Journal of Oral Microbiology最新文献_第9页

Inhibition of biofilm formation and virulence factors of cariogenic oral pathogen Streptococcus mutans by natural flavonoid phloretin. 天然黄酮根皮素对口腔致病菌变形链球菌生物膜形成及毒力因子的抑制作用。

IF 4.5 2区医学

Journal of Oral Microbiology Pub Date : 2023-01-01 DOI: 10.1080/20002297.2023.2230711

Lucille Rudin, Michael M Bornstein, Viktoriya Shyp

{"title":"Inhibition of biofilm formation and virulence factors of cariogenic oral pathogen Streptococcus mutans by natural flavonoid phloretin.","authors":"Lucille Rudin, Michael M Bornstein, Viktoriya Shyp","doi":"10.1080/20002297.2023.2230711","DOIUrl":"https://doi.org/10.1080/20002297.2023.2230711","url":null,"abstract":"Objectives: To evaluate the effect and mechanism of action of the flavonoid phloretin on the growth and sucrose-dependent biofilm formation of Streptococcus mutans.Methods: Minimum inhibitory concentration, viability, and biofilm susceptibility assays were conducted to assess antimicrobial and antibiofilm effect of phloretin. Biofilm composition and structure were analysed with scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). Water-soluble (WSG) and water-insoluble glucan (WIG) were determined using anthrone method. Lactic acid measurements and acid tolerance assay were performed to assess acidogenicity and aciduricity. Reverse transcription quantitative PCR (RT-qPCR) was used to measure the expression of virulence genes essential for surface attachment, biofilm formation, and quorum sensing.Results: Phloretin inhibited S. mutans growth and viability in a dose-dependent manner. Furthermore, it reduced gtfB and gtfC gene expression, correlating with the reduction of extracellular polysaccharides (EPS)/bacteria and WIG/WSG ratio. Inhibition of comED and luxS gene expression, involved in stress tolerance, was associated with compromised acidogenicity and aciduricity of S. mutans.Conclusions: Phloretin exhibits antibacterial properties against S. mutans, modulates acid production and tolerance, and reduces biofilm formation.Clinical significance: Phloretin is a promising natural compound with pronounced inhibitory effect on key virulence factors of the cariogenic pathogen, S. mutans.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"15 1","pages":"2230711"},"PeriodicalIF":4.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/8d/3a/ZJOM_15_2230711.PMC10321187.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10195137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ex vivo evaluation of antibiotic sensitivity in samples from endodontic infections. 牙髓感染样品中抗生素敏感性的体外评估。

IF 4.5 2区医学

Journal of Oral Microbiology Pub Date : 2023-01-01 DOI: 10.1080/20002297.2022.2160536

Álvaro Villanueva-Castellote, Carmen Llena Puy, Miguel Cerda-Diéguez, Álex Mira, María D Ferrer

{"title":"Ex vivo evaluation of antibiotic sensitivity in samples from endodontic infections.","authors":"Álvaro Villanueva-Castellote, Carmen Llena Puy, Miguel Cerda-Diéguez, Álex Mira, María D Ferrer","doi":"10.1080/20002297.2022.2160536","DOIUrl":"https://doi.org/10.1080/20002297.2022.2160536","url":null,"abstract":"Objective: To develop an in vitro model for real-time monitoring of endodontic biofilm growth and evaluate the ex vivo effect of antibiotics on biofilm growth.Material and methods: Root canal samples were taken from 40 patients and inoculated into 96-well plates in a system that measures biofilm growth through electrical impedance. Biofilm bacterial composition at the genus and species level was analyzed by Illumina sequencing. ANCOM-BC corrected data were used to compare bacterial composition after antibiotic treatment through compositional analysis, and to compare microbiological with clinical data.Results: The stationary phase was reached at 8 hours. The biofilm formed had a similar bacterial composition to the inoculum, and Enterococcus faecalis was virtually absent from the samples. The bacterial composition and the effect of antibiotics were sample-dependent. Metronidazole was the antibiotic that most inhibited biofilm formation and azithromycin the one that inhibited it in the highest percentage of cases. The antibiotic effect could not be related to the biofilm original bacterial composition.Conclusions: The impedance system allowed real-time monitoring of endodontic biofilm formation, and we propose it as a model for ex vivo evaluation of the whole biofilm susceptibility to antimicrobials, as opposed to evaluating antibiotic sensitivity of specific bacterial isolates.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"15 1","pages":"2160536"},"PeriodicalIF":4.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9793940/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10491631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A tightly controlled gene induction system that contributes to the study of lethal gene function in Streptococcus mutans. 一个严格控制的基因诱导系统，有助于研究变异链球菌的致死基因功能。

