Journal of Oral Microbiology最新文献

{"title":"Oral microbiota and gastric cancer: recent highlights and knowledge gaps.","authors":"Ruihong Xia, Zhengchen Jiang, Ying Zhou, Libin Pan, Yanan Wang, Yubo Ma, Lili Fan, Li Yuan, Xiangdong Cheng","doi":"10.1080/20002297.2024.2391640","DOIUrl":"10.1080/20002297.2024.2391640","url":null,"abstract":"<p><p>Gastric cancer is one of the most common malignant tumors worldwide and has a high mortality rate. However, tests for the early screening and diagnosis of gastric cancer are limited and invasive. Certain oral microorganisms are over-expressed in gastric cancer, but there is heterogeneity among different studies. Notably, each oral ecological niche harbors specific microorganisms. Among them, tongue coating, saliva, and dental plaque are important and unique ecological niches in the oral cavity. The colonization environment in different oral niches may be a source of heterogeneity. In this paper, we systematically discuss the latest developments in the field of the oral microbiota and gastric cancer and elucidate the enrichment of microorganisms in the oral ecological niches of the tongue coatings, saliva, and dental plaque in gastric cancer patients. The various potential mechanisms by which the oral microbiota induces gastric cancer (activation of an excessive inflammatory response; promotion of proliferation, migration, invasion, and metastasis; and secretion of carcinogens, leading to imbalance in gastric microbial communities) are explored. In this paper, we also highlight the applications of the rapeutics targeting the oral microbiota in gastric cancer and suggests future research directions related to the relationship between the oral microbiota and gastric cancer.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"16 1","pages":"2391640"},"PeriodicalIF":3.7,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11332296/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142004439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted elimination of Fusobacterium nucleatum alleviates periodontitis","authors":"Nil Yakar, Ozge Unlu, Lujia Cen, Hatice Hasturk, Tsute Chen, Wenyuan Shi, Xuesong He, Alpdogan Kantarci","doi":"10.1080/20002297.2024.2388900","DOIUrl":"https://doi.org/10.1080/20002297.2024.2388900","url":null,"abstract":"Fusobacterium nucleatum, a pathobiont in periodontal disease, contributes to alveolar bone destruction. We assessed the efficacy of a new targeted antimicrobial, FP-100, in eradicating F. nucleatum...","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"47 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141945827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toll-like receptors 2 and 4, and bacterial proteins in IgG4-related sialadenitis, other types of chronic sialadenitis and sialolithiasis.","authors":"Elin Waltimo, Mine Eray, Antti Mäkitie, Caj Haglund, Timo Atula, Jaana Hagström","doi":"10.1080/20002297.2024.2382633","DOIUrl":"10.1080/20002297.2024.2382633","url":null,"abstract":"<p><strong>Background: </strong>The association of chronic sclerosing sialadenitis and IgG4-related disease (IgG4-RD) has resulted in the more frequent identification of IgG4-positivity in submandibular gland inflammations, also uncovering IgG4 overexpression in nonspecific inflammations. These findings lead us to hypothesise that IgG4-positive sialadenitis represents a continuous inflammatory process overlapping histologically with IgG4-RD, possibly differing in aetiology. However, the antigen underlying IgG4 overexpression in IgG4-positive sialadenitis and IgG4-RD remains unknown.</p><p><strong>Materials and methods: </strong>Here, we investigated toll-like receptor (TLR) - mediated bacterial inflammation in submandibular gland tissues of patients with IgG4-positive and IgG4-negative chronic inflammatory lesions of the submandibular gland (<i>n</i> = 61), with noninflamed submandibular glands serving as controls (<i>n</i> = 4). Utilising immunohistochemistry, we assessed the expression of TLR2 and TLR4, lipopolysaccharide (LPS) and the <i>P. gingivalis</i>-specific antigen gingipain R1.