Journal of Oral Microbiology最新文献

{"title":"Correlation between oral microbiota and dry socket at different time periods on tooth extraction.","authors":"Yujia Wu, Hujie Lyu, Xuliang Deng, Yong-Xin Liu, Ying He, Mingming Xu","doi":"10.1080/20002297.2025.2485210","DOIUrl":"https://doi.org/10.1080/20002297.2025.2485210","url":null,"abstract":"<p><strong>Background: </strong>Dry socket is a common post-extraction complication, characterized by the exposure of bone surfaces to the oral environment, leading to severe pain and potential infection. This study investigates the relationship between oral microbial composition and dry socket incidence in tooth extraction patients.</p><p><strong>Methods: </strong>From 87 patients (56 normal healing, 31 dry socket), 321 microbial samples were collected at pre-, med-, and post-extraction stages from saliva and the extraction sites, and all information was documented. All samples underwent 16S rDNA sequencing and amplicon analysis.</p><p><strong>Results: </strong>Dry socket patients exhibited distinct oral microbial diversity and composition. <i>Prevotella</i>, <i>Fusobacterium,</i> and <i>Haemophilus</i> strongly associated with the occurrence of dry socket. The microbial profiles in saliva revealed clearer temporal changes and healing/dry socket distinctions. The microbial network in the saliva of patients with dry socket exhibited key node/edge differences between med/post stages. Random forest analysis using pre-extraction saliva microbes to predict post-extraction symptoms, achieving a 75% accuracy rate in identifying the healthy group.</p><p><strong>Conclusion: </strong><i>Haemophilus</i> and <i>Fusobacterium</i> were key microbes in dry socket development and prediction. Functional changes caused by alterations in microbial composition and structure might have been the reason for the different symptoms observed after tooth extraction.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2485210"},"PeriodicalIF":3.7,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11980198/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144009594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of MreCD in <i>Streptococcus mutans</i>.","authors":"Victor Chan, Tessa Holcomb, Justin R Kaspar, Robert C Shields","doi":"10.1080/20002297.2025.2487643","DOIUrl":"https://doi.org/10.1080/20002297.2025.2487643","url":null,"abstract":"<p><strong>Background: </strong>Activities that control cell shape and division are critical for the survival of bacteria. However, little is known about the circuitry controlling these processes in the dental caries pathogen <i>Streptococcus mutans</i>.</p><p><strong>Methodology: </strong>We designed experiments to characterize two genes, <i>mreC</i> and <i>mreD</i>, in <i>S. mutans.</i> Assays included cell morphology imaging, protein interaction analysis, transcriptomics, proteomics, and biofilm studies to generate a comprehensive understanding of the role of MreCD in <i>S. mutans</i>.</p><p><strong>Results: </strong>Consistent with <i>mreCD</i> participating in cell elongation, cells lacking these genes were found to be rounder than wild-type cells. Using bacterial two-hybrid assays, interactions between MreCD and several other proteins implicated in cell elongation were observed. Further characterization, using proteomics, revealed that the surface-associated proteome is different in mutants lacking <i>mreCD</i>. Consistent with these changes we observed altered sucrose-mediated biofilm architecture. Loss of <i>mreCD</i> also had a noticeable impact on bacteriocin gene expression, which could account in part for the observation that <i>mreCD</i> mutants had a diminished capacity to compete with commensal streptococci.</p><p><strong>Conclusion: </strong>Our results provide evidence that cell elongation proteins are required for normal <i>S. mutans</i> physiology and establish a foundation for additional examination of these and related proteins in this organism.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2487643"},"PeriodicalIF":3.7,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11980242/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Porphyromonas gingivalis</i>-OMVs promote the epithelial-mesenchymal transition of oral squamous cell carcinoma by inhibiting ferroptosis through the NF-κB pathway.","authors":"Xinyue Liao, Hang Si, Yongxian Lai, Xiaoyan Zhang, Yun Feng, Tiejun Zhou, Yan Feng, Li Yu","doi":"10.1080/20002297.2025.2482924","DOIUrl":"https://doi.org/10.1080/20002297.2025.2482924","url":null,"abstract":"<p><strong>Background: </strong>Recent studies reported the role of <i>Porphyromonas gingivalis</i> (<i>P. g</i>) in promoting oral squamous cell carcinoma (OSCC) progression. However, the molecular mechanism remains unclear.