Journal of Oral Microbiology最新文献

Oral administration of Porphyromonas gingivalis to mice with diet-induced obesity impairs cognitive function associated with microglial activation in the brain. 饮食诱发肥胖的小鼠口服牙龈卟啉单胞菌会损害与大脑小胶质细胞活化相关的认知功能。

IF 3.7 2区医学

Journal of Oral Microbiology Pub Date : 2024-11-14 eCollection Date: 2024-01-01 DOI: 10.1080/20002297.2024.2419155

Kana Oue, Yosuke Yamawaki, Kazuhisa Ouhara, Eiji Imado, Tetsuya Tamura, Mitsuru Doi, Yoshitaka Shimizu, Mitsuhiro Yoshida, Noriyoshi Mizuno, Norimitsu Morioka, Takashi Kanematsu, Masahiro Irifune, Yukio Ago

{"title":"Oral administration of Porphyromonas gingivalis to mice with diet-induced obesity impairs cognitive function associated with microglial activation in the brain.","authors":"Kana Oue, Yosuke Yamawaki, Kazuhisa Ouhara, Eiji Imado, Tetsuya Tamura, Mitsuru Doi, Yoshitaka Shimizu, Mitsuhiro Yoshida, Noriyoshi Mizuno, Norimitsu Morioka, Takashi Kanematsu, Masahiro Irifune, Yukio Ago","doi":"10.1080/20002297.2024.2419155","DOIUrl":"10.1080/20002297.2024.2419155","url":null,"abstract":"Objective: Both periodontal disease and obesity are risk factors for dementia, but their links to 1brain function remain unclear. In this study, we examined the effects of oral infection with a periodontal pathogen on cognitive function in a mouse model of obesity, focusing on the roles of microglia.Methods: To create a mouse model of diet-induced obesity and periodontitis, male C57BL/6 J mice were first fed a high-fat diet containing 60% lipid calories for 18 weeks, beginning at 12 weeks of age, to achieve diet-induced obesity. Then, Porphyromonas gingivalis administration in the oral cavity twice weekly for 6 weeks was performed to induce periodontitis in obese mice.Results: Obese mice orally exposed to P. gingivalis showed cognitive impairment in the novel object recognition test. Increased expression levels of inflammatory cytokines (e.g. interleukin-1β and tumor necrosis factor-α) were observed in the hippocampus of P. gingivalis-treated obese mice. Immunohistochemical analysis revealed that microglia cell body size was increased in the hippocampus and prefrontal cortex of P. gingivalis-treated obese mice, indicating microglial activation. Furthermore, depletion of microglia by PLX3397, a colony-stimulating factor 1 receptor inhibitor, ameliorated cognitive dysfunction.Conclusion: These results suggest that microglia mediate periodontal infection-induced cognitive dysfunction in obesity.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"16 1","pages":"2419155"},"PeriodicalIF":3.7,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11565673/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142647359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RE: colonization of bacterial and viral respiratory pathogens among healthcare workers in China during COVID-19 pandemic. RE: COVID-19 大流行期间中国医护人员呼吸道病原体的细菌和病毒定植。

IF 3.7 2区医学

Journal of Oral Microbiology Pub Date : 2024-11-11 eCollection Date: 2024-01-01 DOI: 10.1080/20002297.2024.2427698

Hineptch Daungsupawong, Viroj Wiwanitkit

引用次数: 0

In vitro plaque formation model to unravel biofilm formation dynamics on implant abutment surfaces. 体外菌斑形成模型，揭示种植体基台表面生物膜形成动力学。

IF 3.7 2区医学

Journal of Oral Microbiology Pub Date : 2024-11-08 eCollection Date: 2024-01-01 DOI: 10.1080/20002297.2024.2424227

Romualdus Nugraha Catur Utomo, Alena Lisa Palkowitz, Lin Gan, Anna Rudzinski, Julia Franzen, Hendrik Ballerstedt, Martin Zimmermann, Lars Mathias Blank, Horst Fischer, Stefan Wolfart, Taskin Tuna

