Journal of Oral Microbiology最新文献

{"title":"Streptococcus mutans exacerbates gut microbiota dysbiosis in SHANK3 ^<b>-/-</b> autism model mice via the oral-gut axis.","authors":"Wenyan Huang, Shaohong Wang, Yan Zhang, Min Gao, Ning Zhong, Changhua Hao, Lal Pathak Janak, Lijing Wang, Si Meng, Wanghong Zhao, Sujuan Zeng","doi":"10.1080/20002297.2026.2681259","DOIUrl":"10.1080/20002297.2026.2681259","url":null,"abstract":"<p><strong>Background and objective: </strong>Autism spectrum disorder (ASD) is associated with gut microbiota dysbiosis, yet the impact of oral pathobiont translocation via the oral-gut axis remains unclear. This study investigated how <i>Streptococcus mutans</i> (<i>S. mutans</i>), a primary cariogenic pathogen, influences gut microbial structure and function in an ASD mouse model.</p><p><strong>Methods: </strong>SHANK3 knockout (SHANK3^-/-) and wild-type (WT) mice were divided into four groups: WT control, WT <i>S. mutans</i>-gavaged (WT-S.m), SHANK3^-/- control, and SHANK3^-/- <i>S. mutans</i>-gavaged (SHANK3-S.m). Mice were gavaged with <i>S. mutans</i> UA159 twice weekly for five weeks, followed by fecal metagenomic sequencing (<i>n</i> = 6 per group).</p><p><strong>Results: </strong><i>S. mutans</i> translocated to the gut in both gavaged groups but did not achieve enhanced colonization in SHANK3^-/- mice. <i>S. mutans</i> gavage significantly altered the gut microbiota structure in both WT and SHANK3^-/- mice. In the ASD model, <i>S. mutans</i> gavage led to a significant enrichment of potential pathobionts (e.g. <i>Duncaniella dubosii</i>, <i>Muribaculum gordoncarteri</i>) and a decrease in beneficial bacteria (e.g. <i>Bacteroides caecimuris, Bacteroides faecium</i>). LEfSe analysis identified <i>Parascardovia denticolens</i> and <i>Bacteroides heparinolyticus</i> as specific biomarkers for the SHANK3-S.m group. Microbial networks showed reduced stability in SHANK3-S.m mice, with <i>Enterocloster bolteae</i> as a key node. Functional analysis revealed suppressed butanoate metabolism and enhanced neuroinflammation-related pathways.</p><p><strong>Conclusion: </strong>Although <i>S. mutans</i> colonized only transiently, it provoked exacerbated ecological instability and pro-inflammatory metabolic alterations in ASD model mice, underscoring the role of the oral-gut-brain axis in ASD.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2681259"},"PeriodicalIF":6.7,"publicationDate":"2026-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13237793/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blueberry leaf polyphenols suppress biofilm formation and restore oral microbial homeostasis for caries control.","authors":"Xuanlan Wu, Heng Xiao, Yanbing Chen, Chunqing Wu, Qiyuan Xie, Yu Gu, Xiaofei Qin","doi":"10.1080/20002297.2026.2681284","DOIUrl":"10.1080/20002297.2026.2681284","url":null,"abstract":"<p><strong>Background: </strong>Dental caries is a dysbiotic biofilm-induced disease driven by <i>Streptococcus mutans</i> (<i>S. mutans</i>). Blueberry leaf polyphenols (BLP) are plant-derived polyphenols exhibiting antimicrobial properties. However, their effects on <i>S. mutans</i> remain incompletely understood.</p><p><strong>Methods: </strong>The composition of BLP was determined by ultrahigh-pressure liquid chromatography-triple quadrupole tandem mass spectrometer (UPLC-MS/MS). The minimum inhibitory concentration (MIC) and acid suppression activity were determined. In addition, the effect on biofilm was investigated by adhesion and aggregation assay, crystal violet assay, glycosyltransferases (GTF) activity, extracellular polysaccharides (EPS) quantification, scanning electron microscope (SEM) images and qRT-PCR. Furthermore, we carried out a rat caries model, and microbiome analyzes were performed by 16S rRNA sequencing.</p><p><strong>Results: </strong>UPLC-MS/MS analysis identified a range of compounds in BLP, including quercetin and chlorogenic acid. The MIC of BLP was 500 μg/mL, and it inhibited the acid production of <i>S. mutans</i>. Importantly, BLP decreased the sucrose-dependent adhesion and its auto-aggregation, inhibited the activity of GTF, resulting in reduced EPS and down-regulated genes like <i>gtfB, comA and gbpB</i>. In addition, it inhibited dental caries in rats and ameliorated the microbiota dysbiosis associated with caries.</p><p><strong>Conclusions: </strong>BLP combats caries by inhibiting <i>S. mutans</i> growth, biofilm formation and restoring the oral microbiota.