Journal of Oral Microbiology最新文献

{"title":"DNA virus tropism in healthy dental pulp: an in-situ reservoir site for torque teno virus and parvovirus B19.","authors":"O I Mielonen, S Hänninen, J Willberg, T Salo, M Mauramo","doi":"10.1080/20002297.2025.2499924","DOIUrl":"https://doi.org/10.1080/20002297.2025.2499924","url":null,"abstract":"<p><strong>Background: </strong>The presence of viruses in healthy teeth has not been extensively studied, although some viral traces have been detected in both healthy and diseased dental pulps in previous studies focusing primarily on a single species. The aim of this study is to clarify the persistence of DNA viruses in dental tissues and their impact on tissue composition.</p><p><strong>Materials and methods: </strong>Here, the prevalence of persistent DNA viruses in intact third molars (<i>n</i> = 17) was assessed via quantitative PCR to detect human parvovirus B19 (B19V), torque teno virus (TTV) and nine human herpesviruses. Also, H&E-stained tissue sections of the samples were analyzed for any potential inflammatory process. RNAscope in-situ hybridization was performed for B19V, TTV and HHV7 subsequently.</p><p><strong>Results: </strong>Viral DNA of five different viruses was detected in 5 of the 17 samples (29.4%) including B19V (<i>n</i> = 2), TTV (<i>n</i> = 2), HHV7 (<i>n</i> = 2), HCMV (<i>n</i> = 1) or EBV (<i>n</i> = 1) in dental pulps with no signs of cytopathic effect, inflammatory cell accumulations or necrosis. RNAscope in-situ hybridization confirmed the presence of B19V and TTV in non-inflamed pulp tissue.</p><p><strong>Conclusions: </strong>These findings emphasized that even in the absence of a disease evaluated by histology, dental pulp can harbor DNA viruses and be an anatomical site of virus tropism, suggesting viral persistence rather than direct pathogenic activity.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2499924"},"PeriodicalIF":3.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12051552/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144004426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the salivary microbiome of adults with inflammatory bowel disease.","authors":"Vanessa DeClercq, Robyn J Wright, Johan van Limbergen, Morgan G I Langille","doi":"10.1080/20002297.2025.2499923","DOIUrl":"https://doi.org/10.1080/20002297.2025.2499923","url":null,"abstract":"<p><strong>Background: </strong>Perturbations of the gut microbiota in patients with inflammatory bowel disease (IBD) have been extensively characterised, but changes to the oral microbiome remain understudied. This study aimed to evaluate the oral microbiome of adults with IBD and of matched controls.</p><p><strong>Methods: </strong>Saliva samples and data were obtained from a Canadian population cohort (<i>n</i> = 320). The salivary microbiome was characterised using 16S rRNA gene sequencing and examined for differences between control participants and those with IBD, as well as disease subcategories (Crohn's Disease and Ulcerative Colitis).</p><p><strong>Results: </strong>Alpha diversity was significantly lower in participants with IBD than controls in unadjusted models and many remained significant after adjusting for covariates. Significant differences in some beta diversity metrics between participants with IBD and controls were found, although these did not remain significant when adjusted for covariates. Ten genera were significantly differentially abundant between cases and controls. <i>Veillonella</i> and <i>Streptococcus</i> were both increased in abundance in IBD cases <i>vs</i> controls (25% <i>vs</i> 22% and 14% <i>vs</i> 12%, respectively).</p><p><strong>Conclusion: </strong>These results showcase changes in oral microbial diversity and composition in those living with IBD and highlight the potential of using the salivary microbiome as a biomarker for screening or monitoring IBD.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2499923"},"PeriodicalIF":3.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12046613/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of oral microbiome composition in elderly Chinese patients with hypertension: a cross-sectional study.","authors":"Xin Chen, Wen-Yong Lin, Feng-Wei Zhang, Li-Qiang Guo, Han Ge, Ding-Zuo Ge, Juan-Juan Tan, Bao-Cheng Liu, Rui-Rui Wang, Lei Zhang","doi":"10.1080/20002297.2025.2489603","DOIUrl":"https://doi.org/10.1080/20002297.2025.2489603","url":null,"abstract":"<p><strong>Background: </strong>Hypertension is a prevalent metabolic disorder in the elderly, with its pathogenesis linked to gut microbiota dysbiosis. Recent studies suggested that oral microbiota may also play a role in hypertension development, yet its relationship with hypertension in the elderly remains underexplored.