Journal of Oral Microbiology最新文献_第2页

The association between the oral microbiome and hypertension: a systematic review.

IF 3.7 2区医学

Journal of Oral Microbiology Pub Date : 2025-02-02 eCollection Date: 2025-01-01 DOI: 10.1080/20002297.2025.2459919

Sadeq Ali Al-Maweri, Abeer A Al-Mashraqi, Gamilah Al-Qadhi, Nezar Al-Hebshi, Raidan Ba-Hattab

{"title":"The association between the oral microbiome and hypertension: a systematic review.","authors":"Sadeq Ali Al-Maweri, Abeer A Al-Mashraqi, Gamilah Al-Qadhi, Nezar Al-Hebshi, Raidan Ba-Hattab","doi":"10.1080/20002297.2025.2459919","DOIUrl":"10.1080/20002297.2025.2459919","url":null,"abstract":"Background: This study systematically reviewed the available evidence regarding the potential association between oral microbiota and hypertension.Methods: A comprehensive search of online databases was conducted by two independent investigators for all relevant articles. All observational studies that assessed the association between oral microbiota and hypertension were included. Quality appraisal was conducted using the NOS tool.Results: A total of 17 studies comprising 6007 subjects were included. The studies varied with respect to sample type and microbial analysis method. All studies, except one, found significant differences in microbial composition between hypertensive and normotensive subjects. However, there were substantial inconsistencies regarding the specific differences identified. Still, a few taxa were repeatedly found enriched in hypertension including Aggregatibacter, Kingella, Lautropia, and Leptotrachia besides the red complex periodontal pathogens. When considering only studies that controlled for false discovery rates and confounders, Atopobium, Prevotella, and Veillonella were identified as consistently associated with hypertension.Conclusion: There are significant differences in the oral microbiome between hypertensive and normotensive subjects. Despite the heterogeneity between the included studies, a subset of microbial taxa seems to be consistently enriched in hypertension. Further studies are highly recommended to explore this association.Registration: PROSPERO database (ID: CRD42023495005).","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2459919"},"PeriodicalIF":3.7,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11789219/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143123077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparison of the alterations of oral microbiome with fixed orthodontic therapy and clear aligners: a systematic review.

IF 3.7 2区医学

Journal of Oral Microbiology Pub Date : 2025-02-01 eCollection Date: 2025-01-01 DOI: 10.1080/20002297.2024.2372751

Alessandra Lucchese, Marta Marcolina, Nicasio Mancini, Roberto Ferrarese, Serena Acconciaioco, Enrico Gherlone, Chiara Bonini, Maurizio Manuelli, Antonella Polimeni

{"title":"A comparison of the alterations of oral microbiome with fixed orthodontic therapy and clear aligners: a systematic review.","authors":"Alessandra Lucchese, Marta Marcolina, Nicasio Mancini, Roberto Ferrarese, Serena Acconciaioco, Enrico Gherlone, Chiara Bonini, Maurizio Manuelli, Antonella Polimeni","doi":"10.1080/20002297.2024.2372751","DOIUrl":"10.1080/20002297.2024.2372751","url":null,"abstract":"Aim: The oral microbiome plays a fundamental role in maintaining homeostasis of the oral cavity. In the last decade there has been an increasing use of clear aligners, which guarantee aesthetics and comfort for the patient. The aim of this work is to conduct a systematic review regarding the alterations in bacterial flora and oral health with aligner and fixed orthodontic therapy.Design: A systematic review was conducted following the PRISMA Statement. Using the search strategy \"(clear aligners OR Invisalign) AND (fixed therapy OR fixed orthodont * therapy) NOT (thermoplastic retainers) AND (oral microbiome OR oral microbiota * OR oral microbiology * OR oral health)\", in the main scientific databases. Two scales were applied to assess the quality of scientific evidence: ROBINS-I and RoB 2.Results: A total of 484 articles emerged of which 9 met our inclusion/exclusion criteria. Afterwards the application of the rating scales, 1 article was found to be at low risk of bias, 6 at moderate and 2 at serious risk of bias.Conclusion: Both therapies cause an alteration of the oral microbiome, but the changes induced by the aligners seem to be compatible with a better oral health compared to fixed appliances.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2372751"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11789220/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143123019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bidirectional effects of neutrophils on Streptococcus oralis biofilms in vitro.