</p><p><strong>Materials and methods: </strong><i>P. g</i>-OMVs were isolated using ultracentrifugation method and characterized by transmission electron microscopy (TEM) and nanoparticle tracking analysis (NTA). CCK-8, migration, invasion, Quantitative real-time Polymerase Chain Reaction (qRT-PCR) and immunocytochemistry assays were performed to evaluate the effect of <i>P. g</i>-OMVs on tumor cells' proliferation, migration, invasion, epithelial-mesenchymal transition (EMT), and ferroptosis in vitro. Western blot was performed to study the phosphorylation of transcription factor nuclear factor kappa B (NF-κB). In vivo, the effect of <i>P. g</i>-OMVs on the growth of OSCC was evaluated using a xenograft tumor model, followed by hematoxylin and eosin and immunohistochemistry staining.</p><p><strong>Results: </strong>TEM and NTA demonstrated that <i>P. g</i>-OMVs have a vesicular structure with a particle size of around 118 nm. Compared to the control group, <i>P. g</i>-OMVs significantly enhance the proliferation, migration, and invasion of tumor cells. In addition, <i>P.</i> <i>g</i>-OMVs promote the EMT of OSCC cells, which can be attenuated by ferroptosis activator erastin. Moreover, <i>P. g</i>-OMVs inhibit feroptosis of OSCC by activating NF-κB signaling. In vivo, <i>P. g</i>-OMVs significantly enhance tumor growth of OSCC. Inhibition of NF-κB could significnatly reduce the growth of OSCC, which can be further rescued using ferroptosis inhibitor Ferrostain-1.</p><p><strong>Conclusions: </strong><i>P. g</i>-OMVs promote OSCC progression by modulating the ferroptosis-related EMT through NF-κB signaling.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2482924"},"PeriodicalIF":3.7,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11980236/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143997239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High prevalence of antibiotic resistance of <i>Streptococcus</i> species in saliva from non-hospitalized adults - a pilot study.","authors":"Maria Nordholt Dollas, Martin Nilsson, Tove Larsen, Nikoline Nygaard, Claus Moser, Daniel Belstrøm","doi":"10.1080/20002297.2025.2486647","DOIUrl":"10.1080/20002297.2025.2486647","url":null,"abstract":"<p><strong>Background: </strong>Antibiotic resistance (AR) is a recognized threat to global human health. However, the prevalence of AR in healthy adults is not well described. The present observational pilot study aimed to uncover the potential of using saliva samples for screening for antibiotic resistance.</p><p><strong>Methodology: </strong>A laboratory protocol was developed for screening of AR in saliva samples, which was tested and validated using saliva samples collected from 100 study participants. The risk of AR was analyzed with descriptive statistics and evaluated using a risk-factor profile based on information on antibiotic usage within the last 12 months, education level and origin of birth.</p><p><strong>Results: </strong>AR was identified in 43 (48%) saliva samples, out of which 60,0% and 17,1% of resistant strains displayed resistance to clindamycin and penicillin, respectively. Streptococcus salivarius and Streptococcus parasanguinis were most often identified with AR (51,4% of all cases). The risk of AR was not associated with self-perceived oral or general health, antibiotic use within the latest 12 months or any demographic or socioeconomic parameters recorded. The risk-factor profile was observed in 44% in the AR group versus 30% in the non-AR group (<i>p</i> = 0.19).</p><p><strong>Conclusion: </strong>The present study showed that it is possible to perform non-invasive saliva-based screening for AR with a frequency of 48% of the samples, highlighting that saliva samples could be a valuable supplement to current surveillance methodologies for AR in the oral microbiota.