Journal of Oral Microbiology最新文献_第9页

Sub-lethal concentrations of chlorhexidine inhibit Candida albicans growth by disrupting ROS and metal ion homeostasis 亚致死浓度的氯己定通过破坏活性氧和金属离子稳态来抑制白色念珠菌的生长

2区医学

Journal of Oral Microbiology Pub Date : 2023-11-09 DOI: 10.1080/20002297.2023.2278937

Qian Jiang, Yuchen Deng, Shuaihu Li, Deqin Yang, Li Tao

{"title":"Sub-lethal concentrations of chlorhexidine inhibit Candida albicans growth by disrupting ROS and metal ion homeostasis","authors":"Qian Jiang, Yuchen Deng, Shuaihu Li, Deqin Yang, Li Tao","doi":"10.1080/20002297.2023.2278937","DOIUrl":"https://doi.org/10.1080/20002297.2023.2278937","url":null,"abstract":"Candida albicans is a normal resident of the human oral cavity. It is also the most common fungal pathogen, causing various oral diseases, particularly in immunocompromised individuals. Chlorhexidine digluconate (CHG) is a broad-spectrum antimicrobial agent widely used in dental practice and has been recommended to treat oral candidiasis. However, its action mechanism against the fungal pathogen C. albicans remains poorly understood. The aim of the present study was to investigate the effect of CHG at sub-lethal concentrations against C. albicans. CHG inhibited the growth of C. albicans in a dose- and time-dependent manner. Cells treated with CHG exhibited altered membrane permeability, reduced metabolic activity, and enhanced metal ion and reactive oxygen species (ROS) accumulation. Copper-sensing transcription factor Mac1, iron-sensing transcription factors Sfu1 and Sef2, and copper transporter Ctr1 regulated intracellular metal ion and ROS homeostasis in response to CHG. Deletion of MAC1, SFU1, or SEF2 increased intracellular ROS production and cell susceptibility to CHG. This study revealed a novel mechanism by which CHG induced apoptosis of C. albicans cells through the disruption of metal ion and ROS homeostasis, which may help to identify new targets for fungal infections.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":" 42","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135291262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiomics analysis reveals the genetic and metabolic characteristics associated with the low prevalence of dental caries. 多组学分析揭示了与龋齿低患病率相关的遗传和代谢特征。

IF 3.7 2区医学

Journal of Oral Microbiology Pub Date : 2023-11-02 eCollection Date: 2023-01-01 DOI: 10.1080/20002297.2023.2277271

Jinshen Liu, Si-Ying Ye, Xin-Dong Xu, Qiulin Liu, Fei Ma, Xueting Yu, Yu-Hong Luo, Ling-Ling Chen, Xiaojuan Zeng

{"title":"Multiomics analysis reveals the genetic and metabolic characteristics associated with the low prevalence of dental caries.","authors":"Jinshen Liu, Si-Ying Ye, Xin-Dong Xu, Qiulin Liu, Fei Ma, Xueting Yu, Yu-Hong Luo, Ling-Ling Chen, Xiaojuan Zeng","doi":"10.1080/20002297.2023.2277271","DOIUrl":"10.1080/20002297.2023.2277271","url":null,"abstract":"Background: Despite poor oral hygiene, the Baiku Yao (BKY) ethnic group in China presents a low prevalence of dental caries, which may be related to genetic susceptibility. Due to strict intra-ethnic marriage rule, this ethnic has an advantage in studying the interaction between genetic factors and other regulatory factors related to dental caries.Methods: Peripheral blood from a caries-free adult male was used for whole genome sequencing, and the BKY assembled genome was compared to the Han Chinese genome. Oral saliva samples were collected from 51 subjects for metabolomic and metagenomic analysis. Multiomics data were integrated for combined analysis using bioinformatics approaches.Results: Comparative genomic analysis revealed the presence of structural variations in several genes associated with dental caries. Metabolomic and metagenomic sequencing demonstrated the caries-free group had significantly higher concentration of antimicrobials and higher abundance of core oral health-related microbiota. The functional analysis indicated that cationic antimicrobial peptide resistance and the lipopolysaccharide biosynthesis pathway were enriched in the caries-free group.Conclusions: Our study provided new insights into the specific regulatory mechanisms that contribute to the low prevalence of dental caries in the specific population and may provide new evidence for the genetic diagnosis and control of dental caries.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"15 1","pages":"2277271"},"PeriodicalIF":3.7,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10623897/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71482618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral microbiota study of the patients after hospitalisation for COVID-19, considering selected dental indices and antibiotic therapy using the next generation sequencing method (NGS). 新冠肺炎患者住院后的口腔微生物群研究，考虑选择的牙科指标和使用下一代测序方法（NGS）的抗生素治疗。