</p><p><strong>Results: </strong>We observed TLR2- and TLR4-immunopositivity in 64 (98%) samples. However, TLR2 and TLR4 staining intensity was significantly stronger in the IgG4-positive group. LPS- and gingipain R1 immunopositivity were observed in 56 (86%) and 58 (89%) samples, respectively. LPS-positivity localised exclusively in mast cell-like cells, while gingipain R1-positivity remained scarce.</p><p><strong>Conclusions: </strong>A stronger TLR2 or TLR4 expression in IgG4-positive sialadenitis may indicate a tissue-related factor underlying this form of chronic sialadenitis. LPS- and <i>P. gingivalis</i> immunopositivity remained weak throughout this series. Thus, gram-negative bacteria may not represent pathogens underlying these forms of chronic sialadenitis.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"16 1","pages":"2382633"},"PeriodicalIF":3.7,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11271128/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141759304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between oral microbial nitrate metabolism and poor prognosis in acute ischemic stroke patients with a history of hypertension.","authors":"Yueran Ren, Jingru Liang, Weike Hu, Jiahui Xie, Yifeng Zheng, Wei Song, Jiajia Zhu, Hongwei Zhou, Qiheng Wu, Yan He, Jia Yin","doi":"10.1080/20002297.2024.2382620","DOIUrl":"10.1080/20002297.2024.2382620","url":null,"abstract":"<p><strong>Background: </strong>Oral microbes mediate the production of nitric oxide (NO) through the denitrification pathway. This study aimed to investigate the association between oral microbial nitrate metabolism and prognosis in acute ischemic stroke (AIS) patients.</p><p><strong>Methods: </strong>This prospective, observational, single-center cohort study included 124 AIS patients admitted within 24 hours of symptom onset, with 24-hour ambulatory blood pressure data. Oral swabs were collected within 24 hours. Hypertensive AIS patients were stratified by the coefficient of variation (CV) of 24-hour systolic blood pressure. Microbial composition was analyzed using LEfSe and PICRUSt2 for bacterial and functional pathway identification.</p><p><strong>Results: </strong>Significant differences in oral microbiota composition were observed between hypertensive AIS patients with varying CVs. Lower CV groups showed enrichment of nitrate-reducing bacteria and \"Denitrification, nitrate => nitrogen\" pathways. The TAX score of oral nitrate-reducing bacteria, derived from LASSO modeling, independently correlated with 90-day modified Rankin Scale scores, serving as an independent risk factor for poor prognosis. Mediation analyses suggested indirect that the TAX score not only directly influences outcomes but also indirectly affects them by modulating 24-hour systolic blood pressure CV.</p><p><strong>Conclusions: </strong>AIS patients with comorbid hypertension and higher systolic blood pressure CV exhibited reduced oral nitrate-reducing bacteria, potentially worsening outcomes.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"16 1","pages":"2382620"},"PeriodicalIF":3.7,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11271073/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141759303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arginine-specific gingipains (RgpA/RgpB) knockdown modulates neutrophil machinery.","authors":"Vanessa Tubero Euzebio Alves, Tomaz Alves, Emanuel Silva Rovai, Hatice Hasturk, Thomas Van Dyke, Marinella Holzhausen, Alpdogan Kantarci","doi":"10.1080/20002297.2024.2376462","DOIUrl":"10.1080/20002297.2024.2376462","url":null,"abstract":"<p><strong>Background: </strong>Gingipains are important virulence factors present in Porphyromonas gingivalis. Arginine-specific gingipains (RgpA and RgpB) are critically associated with increased proteolytic activity and immune system dysfunction, including neutrophilic activity. In this study, we assessed the impact of gingipains (RgpA and RgpB) on neutrophil function.</p><p><strong>Methods: </strong>Peripheral blood samples were obtained; neutrophils were isolated and incubated with P. gingivalis A7436, W50, and the double RgpA/RgpB double knockout mutant E8 at MOI 20 for 2 hours. Neutrophil viability was assessed by Sytox staining. Phagocytic capacity and apoptosis were measured by flow cytometry. Superoxide release was measured by superoxide dismutase and cytochrome c reduction assay. Gene expression of TLR2, p47-phox, p67-phox, and P2 × 7was measured by qPCR. Inflammatory cytokine and chemokine production was measured by IL-1β, IL-8, RANTES, and TNF-α in cell supernatants.