使用新一代测序分析创伤和龋齿引起的根尖周围病变之间的微生物组差异。

IF 5.5 2区 医学 Q2 MICROBIOLOGY
Journal of Oral Microbiology Pub Date : 2025-09-23 eCollection Date: 2025-01-01 DOI:10.1080/20002297.2025.2560016
Jiyuan Zhan, Yinxue Huang, Xinhui Meng, Yiquan Wang, Jia Liang, Fengjiao Zhu, Rui She, Shanshan Huang, Lijun Huo
{"title":"使用新一代测序分析创伤和龋齿引起的根尖周围病变之间的微生物组差异。","authors":"Jiyuan Zhan, Yinxue Huang, Xinhui Meng, Yiquan Wang, Jia Liang, Fengjiao Zhu, Rui She, Shanshan Huang, Lijun Huo","doi":"10.1080/20002297.2025.2560016","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>While the microbiome of caries-derived periapical lesions has been extensively characterized, the microbial profile of trauma-derived periapical lesions remains poorly understood. This study aimed to characterize the apical microbiome of trauma-derived periapical lesions and identify taxonomic differences between trauma- and caries-derived periapical lesions.</p><p><strong>Methods: </strong>Twenty patients with periapical lesions were enrolled, comprising 10 trauma-derived cases (trauma group) and 10 caries-derived cases (caries group). Microbial samples were collected using sterile paper points inserted into the root canal exudate, followed by DNA extraction and Illumina sequencing of the hypervariable V3-V4 regions of the 16S rRNA gene. Bioinformatic analyses included <i>α</i>-diversity, <i>β</i>-diversity based on Bray-Curtis distance and differential abundance testing (LEfSe method with LDA score ≥ 2.0).</p><p><strong>Results: </strong>Sequencing revealed 36 bacterial phyla and 587 genera across all samples. Trauma group showed significantly greater relative abundance of <i>Campylobacter</i> (<i>P</i> = 0.002) compared to caries group, whereas <i>Prevotella</i> (<i>P</i> = 0.008), <i>Vibrio</i> (<i>P</i> = 0.041) and <i>Filifactor</i> (<i>P</i> = 0.006) exhibited reduced abundance. The core microbiota in the trauma group included <i>Phocaeicola</i>, <i>Porphyromonas</i> and <i>Pyramidobacter</i>, based on relative abundance. LEfSe analysis identified <i>Campylobacter</i> as a biomarker for the trauma group.</p><p><strong>Conclusions: </strong>Trauma-derived periapical lesions exhibited reduced microbial diversity compared to caries-derived periapical lesions, with <i>Campylobacter</i> identified as a potential pathognomonic taxon for trauma-derived periapical lesions.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2560016"},"PeriodicalIF":5.5000,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12459192/pdf/","citationCount":"0","resultStr":"{\"title\":\"Microbiome differences between trauma- and caries-derived periapical lesions using next-generation sequencing.\",\"authors\":\"Jiyuan Zhan, Yinxue Huang, Xinhui Meng, Yiquan Wang, Jia Liang, Fengjiao Zhu, Rui She, Shanshan Huang, Lijun Huo\",\"doi\":\"10.1080/20002297.2025.2560016\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>While the microbiome of caries-derived periapical lesions has been extensively characterized, the microbial profile of trauma-derived periapical lesions remains poorly understood. This study aimed to characterize the apical microbiome of trauma-derived periapical lesions and identify taxonomic differences between trauma- and caries-derived periapical lesions.</p><p><strong>Methods: </strong>Twenty patients with periapical lesions were enrolled, comprising 10 trauma-derived cases (trauma group) and 10 caries-derived cases (caries group). Microbial samples were collected using sterile paper points inserted into the root canal exudate, followed by DNA extraction and Illumina sequencing of the hypervariable V3-V4 regions of the 16S rRNA gene. Bioinformatic analyses included <i>α</i>-diversity, <i>β</i>-diversity based on Bray-Curtis distance and differential abundance testing (LEfSe method with LDA score ≥ 2.0).</p><p><strong>Results: </strong>Sequencing revealed 36 bacterial phyla and 587 genera across all samples. Trauma group showed significantly greater relative abundance of <i>Campylobacter</i> (<i>P</i> = 0.002) compared to caries group, whereas <i>Prevotella</i> (<i>P</i> = 0.008), <i>Vibrio</i> (<i>P</i> = 0.041) and <i>Filifactor</i> (<i>P</i> = 0.006) exhibited reduced abundance. The core microbiota in the trauma group included <i>Phocaeicola</i>, <i>Porphyromonas</i> and <i>Pyramidobacter</i>, based on relative abundance. LEfSe analysis identified <i>Campylobacter</i> as a biomarker for the trauma group.</p><p><strong>Conclusions: </strong>Trauma-derived periapical lesions exhibited reduced microbial diversity compared to caries-derived periapical lesions, with <i>Campylobacter</i> identified as a potential pathognomonic taxon for trauma-derived periapical lesions.</p>\",\"PeriodicalId\":16598,\"journal\":{\"name\":\"Journal of Oral Microbiology\",\"volume\":\"17 1\",\"pages\":\"2560016\"},\"PeriodicalIF\":5.5000,\"publicationDate\":\"2025-09-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12459192/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Oral Microbiology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1080/20002297.2025.2560016\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Oral Microbiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/20002297.2025.2560016","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