IF 3.7 2区医学

Journal of Oral Microbiology Pub Date : 2025-01-23 eCollection Date: 2025-01-01 DOI: 10.1080/20002297.2025.2453986

Basmah M Almaarik, Rizwan Ali, Paul R Cooper, Michael R Milward, Josefine Hirschfeld

{"title":"Bidirectional effects of neutrophils on Streptococcus oralis biofilms in vitro.","authors":"Basmah M Almaarik, Rizwan Ali, Paul R Cooper, Michael R Milward, Josefine Hirschfeld","doi":"10.1080/20002297.2025.2453986","DOIUrl":"10.1080/20002297.2025.2453986","url":null,"abstract":"Background: Streptococcus oralis is a commensal bacterium and an early biofilm coloniser found in the human oral cavity. One of the biofilm matrix constituents is bacterial extracellular DNA (eDNA). Neutrophils are innate immune cells that respond to biofilms, employing antimicrobial mechanisms such as neutrophil extracellular trap (NET) and reactive oxygen species (ROS) release. Here, bidirectional effects of neutrophils on S. oralis biofilms were investigated.Materials and methods: Isolated neutrophils were introduced to S. oralis biofilms at different stages of biofilm development. Biofilm quantity was assessed by crystal violet technique, confocal microscopy and CFU enumeration. Surface adhesion during shear stress was quantified by spectrophotometry. Bacterial and neutrophil extracellular DNA within biofilms and ROS production were analysed using fluorescence and luminescence assays, and neutrophil-eDNA interactions were investigated by flow cytometry and fluorescence microscopy.Results: Neutrophils decreased S. oralis biofilm quantity transiently and reduced eDNA but did not affect biofilm surface adhesion. Unexpectedly, CFUs were increased by neutrophils. Bacterial DNA was found to co-localise with neutrophil membranes. Neutrophils produced elevated total and intracellular ROS, however, no NETs in response to biofilms.Conclusion: Neutrophils in vitro are not excessively activated by S. oralis biofilms but are able to reduce biofilm quantity in the short-term, possibly through interfering with eDNA.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2453986"},"PeriodicalIF":3.7,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11758797/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral Fusobacterium nucleatum resists the acidic pH of the stomach due to membrane erucic acid synthesized via enoyl-CoA hydratase-related protein FnFabM.

IF 3.7 2区医学

Journal of Oral Microbiology Pub Date : 2025-01-21 eCollection Date: 2025-01-01 DOI: 10.1080/20002297.2025.2453964

Xiaocong Li, Shipeng Zhang, Huafang Sheng, Yan Zhen, Buling Wu, Zhuang Li, Dingqiang Chen, Hongwei Zhou

{"title":"Oral Fusobacterium nucleatum resists the acidic pH of the stomach due to membrane erucic acid synthesized via enoyl-CoA hydratase-related protein FnFabM.","authors":"Xiaocong Li, Shipeng Zhang, Huafang Sheng, Yan Zhen, Buling Wu, Zhuang Li, Dingqiang Chen, Hongwei Zhou","doi":"10.1080/20002297.2025.2453964","DOIUrl":"10.1080/20002297.2025.2453964","url":null,"abstract":"Background and objective: Oral bacteria can translocate to the intestine, and their colonization efficiency is influenced by the gastrointestinal tract pH. Understanding how oral bacteria resist acidic environments is crucial for elucidating their role in gut health and disease.Methods: To investigate the mechanisms of acid resistance in oral bacteria, an in vitro gastrointestinal tract Dynamic pH Model was established. This model was used to simulate the acidic conditions encountered by bacteria during their translocation from the mouth to the intestine.Results: Fusobacterium nucleatum exhibited the highest survival rate in an acidified fluid mimicking the stomach pH (pH 1.5). The survival was significantly increased in the presence of erucic acid C22:1(n9) in cell membranes. Phylogenetic tree analysis revealed that C22:1(n9) synthesis was significantly associated with FnFabM gene expression in F. nucleatum at pH 1.5. Inhibition of FnFabM expression by cerulenin reduced the C22:1(n9) content and decreased the colonization efficiency of F. nucleatum in the stomach and jejunum of mice.Conclusions: Oral F. nucleatum translocate to the intestine by resisting the acidic environment owing to the presence of erucic acid in its cell membrane, which is regulated by FnFabM. These results provide novel insights into the mechanisms underlying the oral bacteria survival in acidic environments and their potential to colonize the intestine; thus, shedding light on the oral-gut axis and its implications on human health.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2453964"},"PeriodicalIF":3.7,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11753016/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143023724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An oral microbiota-based deep neural network model for risk stratification and prognosis prediction in gastric cancer. 基于口腔微生物群的胃癌风险分层及预后预测的深度神经网络模型。

