Journal of Oral Microbiology最新文献

{"title":"Screening of heat-killed lactic acid bacteria based on inhibitory activity against oral bacteria and effects of oral administration of heat-killed <i>Ligilactobacillus salivarius</i> CP3365 on periodontal health in healthy participants: a double-blinded, randomized, placebo-controlled trial.","authors":"Shinji Sakata,&nbsp;Yukiko Sakamaki,&nbsp;Masahiro Yuki,&nbsp;Tsutomu Sugaya,&nbsp;Tatsuhiko Hirota","doi":"10.1080/20002297.2023.2250649","DOIUrl":"https://doi.org/10.1080/20002297.2023.2250649","url":null,"abstract":"ABSTRACT Objectives The aims of this study were to select heat-killed lactic acid bacteria (HKL) with antibiotic activity and investigate the efficacy of this bacteria in maintaining periodontal parameters in healthy participants. Materials and methods An in vitro evaluation was conducted to assess the inhibitory efficacy of lactic acid bacteria against Porphyromonas gingivalis and Fusobacterium nucleatum subsp. nucleatum. The effects of HKL administration on various parameters (plaque control record, bleeding on probing, and probing pocket depth) were assessed in a randomized, placebo-controlled trial. Participants in the test and placebo groups (n = 32) consumed oral tablets containing placebo or HKL daily for 8 weeks. Oral bacteria in supra-plaque and saliva were identified using 16S rRNA gene community profiling analysis. Results Heat-killed Ligilactobacillus salivarius CP3365 significantly (p < 0.05) decreased the viability of oral bacteria and was selected for clinical trials. Administration of HKL CP3365 significantly (p < 0.05) inhibited increases in each parameter. No changes in the relative abundance of P. gingivalis or F. nucleatum subsp. nucleatum were detected by HKL CP3365, but the relative abundance of oral bacteria (genera Porphyromonas, Fusobacterium, and Haemophilus) was significantly (p < 0.05) decreased. Conclusion HKL CP3365 effectively inhibited oral bacteria growth and was useful for maintaining periodontal health. Clinical Trial Registration [https://www.umin.ac.jp/ctr/index.htm], identifier [UMIN000045656].","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"15 1","pages":"2250649"},"PeriodicalIF":4.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10464545/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10188106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The interplay between oral microbiota, gut microbiota and systematic diseases.","authors":"Xiujun Tan,&nbsp;Yizhong Wang,&nbsp;Ting Gong","doi":"10.1080/20002297.2023.2213112","DOIUrl":"https://doi.org/10.1080/20002297.2023.2213112","url":null,"abstract":"<p><p>Over the past two decades, the importance of microbiota in health and disease has become evident. The human gut microbiota and oral microbiota are the largest and second-largest microbiome in the human body, respectively, and they are physically connected as the oral cavity is the beginning of the digestive system. Emerging and exciting evidence has shown complex and important connections between gut microbiota and oral microbiota. The interplay of the two microbiomes may contribute to the pathological processes of many diseases, including diabetes, rheumatoid arthritis, nonalcoholic fatty liver disease, inflammatory bowel disease, pancreatic cancer, colorectal cancer, and so on. In this review, we discuss possible routes and factors of oral microbiota to affect gut microbiota, and the contribution of this interplay between oral and gut microbiota to systemic diseases. Although most studies are association studies, recently, there have been increasing mechanistic investigations. This review aims to enhance the interest in the connection between oral and gut microbiota, and shows the tangible impact of this connection on human health.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"15 1","pages":"2213112"},"PeriodicalIF":4.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/1f/a4/ZJOM_15_2213112.PMC10187086.