Journal of Oral Microbiology最新文献

{"title":"Response to Letter to the Editor on: 'association between dental caries and self-reported COPD prevalence in U.S. adults aged ≥60 years: a cross-sectional analysis of NHANES 2015-2020'.","authors":"Wentao Lin, Chengyu Huang, Wen Zhou","doi":"10.1080/20002297.2026.2744235","DOIUrl":"10.1080/20002297.2026.2744235","url":null,"abstract":"","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2744235"},"PeriodicalIF":6.7,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13665691/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149194602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From oral dysbiosis to digital public health pathways: translating Indonesian HIV oral microbiome evidence into integrated care.","authors":"Deby Febriyan Eprilianto, Yuyun Eka Kartika Sari","doi":"10.1080/20002297.2026.2746874","DOIUrl":"10.1080/20002297.2026.2746874","url":null,"abstract":"","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2746874"},"PeriodicalIF":6.7,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13665692/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149194639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut oralisation signatures in Parkinson's disease: an integrative multi-study analysis.","authors":"Gabriela Durão, Emile Boyer, Martine Bonnaure-Mallet, Marc Verin, Manon Auffret, Vincent Meuric","doi":"10.1080/20002297.2026.2737700","DOIUrl":"https://doi.org/10.1080/20002297.2026.2737700","url":null,"abstract":"<p><strong>Background: </strong>Parkinson's disease (PD) compromises oral hygiene and oral health, potentially increasing oral bacterial input to the gut. Whether PD is associated with reproducible faecal enrichment of oral disease-linked taxa remains unclear.</p><p><strong>Objective: </strong>To determine whether oral disease-linked taxa are enriched in the faecal microbiota of individuals with PD.</p><p><strong>Methods: </strong>We conducted a pooled re-analysis of 23 faecal 16S rRNA datasets, comprising 3,594 PD and control samples. eHOMD was used to resolve oral-associated taxa, including caries/endodontic- and periodontitis-linked taxa. SILVA-based profiles were used to assess community diversity and ordination patterns. Differential-abundance analyses accounted for variation between studies.</p><p><strong>Results: </strong>PD samples showed heterogeneous but reproducible enrichment of oral disease-linked taxa, with a prominent caries/endodontic-associated component. The cariogenic-enriched subgroup showed reduced evenness and increased representation of oral taxa including <i>Streptococcus mutans</i>. Beta-diversity analyses showed substantial PD-control overlap and an oralization-related compositional gradient rather than a discrete PD-specific cluster.</p><p><strong>Conclusions: </strong>This pooled re-analysis identifies a gut oralization-associated signal in a subset of individuals with PD. Faecal enrichment of oral-associated taxa may represent one component of gut microbiome heterogeneity and may indirectly reflect variation in oral ecosystem status, supporting oral health assessment in future PD microbiome studies.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2737700"},"PeriodicalIF":6.7,"publicationDate":"2026-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13656644/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149182534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral microbiome and postmenopausal breast cancer risk: findings from the NIH-AARP diet and health study.","authors":"Giovanny Herrera, Xing Hua, Zeni Wu, Linda M Liao, Yunhu Wan, Casey L Dagnall, Kristine Jones, Belynda D Hicks, Amy Hutchinson, Mitchell H Gail, Jianxin Shi, Gretchen L Gierach, Rashmi Sinha, Emily Vogtmann","doi":"10.1080/20002297.2026.2728745","DOIUrl":"https://doi.org/10.1080/20002297.2026.2728745","url":null,"abstract":"<p><strong>Introduction: </strong>Breast cancer is the most frequently diagnosed cancer among women worldwide. Some case-control studies have reported associations between the oral microbiome and breast cancer, but the temporality of the association remains unclear. Therefore, we examined this association prospectively in the NIH-AARP Diet and Health Study.</p><p><strong>Methods: </strong>In 2005, oral wash samples were collected from a subset of the NIH-AARP cohort. We identified 318 women who developed breast cancer after collection and 318 controls, incidence-density matched on age at collection, race/ethnicity and smoking. DNA was extracted using the DNeasy PowerSoil Pro Kit, followed by shotgun metagenomic sequencing. Conditional logistic regression was used to calculate the odds ratios (OR) for associations between alpha- and beta-diversity and species- and gene-level metrics with breast cancer risk, adjusting for potential confounders.