{"title":"使用新一代测序分析创伤和龋齿引起的根尖周围病变之间的微生物组差异。","authors":"Jiyuan Zhan, Yinxue Huang, Xinhui Meng, Yiquan Wang, Jia Liang, Fengjiao Zhu, Rui She, Shanshan Huang, Lijun Huo","doi":"10.1080/20002297.2025.2560016","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>While the microbiome of caries-derived periapical lesions has been extensively characterized, the microbial profile of trauma-derived periapical lesions remains poorly understood. This study aimed to characterize the apical microbiome of trauma-derived periapical lesions and identify taxonomic differences between trauma- and caries-derived periapical lesions.</p><p><strong>Methods: </strong>Twenty patients with periapical lesions were enrolled, comprising 10 trauma-derived cases (trauma group) and 10 caries-derived cases (caries group). Microbial samples were collected using sterile paper points inserted into the root canal exudate, followed by DNA extraction and Illumina sequencing of the hypervariable V3-V4 regions of the 16S rRNA gene. Bioinformatic analyses included <i>α</i>-diversity, <i>β</i>-diversity based on Bray-Curtis distance and differential abundance testing (LEfSe method with LDA score ≥ 2.0).</p><p><strong>Results: </strong>Sequencing revealed 36 bacterial phyla and 587 genera across all samples. Trauma group showed significantly greater relative abundance of <i>Campylobacter</i> (<i>P</i> = 0.002) compared to caries group, whereas <i>Prevotella</i> (<i>P</i> = 0.008), <i>Vibrio</i> (<i>P</i> = 0.041) and <i>Filifactor</i> (<i>P</i> = 0.006) exhibited reduced abundance. The core microbiota in the trauma group included <i>Phocaeicola</i>, <i>Porphyromonas</i> and <i>Pyramidobacter</i>, based on relative abundance. LEfSe analysis identified <i>Campylobacter</i> as a biomarker for the trauma group.</p><p><strong>Conclusions: </strong>Trauma-derived periapical lesions exhibited reduced microbial diversity compared to caries-derived periapical lesions, with <i>Campylobacter</i> identified as a potential pathognomonic taxon for trauma-derived periapical lesions.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2560016"},"PeriodicalIF":5.5000,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12459192/pdf/","citationCount":"0","resultStr":"{\"title\":\"Microbiome differences between trauma- and caries-derived periapical lesions using next-generation sequencing.\",\"authors\":\"Jiyuan Zhan, Yinxue Huang, Xinhui Meng, Yiquan Wang, Jia Liang, Fengjiao Zhu, Rui She, Shanshan Huang, Lijun Huo\",\"doi\":\"10.1080/20002297.2025.2560016\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>While the microbiome of caries-derived periapical lesions has been extensively characterized, the microbial profile of trauma-derived periapical lesions remains poorly understood. This study aimed to characterize the apical microbiome of trauma-derived periapical lesions and identify taxonomic differences between trauma- and caries-derived periapical lesions.</p><p><strong>Methods: </strong>Twenty patients with periapical lesions were enrolled, comprising 10 trauma-derived cases (trauma group) and 10 caries-derived cases (caries group). Microbial samples were collected using sterile paper points inserted into the root canal exudate, followed by DNA extraction and Illumina sequencing of the hypervariable V3-V4 regions of the 16S rRNA gene. Bioinformatic analyses included <i>α</i>-diversity, <i>β</i>-diversity based on Bray-Curtis distance and differential abundance testing (LEfSe method with LDA score ≥ 2.0).</p><p><strong>Results: </strong>Sequencing revealed 36 bacterial phyla and 587 genera across all samples. Trauma group showed significantly greater relative abundance of <i>Campylobacter</i> (<i>P</i> = 0.002) compared to caries group, whereas <i>Prevotella</i> (<i>P</i> = 0.008), <i>Vibrio</i> (<i>P</i> = 0.041) and <i>Filifactor</i> (<i>P</i> = 0.006) exhibited reduced abundance. The core microbiota in the trauma group included <i>Phocaeicola</i>, <i>Porphyromonas</i> and <i>Pyramidobacter</i>, based on relative abundance. LEfSe analysis identified <i>Campylobacter</i> as a biomarker for the trauma group.</p><p><strong>Conclusions: </strong>Trauma-derived periapical lesions exhibited reduced microbial diversity compared to caries-derived periapical lesions, with <i>Campylobacter</i> identified as a potential pathognomonic taxon for trauma-derived periapical lesions.</p>\",\"PeriodicalId\":16598,\"journal\":{\"name\":\"Journal of Oral Microbiology\",\"volume\":\"17 1\",\"pages\":\"2560016\"},\"PeriodicalIF\":5.5000,\"publicationDate\":\"2025-09-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12459192/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Oral Microbiology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1080/20002297.2025.2560016\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Oral Microbiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/20002297.2025.2560016","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Microbiome differences between trauma- and caries-derived periapical lesions using next-generation sequencing.
Background: While the microbiome of caries-derived periapical lesions has been extensively characterized, the microbial profile of trauma-derived periapical lesions remains poorly understood. This study aimed to characterize the apical microbiome of trauma-derived periapical lesions and identify taxonomic differences between trauma- and caries-derived periapical lesions.
Methods: Twenty patients with periapical lesions were enrolled, comprising 10 trauma-derived cases (trauma group) and 10 caries-derived cases (caries group). Microbial samples were collected using sterile paper points inserted into the root canal exudate, followed by DNA extraction and Illumina sequencing of the hypervariable V3-V4 regions of the 16S rRNA gene. Bioinformatic analyses included α-diversity, β-diversity based on Bray-Curtis distance and differential abundance testing (LEfSe method with LDA score ≥ 2.0).
Results: Sequencing revealed 36 bacterial phyla and 587 genera across all samples. Trauma group showed significantly greater relative abundance of Campylobacter (P = 0.002) compared to caries group, whereas Prevotella (P = 0.008), Vibrio (P = 0.041) and Filifactor (P = 0.006) exhibited reduced abundance. The core microbiota in the trauma group included Phocaeicola, Porphyromonas and Pyramidobacter, based on relative abundance. LEfSe analysis identified Campylobacter as a biomarker for the trauma group.
Conclusions: Trauma-derived periapical lesions exhibited reduced microbial diversity compared to caries-derived periapical lesions, with Campylobacter identified as a potential pathognomonic taxon for trauma-derived periapical lesions.
期刊介绍:
As the first Open Access journal in its field, the Journal of Oral Microbiology aims to be an influential source of knowledge on the aetiological agents behind oral infectious diseases. The journal is an international forum for original research on all aspects of ''oral health''. Articles which seek to understand ''oral health'' through exploration of the pathogenesis, virulence, host-parasite interactions, and immunology of oral infections are of particular interest. However, the journal also welcomes work that addresses the global agenda of oral infectious diseases and articles that present new strategies for treatment and prevention or improvements to existing strategies.
Topics: ''oral health'', microbiome, genomics, host-pathogen interactions, oral infections, aetiologic agents, pathogenesis, molecular microbiology systemic diseases, ecology/environmental microbiology, treatment, diagnostics, epidemiology, basic oral microbiology, and taxonomy/systematics.
Article types: original articles, notes, review articles, mini-reviews and commentaries