Journal of Oral Microbiology最新文献

{"title":"Combined oral and peripheral blood detection of <i>Porphyromonas</i> gingivalis associates with coronary heart disease and gut microbiota alterations in a coronary angiography cohort.","authors":"Yingle Wu, Longlong Zheng, Chaoli Song, Ke Wang, Laijing Du, Yalei Chang, Yanhui Han, Bei Liu, Xiaopeng Qu","doi":"10.1080/20002297.2026.2712022","DOIUrl":"https://doi.org/10.1080/20002297.2026.2712022","url":null,"abstract":"<p><strong>Background: </strong>Periodontal pathogens are associated with cardiovascular disease, but clinical evidence linking Porphyromonas gingivalis (Pg) detection with coronary heart disease (CHD) and gut microbiota features remains limited. This study evaluated associations between oral and peripheral blood Pg detection status, CHD prevalence and exploratory gut microbiota characteristics in participants undergoing coronary angiography (CAG).</p><p><strong>Materials and methods: </strong>This analytical cross-sectional study included 165 CAG participants, including 124 patients with CAG-confirmed CHD and 41 non-CHD controls. Oral and peripheral blood Pg were detected by TaqMan quantitative polymerase chain reaction. Modified Poisson regression with robust standard errors was used to estimate adjusted prevalence ratios (PRs). Gut microbiota profiles were assessed by 16S rRNA gene sequencing.</p><p><strong>Results: </strong>Oral Pg positivity was more frequent in CHD participants than in non-CHD controls and remained associated with CHD in the primary adjusted model (PR = 1.237, 95% CI 1.044-1.465). The oral+/blood+ group showed the strongest association with CHD prevalence (PR = 1.362, 95% CI 1.099-1.688). Five FDR-significant ASVs were enriched in oral Pg-positive participants, whereas predicted pathways were not significant after FDR correction.</p><p><strong>Conclusions: </strong>Oral Pg positivity, especially combined oral and peripheral blood Pg nucleic acid positivity, was associated with CHD prevalence in this CAG-defined cohort.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2712022"},"PeriodicalIF":6.7,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13474525/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148764940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperuricemia is associated with periodontal ligature dysbiosis in a murine model of experimental periodontitis.","authors":"Zhicong Wu, Tiantian Xu, Li Zhao, Yinghong Zhou, Ting Yu","doi":"10.1080/20002297.2026.2716991","DOIUrl":"https://doi.org/10.1080/20002297.2026.2716991","url":null,"abstract":"<p><strong>Background: </strong>Hyperuricemia (Hu) is a systemic metabolic disorder implicated in inflammatory conditions and has been epidemiologically associated with an increased prevalence of periodontitis. However, whether Hu is associated with periodontal microbial dysbiosis in experimental periodontitis remains unclear.</p><p><strong>Objective: </strong>This exploratory pilot study aimed to investigate whether Hu is associated with periodontal dysbiosis in experimental periodontitis by characterizing the diversity, taxonomic composition, and predicted functional potential of ligature-associated microbiota using 16S rRNA sequencing.</p><p><strong>Methods: </strong>A mouse model combining potassium oxonate-induced Hu with ligature-induced periodontitis was established. Male C57BL/6 mice were allocated to normouricemia with periodontitis (NuP), hyperuricemia with periodontitis (HuP), and allopurinol-treated hyperuricemia with periodontitis (HuP+Allo) groups. Ligature-associated microbiota were analyzed by 16S rRNA gene sequencing. Correlations between microbial alterations and biochemical parameters were evaluated using redundancy analysis.</p><p><strong>Results: </strong>Hu significantly increased microbial diversity and evenness, as indicated by higher Shannon index (<i>P</i> = 0.007) and Shannoneven index (<i>P</i> = 0.002), but did not affect community richness. At the phylum level, Hu increased the <i>Firmicutes</i>/<i>Bacteroidota</i> ratio and <i>Proteobacteria</i> abundance while decreasing <i>Bacteroidota</i> and <i>Actinobacteriota</i>. At the genus level, Hu enriched potentially pathogenic taxa, including <i>Streptococcus</i> and <i>Escherichia-Shigella</i>. Allopurinol partially reversed these changes. Redundancy analysis revealed that microbial shifts were significantly correlated with serum uric acid (<i>R²</i>  = 0.711, <i>P</i> = 0.040) and creatinine (<i>R²</i>  = 0.695, <i>P</i> = 0.035). Functional prediction suggested that Hu was associated with higher predicted abundances of NOD-like receptor signalling and lower predicted abundance of IL-17 signalling pathways.