</p><p><strong>Objective: </strong>This cross-sectional study aimed to examine the structure of the oral microbiota and its association with hypertension in elderly patients, providing insights into hypertension prevention and treatment.</p><p><strong>Methods: </strong>A total of 206 subjects (60-89 years) were categorized into normal (CON) and hypertensive (HTN) groups, based on the Chinese Hypertension Guidelines. Saliva samples were analyzed using 16S rRNA gene sequencing.</p><p><strong>Results: </strong>Oral microbiota composition was significantly influenced by blood pressure. At the phylum level, Synergistetes and Spirochaetes were more significantly abundant in the HTN group, while at the genus level Treponema and Leptothrix was higher, Actinomyces and Capnocytophaga were lower in HTN. Random Forest analysis identified 15 key microbiota as strong discriminators of HTN (AUC 0.74). Blood pressure was negatively correlated with Actinomycetes and positively correlated with Leptothrix. PICRUST2 analysis revealed elevated chlorinated compound degradation in HTN patients.</p><p><strong>Conclusions: </strong>This study identified distinct oral microbiota in elderly hypertensive patients, highlighting the role of the oral microbiome in hypertension pathogenesis.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2489603"},"PeriodicalIF":3.7,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12016255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144002668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Candida albicans</i> impacts carbohydrate metabolism of <i>Enterococcus faecalis</i> in interkingdom biofilms.","authors":"Nicole de Mello Fiallos, Iriana J Zanin Dos Santos, Debra N Brunson, Jessica K Kajfasz, Lin Zeng, Rossana de Aguiar Cordeiro, José A Lemos, Jacqueline Abranches","doi":"10.1080/20002297.2025.2492194","DOIUrl":"https://doi.org/10.1080/20002297.2025.2492194","url":null,"abstract":"<p><strong>Objectives: </strong>This study investigated the transcriptional and physiological responses of <i>Enterococcus faecalis</i>, an opportunistic pathogen linked to endodontic infections, when cultivated in dual-species biofilms with <i>Candida albicans</i>, a yeast pathobiont found in the oral cavity.</p><p><strong>Methods: </strong>Forty-eight-hour <i>E. faecalis</i> OG1RF biofilms were developed in BHI medium as mono- or dual-species with <i>C. albicans</i> SC5314. Biofilms were assessed for biomass, colony-forming units (CFUs), and architecture using confocal microscopy. RNA sequencing was performed on an Illumina platform. Mannose-PTS activity and glycerol quantification assays were conducted to investigate changes in carbohydrate metabolism.</p><p><strong>Results: </strong>Transcriptomic analysis revealed 149 <i>E. faecalis</i> genes differentially expressed in dual-species biofilms. Genes linked to mannose-PTS and glycerol metabolism were notably upregulated. Mannose-PTS activity was significantly higher in dual-species biofilms. Mannose, as the sole carbohydrate source, increased <i>E. faecalis</i> CFUs and decreased <i>C. albicans</i> CFUs in co-culture, while glucose had no effect. As <i>C. albicans</i> is a glycerol net producer, glycerol levels were always higher when <i>C. albicans</i> was present, likely contributing to the upregulation of glycerol metabolism genes in <i>E. faecalis</i> when in co-cultures.</p><p><strong>Conclusions: </strong>The presence of <i>C. albicans</i> alters <i>E. faecalis</i> gene expression and metabolism, suggesting metabolic crosstalk that may influence their pathogenicity and role in oral infections.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2492194"},"PeriodicalIF":3.7,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12004723/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144012620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary analysis of salivary microbiota in catathrenia (nocturnal groaning) using machine learning algorithms.","authors":"Min Yu, Yujia Lu, Wanxin Zhang, Xu Gong, Zeliang Hao, Liyue Xu, Yongfei Wen, Xiaosong Dong, Fang Han, Xuemei Gao","doi":"10.1080/20002297.2025.2489613","DOIUrl":"https://doi.org/10.1080/20002297.2025.2489613","url":null,"abstract":"<p><strong>Objectives: </strong>The present study aimed to characterize the salivary microbiota in patients with catathrenia and to longitudinally validate potential biomarkers after treatment with mandibular advancement devices (MAD).