IF 4.5 2区医学

Journal of Oral Microbiology Pub Date : 2023-10-11 eCollection Date: 2023-01-01 DOI: 10.1080/20002297.2023.2264591

Barbara Brzychczy-Sroka, Katarzyna Talaga-Ćwiertnia, Agnieszka Sroka-Oleksiak, Artur Gurgul, Elżbieta Zarzecka-Francica, Wojciech Ostrowski, Janusz Kąkol, Joanna Zarzecka, Monika Brzychczy-Włoch

{"title":"Oral microbiota study of the patients after hospitalisation for COVID-19, considering selected dental indices and antibiotic therapy using the next generation sequencing method (NGS).","authors":"Barbara Brzychczy-Sroka, Katarzyna Talaga-Ćwiertnia, Agnieszka Sroka-Oleksiak, Artur Gurgul, Elżbieta Zarzecka-Francica, Wojciech Ostrowski, Janusz Kąkol, Joanna Zarzecka, Monika Brzychczy-Włoch","doi":"10.1080/20002297.2023.2264591","DOIUrl":"10.1080/20002297.2023.2264591","url":null,"abstract":"Background: Poor oral hygiene and the increased incidence and severity of periodontitis may exacerbate SARS-CoV-2 infection. The aim was to evaluate the oral microbiota of 60 participants divided into groups: COVID-19 convalescents who received antibiotics during hospitalization (I), COVID-19 convalescents without antibiotic therapy (II) and healthy individuals (III).Materials and methods: Dental examination was conducted, and oral health status was evaluated using selected dental indexes. Clinical samples (saliva, dorsal swabs, supragingival and subgingival plaque) were collected and used for metagenomic library to the next-generation sequencing (NGS) preparation.Results: Each of the clinical materials in particular groups of patients showed a statistically significant and quantitatively different bacterial composition. Patients from group I showed significantly worse oral health, reflected by higher average values of dental indexes and also a higher percentage of Veillonella, Tannerella, Capnocytophaga and Selenomonas genera in comparison to other groups. Additionally, a statistically significant decrease in the amount of Akkermansia type in both groups with COVID-19 was observed for all materials.Conclusions: The primary factor affecting the composition of oral microbiota was not the SARS-CoV-2 infection itself, but the use of antibiotic therapy. The increased percentage of pro-inflammatory pathogens observed in COVID-19 patients underscores the importance of preventing periodontal disease and improving oral hygiene in the future.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"15 1","pages":"2264591"},"PeriodicalIF":4.5,"publicationDate":"2023-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b3/cd/ZJOM_15_2264591.PMC10569355.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41236167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral microbiome and ischemic stroke risk among elderly Chinese women. 中国老年妇女的口腔微生物组与缺血性中风风险。

IF 4.5 2区医学

Journal of Oral Microbiology Pub Date : 2023-10-08 eCollection Date: 2023-01-01 DOI: 10.1080/20002297.2023.2266655

Cong Wang, Yaohua Yang, Qiuyin Cai, Yutang Gao, Hui Cai, Jie Wu, Wei Zheng, Jirong Long, Xiao-Ou Shu

{"title":"Oral microbiome and ischemic stroke risk among elderly Chinese women.","authors":"Cong Wang, Yaohua Yang, Qiuyin Cai, Yutang Gao, Hui Cai, Jie Wu, Wei Zheng, Jirong Long, Xiao-Ou Shu","doi":"10.1080/20002297.2023.2266655","DOIUrl":"10.1080/20002297.2023.2266655","url":null,"abstract":"Background: Stroke, a leading cause of disability worldwide, has been associated with periodontitis. However, whether stroke risk is related to oral microbiota remains unknown. This study aims to evaluate the associations between the oral microbiome and ischemic stroke risk.Methods: In a case-control study of 134 case-control pairs nested within a prospective cohort study, we examined pre-diagnostic oral microbiome in association with stroke risk via shotgun metagenomic sequencing. The microbial sub-community and functional profiling were performed using Latent Dirichlet Allocation and HUMAnN2. Associations of microbial diversity, sub-community structure, and individual microbial features with ischemic stroke risk were evaluated via conditional logistic regression.Results: Alpha and beta diversities differ significantly between cases and controls. One genus- and two species-level sub-communities were significantly associated with decreased ischemic stroke risk, with odds ratios (95% confidence intervals) of 0.52 (0.31-0.90), 0.51 (0.31-0.84), and 0.60 (0.36-0.99), respectively. These associations were potentially driven by the representative taxa in these sub-communities, i.e., genus Corynebacterium and Lautropia, and species Lautropia mirabilis and Neisseria elongate (p < 0.05). Additionally, 55 taxa, 1,237 gene families, and 90 metabolic pathways were associated with ischemic stroke risk at p < 0.05.Conclusion: Our study highlights the role of oral microbiota in the etiology of ischemic stroke and calls for further research.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"15 1","pages":"2266655"},"PeriodicalIF":4.5,"publicationDate":"2023-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f1/40/ZJOM_15_2266655.PMC10563620.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41203528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alterations of oral microbiota are associated with the development and severity of acute pancreatitis. 口腔微生物群的改变与急性胰腺炎的发展和严重程度有关。