</p><p><strong>Results: </strong>Neutrophil TLR2 gene expression was reduced in the absence of RgpA/RgpB (<i>p</i> < 0.05), while superoxide production was not significantly impacted. RgpA/RgpB^-/- significantly impaired neutrophil phagocytic function (<i>p</i> < 0.05) and increased TNF-α production when compared with the wild-type control (<i>p</i> < 0.05). Neutrophil apoptosis was not altered when exposed to RgpA/RgpB^-/- E8 (<i>p</i> > 0.05).</p><p><strong>Conclusion: </strong>These data suggest that arginine-specific gingipains (RgpA/RgpB) can modulate neutrophil responses against <i>P. gingivalis</i> infection.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"16 1","pages":"2376462"},"PeriodicalIF":3.7,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11234918/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141580014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dysbiosis and interactions of the mycobiome and bacteriome in mucosal lesions of erosive and non-erosive oral lichen planus patients.","authors":"Liang Beibei, Wei Mengying, Huo Xiao, Jing Yuzi, Mi Lijin, Zhang Ke, Yi Shengjie, Liu Li","doi":"10.1080/20002297.2024.2374639","DOIUrl":"10.1080/20002297.2024.2374639","url":null,"abstract":"<p><strong>Background: </strong>Oral lichen planus (OLP) is a common oral mucosal disease, clinically categorized into erosive OLP (EOLP) and non-erosive OLP (NEOLP) based on symptoms, but its pathogenic mechanism remains unclear. This study aims to explore the relationship between OLP and the oral microbiome.</p><p><strong>Methods: </strong>We collected oral mucosal samples from 49 patients and 10 healthy individuals and conducted 16S rRNA and ITS gene sequencing to explore the oral fungal and bacterial communities.</p><p><strong>Results: </strong>We observed significantly lower α diversity of fungi in the EOLP group, with Candida being significantly enriched as the main dominant genus. In the NEOLP group, Aspergillaceae were significantly enriched. The EOLP group showed significant enrichment of Aggregatibacter and Lactobacillus, but the relative abundance of Streptococcus was notably lower than in the other two groups. In the NEOLP group, two species including Prevotella intermedia were significantly enriched. The microbial co-occurrence and co-exclusion networks display distinct characteristics across the three groups, with Lactobacillus assuming a significant bridging role in the ELOP group.</p><p><strong>Conclusions: </strong>Our study indicates that EOLP and NEOLP experience varying degrees of dysbiosis at both the fungal and bacterial levels. Therefore, the pathogenic mechanisms and interactive relationships of these microbiota associated with OLP merit further in-depth investigation.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"16 1","pages":"2374639"},"PeriodicalIF":3.7,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11229720/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141558975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supragingival microbiota, cytokines, and proteins in individuals with different trajectories in experimental gingivitis.","authors":"Christine Lundtorp-Olsen, Nikoline Nygaard, Laura Massarenti, Florentin Constancias, Christian Damgaard, Ulvi Kahraman Gursoy, Annina van Splunter, Floris J Bikker, Mervi Gursoy, Merete Markvart, Daniel Belstrøm","doi":"10.1080/20002297.2024.2372861","DOIUrl":"10.1080/20002297.2024.2372861","url":null,"abstract":"<p><strong>Background: </strong>Gingivitis in response to biofilm formation may exhibit different trajectories. The purposes of the present study were to characterize the composition of the supragingival microbiota and salivary cytokine and protein levels in healthy individuals with different gingivitis patterns, to test the hypothesis that manifestations of gingivitis associate with specific profiles in terms of supragingival microbiota, salivary cytokines, and proteins.