</p><p><strong>Materials and methods: </strong>Twenty-two patients with catathrenia (12 M/10 F, median age 28 y) and 22 age-matched control volunteers (8 M/14 F, median age 30 y) were included in the cross-sectional study. Video/audio polysomnography was conducted for diagnosis. All patients received treatment with custom-fit MAD and were followed for one month. Ten patients (6 M/4 F) underwent post-treatment PSG. Salivary samples were collected, and microbial characteristics were analyzed using 16S rRNA gene sequencing. The 10-fold cross-validated XGBoost and nested Random Forest Classifier machine learning algorithms were utilized to identify potential biomarkers.</p><p><strong>Results: </strong>In the cross-sectional study, patients with catathrenia had lower α-diversity represented by Chao 1, Faith's phylogenetic diversity (pd), and observed species. Beta-diversity based on the Bray-Curtis dissimilarities revealed a significant inter-group separation (<i>p</i> = 0.001). The inter-group microbiota distribution was significantly different on the phylum and family levels. The treatment of MAD did not alter salivary microbiota distribution significantly. Among the most important genera in catathrenia and control classification identified by machine learning algorithms, four genera, <i>Alloprevotella, Peptostreptococcaceae_XI_G1, Actinomyces</i> and <i>Rothia</i>, changed significantly with MAD treatment. Correlation analysis revealed that <i>Alloprevotella</i> was negatively related to the severity of catathrenia (r²= -0.63, <i>p</i> < 0.001).</p><p><strong>Conclusions: </strong>High-throughput sequencing revealed that the salivary microbiota composition was significantly altered in patients with catathrenia. Some characteristic genera (<i>Alloprevotella, Peptostreptococcaceae_XI_G1, Actinomyces,</i> and <i>Rothia</i>) could be potential biomarkers sensitive to treatment. Future studies are needed to confirm and determine the mechanisms underlying these findings.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2489613"},"PeriodicalIF":3.7,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12004722/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Odontogenic brain abscess caused by <i>Porphyromonas gingivalis</i> and <i>Streptococcus constellatus</i>: a case report and review article.","authors":"Siyu Sun, Rui He, Shan Chen, Jing Ren, Xinrong Ma, Junying Yang","doi":"10.1080/20002297.2025.2485197","DOIUrl":"https://doi.org/10.1080/20002297.2025.2485197","url":null,"abstract":"<p><strong>Background: </strong>Odontogenic brain abscess is a rare, but potentially fatal, central nervous system infection, with insidious onset and unclear etiology.</p><p><strong>Methods: </strong>This case reports a 70-year-old male patient who developed an odontogenic brain abscess secondary to periodontal infection and underwent neurological surgery. Extract pus during surgery for the metagenomic next-generation sequencing (mNGS).</p><p><strong>Results: </strong>The mNGS of pus samples obtained from brain abscess aspiration identified the periodontal pathogens <i>Porphyromonas gingivalis</i> and <i>Streptococcus constellatus</i>. Consequently, he was referred to the department of stomatology for further examination and treatment.</p><p><strong>Conclusions: </strong>Our study found that major periodontal pathogens including <i>P. gingivalis</i> and <i>S. constellatus</i> were essential in the development of odontogenic brain abscesses; thus, timely intervention and preventive measures are important for treatment.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2485197"},"PeriodicalIF":3.7,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12001842/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144000152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interactions between non-<i>albicans Candida</i> and <i>Streptococcus mutans</i> enhance cariogenic potential of dual-species interkingdom biofilms.","authors":"Wirunphat Sansanoa, Oranart Matangkasombut, Metanee Sangpitak, Lakshman Samaranayake, Panida Thanyasrisung","doi":"10.1080/20002297.2025.2492198","DOIUrl":"https://doi.org/10.1080/20002297.2025.2492198","url":null,"abstract":"<p><strong>Background: </strong>Non-<i>albicans Candida</i> species (NACs) are commonly found in carious lesions, yet their specific role in caries progression remains unclear. Hence, we conducted an <i>in</i> <i>vitro</i> study to explore how NACs interactions with <i>Streptococcus mutans</i> affect cariogenicity.