IF 4.5 2区医学

Journal of Oral Microbiology Pub Date : 2023-10-05 eCollection Date: 2023-01-01 DOI: 10.1080/20002297.2023.2264619

Yiting Liu, Hang Liu, Yuping Rong, Qiao Shi, Qiang Yang, Hanjun Li, Zhengle Zhang, Jing Tao

{"title":"Alterations of oral microbiota are associated with the development and severity of acute pancreatitis.","authors":"Yiting Liu, Hang Liu, Yuping Rong, Qiao Shi, Qiang Yang, Hanjun Li, Zhengle Zhang, Jing Tao","doi":"10.1080/20002297.2023.2264619","DOIUrl":"10.1080/20002297.2023.2264619","url":null,"abstract":"Acute pancreatitis (AP) is a common abdomen clinical emergency. Most APs have mild clinical symptoms and a good prognosis. However, about 20% of patients develop severe acute pancreatitis (SAP), increasing morbidity and mortality. The microbiome's impact on AP pathophysiology has received increasing attention. Hence, to explore changes in oral microbial composition in acute pancreatitis, we collected clinical information and oral saliva samples from 136 adult participants: 47 healthy controls, 43 acute mild AP (MAP), 29 moderate AP (MSAP), and 17 severe AP (SAP). Using 16S rRNA gene sequencing, 663,175 high-quality sequences were identified. The relative abundance and diversity of oral microorganisms in AP patients increased, with decreased beneficial bacteria such as Streptococcus, Neisseria, and Gemella, and increased Prevotella, Veillonella, Granulicatella, Actinomyces, and Peptostreptococcus in the AP group. Further changes in microbial composition occurred with increasing disease severity, including a decreased abundance of beneficial bacteria such as Neisseria, Haemophilus, and Gemella in MSAP and SAP compared to MAP. Moreover, the Lefse analysis showed that Prevotella, Peptostreptococcus, Actinomyces, and Porphyromonas were better microbial markers for AP. Therefore, oral microbiome changes could distinguish AP from healthy individuals and serve as an early novel predictor of disease severity in AP patients.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"15 1","pages":"2264619"},"PeriodicalIF":4.5,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10557549/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41148060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Smoking, tooth loss and oral hygiene practices have significant and site-specific impacts on the microbiome of oral mucosal surfaces: a cross-sectional study. 一项横断面研究显示，吸烟、牙齿脱落和口腔卫生习惯对口腔粘膜表面的微生物组有显著的、特定地点的影响。

IF 4.5 2区医学

Journal of Oral Microbiology Pub Date : 2023-10-02 eCollection Date: 2023-01-01 DOI: 10.1080/20002297.2023.2263971