背景:虽然龋齿源性根尖周围病变的微生物群已经被广泛表征,但创伤源性根尖周围病变的微生物谱仍然知之甚少。本研究旨在表征创伤源性根尖周围病变的根尖微生物组,并确定创伤源性和龋齿源性根尖周围病变的分类差异。方法:选取20例根尖周围病变患者,其中创伤源性10例(创伤组)和龋源性10例(龋组)。将无菌纸点插入根管分泌物中采集微生物样本,提取DNA并Illumina测序16S rRNA基因高变区V3-V4。生物信息学分析包括α-多样性、基于Bray-Curtis距离的β-多样性和差分丰度检验(LDA评分≥2.0的LEfSe方法)。结果:测序结果显示,所有样品中有36个细菌门和587个属。创伤组弯曲杆菌(Campylobacter)相对丰度显著高于龋齿组(P = 0.002),而Prevotella (P = 0.008)、Vibrio (P = 0.041)和Filifactor (P = 0.006)相对丰度显著降低。根据相对丰度,创伤组的核心微生物群包括Phocaeicola, Porphyromonas和Pyramidobacter。LEfSe分析发现弯曲杆菌是创伤组的生物标志物。结论:与龋齿源性根尖周围病变相比,创伤源性根尖周围病变的微生物多样性较低,弯曲杆菌被确定为创伤源性根尖周围病变的潜在病理分类。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Microbiome differences between trauma- and caries-derived periapical lesions using next-generation sequencing.

Background: While the microbiome of caries-derived periapical lesions has been extensively characterized, the microbial profile of trauma-derived periapical lesions remains poorly understood. This study aimed to characterize the apical microbiome of trauma-derived periapical lesions and identify taxonomic differences between trauma- and caries-derived periapical lesions.

Methods: Twenty patients with periapical lesions were enrolled, comprising 10 trauma-derived cases (trauma group) and 10 caries-derived cases (caries group). Microbial samples were collected using sterile paper points inserted into the root canal exudate, followed by DNA extraction and Illumina sequencing of the hypervariable V3-V4 regions of the 16S rRNA gene. Bioinformatic analyses included α-diversity, β-diversity based on Bray-Curtis distance and differential abundance testing (LEfSe method with LDA score ≥ 2.0).

Results: Sequencing revealed 36 bacterial phyla and 587 genera across all samples. Trauma group showed significantly greater relative abundance of Campylobacter (P = 0.002) compared to caries group, whereas Prevotella (P = 0.008), Vibrio (P = 0.041) and Filifactor (P = 0.006) exhibited reduced abundance. The core microbiota in the trauma group included Phocaeicola, Porphyromonas and Pyramidobacter, based on relative abundance. LEfSe analysis identified Campylobacter as a biomarker for the trauma group.

Conclusions: Trauma-derived periapical lesions exhibited reduced microbial diversity compared to caries-derived periapical lesions, with Campylobacter identified as a potential pathognomonic taxon for trauma-derived periapical lesions.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
8.00
自引率
4.40%
发文量
52
审稿时长
12 weeks
期刊介绍: As the first Open Access journal in its field, the Journal of Oral Microbiology aims to be an influential source of knowledge on the aetiological agents behind oral infectious diseases. The journal is an international forum for original research on all aspects of ''oral health''. Articles which seek to understand ''oral health'' through exploration of the pathogenesis, virulence, host-parasite interactions, and immunology of oral infections are of particular interest. However, the journal also welcomes work that addresses the global agenda of oral infectious diseases and articles that present new strategies for treatment and prevention or improvements to existing strategies. Topics: ''oral health'', microbiome, genomics, host-pathogen interactions, oral infections, aetiologic agents, pathogenesis, molecular microbiology systemic diseases, ecology/environmental microbiology, treatment, diagnostics, epidemiology, basic oral microbiology, and taxonomy/systematics. Article types: original articles, notes, review articles, mini-reviews and commentaries
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信