{"title":"In vitro plaque formation model to unravel biofilm formation dynamics on implant abutment surfaces.","authors":"Romualdus Nugraha Catur Utomo, Alena Lisa Palkowitz, Lin Gan, Anna Rudzinski, Julia Franzen, Hendrik Ballerstedt, Martin Zimmermann, Lars Mathias Blank, Horst Fischer, Stefan Wolfart, Taskin Tuna","doi":"10.1080/20002297.2024.2424227","DOIUrl":"https://doi.org/10.1080/20002297.2024.2424227","url":null,"abstract":"Background: Biofilm formation on implant-abutment surfaces can cause inflammatory reactions. Ethical concerns often limit intraoral testing, necessitating preliminary in vitro or animal studies. Here, we propose an in vitro model using human saliva and hypothesize that this model has the potential to closely mimic the dynamics of biofilm formation on implant-abutment material surfaces in vivo.Methods: A saliva stock was mixed with modified Brain-Heart-Infusion medium to form biofilms on Titanium-Aluminum-Vanadium (Ti6Al4V) and Yttria-partially Stabilized Zirconia (Y-TZP) discs in 24-well plates. Biofilm analyses included crystal violet staining, intact cell quantification with BactoBox, 16S rRNA gene analysis, and short-chain fatty acids measurement. As a control, discs were worn in maxillary splints by four subjects for four days to induce in vivo biofilm formation.Results: After four days, biofilms fully covered Ti6Al4V and Y-TZP discs both in vivo and in vitro, with similar cell viability. There was a 60.31% overlap of genera between in vitro and in vivo biofilms in the early stages, and 41% in the late stages. Ten key oral bacteria, including Streptococcus, Haemophilus, Neisseria, Veillonella, and Porphyromonas, were still detectable in vitro, representing the common stages of oral biofilm formation.Conclusion: This in vitro model effectively simulates oral conditions and provides valuable insights into biofilm dynamics.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"16 1","pages":"2424227"},"PeriodicalIF":3.7,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11552293/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142622283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of a Burkholderia ambifaria strain from plants as a novel promising probiotic in dental caries management. 将植物中的伯克霍尔德氏菌（Burkholderia ambifaria）菌株作为一种新型益生菌用于龋齿防治的评估。

IF 3.7 2区医学

Journal of Oral Microbiology Pub Date : 2024-11-03 eCollection Date: 2024-01-01 DOI: 10.1080/20002297.2024.2420612

Zirang Chen, Yangyu Lu, Zhezhen Xu, Lijing Wu, Xi Wei, Yanling Cai

{"title":"Evaluation of a Burkholderia ambifaria strain from plants as a novel promising probiotic in dental caries management.","authors":"Zirang Chen, Yangyu Lu, Zhezhen Xu, Lijing Wu, Xi Wei, Yanling Cai","doi":"10.1080/20002297.2024.2420612","DOIUrl":"10.1080/20002297.2024.2420612","url":null,"abstract":"Background: Probiotics serve as a novel preventive or therapeutic approach for dental caries owing to their ability to reverse dysbiosis and restore a healthy microbiota. Here, we identified Burkholderia ambifaria AFS098024 as a probiotic candidate isolated from plants.Methods: The safety of B. ambifaria was evaluated by hemolytic activity, D-lactic acid production and antibiotic susceptibility. In vitro biofilm model derived from the saliva of caries-free and caries-active donors and in vivo rat caries model were used to assess the efficacy of B. ambifaria in caries prevention and treatment.Results: B. ambifaria was safe as a probiotic candidate and it could integrate with in vitro biofilm model. It significantly reduced the biomass and lactate production of biofilms from caries-active donors and disrupted biofilm structures. B. ambifaria effectively reduced the severity of carious lesions in rat molars, regardless of the inoculation sequence. Molars pretreated or treated with B. ambifaria demonstrated notably higher enamel volumes. Additionally, colonization of rat molars by B. ambifaria persisted for 6 weeks.Conclusion: The B. ambifaria strain used in this study holds promise as a probiotic for inhibiting dental caries, both in vitro and in vivo.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"16 1","pages":"2420612"},"PeriodicalIF":3.7,"publicationDate":"2024-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11536693/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142583243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methanobrevibacter oralis: a comprehensive review. 口腔甲烷杆菌：综述。

IF 3.7 2区医学

Journal of Oral Microbiology Pub Date : 2024-11-01 eCollection Date: 2024-01-01 DOI: 10.1080/20002297.2024.2415734

Virginie Pilliol, Boualam Mahmoud Abdelwadoud, Hamiech Aïcha, Tellissi Lucille, Aboudharam Gérard, Tassery Hervé, Drancourt Michel, Grine Ghiles, Terrer Elodie