</p><p><strong>Conclusion: </strong>These exploratory findings suggest that Hu may be associated with dysbiotic remodeling of the periodontal ligature microbiota in experimental periodontitis, linking systemic metabolic disturbance with local microbial imbalance and inflammatory pathway alteration. These alterations were partially reversed by allopurinol and correlated with serum uric acid and creatinine, providing mechanistic insight into how Hu may aggravate periodontal tissue destruction.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2716991"},"PeriodicalIF":6.7,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13479511/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148794523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Letter to the Editor on: 'Association between dental caries and self-reported COPD prevalence in U.S. adults aged ≥60 years: a cross-sectional analysis of NHANES 2015-2020'.","authors":"Qingqing Shan","doi":"10.1080/20002297.2026.2715818","DOIUrl":"https://doi.org/10.1080/20002297.2026.2715818","url":null,"abstract":"","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2715818"},"PeriodicalIF":6.7,"publicationDate":"2026-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13470903/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148764251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral microbiome diversity and mortality in metabolic syndrome: a cohort study.","authors":"Lingfei Meng, Xiaohao Zhang, Xiaohua Zhuang, Wenpeng Cui","doi":"10.1080/20002297.2026.2712017","DOIUrl":"10.1080/20002297.2026.2712017","url":null,"abstract":"<p><strong>Background: </strong>Metabolic syndrome (MetS) is associated with a higher risk of mortality. Oral microbiome diversity is related to health outcomes, but its role in forecasting MetS prognosis and the relevant pathogenic mechanisms is still largely unexplored.</p><p><strong>Methods: </strong>Data from 1,769 MetS patients were extracted from the National Health and Nutrition Examination Survey (NHANES) 2009-2012 cycle. To evaluate alpha diversity, the Shannon index, Faith's phylogenetic diversity (PD), observed operational taxonomic units (OTUs), and the Inverse Simpson index were determined. ALDEx2 was employed for differential abundance analysis in the high-diversity subgroup. Co-occurrence network analysis was subsequently conducted, followed by partitioning around medoids (PAM) clustering for community typing. Prognostic associations were validated through Cox regression.</p><p><strong>Results: </strong>Both the Shannon index (hazard ratio [HR] = 0.78, 95% confidence interval [CI]: 0.63-0.96) and the Inverse Simpson index (HR = 0.84, 95% CI: 0.74-0.96) were inversely associated with mortality. In the high-diversity subgroup, differential abundance analysis did not identify any OTU whose relative abundance differed significantly by survival status. According to network analysis, the deceased group exhibited a higher density of positive co-occurrences (283 vs. 224 edges) alongside a notable expansion of negative interactions (71 vs. 15 edges), which was a hallmark of declining community stability. Two clusters were identified through community typing, yet this classification was not significantly related to survival outcomes (CType_2 vs. CType_1: HR = 1.05, 95% CI: 0.73-1.49).</p><p><strong>Conclusions: </strong>Oral microbiome diversity exhibits a negative association with mortality among MetS patients. However, in the high-diversity subgroup, ecological network disruption, rather than alpha diversity or differential taxonomic richness, differentiates the deceased patients from the alive counterparts. This suggests that community structure is a promising risk marker with a higher sensitivity than diversity alone.