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10192809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of oral microbiota in non-vital teeth and clinically intact external surface from patients with severe periodontitis using Nanopore sequencing: a case study.","authors":"Alessio Buonavoglia,&nbsp;Francesco Pellegrini,&nbsp;Gianvito Lanave,&nbsp;Georgia Diakoudi,&nbsp;Maria Stella Lucente,&nbsp;Fausto Zamparini,&nbsp;Michele Camero,&nbsp;Maria Giovanna Gandolfi,&nbsp;Vito Martella,&nbsp;Carlo Prati","doi":"10.1080/20002297.2023.2185341","DOIUrl":"https://doi.org/10.1080/20002297.2023.2185341","url":null,"abstract":"<p><p>Periodontal diseases include a wide range of pathological conditions, damaging the supporting structures of the teeth. Origin and propagation of periodontal disease is believed to be caused by dysbiosis of the commensal oral microbiota. The aim of this study was to evaluate the presence of bacteria in the pulp cavity of teeth with severe periodontal disease with clinically intact external surface. Periodontal (P) and endodontic (E) tissue samples of root canals from six intact teeth of three patients were sampled for analysis of microbial population using Nanopore technology. <i>Streptococcus</i> was the predominant genus in E samples. <i>Porphyromonas</i> (33.4%, <i>p</i> = 0.047), <i>Tannerella</i> (41.7%, <i>p</i> = 0.042), and <i>Treponema</i> (50.0%, <i>p</i> = 0.0064) were significantly more present in P than in E samples. Some samples (E6 and E1) exhibited a remarkable difference in terms of microbial composition, whilst <i>Streptococcus</i> was a common signature in samples E2 to E5, all which were obtained from the same patient. In conclusion, bacteria were identified on both the root surface and the root canal system, thus demonstrating the possibility of bacteria to spread directly from the periodontal pocket to the root canal system even in the absence of crown's loss of integrity.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"15 1","pages":"2185341"},"PeriodicalIF":4.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9987744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9076054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mode of killing determines the necrotrophic response of oral bacteria.","authors":"Naiera Zayed,&nbsp;Joana Figueiredo,&nbsp;Wannes Van Holm,&nbsp;Nico Boon,&nbsp;Kristel Bernaerts,&nbsp;Wim Teughels","doi":"10.1080/20002297.2023.2184930","DOIUrl":"https://doi.org/10.1080/20002297.2023.2184930","url":null,"abstract":"<p><strong>Background: </strong>Bacteria respond to changes in their environment, such as nutrient depletion and antimicrobials exposure. Antimicrobials result not only in bacterial death, but also have a hand in determining species abundances and ecology of the oral biofilms. Proximity of dead bacterial cells to living ones is an important environmental change or stress factor. Dead bacteria represent high concentrations of nutrients, such as proteins, lipids, sugars, and nucleic acids. Living bacteria can use these biomasses as a nutrients source, which is termed necrotrophy.</p><p><strong>Aim: </strong>This study investigates the effect of exposing living oral bacteria (planktonic and biofilms) to their dead siblings after being killed by heat or hydrogen peroxide.</p><p><strong>Results: </strong>Tested bacterial species showed different responses towards the dead cells, depending on the mode of killing, the nutritional value of the culture media, and the the dead cells density. The multispecies oral biofilms showed different responses towards the supplementation of dead cells during biofilm development, while matured biofilms were more resilient.</p><p><strong>Conclusion: </strong>This study indicates that dead bacteria resulting from antiseptics use may imbalance the nutrient availability in the oral cavity, resulting in overgrowth of opportunistic species, and hence ecological changes in oral communities, or introducing new bacterial phenotypes.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"15 1","pages":"2184930"},"PeriodicalIF":4.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10013485/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9129622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First water safety plan approach applied to a Dental Clinic complex: identification of new risk factors associated with <i>Legionella</i> and <i>P. aeruginosa</i> contamination, using a novel sampling, maintenance and management program.","authors":"Federica Marino,&nbsp;Marta Mazzotta,&nbsp;Maria Rosaria Pascale,&nbsp;Carlo Derelitto,&nbsp;Luna Girolamini,&nbsp;Sandra Cristino","doi":"10.1080/20002297.2023.2223477","DOIUrl":"https://doi.org/10.1080/20002297.2023.2223477","url":null,"abstract":"<p><p>Dental unit waterlines (DUWLs) represent a complex environment able to promote microbial contamination, due to functional, mechanical and practical risk factors. According to a water safety