IF 3.7 2区医学

Journal of Oral Microbiology Pub Date : 2025-01-17 eCollection Date: 2025-01-01 DOI: 10.1080/20002297.2025.2451921

Xue-Feng Gao, Can-Gui Zhang, Kun Huang, Xiao-Lin Zhao, Ying-Qiao Liu, Zi-Kai Wang, Rong-Rong Ren, Geng-Hui Mai, Ke-Ren Yang, Ye Chen

{"title":"An oral microbiota-based deep neural network model for risk stratification and prognosis prediction in gastric cancer.","authors":"Xue-Feng Gao, Can-Gui Zhang, Kun Huang, Xiao-Lin Zhao, Ying-Qiao Liu, Zi-Kai Wang, Rong-Rong Ren, Geng-Hui Mai, Ke-Ren Yang, Ye Chen","doi":"10.1080/20002297.2025.2451921","DOIUrl":"10.1080/20002297.2025.2451921","url":null,"abstract":"Background: This study aims to develop an oral microbiota-based model for gastric cancer (GC) risk stratification and prognosis prediction.Methods: Oral microbial markers for GC prognosis and risk stratification were identified from 99 GC patients, and their predictive potential was validated on an external dataset of 111 GC patients. The identified bacterial markers were used to construct a Deep Neural Network (DNN) model, a Random Forest (RF) model, and a Support Vector Machine (SVM) model for predicting GC prognosis.Results: GC patients with <3 years of survival showed a higher abundance of Aggregatibacter and diminished abundances of Filifactor and Moryella than those who survived ≥3 years. The Boruta algorithm unearthed Leptotrichia as another significant marker for GC prognosis. Consequently, a DNN model was constructed based on the relative abundances of these bacteria, predicting 3-year and 5-year survival in GC patients with Area Under Curve of 0.814 and 0.912, respectively. Notably, the DNN model outperformed the TNM staging system, SVM and RF models. The prognostic value of these bacterial markers was further reinforced by external validation.Conclusion: The oral microbiota-based DNN model may advance GC prognosis. The biological functions of these oral bacterial markers warrant further investigation from the perspective of GC progression.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2451921"},"PeriodicalIF":3.7,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11749243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143007020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Platelet-rich fibrin obtained from different protocols affects the formation of the in vitro multispecies subgingival biofilm associated with periodontitis. 从不同方案获得的富血小板纤维蛋白影响与牙周炎相关的体外多物种龈下生物膜的形成。

IF 3.7 2区医学

Journal of Oral Microbiology Pub Date : 2025-01-01 DOI: 10.1080/20002297.2024.2445598

Fabio Hideaki Uyeda, Gustavo Quilles Vargas, Larissa Matias Malavazi, Tatiane Tiemi Macedo, Aline Paim de Abreu Paulo Gomes, Manuela Rocha Bueno, Pedro Henrique Moreira Paulo Tolentino, Lucas Daylor Aguiar da Silva, Luciene Cristina Figueiredo, Jamil Awad Shibli, Bruno Bueno-Silva

{"title":"Platelet-rich fibrin obtained from different protocols affects the formation of the in vitro multispecies subgingival biofilm associated with periodontitis.","authors":"Fabio Hideaki Uyeda, Gustavo Quilles Vargas, Larissa Matias Malavazi, Tatiane Tiemi Macedo, Aline Paim de Abreu Paulo Gomes, Manuela Rocha Bueno, Pedro Henrique Moreira Paulo Tolentino, Lucas Daylor Aguiar da Silva, Luciene Cristina Figueiredo, Jamil Awad Shibli, Bruno Bueno-Silva","doi":"10.1080/20002297.2024.2445598","DOIUrl":"10.1080/20002297.2024.2445598","url":null,"abstract":"Background: The aim of this article is to evaluate the effect of different portions of Platelet Rich Fibrin (PRF) membranes and liquid-PRF, prepared by two distinct protocols/centrifuges each, on the multispecies subgingival biofilm.Materials and methods: PRF membranes and liquid-PRF were prepared using two protocols: centrifuge 1 uses fixed acceleration while centrifuge 2, progressive acceleration. PRF samples were introduced into device concurrently with 33-species bacterial inoculum. After seven days, biofilm metabolic activity (MA) and microbial profile were evaluated through colorimetric reaction and DNA-DNA hybridization, respectively.Results: Among PRF membranes, the ones from centrifuge 1 led to better reduction in MA, total biofilm, and F. periodonticum, P. gingivalis and T. forsythia counts when compared to untreated/centrifuge 2 treated biofilms. However, centrifuge 2 liquid-PRF reduced MA, total biofilm and F. periodonticum counts when compared to untreated/centrifuge 1 treated-biofilms.Conclusion: PRF membrane and exhibited comparable antibiofilm activity. However, PRF distinct forms, obtained by same centrifugation protocol, may present different antimicrobial properties.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2445598"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11722119/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The comparative effect of propolis and chlorhexidine mouthwash on oral nitrite-producing bacteria and blood pressure regulation. 蜂胶漱口水与氯己定漱口水对口腔亚硝酸盐产生菌及血压调节的比较研究。