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2486647"},"PeriodicalIF":3.7,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11966976/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intratumoral <i>Fusobacterium nucleatum</i> is associated with better cancer-specific survival in head and neck cancer patients.","authors":"José Guilherme Datorre, Mariana Bisarro Dos Reis, Bruna Pereira Sorroche, Gustavo Ramos Teixeira, Silveli Suzuki Hatano, Ana Carolina de Carvalho, Ricardo Ribeiro Gama, Lidia Maria Rebolho Batista Arantes, Rui Manuel Reis","doi":"10.1080/20002297.2025.2487644","DOIUrl":"10.1080/20002297.2025.2487644","url":null,"abstract":"<p><strong>Background: </strong>The oral microbiome, particularly <i>Fusobacterium nucleatum (Fn)</i>, has been implicated in head and neck cancers (HNC), influencing local immunity and Human Papillomavirus (HPV) status. Here, we evaluated the presence of <i>Fn</i> and its association with HPV infection, <i>TERT</i> promoter (<i>TERTp</i>) mutations, and patient outcomes.</p><p><strong>Materials and methods: </strong>We analyzed 94 formalin-fixed paraffin-embedded (FFPE) tumor tissues from HNC patients previously evaluated for <i>TERTp</i> mutations. <i>Fn</i> DNA was detected using droplet digital PCR (ddPCR), and HPV status was determined via p16 immunohistochemistry in pre-treatment samples. Associations between <i>Fn</i> presence, clinicopathological features, HPV, and TERTp mutation status were assessed.</p><p><strong>Results: </strong>Tumors primarily originated from the oropharynx (70.2%) and oral cavity (29.8%). Tobacco and alcohol use were reported in 87.2% and 79.8% of cases, respectively. <i>Fn</i> was present in 59.6% of cases, with higher prevalence in oropharyngeal (62.1%) than oral cavity (53.6%) tumors. No significant associations were found between <i>Fn</i> and clinicopathological features, TERTp, or HPV status. However, patients with <i>Fn</i> positivity showed significantly improved cancer-specific survival (61.5% vs. 39.1%, <i>p</i> = 0.013), similar to HPV-positive patients (72.7% vs. 42.7%, <i>p</i> = 0.014).</p><p><strong>Conclusion: </strong>The presence of <i>Fusobacterium nucleatum</i> in HNC correlates with longer survival, highlighting its potential as a prognostic marker.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2487644"},"PeriodicalIF":3.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11966973/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of increased <i>Porphyromonas gingivalis</i> peptidylarginine deiminase (PPAD) T2 variant allele on oral microbiota composition and severity of chronic periodontitis.","authors":"Marta Kaminska, Noemie A M Dudzinska, Tülay Yucel-Lindberg, Birgitta Söder, Aswathy Narayanan, Jan Potempa, Piotr M Mydel","doi":"10.1080/20002297.2025.2479903","DOIUrl":"10.1080/20002297.2025.2479903","url":null,"abstract":"<p><strong>Background: </strong><i>Porphyromonas gingivalis</i> (Pg) is a keystone pathogen in periodontitis, encoding a unique peptidyl arginine deiminase (PPAD) linked to protein citrullination, a process associated with rheumatoid arthritis (RA). Recently, we identified a super-active PPAD variant (T2) in <i>Pg</i> isolates. Here, we evaluated if the presence of the super-active T2 variant of PPAD affects the salivary microbiome, the severity of chronic periodontitis (CP), and subsequently CP's causative association with RA onset/progression.</p><p><strong>Patients/materials and methods: </strong>We examined 56 CP patients and 36 healthy volunteers. <i>Pg</i> and <i>Tannerella forsythia</i> counts were measured <i>via</i> RT-PCR, and PPAD variant was typed <i>via</i> PCR. 16S rRNA from salivary DNA sequencing characterized microbiota composition, while CP severity was assessed through bleeding on probing (BoP), clinical attachment loss (CAL), and pocket depth (PD) parameters.</p><p><strong>Results: </strong>CP patients exhibited higher <i>Pg</i> and <i>T.</i> <i>forsythia</i> counts, with 30.7% harbouring the PPAD-T2 variant, compared to only one healthy volunteer. Clinical CP parameters were unaffected by the PPAD variant. However, PPAD-T2 influenced oral microbiota composition, enriching certain genera.</p><p><strong>Conclusion: </strong>While the PPAD variant did not affect CP severity, it influenced oral microbiota composition. Further research is needed to understand citrullination's role in oral microbiota and chronic inflammatory disease development.