</p><p><strong>Results: </strong>Cases developed breast cancer on average 3.4 years after sample collection. Overall, the majority of the microbial metrics, including species and genes, showed largely null associations with the exception of Pielou's evenness (OR = 0.82; 95% CI = 0.70-0.95). When stratified by time to diagnosis, the inverse association with Pielou's evenness was only detected among women diagnosed within 2 years (29% of cases). In this subgroup, some previously reported associations, like the inverse association with the presence of <i>Treponema</i> (OR = 0.37; 95% CI = 0.17-0.78), were replicated. Null associations were predominantly observed for cases diagnosed more than 2 years after sample collection.</p><p><strong>Conclusions: </strong>The oral microbiome was generally not prospectively associated with postmenopausal breast cancer in a US population and results suggested previous findings may reflect reverse causation.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2728745"},"PeriodicalIF":6.7,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13656656/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149182626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Site-stratified global oral <i>Helicobacter pylori</i> prevalence: an updated meta-analysis of dental plaque and saliva studies (1989-2026).","authors":"Yutong Liu, Yanlin Zeng, Jingxiao Hu, Xiaolan Li","doi":"10.1080/20002297.2026.2739484","DOIUrl":"10.1080/20002297.2026.2739484","url":null,"abstract":"<p><strong>Background: </strong>The oral cavity is a proposed extra-gastric reservoir of <i>Helicobacter pylori</i>, but site-stratified oral prevalence has not been updated since the 2025 dental-plaque synthesis of Anand et al., and 2024-2026 evidence remains unincorporated.</p><p><strong>Materials and methods: </strong>We pooled oral <i>H. pylori</i> prevalence by site from 53 site-observations (49 unique study cohorts; 6,247 participants; 24 countries; 1989-2026): 33 plaque site-observations from Anand et al. (2025) plus 20 site-observations from our independent update, each verified at the abstract or full-text level. Random-effects (DerSimonian-Laird, logit) pooling with prediction intervals, subgroup, meta-regression and Egger analyses was used.</p><p><strong>Results: </strong>Pooled dental-plaque prevalence was 50.2% (95% CI 43.4-57.0%; I² = 92.2%; 39 studies) versus 32.4% (95% CI 21.2-45.9%; I² = 97.2%; 10 studies) in saliva; caries, oral-mixed, tongue data were too sparse to pool. Plaque prevalence was numerically higher than saliva (ratio 1.55; 95% CI 0.82-2.93). PCR (45.3%) exceeded culture (39.7%); regional prevalence ranged from 31.4% (Western Pacific) to 63.0% (South-East Asia).</p><p><strong>Conclusions: </strong>Dental plaque is the dominant oral <i>H. pylori</i> reservoir (pooled prevalence > 50%), suggesting that plaque-targeted decontamination merits evaluation as an adjunct to gastric eradication rather than being an established indication; extreme heterogeneity warrants confirmation in harmonised multi-site studies.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2739484"},"PeriodicalIF":6.7,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13647345/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149136630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction.","authors":"","doi":"10.1080/20002297.2026.2743220","DOIUrl":"https://doi.org/10.1080/20002297.2026.2743220","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1080/20002297.2026.2723644.].</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2743220"},"PeriodicalIF":6.7,"publicationDate":"2026-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13644446/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149136645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The V-ATPase V _<b>0</b> c' subunit is critical for <i>Candida albicans</i> pathogenicity in oral epithelial infection.","authors":"Zheng Wang, Shenglan Long, Han Du, Xiaomeng Liu, Yiding Li, Qian Jiang, Lang Cai, Jing Zhou, Deqin Yang","doi":"10.1080/20002297.2026.2739029","DOIUrl":"https://doi.org/10.1080/20002297.2026.2739029","url":null,"abstract":"<p><strong>Background: </strong>The vacuolar-type H⁺-ATPases (V-ATPase) are a multi-subunit enzyme located in the <i>Candida albicans</i> (<i>C. albicans</i>) vacuolar membrane. It is responsible for ion transportation and modulates several cellular responses, with the key structure being the c ring in the V₀ domain. However, c ring's role in oral epithelial infection remains unclear.