PI3K/AKT/mTOR通路在口腔鳞状细胞癌复发中的作用

IF 5.5 2区 医学 Q2 MICROBIOLOGY
Journal of Oral Microbiology Pub Date : 2025-09-20 eCollection Date: 2025-01-01 DOI:10.1080/20002297.2025.2560020
Da-Woon Kwack, Zeba Praveen, Yeon-Hee Kim, Chong Woo Yoo, Jae Hee Ko, Suk Min Youn, Joo Yong Park, Jong-Ho Lee, Sung Weon Choi, Mi Kyung Kim
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引用次数: 0

摘要

背景:口腔鳞状细胞癌(OSCC)经常局部复发,降低生存率。口腔微生物组可能影响肿瘤复发,但其预后作用尚不清楚。本研究探讨口腔微生物群与OSCC复发的关系及其预后价值。材料与方法:收集133例OSCC患者的唾液样本。进行16S rRNA基因测序,并通过XGBoost预测微生物特征。使用PICRUSt2进行功能宏基因组预测。结果:XGBoost鉴定出真菌菌、乳杆菌、金氏菌、Paludibacter、Parvimonas、葡萄球菌和Veillonella可预测OSCC复发。真杆菌和乳酸杆菌在复发性疾病中显著富集,并与较差的生存率相关。葡萄球菌和细孔菌在非复发性疾病中大量存在,与良好的预后相关。与临床n期模型(AUC = 0.66)相比,基于微生物组的模型获得了更好的预测性能(AUC = 0.741)。真菌菌和乳酸菌与磷酸肌肽3激酶(PI3K)/AKT/哺乳动物雷帕霉素靶蛋白(mTOR)通路中蛋白激酶B (AKT)、成纤维细胞生长因子受体1和鸟嘌呤核苷酸结合蛋白G亚基β -2等关键基因呈正相关。相比之下,细孔菌与这些基因呈负相关。结论:口腔唾液微生物组分析揭示了与OSCC复发相关的独特微生物模式。我们基于相关性的功能预测表明,真菌菌和乳酸菌的富集以及微孔菌的低丰度可能通过致癌PI3K/AKT/mTOR影响复发,强调了基于唾液的微生物生物标志物的预后潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Oral microbial signatures underlying recurrence via PI3K/AKT/mTOR pathway modulation in oral squamous cell carcinoma.

Background: Oral squamous cell carcinoma (OSCC) often recurs locally, reducing survival. The oral microbiome may influence tumor recurrence, but its prognostic role is unclear. This study investigated oral microbiomes associated with OSCC recurrence and their prognostic merit.

Materials and methods: Saliva samples were collected from 133 patients with OSCC. 16S rRNA gene sequencing was performed, and microbial signatures were predicted via XGBoost. Functional metagenomic prediction was conducted using PICRUSt2.

Results: XGBoost identified Eubacterium, Lactobacillus, Kingella, Paludibacter, Parvimonas, Staphylococcus, and Veillonella as predictive for OSCC recurrence. Eubacterium and Lactobacillus were significantly enriched in recurrent disease and associated with poor survival. Staphylococcus and Veillonella were abundant in non-recurrent disease, correlating with a favorable prognosis. The microbiome-based model achieved superior predictive performance (AUC = 0.741) compared with the clinical N-stage model (AUC = 0.66). Eubacterium and Lactobacillus showed positive correlations with key genes, such as protein kinase B (AKT), fibroblast growth factor receptor 1 and guanine nucleotide-binding protein G subunit beta-2, within the phosphoinositide 3-kinase (PI3K)/AKT/mammalian target of rapamycin (mTOR) pathway. In contrast, Veillonella was negatively correlated with these genes.

Conclusions: Oral saliva microbiome profiling reveals distinct microbial patterns associated with OSCC recurrence. Our correlation-based functional predictions indicated that the enrichment of Eubacterium and Lactobacillus along with a lower abundance of Veillonella may influence recurrence through oncogenic PI3K/AKT/mTOR, underscoring the prognostic potential of saliva-based microbial biomarkers.

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来源期刊
CiteScore
8.00
自引率
4.40%
发文量
52
审稿时长
12 weeks
期刊介绍: As the first Open Access journal in its field, the Journal of Oral Microbiology aims to be an influential source of knowledge on the aetiological agents behind oral infectious diseases. The journal is an international forum for original research on all aspects of ''oral health''. Articles which seek to understand ''oral health'' through exploration of the pathogenesis, virulence, host-parasite interactions, and immunology of oral infections are of particular interest. However, the journal also welcomes work that addresses the global agenda of oral infectious diseases and articles that present new strategies for treatment and prevention or improvements to existing strategies. Topics: ''oral health'', microbiome, genomics, host-pathogen interactions, oral infections, aetiologic agents, pathogenesis, molecular microbiology systemic diseases, ecology/environmental microbiology, treatment, diagnostics, epidemiology, basic oral microbiology, and taxonomy/systematics. Article types: original articles, notes, review articles, mini-reviews and commentaries
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