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2681284"},"PeriodicalIF":6.7,"publicationDate":"2026-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13237790/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fibrinogen was associated with subgingival microbiome in periodontal diseases: a pilot study.","authors":"Aopeng Zhang, Ruolan Du, Lei Lei, Jianan Liu, Gao Sun, Rui Wang, Tao Hu, Ran Cheng","doi":"10.1080/20002297.2026.2681264","DOIUrl":"10.1080/20002297.2026.2681264","url":null,"abstract":"<p><strong>Background: </strong>Periodontal diseases are associated with complex interactions among inflammatory responses, microbial dysbiosis, and clinical periodontal parameters. However, the relationships among inflammatory biomarkers, microbial biomarkers, and clinical parameters in gingival crevicular fluid (GCF) remain to be further clarified.</p><p><strong>Objective: </strong>To explore the interactions among inflammatory biomarkers, microbial biomarkers, and clinical parameters in gingival crevicular fluid in periodontal diseases.</p><p><strong>Design: </strong>In this cross-sectional study, GCF and subgingival plaque were collected from healthy, gingivitis, and periodontitis participants. Levels of fibrinogen, fibrin (measured as fibrin degradation products (FDP)), Interleukin (IL)-1β, IL-17, Matrix metallopeptidase (MMP)8, and MMP9 were measured by enzyme-linked immunosorbent assay. The subgingival microbiome was analyzed using 16S rRNA gene sequencing.</p><p><strong>Results: </strong>Among all biomarkers, fibrinogen was the most sensitive biomarker detected in GCF. Levels of fibrinogen were higher in the gingivitis (<i>p </i>= 0.024) and periodontitis (<i>p </i>= 0.002) groups than in the healthy group. Positive correlations were found between fibrinogen and numerous subgingival microorganisms, such as <i>Tannerella forsythia</i>, <i>Treponema denticola</i>, <i>Porphyromonas gingivalis</i>, and <i>Filifactor alocis</i>. Fibrinogen was the only GCF marker that could differentiate between healthy and gingivitis individuals. Fibrinogen and its combination with specific subgingival microorganisms may be potential markers indicating gingivitis and periodontitis.</p><p><strong>Conclusions: </strong>Compared with IL-1β, IL-17, MMP8 and MMP9, fibrinogen in GCF demonstrated distinct associations with clinical parameters and subgingival microbiome in periodontal diseases.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2681264"},"PeriodicalIF":6.7,"publicationDate":"2026-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13235256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The association between tongue features and tongue coating microbiota and gastrointestinal cancer: a systematic review and meta-analysis.","authors":"Xin Gao, Yuan Zou, Jianming Fang, Xiaqiu Wu, Weifang Zheng, Yongsheng Zhang, Wen Hao","doi":"10.1080/20002297.2026.2681268","DOIUrl":"10.1080/20002297.2026.2681268","url":null,"abstract":"<p><strong>Background: </strong>Gastrointestinal (GI) cancers pose a significant health burden, highlighting the need for non-invasive biomarkers. Tongue inspection, a traditional diagnostic method in Chinese medicine, has been increasingly quantified via imaging and microbiome analysis.</p><p><strong>Objective: </strong>This review synthesizes evidence on tongue features and coating microbiota in GI cancer detection.</p><p><strong>Design: </strong>We systematically searched PubMed, EMBASE, Cochrane and Chinese databases until July 2025 for case-control or cohort studies comparing tongue characteristics or microbiota between GI cancer patients and healthy controls. Data were pooled using fixed- or random-effects models.</p><p><strong>Results: </strong>Sixteen studies (<i>n</i> = 4,994) were included. GI cancer patients showed significantly higher rates of abnormal tongue body morphology (OR = 5.33, 95% CI 3.26-8.72), abnormal tongue body color (OR = 17.85, 95% CI 7.01-45.54), abnormal tongue coating texture (OR = 5.98, 95% CI 4.02-8.91) and abnormal tongue coating color (OR = 3.24, 95% CI 2.00-5.26) versus controls. Although α-diversity did not differ, certain taxa (e.g. Actinobacteria, Prevotella_7) were reduced in cancer patients. Subgroup analyses by cancer type showed generally consistent directions of association for abnormal tongue manifestations in gastric, colorectal and esophageal cancers, despite significant heterogeneity.</p><p><strong>Conclusion: </strong>Abnormal tongue features and specific microbial shifts are associated with GI cancers, suggesting potential non-invasive tools for early detection. However, due to heterogeneity and methodological limitations, further large-scale prospective studies are needed for validation.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2681268"},"PeriodicalIF":6.7,"publicationDate":"2026-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13231814/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148163377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A prospective cohort study exploring salivary microbial predictors of white spot lesions during fixed orthodontic treatment.","authors":"Zijian Cheng, Lu Gao, Dingwen Luo, Chunsheng Bi, Qianming Chen, Xiaoyan Chen","doi":"10.1080/20002297.2026.2679805","DOIUrl":"10.1080/20002297.2026.2679805","url":null,"abstract":"<p><strong>Introduction: </strong>White spot lesions (WSLs) are a common adverse outcome of fixed orthodontic treatment, prompting the search for salivary microbial predictors for early risk identification.