Journal of Oral Microbiology最新文献_第6页

Manipulating the diseased oral microbiome: the power of probiotics and prebiotics. 操纵患病的口腔微生物群：益生菌和益生元的力量。

IF 4.5 2区医学

Journal of Oral Microbiology Pub Date : 2024-01-31 eCollection Date: 2024-01-01 DOI: 10.1080/20002297.2024.2307416

X Yu, D A Devine, J J Vernon

{"title":"Manipulating the diseased oral microbiome: the power of probiotics and prebiotics.","authors":"X Yu, D A Devine, J J Vernon","doi":"10.1080/20002297.2024.2307416","DOIUrl":"https://doi.org/10.1080/20002297.2024.2307416","url":null,"abstract":"Dental caries and periodontal disease are amongst the most prevalent global disorders. Their aetiology is rooted in microbial activity within the oral cavity, through the generation of detrimental metabolites and the instigation of potentially adverse host immune responses. Due to the increasing threat of antimicrobial resistance, alternative approaches to readdress the balance are necessary. Advances in sequencing technologies have established relationships between disease and oral dysbiosis, and commercial enterprises seek to identify probiotic and prebiotic formulations to tackle preventable oral disorders through colonisation with, or promotion of, beneficial microbes. It is the metabolic characteristics and immunomodulatory capabilities of resident species which underlie health status. Research emphasis on the metabolic environment of the oral cavity has elucidated relationships between commensal and pathogenic organisms, for example, the sequential metabolism of fermentable carbohydrates deemed central to acid production in cariogenicity. Therefore, a focus on the preservation of an ecological homeostasis in the oral environment may be the most appropriate approach to health conservation. In this review we discuss an ecological approach to the maintenance of a healthy oral environment and debate the potential use of probiotic and prebiotic supplementation, specifically targeted at sustaining oral niches to preserve the delicately balanced microbiome.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"16 1","pages":"2307416"},"PeriodicalIF":4.5,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10833113/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139672029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial effect of probiotic bacteriocins on Streptococcus mutans biofilm in a dynamic oral flow chamber model - an in vitro study. 益生菌细菌素在动态口腔流室模型中对变异链球菌生物膜的抗菌作用--一项体外研究。

IF 3.7 2区医学

Journal of Oral Microbiology Pub Date : 2024-01-28 eCollection Date: 2024-01-01 DOI: 10.1080/20002297.2024.2304971

Elisabeth Reichardt, Viktoriya Shyp, Lea Alig, Carlalberta Verna, Eva M Kulik, Michael M Bornstein

{"title":"Antimicrobial effect of probiotic bacteriocins on Streptococcus mutans biofilm in a dynamic oral flow chamber model - an in vitro study.","authors":"Elisabeth Reichardt, Viktoriya Shyp, Lea Alig, Carlalberta Verna, Eva M Kulik, Michael M Bornstein","doi":"10.1080/20002297.2024.2304971","DOIUrl":"10.1080/20002297.2024.2304971","url":null,"abstract":"Aim: To determine the antimicrobial activity of the bacteriocin-producing probiotic strains Streptococcus salivarius K12 and Streptococcus salivarius M18 alone or in combination against caries-associated Streptococcus mutans.Methods: Antimicrobial activity of S. salivarius K12 and/or S. salivarius M18 against S. mutans ATCC 25175 growth and biofilm formation on hydroxyapatite (HA) discs was determined in a flow chamber model by recording the colony forming units (CFU/ml) after 48 h of co-cultivation. The biofilm was analyzed by scanning electron microscopy (SEM) and by confocal laser scanning microscopy (CLSM). Additionally, the simultaneous antagonism assay was used to assess the inhibitory effect of S. salivarius K12 and/or S. salivarius M18 against S. mutans ATCC 25175 and 21 clinical isolates of S. mutans.Results: Co-cultivation of S. mutans and S. salivarius K12 and/or S. salivarius M18 led to the inhibition of S. mutans viability, thereby, preventing its biofilm formation on HA discs. Furthermore, S. salivarius K12 and S. salivarius M18 exhibited antimicrobial activity against most clinical isolates of S. mutans.Conclusion: The in vitro flow chamber system used in this study allows the simulation of time-dependent administration of S. salivarius probiotic strains, either alone or in combination, to investigate the prevention of S. mutans biofilm formation in a standardized model.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"16 1","pages":"2304971"},"PeriodicalIF":3.7,"publicationDate":"2024-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10823884/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139577341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effective disinfecting of negative pressure pipelines of DCUs reduces the risk of cross infection in dental care. 对 DCU 负压管道进行有效消毒可降低牙科护理中交叉感染的风险。