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2712017"},"PeriodicalIF":6.7,"publicationDate":"2026-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13449441/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148697675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral microbiota dysbiosis related to the cortical thinning and cognitive impairment in cerebral small vessel disease.","authors":"Yuerong Lizhu, Yiyi Chen, Xinze Zhang, Yuqi Luo, Zhizheng Zhuo, Jinhui Wang, Yunyun Duan, Li Chai, Jun Qiu, Ziyi Gao, Tingting Wang, Hongyi Yan, Xiaoyun Liang, Yilong Wang, Yingying Su, Ling Guan, Yaou Liu","doi":"10.1080/20002297.2026.2705667","DOIUrl":"10.1080/20002297.2026.2705667","url":null,"abstract":"<p><strong>Background: </strong>Prior studies have linked the microbiota to brain diseases, whereas the longitudinal effects of the oral microbiota on cortical thinning and cognitive impairments in cerebral small vessel disease (CSVD) remain unexplored.</p><p><strong>Methods: </strong>We recruited 120 CSVD patients and 40 healthy controls (HCs). The subgingival plaque microbiota was sequenced by a metagenomic approach. Cortical thickness was assessed using GM-centile, an age- and sex-normalized MRI metric. Differential microbial taxa and KEGG orthologs (KOs) between groups were identified using MaAsLin2. Associations between key differential taxa with CSVD-specific cortical thinning were examined using the Spearman test, and those with MoCA score and plasma inflammatory markers (CRP and lymphocyte counts) were examined by linear regression models. Mediation models evaluated the indirect role of cortical thinning in the relationship between microbial abundance and cognitive function. Generalized estimation equations validated the longitudinal effects of the microbiota on cortical thinning progression.</p><p><strong>Result: </strong>We identified distinct oral microbiota dysbiosis in CSVD, including depletion of <i>g_Selenomonas</i> and <i>g_Leptotrichia</i> and enrichment of <i>g_Treponema</i>. The abundance of these microbes was correlated with longitudinal cortical thinning in the frontal gyrus, insular lobes, and inferotemporal gyrus. Enrichment analysis revealed that CSVD-enriched KOs were linked to the upregulation of LPS-mediated pro-inflammatory pathways, while those depleted were associated with the reduced biosynthesis of neuroprotective short-chain fatty acids (SCFAs). <i>g_Leptotrichia</i> abundance showed negatively correlation with CRP (<i>p</i> = 0.045). Mediation analyses indicated that the association between <i>g_Leptotrichia</i> depletion and baseline cognitive impairment was mediated by bilateral insular cortical thinning (both <i>p</i> < 0.05). Additionally, the association between <i>g_Leptotrichia</i> depletion and one-year cognitive decline was mediated by superior frontal cortical thinning (<i>p</i> = 0.033).</p><p><strong>Conclusions: </strong>Oral microbiota dysbiosis in CSVD patients reflects a pro-inflammatory state, characterized by enhanced LPS synthesis and reduced SCFAs production. This dysbiosis is associated with CSVD-specific cortical thinning in regions vulnerable to neuroinflammation, which in turn mediates cognitive impairment.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2705667"},"PeriodicalIF":6.7,"publicationDate":"2026-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13446054/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148685023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Filtration-enriched metabolites and their association with salivary microbiota: a combined two-cohort analysis.","authors":"Yifan Zhang, Qinglong Wang, Yingying Zhai, Qi Wang, Zhenzhen Li, Yudong Liu, Shanshan Liu","doi":"10.1080/20002297.2026.2711156","DOIUrl":"10.1080/20002297.2026.2711156","url":null,"abstract":"<p><strong>Objective: </strong>We aimed to identify significantly reduced metabolites (SRMs) in saliva by filtration and to analyze their associations with oral microorganisms.</p><p><strong>Methods: </strong>A total of 423 volunteers were assigned into two cohorts. Paired saliva samples were collected from cohort 1 (<i>n</i> = 60) and underwent metabolomics analysis before and after filtration. SRMs were identified based on the following thresholds: variable importance in projection ≥1, false discovery rate <0.05, and fold change ≥10. The pre-filtration samples from Cohort 1 were subjected to microbiome analysis. Saliva samples collected from cohort 2 (<i>n</i> = 334) underwent metabolomic and microbiome analyses, but were not filtered.