</p><p><strong>Materials and methods: </strong>Sixty-four patients undergoing fixed metal self-ligating orthodontic treatment were categorized into WSL and non-WSL groups based on lesion development after 12 months. Clinical indices (DI-S, CI-S, OHI-S, GI) and unstimulated whole saliva samples were collected at baseline and after 12 months. The salivary microbiome was analyzed using 16S rRNA gene sequencing.</p><p><strong>Results: </strong>The prevalence of WSLs was 54.7% at the patient level. Clinical indices did not differ significantly between groups. Although overall microbial community structure was similar at baseline, several taxa were enriched in patients who later developed WSLs, including <i>Actinomyces, Rothia and Granulicatella.</i> After 12 months, the WSL group showed reduced phylogenetic diversity and a microbial profile enriched in acidogenic and anaerobic taxa. Functional prediction indicated increased carbohydrate metabolism and fermentative pathways in WSL patients.</p><p><strong>Conclusions: </strong>WSL development during fixed orthodontic treatment was associated with baseline salivary microbial differences and treatment-related ecological shifts. Salivary microbiome profiling may help identify patients at higher risk and support early risk stratification and preventive strategies in orthodontic care.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2679805"},"PeriodicalIF":6.7,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13224688/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148150235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transient restructuring of the active oral resistome during probiotic <i>Streptococcus salivarius</i> K12 colonization in a 3D polymicrobial biofilm model.","authors":"Nadeeka S Udawatte, Chun Liu, Reuben Staples, Pingping Han, Purnima S Kumar, Thiruma V Arumugam, Sašo Ivanovski, Chaminda Jayampath Seneviratne","doi":"10.1080/20002297.2026.2680793","DOIUrl":"10.1080/20002297.2026.2680793","url":null,"abstract":"<p><strong>Background: </strong>The oral cavity harbours a complex and transcriptionally active antibiotic resistance gene (ARG) reservoir shaped by polymicrobial biofilm ecology. Whether probiotic-mediated ecological modulation can remodel the active resistome without promoting horizontal gene transfer remains poorly understood.</p><p><strong>Objective: </strong>To investigate the impact of <i>Streptococcus salivarius</i> K12 (Ssk12) colonisation on active resistome dynamics within saliva derived polymicrobial biofilms and determine whether probiotic driven ecological restructuring transiently alters resistance-associated transcriptional signatures.</p><p><strong>Design: </strong>Saliva-derived polymicrobial biofilms were established on three-dimensional melt electrowritten poly(ε-caprolactone) (MEW-mPCL) scaffolds and exposed to Ssk12. Metatranscriptomic profiling was performed across four time points (Baseline, Day 4, Day 7, and Day 10), complemented by quantitative PCR validation and ARG-mobile genetic element (MGE) co-localisation analysis to characterise resistome restructuring during probiotic colonisation and decolonisation.</p><p><strong>Results: </strong>Baseline biofilms contained 27 ARGs spanning 16 antibiotic classes, predominantly <i>ermB</i>, <i>tet(M)</i>, and <i>tet(W)</i>. During peak Ssk12 colonisation (Days 4-7), total ARG abundance declined to approximately 17% of baseline levels, with marked reductions in efflux-associated and β-lactam/fluoroquinolone resistance-associated transcripts. Partial resistome recovery occurred by Day 10 (~32% of baseline), indicating reversible ecological modulation rather than permanent dysbiotic restructuring. ARG dynamics were primarily reshaped by ARG-bearing taxa rather than enrichment of high-confidence putatively mobile resistance determinants.</p><p><strong>Conclusions: </strong><i>S. salivarius</i> K12 transiently remodelled the transcriptionally active oral resistome within structured polymicrobial biofilms without evidence of enhanced putative horizontal resistance gene mobilisation. These findings support a proof-of-concept model in which probiotic driven ecological restructuring may create a transient resistome state potentially associated with altered responsiveness to selected antibiotic classes.