IF 4.5 2区医学

Journal of Oral Microbiology Pub Date : 2024-01-07 eCollection Date: 2024-01-01 DOI: 10.1080/20002297.2023.2299538

Xuerong Lv, Ke Yan, Xiang Han, Feiyang Wang, Yuzhuo Ma, Shougang Zhang, Xiaoqian Wang

{"title":"Effective disinfecting of negative pressure pipelines of DCUs reduces the risk of cross infection in dental care.","authors":"Xuerong Lv, Ke Yan, Xiang Han, Feiyang Wang, Yuzhuo Ma, Shougang Zhang, Xiaoqian Wang","doi":"10.1080/20002297.2023.2299538","DOIUrl":"https://doi.org/10.1080/20002297.2023.2299538","url":null,"abstract":"Objectives: Microbial contamination of various accessory parts of the dental chair units (DCUs) is an essential source of cross infection, while the accessories of the crucial suction function are usually overlooked. In this study, we aim to find an effective disinfectant and a cost-effective method to remove bacterioplankton and bacterial biofilm deposited in the negative pressure suction pipelines to control cross infection during dental treatment.Methods: Double-chain quaternary ammonium salt disinfectant (Orotol Plus®), 3% hydrogen peroxide solution plus multi-enzyme cleaning agent and chlorine disinfectant are used to clean and disinfect the negative pressure pipelines of DCUs. Microbiological examinations, air condition detection, corrosion tests and gene sequencing are performed.Results: Little bacteria grow in the pipelines disinfected with double-chain quaternary ammonium salt disinfectants, destruction of biofilms in these pipelines appears, and multi-resistant bacteria cannot be detected. Minimal damage to metal sheets and fittings is caused by double-chain quaternary ammonium salt disinfectants.Conclusion: Double-chain quaternary ammonium salt disinfectant has excellent bactericidal ability and anti-biofilm effect, and it is less corrosive to the fittings of the pipelines. Thus, the double-chain quaternary ammonium salt disinfectant is a potential novel disinfectant for negative pressure suction pipelines of DCUs to control cross infection during dental treatment.Clinical significance: It is essential to add all these data to our dental practice to control cross infection with a broader landscape.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"16 1","pages":"2299538"},"PeriodicalIF":4.5,"publicationDate":"2024-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10773641/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139403242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

N-acyl homoserine lactones lactonase est816 suppresses biofilm formation and periodontitis in rats mediated by Aggregatibacter actinomycetemcomitans. N-acyl homoserine lactones lactonase est816 可抑制放线杆菌介导的大鼠生物膜形成和牙周炎。

IF 4.5 2区医学

Journal of Oral Microbiology Pub Date : 2024-01-07 eCollection Date: 2024-01-01 DOI: 10.1080/20002297.2023.2301200

Zelda Ziyi Zhao, Junmin Wang, Xinpai Liu, Zezhi Wang, Xianyu Zheng, Wuli Li, Tianfan Cheng, Jing Zhang