</p><p><strong>Results: </strong>Principal coordinates analysis revealed a clear separation between pre- and post-filtration samples. The filtered saliva samples had approximately 5.8% fewer detectable metabolites. Notably, over half of the SRMs had an unknown origin, indicating significant knowledge gaps in oral metabolites. <i>Prevotella melaninogenica</i> and <i>Veillonella parvula</i> were core species associated with the SRMs (hypoxanthine and phosphatidylcholine), with purine metabolism identified as enriched pathway for <i>Prevotella melaninogenica</i>.</p><p><strong>Conclusions: </strong>Filtration can reshape saliva metabolite profiles. This study combined key metabolite filtration with integrated multi-omics and functional analyses, providing new insights into saliva metabolism and microbe-metabolite interactions.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2711156"},"PeriodicalIF":6.7,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13439783/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148678973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Culture-based analysis of oral dysbiosis in post-oncological patients rehabilitated with obturator prostheses.","authors":"Adam Bęben, Katarzyna Juszczuk, Ewa Kwapisz, Oliwia Chojnowska, Iwona Ordyniec-Kwaśnica, Katarzyna Garbacz","doi":"10.1080/20002297.2026.2698177","DOIUrl":"10.1080/20002297.2026.2698177","url":null,"abstract":"<p><strong>Background: </strong>Post-oncological maxillary defects are commonly rehabilitated with obturator prostheses. This study compared the oral microbiota of post-oncological patients using obturators with that of control subjects wearing conventional removable dentures, focusing on clinically relevant, cultivable microorganisms.</p><p><strong>Methods: </strong>Post-oncological obturator users and denture-wearing controls were examined. Swabs from the oral mucosa, prosthesis surfaces, and resection sites were analyzed using culture-based microbiological methods, including species identification and antimicrobial susceptibility testing. Microbial, demographic, and clinical data were statistically analyzed.</p><p><strong>Results: </strong>Obturator users harbored a more pathogenic and antimicrobial-resistant oral microbiota than controls, with increased colonization by opportunistic pathogens such as <i>Staphylococcus aureus</i> (including MRSA), Gram-negative rods (<i>Klebsiella pneumoniae</i>), and yeast-like fungi. <i>S. aureus</i> remained significantly more prevalent in obturator users regardless of radiotherapy status. <i>Candida</i> spp. were prevalent in both groups, with <i>Candida albicans</i> predominating and <i>Candida tropicalis</i> more frequent among obturator users.</p><p><strong>Conclusions: </strong>Culture-based analysis demonstrated increased colonization with antimicrobial-resistant opportunistic pathogens in post-oncological patients using obturator prostheses, highlighting the need for intensified hygiene and preventive care.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2698177"},"PeriodicalIF":6.7,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13446048/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148684864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PBM's effects on plaque microbiome for periodontal-orthodontic patients during retention.","authors":"Guanning Zhang, Yufeng Zhang, Shi Huang, Colman McGrath, Yanqi Yang, Zhiyi Shan","doi":"10.1080/20002297.2026.2693412","DOIUrl":"10.1080/20002297.2026.2693412","url":null,"abstract":"<p><strong>Objectives: </strong>This pilot study aimed to determine whether adjunctive photobiomodulation (PBM) alters dental plaque microbiota and clinical parameters in periodontitis patients during orthodontic retention.</p><p><strong>Methods: </strong>Six stage IV, grade C periodontitis patients entering orthodontic retention were enrolled in this split-mouth study. One arch side received monthly PBM therapy (GaAlAs diode laser) for six months; the contralateral side received sham irradiation. Periodontal examinations were performed. Supragingival and subgingival plaque samples were evaluated at appliance removal (T_i), 6 months (T_ii) and 12 months (T_iii) using 16S rRNA sequencing.