</p><p><strong>Materials and methods: </strong>Dual-species interkingdom biofilms were developed with <i>S.mutans</i> and six <i>Candida</i> species, <i>C.albicans</i> and the NACs: <i>C.dubliniensis</i>, <i>C.glabrata</i>, <i>C.krusei</i>, <i>C.parapsilosis</i> and <i>C.tropicalis</i>. Biofilm mass, viable cell counts, and pH were evaluated in mono- and dual-species biofilms. Quantitative RT-PCR was used to assess the expression of <i>S.mutans</i> genes associated with cariogenicity.</p><p><strong>Results: </strong>Co-culturing <i>S.mutans</i> with either <i>C.albicans</i>, <i>C.glabrata</i>, or <i>C.tropicalis</i> significantly increased biofilm mass. While <i>S.mutans</i> numbers either increased or remained stable in dual-species biofilms, <i>C.krusei</i>, <i>C.parapsilosis</i>, and in particular <i>C.tropicalis</i> numbers significantly increased. All dual-species biofilms exhibited a pH below the critical demineralization level of enamel, akin to <i>S.mutans</i> mono-species biofilms. The expression of a battery of cariogenic genes in <i>S.mutans</i> was upregulated, particularly in dual-species biofilms with <i>C.krusei</i> and with <i>C.tropicalis</i>.</p><p><strong>Conclusion: </strong>NACs influence the biofilm production and the cariogenic gene expression of <i>S.mutans.</i> The dual-species biofilm of <i>S.mutans</i> and NACs, particularly <i>C.tropicalis</i>, likely possess heightened cariogenic potential. Further research is warranted to unravel these intriguing interactions within interkingdom biofilms.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2492198"},"PeriodicalIF":3.7,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11998306/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144002519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitrate reduction by salivary bacteria, glucose metabolism, and lifestyle.","authors":"Evangelia Morou-Bermúdez, Kai Guo, Jairelisse Morales Morales, Karina Ricart, Rakesh P Patel, José C Clemente, Kaumudi Joshipura","doi":"10.1080/20002297.2025.2489612","DOIUrl":"https://doi.org/10.1080/20002297.2025.2489612","url":null,"abstract":"<p><strong>Background: </strong>Nitrate reductases (NR) expressed in oral bacteria reduce nitrate to nitrite. Depending on the environmental conditions and types of bacteria present nitrite can be further reduced to ammonium via Dissimilatory Nitrate Reduction to Ammonium (DNRA), or alternatively to nitric oxide (NO), which impacts cardiometabolic health.</p><p><strong>Objective: </strong>To evaluate the associations between nitrate reduction by salivary bacteria, clinical markers of glucose metabolism, and lifestyle factors that can modulate the oral environment, potentially impacting DNRA and NR expression.</p><p><strong>Methods: </strong>A cross-sectional study was conducted using a convenience sample of 144 participants from the San Juan Overweight Adult Longitudinal Study (SOALS), which includes data on glucose metabolism and lifestyle. DNRA and NR activities were measured in saliva under aerobic or CO₂-enriched conditions.</p><p><strong>Results: </strong>DNRA activity was inversely associated with insulin resistance (HOMA-IR) [aerobic_{3rd vs.1st tertile}: β=-0.48 (-0.81, -0.15); CO₂-enriched_{3rd vs.1st tertile} β=-0.42 (-0.68, -0.17)], fasting blood glucose [aerobic_{3rd vs.1st tertile} β=-0.144 (-0.268, -0.019); CO₂-enriched_{3rd vs.1st tertile}: β=-0.070 (-0.130, -0.011)], and 2-h glucose [CO₂-enriched_{3rd vs.1st tertile}β=-0.21 (-0.37, -0.04)]. Current smokers had lower DNRA activity than non-smokers under aerobic conditions [β=-1.55 (-2.96, -0.14)], but higher under CO₂-enriched conditions [β = 0.93 (0.15, 1.71)]. Toothbrushing frequency (twice/day vs. once/day) was positively associated with DNRA activity under CO₂-enriched conditions [β = 4.11 (1.90, 6.32)] and with aerobic NR activity [β = 1.20, (0.14, 2.27)]. Physical activity was inversely associated with aerobic NR [β=-0.01, (-0.022, -0.003)]. Under CO₂-enriched conditions NR was inversely associated with the BMI (β=-0.11, <i>p</i> = 0.007). Aerobic NR was higher when sucrose was added to the assays (NADP vs. sucrose β=-0.74, <i>p</i> = 0.02) and positively associated with salivary nitrate levels (β = 0.002, <i>p</i> = 0.002).</p><p><strong>Conclusions: </strong>Nitrate reduction by salivary bacteria is inversely associated with insulin resistance and can be modulated by lifestyle factors. This knowledge could lead to the development of novel, non-invasive approaches for monitoring and preventing diabetes progression.