IF 3.7 2区医学

Journal of Oral Microbiology Pub Date : 2024-12-16 eCollection Date: 2025-01-01 DOI: 10.1080/20002297.2024.2439636

R Bescos, L du Toit, A Redondo-Rio, P J Warburton, T L Nicholas, M Kiernan, R A Burton, L Belfield, G Montagut, A Benavente, W Vevers, T Gabaldón, Z Brookes, P Casas-Agustench

{"title":"The comparative effect of propolis and chlorhexidine mouthwash on oral nitrite-producing bacteria and blood pressure regulation.","authors":"R Bescos, L du Toit, A Redondo-Rio, P J Warburton, T L Nicholas, M Kiernan, R A Burton, L Belfield, G Montagut, A Benavente, W Vevers, T Gabaldón, Z Brookes, P Casas-Agustench","doi":"10.1080/20002297.2024.2439636","DOIUrl":"10.1080/20002297.2024.2439636","url":null,"abstract":"Background: Propolis mouthwash (PROP-M) has demonstrated antibacterial properties like those of chlorhexidine mouthwash (CHX-M). However, its impact on the abundance of oral nitrite-producing species (NPS) and nitrite-producing activity (NPA) remains unexplored.Methods: Forty-five healthy individuals were randomised into 2 groups to rinse their mouth twice a day for seven days with either CHX-M (n = 21) or PROP-M (n = 24). Metagenomic sequencing (16S rRNA) was performed on saliva samples collected before and after each treatment. Additionally, salivary biomarkers and blood pressure were measured.Results: CHX-M increased the relative abundance of NPS (p < 0.001) but significantly impaired the NPA (p < 0.001) compared to baseline and PROP-M. No significant differences in the relative abundance of NPS and NPA were observed in the PROP-M group. However, a significant increase of plasma nitrate (+7 µmol/L, p = 0.047) and a decrease in systolic BP (-2 mmHg, p = 0.022) was observed in this group compared to the baseline.Conclusion: The results indicate that PROP-M had a smaller effect on the abundance of NPS and NPA compared to CHX-M. Additionally, PROP-M reduced blood pressure in healthy individuals, but this effect was not associated with changes in the oral microbiome.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2439636"},"PeriodicalIF":3.7,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11650436/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142846766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral administration of Porphyromonas gingivalis to mice with diet-induced obesity impairs cognitive function associated with microglial activation in the brain. 饮食诱发肥胖的小鼠口服牙龈卟啉单胞菌会损害与大脑小胶质细胞活化相关的认知功能。

IF 3.7 2区医学

Journal of Oral Microbiology Pub Date : 2024-11-14 eCollection Date: 2024-01-01 DOI: 10.1080/20002297.2024.2419155

Kana Oue, Yosuke Yamawaki, Kazuhisa Ouhara, Eiji Imado, Tetsuya Tamura, Mitsuru Doi, Yoshitaka Shimizu, Mitsuhiro Yoshida, Noriyoshi Mizuno, Norimitsu Morioka, Takashi Kanematsu, Masahiro Irifune, Yukio Ago