</p><p><strong>Results: </strong>No significant differences were observed in periodontal clinical parameters between PBM and placebo sites at any time point (<i>P</i> > 0.05). Microbiome analysis showed similar alpha and beta diversity in supragingival and subgingival microbiota between the groups (<i>P</i> > 0.05). Exploratory genus-level differences were limited, with <i>Treponema</i> enriched in placebo supragingival plaque and <i>Aggregatibacter</i> in placebo subgingival plaque at T_ii, while <i>Catonella</i> differed in time-pooled supragingival comparisons. No headline differential genera were detected at T_i and T_iii.</p><p><strong>Conclusion: </strong>Adjunctive PBM did not significantly alter overall plaque microbial community structure or clinical periodontal parameters. However, a few periodontitis-associated genera showed nominal, exploratory differences. These hypothesis-generating signals warrant confirmation in adequately powered trials.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2693412"},"PeriodicalIF":6.7,"publicationDate":"2026-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13431019/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148669548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From biological association to service integration: a public administration perspective on oral microbiota and chronic kidney disease.","authors":"Yuyun Eka Kartika Sari, Deby Febriyan Eprilianto, Ahmad Yusroni, Muhammad Khairul Anwar","doi":"10.1080/20002297.2026.2704279","DOIUrl":"10.1080/20002297.2026.2704279","url":null,"abstract":"","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2704279"},"PeriodicalIF":6.7,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13425535/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148653985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salivary mycobial profiles of oral cancer patients - a cohort study.","authors":"Anna I Mäkinen, Derek W K van der Plas, Mark J Buijs, Antti A Mäkitie, Jukka H Meurman, Egija Zaura, Bernd W Brandt","doi":"10.1080/20002297.2026.2696598","DOIUrl":"10.1080/20002297.2026.2696598","url":null,"abstract":"<p><strong>Background: </strong>Several studies have investigated the possible links between oral yeast infections and oral cancer. However, many of these studies concentrate on single species or genera and the overall oral mycobial profiles of oral cancer patients are not well known.</p><p><strong>Objective: </strong>We examined the salivary mycobial profiles of oral squamous cell carcinoma (OSCC) patients before and after cancer treatment and compared them with those of non-cancer controls. Additionally, we tested the recently introduced concept of two salivary mycotypes-one driven by the genus <i>Candida</i> and one by the genus <i>Malassezia</i>-in the context of OSCC.</p><p><strong>Design: </strong>Paraffin-stimulated saliva from OSCC patients before cancer treatment (<i>n </i>= 75), after a mean follow-up of 4 years (<i>n </i>= 27) and from non-cancer controls (<i>n </i>= 81) was analysed using ITS2 amplicon sequencing.</p><p><strong>Results: </strong>The OSCC patients had lower alpha-diversity (<i>p</i> = 0.001, Shannon diversity index) and higher relative abundance of OTU1 <i>Candida albicans</i> compared with controls. No significant difference in beta-diversity or in differential abundance of OTUs was found between the paired pre-treatment OSCC and follow-up samples or the follow-up samples and controls. Baseline OSCC patients had higher prevalence of the <i>Candida-</i>mycotype compared with controls (<i>p</i> = 0.019). The prevalence of the <i>Malassezia</i>-mycotype increased from baseline OSCC to follow-up.</p><p><strong>Conclusions: </strong>Our results suggest that OSCC patients have a <i>Candida</i>-dominated salivary mycobiota that differs significantly from that of non-cancer controls and shifts towards an increased prevalence of <i>Malazzesia</i> after cancer is treated.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2696598"},"PeriodicalIF":6.7,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13425524/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148653963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}