plan approach, the main goal is to preserve the health of dentists, dental staff and patients. The aim of this study is to develop a DUWLs water safety plan that is able to support correct and effective maintenance and disinfection procedures. Three different water systems serve 60 dental chairs: (i) water that comes directly from municipal water (Type A), (ii) water supplied by municipal water and water bottles (Type B) and (iii) water supplied only via water bottles (Type C). For each type, <i>Legionella</i> and <i>Pseudomonas aeruginosa</i> contamination was studied, by applying a new sampling scheme, based on separate sampling from water bottles, cup filler and handpieces. Type B DUWL is the only type of DUWL contaminated by <i>L. pneumophila</i> (ST 59) and <i>L. anisa</i> (mean contamination: 608.33 ± 253.33 cfu/L) detected in cup filler and handpieces, as well as the high presence of <i>P. aeruginosa</i> (44.42 ± 13.25 cfu/100 mL). Two subsequent shock treatments and resampling procedures were performed by increasing disinfectant dosage and contact time and removing some DUWL components linked to biofilm growth in DUWLs. A significant reduction of contamination was obtained for both microorganisms (<i>Legionella</i> spp.: -100%, <i>p</i> < 0.001 and <i>P. aeruginosa</i>: -99.86%, <i>p</i> = 0.006). The sampling strategy proposed allows us to identify the source of contamination and better focus on the maintenance and disinfection procedures. DUWLs represent an environment that requires a multidisciplinary approach, combining the knowledge of all DUWL components to correct procedures that are able to preserve the health of personnel and patients, as well as guaranteeing DUWLs' safe functionality.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"15 1","pages":"2223477"},"PeriodicalIF":4.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/df/c3/ZJOM_15_2223477.PMC10281406.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10545145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Porphyromonas gingivalis</i> diffusible signaling molecules enhance <i>Fusobacterium nucleatum</i> biofilm formation <i>via</i> gene expression modulation.","authors":"Yukiko Yamaguchi-Kuroda,&nbsp;Yuichiro Kikuchi,&nbsp;Eitoyo Kokubu,&nbsp;Kazuyuki Ishihara","doi":"10.1080/20002297.2023.2165001","DOIUrl":"https://doi.org/10.1080/20002297.2023.2165001","url":null,"abstract":"<p><strong>Background: </strong>Periodontitis is caused by a dysbiotic shift in the dental plaque microbiome. <i>Fusobacterium nucleatum</i> is involved in the colonization of <i>Porphyromonas gingivalis</i>, which plays a key role in dysbiosis, via coaggregation and synergy with this microorganism.</p><p><strong>Aim: </strong>We investigated the effect of diffusible signaling molecules from <i>P. gingivalis</i> ATCC 33277 on <i>F. nucleatum</i> TDC 100 to elucidate the synergistic mechanisms involved in dysbiosis.</p><p><strong>Methods: </strong>The two species were cocultured separated with an 0.4-µm membrane in tryptic soy broth, and <i>F. nucleatum</i> gene expression profiles in coculture with <i>P. gingivalis</i> were compared with those in monoculture.</p><p><strong>Results: </strong>RNA sequencing revealed 139 genes differentially expressed between the coculture and monoculture. The expression of 52 genes was upregulated, including the coaggregation ligand-coding gene. Eighty-seven genes were downregulated. Gene Ontology analysis indicated enrichment for the glycogen synthesis pathway and a decrease in de novo synthesis of purine and pyrimidine.</p><p><strong>Conclusion: </strong>These results indicate that diffusible signaling molecules from <i>P. gingivalis</i> induce metabolic changes in <i>F. nucleatum</i>, including an increase in polysaccharide synthesis and reduction in de novo synthesis of purine and pyrimidine. The metabolic changes may accelerate biofilm formation by <i>F. nucleatum</i> with <i>P. gingivalis</i>. Further, the alterations may represent potential therapeutic targets for preventing dysbiosis.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"15 1","pages":"2165001"},"PeriodicalIF":4.