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2680793"},"PeriodicalIF":6.7,"publicationDate":"2026-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13224720/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148150179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of 16S rRNA gene amplicon and whole-genome shotgun metagenomic sequencing for subgingival oral microbiome profiling.","authors":"Jung Hwa Park, Jin Chung, Hyo-Jung Lee, Hee Sam Na","doi":"10.1080/20002297.2026.2679807","DOIUrl":"10.1080/20002297.2026.2679807","url":null,"abstract":"<p><strong>Background: </strong>Periodontitis is a chronic inflammatory disease driven by a dysbiotic subgingival microbiome. While 16S rRNA gene amplicon sequencing is widely used, whole-genome shotgun (WGS) metagenomics is increasingly applied for higher taxonomic and functional resolution.</p><p><strong>Objective: </strong>The aim of this study was to directly compare 16S rRNA gene amplicon (V1-V2) sequencing and WGS metagenomic sequencing using matched subgingival plaque samples from patients with periodontitis.</p><p><strong>Methods: </strong>Subgingival plaque samples from 28 patients with periodontitis were analyzed using both 16S rRNA gene amplicon (V1-V2) sequencing and WGS metagenomics. Taxonomic composition, microbial diversity, differential abundance and functional analysis were compared across platforms.</p><p><strong>Results: </strong>WGS generated markedly higher read counts than 16S rRNA gene amplicon but showed wide variability in non-human reads, whereas 16S rRNA gene amplicon yielded a consistent proportion of non-chimeric reads. High taxonomic overlap was observed at the phylum level but declined at higher taxonomic ranks. WGS preferentially detected taxa such as <i>Actinomyces</i>, <i>Corynebacterium</i> and <i>Olsenella</i>, while the 16S rRNA gene amplicon more frequently captured <i>Saccharibacteria</i> (TM7) and low-abundance taxa. Core genera, including <i>Rothia</i>, <i>Neisseria</i> and <i>Cardiobacterium</i> showed comparable abundance patterns across platforms. When patients were grouped depending on probing pocket depth (PPD), LEfSe analysis resulted in platform-specific enrichment patterns. Functional analyses revealed shared central pathways, such as pyruvate metabolism, while 16S-based PICRUSt2 emphasized reductive and degradative pathways and WGS-based HUMAnN highlighted oxidative and biosynthetic pathways. Notably, WGS-based functional profiles were strongly influenced by microbial read depth.</p><p><strong>Conclusions: </strong>This comparative analysis demonstrates that 16S rRNA gene amplicon (V1-V2) sequencing and WGS both robustly capture core subgingival microbial signatures. While WGS provides higher species-level and functional resolution, the resolution was strongly constrained by microbial read depth in host-rich subgingival samples. These findings provide practical guidance for selecting appropriate sequencing strategies and optimizing sample preparation when designing WGS-based periodontal microbiome studies.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2679807"},"PeriodicalIF":6.7,"publicationDate":"2026-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13218309/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148138451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Periodontal status and microbial profiles across the clinical spectrum of liver cirrhosis.","authors":"J B O Batista, E H de Souza Oliveira, L A M Penteado, V X Nascimento, S Matsumura, J M M Silva, D F Nóbrega, B Retamal-Valdes, M Feres, S M S Ferreira","doi":"10.1080/20002297.2026.2678639","DOIUrl":"10.1080/20002297.2026.2678639","url":null,"abstract":"<p><strong>Objectives: </strong>Periodontitis is common in patients with cirrhosis, but this association remains underexplored. This cross-sectional study aimed to assess periodontal conditions and characterize periodontal biofilm composition in individuals with liver cirrhosis.</p><p><strong>Methods: </strong>Adults with liver cirrhosis were enrolled. Periodontal parameters were recorded, and supragingival and subgingival biofilm samples were collected. Forty bacterial species were quantified using checkerboard DNA-DNA hybridization. Findings were stratified by Child-Pugh class and MELD score.</p><p><strong>Results: </strong>Forty-five patients were included; 88.9% exhibited periodontal disease (57.8% gingivitis on a reduced periodontium, 31.1% periodontitis), and 11.1% were periodontally healthy. Periodontal diagnosis was not associated with liver-disease severity (<i>p</i> > 0.05). Gingivitis showed the highest supragingival bacterial load, with enrichment of orange- and red-complex pathogens, particularly <i>Tannerella forsythia</i>. Patients with compensated liver disease (Child-Pugh A; MELD < 15) showed higher counts of <i>Aggregatibacter actinomycetemcomitans</i> and <i>Fusobacterium nucleatum</i> ssp. <i>nucleatum</i> (<i>p</i> < 0.05). Among patients with periodontitis, higher subgingival proportions of red-complex pathogens were observed in those with MELD < 15.