{"title":"N-acyl homoserine lactones lactonase est816 suppresses biofilm formation and periodontitis in rats mediated by Aggregatibacter actinomycetemcomitans.","authors":"Zelda Ziyi Zhao, Junmin Wang, Xinpai Liu, Zezhi Wang, Xianyu Zheng, Wuli Li, Tianfan Cheng, Jing Zhang","doi":"10.1080/20002297.2023.2301200","DOIUrl":"https://doi.org/10.1080/20002297.2023.2301200","url":null,"abstract":"Aims: The current study aimed to explore the adjuvant therapeutic effect of N-acyl homoserine lactones (AHLs)-lactonase est816 on Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) biological behaviors and periodontitis progression.Methods: The inhibitory properties of est816 were detected by live/dead bacterial staining, scanning electron microscope (SEM), crystal-violet staining and reverse-transcription quantitative PCR (RT-qPCR). Biocompatibility of est816 on human gingival fibroblasts (HGFs) and human gingival epithelial cells (HGEs) was evaluated by CCK8 and ELISA. The ligature-induced periodontitis model was established in rats. Micro computed tomography and immunohistochemical and histological staining served to evaluate the effect of est816 on the prevention of periodontitis in vivo.Results: est816 significantly attenuated biofilm formation, reduced the mRNA expression of cytolethal distending toxin, leukotoxin and poly-N-acetyl glucosamine (PNAG) and downregulated expressions of interleukin-6 and tumor necrosis factor-α with low cell toxicity. In vivo investigations revealed est816 decreased alveolar bone resorption, suppressed matrix metalloproteinase-9 expression and increased osteoprotegerin expression.Conclusion: est816 inhibited A. actinomycetemcomitans biofilm formation and virulence release, resulting in anti-inflammation and soothing of periodontitis in rats, indicating that est816 could be investigated in further research on periodontal diseases.","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"16 1","pages":"2301200"},"PeriodicalIF":4.5,"publicationDate":"2024-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10773656/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139403241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Epstein-Barr virus infection in primary junctional epithelial cell cultures 在原代交界上皮细胞培养物中检测 Epstein-Barr 病毒感染

IF 4.5 2区医学

Journal of Oral Microbiology Pub Date : 2024-01-03 DOI: 10.1080/20002297.2023.2301199

Lilit Tonoyan, Charles V Olivieri, Marlène Chevalier, Robert Marsault, Alain Doglio

引用次数: 0

The importance of biofilm contamination control for dental unit waterlines: a multicenter assessment of the microbiota diversity of biofilm in dental unit waterlines 牙科单位水管生物膜污染控制的重要性：牙科单位水管生物膜微生物群多样性的多中心评估

IF 4.5 2区医学

Journal of Oral Microbiology Pub Date : 2023-12-30 DOI: 10.1080/20002297.2023.2299496

Piaopiao Chen, Jiang Zeng, Feiruo Hong, Cong Li, Huimin Wang, Xuefen Yu

引用次数: 0

MAT2A inhibition suppresses inflammation in Porphyromonas gingivalis-infected human gingival fibroblasts 抑制 MAT2A 可抑制受牙龈卟啉单胞菌感染的人牙龈成纤维细胞的炎症反应

IF 4.5 2区医学

Journal of Oral Microbiology Pub Date : 2023-12-15 DOI: 10.1080/20002297.2023.2292375

Lishan Jiang, Jingwen Li, Kun Ji, Lang Lei, Houxuan Li

引用次数: 0

Organotypic model of the gingiva for studying bacterial and viral pathogens implicated in periodontitis 研究牙周炎细菌和病毒病原体的牙龈组织模型

IF 4.5 2区医学

Journal of Oral Microbiology Pub Date : 2023-12-12 DOI: 10.1080/20002297.2023.2292382

Anna Golda, Anna Gasiorek, Ewelina Dobosz, Zuzanna Oruba, Richard J. Lamont, Jan Potempa, Joanna Koziel

引用次数: 0

Oxidative stress response: a critical factor affecting the ecological competitiveness of Streptococcus mutans 氧化应激反应：影响变异链球菌生态竞争力的关键因素

IF 4.5 2区医学

Journal of Oral Microbiology Pub Date : 2023-12-12 DOI: 10.1080/20002297.2023.2292539

Xingqun Cheng, Xin Xu, Xuedong Zhou, Jia Ning

引用次数: 0

Deficiency of dltD contributes to increased autolysis in Streptococcus mutans 缺乏 dltD 会导致变异链球菌自溶增加

IF 4.5 2区医学

Journal of Oral Microbiology Pub Date : 2023-12-10 DOI: 10.1080/20002297.2023.2290221

Jingyun Du, Shan Huang, Yijun Li, Minjing Wu, Shuai Chen, Ling Zhan, Xiaojing Huang

引用次数: 0