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2489612"},"PeriodicalIF":3.7,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11986870/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling salivary microbiota diversity following kidney transplantation: insights from baseline peripheral blood lymphocyte subsets.","authors":"Xuyu Xiang, Tianyin Wang, Peng Ding, Yi Zhu, Ke Cheng, Yingzi Ming","doi":"10.1080/20002297.2025.2490284","DOIUrl":"https://doi.org/10.1080/20002297.2025.2490284","url":null,"abstract":"<p><strong>Background: </strong>Effective biomarkers are urgently needed to monitor immune suppression in kidney transplantation (KT) recipients. Our study identified a close association between the salivary microbiota and immunosuppressant concentrations. It is therefore hypothesized that the salivary microbiota may be linked to immune function.</p><p><strong>Materials and methods: </strong>We analyzed 108 saliva samples from 37 KT patients using 16S rRNA sequencing. Patients were clustered via K-means based on peripheral blood lymphocyte subset (PBLS) counts.</p><p><strong>Results: </strong>Cluster1 exhibited significantly higher CD4⁺ T cells (<i>p</i> < 0.0001), CD8⁺ T cells (<i>p</i> < 0.0001), and B cells (<i>p</i> = 0.0071) versus Cluster2, with marginally NK cells (<i>p</i> = 0.2319). Beta diversity indicated significant differences in microbial communities. LEfSe analysis identified 34 differential taxa at the genus level. A random forest model in a fivefold three-times repeated cross-validation, developed with differential taxa, discriminated patient groups well (AUC, 75.61% ± 14.54%), with Pseudopropionibacterium most contributing. Meanwhile, only Pseudopropionibacterium correlated with more than 2 PBLSs. Cluster2 was predicted to exhibit more primary and secondary bile acid synthesis, with differential expression of related enzymes.</p><p><strong>Conclusion: </strong>The absolute count of PBLSs is correlated with the composition of the salivary microbiota, with the strongest association observed between Pseudopropionibacterium and lymphocytes. Our study provides novel insights into immune monitoring post-KT.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2490284"},"PeriodicalIF":3.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11983535/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glycosylation of oral bacteria in modulating adhesion and biofilm formation.","authors":"Simeng Yi, Yingyu Liu, Qinrui Wu, Danning Zhao, Zhengyi Li, Xian Peng, Ga Liao, Shida Wang","doi":"10.1080/20002297.2025.2486650","DOIUrl":"https://doi.org/10.1080/20002297.2025.2486650","url":null,"abstract":"<p><strong>Background: </strong>Glycosylation is a ubiquitous biochemical process that covalently attaches glycans to proteins or lipids, which plays a pivotal role in modulating the structure and function of these biomolecules.  This post-translational modification is prevalent in living organisms and intricately regulates various biological processes, including signaling transduction, recognition, and immune responses.  In the oral environment, bacteria ingeniously use glycosylation to enhance their adhesion to oral surfaces, which is a key step in biofilm formation and subsequent development.  This adhesion process is intimately associated with the onset and progression of oral diseases, including dental caries and periodontal disease.</p><p><strong>Objective: </strong>This review aims to describe the types and mechanisms of glycosylation in oral bacteria, and to understand the role of glycosylation in the adhesion, biofilm formation and virulence of oral bacteria.</p><p><strong>Methods: </strong>We reviewed articles on glycosylation in a variety of oral bacteria.</p><p><strong>Conclusion: </strong>In cariogenic bacteria and periodontopathic pathogens, glycosylation facilitates adhesion and subsequent biofilm maturation on tooth surface.   Distinct glycosylation patterns in oral bacteria shape biofilm structure and function, influencing microbial interactions and community stability.   Pathogen-specific glycosylation signatures enhance virulence and ecological competitiveness, contributing to disease progression. Glycosylation plays a critical role in bacterial virulence and community  interactions, with significant implications for oral health and disease development.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2486650"},"PeriodicalIF":3.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11983588/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}