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2479903"},"PeriodicalIF":3.7,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11926895/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation of periodontitis with hepatic and intestinal inflammation and glycemic control, and effects of bioconverted <i>Artemisia herba-alba</i> by <i>Lactiplantibacillus plantarum</i> SMFM2016-RK.","authors":"Yewon Lee, Yohan Yoon, Kyoung-Hee Choi","doi":"10.1080/20002297.2025.2473246","DOIUrl":"10.1080/20002297.2025.2473246","url":null,"abstract":"<p><p>Periodontitis has been linked to systemic inflammation, however research on its role in causing systemic diseases remains limited. Recent studies explore probiotics for microbiome modulation and enhancing natural compound bioavailability. This study investigated periodontitis-related systemic disease mechanisms, and evaluated the mitigation effects of bioconversion product using <i>Lactiplantibacillus plantarum</i> SMFM2016-RK and <i>Artemisia herba-alba</i> extracts. Four types of bioconverted milk [BM1 (<i>L. plantarum</i> SMFM2016-RK), BM2 (BM1 + <i>A. herba-alba</i> ethanol extract), BM3 (BM1 + <i>A. herba-alba</i> hot-water extract), and BM4 (BM1+ both <i>A. herba-alba</i> extracts)] were studied in a periodontitis-induced rat model. Rats were divided into six groups: normal control, skim milk with ligature, and four BM groups with ligature.   Periodontitis induction elevated trabecular resorption (0.325 ± 0.057 mm³) and histopathological symptoms. Serum ALT (55.6 ± 6.6 U/L), glucose (261.7 ± 64.3 mg/dL), insulin (1.90 ± 0.87 ng/mL), inflammation in the liver and colon, and gluconeogenesis-related enzyme expression increased. Periodontitis-induced rats showed gut dysbiosis, with decreased <i>Lactobacillaceae</i> level and increased <i>Oscillospiraceae</i> level. BM3 administration significantly reduced the serum glucose (190.9 ± 27.8 mg/dL), ALT (40.5 ± 5.0 U/L), inflammation, and gluconeogenesis-related enzymes, while increasing tight junction proteins expression and phylum Actinobacteria levels in the gut microbiome. The findings highlight the systemic impact of periodontitis on inflammation, glycemic control, and gut microbiome balance. BM3 effectively alleviated these effects suggesting therapeutic potential.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2473246"},"PeriodicalIF":3.7,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912291/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel approaches on root canal disinfection methods against <i>E. faecalis</i>.","authors":"Binhong Wu, Zhinuo Zhou, Xingwen Hong, Ziman Xu, Yuou Xu, Yushan He, Shu Chen","doi":"10.1080/20002297.2025.2475947","DOIUrl":"10.1080/20002297.2025.2475947","url":null,"abstract":"<p><strong>Background: </strong>Enterococcus faecalis (<i>E. faecalis</i>), the main pathogenic bacterium of root canal infection, can penetrate deep into the dentin tubule, form a biofilm, and resist host defense mechanisms, thereby increasing treatment complexity. Therefore, the key to the treatment of root canal infections is to completely kill the bacteria and prevent secondary infection. This review assesses advancements in traditional and novel disinfection methods targeting <i>E. faecalis</i> biofilm.</p><p><strong>Methods: </strong>By comparing the bactericidal mechanisms and effects of the individual and combined application of these methods, the scientific basis and clinical application potential of these methods as adjuvant or alternative treatments were evaluated and the scientific basis for the optimization of the root canal treatment strategy was provided.</p><p><strong>Results: </strong>Emerging strategies, including natural medicine, antibacterial photodynamic therapy, and cold atmospheric plasma, have shown promising antibacterial effects.</p><p><strong>Conclusion: </strong>These approaches have the potential to replace traditional disinfection methods, offering more effective solutions for clinical pulp treatment.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2475947"},"PeriodicalIF":3.7,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11892053/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Candida albicans</i> and NCAC species: acidogenic and fluoride-resistant oral inhabitants.","authors":"Haneen Raafat Fathi Mousa, Yuki Abiko, Jumpei Washio, Satoko Sato, Nobuhiro Takahashi","doi":"10.1080/20002297.2025.2473938","DOIUrl":"10.1080/20002297.2025.2473938","url":null,"abstract":"<p><strong>Objective: </strong>Although <i>Candida</i> species are thought to contribute to dental caries, their acid production under anaerobic conditions and susceptibility to fluoride have not been thoroughly studied. We therefore investigated the growth, acid production, and effect of fluoride on <i>Candida</i> species.