IF 4.5 2区医学

Journal of Oral Microbiology Pub Date : 2023-01-01 DOI: 10.1080/20002297.2023.2253675

Yongliang Li, Guanwen Li, Xuliang Deng

{"title":"A tightly controlled gene induction system that contributes to the study of lethal gene function in Streptococcus mutans.","authors":"Yongliang Li, Guanwen Li, Xuliang Deng","doi":"10.1080/20002297.2023.2253675","DOIUrl":"https://doi.org/10.1080/20002297.2023.2253675","url":null,"abstract":"Effective control of gene expression is crucial for understanding gene function in both eukaryotic and prokaryotic cells. While several inducible gene expression systems have been reported in Streptococcus mutans, a conditional pathogen that causes dental caries, the significant non-inducible basal expression in these systems seriously limits their utility, especially when studying lethal gene functions and molecular mechanisms. We introduce a tightly controlled xylose-inducible gene expression system, TC-Xyl, for Streptococcus mutans. Western blot results and fluorescence microscopy analysis indicate that TC-Xyl exhibits an extremely low non-inducible basal expression level and a sufficiently high expression level post-induction. Further, by constructing a mutation in which the only source FtsZ is under the control of TC-Xyl, we preliminarily explored the function of the ftsz gene. We found that FtsZ depletion is lethal to Streptococcus mutans, resulting in abnormal round cell shape and mini cell formation, suggesting FtsZ's role in maintaining cell shape stability.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"15 1","pages":"2253675"},"PeriodicalIF":4.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10486305/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10218646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trans-cinnamaldehyde loaded chitosan based nanocapsules display antibacterial and antibiofilm effects against cavity-causing Streptococcus mutans. 负载反式肉桂醛的壳聚糖纳米胶囊对引起蛀牙的变形链球菌具有抗菌和抗生物膜作用。

IF 4.5 2区医学

Journal of Oral Microbiology Pub Date : 2023-01-01 DOI: 10.1080/20002297.2023.2243067

Ran Mu, Hanyi Zhang, Zhiyuan Zhang, Xinyue Li, Jiaxuan Ji, Xinyue Wang, Yu Gu, Xiaofei Qin

{"title":"Trans-cinnamaldehyde loaded chitosan based nanocapsules display antibacterial and antibiofilm effects against cavity-causing Streptococcus mutans.","authors":"Ran Mu, Hanyi Zhang, Zhiyuan Zhang, Xinyue Li, Jiaxuan Ji, Xinyue Wang, Yu Gu, Xiaofei Qin","doi":"10.1080/20002297.2023.2243067","DOIUrl":"https://doi.org/10.1080/20002297.2023.2243067","url":null,"abstract":"Background: Dental caries is a multifactorial disease, and the bacteria such as Streptococcus mutans (S. mutans) is one of the risk factors. The poor effect of existing anti-bacterial is mainly related to drug resistance, the short time of drug action, and biofilm formation.Methods: To address this concern, we report here on the cinnamaldehyde (CA) loaded chitosan (CS) nanocapsules (CA@CS NC) sustained release CA for antibacterial treatment. The size, ζ-potential, and morphology were characterized. The antibacterial activities in vitro were studied by growth curve assay, pH drop assay, biofilm assay, and qRT-PCR In addition, cytotoxicity assay, organ index, body weight, and histopathology results were analyzed to evaluate the safety and biocompatibility in a rat model.Results: CA@CS NC can adsorb the bacterial membrane due to electronic interaction, releasing CA slowly for a long time. At the same time, it has reliable antibacterial activity against S. mutans and downregulated the expression levels of QS, virulence, biofilm, and adhesion genes. In addition, it greatly reduced the cytotoxicity of CA and significantly inhibited dental caries in rats without obvious toxicity.Conclusion: Our results showed that CA@CS NC had antibacterial and antibiofilm effects on S. mutans and inhibit dental caries. Besides, it showed stronger efficacy and less toxicity, and was able to adsorb bacteria releasing CA slowly, providing a new nanomaterial solution for the treatment of dental caries.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"15 1","pages":"2243067"},"PeriodicalIF":4.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e4/0e/ZJOM_15_2243067.PMC10402844.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10565049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dental caries and their microbiomes in children: what do we do now? 儿童龋齿及其微生物群:我们现在该怎么办?

