Runze Liu, Yi Luo, Pei Liu, Shuo Xiao, Wei Fan, Bing Fan
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引用次数: 0
Abstract
Objective: To investigate the role of mannose phosphotransferase system (Man-PTS) EII in the alkaline resistance of Enterococcus faecalis (E. faecalis).
Methods: The Man-PTS EIID (mptD) deletion (ΔmptD) and overexpressing (+mptD) strains of E. faecalis were constructed. The contribution of the Man-PTS EII to alkaline resistance was investigated by assessing growth and biofilm formation of E. faecalis at pH 7 and 10, as well as changes in intracellular ATP levels, pH, potassium ion concentration, membrane potential and membrane permeability. The effects of mptD on the expression of other Man-PTS EII components and membrane transport-related genes were evaluated by RT-qPCR at pH 10. In addition, six clinical strains were isolated. Three strains of E. faecalis with high to low alkaline resistance were screened, and RNA sequencing was performed to further explore the role of Man-PTS EII in the alkaline resistance.
Results: mptD enhanced the alkaline resistance of E. faecalis by maintaining pH homeostasis in an alkaline environment. Overexpression of mptD induced membrane hyperpolarization, reduced membrane permeability, decreased intracellular K+ levels, lowered cytoplasmic pH, and elevated ATP production, while the loss of mptD reversed these effects (p < 0.05). mptD coordinated the expression of Man-PTS EII components and upregulated genes encoding membrane transporter components (p < 0.01). All Man-PTS EII components were positively related to the degree of alkaline resistance of E. faecalis (p < 0.05).
Conclusion: Man-PTS EII is a key factor in the interaction between metabolism and ion membrane transport during the alkaline resistance process of E. faecalis. This study may provide new insights for understanding the alkaline resistance of E. faecalis.
期刊介绍：
As the first Open Access journal in its field, the Journal of Oral Microbiology aims to be an influential source of knowledge on the aetiological agents behind oral infectious diseases. The journal is an international forum for original research on all aspects of ''oral health''. Articles which seek to understand ''oral health'' through exploration of the pathogenesis, virulence, host-parasite interactions, and immunology of oral infections are of particular interest. However, the journal also welcomes work that addresses the global agenda of oral infectious diseases and articles that present new strategies for treatment and prevention or improvements to existing strategies.
Topics: ''oral health'', microbiome, genomics, host-pathogen interactions, oral infections, aetiologic agents, pathogenesis, molecular microbiology systemic diseases, ecology/environmental microbiology, treatment, diagnostics, epidemiology, basic oral microbiology, and taxonomy/systematics.
Article types: original articles, notes, review articles, mini-reviews and commentaries
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