</p><p><strong>Objective: </strong>This study aimed to reveal the role of V₀c' subunit (encoding the gene <i>VMA11</i>), one of the subunits assemble c ring for oral Candidiasis <i>in vitro</i> and <i>in vivo</i>.</p><p><strong>Materials and methods: </strong><i>VMA11</i> deletion and complementation mutants were constructed to evaluate the oral epithelial infection abilities of V₀c' subunit, and western blotting and RT-qPCR were used to assess the regulate pathways for hyphal formation. Human oral mucosal keratinocyte (HOK) cells infected by <i>C. albicans</i> were subjected to transcriptome sequencing, and further validated using western blotting and immunofluorescence. Then, the impact of c ring specific inhibitor Bafilomycin A1 on oral epithelial infection by <i>C. albicans</i> were examined.</p><p><strong>Results: </strong>A <i>VMA11</i>-deleted mutant displayed reduced ATP contents, which decreased lower Ras and cyclic adenosine monophosphate (cAMP) levels, leading to inhibited hyphal growth by affecting the cAMP-protein kinase A (PKA) pathway. Additionally, the absence of <i>VMA11</i> further weakened <i>C. albicans</i> adhesion and damage capabilities toward oral epithelial cells, resulting in a diminished epithelial response associated with mitogen-activated protein kinase (MAPK) signaling. Bafilomycin A1 was then proved to effectively protect oral epithelium from <i>C. albicans</i> infection, which was consistent with the phenotypes of <i>VMA11</i> deletion mutant.</p><p><strong>Conclusion: </strong>Our findings highlight the crucial functions of V-ATPase V₀c' subunit in oral epithelial infection and suggest that targeting the V-ATPase c ring may be a promising antifungal strategy against oral candidiasis.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2739029"},"PeriodicalIF":6.7,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13629789/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149110758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic pairing of <i>Lactobacillus helveticus</i> Stellar Lh-170 and <i>Lactiplantibacillus plantarum</i> Stellar Lp-199 suppresses <i>Streptococcus mutans</i> and disrupts cariogenic biofilms.","authors":"Kris M James, Zack Klassen, Peter A Cadieux, Jeremy P Burton, Maxim D Seferovic","doi":"10.1080/20002297.2026.2723648","DOIUrl":"https://doi.org/10.1080/20002297.2026.2723648","url":null,"abstract":"<p><strong>Background: </strong><i>Streptococcus mutans</i> exists within a biofilm ecosystem where neighboring microbes influence each other's viability. Probiotic strains with complementary antagonistic properties may enhance suppression beyond individual strains. An evaluation of probiotic combinations assessed synergistic biofilm and pathogen suppression.</p><p><strong>Methods: </strong>Nineteen probiotic strains were screened for hydrogen peroxide production and antagonism of <i>Streptococcus mutans</i>; five candidates were further tested in two-strain combinations for synergistic antagonism of <i>S. mutans</i> and hydroxyapatite adhesion. An artificial saliva culture model assessed both the inhibition and disruption of <i>S. mutans</i> biofilms alone and with <i>Candida albicans</i>, with findings validated on human molars.</p><p><strong>Results: </strong>Of the 19 strains assessed, <i>Lactobacillus helveticus</i> Stellar^TM Lh-170 and <i>Lactiplantibacillus plantarum</i> Stellar^TM Lp-199 exhibited the strongest synergistic antagonism against all tested <i>S. mutans</i> strains (<i>p</i> < 0.001). Both strains rapidly produced H₂O₂ and adhered to hydroxyapatite. When co-incubated at a 10:1 ratio with S<i>. mutans</i>, the combination reduced biofilm biomass by 62% (n.s.) and significantly reduced <i>S. mutans</i> viability below detectable CFU limits (~10⁷-fold reduction). When co-incubated with <i>S. mutans</i> and <i>C. albicans</i> together, dose-dependent reductions in the viability of both pathogens were observed. When pre-established <i>S. mutans</i> biofilms were treated with the two-strain combination, the number of viable <i>S. mutans</i> CFUs was reduced by >  10,000-fold <i>in vitro</i> and 3,135-fold in extracted human molars after 24 h (<i>p</i> < 0.0001).</p><p><strong>Conclusions: </strong>Synergistic strain combinations effectively modulate cariogenic pathogens <i>in vitro, exceeding benchmark strains</i>. These findings support the clinical evaluation of the <i>Lactobacillus helveticus</i> Stellar Lh-170 and <i>Lactiplantibacillus plantarum</i> Stellar Lp-199 combination for prophylactic caries risk management.