IF 4.5 2区医学

Journal of Oral Microbiology Pub Date : 2023-01-01 DOI: 10.1080/20002297.2023.2198433

Apoena Aguiar Ribeiro, Bruce J Paster

{"title":"Dental caries and their microbiomes in children: what do we do now?","authors":"Apoena Aguiar Ribeiro, Bruce J Paster","doi":"10.1080/20002297.2023.2198433","DOIUrl":"https://doi.org/10.1080/20002297.2023.2198433","url":null,"abstract":"The oral cavity is an unique ecosystem formed by different structures, tissues, and a complex microbial community formed by hundreds of different species of bacteria, fungi, viruses, phages, and the candidate phyla radiation (CPR) group, all living in symbiosis with healthy individuals. In an opposite state, dental caries is a biofilm-mediated dysbiosis that involves changes in the core microbiome composition and function, which leads to the demineralization of tooth tissues due to the fermentation of dietary carbohydrates, producing acid by select oral bacteria. The cariogenic biofilm is typically characterized by bacterial species with the ability of adhering to the saliva-coated tooth surface, production of exopolysaccharides-rich matrix (which will limit the diffusion of acidic products of carbohydrate fermentation), and the ability of surviving in this acidic environment. Besides years of research and dental treatment, dental caries remains the most common chronic disease in children worldwide. This article aims to bring an insightful discussion about important questions that remain unanswered in the Cariology and Oral Microbiology fields, to move Science forward, characterize the interrelationships of these communities, and understand mechanistic functions between microorganisms and the host, therefore leading to translatable knowledge that benefits the provision of care to our pediatric patients.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"15 1","pages":"2198433"},"PeriodicalIF":4.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/cb/f5/ZJOM_15_2198433.PMC10088930.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9359467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Association of nine pathobionts with periodontitis in four South American and European countries. 九种病原体与牙周炎在四个南美和欧洲国家的关联。

IF 4.5 2区医学

Journal of Oral Microbiology Pub Date : 2023-01-01 DOI: 10.1080/20002297.2023.2188630

Gerard Àlvarez, Alexandre Arredondo, Sergio Isabal, Wim Teughels, Isabelle Laleman, María José Contreras, Lorena Isbej, Enrique Huapaya, Gerardo Mendoza, Carolina Mor, José Nart, Vanessa Blanc, Rubén León

{"title":"Association of nine pathobionts with periodontitis in four South American and European countries.","authors":"Gerard Àlvarez, Alexandre Arredondo, Sergio Isabal, Wim Teughels, Isabelle Laleman, María José Contreras, Lorena Isbej, Enrique Huapaya, Gerardo Mendoza, Carolina Mor, José Nart, Vanessa Blanc, Rubén León","doi":"10.1080/20002297.2023.2188630","DOIUrl":"https://doi.org/10.1080/20002297.2023.2188630","url":null,"abstract":"Aim: Our aim was to compare the prevalence and load of nine pathobionts in subgingival samples of healthy individuals and periodontitis patients from four different countries.Methods: Five hundred and seven subgingival biofilm samples were collected from healthy subjects and periodontitis patients in Belgium, Chile, Peru and Spain. The prevalence and load of Eubacterium brachy, Filifactor alocis, Fretibacterium fastidiosum, Porphyromonas endodontalis, Porphyromonas gingivalis, Selenomonas sputigena, Treponema denticola, Tannerella forsythia and Treponema socranskii were measured by quantitative PCR.Results: The association with periodontitis of all species, except for T. socranskii, was confirmed in all countries but Peru, where only P. endodontalis, P. gingivalis and T. denticola were found to be significantly associated. Moreover, most species showed higher loads at greater CAL and PPD, but not where there was BOP. Through Principal Component Analysis, samples showed clearly different distributions by diagnosis, despite observing a smaller separation in Peruvian samples.Conclusions: Unlike prevalence, relative load was found to be a reliable variable to discriminate the association of the species with periodontitis. Based on this, F. alocis, P. endodontalis, P. gingivalis, T. denticola and T. forsythia may be biomarkers of disease in Belgium, Chile and Spain, due to their significantly higher abundance in periodontitis patients.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"15 1","pages":"2188630"},"PeriodicalIF":4.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/4d/83/ZJOM_15_2188630.PMC10026778.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9518649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Gram-negative quorum sensing signalling enhances biofilm formation and virulence traits in gram-positive pathogen Enterococcus faecalis. 革兰氏阴性群体感应信号增强革兰氏阳性病原体粪肠球菌的生物膜形成和毒力特性。

IF 4.5 2区医学

Journal of Oral Microbiology Pub Date : 2023-01-01 DOI: 10.1080/20002297.2023.2208901