Sheila Galvin, Sviatlana Anishchuk, Claire M Healy, Gary P Moran

{"title":"Smoking, tooth loss and oral hygiene practices have significant and site-specific impacts on the microbiome of oral mucosal surfaces: a cross-sectional study.","authors":"Sheila Galvin, Sviatlana Anishchuk, Claire M Healy, Gary P Moran","doi":"10.1080/20002297.2023.2263971","DOIUrl":"https://doi.org/10.1080/20002297.2023.2263971","url":null,"abstract":"We investigated bacterial colonisation patterns of healthy mucosa (buccal, tongue, palate and floor of mouth) in a cohort of adults in order to determine how smoking, tooth loss, plaque levels and oral hygiene practices impacted on mucosal colonisation. A total of 322 swabs were recovered from 256 participants, of whom 46% were current smokers. We analysed colonization by sequencing the V1-V3 regions of the 16S rRNA gene. Palate and tongue microbiomes generally exhibited greater biodiversity than buccal and floor of mouth. Although Neisseria, Lautropia and Haemophilus spp. showed reduced abundance in smokers, buccal mucosa specifically showed a significant increase in Prevotella spp., whereas tongue and floor of mouth tended towards increased abundance of Streptococcus spp. Unexpectedly, tooth brushing frequency had a greater impact on mucosal community structure than plaque levels. Tooth loss was associated with significant reductions in mucosal biodiversity and had site-specific impacts, with buccal communities showing increased abundance of periodontitis-associated species and Rothia mucilaginosa, whereas tongue communities exhibited increased abundance of several streptococcal OTUs and reduced abundance of Haemophilus spp. This study highlights the complex relationship between mucosal colonisation and host factors, highlighting the need for careful consideration of these factors in mucosal microbiome studies.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"15 1","pages":"2263971"},"PeriodicalIF":4.5,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10547447/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41117651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Site-specialization of human oral Gemella species. 人类口腔 Gemella 菌种的部位特化。

IF 3.7 2区医学

Journal of Oral Microbiology Pub Date : 2023-06-22 eCollection Date: 2023-01-01 DOI: 10.1080/20002297.2023.2225261

Julian Torres-Morales, Jessica L Mark Welch, Floyd E Dewhirst, Gary G Borisy

{"title":"Site-specialization of human oral Gemella species.","authors":"Julian Torres-Morales, Jessica L Mark Welch, Floyd E Dewhirst, Gary G Borisy","doi":"10.1080/20002297.2023.2225261","DOIUrl":"10.1080/20002297.2023.2225261","url":null,"abstract":"Gemella species are core members of the human oral microbiome in healthy subjects and are regarded as commensals, although they can cause opportunistic infections. Our objective was to evaluate the site-specialization of Gemella species among various habitats within the mouth by combining pangenomics and metagenomics. With pangenomics, we identified genome relationships and categorized genes as core and accessory to each species. With metagenomics, we identified the primary oral habitat of individual genomes. Our results establish that the genomes of three species, G. haemolysans, G. sanguinis and G. morbillorum, are abundant and prevalent in human mouths at different oral sites: G. haemolysans on buccal mucosa and keratinized gingiva; G. sanguinis on tongue dorsum, throat, and tonsils; and G. morbillorum in dental plaque. The gene-level basis of site-specificity was investigated by identifying genes that were core to Gemella genomes at a specific oral site but absent from other Gemella genomes. The riboflavin biosynthesis pathway was present in G. haemolysans genomes associated with buccal mucosa but absent from the rest of the genomes. Overall, metapangenomics show that Gemella species have clear ecological preferences in the oral cavity of healthy humans and provides an approach to identifying gene-level drivers of site specificity.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"15 1","pages":"2225261"},"PeriodicalIF":3.7,"publicationDate":"2023-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/23/ff/ZJOM_15_2225261.PMC10288933.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10530228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fusobacterium nucleatum dissemination by neutrophils. 核分枝杆菌通过中性粒细胞传播。

IF 3.7 2区医学

Journal of Oral Microbiology Pub Date : 2023-06-04 eCollection Date: 2023-01-01 DOI: 10.1080/20002297.2023.2217067

Felix Ellett, Nazli I Kacamak, Carla R Alvarez, Eduardo H S Oliveira, Hatice Hasturk, Bruce J Paster, Alpdogan Kantarci, Daniel Irimia

{"title":"Fusobacterium nucleatum dissemination by neutrophils.","authors":"Felix Ellett, Nazli I Kacamak, Carla R Alvarez, Eduardo H S Oliveira, Hatice Hasturk, Bruce J Paster, Alpdogan Kantarci, Daniel Irimia","doi":"10.1080/20002297.2023.2217067","DOIUrl":"10.1080/20002297.2023.2217067","url":null,"abstract":"Recent studies uncovered that Fusobacterium nucleatum (Fn), a common, opportunistic bacterium in the oral cavity, is associated with a growing number of systemic diseases, ranging from colon cancer to Alzheimer's disease. However, the pathological mechanisms responsible for this association are still poorly understood. Here, we leverage recent technological advances to study the interactions between Fn and neutrophils. We show that Fn survives within human neutrophils after phagocytosis. Using in vitro microfluidic devices, we determine that human neutrophils can protect and transport Fn over large distances. Moreover, we validate these observations in vivo by showing that neutrophils disseminate Fn using a zebrafish model. Our data support the emerging hypothesis that bacterial dissemination by neutrophils is a mechanistic link between oral and systemic diseases. Furthermore, our results may ultimately lead to therapeutic approaches that target specific host-bacteria interactions, including the dissemination process.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"15 1","pages":"2217067"},"PeriodicalIF":3.7,"publicationDate":"2023-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f7/7c/ZJOM_15_2217067.PMC10240972.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10174574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid specific detection of oral bacteria using Cas13-based SHERLOCK. 利用基于 Cas13 的 SHERLOCK 快速特异性检测口腔细菌。

