gene sequencing.</p><p><strong>Results: </strong>Oral microbiota composition was significantly influenced by blood pressure. At the phylum level, Synergistetes and Spirochaetes were more significantly abundant in the HTN group, while at the genus level Treponema and Leptothrix was higher, Actinomyces and Capnocytophaga were lower in HTN. Random Forest analysis identified 15 key microbiota as strong discriminators of HTN (AUC 0.74). Blood pressure was negatively correlated with Actinomycetes and positively correlated with Leptothrix. PICRUST2 analysis revealed elevated chlorinated compound degradation in HTN patients.</p><p><strong>Conclusions: </strong>This study identified distinct oral microbiota in elderly hypertensive patients, highlighting the role of the oral microbiome in hypertension pathogenesis.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2489603"},"PeriodicalIF":3.7,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12016255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144002668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Candida albicans</i> impacts carbohydrate metabolism of <i>Enterococcus faecalis</i> in interkingdom biofilms.","authors":"Nicole de Mello Fiallos, Iriana J Zanin Dos Santos, Debra N Brunson, Jessica K Kajfasz, Lin Zeng, Rossana de Aguiar Cordeiro, José A Lemos, Jacqueline Abranches","doi":"10.1080/20002297.2025.2492194","DOIUrl":"https://doi.org/10.1080/20002297.2025.2492194","url":null,"abstract":"<p><strong>Objectives: </strong>This study investigated the transcriptional and physiological responses of <i>Enterococcus faecalis</i>, an opportunistic pathogen linked to endodontic infections, when cultivated in dual-species biofilms with <i>Candida albicans</i>, a yeast pathobiont found in the oral cavity.</p><p><strong>Methods: </strong>Forty-eight-hour <i>E. faecalis</i> OG1RF biofilms were developed in BHI medium as mono- or dual-species with <i>C. albicans</i> SC5314. Biofilms were assessed for biomass, colony-forming units (CFUs), and architecture using confocal microscopy. RNA sequencing was performed on an Illumina platform. Mannose-PTS activity and glycerol quantification assays were conducted to investigate changes in carbohydrate metabolism.</p><p><strong>Results: </strong>Transcriptomic analysis revealed 149 <i>E. faecalis</i> genes differentially expressed in dual-species biofilms. Genes linked to mannose-PTS and glycerol metabolism were notably upregulated. Mannose-PTS activity was significantly higher in dual-species biofilms. Mannose, as the sole carbohydrate source, increased <i>E. faecalis</i> CFUs and decreased <i>C. albicans</i> CFUs in co-culture, while glucose had no effect. As <i>C. albicans</i> is a glycerol net producer, glycerol levels were always higher when <i>C. albicans</i> was present, likely contributing to the upregulation of glycerol metabolism genes in <i>E. faecalis</i> when in co-cultures.</p><p><strong>Conclusions: </strong>The presence of <i>C. albicans</i> alters <i>E. faecalis</i> gene expression and metabolism, suggesting metabolic crosstalk that may influence their pathogenicity and role in oral infections.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2492194"},"PeriodicalIF":3.7,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12004723/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144012620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary analysis of salivary microbiota in catathrenia (nocturnal groaning) using machine learning algorithms.","authors":"Min Yu, Yujia Lu, Wanxin Zhang, Xu Gong, Zeliang Hao, Liyue Xu, Yongfei Wen, Xiaosong Dong, Fang Han, Xuemei Gao","doi":"10.1080/20002297.2025.2489613","DOIUrl":"https://doi.org/10.1080/20002297.2025.2489613","url":null,"abstract":"<p><strong>Objectives: </strong>The present study aimed to characterize the salivary microbiota in patients with catathrenia and to longitudinally validate potential biomarkers after treatment with mandibular advancement devices (MAD).</p><p><strong>Materials and methods: </strong>Twenty-two patients with catathrenia (12 M/10 F, median age 28 y) and 22 age-matched control volunteers (8 M/14 F, median age 30 y) were included in the cross-sectional study. Video/audio polysomnography was conducted for diagnosis. All patients received treatment with custom-fit MAD and were followed for one month. Ten patients (6 M/4 F) underwent post-treatment PSG. Salivary samples were collected, and microbial characteristics were analyzed using 16S rRNA gene sequencing. The 10-fold cross-validated XGBoost and nested Random Forest Classifier machine learning algorithms were utilized to identify potential biomarkers.</p><p><strong>Results: </strong>In the cross-sectional study, patients with catathrenia had lower α-diversity represented by Chao 1, Faith's phylogenetic diversity (pd), and observed species. Beta-diversity based on the Bray-Curtis dissimilarities revealed a significant inter-group separation (<i>p</i> = 0.001). The inter-group microbiota distribution was significantly different on the phylum and family levels. The treatment of MAD did not alter salivary microbiota distribution significantly. Among the most important genera in catathrenia and control classification identified by machine learning algorithms, four genera, <i>Alloprevotella, Peptostreptococcaceae_XI_G1, Actinomyces</i> and <i>Rothia</i>, changed significantly with MAD treatment. Correlation analysis revealed that <i>Alloprevotella</i> was negatively related to the severity of catathrenia (r²= -0.63, <i>p</i> < 0.001).</p><p><strong>Conclusions: </strong>High-throughput sequencing revealed that the salivary microbiota composition was significantly altered in patients with catathrenia. Some characteristic genera (<i>Alloprevotella, Peptostreptococcaceae_XI_G1, Actinomyces,</i> and <i>Rothia</i>) could be potential biomarkers sensitive to treatment. Future studies are needed to confirm and determine the mechanisms underlying these findings.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2489613"},"PeriodicalIF":3.7,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12004722/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Odontogenic brain abscess caused by <i>Porphyromonas gingivalis</i> and <i>Streptococcus constellatus</i>: a case report and review article.","authors":"Siyu Sun, Rui He, Shan Chen, Jing Ren, Xinrong Ma, Junying Yang","doi":"10.1080/20002297.2025.2485197","DOIUrl":"https://doi.org/10.1080/20002297.2025.2485197","url":null,"abstract":"<p><strong>Background: </strong>Odontogenic brain abscess is a rare, but potentially fatal, central nervous system infection, with insidious onset and unclear etiology.</p><p><strong>Methods: </strong>This case reports a 70-year-old male patient who developed an odontogenic brain abscess secondary to periodontal infection and underwent neurological surgery. Extract pus during surgery for the metagenomic next-generation sequencing (mNGS).</p><p><strong>Results: </strong>The mNGS of pus samples obtained from brain abscess aspiration identified the periodontal pathogens <i>Porphyromonas gingivalis</i> and <i>Streptococcus constellatus</i>. Consequently, he was referred to the department of stomatology for further examination and treatment.</p><p><strong>Conclusions: </strong>Our study found that major periodontal pathogens including <i>P. gingivalis</i> and <i>S. constellatus</i> were essential in the development of odontogenic brain abscesses; thus, timely intervention and preventive measures are important for treatment.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2485197"},"PeriodicalIF":3.7,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12001842/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144000152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}