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2723648"},"PeriodicalIF":6.7,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13629822/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149110627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral microbiome-metabolome axis links glycerophospholipid dysregulation to Alzheimer's disease.","authors":"Mengqi Jia, Runjuan Yang, Ying Xu, Zhenghua Wu, Jin Lu, Zuan Li, Qiying Yan, Guorong Fan, Yaxing Gui","doi":"10.1080/20002297.2026.2732297","DOIUrl":"https://doi.org/10.1080/20002297.2026.2732297","url":null,"abstract":"<p><strong>Introduction: </strong>This study aimed to evaluate the feasibility of using tongue biofilm as a non-invasive, patient-friendly, and cost-effective biomarker source for identifying early microbial and metabolic disturbances associated with Alzheimer's disease (AD).</p><p><strong>Materials and methods: </strong>A total of 62 outpatients were enrolled (31 with AD and 31 cognitively normal controls). Tongue biofilm samples were analyzed using 16S rRNA sequencing and untargeted metabolomics via UPLC-Q/TOF-MS. Additionally, cerebrospinal fluid (CSF) samples from 36 individuals (18 per group) were examined under identical untargeted metabolomics processing protocols to validate the metabolomic findings.</p><p><strong>Results and discussion: </strong>Microbiome analysis revealed an increased relative abundance of Proteobacteria in the AD group, while Firmicutes and Bacteroidetes were enriched in controls. Metabolomic profiling identified 88 significantly different metabolites between groups, 64 of which achieved area under the curve (AUC) values > 0.90 in the ROC analysis. Glycerophospholipid metabolism has emerged as a key dysregulated pathway in AD, a finding further corroborated by CSF metabolomic analysis. Tongue biofilm represents a non-invasive, cost-effective, and accessible biomarker source, offering potential for AD diagnosis and research when coupled with microbiome and metabolomic analyses. Moreover, the role of glycerophospholipid metabolism in AD pathogenesis warrants further investigation.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2732297"},"PeriodicalIF":6.7,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13629805/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149110704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-FimA monoclonal antibodies reveal dual roles in porphyromonas gingivalis pathogenesis.","authors":"Mingya Cao, Yichang Gao, Siyu Wang, Chengye He, Jieyu Zhang, Min Yan, Shengke Zhou, Zihan Zhao, Yuanyuan Pan, Han Wang, Jingying Chen, Xia Li","doi":"10.1080/20002297.2026.2729552","DOIUrl":"https://doi.org/10.1080/20002297.2026.2729552","url":null,"abstract":"<p><strong>Background: </strong><i>Porphyromonas gingivalis</i> (<i>P. gingivalis</i>) is a keystone pathogen in periodontitis, a chronic inflammatory disease with systemic links. Its major fimbrial protein, FimA, is essential for bacterial adhesion and co‑aggregation, making it a promising immunotherapeutic target. Although anti-FimA antibodies are detected in patients and correlate with disease severity, their functional roles remain incompletely understood.</p><p><strong>Objective: </strong>To generate and functionally characterize FimA-targeting monoclonal antibodies (mAbs) and define their functional impact on <i>P. gingivalis</i> pathogenesis.</p><p><strong>Design: </strong>Mice were immunized with recombinant FimA (rFimA) protein to generate hybridomas producing high-affinity mAbs that recognize distinct, non-overlapping epitopes. The functional effects of these mAbs on <i>P. gingivalis</i> adhesion, phagocytosis, complement killing, aggregation and biofilm architecture were evaluated using binding assays, flow cytometry, biolayer interferometry and microscopy.</p><p><strong>Results: </strong>A panel of high-affinity anti-FimA mAbs targeting distinct, non-overlapping epitopes was generated. Although all mAbs blocked bacterial adhesion and enhanced phagocytosis, they showed limited complement-dependent killing and, paradoxically, induced aggregation that promoted structured biofilm formation.</p><p><strong>Conclusion: </strong>Anti-FimA mAbs exert a dual and opposing functionality, conferring host protection while paradoxically enhancing bacterial persistence through biofilm promotion. These findings reveal a previously unrecognized complexity in anti-FimA humoral immunity and provide important insights for the design of targeted immunotherapies against <i>P. gingivalis</i> infection.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2729552"},"PeriodicalIF":6.7,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13629768/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149110581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}