</p><p><strong>Methods: </strong>Forty orally and systemically healthy individuals refrained from all oral hygiene procedures for a period of 14 days, followed by a resolution period of 14 days with regular oral care. Supragingival plaque level and bleeding on probing (BOP) were recorded, and supragingival plaque as well as saliva samples were collected at baseline, day 14, and day 28. Based on change in BOP% from baseline to day 14, rapid (<i>n</i> = 15), moderate (<i>n</i> = 10), and slow (<i>n</i> = 15) responders were identified. Supragingival microbiota composition, salivary cytokine, and protein levels were compared between groups at baseline, day 14, and day 28.</p><p><strong>Results: </strong>A significantly higher baseline abundance of <i>Capnocytophaga, Eikenella</i>, and <i>Campylobacter</i> species were recorded in rapid responders, whereas a significantly higher baseline abundance of <i>Streptococcus</i> species were detected in slow responders. Slow responders expressed a high degree of resilience, with minimal difference in microbial composition at baseline and after 14 days of resolution (day 28). On the contrary, significant differences in relative abundance of members of the core microbiota, <i>Streptococcus</i>, <i>Actinomyces</i>, and <i>Rothia</i> species, was noted in baseline samples versus day 28 samples in rapid responders. Comparable baseline cytokine and protein levels were recorded in all groups.</p><p><strong>Conclusion: </strong>Supragingival microbiota composition, but not saliva cytokine and protein profiles, seems to influence the extent of the inflammatory response during development of gingivitis in systemically healthy individuals.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"16 1","pages":"2372861"},"PeriodicalIF":3.7,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11229773/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141558976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin inhibits growth of <i>Porphyromonas gingivalis</i> by arrest of bacterial dipeptidyl peptidase activity.","authors":"Hiroki Murai, Masae Kuboniwa, Miho Kakiuchi, Reiko Matsumura, Yoshihiko Hirata, Atsuo Amano","doi":"10.1080/20002297.2024.2373040","DOIUrl":"10.1080/20002297.2024.2373040","url":null,"abstract":"<p><strong>Background: </strong>Curcumin is a multi-functional polyphenol with anti-bacterial and anti-inflammatory effects and may have potential for treatment of periodontal diseases. The present study was conducted to examine the molecular basis of the anti-bacterial effect of curcumin against <i>Porphyromonas gingivalis</i> using metabolome analysis.</p><p><strong>Materials and methods: </strong><i>P. gingivalis</i> were incubated with 10 µg/mL curcumin, and then metabolites were analyzed with CE-TOF/MS. Expression levels of sigma factors were also evaluated using RT-PCR assays. The activities of dipeptidyl peptidases (DPPs) were assessed by examining the degradation reactions of MCA-labeled peptides.</p><p><strong>Results: </strong>The relative amounts of various glycogenic amino acids were significantly decreased when <i>P. gingivalis</i> was incubated with curcumin. Furthermore, the metabolites on the amino acid degradation pathway, including high-energy compounds such as ATP, various intermediate metabolites of RNA/DNA synthesis, nucleoside sugars and amino sugars were also decreased. Additionally, the expression levels of sigma-54 and sigma-70 were significantly decreased, and the same results as noted following nutrient starvation. Curcumin also significantly suppressed the activities of some DPPs, while the human DPP-4 inhibitors markedly inhibited the growth of <i>P. gingivalis</i> and activities of the DPPs.</p><p><strong>Conclusions: </strong>Curcumin suppresses the growth of <i>P. gingivalis</i> by inhibiting DPPs and also interferes with nucleic acid synthesis and central metabolic pathways, beginning with amino acid metabolism.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"16 1","pages":"2373040"},"PeriodicalIF":3.7,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11225630/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141554935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of mobile app-based oral hygiene instructions on clinical parameters, oral bacterial diversity, and composition of subgingival microbiota in periodontitis patients.","authors":"Melinda Rabekka Purba, Mardikacandra Manggala Putra, Benso Sulijaya, Adityo Widaryono, Valdy Hartono, Yoga Setiadharma, Aurelle Khadeeja Rizany, Fatimah Maria Tadjoedin, Marie Rossini Carmela T Lachica","doi":"10.1080/20002297.2024.2372206","DOIUrl":"10.1080/20002297.2024.2372206","url":null,"abstract":"<p><strong>Introduction: </strong>Oral hygiene instruction (OHI) is essential during periodontitis treatment. Various OHI approaches have been explored, including mobile apps.