behaperet病龈上斑块宏基因组的功能和分类失调。

IF 5.5 2区 医学 Q2 MICROBIOLOGY
Journal of Oral Microbiology Pub Date : 2025-08-29 eCollection Date: 2025-01-01 DOI:10.1080/20002297.2025.2552165
Thanyarat Sapthanakorn, Pitipol Choopong, Wasawat Sermsripong, Chatkoew Boriboonhirunsarn, Chompak Khamwachirapitak, Annop Krasaesin, Pimchanok Sutthiboonyaphan, Nisachon Siripaiboonpong, Rangsini Mahanonda, Paswach Wiriyakijja, George Pelekos, Thantrira Porntaveetus, Supreda Suphanantachat Srithanyarat
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引用次数: 0

摘要

背景:behet病(BD)是一种复杂的自身炎症性疾病,越来越多地与微生物生态失调联系在一起,但特定的微生物特征及其功能后果仍未完全表征。阐明这些改变对于理解双相障碍的发病机制至关重要。目的:利用高分辨率散弹枪宏基因组测序技术,鉴定BD患者与健康对照组(HC)龈上菌斑微生物群的不同微生物群落结构和功能潜力。方法:对18例BD患者和22例hc患者的龈上斑块进行散弹枪宏基因组学分析。分析包括α / β多样性、分类组成和MetaCyc途径丰度,并进行统计比较。结果:尽管年龄和临床依恋水平相似,但与hc相比,BD患者表现出显著增加的α多样性和明显的β多样性。差异丰度分析显示,BD中厌氧和机会性类群(涉及4门28属)的富集,以及19条显著改变的MetaCyc通路,表明BD口腔微生物群中存在实质性的功能重编程。结论:这项高分辨率宏基因组分析揭示了behet病口腔微生物群严重失调,其特征是多样性改变,具有丰富病原体的独特分类特征,以及显著的功能变化。这些全面的微生物改变与驱动双相障碍发病机制的局部和全身炎症过程有关,为诊断生物标志物和靶向治疗提供了潜在的途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Functional and taxonomic dysbiosis of the supragingival plaque metagenome in Behçet's disease.

Background: Behçet's Disease (BD), a complex autoinflammatory disorder, is increasingly linked to microbial dysbiosis, yet the specific microbial signatures and their functional consequences remain incompletely characterized. Elucidating these alterations is crucial for understanding BD pathogenesis.

Objective: To identify distinct microbial community structures and functional potentials in supragingival plaque microbiomes of BD patients versus healthy controls (HC) using high-resolution shotgun metagenomic sequencing.

Methods: Supragingival plaque from 18 BD patients and 22 HCs was subjected to shotgun metagenomics. Analyses included alpha/beta diversity, taxonomic composition, and MetaCyc pathway abundance, with statistical comparisons.

Results: Despite similar age and clinical attachment levels, BD patients exhibited significantly increased alpha diversity and distinct beta diversity compared to HCs. Differential abundance analysis revealed an enrichment of anaerobic and opportunistic taxa in BD (implicating 4 phyla and 28 genera), alongside 19 significantly altered MetaCyc pathways, indicating substantial functional reprogramming within the BD oral microbiome.

Conclusion: This high-resolution metagenomic analysis reveals profound oral microbiome dysbiosis in Behçet's Disease, characterized by altered diversity, a distinct taxonomic signature enriched with pathobionts, and significant functional shifts. These comprehensive microbial alterations are implicated in contributing to the local and systemic inflammatory processes driving BD pathogenesis, offering potential avenues for diagnostic biomarkers and targeted therapies.

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来源期刊
CiteScore
8.00
自引率
4.40%
发文量
52
审稿时长
12 weeks
期刊介绍: As the first Open Access journal in its field, the Journal of Oral Microbiology aims to be an influential source of knowledge on the aetiological agents behind oral infectious diseases. The journal is an international forum for original research on all aspects of ''oral health''. Articles which seek to understand ''oral health'' through exploration of the pathogenesis, virulence, host-parasite interactions, and immunology of oral infections are of particular interest. However, the journal also welcomes work that addresses the global agenda of oral infectious diseases and articles that present new strategies for treatment and prevention or improvements to existing strategies. Topics: ''oral health'', microbiome, genomics, host-pathogen interactions, oral infections, aetiologic agents, pathogenesis, molecular microbiology systemic diseases, ecology/environmental microbiology, treatment, diagnostics, epidemiology, basic oral microbiology, and taxonomy/systematics. Article types: original articles, notes, review articles, mini-reviews and commentaries
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