Ana Parga, Daniel Manoil, Malin Brundin, Ana Otero, Georgios N Belibasakis

{"title":"Gram-negative quorum sensing signalling enhances biofilm formation and virulence traits in gram-positive pathogen Enterococcus faecalis.","authors":"Ana Parga, Daniel Manoil, Malin Brundin, Ana Otero, Georgios N Belibasakis","doi":"10.1080/20002297.2023.2208901","DOIUrl":"https://doi.org/10.1080/20002297.2023.2208901","url":null,"abstract":"Acyl-homoserine lactones (AHLs) are typical quorum-sensing molecules of gram-negative bacteria. Recent evidence suggests that AHLs may also affect gram-positives, although knowledge of these interactions remains scarce. Here, we assessed the effect of AHLs on biofilm formation and transcriptional regulations in the gram-positive Enterococcus faecalis. Five E. faecalis strains were investigated herein. Crystal violet was employed to quantify the biomass formed, and confocal microscopy in combination with SYTO9/PI allowed the visualisation of biofilms' structure. The differential expression of 10 genes involved in quorum-sensing, biofilm formation and stress responses was evaluated using reverse-transcription-qPCR. The AHL exposure significantly increased biofilm production in strain ATCC 29212 and two isolates from infected dental roots, UmID4 and UmID5. In strains ATCC 29212 and UmID7, AHLs up-regulated the quorum-sensing genes (fsrC, cylA), the adhesins ace, efaA and asa1, together with the glycosyltransferase epaQ. In strain UmID7, AHL exposure additionally up-regulated two membrane-stress response genes (σV, groEL) associated with increased stress-tolerance and virulence. Altogether, our results demonstrate that AHLs promote biofilm formation and up-regulate a transcriptional network involved in virulence and stress tolerance in several E. faecalis strains. These data provide yet-unreported insights into E. faecalis biofilm responses to AHLs, a family of molecules long-considered the monopole of gram-negative signalling.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"15 1","pages":"2208901"},"PeriodicalIF":4.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10177678/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9468663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Newly identified pathogens in periodontitis: evidence from an association and an elimination study. 新发现的牙周炎病原体:来自关联和消除研究的证据。

IF 4.5 2区医学

Journal of Oral Microbiology Pub Date : 2023-01-01 DOI: 10.1080/20002297.2023.2213111

Eduardo Lobão Veras, Nídia Castro Dos Santos, João Gabriel S Souza, Luciene C Figueiredo, Belen Retamal-Valdes, Valentim A R Barão, Jamil Shibli, Martinna Bertolini, Marcelo Faveri, Flavia Teles, Poliana Duarte, Magda Feres

{"title":"Newly identified pathogens in periodontitis: evidence from an association and an elimination study.","authors":"Eduardo Lobão Veras, Nídia Castro Dos Santos, João Gabriel S Souza, Luciene C Figueiredo, Belen Retamal-Valdes, Valentim A R Barão, Jamil Shibli, Martinna Bertolini, Marcelo Faveri, Flavia Teles, Poliana Duarte, Magda Feres","doi":"10.1080/20002297.2023.2213111","DOIUrl":"https://doi.org/10.1080/20002297.2023.2213111","url":null,"abstract":"We assessed the level of evidence for the presence of new periodontal pathogens by (i) comparing the occurrence of non-classical periodontal taxa between healthy vs. periodontitis patients (Association study); (ii) assessing the modifications in the prevalence and levels of these species after treatments (Elimination study). In the Association study, we compared the prevalence and levels of 39 novel bacterial species between periodontally healthy and periodontitis patients. In the Elimination study, we analyzed samples from periodontitis patients assigned to receive scaling and root planing alone or with metronidazole+ amoxicillin TID/ 14 days. Levels of 79 bacterial species (39 novel and 40 classic) were assessed at baseline, 3 and 12 months post-therapy. All samples were analyzed using Checkerboard DNA-DNA hybridization. Out of the 39 novel species evaluated, eight were categorized as having strong and four as having moderate association with periodontitis. Our findings suggest strong evidence supporting Lancefieldella rimae, Cronobacter sakazakii, Pluralibacter gergoviae, Enterococcus faecalis, Eubacterium limosum, Filifactor alocis, Haemophilus influenzae, and Staphylococcus warneri, and moderate evidence supporting Escherichia coli, Fusobacterium necrophorum, Spiroplasma ixodetis, and Staphylococcus aureus as periodontal pathogens. These findings contribute to a better understanding of the etiology of periodontitis and may guide future diagnostic and interventional studies.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"15 1","pages":"2213111"},"PeriodicalIF":4.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10228317/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10529739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Screening of heat-killed lactic acid bacteria based on inhibitory activity against oral bacteria and effects of oral administration of heat-killed Ligilactobacillus salivarius CP3365 on periodontal health in healthy participants: a double-blinded, randomized, placebo-controlled trial. 基于对口腔细菌抑制活性的热杀灭乳酸菌筛选，以及口服热杀灭唾液乳酸杆菌CP3365对健康参与者牙周健康的影响:一项双盲、随机、安慰剂对照试验。