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9848294/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10583619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A mouthwash formulated with o-cymen-5-ol and zinc chloride specifically targets potential pathogens without impairing the native oral microbiome in healthy individuals.","authors":"Javier Pascual,&nbsp;Javier Mira Otal,&nbsp;Daniel Torrent-Silla,&nbsp;Manuel Porcar,&nbsp;Cristina Vilanova,&nbsp;Fernando Vivancos Cuadras","doi":"10.1080/20002297.2023.2185962","DOIUrl":"https://doi.org/10.1080/20002297.2023.2185962","url":null,"abstract":"<p><strong>Background: </strong>Many antimicrobial compounds in mouthwashes can have a negative impact on the oral microbiome. O-cymen-5-ol, a compound derived from a phytochemical, has a targeted mode of action and is being used as an alternative. However, its effect on the native oral microbiome is unknown.</p><p><strong>Aim: </strong>To assess the effect of a mouthwash formulated with o-cymen-5-ol and zinc chloride on the oral microbiome of healthy individuals.</p><p><strong>Methods: </strong>A mouthwash formulated with o-cymen-5-ol and zinc chloride was administered to a cohort of 51 volunteers for 14 days, while another cohort of 49 volunteers received a placebo. The evolution of the oral microbiome in both groups was analysed using a metataxonomic approach.</p><p><strong>Results: </strong>Analysis of the oral microbiome showed that the mouthwash selectively targeted potential oral pathogens while maintaining the integrity of the rest of the microbiome. Specifically, the relative abundance of several potentially pathogenic bacterial taxa, namely <i>Fusobacteriota</i>, <i>Prevotella</i>, <i>Actinomyces</i>, <i>Granulicatella</i>, <i>Abiotrophia</i>, <i>Lautropia</i>, <i>Lachnoanaerobaculum</i>, <i>Eubacterium</i> (nodatum group) and <i>Absconditabacteriales</i> (SR1) decreased, while the growth of <i>Rothia</i>, a nitrate-reducing bacterium beneficial for blood pressure, was stimulated.</p><p><strong>Conclusions: </strong>The use of o-cymen-5-ol and zinc chloride as antimicrobial agents in oral mouthwashes is a valuable alternative to classical antimicrobial agents.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"15 1","pages":"2185962"},"PeriodicalIF":4.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9987754/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9076059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metataxonomic and metaproteomic profiling of the oral microbiome in oral lichen planus - a pilot study.","authors":"Maria Bankvall,&nbsp;Miguel Carda-Diéguez,&nbsp;Alex Mira,&nbsp;Anders Karlsson,&nbsp;Bengt Hasséus,&nbsp;Roger Karlsson,&nbsp;Jairo Robledo-Sierra","doi":"10.1080/20002297.2022.2161726","DOIUrl":"https://doi.org/10.1080/20002297.2022.2161726","url":null,"abstract":"<p><strong>Background: </strong>A growing body of evidence demonstrates a different bacterial composition in the oral cavity of patients with oral lichen planus (OLP).</p><p><strong>Patients and methods: </strong>Buccal swab samples were collected from affected and non-affected sites of six patients with reticular OLP and the healthy oral mucosa of six control subjects. 16S rRNA gene MiSeq sequencing and mass spectrometry-based proteomics were utilised to identify the metataxonomic and metaproteomic profiles of the oral microbiome in both groups.</p><p><strong>Results: </strong>From the metataxonomic analysis, the most abundant species in the three subgroups were Streptococcus oralis and Pseudomonas aeruginosa, accounting for up to 70% of the total population. Principal Coordinates Analysis showed differential clustering of samples from the healthy and OLP groups. ANCOM-BC compositional analysis revealed multiple species (including P. aeruginosa and several species of Veillonella, Prevotella, Streptococcus and Neisseria) significantly over-represented in the control group and several (including Granulicatella elegans, Gemella haemolysans and G. parahaemolysans) in patients with OLP. The metaproteomic data were generally congruent and revealed that several Gemella haemolysans-belonging peptidases and other proteins with inflammatory and virulence potential were present in OLP lesions.</p><p><strong>Conclusion: </strong>Our data suggest that several bacterial species are associated with OLP. Future studies with larger cohorts should be conducted to determine their role in the aetiology of OLP and evaluate their potential as disease biomarkers.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"15 1","pages":"2161726"},"PeriodicalIF":4.