{"title":"Methanobrevibacter oralis: a comprehensive review.","authors":"Virginie Pilliol, Boualam Mahmoud Abdelwadoud, Hamiech Aïcha, Tellissi Lucille, Aboudharam Gérard, Tassery Hervé, Drancourt Michel, Grine Ghiles, Terrer Elodie","doi":"10.1080/20002297.2024.2415734","DOIUrl":"10.1080/20002297.2024.2415734","url":null,"abstract":"Methanobrevibacter oralis (M. oralis) has predominated human oral microbiota methanogenic archaea as far back as the Palaeolithic era in Neanderthal populations and gained dominance from the 18th century onwards. M. oralis was initially isolated from dental plaque samples collected from two apparently healthy individuals allowing its first characterization. The culture of M. oralis is fastidious and has been the subject of several studies to improve its laboratory growth. Various PCR methods are used to identify M. oralis, targeting either the 16S rRNA gene or the mcrA gene. However, only one RTQ-PCR system, based on a chaperonin gene, offers specificity, and allows for microbial load quantification. Next-generation sequencing contributed five draft genomes, each approximately 2.08 Mb (±0.052 Mb) with a 27.82 (±0.104) average GC%, and two ancient metagenomic assembled genomes. M. oralis was then detected in various oral cavity sites in healthy individuals and those diagnosed with oral pathologies, notably periodontal diseases, and endodontic infections. Transmission pathways, possibly involving maternal milk and breastfeeding, remain to be clarified. M. oralis was further detected in brain abscesses and respiratory tract samples, bringing its clinical significance into question. This review summarizes the current knowledge about M. oralis, emphasizing its prevalence, associations with dysbiosis and pathologies in oral and extra-oral situations, and symbiotic relationships, with the aim of paving the way for further investigations.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"16 1","pages":"2415734"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11536694/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142583241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alterations of oral microbiome and metabolic signatures and their interaction in oral lichen planus. 口腔扁平苔藓中口腔微生物组和代谢特征的改变及其相互作用。

IF 3.7 2区医学

Journal of Oral Microbiology Pub Date : 2024-10-30 eCollection Date: 2024-01-01 DOI: 10.1080/20002297.2024.2422164

Li Yan, Jingyi Xu, Fangzhi Lou, Yunmei Dong, Shiping Lv, Ning Kang, Zhuoyan Luo, Yiyun Liu, Juncai Pu, Xiaogang Zhong, Ping Ji, Peng Xie, Xin Jin

{"title":"Alterations of oral microbiome and metabolic signatures and their interaction in oral lichen planus.","authors":"Li Yan, Jingyi Xu, Fangzhi Lou, Yunmei Dong, Shiping Lv, Ning Kang, Zhuoyan Luo, Yiyun Liu, Juncai Pu, Xiaogang Zhong, Ping Ji, Peng Xie, Xin Jin","doi":"10.1080/20002297.2024.2422164","DOIUrl":"10.1080/20002297.2024.2422164","url":null,"abstract":"Background: Oral lichen planus (OLP) is a chronic oral mucosal inflammatory disease with a risk of becoming malignant. Emerging evidence suggests that microbial imbalance plays an important role in the development of OLP. However, the association between the oral microbiota and the metabolic features in OLP is still unclear.Methods: We conducted 16S rRNA sequencing and metabolomics profiling on 95 OLP patients and 105 healthy controls (HC).To study oral microbes and metabolic changes in OLP, we applied differential analysis, Spearman correlation analysis and four machine learning algoeithms.Results: The alpha and beta diversity both differed between OLP and HC. After adjustment for gender and age, we found an increase in the relative abundance of Pseudomonas, Aggregatibacter, Campylobacter, and Lautropia in OLP, while 18 genera decreased in OLP. A total of 153 saliva metabolites distinguishing OLP from HC were identified. Notably, correlations were found between Oribacterium, specific lipid and amino acid metabolites, and OLP's clinical phenotype. Additionally, the combination of Pseudomonas, Rhodococcus and (±)10-HDoHE effectively distinguished OLP from HC.Conclusions: Based on multi-omics data, this study provides comprehensive evidence of a novel interplay between oral microbiome and metabolome in OLP pathogenesis using the oral microbiota and metabolites of OLP patients.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"16 1","pages":"2422164"},"PeriodicalIF":3.7,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11533246/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142576259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of Lactobacillus plantarum in oral health: a review of current studies. 植物乳杆菌在口腔健康中的作用：当前研究综述。

IF 3.7 2区医学

Journal of Oral Microbiology Pub Date : 2024-10-22 eCollection Date: 2024-01-01 DOI: 10.1080/20002297.2024.2411815

