Platelet-rich fibrin obtained from different protocols affects the formation of the in vitro multispecies subgingival biofilm associated with periodontitis.
Fabio Hideaki Uyeda, Gustavo Quilles Vargas, Larissa Matias Malavazi, Tatiane Tiemi Macedo, Aline Paim de Abreu Paulo Gomes, Manuela Rocha Bueno, Pedro Henrique Moreira Paulo Tolentino, Lucas Daylor Aguiar da Silva, Luciene Cristina Figueiredo, Jamil Awad Shibli, Bruno Bueno-Silva
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Abstract
Background: The aim of this article is to evaluate the effect of different portions of Platelet Rich Fibrin (PRF) membranes and liquid-PRF, prepared by two distinct protocols/centrifuges each, on the multispecies subgingival biofilm.
Materials and methods: PRF membranes and liquid-PRF were prepared using two protocols: centrifuge 1 uses fixed acceleration while centrifuge 2, progressive acceleration. PRF samples were introduced into device concurrently with 33-species bacterial inoculum. After seven days, biofilm metabolic activity (MA) and microbial profile were evaluated through colorimetric reaction and DNA-DNA hybridization, respectively.
Results: Among PRF membranes, the ones from centrifuge 1 led to better reduction in MA, total biofilm, and F. periodonticum, P. gingivalis and T. forsythia counts when compared to untreated/centrifuge 2 treated biofilms. However, centrifuge 2 liquid-PRF reduced MA, total biofilm and F. periodonticum counts when compared to untreated/centrifuge 1 treated-biofilms.
Conclusion: PRF membrane and exhibited comparable antibiofilm activity. However, PRF distinct forms, obtained by same centrifugation protocol, may present different antimicrobial properties.
期刊介绍：
As the first Open Access journal in its field, the Journal of Oral Microbiology aims to be an influential source of knowledge on the aetiological agents behind oral infectious diseases. The journal is an international forum for original research on all aspects of ''oral health''. Articles which seek to understand ''oral health'' through exploration of the pathogenesis, virulence, host-parasite interactions, and immunology of oral infections are of particular interest. However, the journal also welcomes work that addresses the global agenda of oral infectious diseases and articles that present new strategies for treatment and prevention or improvements to existing strategies.
Topics: ''oral health'', microbiome, genomics, host-pathogen interactions, oral infections, aetiologic agents, pathogenesis, molecular microbiology systemic diseases, ecology/environmental microbiology, treatment, diagnostics, epidemiology, basic oral microbiology, and taxonomy/systematics.
Article types: original articles, notes, review articles, mini-reviews and commentaries
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