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9809343/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10843681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the biomedical potential of a novel modified glass ionomer cement against the pandrug-resistant oral pathogen <i>Candida albicans</i> SYN-01.","authors":"Nessma A El Zawawy,&nbsp;Samy El-Safty,&nbsp;El-Refaie Kenawy,&nbsp;Sara Ibrahim Salem,&nbsp;Sameh S Ali,&nbsp;Yehia A-G Mahmoud","doi":"10.1080/20002297.2023.2195741","DOIUrl":"https://doi.org/10.1080/20002297.2023.2195741","url":null,"abstract":"<p><p>Dental caries is an infectious disease that is a major concern for dentists. <i>Streptococci</i> and <i>Lactobacilli</i> were long thought to be the primary etiology responsible for caries. <i>Candida albicans</i> with acidogenic and aciduric characteristics has recently been implicated in the onset and progression of cariogenic lesions. Moreover, due to the increased resistance to common antimicrobials, the discovery of innovative candidates is in high demand. Therefore, our study might be the first report that explores the efficacy of glass ionomer cement (GIC) incorporated with a newly modified carboxylated chitosan derivative (CS-MC) against multidrug-resistant (MDR) and/or pandrug resistant (PDR) <i>C. albicans</i> isolated from the oral cavity. In this work, four CS-MC-GIC groups with different concentrations were formulated. Group four (CS-MC-GIC-4) gave a significant performance as an anticandidal agent against selected PDR <i>Candida</i> strain, with an obvious decrease in its cell viability and high antibiofilm activity. It also, enhanced all the mechanical properties and supports cell viability of Vero cells as a nontoxic compound. Moreover, CS-MC-GIC-4 inhibited neuraminidases completely, which might provide a novel mechanism to prevent dental/oral infections. Thus, findings in this study open up new prospect of the utilization of CS-MC-GIC as a novel dental filling material against oral drug-resistant <i>Candida</i>.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"15 1","pages":"2195741"},"PeriodicalIF":4.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10064826/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9241704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Saliva microbiome alterations in dental fluorosis population.","authors":"Shanshan Liu,&nbsp;Qiangsheng Song,&nbsp;Chenchen Zhang,&nbsp;Mengwan Li,&nbsp;Zhenzhen Li,&nbsp;Yudong Liu,&nbsp;Li Xu,&nbsp;Xiaofei Xie,&nbsp;Lili Zhao,&nbsp;Rongxiu Zhang,&nbsp;Qinglong Wang,&nbsp;Guojin Zeng,&nbsp;Yifan Zhang,&nbsp;Kai Zhang","doi":"10.1080/20002297.2023.2180927","DOIUrl":"https://doi.org/10.1080/20002297.2023.2180927","url":null,"abstract":"<p><strong>Background: </strong>We aimed to explore saliva microbiome alterations in dental fluorosis population.</p><p><strong>Methods: </strong>The prevalence of dental fluorosis was examined in 957 college students. Dean's fluorosis index was used to evaluate the dental fluorosis status. Changes in the composition of the salivary microbiome were assessed in a subset of these patients (100 healthy controls, 100 dental fluorosis patients).</p><p><strong>Results: </strong>Dental fluorosis affected 47% of the student sample, and incidence was unrelated to gender. Compared with healthy controls, the microbiota of patients with dental fluorosis exhibited increased diversity, with increased abundance of <i>Treponema lecithinolyticum, Vibrio metschnikovii</i>, <i>Cupriavidus pauculus</i>, <i>Pseudomonas</i>, <i>Pseudomonadaceae</i>, <i>Pseudomonadales</i>, and decreased abundance of <i>Streptococcus mutans</i>, <i>Streptococcus sanguinis</i>, <i>Gemella</i>, and <i>Staphylococcales</i>. Function analyses showed increases in arginine biosynthesis in patients affected by dental fluorosis, together with reductions in amino sugar and nucleotide sugar metabolism, fructose and mannose metabolism, and starch and sucrose metabolism.</p><p><strong>Conclusions: </strong>These results suggest that there are striking differences in salivary microbiome between healthy controls and dental fluorosis patients. Dental fluorosis may contribute to periodontitis and systemic lung diseases. There is a need for cohort studies to determine whether altering the salivary microbiota in dental fluorosis patients can alter the development of oral or systemic diseases.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"15 1","pages":"2180927"},"PeriodicalIF":4.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9946311/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9356945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}