Xinyan Huang, Jianhang Bao, Mingzhen Yang, Yingying Li, Youwen Liu, Yuankun Zhai

{"title":"The role of Lactobacillus plantarum in oral health: a review of current studies.","authors":"Xinyan Huang, Jianhang Bao, Mingzhen Yang, Yingying Li, Youwen Liu, Yuankun Zhai","doi":"10.1080/20002297.2024.2411815","DOIUrl":"https://doi.org/10.1080/20002297.2024.2411815","url":null,"abstract":"Background: Oral non-communicable diseases, particularly dental caries and periodontal disease, impose a significant global health burden. The underlying microbial dysbiosis is a prominent factor, driving interest in strategies that promote a balanced oral microbiome. Lactobacillus plantarum, a gram-positive lactic acid bacterium known for its adaptability, has gained attention for its potential to enhance oral health. Recent studies have explored the use of probiotic L. plantarum in managing dental caries, periodontal disease, and apical periodontitis. However, a comprehensive review on its effects in this context is still lacking.Aims: This narrative review evaluates current literature on L. plantarum's role in promoting oral health and highlights areas for future research.Content: In general, the utilization of L. plantarum in managing non-communicable biofilm-dependent oral diseases is promising, but additional investigations are warranted. Key areas for future study include: exploring its mechanisms of action, identifying optimal strains or strain combinations of L. plantarum, determining effective delivery methods and dosages, developing commercial antibacterial agents from L. plantarum, and addressing safety considerations related to its use in oral care.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"16 1","pages":"2411815"},"PeriodicalIF":3.7,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11497578/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Findings and methodologies in oral phageome research: a systematic review. 口腔噬菌体研究的结果和方法：系统综述。

IF 3.7 2区医学

Journal of Oral Microbiology Pub Date : 2024-10-16 eCollection Date: 2024-01-01 DOI: 10.1080/20002297.2024.2417099

Xin Chen, Ting Zou, Guicong Ding, Shan Jiang

{"title":"Findings and methodologies in oral phageome research: a systematic review.","authors":"Xin Chen, Ting Zou, Guicong Ding, Shan Jiang","doi":"10.1080/20002297.2024.2417099","DOIUrl":"https://doi.org/10.1080/20002297.2024.2417099","url":null,"abstract":"Background: The oral microbiome serves as both an indicator and a mediator of oral health. Evidence indicates that bacteriophages (phages) are widely present in the oral microbiome and exhibit diverse classifications and interactions with human cells and other microbes. These phages constitute the oral phageome, which potentially exerts significant yet unexplored effects on the interplay between oral and general health.Methods: Three databases (PubMed/MEDLINE, Embase and Scopus) were searched for metagenomic analyses that investigated the oral phageome. Eligible studies were synthesized based on their methodological approaches and findings.Results: A total of 14 articles were included in this systematic review. Among the 14 articles included, there were six studies that discussed disease-related alterations, along with a discursive examination of additional variables such as sampling niches, external interventions and methodologies. The phages that infect Streptococcus Actinomyces Haemophilus, and Veillonella have been discovered to be associated with chronic periodontitis, caries, and pancreatic ductal carcinoma.Conclusions: This systematic review focuses on findings and methodologies in oral phageome studies, which were conducted using highly heterogeneous methodologies that explored the oral phageome in multiple directions while placing constraints on quantitative statistics. Combining different kinds of sample types, utilizing the characteristics of different methods, involving both DNA and RNA phages, and differentiating lysogenic and lytic phages should be the distinction of further studies.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"16 1","pages":"2417099"},"PeriodicalIF":3.7,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11485842/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142468024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibitory effect of helium cold atmospheric plasma on cariogenic biofilms 氦冷等离子体对致癌生物膜的抑制作用

IF 4.5 2区医学

Journal of Oral Microbiology Pub Date : 2024-09-09 DOI: 10.1080/20002297.2024.2397831

Leandro Wagner Figueira, Ana Bessa Muniz, Anelise Cristina Osorio Cesar Doria, Thalita Mayumi Castaldelli Nishime, Konstantin Georgiev Kostov, Cristiane Y. Koga-Ito

引用次数: 0

Correction. 更正。

IF 3.7 2区医学

Journal of Oral Microbiology Pub Date : 2024-08-20 eCollection Date: 2024-01-01 DOI: 10.1080/20002297.2024.2393043

引用次数: 0