{"title":"Oral administration of Porphyromonas gingivalis to mice with diet-induced obesity impairs cognitive function associated with microglial activation in the brain.","authors":"Kana Oue, Yosuke Yamawaki, Kazuhisa Ouhara, Eiji Imado, Tetsuya Tamura, Mitsuru Doi, Yoshitaka Shimizu, Mitsuhiro Yoshida, Noriyoshi Mizuno, Norimitsu Morioka, Takashi Kanematsu, Masahiro Irifune, Yukio Ago","doi":"10.1080/20002297.2024.2419155","DOIUrl":"10.1080/20002297.2024.2419155","url":null,"abstract":"Objective: Both periodontal disease and obesity are risk factors for dementia, but their links to 1brain function remain unclear. In this study, we examined the effects of oral infection with a periodontal pathogen on cognitive function in a mouse model of obesity, focusing on the roles of microglia.Methods: To create a mouse model of diet-induced obesity and periodontitis, male C57BL/6 J mice were first fed a high-fat diet containing 60% lipid calories for 18 weeks, beginning at 12 weeks of age, to achieve diet-induced obesity. Then, Porphyromonas gingivalis administration in the oral cavity twice weekly for 6 weeks was performed to induce periodontitis in obese mice.Results: Obese mice orally exposed to P. gingivalis showed cognitive impairment in the novel object recognition test. Increased expression levels of inflammatory cytokines (e.g. interleukin-1β and tumor necrosis factor-α) were observed in the hippocampus of P. gingivalis-treated obese mice. Immunohistochemical analysis revealed that microglia cell body size was increased in the hippocampus and prefrontal cortex of P. gingivalis-treated obese mice, indicating microglial activation. Furthermore, depletion of microglia by PLX3397, a colony-stimulating factor 1 receptor inhibitor, ameliorated cognitive dysfunction.Conclusion: These results suggest that microglia mediate periodontal infection-induced cognitive dysfunction in obesity.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"16 1","pages":"2419155"},"PeriodicalIF":3.7,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11565673/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142647359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RE: colonization of bacterial and viral respiratory pathogens among healthcare workers in China during COVID-19 pandemic. RE: COVID-19 大流行期间中国医护人员呼吸道病原体的细菌和病毒定植。

IF 3.7 2区医学

Journal of Oral Microbiology Pub Date : 2024-11-11 eCollection Date: 2024-01-01 DOI: 10.1080/20002297.2024.2427698

Hineptch Daungsupawong, Viroj Wiwanitkit

引用次数: 0

In vitro plaque formation model to unravel biofilm formation dynamics on implant abutment surfaces. 体外菌斑形成模型，揭示种植体基台表面生物膜形成动力学。

IF 3.7 2区医学

Journal of Oral Microbiology Pub Date : 2024-11-08 eCollection Date: 2024-01-01 DOI: 10.1080/20002297.2024.2424227

Romualdus Nugraha Catur Utomo, Alena Lisa Palkowitz, Lin Gan, Anna Rudzinski, Julia Franzen, Hendrik Ballerstedt, Martin Zimmermann, Lars Mathias Blank, Horst Fischer, Stefan Wolfart, Taskin Tuna

{"title":"In vitro plaque formation model to unravel biofilm formation dynamics on implant abutment surfaces.","authors":"Romualdus Nugraha Catur Utomo, Alena Lisa Palkowitz, Lin Gan, Anna Rudzinski, Julia Franzen, Hendrik Ballerstedt, Martin Zimmermann, Lars Mathias Blank, Horst Fischer, Stefan Wolfart, Taskin Tuna","doi":"10.1080/20002297.2024.2424227","DOIUrl":"https://doi.org/10.1080/20002297.2024.2424227","url":null,"abstract":"Background: Biofilm formation on implant-abutment surfaces can cause inflammatory reactions. Ethical concerns often limit intraoral testing, necessitating preliminary in vitro or animal studies. Here, we propose an in vitro model using human saliva and hypothesize that this model has the potential to closely mimic the dynamics of biofilm formation on implant-abutment material surfaces in vivo.Methods: A saliva stock was mixed with modified Brain-Heart-Infusion medium to form biofilms on Titanium-Aluminum-Vanadium (Ti6Al4V) and Yttria-partially Stabilized Zirconia (Y-TZP) discs in 24-well plates. Biofilm analyses included crystal violet staining, intact cell quantification with BactoBox, 16S rRNA gene analysis, and short-chain fatty acids measurement. As a control, discs were worn in maxillary splints by four subjects for four days to induce in vivo biofilm formation.Results: After four days, biofilms fully covered Ti6Al4V and Y-TZP discs both in vivo and in vitro, with similar cell viability. There was a 60.31% overlap of genera between in vitro and in vivo biofilms in the early stages, and 41% in the late stages. Ten key oral bacteria, including Streptococcus, Haemophilus, Neisseria, Veillonella, and Porphyromonas, were still detectable in vitro, representing the common stages of oral biofilm formation.Conclusion: This in vitro model effectively simulates oral conditions and provides valuable insights into biofilm dynamics.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"16 1","pages":"2424227"},"PeriodicalIF":3.7,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11552293/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142622283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0