</p><p><strong>Objective: </strong>To evaluate the mobile app-based OHI's effect on periodontitis management by analyzing clinical parameters and subgingival microbiota.</p><p><strong>Methods: </strong>Forty-four periodontitis patients were randomly assigned into two groups. The test group (<i>n</i> = 22) received scaling and root planing (SRP), OHI, and mobile app-based OHI, whereas the control group (<i>n</i> = 22) received SRP and OHI. Full mouth plaque score (FMPS), bleeding on probing (BOP) and probing pocket depth at the sampling sites (site-PPD) were assessed at baseline, one- and three-month visits. The 16S rRNA next-generation sequencing (NGS) was used to analyze subgingival plaque samples.</p><p><strong>Results: </strong>Significant reduction in FMPS, BOP, and site-PPD at one- and three-month visits compared to baseline (<i>p</i> < 0.001) with no significant differences across groups (<i>p</i> > 0.05). In test groups, intra-group analysis showed better improvement in BOP and site-PPD (<i>p</i> < 0.05) than control. The diversity and composition of subgingival microbiota did not differ between groups or timepoints (<i>p</i> > 0.05).</p><p><strong>Conclusions: </strong>Mobile app-based OHI showed no superior effects on improving clinical parameters and subgingival microbiota compared to conventional OHI. Further investigation into its long-term impact on periodontitis treatment is needed.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"16 1","pages":"2372206"},"PeriodicalIF":3.7,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11212576/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141468795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of sodium lauryl sulfate and postbiotic toothpaste on oral microecology.","authors":"Qingying Shi, Lianlian Sun, Jing Gao, Fengzhu Li, Dongxiao Chen, Tingting Shi, Youlan Tan, Huimin Chang, Xiaozhi Liu, Jian Kang, Fuping Lu, Zhengmei Huang, Huabing Zhao","doi":"10.1080/20002297.2024.2372224","DOIUrl":"https://doi.org/10.1080/20002297.2024.2372224","url":null,"abstract":"<p><p>The diversity and delicate balance of the oral microbiome contribute to oral health, with its disruption leading to oral and systemic diseases. Toothpaste includes elements like traditional additives such as sodium lauryl sulfate (SLS) as well as novel postbiotics derived from probiotics, which are commonly employed for maintaining oral hygiene and a healthy oral cavity. However, the response of the oral microbiota to these treatments remains poorly understood. In this study, we systematically investigated the impact of SLS, and toothpaste containing postbiotics (hereafter, postbiotic toothpaste) across three systems: biofilms, animal models, and clinical populations. SLS was found to kill bacteria in both preformed biofilms (mature biofilms) and developing biofilms (immature biofilms), and disturbed the microbial community structure by increasing the number of pathogenic bacteria. SLS also destroyed periodontal tissue, promoted alveolar bone resorption, and enhanced the extent of inflammatory response level. The postbiotic toothpaste favored bacterial homeostasis and the normal development of the two types of biofilms <i>in vitro</i>, and attenuated periodontitis and gingivitis <i>in vivo</i> via modulation of oral microecology. Importantly, the postbiotic toothpaste mitigated the adverse effects of SLS when used in combination, both <i>in vitro</i> and <i>in vivo</i>. Overall, the findings of this study describe the impact of toothpaste components on oral microflora and stress the necessity for obtaining a comprehensive understanding of oral microbial ecology by considering multiple aspects.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"16 1","pages":"2372224"},"PeriodicalIF":3.7,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11210412/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141468796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}