</p><p><strong>Methods: </strong>Aerobic growth, acid production from glucose and its end-products under aerobic and anaerobic conditions, and enolase activity were measured in <i>C.</i> <i>albicans</i> and non-<i>Candida-albicans-Candida</i> (NCAC) species (<i>C.</i> <i>tropicalis</i>, <i>C.</i> <i>parapsilosis</i>, <i>C.</i> <i>maltosa</i>, and <i>C.</i> <i>glabrata</i>), and the effect of fluoride on these abilities was evaluated.</p><p><strong>Results: </strong>All <i>Candida</i> species produced acids under aerobic and anaerobic conditions, and acetate and TCA cycle metabolites were detected. However, these organic acids only accounted for 1.9-57.6% of the acids produced. Up to 80 mM fluoride hardly inhibited growth and did not inhibit acid production except for <i>C.</i> <i>glabrata</i>, despite the low 50% inhibitory fluoride concentration of 0.19-0.34 mM for enolase.</p><p><strong>Conclusion: </strong><i>Candida</i> species produced acids under aerobic and anaerobic conditions, indicating their significant cariogenicity. Their growth and acid production were highly fluoride-resistant, whereas their enolase was fluoride-sensitive, suggesting mechanisms for maintaining low intracellular fluoride. The mechanisms underlying the fluoride resistance remain underexplored. Approaches other than fluoride may be needed to control <i>Candida</i>-associated caries.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2473938"},"PeriodicalIF":3.7,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884091/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In vitro</i> modulation of proinflammatory and proteolytic activities of <i>Porphyromonas gingivalis</i> by selected lactobacilli.","authors":"Johana R Naja, Leyla Desparois, Elvira M Hebert, Maria Elena Fátima Nader, Lucila Saavedra, Carlos J Minahk, Vanessa P Houde","doi":"10.1080/20002297.2025.2469894","DOIUrl":"10.1080/20002297.2025.2469894","url":null,"abstract":"<p><strong>Objective: </strong>The aim of the present study was to characterize the antimicrobial and anti-inflammatory activities of postbiotics from lactic acid bacteria against <i>Porphyromonas gingivalis</i>.</p><p><strong>Material and methods: </strong>The anti-<i>P. gingivalis</i> activity of postbiotics from the CERELA culture collection was assessed by measuring changes in the expression of key host proteins by ELISA and qPCR, the proteolytic activity by a fluorescence and a spectrophotometric method and virulence factors from <i>P.</i> <i>gingivalis</i> by qPCR.</p><p><strong>Results: </strong>Even though <i>Lacticaseibacillus (L.) rhamnosus</i> CRL1522 and <i>Lactiplantibacillus (L.) plantarum</i> CRL1363 exhibit only a discrete antibacterial activity against <i>P. gingivalis</i>, the cell-free supernatants of these strains significantly reduced <i>P. gingivalis</i>-induced secretion of interleukins IL-6 and IL-8 by keratinocytes and TNF-α and IL-6 by U937 macrophage-like cells. More importantly, <i>P. gingivalis</i> arginine-gingipain (Rgp) protease activity was markedly reduced by both lactic acid bacteria (LAB) strains. This finding is particularly interesting because it means that both LAB might prevent the ulterior citrullination of peptides and the consequent generation of autoantibodies. The expression of <i>COX2</i> and <i>TLR2</i> was also significantly downregulated in macrophages.</p><p><strong>Conclusion: </strong>Postbiotics from <i>L. rhamnosus</i> CRL1522 and <i>L. plantarum</i> CRL1363 rise as suitable candidates for antagonizing the periodontopathogen <i>P. gingivalis</i>, since they were able to reduce the expression of proinflammatory cytokines and the protein degradation induced by this pathogen. We propose that postbiotics from these LAB could potentially halt the progression of periodontitis based on this <i>in vitro</i> study.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2469894"},"PeriodicalIF":3.7,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11864006/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}