IF 4.5 2区医学

Journal of Oral Microbiology Pub Date : 2023-01-01 DOI: 10.1080/20002297.2023.2250649

Shinji Sakata, Yukiko Sakamaki, Masahiro Yuki, Tsutomu Sugaya, Tatsuhiko Hirota

{"title":"Screening of heat-killed lactic acid bacteria based on inhibitory activity against oral bacteria and effects of oral administration of heat-killed Ligilactobacillus salivarius CP3365 on periodontal health in healthy participants: a double-blinded, randomized, placebo-controlled trial.","authors":"Shinji Sakata, Yukiko Sakamaki, Masahiro Yuki, Tsutomu Sugaya, Tatsuhiko Hirota","doi":"10.1080/20002297.2023.2250649","DOIUrl":"https://doi.org/10.1080/20002297.2023.2250649","url":null,"abstract":"ABSTRACT Objectives The aims of this study were to select heat-killed lactic acid bacteria (HKL) with antibiotic activity and investigate the efficacy of this bacteria in maintaining periodontal parameters in healthy participants. Materials and methods An in vitro evaluation was conducted to assess the inhibitory efficacy of lactic acid bacteria against Porphyromonas gingivalis and Fusobacterium nucleatum subsp. nucleatum. The effects of HKL administration on various parameters (plaque control record, bleeding on probing, and probing pocket depth) were assessed in a randomized, placebo-controlled trial. Participants in the test and placebo groups (n = 32) consumed oral tablets containing placebo or HKL daily for 8 weeks. Oral bacteria in supra-plaque and saliva were identified using 16S rRNA gene community profiling analysis. Results Heat-killed Ligilactobacillus salivarius CP3365 significantly (p < 0.05) decreased the viability of oral bacteria and was selected for clinical trials. Administration of HKL CP3365 significantly (p < 0.05) inhibited increases in each parameter. No changes in the relative abundance of P. gingivalis or F. nucleatum subsp. nucleatum were detected by HKL CP3365, but the relative abundance of oral bacteria (genera Porphyromonas, Fusobacterium, and Haemophilus) was significantly (p < 0.05) decreased. Conclusion HKL CP3365 effectively inhibited oral bacteria growth and was useful for maintaining periodontal health. Clinical Trial Registration [https://www.umin.ac.jp/ctr/index.htm], identifier [UMIN000045656].","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"15 1","pages":"2250649"},"PeriodicalIF":4.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10464545/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10188106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The interplay between oral microbiota, gut microbiota and systematic diseases. 口腔菌群、肠道菌群与系统性疾病的相互作用。

IF 4.5 2区医学

Journal of Oral Microbiology Pub Date : 2023-01-01 DOI: 10.1080/20002297.2023.2213112

Xiujun Tan, Yizhong Wang, Ting Gong

{"title":"The interplay between oral microbiota, gut microbiota and systematic diseases.","authors":"Xiujun Tan, Yizhong Wang, Ting Gong","doi":"10.1080/20002297.2023.2213112","DOIUrl":"https://doi.org/10.1080/20002297.2023.2213112","url":null,"abstract":"Over the past two decades, the importance of microbiota in health and disease has become evident. The human gut microbiota and oral microbiota are the largest and second-largest microbiome in the human body, respectively, and they are physically connected as the oral cavity is the beginning of the digestive system. Emerging and exciting evidence has shown complex and important connections between gut microbiota and oral microbiota. The interplay of the two microbiomes may contribute to the pathological processes of many diseases, including diabetes, rheumatoid arthritis, nonalcoholic fatty liver disease, inflammatory bowel disease, pancreatic cancer, colorectal cancer, and so on. In this review, we discuss possible routes and factors of oral microbiota to affect gut microbiota, and the contribution of this interplay between oral and gut microbiota to systemic diseases. Although most studies are association studies, recently, there have been increasing mechanistic investigations. This review aims to enhance the interest in the connection between oral and gut microbiota, and shows the tangible impact of this connection on human health.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"15 1","pages":"2213112"},"PeriodicalIF":4.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/1f/a4/ZJOM_15_2213112.PMC10187086.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10192809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4