</p><p><strong>Conclusions: </strong>Periodontal disease was highly prevalent in patients with cirrhosis. Although periodontal diagnosis was not associated with liver-disease severity, distinct microbiological patterns were observed. These findings highlight the potential relevance of periodontal monitoring in patients with cirrhosis and warrant further investigation in larger, longitudinal studies.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2678639"},"PeriodicalIF":6.7,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13218307/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148138446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between the oral microbiome and clinical indicators of resectable non-small cell lung cancer (NSCLC).","authors":"Vanessa DeClercq, Rowan E Murphy, Nick Zinck, Morgan G I Langille, Alison Wallace","doi":"10.1080/20002297.2026.2675065","DOIUrl":"10.1080/20002297.2026.2675065","url":null,"abstract":"<p><strong>Background: </strong>Oral microbial implications for lung cancer outcomes and association with clinical markers are largely unknown.</p><p><strong>Objective: </strong>This work aimed to characterize the oral microbiome in relation to clinical indicators and identify oral microbial biomarkers associated with lung cancer outcomes in patients with non-small cell lung cancer (NSCLC).</p><p><strong>Design: </strong>This is an observational prospective study of saliva samples from patients undergoing curative surgery for NSCLC. Taxonomic and composition profiles were generated using full-length 16S rRNA gene sequencing (n=64). Differences in bacterial diversity and composition with cancer stage, histological subtype, overall survival (OS), and event-free survival (EFS) were examined.</p><p><strong>Results: </strong>Most alpha diversity measures were lower with stage III cancer compared to stage I. All other clinical features were not statistically associated with alpha diversity (P>0.05). The relative abundance of Haemophilus and Solobacterium was detected as significantly differentially abundant in participants with stage III NSCLC by at least 2 differentially abundant tools and an FDR-corrected p-value<0.1.</p><p><strong>Conclusion: </strong>This study shows a significant association between cancer stage with oral bacterial diversity and the relative abundance of specific genera in relation to OS in participants with resectable NSCLC. This study is limited by sample size and needs to be confirmed in larger studies.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2675065"},"PeriodicalIF":6.7,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13202686/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148042583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of extracellular vesicles in intercellular communication within the oral cavity.","authors":"Abdelhabib Semlali, Mohammed Al-Zharani, Jassem Al-Ansari","doi":"10.1080/20002297.2026.2673760","DOIUrl":"10.1080/20002297.2026.2673760","url":null,"abstract":"<p><strong>Background: </strong>Extracellular vesicles (EVs), including exosomes, microvesicles, and apoptotic bodies, are nanoscale membrane-bound structures that mediate intercellular communication through the transfer of proteins, lipids, and nucleic acids. In the oral cavity, EVs are present in saliva and gingival crevicular fluid, where they contribute to immune regulation, epithelial barrier integrity, and host-microbiome interactions.</p><p><strong>Objective: </strong>This review aimed to critically summarize current knowledge regarding EV biogenesis, molecular composition, cellular and microbial sources, and their roles in oral homeostasis and disease pathogenesis.</p><p><strong>Results: </strong>Current evidence demonstrates that EVs derived from host cells and oral microorganisms actively regulate inflammatory responses, biofilm dynamics, tissue regeneration, and microbial colonization. Dysregulation of EV-mediated communication has been implicated in the development and progression of periodontal disease, oral candidiasis, and oral squamous cell carcinoma (OSCC). In addition, salivary EVs have emerged as promising non-invasive biomarkers and potential therapeutic tools in oral medicine. However, important methodological limitations remain, particularly regarding EV isolation, characterization, and standardization.</p><p><strong>Conclusions: </strong>EVs represent central mediators of communication within the oral microenvironment and play dual roles in maintaining oral homeostasis and promoting disease progression. Further standardized and clinically relevant studies are required to better define EV biology and facilitate the translation of EV-based diagnostic and therapeutic strategies into clinical oral medicine.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2673760"},"PeriodicalIF":6.7,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13202678/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148042536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}