IF 3.7 2区医学

Journal of Oral Microbiology Pub Date : 2023-05-11 eCollection Date: 2023-01-01 DOI: 10.1080/20002297.2023.2207336

Jett Liu, Camden Carmichael, Hatice Hasturk, Wenyuan Shi, Batbileg Bor

{"title":"Rapid specific detection of oral bacteria using Cas13-based SHERLOCK.","authors":"Jett Liu, Camden Carmichael, Hatice Hasturk, Wenyuan Shi, Batbileg Bor","doi":"10.1080/20002297.2023.2207336","DOIUrl":"10.1080/20002297.2023.2207336","url":null,"abstract":"Decades of ongoing research has established that oral microbial communities play a role in oral diseases such as periodontitis and caries. Yet the detection of oral bacteria and the profiling of oral polymicrobial communities currently rely on methods that are costly, slow, and technically complex, such as qPCR or next-generation sequencing. For the widescale screening of oral microorganisms suitable for point-of-care settings, there exists the need for a low-cost, rapid detection technique. Here, we tailored the novel CRISPR-Cas-based assay SHERLOCK for the species-specific detection of oral bacteria. We developed a computational pipeline capable of generating constructs suitable for SHERLOCK and experimentally validated the detection of seven oral bacteria. We achieved detection within the single-molecule range that remained specific in the presence of off-target DNA found within saliva. Further, we adapted the assay for detecting target sequences directly from unprocessed saliva samples. The results of our detection, when tested on 30 healthy human saliva samples, fully aligned with 16S rRNA sequencing. Looking forward, this method of detecting oral bacteria is highly scalable and can be easily optimized for implementation at point-of-care settings.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"15 1","pages":"2207336"},"PeriodicalIF":3.7,"publicationDate":"2023-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10177689/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10174031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current concepts in the pathogenesis of periodontitis: from symbiosis to dysbiosis. 牙周炎发病机制的当前概念：从共生到菌群失调。

IF 3.7 2区医学

Journal of Oral Microbiology Pub Date : 2023-04-02 eCollection Date: 2023-01-01 DOI: 10.1080/20002297.2023.2197779

Ali A Abdulkareem, Firas B Al-Taweel, Ali J B Al-Sharqi, Sarhang S Gul, Aram Sha, Iain L C Chapple

{"title":"Current concepts in the pathogenesis of periodontitis: from symbiosis to dysbiosis.","authors":"Ali A Abdulkareem, Firas B Al-Taweel, Ali J B Al-Sharqi, Sarhang S Gul, Aram Sha, Iain L C Chapple","doi":"10.1080/20002297.2023.2197779","DOIUrl":"10.1080/20002297.2023.2197779","url":null,"abstract":"The primary etiological agent for the initiation and progression of periodontal disease is the dental plaque biofilm which is an organized aggregation of microorganisms residing within a complex intercellular matrix. The non-specific plaque hypothesis was the first attempt to explain the role of the dental biofilm in the pathogenesis of periodontal diseases. However, the introduction of sophisticated diagnostic and laboratory assays has led to the realisation that the development of periodontitis requires more than a mere increase in the biomass of dental plaque. Indeed, multispecies biofilms exhibit complex interactions between the bacteria and the host. In addition, not all resident microorganisms within the biofilm are pathogenic, since beneficial bacteria exist that serve to maintain a symbiotic relationship between the plaque microbiome and the host's immune-inflammatory response, preventing the emergence of pathogenic microorganisms and the development of dysbiosis. This review aims to highlight the development and structure of the dental plaque biofilm and to explore current literature on the transition from a healthy (symbiotic) to a diseased (dysbiotic) biofilm in periodontitis and the associated immune-inflammatory responses that drive periodontal tissue destruction and form mechanistic pathways that impact other systemic non-communicable diseases.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"15 1","pages":"2197779"},"PeriodicalIF":3.7,"publicationDate":"2023-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10071981/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9324578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0